AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048143

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048143.t1 MTR_5g082910 89.610 77 6 1 96 172 3 77 5.15e-42 143
MS.gene048143.t1 MTR_5g016510 65.574 61 21 0 111 171 2 62 6.69e-24 96.3
MS.gene048143.t1 MTR_4g094982 63.934 61 22 0 111 171 2 62 5.11e-23 93.2
MS.gene048143.t1 MTR_3g101290 63.333 60 22 0 111 170 34 93 1.47e-22 90.9
MS.gene048143.t1 MTR_6g015455 55.128 78 29 2 111 184 2 77 2.53e-21 91.3
MS.gene048143.t1 MTR_4g019370 62.712 59 22 0 112 170 36 94 9.83e-20 85.5
MS.gene048143.t1 MTR_3g089045 50.877 57 28 0 114 170 16 72 4.61e-13 66.2
MS.gene048143.t1 MTR_6g027370 38.462 91 53 1 80 170 68 155 5.53e-13 66.6
MS.gene048143.t1 MTR_5g010650 46.774 62 33 0 109 170 105 166 6.25e-13 66.6
MS.gene048143.t1 MTR_6g027340 40.580 69 41 0 102 170 4 72 6.73e-13 64.7
MS.gene048143.t1 MTR_6g027360 40.580 69 41 0 102 170 66 134 1.79e-12 64.7
MS.gene048143.t1 MTR_6g074860 49.123 57 29 0 114 170 4 60 2.45e-12 63.9
MS.gene048143.t1 MTR_3g110028 48.214 56 29 0 109 164 80 135 4.04e-12 64.7
MS.gene048143.t1 MTR_5g041570 41.333 75 40 1 100 170 116 190 1.13e-11 63.2
MS.gene048143.t1 MTR_5g088640 50.820 61 29 1 111 170 11 71 1.32e-11 62.0
MS.gene048143.t1 MTR_5g088080 50.820 61 29 1 111 170 11 71 1.59e-11 62.0
MS.gene048143.t1 MTR_5g088150 50.820 61 29 1 111 170 11 71 1.59e-11 62.0
MS.gene048143.t1 MTR_5g488210 50.820 61 29 1 111 170 11 71 1.59e-11 62.0
MS.gene048143.t1 MTR_5g488170 50.820 61 29 1 111 170 11 71 1.74e-11 61.6
MS.gene048143.t1 MTR_5g062790 50.820 61 29 1 111 170 8 68 2.19e-11 61.6
MS.gene048143.t1 MTR_1g026870 44.643 56 31 0 109 164 80 135 3.34e-11 62.0
MS.gene048143.t1 MTR_3g077650 47.170 53 28 0 113 165 180 232 4.95e-11 61.2
MS.gene048143.t1 MTR_4g082040 40.506 79 45 1 94 170 83 161 8.20e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048143.t1 AT3G50060 62.903 62 23 0 110 171 2 63 2.25e-23 94.7
MS.gene048143.t1 AT5G67300 63.333 60 22 0 111 170 3 62 3.81e-22 91.7
MS.gene048143.t1 AT2G39880 54.054 74 30 1 97 170 37 106 9.56e-21 88.6
MS.gene048143.t1 AT2G23290 61.667 60 23 0 111 170 10 69 1.36e-20 87.4
MS.gene048143.t1 AT4G37260 57.143 63 27 0 108 170 7 69 2.32e-20 87.0
MS.gene048143.t1 AT3G55730 48.864 88 45 0 83 170 25 112 1.07e-19 85.9
MS.gene048143.t1 AT3G09230 61.667 60 23 0 111 170 52 111 1.02e-18 83.2
MS.gene048143.t1 AT1G73410 50.877 57 28 0 114 170 6 62 1.90e-13 67.0
MS.gene048143.t1 AT1G73410 50.877 57 28 0 114 170 6 62 1.92e-13 66.2
MS.gene048143.t1 AT1G17950 49.123 57 29 0 114 170 5 61 2.66e-12 63.9
MS.gene048143.t1 AT4G33450 47.368 57 30 0 114 170 19 75 4.01e-12 63.5
MS.gene048143.t1 AT5G11510 44.643 56 31 0 109 164 76 131 1.32e-11 63.2
MS.gene048143.t1 AT5G11510 44.643 56 31 0 109 164 76 131 1.32e-11 63.2
MS.gene048143.t1 AT5G11510 44.643 56 31 0 109 164 76 131 1.32e-11 63.2
MS.gene048143.t1 AT5G11510 44.643 56 31 0 109 164 76 131 1.39e-11 63.2
MS.gene048143.t1 AT5G11510 44.643 56 31 0 109 164 76 131 1.44e-11 62.8
MS.gene048143.t1 AT5G17800 49.123 57 29 0 114 170 93 149 1.58e-11 62.4
MS.gene048143.t1 AT3G09370 44.643 56 31 0 109 164 138 193 1.96e-11 62.4
MS.gene048143.t1 AT5G02320 43.548 62 35 0 109 170 62 123 2.15e-11 62.4
MS.gene048143.t1 AT5G02320 43.548 62 35 0 109 170 122 183 2.23e-11 62.4
MS.gene048143.t1 AT5G02320 43.548 62 35 0 109 170 122 183 2.23e-11 62.4
MS.gene048143.t1 AT3G09370 44.643 56 31 0 109 164 130 185 2.29e-11 62.4
MS.gene048143.t1 AT4G32730 48.214 56 29 0 109 164 82 137 2.35e-11 62.4
MS.gene048143.t1 AT4G32730 48.214 56 29 0 109 164 82 137 2.35e-11 62.4
MS.gene048143.t1 AT4G32730 48.214 56 29 0 109 164 82 137 2.35e-11 62.4
MS.gene048143.t1 AT4G32730 48.214 56 29 0 109 164 82 137 2.35e-11 62.4
MS.gene048143.t1 AT5G11050 45.283 53 29 0 113 165 104 156 2.39e-11 62.0
MS.gene048143.t1 AT3G09370 44.643 56 31 0 109 164 125 180 2.39e-11 62.0
MS.gene048143.t1 AT4G32730 48.214 56 29 0 109 164 82 137 2.68e-11 62.0
MS.gene048143.t1 AT5G58850 41.509 53 31 0 113 165 104 156 7.83e-11 60.5

Find 37 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGATTTCAGCAACGTTTGTT+TGG 0.210949 4.4:-80816503 MS.gene048143:CDS
CGTTTGTTGTTGGTTTCGTT+TGG 0.329695 4.4:+80816547 None:intergenic
GCCACCACCGCCGGTGTAAA+AGG 0.337215 4.4:+80816070 None:intergenic
GATTTCAGCAACGTTTGTTT+GGG 0.369346 4.4:-80816502 MS.gene048143:CDS
TTCAAGAAATCGAAGGATCT+TGG 0.379992 4.4:+80816011 None:intergenic
AACGGAGTCCCCGATCGTTC+TGG 0.385664 4.4:-80816148 MS.gene048143:CDS
ACCACCGTTTGAATTCGAAT+TGG 0.391041 4.4:+80816282 None:intergenic
GTGATAAAGTGAAGAGTTCA+TGG 0.411815 4.4:-80816243 MS.gene048143:CDS
AGGAAAACGGTGCAAGAAAC+TGG 0.414736 4.4:-80816183 MS.gene048143:CDS
AGATGAAACATTGGTGAAAC+TGG 0.432466 4.4:-80816209 MS.gene048143:CDS
ACGGAGTCCCCGATCGTTCT+GGG 0.435316 4.4:-80816147 MS.gene048143:CDS
CAGGACTTCCCAGAACGATC+GGG 0.465466 4.4:+80816139 None:intergenic
GTATCGGAATCGTTTGTTGT+TGG 0.495808 4.4:+80816537 None:intergenic
ATTACACCACCGCAGACGGC+AGG 0.523815 4.4:+80816120 None:intergenic
GGAAGTCCTGCCGTCTGCGG+TGG 0.531786 4.4:-80816126 MS.gene048143:CDS
TGTCTTTCAAATTCTTGTTG+AGG 0.532596 4.4:-80817735 MS.gene048143:intron
GCAGGACTTCCCAGAACGAT+CGG 0.533846 4.4:+80816138 None:intergenic
AGTTCCAATTCGAATTCAAA+CGG 0.535816 4.4:-80816286 MS.gene048143:CDS
AATCGATCGCAGAAGCGTAT+CGG 0.545693 4.4:+80816521 None:intergenic
GATCGTTGTGCCACCACCGC+CGG 0.546241 4.4:+80816061 None:intergenic
TCGAATCTGAGTTGCACGCA+AGG 0.556208 4.4:-80816478 MS.gene048143:CDS
CTGGGAAGTCCTGCCGTCTG+CGG 0.559594 4.4:-80816129 MS.gene048143:CDS
ATTTCTAGCGAACGATGCGT+CGG 0.565476 4.4:-80816421 MS.gene048143:CDS
AAAGGAAGGTGTTGAACGGT+TGG 0.567448 4.4:+80816088 None:intergenic
AATTCGAATTCAAACGGTGG+TGG 0.570811 4.4:-80816280 MS.gene048143:CDS
CCACCGCCGGTGTAAAAGGA+AGG 0.581661 4.4:+80816074 None:intergenic
AACTGGTCTGTGATAAGCAA+CGG 0.591578 4.4:-80816166 MS.gene048143:CDS
GTGAAACTGGTGAAGGAAAA+CGG 0.592557 4.4:-80816196 MS.gene048143:CDS
GCTGATTACACCACCGCAGA+CGG 0.606009 4.4:+80816116 None:intergenic
CTCCCATTTCAAGAAATCGA+AGG 0.620168 4.4:+80816004 None:intergenic
TGTAAAAGGAAGGTGTTGAA+CGG 0.650810 4.4:+80816084 None:intergenic
AAGAACAGAAACAAACACGT+TGG 0.651382 4.4:-80816379 MS.gene048143:CDS
AGGACTTCCCAGAACGATCG+GGG 0.651493 4.4:+80816140 None:intergenic
GGTGGTGGTGTTGATGAAAA+CGG 0.651690 4.4:-80816265 MS.gene048143:CDS
AACATTGGTGAAACTGGTGA+AGG 0.661429 4.4:-80816203 MS.gene048143:CDS
CTAGAAATCTCAGTTCTGCG+TGG 0.668673 4.4:+80816436 None:intergenic
TCCAATTCGAATTCAAACGG+TGG 0.678610 4.4:-80816283 MS.gene048143:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGATTGAAAAATAAAAAT+TGG + chr4.4:80816848-80816867 None:intergenic 10.0%
!!! TTTAGTTTTAATTTTCATTT+TGG - chr4.4:80816645-80816664 MS.gene048143:intron 10.0%
!! TAAGATTAATCATTTGAATA+TGG - chr4.4:80816055-80816074 MS.gene048143:CDS 15.0%
!!! TTTAAAATGGATTTTTAACA+AGG + chr4.4:80816478-80816497 None:intergenic 15.0%
!! AAAAAGAATTGATGAAATGA+AGG + chr4.4:80817128-80817147 None:intergenic 20.0%
!! AAAATCGATTGTGAATTATT+GGG + chr4.4:80817100-80817119 None:intergenic 20.0%
!! ATTAATCATTTGAATATGGT+TGG - chr4.4:80816059-80816078 MS.gene048143:CDS 20.0%
!! TTTGCAAAATTCATGAAAAA+GGG - chr4.4:80816200-80816219 MS.gene048143:CDS 20.0%
!!! ACTCAGATGAATTTTTAAAA+TGG + chr4.4:80816491-80816510 None:intergenic 20.0%
!!! TATTTTTAGGTCCAAAATTA+GGG + chr4.4:80816092-80816111 None:intergenic 20.0%
!!! TTCAAATGCAAATTTTGAAT+TGG + chr4.4:80816331-80816350 None:intergenic 20.0%
! ACTTATTCAAACTACTTGAA+AGG + chr4.4:80816284-80816303 None:intergenic 25.0%
! CAAAATCGATTGTGAATTAT+TGG + chr4.4:80817101-80817120 None:intergenic 25.0%
! GTTTGCAAAATTCATGAAAA+AGG - chr4.4:80816199-80816218 MS.gene048143:CDS 25.0%
! TAAAAACGAAGATGAAACAT+TGG - chr4.4:80817530-80817549 MS.gene048143:intron 25.0%
!! AAAAGATTCAGAGACAATTT+TGG + chr4.4:80816424-80816443 None:intergenic 25.0%
!! AAAGATTCAGAGACAATTTT+GGG + chr4.4:80816423-80816442 None:intergenic 25.0%
!! ACACAACAAAAACAGTTTTA+AGG - chr4.4:80817410-80817429 MS.gene048143:intron 25.0%
!! AGAGTTGAGTTAGTATTTTT+AGG + chr4.4:80816105-80816124 None:intergenic 25.0%
!! GACTCTAACAAACTTTAAAA+AGG + chr4.4:80816701-80816720 None:intergenic 25.0%
!!! GTATTTTTAGGTCCAAAATT+AGG + chr4.4:80816093-80816112 None:intergenic 25.0%
ACCAACTTTGACCTATTTAT+AGG + chr4.4:80816793-80816812 None:intergenic 30.0%
ACCTATAAATAGGTCAAAGT+TGG - chr4.4:80816789-80816808 MS.gene048143:intron 30.0%
AGTTCCAATTCGAATTCAAA+CGG - chr4.4:80817462-80817481 MS.gene048143:intron 30.0%
TGTCTTTCAAATTCTTGTTG+AGG - chr4.4:80816013-80816032 MS.gene048143:CDS 30.0%
TTGAAGATGAAGTTAGTTAG+AGG + chr4.4:80816920-80816939 None:intergenic 30.0%
! ATGCAAATAACAAAAGTACG+AGG + chr4.4:80816672-80816691 None:intergenic 30.0%
!! ACTGAAAGAAATTTTGACAC+AGG + chr4.4:80816240-80816259 None:intergenic 30.0%
!!! CAATTCTTTTTTCCGTTCTT+CGG - chr4.4:80817137-80817156 MS.gene048143:intron 30.0%
!!! GTTTTAAGGTAGAAGAAAAG+AGG - chr4.4:80817424-80817443 MS.gene048143:intron 30.0%
!!! TTGGTGTTATACCCTAATTT+TGG - chr4.4:80816078-80816097 MS.gene048143:CDS 30.0%
AAGAACAGAAACAAACACGT+TGG - chr4.4:80817369-80817388 MS.gene048143:intron 35.0%
AGATGAAACATTGGTGAAAC+TGG - chr4.4:80817539-80817558 MS.gene048143:intron 35.0%
CAATAGTGATGTTGAGAGAT+TGG + chr4.4:80817173-80817192 None:intergenic 35.0%
GACCTATTTATAGGTGATGT+GGG + chr4.4:80816784-80816803 None:intergenic 35.0%
GATTTCAGCAACGTTTGTTT+GGG - chr4.4:80817246-80817265 MS.gene048143:intron 35.0%
GTGATAAAGTGAAGAGTTCA+TGG - chr4.4:80817505-80817524 MS.gene048143:intron 35.0%
TCGATTGTGAATTATTGGGT+TGG + chr4.4:80817096-80817115 None:intergenic 35.0%
TCTTGTTGAGGTTGAAGTTT+GGG - chr4.4:80816025-80816044 MS.gene048143:CDS 35.0%
TGACCTATTTATAGGTGATG+TGG + chr4.4:80816785-80816804 None:intergenic 35.0%
TTCCCACATCACCTATAAAT+AGG - chr4.4:80816779-80816798 MS.gene048143:intron 35.0%
TTCTTGTTGAGGTTGAAGTT+TGG - chr4.4:80816024-80816043 MS.gene048143:CDS 35.0%
! AAATAGGTCAAAGTTGGTCA+AGG - chr4.4:80816795-80816814 MS.gene048143:intron 35.0%
! GTTTGTTAGAGTCAAAGTCA+AGG - chr4.4:80816708-80816727 MS.gene048143:intron 35.0%
! TTGGTTATGGAGGTTAGTTA+TGG + chr4.4:80816997-80817016 None:intergenic 35.0%
!! GTGAATTATTGGGTTGGATT+TGG + chr4.4:80817090-80817109 None:intergenic 35.0%
!! TGTAAAAGGAAGGTGTTGAA+CGG + chr4.4:80817667-80817686 None:intergenic 35.0%
!! TTATTGGGTTGGATTTGGTT+AGG + chr4.4:80817085-80817104 None:intergenic 35.0%
AAAAGACCAAAATGCCCCTA+GGG + chr4.4:80816375-80816394 None:intergenic 40.0%
AACATTGGTGAAACTGGTGA+AGG - chr4.4:80817545-80817564 MS.gene048143:intron 40.0%
AATTCGAATTCAAACGGTGG+TGG - chr4.4:80817468-80817487 MS.gene048143:intron 40.0%
ACCAATGAAACAATGCCATG+TGG + chr4.4:80816752-80816771 None:intergenic 40.0%
ACCACCGTTTGAATTCGAAT+TGG + chr4.4:80817469-80817488 None:intergenic 40.0%
AGTTAGAGGAGAGAGAAAGT+GGG + chr4.4:80816906-80816925 None:intergenic 40.0%
CGATTTCAGCAACGTTTGTT+TGG - chr4.4:80817245-80817264 MS.gene048143:intron 40.0%
CTTGTTGAGGTTGAAGTTTG+GGG - chr4.4:80816026-80816045 MS.gene048143:CDS 40.0%
GGAATGTTTGAACCGAAGAA+CGG + chr4.4:80817152-80817171 None:intergenic 40.0%
GTGAAACTGGTGAAGGAAAA+CGG - chr4.4:80817552-80817571 MS.gene048143:intron 40.0%
TAGTTAGAGGAGAGAGAAAG+TGG + chr4.4:80816907-80816926 None:intergenic 40.0%
TCCAATTCGAATTCAAACGG+TGG - chr4.4:80817465-80817484 MS.gene048143:intron 40.0%
! AACTGGTCTGTGATAAGCAA+CGG - chr4.4:80817582-80817601 MS.gene048143:intron 40.0%
! GGTTGGATTTGGTTAGGAAT+TGG + chr4.4:80817079-80817098 None:intergenic 40.0%
! GTTATGGAGGTTAGTTATGG+TGG + chr4.4:80816994-80817013 None:intergenic 40.0%
! TCAAAGTTGGTCAAGGTTCA+TGG - chr4.4:80816802-80816821 MS.gene048143:intron 40.0%
! TCAACACCTTCCTTTTACAC+CGG - chr4.4:80817668-80817687 MS.gene048143:intron 40.0%
! TGGATTTGGTTAGGAATTGG+AGG + chr4.4:80817076-80817095 None:intergenic 40.0%
!! CGTTTGTTGTTGGTTTCGTT+TGG + chr4.4:80817204-80817223 None:intergenic 40.0%
!! GTATCGGAATCGTTTGTTGT+TGG + chr4.4:80817214-80817233 None:intergenic 40.0%
!! GTTGTGGAAGTTGATGAAGA+TGG + chr4.4:80817042-80817061 None:intergenic 40.0%
!! TTAGTCGTTTTAAGCCTCCA+GGG - chr4.4:80816516-80816535 MS.gene048143:CDS 40.0%
!! TTTAGTCGTTTTAAGCCTCC+AGG - chr4.4:80816515-80816534 MS.gene048143:CDS 40.0%
AAAGACCAAAATGCCCCTAG+GGG + chr4.4:80816374-80816393 None:intergenic 45.0%
AGGAAAACGGTGCAAGAAAC+TGG - chr4.4:80817565-80817584 MS.gene048143:intron 45.0%
AGGTTTGTGGTGGTTGTTAG+AGG + chr4.4:80816943-80816962 None:intergenic 45.0%
ATAGGTGATGTGGGAATGCT+TGG + chr4.4:80816775-80816794 None:intergenic 45.0%
ATGGTGGTTATGGAGGTTTG+TGG + chr4.4:80816956-80816975 None:intergenic 45.0%
CTAGAAATCTCAGTTCTGCG+TGG + chr4.4:80817315-80817334 None:intergenic 45.0%
GAAAAGACCAAAATGCCCCT+AGG + chr4.4:80816376-80816395 None:intergenic 45.0%
GCCACATGGCATTGTTTCAT+TGG - chr4.4:80816748-80816767 MS.gene048143:intron 45.0%
GGAATTGGAGGCTGTTACAA+TGG + chr4.4:80817064-80817083 None:intergenic 45.0%
GTGGTGGTTTATGGTGGTTA+TGG + chr4.4:80816966-80816985 None:intergenic 45.0%
GTGGTTTATGGTGGTTATGG+AGG + chr4.4:80816963-80816982 None:intergenic 45.0%
GTTACAAGTCAAGTGCCACA+TGG - chr4.4:80816734-80816753 MS.gene048143:intron 45.0%
GTTAGAGGAGAGAGAAAGTG+GGG + chr4.4:80816905-80816924 None:intergenic 45.0%
TGAAATGACCAAAATGCCCC+TGG + chr4.4:80816536-80816555 None:intergenic 45.0%
TTAGAGGAGAGAGAAAGTGG+GGG + chr4.4:80816904-80816923 None:intergenic 45.0%
! AATCGATCGCAGAAGCGTAT+CGG + chr4.4:80817230-80817249 None:intergenic 45.0%
! ATGGAGGTTAGTTATGGTGG+TGG + chr4.4:80816991-80817010 None:intergenic 45.0%
! GTGGTGGAAATTGGTTATGG+AGG + chr4.4:80817007-80817026 None:intergenic 45.0%
! TTGAGTCAGTTTTGCCCCTA+GGG - chr4.4:80816357-80816376 MS.gene048143:CDS 45.0%
! TTTGAGTCAGTTTTGCCCCT+AGG - chr4.4:80816356-80816375 MS.gene048143:CDS 45.0%
!! AAAGGAAGGTGTTGAACGGT+TGG + chr4.4:80817663-80817682 None:intergenic 45.0%
!! ATGAAGATGGTGGTGGTTTG+TGG + chr4.4:80817029-80817048 None:intergenic 45.0%
!! ATTTCTAGCGAACGATGCGT+CGG - chr4.4:80817327-80817346 MS.gene048143:intron 45.0%
!! GAAGTTGATGAAGATGGTGG+TGG + chr4.4:80817036-80817055 None:intergenic 45.0%
!! GGTGGTGGTGTTGATGAAAA+CGG - chr4.4:80817483-80817502 MS.gene048143:intron 45.0%
!! GTGGAAGTTGATGAAGATGG+TGG + chr4.4:80817039-80817058 None:intergenic 45.0%
!! GTGGTGGTGGAAATTGGTTA+TGG + chr4.4:80817010-80817029 None:intergenic 45.0%
!! GTTAGTTATGGTGGTGGAAG+TGG + chr4.4:80816985-80817004 None:intergenic 45.0%
!! TAGTCGTTTTAAGCCTCCAG+GGG - chr4.4:80816517-80816536 MS.gene048143:CDS 45.0%
!! TGGTTTGTGGTGGTGGAAAT+TGG + chr4.4:80817016-80817035 None:intergenic 45.0%
AATGACCAAAATGCCCCTGG+AGG + chr4.4:80816533-80816552 None:intergenic 50.0%
GGAGGCTGTTACAATGGTTG+TGG + chr4.4:80817058-80817077 None:intergenic 50.0%
GTGGAAGTGGTGGTTTATGG+TGG + chr4.4:80816972-80816991 None:intergenic 50.0%
GTGGTTATGGAGGTTTGTGG+TGG + chr4.4:80816953-80816972 None:intergenic 50.0%
TCGAATCTGAGTTGCACGCA+AGG - chr4.4:80817270-80817289 MS.gene048143:intron 50.0%
! ACACCTTCCTTTTACACCGG+CGG - chr4.4:80817671-80817690 MS.gene048143:intron 50.0%
! GAGGTTGAAGTTTGGGGCTT+TGG - chr4.4:80816032-80816051 MS.gene048143:CDS 50.0%
! GTGGTGGAAGTGGTGGTTTA+TGG + chr4.4:80816975-80816994 None:intergenic 50.0%
! TGAGTCAGTTTTGCCCCTAG+GGG - chr4.4:80816358-80816377 MS.gene048143:CDS 50.0%
!! AAGATGGTGGTGGTTTGTGG+TGG + chr4.4:80817026-80817045 None:intergenic 50.0%
!! AGTTATGGTGGTGGAAGTGG+TGG + chr4.4:80816982-80817001 None:intergenic 50.0%
!!! TTAAGCCTCCAGGGGCATTT+TGG - chr4.4:80816525-80816544 MS.gene048143:CDS 50.0%
!!! TTTTGCCCCTAGGGGCATTT+TGG - chr4.4:80816366-80816385 MS.gene048143:CDS 50.0%
AGGACTTCCCAGAACGATCG+GGG + chr4.4:80817611-80817630 None:intergenic 55.0%
CAGGACTTCCCAGAACGATC+GGG + chr4.4:80817612-80817631 None:intergenic 55.0%
GCAGGACTTCCCAGAACGAT+CGG + chr4.4:80817613-80817632 None:intergenic 55.0%
GCTGATTACACCACCGCAGA+CGG + chr4.4:80817635-80817654 None:intergenic 55.0%
!! ATGGTGGTGGTTTGTGGTGG+TGG + chr4.4:80817023-80817042 None:intergenic 55.0%
AACGGAGTCCCCGATCGTTC+TGG - chr4.4:80817600-80817619 MS.gene048143:intron 60.0%
ACGGAGTCCCCGATCGTTCT+GGG - chr4.4:80817601-80817620 MS.gene048143:intron 60.0%
ATTACACCACCGCAGACGGC+AGG + chr4.4:80817631-80817650 None:intergenic 60.0%
CCACCGCCGGTGTAAAAGGA+AGG + chr4.4:80817677-80817696 None:intergenic 60.0%
! CCTTCCTTTTACACCGGCGG+TGG - chr4.4:80817674-80817693 MS.gene048143:intron 60.0%
! TCCTTTTACACCGGCGGTGG+TGG - chr4.4:80817677-80817696 MS.gene048143:intron 60.0%
CTGGGAAGTCCTGCCGTCTG+CGG - chr4.4:80817619-80817638 MS.gene048143:intron 65.0%
GATCGTTGTGCCACCACCGC+CGG + chr4.4:80817690-80817709 None:intergenic 65.0%
GCCACCACCGCCGGTGTAAA+AGG + chr4.4:80817681-80817700 None:intergenic 65.0%
GGAAGTCCTGCCGTCTGCGG+TGG - chr4.4:80817622-80817641 MS.gene048143:intron 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 80816012 80817758 80816012 ID=MS.gene048143
chr4.4 mRNA 80816012 80817758 80816012 ID=MS.gene048143.t1;Parent=MS.gene048143
chr4.4 exon 80817736 80817758 80817736 ID=MS.gene048143.t1.exon1;Parent=MS.gene048143.t1
chr4.4 CDS 80817736 80817758 80817736 ID=cds.MS.gene048143.t1;Parent=MS.gene048143.t1
chr4.4 exon 80816012 80816570 80816012 ID=MS.gene048143.t1.exon2;Parent=MS.gene048143.t1
chr4.4 CDS 80816012 80816570 80816012 ID=cds.MS.gene048143.t1;Parent=MS.gene048143.t1
Gene Sequence

>MS.gene048143

ATGTCTTTCAAATTCTTGTTGAGGTTGAAGTTTGGGGCTTTGGTAAGATTAATCATTTGAATATGGTTGGTGTTATACCCTAATTTTGGACCTAAAAATACTAACTCAACTCTTCTTTTCTTGCATTTCAAGTCATTTGATATGTCTCTGATCAATTGCATCTTTCTCTGCATTTTATTTCAGTATTGTTTGCAAAATTCATGAAAAAGGGCATTTTAGTCATTTCCTGTGTCAAAATTTCTTTCAGTCTTTTAAAAATTTGTTTGAGTCCTTTCAAGTAGTTTGAATAAGTCTCTGTTTCATTCTATGTCAAAGTCCAATTCAAAATTTGCATTTGAAGTCATTTTGAGTCAGTTTTGCCCCTAGGGGCATTTTGGTCTTTTCATGTGTCAAAACTCTGTTTAAATTCCCAAAATTGTCTCTGAATCTTTTAATTTTGTTTGTATAAGTCTTTGCTTCAATTCCTTGTTAAAAATCCATTTTAAAAATTCATCTGAGTCATTTTTAGTCGTTTTAAGCCTCCAGGGGCATTTTGGTCATTTCACATAAAATTGCTGATTCATTTTGATCTGCTTTGTTGCGTTTAATCGTCTCGCTTTACGTTAATTTTCAATTTATTACTTTCAGTTCAATTTTAGTTTTAATTTTCATTTTGGTCCTCGTACTTTTGTTATTTGCATTTTAATCCTTTTTAAAGTTTGTTAGAGTCAAAGTCAAGGTTTGTTACAAGTCAAGTGCCACATGGCATTGTTTCATTGGTCCAAGCATTCCCACATCACCTATAAATAGGTCAAAGTTGGTCAAGGTTCATGGAACTAATCATTTTTTTTACACCAATTTTTATTTTTCAATCATTTTTTACAAAATCACAATATTCATAATTATTTTTCCCCCACTTTCTCTCTCCTCTAACTAACTTCATCTTCAACCTCTAACAACCACCACAAACCTCCATAACCACCATAAACCACCACTTCCACCACCATAACTAACCTCCATAACCAATTTCCACCACCACAAACCACCACCATCTTCATCAACTTCCACAACCATTGTAACAGCCTCCAATTCCTAACCAAATCCAACCCAATAATTCACAATCGATTTTGTTTTCCTTCATTTCATCAATTCTTTTTTCCGTTCTTCGGTTCAAACATTCCAATCTCTCAACATCACTATTGCTCGAAGTCCAAACGAAACCAACAACAAACGATTCCGATACGCTTCTGCGATCGATTTCAGCAACGTTTGTTTGGGCTCGAATCTGAGTTGCACGCAAGGTCTCAAATACAATTCAGAACCACGCAGAACTGAGATTTCTAGCGAACGATGCGTCGGATTCGCGAATTTGAAGTTGAAGAACAGAAACAAACACGTTGGAAGTAGTTACATACACATACACAACAAAAACAGTTTTAAGGTAGAAGAAAAGAGGATCAGTGCAAATTCGAGTTCCAATTCGAATTCAAACGGTGGTGGTGTTGATGAAAACGGTGATAAAGTGAAGAGTTCATGGAGTAAAAACGAAGATGAAACATTGGTGAAACTGGTGAAGGAAAACGGTGCAAGAAACTGGTCTGTGATAAGCAACGGAGTCCCCGATCGTTCTGGGAAGTCCTGCCGTCTGCGGTGGTGTAATCAGCTGAGTCCAACCGTTCAACACCTTCCTTTTACACCGGCGGTGGTGGCACAACGATCATCGACGAGATTTACTAATTCTGTGCACCAAGATCCTTCGATTTCTTGA
Protein sequence

>MS.gene048143.t1

MSFKFLLSPNETNNKRFRYASAIDFSNVCLGSNLSCTQGLKYNSEPRRTEISSERCVGFANLKLKNRNKHVGSSYIHIHNKNSFKVEEKRISANSSSNSNSNGGGVDENGDKVKSSWSKNEDETLVKLVKENGARNWSVISNGVPDRSGKSCRLRWCNQLSPTVQHLPFTPAVVAQRSSTRFTNSVHQDPSIS