Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048043.t1 | KEH35336.1 | 62.6 | 235 | 27 | 2 | 2 | 175 | 13 | 247 | 3.20E-67 | 264.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048043.t1 | Q8LAV5 | 27.5 | 102 | 72 | 1 | 1 | 102 | 1 | 100 | 2.1e-06 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048043.t1 | A0A072V0X2 | 62.6 | 235 | 27 | 2 | 2 | 175 | 13 | 247 | 2.3e-67 | 264.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene048043.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048043.t1 | MTR_3g088515 | 62.553 | 235 | 27 | 2 | 2 | 175 | 13 | 247 | 2.01e-89 | 261 |
| MS.gene048043.t1 | MTR_1g046340 | 52.609 | 230 | 54 | 3 | 1 | 175 | 1 | 230 | 3.45e-71 | 218 |
| MS.gene048043.t1 | MTR_1g046200 | 47.727 | 220 | 64 | 3 | 5 | 175 | 2 | 219 | 4.44e-57 | 182 |
| MS.gene048043.t1 | MTR_1g046320 | 71.560 | 109 | 31 | 0 | 1 | 109 | 1 | 109 | 1.99e-47 | 157 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATCATCTTCAAAGTTT+TGG | 0.226384 | 3.3:+73991957 | None:intergenic |
| GCATAGAAGTATGGATTTCC+TGG | 0.326303 | 3.3:+73991911 | None:intergenic |
| CAGAGAAGAAAATTCATTAG+AGG | 0.353395 | 3.3:-73992481 | MS.gene048043:CDS |
| ATGAGCTTCACAAAAGCAAT+TGG | 0.382357 | 3.3:+73992624 | None:intergenic |
| CGCGTCAAAGCACATTCAAT+TGG | 0.401347 | 3.3:-73992540 | MS.gene048043:CDS |
| ACATTACAGACCCAACGAAT+TGG | 0.430909 | 3.3:-73991885 | None:intergenic |
| GATTGTGGTTTAGGGAGCAT+TGG | 0.466163 | 3.3:-73991996 | MS.gene048043:intron |
| AAGAACTAAACCAATTCGTT+GGG | 0.487807 | 3.3:+73991875 | None:intergenic |
| ATGAAAGCAGAAAAGAAGAA+AGG | 0.488071 | 3.3:-73992168 | MS.gene048043:intron |
| AAAGAACTAAACCAATTCGT+TGG | 0.488811 | 3.3:+73991874 | None:intergenic |
| CTCTTCCTCTATGATCCCTT+AGG | 0.492258 | 3.3:+73992568 | None:intergenic |
| TCTCATAATGCTTCTCTATG+AGG | 0.504327 | 3.3:+73992840 | None:intergenic |
| GGGATCATAGAGGAAGAGAT+TGG | 0.510517 | 3.3:-73992563 | MS.gene048043:CDS |
| AAGCACATTCAATTGGTGGC+AGG | 0.516729 | 3.3:-73992533 | MS.gene048043:CDS |
| AAGAAGAAAGGAGTTGACAA+GGG | 0.522584 | 3.3:-73992156 | MS.gene048043:CDS |
| AGAGAAGCATTATGAGAGAA+TGG | 0.527328 | 3.3:-73992834 | MS.gene048043:intron |
| GTTGAAATGTATATTCAGCC+AGG | 0.532204 | 3.3:-73991929 | MS.gene048043:CDS |
| AAAGAAGAAAGGAGTTGACA+AGG | 0.542225 | 3.3:-73992157 | MS.gene048043:CDS |
| TGAGCTTCACAAAAGCAATT+GGG | 0.545679 | 3.3:+73992625 | None:intergenic |
| GTCAAAGCACATTCAATTGG+TGG | 0.546694 | 3.3:-73992537 | MS.gene048043:CDS |
| TTTCAATATGTGTGAAAACA+AGG | 0.554136 | 3.3:+73992448 | None:intergenic |
| GAGGAACACCTTGGCAGTGT+TGG | 0.557953 | 3.3:+73992880 | None:intergenic |
| AGCTCATGCTTTCGAAACAA+AGG | 0.561664 | 3.3:-73992608 | MS.gene048043:CDS |
| GGAAGACTTTGAAGAAGTTG+AGG | 0.564880 | 3.3:+73992861 | None:intergenic |
| GCTCATGCTTTCGAAACAAA+GGG | 0.566004 | 3.3:-73992607 | MS.gene048043:CDS |
| GAAGAAGTTGAGGAACACCT+TGG | 0.598409 | 3.3:+73992871 | None:intergenic |
| TTTATCCTAAGGGATCATAG+AGG | 0.616153 | 3.3:-73992573 | MS.gene048043:CDS |
| AATTATGACCAACACTGCCA+AGG | 0.625272 | 3.3:-73992888 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAGTTTTTTTTTTTTTTTT+TGG | + | chr3.3:73992065-73992084 | None:intergenic | 10.0% |
| !! | AAACTTAATAAAATAATCGG+AGG | + | chr3.3:73991987-73992006 | None:intergenic | 20.0% |
| !!! | ATTGCAATATTTTATCCTAA+GGG | - | chr3.3:73992187-73992206 | MS.gene048043:intron | 20.0% |
| !!! | TATTGCAATATTTTATCCTA+AGG | - | chr3.3:73992186-73992205 | MS.gene048043:intron | 20.0% |
| ! | ATCAATGTGTAAAAAGACAA+AGG | - | chr3.3:73992379-73992398 | MS.gene048043:intron | 25.0% |
| ! | ATTTACCTAATGATTGATTG+TGG | - | chr3.3:73992759-73992778 | MS.gene048043:intron | 25.0% |
| ! | GGAAAATAATAATATGTTGG+AGG | - | chr3.3:73992415-73992434 | MS.gene048043:CDS | 25.0% |
| ! | GGAGGAAAATAATAATATGT+TGG | - | chr3.3:73992412-73992431 | MS.gene048043:CDS | 25.0% |
| ! | TAATGATTGATTGTGGTTTA+GGG | - | chr3.3:73992766-73992785 | MS.gene048043:intron | 25.0% |
| ! | TTTCAATATGTGTGAAAACA+AGG | + | chr3.3:73992325-73992344 | None:intergenic | 25.0% |
| !! | TAATGTTTTGCATAGAAGTA+TGG | + | chr3.3:73992871-73992890 | None:intergenic | 25.0% |
| !! | TTTCATCATCTTCAAAGTTT+TGG | + | chr3.3:73992816-73992835 | None:intergenic | 25.0% |
| ATGAAAGCAGAAAAGAAGAA+AGG | - | chr3.3:73992602-73992621 | MS.gene048043:CDS | 30.0% | |
| CAAAAACATACATGCTTTGA+AGG | + | chr3.3:73992675-73992694 | None:intergenic | 30.0% | |
| CAGAGAAGAAAATTCATTAG+AGG | - | chr3.3:73992289-73992308 | MS.gene048043:intron | 30.0% | |
| CTAAACCACAATCAATCATT+AGG | + | chr3.3:73992767-73992786 | None:intergenic | 30.0% | |
| CTAATGATTGATTGTGGTTT+AGG | - | chr3.3:73992765-73992784 | MS.gene048043:intron | 30.0% | |
| GTGTAAAAAGACAAAGGTAA+TGG | - | chr3.3:73992385-73992404 | MS.gene048043:intron | 30.0% | |
| TAATAATATGTTGGAGGATG+AGG | - | chr3.3:73992421-73992440 | MS.gene048043:CDS | 30.0% | |
| ! | AGATCAATGTTACTTAAGAG+TGG | + | chr3.3:73992093-73992112 | None:intergenic | 30.0% |
| AAAGAAGAAAGGAGTTGACA+AGG | - | chr3.3:73992613-73992632 | MS.gene048043:CDS | 35.0% | |
| AAGAAGAAAGGAGTTGACAA+GGG | - | chr3.3:73992614-73992633 | MS.gene048043:CDS | 35.0% | |
| ATGAGCTTCACAAAAGCAAT+TGG | + | chr3.3:73992149-73992168 | None:intergenic | 35.0% | |
| GTTGAAATGTATATTCAGCC+AGG | - | chr3.3:73992841-73992860 | MS.gene048043:CDS | 35.0% | |
| TCTCATAATGCTTCTCTATG+AGG | + | chr3.3:73991933-73991952 | None:intergenic | 35.0% | |
| TGAGCTTCACAAAAGCAATT+GGG | + | chr3.3:73992148-73992167 | None:intergenic | 35.0% | |
| TTTATCCTAAGGGATCATAG+AGG | - | chr3.3:73992197-73992216 | MS.gene048043:intron | 35.0% | |
| !! | AAAGACAAAGGTAATGGATG+AGG | - | chr3.3:73992391-73992410 | MS.gene048043:intron | 35.0% |
| !! | AGAGAAGCATTATGAGAGAA+TGG | - | chr3.3:73991936-73991955 | MS.gene048043:CDS | 35.0% |
| !!! | GGCTTTATTTTGATGATGGT+TGG | - | chr3.3:73992258-73992277 | MS.gene048043:intron | 35.0% |
| AATGGTAGAGGAAGAAGAAG+AGG | - | chr3.3:73992496-73992515 | MS.gene048043:CDS | 40.0% | |
| AGCTCATGCTTTCGAAACAA+AGG | - | chr3.3:73992162-73992181 | MS.gene048043:intron | 40.0% | |
| GAAGAAGATGAAGATGGTGA+TGG | - | chr3.3:73992518-73992537 | MS.gene048043:CDS | 40.0% | |
| GAAGAGGAAGAAGATGAAGA+TGG | - | chr3.3:73992512-73992531 | MS.gene048043:CDS | 40.0% | |
| GCTCATGCTTTCGAAACAAA+GGG | - | chr3.3:73992163-73992182 | MS.gene048043:intron | 40.0% | |
| GTCAAAGCACATTCAATTGG+TGG | - | chr3.3:73992233-73992252 | MS.gene048043:intron | 40.0% | |
| ! | GCATAGAAGTATGGATTTCC+TGG | + | chr3.3:73992862-73992881 | None:intergenic | 40.0% |
| ! | GGAAGACTTTGAAGAAGTTG+AGG | + | chr3.3:73991912-73991931 | None:intergenic | 40.0% |
| ! | TGATGATGAAGACGATGATG+AGG | - | chr3.3:73992541-73992560 | MS.gene048043:CDS | 40.0% |
| !!! | GGCAGGCTTTATTTTGATGA+TGG | - | chr3.3:73992254-73992273 | MS.gene048043:intron | 40.0% |
| CGACGGTTATACAATGGTAG+AGG | - | chr3.3:73992484-73992503 | MS.gene048043:CDS | 45.0% | |
| CGCGTCAAAGCACATTCAAT+TGG | - | chr3.3:73992230-73992249 | MS.gene048043:intron | 45.0% | |
| CTCTTCCTCTATGATCCCTT+AGG | + | chr3.3:73992205-73992224 | None:intergenic | 45.0% | |
| GAAGAAGTTGAGGAACACCT+TGG | + | chr3.3:73991902-73991921 | None:intergenic | 45.0% | |
| GGGATCATAGAGGAAGAGAT+TGG | - | chr3.3:73992207-73992226 | MS.gene048043:intron | 45.0% | |
| TATGTTGGAGGATGAGGAAG+AGG | - | chr3.3:73992427-73992446 | MS.gene048043:CDS | 45.0% | |
| TGAAGACGATGATGAGGATG+AGG | - | chr3.3:73992547-73992566 | MS.gene048043:CDS | 45.0% | |
| TGACGACGACGGTTATACAA+TGG | - | chr3.3:73992478-73992497 | MS.gene048043:CDS | 45.0% | |
| ! | AAGCACATTCAATTGGTGGC+AGG | - | chr3.3:73992237-73992256 | MS.gene048043:intron | 45.0% |
| ! | GAAGAGGAAGGTGATGATGA+TGG | - | chr3.3:73992443-73992462 | MS.gene048043:CDS | 45.0% |
| ! | GATTGTGGTTTAGGGAGCAT+TGG | - | chr3.3:73992774-73992793 | MS.gene048043:intron | 45.0% |
| !! | GACAAAGGTAATGGATGAGG+AGG | - | chr3.3:73992394-73992413 | MS.gene048043:CDS | 45.0% |
| !! | AAAAAACTTAATAAAATAAT+CGG | + | chr3.3:73991990-73992009 | None:intergenic | 5.0% |
| CGATGATGAGGATGAGGATG+AGG | - | chr3.3:73992553-73992572 | MS.gene048043:CDS | 50.0% | |
| TTGGAGGATGAGGAAGAGGA+AGG | - | chr3.3:73992431-73992450 | MS.gene048043:CDS | 50.0% | |
| ! | GGAGATGATGATGACGACGA+CGG | - | chr3.3:73992467-73992486 | MS.gene048043:CDS | 50.0% |
| !! | GAGGAAGGTGATGATGATGG+TGG | - | chr3.3:73992446-73992465 | MS.gene048043:CDS | 50.0% |
| GAGGAACACCTTGGCAGTGT+TGG | + | chr3.3:73991893-73991912 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73991886 | 73992906 | 73991886 | ID=MS.gene048043 |
| chr3.3 | mRNA | 73991886 | 73992906 | 73991886 | ID=MS.gene048043.t1;Parent=MS.gene048043 |
| chr3.3 | exon | 73992835 | 73992906 | 73992835 | ID=MS.gene048043.t1.exon1;Parent=MS.gene048043.t1 |
| chr3.3 | CDS | 73992835 | 73992906 | 73992835 | ID=cds.MS.gene048043.t1;Parent=MS.gene048043.t1 |
| chr3.3 | exon | 73992392 | 73992652 | 73992392 | ID=MS.gene048043.t1.exon2;Parent=MS.gene048043.t1 |
| chr3.3 | CDS | 73992392 | 73992652 | 73992392 | ID=cds.MS.gene048043.t1;Parent=MS.gene048043.t1 |
| chr3.3 | exon | 73992109 | 73992180 | 73992109 | ID=MS.gene048043.t1.exon3;Parent=MS.gene048043.t1 |
| chr3.3 | CDS | 73992109 | 73992180 | 73992109 | ID=cds.MS.gene048043.t1;Parent=MS.gene048043.t1 |
| chr3.3 | exon | 73991886 | 73992005 | 73991886 | ID=MS.gene048043.t1.exon4;Parent=MS.gene048043.t1 |
| chr3.3 | CDS | 73991886 | 73992005 | 73991886 | ID=cds.MS.gene048043.t1;Parent=MS.gene048043.t1 |
| Gene Sequence |
| Protein sequence |
