Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048151.t1 | XP_024632961.1 | 86.8 | 311 | 17 | 3 | 1 | 287 | 1 | 311 | 2.00E-130 | 475.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048151.t1 | Q9SZL8 | 27.1 | 255 | 173 | 5 | 42 | 290 | 72 | 319 | 9.4e-20 | 99.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048151.t1 | A0A072TE13 | 80.9 | 303 | 35 | 3 | 1 | 287 | 1 | 296 | 6.0e-129 | 469.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene048151.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048151.t1 | MTR_0558s0020 | 86.799 | 303 | 17 | 3 | 1 | 287 | 1 | 296 | 0.0 | 531 |
| MS.gene048151.t1 | MTR_1g083640 | 85.315 | 286 | 18 | 3 | 1 | 262 | 1 | 286 | 9.18e-177 | 489 |
| MS.gene048151.t1 | MTR_8g446020 | 73.927 | 303 | 16 | 4 | 1 | 287 | 1 | 256 | 1.21e-151 | 424 |
| MS.gene048151.t1 | MTR_2g014100 | 85.714 | 245 | 18 | 3 | 1 | 228 | 1 | 245 | 6.77e-147 | 412 |
| MS.gene048151.t1 | MTR_6g090505 | 86.522 | 230 | 15 | 2 | 73 | 286 | 1 | 230 | 5.89e-142 | 404 |
| MS.gene048151.t1 | MTR_0337s0040 | 69.403 | 268 | 20 | 4 | 1 | 252 | 1 | 222 | 4.39e-120 | 343 |
| MS.gene048151.t1 | MTR_3g008240 | 80.476 | 210 | 18 | 3 | 1 | 194 | 1 | 203 | 1.08e-114 | 329 |
| MS.gene048151.t1 | MTR_4g040430 | 79.524 | 210 | 15 | 4 | 1 | 194 | 1 | 198 | 3.28e-111 | 320 |
| MS.gene048151.t1 | MTR_5g071590 | 73.333 | 180 | 27 | 3 | 1 | 159 | 1 | 180 | 1.91e-88 | 262 |
| MS.gene048151.t1 | MTR_0019s0290 | 78.947 | 152 | 16 | 2 | 90 | 225 | 9 | 160 | 1.01e-78 | 236 |
| MS.gene048151.t1 | MTR_1g055240 | 82.517 | 143 | 9 | 2 | 98 | 224 | 2 | 144 | 3.16e-78 | 234 |
| MS.gene048151.t1 | MTR_1281s0010 | 85.294 | 102 | 7 | 1 | 27 | 120 | 1 | 102 | 1.03e-55 | 176 |
| MS.gene048151.t1 | MTR_8g046060 | 88.636 | 88 | 9 | 1 | 1 | 87 | 91 | 178 | 1.01e-42 | 146 |
| MS.gene048151.t1 | MTR_4g008820 | 82.759 | 87 | 8 | 1 | 1 | 87 | 125 | 204 | 1.64e-38 | 143 |
| MS.gene048151.t1 | MTR_6g051425 | 30.417 | 240 | 154 | 4 | 48 | 280 | 22 | 255 | 9.57e-25 | 102 |
| MS.gene048151.t1 | MTR_1g070355 | 27.984 | 243 | 156 | 7 | 49 | 283 | 80 | 311 | 1.68e-16 | 79.7 |
| MS.gene048151.t1 | MTR_0337s0050 | 83.333 | 36 | 6 | 0 | 251 | 286 | 14 | 49 | 1.07e-14 | 68.2 |
| MS.gene048151.t1 | MTR_1g013060 | 28.241 | 216 | 120 | 9 | 48 | 245 | 29 | 227 | 4.90e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048151.t1 | AT4G38180 | 27.059 | 255 | 173 | 5 | 42 | 290 | 72 | 319 | 8.22e-21 | 92.8 |
| MS.gene048151.t1 | AT5G28530 | 25.101 | 247 | 165 | 5 | 48 | 279 | 60 | 301 | 1.87e-14 | 73.9 |
| MS.gene048151.t1 | AT5G28530 | 25.101 | 247 | 165 | 5 | 48 | 279 | 60 | 301 | 1.87e-14 | 73.9 |
| MS.gene048151.t1 | AT5G28530 | 25.101 | 247 | 165 | 5 | 48 | 279 | 60 | 301 | 1.91e-14 | 73.9 |
| MS.gene048151.t1 | AT1G10240 | 23.504 | 234 | 175 | 4 | 57 | 287 | 62 | 294 | 1.45e-11 | 65.1 |
| MS.gene048151.t1 | AT1G10240 | 23.504 | 234 | 175 | 4 | 57 | 287 | 62 | 294 | 1.45e-11 | 65.1 |
Find 37 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACTAGATTATTCTCTATT+TGG | 0.147857 | 6.2:+29312066 | MS.gene048151:CDS |
| ATAACACTGGATTCTCTATT+AGG | 0.309117 | 6.2:+29311476 | MS.gene048151:CDS |
| ACAAACTCAATAAAGTTGTT+AGG | 0.316138 | 6.2:-29311607 | None:intergenic |
| GGCGATATTGATTGTTTGTT+CGG | 0.357702 | 6.2:-29312116 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 6.2:+29311825 | MS.gene048151:CDS |
| GTACAAAATACTAACCTAAT+GGG | 0.422907 | 6.2:-29312137 | None:intergenic |
| ATGTCTATAAGATATATAGA+TGG | 0.423693 | 6.2:+29311680 | MS.gene048151:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 6.2:+29311838 | MS.gene048151:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 6.2:+29311763 | MS.gene048151:CDS |
| AACAATCAATATCGCCCATT+AGG | 0.430510 | 6.2:+29312123 | MS.gene048151:CDS |
| TCCATGATTCTTTATTGCTA+GGG | 0.444266 | 6.2:-29311396 | None:intergenic |
| TTCCATGATTCTTTATTGCT+AGG | 0.445442 | 6.2:-29311397 | None:intergenic |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 6.2:+29311906 | MS.gene048151:CDS |
| GCTTTAATAAATTCTAAAGC+TGG | 0.465202 | 6.2:+29311793 | MS.gene048151:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 6.2:+29311826 | MS.gene048151:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 6.2:+29311559 | MS.gene048151:CDS |
| CCCCTAGCAATAAAGAATCA+TGG | 0.497001 | 6.2:+29311395 | MS.gene048151:CDS |
| TGTCTTGTGCAGCATCAACT+TGG | 0.502977 | 6.2:-29312011 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 6.2:+29311570 | MS.gene048151:CDS |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 6.2:+29311463 | MS.gene048151:CDS |
| TGTCTATAAGATATATAGAT+GGG | 0.523615 | 6.2:+29311681 | MS.gene048151:CDS |
| TTTAAGTCCGAAAACCGAAA+AGG | 0.524077 | 6.2:-29311877 | None:intergenic |
| AAATGCCCTGGTTTATGTAA+TGG | 0.529222 | 6.2:-29311721 | None:intergenic |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 6.2:+29311525 | MS.gene048151:CDS |
| TAGACACTAAGCAGTTCAAA+AGG | 0.536783 | 6.2:-29312237 | None:intergenic |
| CCATGATTCTTTATTGCTAG+GGG | 0.537011 | 6.2:-29311395 | None:intergenic |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 6.2:+29311571 | MS.gene048151:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 6.2:+29311919 | MS.gene048151:CDS |
| CTCAAGTAACACAAATGCCC+TGG | 0.558368 | 6.2:-29311733 | None:intergenic |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 6.2:+29311912 | MS.gene048151:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.567659 | 6.2:+29311687 | MS.gene048151:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 6.2:+29311841 | MS.gene048151:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 6.2:+29311832 | MS.gene048151:CDS |
| TGTCATCCATTACATAAACC+AGG | 0.618164 | 6.2:+29311715 | MS.gene048151:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 6.2:+29311915 | MS.gene048151:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 6.2:+29311846 | MS.gene048151:CDS |
| GTCATCCATTACATAAACCA+GGG | 0.682651 | 6.2:+29311716 | MS.gene048151:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | + | chr6.2:29311525-29311544 | MS.gene048151:CDS | 20.0% |
| !! | ATGTCTATAAGATATATAGA+TGG | + | chr6.2:29311680-29311699 | MS.gene048151:CDS | 20.0% |
| !! | TGTCTATAAGATATATAGAT+GGG | + | chr6.2:29311681-29311700 | MS.gene048151:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | + | chr6.2:29311442-29311461 | MS.gene048151:CDS | 20.0% |
| ! | ACAAACTCAATAAAGTTGTT+AGG | - | chr6.2:29311610-29311629 | None:intergenic | 25.0% |
| ! | AGTACAAAATACTAACCTAA+TGG | - | chr6.2:29312141-29312160 | None:intergenic | 25.0% |
| ! | CAACTAGATTATTCTCTATT+TGG | + | chr6.2:29312066-29312085 | MS.gene048151:CDS | 25.0% |
| ! | GTACAAAATACTAACCTAAT+GGG | - | chr6.2:29312140-29312159 | None:intergenic | 25.0% |
| ! | TGGATATCATAGTAAAAAGA+TGG | - | chr6.2:29311994-29312013 | None:intergenic | 25.0% |
| !! | CATTATGTTGTATTTTGTTC+TGG | + | chr6.2:29312191-29312210 | MS.gene048151:intron | 25.0% |
| !!! | ACTTGGAATAATTTTGAAGT+TGG | + | chr6.2:29311645-29311664 | MS.gene048151:CDS | 25.0% |
| !!! | GCTTTAATAAATTCTAAAGC+TGG | + | chr6.2:29311793-29311812 | MS.gene048151:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | + | chr6.2:29311571-29311590 | MS.gene048151:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | + | chr6.2:29311826-29311845 | MS.gene048151:CDS | 30.0% | |
| ATAACACTGGATTCTCTATT+AGG | + | chr6.2:29311476-29311495 | MS.gene048151:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | + | chr6.2:29311559-29311578 | MS.gene048151:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | + | chr6.2:29311463-29311482 | MS.gene048151:CDS | 30.0% | |
| GTGAAAGTGAAAGAAAAACT+TGG | + | chr6.2:29311628-29311647 | MS.gene048151:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | + | chr6.2:29311570-29311589 | MS.gene048151:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | + | chr6.2:29311687-29311706 | MS.gene048151:CDS | 30.0% | |
| TCCATGATTCTTTATTGCTA+GGG | - | chr6.2:29311399-29311418 | None:intergenic | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | + | chr6.2:29311906-29311925 | MS.gene048151:CDS | 30.0% | |
| TTCCATGATTCTTTATTGCT+AGG | - | chr6.2:29311400-29311419 | None:intergenic | 30.0% | |
| ! | TAGTATTTTGTACTTCTCTC+AGG | + | chr6.2:29312147-29312166 | MS.gene048151:intron | 30.0% |
| !!! | AAGACATAGCTAGCATTTTT+TGG | + | chr6.2:29312028-29312047 | MS.gene048151:CDS | 30.0% |
| !!! | AGACATAGCTAGCATTTTTT+GGG | + | chr6.2:29312029-29312048 | MS.gene048151:CDS | 30.0% |
| AAATGCCCTGGTTTATGTAA+TGG | - | chr6.2:29311724-29311743 | None:intergenic | 35.0% | |
| AACAATCAATATCGCCCATT+AGG | + | chr6.2:29312123-29312142 | MS.gene048151:CDS | 35.0% | |
| CCATGATTCTTTATTGCTAG+GGG | - | chr6.2:29311398-29311417 | None:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | + | chr6.2:29311763-29311782 | MS.gene048151:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | + | chr6.2:29311825-29311844 | MS.gene048151:CDS | 35.0% | |
| GTCATCCATTACATAAACCA+GGG | + | chr6.2:29311716-29311735 | MS.gene048151:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | + | chr6.2:29311832-29311851 | MS.gene048151:CDS | 35.0% | |
| TGTCATCCATTACATAAACC+AGG | + | chr6.2:29311715-29311734 | MS.gene048151:CDS | 35.0% | |
| TTTAAGTCCGAAAACCGAAA+AGG | - | chr6.2:29311880-29311899 | None:intergenic | 35.0% | |
| ! | GAATCTTCCTTTTCGGTTTT+CGG | + | chr6.2:29311870-29311889 | MS.gene048151:CDS | 35.0% |
| ! | GGCGATATTGATTGTTTGTT+CGG | - | chr6.2:29312119-29312138 | None:intergenic | 35.0% |
| ! | TGTTGTATTTTGTTCTGGCA+TGG | + | chr6.2:29312196-29312215 | MS.gene048151:intron | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | + | chr6.2:29311915-29311934 | MS.gene048151:CDS | 40.0% | |
| CCCCTAGCAATAAAGAATCA+TGG | + | chr6.2:29311395-29311414 | MS.gene048151:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | + | chr6.2:29311912-29311931 | MS.gene048151:CDS | 40.0% | |
| ! | TGTTCTGGCATGGAAAGTAT+AGG | + | chr6.2:29312206-29312225 | MS.gene048151:intron | 40.0% |
| !!! | GCTAGCATTTTTTGGGCATA+TGG | + | chr6.2:29312036-29312055 | MS.gene048151:CDS | 40.0% |
| CTCAAGTAACACAAATGCCC+TGG | - | chr6.2:29311736-29311755 | None:intergenic | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | + | chr6.2:29311838-29311857 | MS.gene048151:CDS | 45.0% | |
| TGTCTTGTGCAGCATCAACT+TGG | - | chr6.2:29312014-29312033 | None:intergenic | 45.0% | |
| ! | CTGGCATGGAAAGTATAGGT+AGG | + | chr6.2:29312210-29312229 | MS.gene048151:intron | 45.0% |
| ! | GCACGGAGAATCTTCCTTTT+CGG | + | chr6.2:29311863-29311882 | MS.gene048151:CDS | 45.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | + | chr6.2:29311841-29311860 | MS.gene048151:CDS | 50.0% | |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | + | chr6.2:29311919-29311938 | MS.gene048151:CDS | 50.0% |
| GGGTCAAGGAGTTGGAGGCA+CGG | + | chr6.2:29311846-29311865 | MS.gene048151:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 29311286 | 29312244 | 29311286 | ID=MS.gene048151 |
| chr6.2 | mRNA | 29311286 | 29312244 | 29311286 | ID=MS.gene048151.t1;Parent=MS.gene048151 |
| chr6.2 | exon | 29311286 | 29312144 | 29311286 | ID=MS.gene048151.t1.exon1;Parent=MS.gene048151.t1 |
| chr6.2 | CDS | 29311286 | 29312144 | 29311286 | ID=cds.MS.gene048151.t1;Parent=MS.gene048151.t1 |
| chr6.2 | exon | 29312228 | 29312244 | 29312228 | ID=MS.gene048151.t1.exon2;Parent=MS.gene048151.t1 |
| chr6.2 | CDS | 29312228 | 29312244 | 29312228 | ID=cds.MS.gene048151.t1;Parent=MS.gene048151.t1 |
| Gene Sequence |
| Protein sequence |
