AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene047642

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047642.t1 MTR_5g017500 85.830 247 17 2 1 247 1 229 5.61e-148 412
MS.gene047642.t1 MTR_4g117990 45.872 109 57 1 14 122 36 142 3.94e-27 107
MS.gene047642.t1 MTR_7g081190 49.020 102 49 2 19 118 43 143 9.70e-22 93.2
MS.gene047642.t1 MTR_1g061600 31.111 135 74 4 22 143 133 261 1.43e-15 75.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene047642.t1 AT3G24860 34.572 269 130 7 11 246 54 309 7.31e-44 150
MS.gene047642.t1 AT2G44730 44.348 115 54 2 9 119 51 159 1.25e-22 95.5
MS.gene047642.t1 AT3G10030 35.644 101 59 2 22 122 161 255 9.68e-15 73.6
MS.gene047642.t1 AT3G10030 35.644 101 59 2 22 122 161 255 1.04e-14 73.6
MS.gene047642.t1 AT3G54390 26.522 230 139 4 22 230 38 258 1.10e-14 72.4
MS.gene047642.t1 AT3G54390 26.522 230 139 4 22 230 54 274 1.39e-14 72.4
MS.gene047642.t1 AT3G58630 30.769 117 68 2 22 128 26 139 1.18e-13 69.7
MS.gene047642.t1 AT3G58630 30.769 117 68 2 22 128 26 139 1.19e-13 69.7
MS.gene047642.t1 AT3G14180 34.043 94 57 3 22 115 84 172 5.97e-11 62.4

Find 80 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTGTTACTGTTGGTTTC+TGG 0.153076 5.3:-9792802 None:intergenic
GTAAGCTCGAATGAGGTTAA+TGG 0.275887 5.3:-9792399 None:intergenic
GATTCCATTGGAAAGGCATT+TGG 0.308166 5.3:+9793031 MS.gene047642:CDS
ACGACATTGAAGAGCGGTTT+TGG 0.311108 5.3:-9792528 None:intergenic
GAATGAGGTTAATGGTTTCT+TGG 0.338119 5.3:-9792391 None:intergenic
TTCTTGGTGGGTCCATGGGA+TGG 0.355899 5.3:-9792375 None:intergenic
CCGTCGCTATCGTCGGAGAT+AGG 0.363885 5.3:-9792713 None:intergenic
TCTTGGTGGGTCCATGGGAT+GGG 0.368132 5.3:-9792374 None:intergenic
GATTAGAGATGGAGAATAAG+AGG 0.371813 5.3:+9792972 MS.gene047642:CDS
ATTCCATTGGAAAGGCATTT+GGG 0.374598 5.3:+9793032 MS.gene047642:CDS
ATGGTTTCTTGGTGGGTCCA+TGG 0.378553 5.3:-9792380 None:intergenic
TTTCAGATCGGTGGCGTTGA+CGG 0.386482 5.3:-9792568 None:intergenic
GCCCACTCCGCGGCAGTCAA+TGG 0.394607 5.3:+9792450 MS.gene047642:CDS
ATGGGTTGAGGTTTCTTTGT+GGG 0.395257 5.3:-9792356 None:intergenic
GAAAGAGGTCGGACGGAGAT+AGG 0.403361 5.3:-9792680 None:intergenic
CCGCCGGTTCTTCTCGATCC+TGG 0.419395 5.3:+9792615 MS.gene047642:CDS
TGGAGGATTGTTGATTCCAT+TGG 0.426166 5.3:+9793019 MS.gene047642:CDS
TGGGTCCATGGGATGGGTTG+AGG 0.432168 5.3:-9792368 None:intergenic
AGCTTTGCAGAGAGGATTGT+AGG 0.434747 5.3:+9792902 MS.gene047642:CDS
TGGTTTCTTGGTGGGTCCAT+GGG 0.436356 5.3:-9792379 None:intergenic
ATAGTGCTGCTAGGTCTGTT+AGG 0.441997 5.3:+9792753 MS.gene047642:CDS
TAAGAGAATACCATTTCTCT+TGG 0.442052 5.3:-9792421 None:intergenic
CCCATTGACTGCCGCGGAGT+GGG 0.449342 5.3:-9792451 None:intergenic
TTCGAGCTTACCAAGAGAAA+TGG 0.455451 5.3:+9792411 MS.gene047642:CDS
TGCAGAGAGGATTGTAGGGT+TGG 0.456384 5.3:+9792907 MS.gene047642:CDS
GAGGTTAATGGTTTCTTGGT+GGG 0.461484 5.3:-9792387 None:intergenic
TGTTGTTGTCTTGTTACTGT+TGG 0.461863 5.3:-9792809 None:intergenic
CCCACTCCGCGGCAGTCAAT+GGG 0.462147 5.3:+9792451 MS.gene047642:CDS
AGAGAGGTTTAGATTAGAGA+TGG 0.466781 5.3:+9792961 MS.gene047642:CDS
GATGGGTTGAGGTTTCTTTG+TGG 0.466878 5.3:-9792357 None:intergenic
TTGAGGTTTCTTTGTGGGAA+GGG 0.468718 5.3:-9792351 None:intergenic
CTGCCAGGATCGAGAAGAAC+CGG 0.473658 5.3:-9792618 None:intergenic
TCCCATTGACTGCCGCGGAG+TGG 0.479922 5.3:-9792452 None:intergenic
GTATGATACTTGAATCTCAA+TGG 0.493726 5.3:+9792999 MS.gene047642:CDS
TCTCTTGGTAAGCTCGAATG+AGG 0.503486 5.3:-9792406 None:intergenic
TGAGGTTAATGGTTTCTTGG+TGG 0.505014 5.3:-9792388 None:intergenic
CGTCGCTATCGTCGGAGATA+GGG 0.505081 5.3:-9792712 None:intergenic
GCTTTGCAGAGAGGATTGTA+GGG 0.507408 5.3:+9792903 MS.gene047642:CDS
GGAAGGGAAAGAGTGAGTTC+TGG 0.514086 5.3:-9792335 None:intergenic
TCTTCAATGTCGTCATAAGA+TGG 0.539576 5.3:+9792538 MS.gene047642:CDS
GATTGTTGATTCCATTGGAA+AGG 0.539782 5.3:+9793024 MS.gene047642:CDS
TAAGAAGATGGAGATGATGA+AGG 0.556347 5.3:+9792934 MS.gene047642:CDS
CGGCAGTCAATGGGAAGAAG+TGG 0.558439 5.3:+9792460 MS.gene047642:CDS
ATAGGGTGCTTCTGAGAAAG+AGG 0.564002 5.3:-9792695 None:intergenic
GTTGAGGTTTCTTTGTGGGA+AGG 0.565690 5.3:-9792352 None:intergenic
GGATCGAGAAGAACCGGCGG+AGG 0.569200 5.3:-9792612 None:intergenic
GGTCGGACGGAGATAGGTAA+AGG 0.569635 5.3:-9792674 None:intergenic
AGGGTTGGAAAATAAGAAGA+TGG 0.571203 5.3:+9792922 MS.gene047642:CDS
AGAAACCTCAACCCATCCCA+TGG 0.573775 5.3:+9792363 MS.gene047642:CDS
CTTCTTCCCATTGACTGCCG+CGG 0.577676 5.3:-9792457 None:intergenic
CTGCTAGGTCTGTTAGGTCA+AGG 0.578720 5.3:+9792759 MS.gene047642:CDS
CCGACGATAGCGACGGTGAG+AGG 0.582725 5.3:+9792720 MS.gene047642:CDS
CTCACCGCTACTTCCTCCGC+CGG 0.582798 5.3:+9792599 MS.gene047642:CDS
CCTCTCACCGTCGCTATCGT+CGG 0.588877 5.3:-9792720 None:intergenic
AGACCCAAATGCCTTTCCAA+TGG 0.589025 5.3:-9793035 None:intergenic
CGGAGGAAGTAGCGGTGAGG+CGG 0.599547 5.3:-9792595 None:intergenic
CGACGATAGCGACGGTGAGA+GGG 0.604292 5.3:+9792721 MS.gene047642:CDS
AGTGAGTTCTGGTGAAGCGG+TGG 0.606604 5.3:-9792324 None:intergenic
CATGAGACGAAAATACTGCC+AGG 0.607104 5.3:-9792633 None:intergenic
AAGAGTGAGTTCTGGTGAAG+CGG 0.610541 5.3:-9792327 None:intergenic
CTTCTGAGAAAGAGGTCGGA+CGG 0.613702 5.3:-9792687 None:intergenic
AGTTGAGAAGCTTTGCAGAG+AGG 0.619704 5.3:+9792894 MS.gene047642:CDS
TGATGAAGAGGAGGGAGTGA+TGG 0.625795 5.3:+9792853 MS.gene047642:CDS
GGTGCTTCTGAGAAAGAGGT+CGG 0.636676 5.3:-9792691 None:intergenic
AGAACCGGCGGAGGAAGTAG+CGG 0.637741 5.3:-9792603 None:intergenic
AGATGATGAAGGAAACAGAG+AGG 0.638850 5.3:+9792945 MS.gene047642:CDS
TGAAACTGATGATGAAGAGG+AGG 0.646384 5.3:+9792844 MS.gene047642:CDS
GAAACTGATGATGAAGAGGA+GGG 0.650226 5.3:+9792845 MS.gene047642:CDS
TGATGAAACTGATGATGAAG+AGG 0.652723 5.3:+9792841 MS.gene047642:CDS
TGATACTTGAATCTCAATGG+AGG 0.662965 5.3:+9793002 MS.gene047642:CDS
CTCATGGATGATCTTGAACG+CGG 0.669974 5.3:+9792650 MS.gene047642:CDS
CTTATGACGACATTGAAGAG+CGG 0.678566 5.3:-9792534 None:intergenic
CTTAAACGTGGCCCACTCCG+CGG 0.683812 5.3:+9792440 MS.gene047642:CDS
CCTATCTCCGACGATAGCGA+CGG 0.701116 5.3:+9792713 MS.gene047642:CDS
CCAGGATCGAGAAGAACCGG+CGG 0.705218 5.3:-9792615 None:intergenic
GTAATCGTAGCCGCAACGAG+CGG 0.705311 5.3:-9792495 None:intergenic
CGGCGGAGGAAGTAGCGGTG+AGG 0.714161 5.3:-9792598 None:intergenic
AAATGGTATTCTCTTAAACG+TGG 0.727462 5.3:+9792428 MS.gene047642:CDS
GATGGCGAAGAGTTTGACAG+CGG 0.738106 5.3:+9792871 MS.gene047642:CDS
GTTGTCGTAGCCGCTCGTTG+CGG 0.744946 5.3:+9792485 MS.gene047642:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAATGGTATTCTCTTAAACG+TGG + chr5.3:9792428-9792447 MS.gene047642:CDS 30.0%
GTATGATACTTGAATCTCAA+TGG + chr5.3:9792999-9793018 MS.gene047642:CDS 30.0%
TAAGAGAATACCATTTCTCT+TGG - chr5.3:9792424-9792443 None:intergenic 30.0%
AGAGAGGTTTAGATTAGAGA+TGG + chr5.3:9792961-9792980 MS.gene047642:CDS 35.0%
AGGGTTGGAAAATAAGAAGA+TGG + chr5.3:9792922-9792941 MS.gene047642:CDS 35.0%
GAATGAGGTTAATGGTTTCT+TGG - chr5.3:9792394-9792413 None:intergenic 35.0%
GATTAGAGATGGAGAATAAG+AGG + chr5.3:9792972-9792991 MS.gene047642:CDS 35.0%
TAAGAAGATGGAGATGATGA+AGG + chr5.3:9792934-9792953 MS.gene047642:CDS 35.0%
TCTTCAATGTCGTCATAAGA+TGG + chr5.3:9792538-9792557 MS.gene047642:CDS 35.0%
TGATACTTGAATCTCAATGG+AGG + chr5.3:9793002-9793021 MS.gene047642:CDS 35.0%
! TGATGAAACTGATGATGAAG+AGG + chr5.3:9792841-9792860 MS.gene047642:CDS 35.0%
! TGTTGTTGTCTTGTTACTGT+TGG - chr5.3:9792812-9792831 None:intergenic 35.0%
!! ATTCCATTGGAAAGGCATTT+GGG + chr5.3:9793032-9793051 MS.gene047642:CDS 35.0%
!! GATTGTTGATTCCATTGGAA+AGG + chr5.3:9793024-9793043 MS.gene047642:CDS 35.0%
AGATGATGAAGGAAACAGAG+AGG + chr5.3:9792945-9792964 MS.gene047642:CDS 40.0%
CTTATGACGACATTGAAGAG+CGG - chr5.3:9792537-9792556 None:intergenic 40.0%
GAGGTTAATGGTTTCTTGGT+GGG - chr5.3:9792390-9792409 None:intergenic 40.0%
GTAAGCTCGAATGAGGTTAA+TGG - chr5.3:9792402-9792421 None:intergenic 40.0%
TGAGGTTAATGGTTTCTTGG+TGG - chr5.3:9792391-9792410 None:intergenic 40.0%
TTCGAGCTTACCAAGAGAAA+TGG + chr5.3:9792411-9792430 MS.gene047642:CDS 40.0%
TTGAGGTTTCTTTGTGGGAA+GGG - chr5.3:9792354-9792373 None:intergenic 40.0%
! ATGGGTTGAGGTTTCTTTGT+GGG - chr5.3:9792359-9792378 None:intergenic 40.0%
! GAAACTGATGATGAAGAGGA+GGG + chr5.3:9792845-9792864 MS.gene047642:CDS 40.0%
! TGAAAAAGCATAGTGCTGCT+AGG + chr5.3:9792744-9792763 MS.gene047642:CDS 40.0%
! TGAAACTGATGATGAAGAGG+AGG + chr5.3:9792844-9792863 MS.gene047642:CDS 40.0%
!! GATTCCATTGGAAAGGCATT+TGG + chr5.3:9793031-9793050 MS.gene047642:CDS 40.0%
!! GTCTTGTTACTGTTGGTTTC+TGG - chr5.3:9792805-9792824 None:intergenic 40.0%
!! TGGAGGATTGTTGATTCCAT+TGG + chr5.3:9793019-9793038 MS.gene047642:CDS 40.0%
!!! ATTGAAGAGCGGTTTTGGAA+GGG - chr5.3:9792526-9792545 None:intergenic 40.0%
AGACCCAAATGCCTTTCCAA+TGG - chr5.3:9793038-9793057 None:intergenic 45.0%
AGCTTTGCAGAGAGGATTGT+AGG + chr5.3:9792902-9792921 MS.gene047642:CDS 45.0%
AGTTGAGAAGCTTTGCAGAG+AGG + chr5.3:9792894-9792913 MS.gene047642:CDS 45.0%
CATGAGACGAAAATACTGCC+AGG - chr5.3:9792636-9792655 None:intergenic 45.0%
CTCATGGATGATCTTGAACG+CGG + chr5.3:9792650-9792669 MS.gene047642:CDS 45.0%
GCTTTGCAGAGAGGATTGTA+GGG + chr5.3:9792903-9792922 MS.gene047642:CDS 45.0%
GTTGAGGTTTCTTTGTGGGA+AGG - chr5.3:9792355-9792374 None:intergenic 45.0%
TCTCTTGGTAAGCTCGAATG+AGG - chr5.3:9792409-9792428 None:intergenic 45.0%
! AAGAGTGAGTTCTGGTGAAG+CGG - chr5.3:9792330-9792349 None:intergenic 45.0%
! ACGACATTGAAGAGCGGTTT+TGG - chr5.3:9792531-9792550 None:intergenic 45.0%
! ATAGGGTGCTTCTGAGAAAG+AGG - chr5.3:9792698-9792717 None:intergenic 45.0%
! ATAGTGCTGCTAGGTCTGTT+AGG + chr5.3:9792753-9792772 MS.gene047642:CDS 45.0%
! CTGGCAGTATTTTCGTCTCA+TGG + chr5.3:9792634-9792653 MS.gene047642:CDS 45.0%
!! GATGGGTTGAGGTTTCTTTG+TGG - chr5.3:9792360-9792379 None:intergenic 45.0%
!! TGAGGCGGTGTTTTTCAGAT+CGG - chr5.3:9792583-9792602 None:intergenic 45.0%
!!! CATTGAAGAGCGGTTTTGGA+AGG - chr5.3:9792527-9792546 None:intergenic 45.0%
AGAAACCTCAACCCATCCCA+TGG + chr5.3:9792363-9792382 MS.gene047642:CDS 50.0%
CTGCTAGGTCTGTTAGGTCA+AGG + chr5.3:9792759-9792778 MS.gene047642:CDS 50.0%
GATGGCGAAGAGTTTGACAG+CGG + chr5.3:9792871-9792890 MS.gene047642:CDS 50.0%
GGAAGGGAAAGAGTGAGTTC+TGG - chr5.3:9792338-9792357 None:intergenic 50.0%
TGATGAAGAGGAGGGAGTGA+TGG + chr5.3:9792853-9792872 MS.gene047642:CDS 50.0%
TGCAGAGAGGATTGTAGGGT+TGG + chr5.3:9792907-9792926 MS.gene047642:CDS 50.0%
! ATGGTTTCTTGGTGGGTCCA+TGG - chr5.3:9792383-9792402 None:intergenic 50.0%
! CTTCTGAGAAAGAGGTCGGA+CGG - chr5.3:9792690-9792709 None:intergenic 50.0%
! GGTGCTTCTGAGAAAGAGGT+CGG - chr5.3:9792694-9792713 None:intergenic 50.0%
! TGGTTTCTTGGTGGGTCCAT+GGG - chr5.3:9792382-9792401 None:intergenic 50.0%
! TTTCAGATCGGTGGCGTTGA+CGG - chr5.3:9792571-9792590 None:intergenic 50.0%
CCTATCTCCGACGATAGCGA+CGG + chr5.3:9792713-9792732 MS.gene047642:CDS 55.0%
CGGCAGTCAATGGGAAGAAG+TGG + chr5.3:9792460-9792479 MS.gene047642:CDS 55.0%
CGTCGCTATCGTCGGAGATA+GGG - chr5.3:9792715-9792734 None:intergenic 55.0%
CTGCCAGGATCGAGAAGAAC+CGG - chr5.3:9792621-9792640 None:intergenic 55.0%
GAAAGAGGTCGGACGGAGAT+AGG - chr5.3:9792683-9792702 None:intergenic 55.0%
GGTCGGACGGAGATAGGTAA+AGG - chr5.3:9792677-9792696 None:intergenic 55.0%
GTAATCGTAGCCGCAACGAG+CGG - chr5.3:9792498-9792517 None:intergenic 55.0%
! AGTGAGTTCTGGTGAAGCGG+TGG - chr5.3:9792327-9792346 None:intergenic 55.0%
! CTTCTTCCCATTGACTGCCG+CGG - chr5.3:9792460-9792479 None:intergenic 55.0%
! TCTTGGTGGGTCCATGGGAT+GGG - chr5.3:9792377-9792396 None:intergenic 55.0%
! TTCTTGGTGGGTCCATGGGA+TGG - chr5.3:9792378-9792397 None:intergenic 55.0%
!! GGCGGTGTTTTTCAGATCGG+TGG - chr5.3:9792580-9792599 None:intergenic 55.0%
!!! GAAGAGCGGTTTTGGAAGGG+TGG - chr5.3:9792523-9792542 None:intergenic 55.0%
AGAACCGGCGGAGGAAGTAG+CGG - chr5.3:9792606-9792625 None:intergenic 60.0%
CCAGGATCGAGAAGAACCGG+CGG - chr5.3:9792618-9792637 None:intergenic 60.0%
CCGTCGCTATCGTCGGAGAT+AGG - chr5.3:9792716-9792735 None:intergenic 60.0%
CCTCTCACCGTCGCTATCGT+CGG - chr5.3:9792723-9792742 None:intergenic 60.0%
CGACGATAGCGACGGTGAGA+GGG + chr5.3:9792721-9792740 MS.gene047642:CDS 60.0%
CTTAAACGTGGCCCACTCCG+CGG + chr5.3:9792440-9792459 MS.gene047642:CDS 60.0%
GTTGTCGTAGCCGCTCGTTG+CGG + chr5.3:9792485-9792504 MS.gene047642:CDS 60.0%
!! TGGGTCCATGGGATGGGTTG+AGG - chr5.3:9792371-9792390 None:intergenic 60.0%
CCCACTCCGCGGCAGTCAAT+GGG + chr5.3:9792451-9792470 MS.gene047642:CDS 65.0%
CCGACGATAGCGACGGTGAG+AGG + chr5.3:9792720-9792739 MS.gene047642:CDS 65.0%
CGGAGGAAGTAGCGGTGAGG+CGG - chr5.3:9792598-9792617 None:intergenic 65.0%
CTCACCGCTACTTCCTCCGC+CGG + chr5.3:9792599-9792618 MS.gene047642:CDS 65.0%
GGATCGAGAAGAACCGGCGG+AGG - chr5.3:9792615-9792634 None:intergenic 65.0%
! CCCATTGACTGCCGCGGAGT+GGG - chr5.3:9792454-9792473 None:intergenic 65.0%
! TCCCATTGACTGCCGCGGAG+TGG - chr5.3:9792455-9792474 None:intergenic 65.0%
!! CCGCCGGTTCTTCTCGATCC+TGG + chr5.3:9792615-9792634 MS.gene047642:CDS 65.0%
CGGCGGAGGAAGTAGCGGTG+AGG - chr5.3:9792601-9792620 None:intergenic 70.0%
GCCCACTCCGCGGCAGTCAA+TGG + chr5.3:9792450-9792469 MS.gene047642:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 9792320 9793063 9792320 ID=MS.gene047642
chr5.3 mRNA 9792320 9793063 9792320 ID=MS.gene047642.t1;Parent=MS.gene047642
chr5.3 exon 9792320 9793063 9792320 ID=MS.gene047642.t1.exon1;Parent=MS.gene047642.t1
chr5.3 CDS 9792320 9793063 9792320 ID=cds.MS.gene047642.t1;Parent=MS.gene047642.t1
Gene Sequence

>MS.gene047642

ATGGCCACCGCTTCACCAGAACTCACTCTTTCCCTTCCCACAAAGAAACCTCAACCCATCCCATGGACCCACCAAGAAACCATTAACCTCATTCGAGCTTACCAAGAGAAATGGTATTCTCTTAAACGTGGCCCACTCCGCGGCAGTCAATGGGAAGAAGTGGCTGTTGTCGTAGCCGCTCGTTGCGGCTACGATTACAACCACCCTTCCAAAACCGCTCTTCAATGTCGTCATAAGATGGAAAAACTCCGTCAACGCCACCGATCTGAAAAACACCGCCTCACCGCTACTTCCTCCGCCGGTTCTTCTCGATCCTGGCAGTATTTTCGTCTCATGGATGATCTTGAACGCGGTCCTTTACCTATCTCCGTCCGACCTCTTTCTCAGAAGCACCCTATCTCCGACGATAGCGACGGTGAGAGGGTGAAAAAGCATAGTGCTGCTAGGTCTGTTAGGTCAAGGAGTATTCATACTGTTCTTAACCAGAAACCAACAGTAACAAGACAACAACAACAACAACGTGATGAAACTGATGATGAAGAGGAGGGAGTGATGGCGAAGAGTTTGACAGCGGAGTTGAGAAGCTTTGCAGAGAGGATTGTAGGGTTGGAAAATAAGAAGATGGAGATGATGAAGGAAACAGAGAGGTTTAGATTAGAGATGGAGAATAAGAGGATTCGTATGATACTTGAATCTCAATGGAGGATTGTTGATTCCATTGGAAAGGCATTTGGGTCTTCTTAG
Protein sequence

>MS.gene047642.t1

MATASPELTLSLPTKKPQPIPWTHQETINLIRAYQEKWYSLKRGPLRGSQWEEVAVVVAARCGYDYNHPSKTALQCRHKMEKLRQRHRSEKHRLTATSSAGSSRSWQYFRLMDDLERGPLPISVRPLSQKHPISDDSDGERVKKHSAARSVRSRSIHTVLNQKPTVTRQQQQQRDETDDEEEGVMAKSLTAELRSFAERIVGLENKKMEMMKETERFRLEMENKRIRMILESQWRIVDSIGKAFGSS