AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048211

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048211.t1 MTR_2g026250 90.516 601 50 3 1 597 1 598 0.0 1115
MS.gene048211.t1 MTR_4g133660 54.711 605 204 9 1 596 1 544 0.0 618
MS.gene048211.t1 MTR_5g097480 47.107 605 249 12 1 595 1 544 0.0 525
MS.gene048211.t1 MTR_3g056110 54.588 425 189 3 174 595 119 542 4.97e-171 497
MS.gene048211.t1 MTR_5g094450 56.959 388 162 3 212 595 146 532 8.03e-160 468
MS.gene048211.t1 MTR_2g082090 54.450 382 167 2 216 594 201 578 1.64e-147 438
MS.gene048211.t1 MTR_1g029420 47.709 371 193 1 225 595 223 592 8.82e-128 388
MS.gene048211.t1 MTR_3g089055 47.709 371 193 1 225 595 196 565 1.24e-123 376
MS.gene048211.t1 MTR_3g089055 47.709 371 193 1 225 595 201 570 1.40e-123 376
MS.gene048211.t1 MTR_7g027190 35.162 401 213 8 222 596 295 674 9.92e-71 241
MS.gene048211.t1 MTR_4g064160 32.275 378 246 3 222 594 311 683 5.16e-64 223
MS.gene048211.t1 MTR_4g064150 30.423 378 257 4 222 594 356 732 4.37e-62 218
MS.gene048211.t1 MTR_4g064200 32.275 378 244 6 222 594 279 649 2.72e-61 215
MS.gene048211.t1 MTR_7g062120 29.867 375 261 2 222 595 367 740 6.92e-61 215
MS.gene048211.t1 MTR_2g097390 30.851 376 252 4 222 594 270 640 1.31e-59 210
MS.gene048211.t1 MTR_2g097310 32.095 377 246 6 222 594 267 637 1.47e-59 210
MS.gene048211.t1 MTR_6g047750 33.079 393 218 9 225 595 293 662 6.31e-58 206
MS.gene048211.t1 MTR_7g057230 34.706 340 210 5 261 594 323 656 1.49e-57 204
MS.gene048211.t1 MTR_2g097463 29.679 374 258 3 222 594 285 654 2.49e-56 201
MS.gene048211.t1 MTR_2g097350 31.481 378 247 6 222 594 269 639 2.78e-56 201
MS.gene048211.t1 MTR_2g097467 29.443 377 261 4 219 594 281 653 3.09e-56 201
MS.gene048211.t1 MTR_2g097473 30.667 375 253 6 222 594 284 653 6.62e-56 200
MS.gene048211.t1 MTR_4g064120 28.947 380 256 5 222 592 249 623 9.78e-56 199
MS.gene048211.t1 MTR_2g097410 29.630 378 258 5 222 594 366 740 1.74e-55 200
MS.gene048211.t1 MTR_4g064180 28.684 380 257 5 222 592 249 623 3.36e-54 195
MS.gene048211.t1 MTR_3g065980 31.043 393 229 11 224 595 169 540 8.66e-54 192
MS.gene048211.t1 MTR_4g104020 32.391 389 238 8 222 596 144 521 9.83e-54 191
MS.gene048211.t1 MTR_7g074650 32.425 367 232 7 230 595 441 792 2.10e-51 189
MS.gene048211.t1 MTR_8g442410 30.026 383 252 6 222 595 150 525 6.00e-49 179
MS.gene048211.t1 MTR_4g097080 29.096 354 238 5 250 595 155 503 1.07e-48 177
MS.gene048211.t1 MTR_3g022830 27.231 437 236 10 218 595 23 436 1.50e-47 172
MS.gene048211.t1 MTR_4g102790 31.461 356 223 9 251 594 315 661 8.35e-45 169
MS.gene048211.t1 MTR_2g097380 28.530 347 216 4 249 594 245 560 1.30e-44 167
MS.gene048211.t1 MTR_5g015490 29.558 362 232 8 250 595 136 490 3.19e-44 164
MS.gene048211.t1 MTR_4g077760 31.728 353 220 10 251 595 216 555 1.14e-41 159
MS.gene048211.t1 MTR_1g096030 30.688 378 208 14 251 595 103 459 3.11e-40 153
MS.gene048211.t1 MTR_2g034250 26.615 387 265 7 222 595 205 585 2.85e-38 149
MS.gene048211.t1 MTR_4g076020 29.363 361 218 11 244 595 97 429 3.00e-37 144
MS.gene048211.t1 MTR_2g034280 25.974 385 267 9 222 595 198 575 2.74e-36 143
MS.gene048211.t1 MTR_3g072710 27.778 360 225 10 259 596 155 501 2.87e-36 142
MS.gene048211.t1 MTR_2g034260 26.234 385 264 9 224 595 207 584 3.16e-36 143
MS.gene048211.t1 MTR_1g069725 28.994 338 218 9 263 596 145 464 1.88e-34 136
MS.gene048211.t1 MTR_5g058860 27.654 358 233 12 256 596 150 498 6.14e-33 132
MS.gene048211.t1 MTR_7g109580 27.089 347 237 8 256 595 217 554 8.13e-33 133
MS.gene048211.t1 MTR_2g089100 26.551 403 255 11 224 594 49 442 8.15e-33 131
MS.gene048211.t1 MTR_2g099110 27.049 366 237 8 250 595 96 451 6.07e-32 129
MS.gene048211.t1 MTR_4g026485 25.858 379 252 11 226 596 268 625 2.27e-28 120
MS.gene048211.t1 MTR_5g015950 26.168 321 201 6 308 594 127 445 2.36e-28 118
MS.gene048211.t1 MTR_3g053270 26.168 321 201 6 308 594 127 445 2.36e-28 118
MS.gene048211.t1 MTR_1g086970 25.429 350 238 8 259 595 139 478 3.29e-28 118
MS.gene048211.t1 MTR_4g095500 24.940 417 262 9 224 594 56 467 4.11e-28 118
MS.gene048211.t1 MTR_8g093070 27.887 355 214 16 255 594 168 495 6.98e-26 112
MS.gene048211.t1 MTR_5g009080 31.050 219 138 5 228 441 57 267 2.71e-25 109
MS.gene048211.t1 MTR_5g009080 35.915 142 90 1 456 596 338 479 8.26e-23 102
MS.gene048211.t1 MTR_4g076140 39.007 141 85 1 456 595 329 469 4.71e-24 105
MS.gene048211.t1 MTR_4g076140 29.952 207 137 3 228 433 49 248 5.52e-23 102
MS.gene048211.t1 MTR_0092s0100 24.802 379 257 10 226 596 371 729 7.19e-24 107
MS.gene048211.t1 MTR_0092s0100 24.802 379 257 10 226 596 374 732 8.48e-24 106
MS.gene048211.t1 MTR_8g020840 24.938 401 249 15 226 595 169 548 1.03e-22 102
MS.gene048211.t1 MTR_3g022580 30.000 110 77 0 486 595 74 183 7.96e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048211.t1 AT4G17230 46.922 601 237 10 1 595 1 525 2.67e-178 515
MS.gene048211.t1 AT4G17230 46.922 601 237 10 1 595 1 525 2.67e-178 515
MS.gene048211.t1 AT5G48150 55.300 434 181 4 168 595 64 490 4.19e-167 485
MS.gene048211.t1 AT5G48150 55.300 434 181 4 168 595 64 490 4.19e-167 485
MS.gene048211.t1 AT5G48150 55.300 434 181 4 168 595 64 490 4.19e-167 485
MS.gene048211.t1 AT5G48150 55.300 434 181 4 168 595 64 490 4.19e-167 485
MS.gene048211.t1 AT1G50600 53.919 421 191 2 175 595 109 526 1.09e-160 470
MS.gene048211.t1 AT1G50600 53.919 421 191 2 175 595 180 597 5.75e-160 471
MS.gene048211.t1 AT1G50600 53.919 421 191 2 175 595 184 601 7.12e-160 471
MS.gene048211.t1 AT2G04890 54.964 413 177 3 184 595 9 413 7.46e-159 461
MS.gene048211.t1 AT1G21450 45.526 380 205 2 217 595 215 593 1.30e-119 367
MS.gene048211.t1 AT1G07530 31.481 378 248 4 223 594 392 764 5.74e-62 219
MS.gene048211.t1 AT3G03450 32.979 376 238 9 224 595 180 545 3.03e-61 213
MS.gene048211.t1 AT5G52510 34.195 348 204 7 263 595 303 640 1.87e-59 209
MS.gene048211.t1 AT5G59450 30.423 378 258 4 222 595 222 598 2.26e-59 209
MS.gene048211.t1 AT5G17490 30.505 495 302 16 125 595 40 516 6.47e-59 206
MS.gene048211.t1 AT3G46600 30.563 373 256 3 224 595 1 371 5.04e-58 199
MS.gene048211.t1 AT3G46600 30.667 375 257 3 222 595 175 547 7.20e-58 203
MS.gene048211.t1 AT3G46600 30.667 375 257 3 222 595 207 579 8.79e-58 204
MS.gene048211.t1 AT2G37650 30.607 379 251 4 222 595 342 713 1.20e-57 206
MS.gene048211.t1 AT2G37650 30.607 379 251 4 222 595 330 701 1.46e-57 206
MS.gene048211.t1 AT1G14920 31.332 383 230 10 224 595 169 529 1.79e-57 202
MS.gene048211.t1 AT2G29060 30.526 380 251 6 223 594 280 654 2.18e-57 204
MS.gene048211.t1 AT2G29060 30.526 380 251 6 223 594 317 691 3.40e-57 204
MS.gene048211.t1 AT2G01570 31.152 382 232 11 224 595 221 581 9.19e-55 196
MS.gene048211.t1 AT1G66350 31.316 380 230 12 224 596 152 507 2.21e-54 193
MS.gene048211.t1 AT1G50420 28.837 430 238 8 228 596 58 480 2.95e-54 192
MS.gene048211.t1 AT2G29065 30.105 382 257 7 222 594 247 627 3.67e-53 192
MS.gene048211.t1 AT1G07520 29.396 381 260 6 222 594 149 528 1.89e-51 185
MS.gene048211.t1 AT1G07520 29.396 381 260 6 222 594 149 528 1.89e-51 185
MS.gene048211.t1 AT1G07520 29.396 381 260 6 222 594 313 692 7.33e-51 187
MS.gene048211.t1 AT1G07520 29.396 381 260 6 222 594 314 693 7.56e-51 187
MS.gene048211.t1 AT3G54220 31.165 369 235 8 230 594 296 649 1.01e-46 174
MS.gene048211.t1 AT5G66770 33.811 349 213 7 255 595 246 584 1.57e-45 170
MS.gene048211.t1 AT3G50650 32.286 350 219 7 255 595 202 542 1.89e-44 166
MS.gene048211.t1 AT4G37650 30.245 367 230 9 250 595 168 529 1.39e-41 158
MS.gene048211.t1 AT1G63100 29.944 354 223 6 258 594 308 653 3.10e-41 159
MS.gene048211.t1 AT1G63100 29.944 354 223 6 258 594 283 628 3.33e-41 158
MS.gene048211.t1 AT5G41920 28.000 375 245 9 228 595 44 400 1.37e-40 152
MS.gene048211.t1 AT1G55580 26.129 310 197 7 305 595 149 445 1.04e-30 125
MS.gene048211.t1 AT4G08250 25.641 351 248 7 251 594 132 476 3.66e-29 121
MS.gene048211.t1 AT3G49950 23.967 363 251 10 250 594 52 407 5.89e-25 108
MS.gene048211.t1 AT3G60630 24.716 352 230 10 261 596 291 623 1.30e-22 102
MS.gene048211.t1 AT2G45160 25.978 358 215 13 260 596 312 640 2.19e-21 99.0
MS.gene048211.t1 AT4G00150 24.138 377 256 11 224 596 205 555 9.73e-20 93.6
MS.gene048211.t1 AT3G13840 23.464 358 245 10 250 595 170 510 2.18e-19 92.0
MS.gene048211.t1 AT4G36710 23.969 388 259 12 222 595 120 485 9.78e-18 87.0

Find 110 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAAGCAAATTGAAAATA+TGG 0.174043 2.3:-63064178 None:intergenic
AAGATTTCAATAAAGATTAT+AGG 0.216624 2.3:+63064941 MS.gene048211:CDS
TCAAAGAACCAATGTTTCTT+TGG 0.242227 2.3:+63063385 MS.gene048211:CDS
TGAGAAAATTGTGGTGAAAA+AGG 0.281191 2.3:-63063524 None:intergenic
AAAGCTCAATTTCTTTCATT+TGG 0.293718 2.3:-63063631 None:intergenic
CCTCCGTTCGTTCGTGTAAC+AGG 0.298231 2.3:+63064328 MS.gene048211:CDS
ACTGTGATCCTTGTGCAATT+TGG 0.334053 2.3:-63064264 None:intergenic
GATTTCAGAGCTAGGCTATA+AGG 0.338912 2.3:+63063763 MS.gene048211:CDS
CTCATCAACTCATTGCTTGT+TGG 0.342988 2.3:-63064127 None:intergenic
TTGCAAATCAATTCAAATAA+TGG 0.345919 2.3:+63063353 MS.gene048211:CDS
GCTGTCGATGATATCTGAAT+TGG 0.360875 2.3:-63063810 None:intergenic
TTAACACTATTGAACTCAAA+TGG 0.361949 2.3:-63064442 None:intergenic
TAAGCACCTAACCTTTGAAT+TGG 0.365266 2.3:-63064031 None:intergenic
TCTTCTTTCAAGTCTAGCTT+TGG 0.368681 2.3:-63063908 None:intergenic
ATTGAAAGACATGAACTGTT+TGG 0.379235 2.3:+63064832 MS.gene048211:CDS
AATTTGAGACCAATCATATT+CGG 0.388708 2.3:-63063873 None:intergenic
TTTCTTTGGAAACAAGCAAC+TGG 0.406710 2.3:+63063399 MS.gene048211:CDS
GAAAGAAATTGAGCTTTCAT+TGG 0.409492 2.3:+63063637 MS.gene048211:CDS
TTCAATAGTGTTAAAATGTA+TGG 0.409922 2.3:+63064451 MS.gene048211:CDS
TCTGTCTCAAAGACTGTTGC+TGG 0.413091 2.3:-63063301 None:intergenic
GAATCGGAGATTTCATTGTT+AGG 0.417405 2.3:+63063785 MS.gene048211:CDS
GTATGGATGTGAGGTTCAAC+TGG 0.437985 2.3:+63064468 MS.gene048211:CDS
ATCAAAAGCTATGAATTCAT+TGG 0.443080 2.3:-63063459 None:intergenic
AGCTTCCAGTCCAACTTAAA+CGG 0.448434 2.3:+63063830 MS.gene048211:CDS
GCCTAAGGTCGTGTTATTTG+TGG 0.450027 2.3:+63064621 MS.gene048211:CDS
AGAGCAAGGGTGGAATCATC+GGG 0.455587 2.3:+63064070 MS.gene048211:CDS
GGTTCTCCAATTCAAAGGTT+AGG 0.459338 2.3:+63064025 MS.gene048211:CDS
TGTAAGCAAATTGAAAATAT+GGG 0.460197 2.3:-63064177 None:intergenic
GAAATTGAGCTTTCATTGGT+AGG 0.460607 2.3:+63063641 MS.gene048211:CDS
GGTGGAAGACTTGATATTGT+AGG 0.469286 2.3:+63064382 MS.gene048211:CDS
GGTCGTGTTATTTGTGGAGC+AGG 0.469342 2.3:+63064627 MS.gene048211:CDS
GGTACCGATATAGAAGTATT+CGG 0.469425 2.3:+63063662 MS.gene048211:CDS
TGCTTCACAAATGACAGCAT+TGG 0.474342 2.3:-63064206 None:intergenic
GAATAACGTGTTGGGAAAGA+TGG 0.476145 2.3:+63063991 MS.gene048211:CDS
GCTGCCGAATACTTCTATAT+CGG 0.477399 2.3:-63063666 None:intergenic
TCCATGCTAACGCTCTCGTC+CGG 0.480084 2.3:-63064556 None:intergenic
AAGGTACATCCGAATATGAT+TGG 0.489420 2.3:+63063864 MS.gene048211:CDS
GTGAAGGCTACCATTTGAGT+TGG 0.490964 2.3:-63063696 None:intergenic
GGCTACCGTTTAAGTTGGAC+TGG 0.492004 2.3:-63063835 None:intergenic
ACCTAACGGCGATACATTTG+TGG 0.492427 2.3:-63063567 None:intergenic
TTATGTGCACATCTTCTGCT+TGG 0.493993 2.3:+63065010 MS.gene048211:CDS
CTGTCGATGATATCTGAATT+GGG 0.497389 2.3:-63063809 None:intergenic
AAGAGCAAGGGTGGAATCAT+CGG 0.498333 2.3:+63064069 MS.gene048211:CDS
AGCTGTCTGTCCAACTCAAA+TGG 0.502650 2.3:+63063686 MS.gene048211:CDS
GGAAAGATGGTATCGGTTGG+CGG 0.502999 2.3:+63064004 MS.gene048211:CDS
GGATTCATGAATAACGTGTT+GGG 0.509988 2.3:+63063983 MS.gene048211:CDS
AGGATTCATGAATAACGTGT+TGG 0.512384 2.3:+63063982 MS.gene048211:CDS
AGAGCTAGGCTATAAGGAAT+CGG 0.514017 2.3:+63063769 MS.gene048211:CDS
GTGGAGGCTACCGTTTAAGT+TGG 0.523399 2.3:-63063840 None:intergenic
ATTCTCTTCTTATCATCTCT+AGG 0.523465 2.3:-63064742 None:intergenic
GATTCTCCTTCATCTTCTAG+TGG 0.524436 2.3:+63063479 MS.gene048211:CDS
CATTATCTTGAGATGTACCT+TGG 0.526204 2.3:-63063718 None:intergenic
GACATGAACTGTTTGGAAAG+TGG 0.527553 2.3:+63064839 MS.gene048211:CDS
CAAAAGAAACGTAGTTCAGC+CGG 0.533280 2.3:+63063263 MS.gene048211:CDS
GCTTGTTTCCAAAGAAACAT+TGG 0.537666 2.3:-63063393 None:intergenic
GTTAGGTGCTTACATGTTGG+AGG 0.539755 2.3:+63064042 MS.gene048211:CDS
GGTATGTGATGCAAAGCAAA+AGG 0.541821 2.3:-63064535 None:intergenic
ACCGGACGAGAGCGTTAGCA+TGG 0.542781 2.3:+63064555 MS.gene048211:CDS
GACTGTTGCTGGTAGTATAA+CGG 0.543849 2.3:-63063290 None:intergenic
CATGCTCTCAAGCATAAACC+TGG 0.546708 2.3:+63064301 MS.gene048211:CDS
CGAACGAACGGAGGTCCGCC+AGG 0.546839 2.3:-63064319 None:intergenic
AAGGTTAGGTGCTTACATGT+TGG 0.546985 2.3:+63064039 MS.gene048211:CDS
TTGGCGGTTCTCCAATTCAA+AGG 0.547368 2.3:+63064020 MS.gene048211:CDS
TTCTTTGGAAACAAGCAACT+GGG 0.548975 2.3:+63063400 MS.gene048211:CDS
TTCAAAGTCTCAGCAAACCT+TGG 0.553167 2.3:-63064679 None:intergenic
TCAATCACCGAGGTACTCAA+TGG 0.553423 2.3:-63064898 None:intergenic
CTGGTAGTATAACGGTATAC+CGG 0.553841 2.3:-63063282 None:intergenic
CTTGAGATGTACCTTGGTGA+AGG 0.559050 2.3:-63063712 None:intergenic
TCTTCACCAGTCTCAAAAGC+CGG 0.563592 2.3:-63064591 None:intergenic
TACATTTGTGGTGTTGTCAG+AGG 0.565214 2.3:-63063555 None:intergenic
GGAGGGATTAAGAGCAAGGG+TGG 0.565223 2.3:+63064060 MS.gene048211:CDS
ACCACAAATGTATCGCCGTT+AGG 0.567915 2.3:+63063566 MS.gene048211:CDS
GAGTCATAGTGAGAAAATTG+TGG 0.570992 2.3:-63063533 None:intergenic
CATATTCGGATGTACCTTGG+TGG 0.571085 2.3:-63063859 None:intergenic
GCATGGAGAATCATAGAGAC+CGG 0.573064 2.3:+63064572 MS.gene048211:CDS
CAATCGTTTCATGCTAGAGG+TGG 0.577074 2.3:+63064364 MS.gene048211:CDS
AATCATATTCGGATGTACCT+TGG 0.577241 2.3:-63063862 None:intergenic
TGTTGGAGGGATTAAGAGCA+AGG 0.578699 2.3:+63064056 MS.gene048211:CDS
TCACAATCGTTTCATGCTAG+AGG 0.578887 2.3:+63064361 MS.gene048211:CDS
GAATTAATGATTTCAGAGCT+AGG 0.580583 2.3:+63063755 MS.gene048211:CDS
TTAGGTGCTTACATGTTGGA+GGG 0.580819 2.3:+63064043 MS.gene048211:CDS
TCCACAAATAACACGACCTT+AGG 0.582649 2.3:-63064622 None:intergenic
GCATTACCACTAGAAGATGA+AGG 0.589681 2.3:-63063485 None:intergenic
GTGGTAATGCATCCAACACC+AGG 0.589942 2.3:+63063498 MS.gene048211:CDS
CGTTAACATCATAGCTTGTG+AGG 0.593625 2.3:+63064798 MS.gene048211:CDS
ATTCGGATGTACCTTGGTGG+AGG 0.599110 2.3:-63063856 None:intergenic
CGTGTTGGGAAAGATGGTAT+CGG 0.599692 2.3:+63063997 MS.gene048211:CDS
TTGGGAAAGATGGTATCGGT+TGG 0.601702 2.3:+63064001 MS.gene048211:CDS
GGCTACTTGAGAAGATTCCA+AGG 0.613501 2.3:-63063438 None:intergenic
GTTAACATCATAGCTTGTGA+GGG 0.618537 2.3:+63064799 MS.gene048211:CDS
ACCGTTACCATTGAGTACCT+CGG 0.638018 2.3:+63064891 MS.gene048211:CDS
GCACTAGAGTAAACACCTAA+CGG 0.650576 2.3:-63063581 None:intergenic
ACCGAGGTACTCAATGGTAA+CGG 0.653014 2.3:-63064892 None:intergenic
ATTGACTTCCAAATTGCACA+AGG 0.655189 2.3:+63064256 MS.gene048211:CDS
GTTAGGTGTTTACTCTAGTG+CGG 0.655711 2.3:+63063583 MS.gene048211:CDS
ATGAACTGTTTGGAAAGTGG+AGG 0.656082 2.3:+63064842 MS.gene048211:CDS
TGTTAAAATGTATGGATGTG+AGG 0.656156 2.3:+63064459 MS.gene048211:CDS
GTTGGAGGGATTAAGAGCAA+GGG 0.658974 2.3:+63064057 MS.gene048211:CDS
ATGTTGCTATCAATTTAGCG+TGG 0.662028 2.3:+63064977 MS.gene048211:CDS
AATGTTTGAATCAATTGATG+TGG 0.662206 2.3:+63064714 MS.gene048211:CDS
ATACCTGTTACACGAACGAA+CGG 0.677244 2.3:-63064331 None:intergenic
CCTGTTACACGAACGAACGG+AGG 0.680914 2.3:-63064328 None:intergenic
GTTTACTCTAGTGCGGACGA+TGG 0.696726 2.3:+63063590 MS.gene048211:CDS
TAATGCAGAACAACACTGTG+TGG 0.712966 2.3:+63064765 MS.gene048211:CDS
GCTCTCAAGCATAAACCTGG+CGG 0.733186 2.3:+63064304 MS.gene048211:CDS
TCAAATGGTAGCCTTCACCA+AGG 0.752690 2.3:+63063701 MS.gene048211:CDS
GAGCAAGGGTGGAATCATCG+GGG 0.766989 2.3:+63064071 MS.gene048211:CDS
TTAAACGGTAGCCTCCACCA+AGG 0.778392 2.3:+63063845 MS.gene048211:CDS
TCTAACTGAATCAATCACCG+AGG 0.797585 2.3:-63064908 None:intergenic
AAATTGCACAAGGATCACAG+TGG 0.820097 2.3:+63064266 MS.gene048211:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTTCAATAAAGATTAT+AGG + chr2.3:63064941-63064960 MS.gene048211:CDS 15.0%
!! ATGTAAGCAAATTGAAAATA+TGG - chr2.3:63064181-63064200 None:intergenic 20.0%
!! TGTAAGCAAATTGAAAATAT+GGG - chr2.3:63064180-63064199 None:intergenic 20.0%
!! TTCAATAGTGTTAAAATGTA+TGG + chr2.3:63064451-63064470 MS.gene048211:CDS 20.0%
!! TTGCAAATCAATTCAAATAA+TGG + chr2.3:63063353-63063372 MS.gene048211:CDS 20.0%
! AAAGCTCAATTTCTTTCATT+TGG - chr2.3:63063634-63063653 None:intergenic 25.0%
! AATGTTTGAATCAATTGATG+TGG + chr2.3:63064714-63064733 MS.gene048211:CDS 25.0%
! ATCAAAAGCTATGAATTCAT+TGG - chr2.3:63063462-63063481 None:intergenic 25.0%
! TTAACACTATTGAACTCAAA+TGG - chr2.3:63064445-63064464 None:intergenic 25.0%
!! AATTTGAGACCAATCATATT+CGG - chr2.3:63063876-63063895 None:intergenic 25.0%
ATTCTCTTCTTATCATCTCT+AGG - chr2.3:63064745-63064764 None:intergenic 30.0%
ATTGAAAGACATGAACTGTT+TGG + chr2.3:63064832-63064851 MS.gene048211:CDS 30.0%
GAAAGAAATTGAGCTTTCAT+TGG + chr2.3:63063637-63063656 MS.gene048211:CDS 30.0%
GAATTAATGATTTCAGAGCT+AGG + chr2.3:63063755-63063774 MS.gene048211:CDS 30.0%
TCAAAGAACCAATGTTTCTT+TGG + chr2.3:63063385-63063404 MS.gene048211:CDS 30.0%
TGAGAAAATTGTGGTGAAAA+AGG - chr2.3:63063527-63063546 None:intergenic 30.0%
TGTTAAAATGTATGGATGTG+AGG + chr2.3:63064459-63064478 MS.gene048211:CDS 30.0%
! GGTGATTTTCAAAAAGCAAT+AGG + chr2.3:63063962-63063981 MS.gene048211:CDS 30.0%
! TGTGCACAGTTTGTATTTTA+TGG + chr2.3:63063941-63063960 MS.gene048211:CDS 30.0%
AAGGTACATCCGAATATGAT+TGG + chr2.3:63063864-63063883 MS.gene048211:CDS 35.0%
AATCATATTCGGATGTACCT+TGG - chr2.3:63063865-63063884 None:intergenic 35.0%
AGGATTCATGAATAACGTGT+TGG + chr2.3:63063982-63064001 MS.gene048211:CDS 35.0%
ATGTTGCTATCAATTTAGCG+TGG + chr2.3:63064977-63064996 MS.gene048211:CDS 35.0%
CATTATCTTGAGATGTACCT+TGG - chr2.3:63063721-63063740 None:intergenic 35.0%
CTGTCGATGATATCTGAATT+GGG - chr2.3:63063812-63063831 None:intergenic 35.0%
GAAATTGAGCTTTCATTGGT+AGG + chr2.3:63063641-63063660 MS.gene048211:CDS 35.0%
GAATCGGAGATTTCATTGTT+AGG + chr2.3:63063785-63063804 MS.gene048211:CDS 35.0%
GAGTCATAGTGAGAAAATTG+TGG - chr2.3:63063536-63063555 None:intergenic 35.0%
GCTTGTTTCCAAAGAAACAT+TGG - chr2.3:63063396-63063415 None:intergenic 35.0%
GGATTCATGAATAACGTGTT+GGG + chr2.3:63063983-63064002 MS.gene048211:CDS 35.0%
TAAGCACCTAACCTTTGAAT+TGG - chr2.3:63064034-63064053 None:intergenic 35.0%
TCAGCAAACCTTGGTAAAAA+TGG - chr2.3:63064673-63064692 None:intergenic 35.0%
! AGGAAAAGATTTTCAATGGC+GGG + chr2.3:63064862-63064881 MS.gene048211:CDS 35.0%
! ATTGACTTCCAAATTGCACA+AGG + chr2.3:63064256-63064275 MS.gene048211:CDS 35.0%
! GAGGAAAAGATTTTCAATGG+CGG + chr2.3:63064861-63064880 MS.gene048211:CDS 35.0%
! GTGGAGGAAAAGATTTTCAA+TGG + chr2.3:63064858-63064877 MS.gene048211:CDS 35.0%
! GTTAACATCATAGCTTGTGA+GGG + chr2.3:63064799-63064818 MS.gene048211:CDS 35.0%
! TCTTCTTTCAAGTCTAGCTT+TGG - chr2.3:63063911-63063930 None:intergenic 35.0%
! TTCTTTGGAAACAAGCAACT+GGG + chr2.3:63063400-63063419 MS.gene048211:CDS 35.0%
! TTTCTTTGGAAACAAGCAAC+TGG + chr2.3:63063399-63063418 MS.gene048211:CDS 35.0%
! TTTTGCTTTGCATCACATAC+CGG + chr2.3:63064537-63064556 MS.gene048211:CDS 35.0%
!! ACACATCTCCATTTTTACCA+AGG + chr2.3:63064662-63064681 MS.gene048211:CDS 35.0%
!! GGTACCGATATAGAAGTATT+CGG + chr2.3:63063662-63063681 MS.gene048211:CDS 35.0%
AAATTGCACAAGGATCACAG+TGG + chr2.3:63064266-63064285 MS.gene048211:CDS 40.0%
AAGGTTAGGTGCTTACATGT+TGG + chr2.3:63064039-63064058 MS.gene048211:CDS 40.0%
ACTGTGATCCTTGTGCAATT+TGG - chr2.3:63064267-63064286 None:intergenic 40.0%
AGAGCTAGGCTATAAGGAAT+CGG + chr2.3:63063769-63063788 MS.gene048211:CDS 40.0%
AGCTTCCAGTCCAACTTAAA+CGG + chr2.3:63063830-63063849 MS.gene048211:CDS 40.0%
ATACCTGTTACACGAACGAA+CGG - chr2.3:63064334-63064353 None:intergenic 40.0%
ATGAACTGTTTGGAAAGTGG+AGG + chr2.3:63064842-63064861 MS.gene048211:CDS 40.0%
CAAAAGAAACGTAGTTCAGC+CGG + chr2.3:63063263-63063282 MS.gene048211:CDS 40.0%
CTCATCAACTCATTGCTTGT+TGG - chr2.3:63064130-63064149 None:intergenic 40.0%
CTGGTAGTATAACGGTATAC+CGG - chr2.3:63063285-63063304 None:intergenic 40.0%
GAATAACGTGTTGGGAAAGA+TGG + chr2.3:63063991-63064010 MS.gene048211:CDS 40.0%
GACATGAACTGTTTGGAAAG+TGG + chr2.3:63064839-63064858 MS.gene048211:CDS 40.0%
GACTGTTGCTGGTAGTATAA+CGG - chr2.3:63063293-63063312 None:intergenic 40.0%
GATTCTCCTTCATCTTCTAG+TGG + chr2.3:63063479-63063498 MS.gene048211:CDS 40.0%
GATTTCAGAGCTAGGCTATA+AGG + chr2.3:63063763-63063782 MS.gene048211:CDS 40.0%
GCACTAGAGTAAACACCTAA+CGG - chr2.3:63063584-63063603 None:intergenic 40.0%
GCATTACCACTAGAAGATGA+AGG - chr2.3:63063488-63063507 None:intergenic 40.0%
GCTGCCGAATACTTCTATAT+CGG - chr2.3:63063669-63063688 None:intergenic 40.0%
GCTGTCGATGATATCTGAAT+TGG - chr2.3:63063813-63063832 None:intergenic 40.0%
GGTATGTGATGCAAAGCAAA+AGG - chr2.3:63064538-63064557 None:intergenic 40.0%
GGTGGAAGACTTGATATTGT+AGG + chr2.3:63064382-63064401 MS.gene048211:CDS 40.0%
GGTTCTCCAATTCAAAGGTT+AGG + chr2.3:63064025-63064044 MS.gene048211:CDS 40.0%
GTTAGGTGTTTACTCTAGTG+CGG + chr2.3:63063583-63063602 MS.gene048211:CDS 40.0%
TAATGCAGAACAACACTGTG+TGG + chr2.3:63064765-63064784 MS.gene048211:CDS 40.0%
TCACAATCGTTTCATGCTAG+AGG + chr2.3:63064361-63064380 MS.gene048211:CDS 40.0%
TCCACAAATAACACGACCTT+AGG - chr2.3:63064625-63064644 None:intergenic 40.0%
TCTAACTGAATCAATCACCG+AGG - chr2.3:63064911-63064930 None:intergenic 40.0%
TGAAAAAGGATTCCTGGTGT+TGG - chr2.3:63063513-63063532 None:intergenic 40.0%
TGCTTCACAAATGACAGCAT+TGG - chr2.3:63064209-63064228 None:intergenic 40.0%
TTAGGTGCTTACATGTTGGA+GGG + chr2.3:63064043-63064062 MS.gene048211:CDS 40.0%
TTATGTGCACATCTTCTGCT+TGG + chr2.3:63065010-63065029 MS.gene048211:CDS 40.0%
TTCAAAGTCTCAGCAAACCT+TGG - chr2.3:63064682-63064701 None:intergenic 40.0%
TTGTGGTGAAAAAGGATTCC+TGG - chr2.3:63063519-63063538 None:intergenic 40.0%
! CGTTAACATCATAGCTTGTG+AGG + chr2.3:63064798-63064817 MS.gene048211:CDS 40.0%
! GGAGAAGCAATTTTGTACCT+TGG + chr2.3:63063421-63063440 MS.gene048211:CDS 40.0%
!! TACATTTGTGGTGTTGTCAG+AGG - chr2.3:63063558-63063577 None:intergenic 40.0%
AAGAGCAAGGGTGGAATCAT+CGG + chr2.3:63064069-63064088 MS.gene048211:CDS 45.0%
ACCACAAATGTATCGCCGTT+AGG + chr2.3:63063566-63063585 MS.gene048211:CDS 45.0%
ACCGTTACCATTGAGTACCT+CGG + chr2.3:63064891-63064910 MS.gene048211:CDS 45.0%
ACCTAACGGCGATACATTTG+TGG - chr2.3:63063570-63063589 None:intergenic 45.0%
AGCTGTCTGTCCAACTCAAA+TGG + chr2.3:63063686-63063705 MS.gene048211:CDS 45.0%
CAATCGTTTCATGCTAGAGG+TGG + chr2.3:63064364-63064383 MS.gene048211:CDS 45.0%
CATATTCGGATGTACCTTGG+TGG - chr2.3:63063862-63063881 None:intergenic 45.0%
CATGCTCTCAAGCATAAACC+TGG + chr2.3:63064301-63064320 MS.gene048211:CDS 45.0%
CGTGTTGGGAAAGATGGTAT+CGG + chr2.3:63063997-63064016 MS.gene048211:CDS 45.0%
CTTGAGATGTACCTTGGTGA+AGG - chr2.3:63063715-63063734 None:intergenic 45.0%
GCATGGAGAATCATAGAGAC+CGG + chr2.3:63064572-63064591 MS.gene048211:CDS 45.0%
GGCTACTTGAGAAGATTCCA+AGG - chr2.3:63063441-63063460 None:intergenic 45.0%
GTATGGATGTGAGGTTCAAC+TGG + chr2.3:63064468-63064487 MS.gene048211:CDS 45.0%
GTGAAGGCTACCATTTGAGT+TGG - chr2.3:63063699-63063718 None:intergenic 45.0%
GTTAGGTGCTTACATGTTGG+AGG + chr2.3:63064042-63064061 MS.gene048211:CDS 45.0%
GTTGGAGGGATTAAGAGCAA+GGG + chr2.3:63064057-63064076 MS.gene048211:CDS 45.0%
TCAAATGGTAGCCTTCACCA+AGG + chr2.3:63063701-63063720 MS.gene048211:CDS 45.0%
TCTGTCTCAAAGACTGTTGC+TGG - chr2.3:63063304-63063323 None:intergenic 45.0%
TCTTCACCAGTCTCAAAAGC+CGG - chr2.3:63064594-63064613 None:intergenic 45.0%
TGTTGGAGGGATTAAGAGCA+AGG + chr2.3:63064056-63064075 MS.gene048211:CDS 45.0%
TTGGCGGTTCTCCAATTCAA+AGG + chr2.3:63064020-63064039 MS.gene048211:CDS 45.0%
! ACCGAGGTACTCAATGGTAA+CGG - chr2.3:63064895-63064914 None:intergenic 45.0%
! GGTGAAGATTTTGTCGCCTA+AGG + chr2.3:63064606-63064625 MS.gene048211:CDS 45.0%
! TCAATCACCGAGGTACTCAA+TGG - chr2.3:63064901-63064920 None:intergenic 45.0%
!! GCCTAAGGTCGTGTTATTTG+TGG + chr2.3:63064621-63064640 MS.gene048211:CDS 45.0%
!! TTGGGAAAGATGGTATCGGT+TGG + chr2.3:63064001-63064020 MS.gene048211:CDS 45.0%
AGAGCAAGGGTGGAATCATC+GGG + chr2.3:63064070-63064089 MS.gene048211:CDS 50.0%
ATTCGGATGTACCTTGGTGG+AGG - chr2.3:63063859-63063878 None:intergenic 50.0%
GCTCTCAAGCATAAACCTGG+CGG + chr2.3:63064304-63064323 MS.gene048211:CDS 50.0%
GTGGAGGCTACCGTTTAAGT+TGG - chr2.3:63063843-63063862 None:intergenic 50.0%
GTTTACTCTAGTGCGGACGA+TGG + chr2.3:63063590-63063609 MS.gene048211:CDS 50.0%
TTAAACGGTAGCCTCCACCA+AGG + chr2.3:63063845-63063864 MS.gene048211:CDS 50.0%
! GTGGTAATGCATCCAACACC+AGG + chr2.3:63063498-63063517 MS.gene048211:CDS 50.0%
!! GGAAAGATGGTATCGGTTGG+CGG + chr2.3:63064004-63064023 MS.gene048211:CDS 50.0%
!! GGCTACCGTTTAAGTTGGAC+TGG - chr2.3:63063838-63063857 None:intergenic 50.0%
!! GGTCGTGTTATTTGTGGAGC+AGG + chr2.3:63064627-63064646 MS.gene048211:CDS 50.0%
!!! TAGAGACCGGCTTTTGAGAC+TGG + chr2.3:63064585-63064604 MS.gene048211:CDS 50.0%
CCTCCGTTCGTTCGTGTAAC+AGG + chr2.3:63064328-63064347 MS.gene048211:CDS 55.0%
CCTGTTACACGAACGAACGG+AGG - chr2.3:63064331-63064350 None:intergenic 55.0%
GAGCAAGGGTGGAATCATCG+GGG + chr2.3:63064071-63064090 MS.gene048211:CDS 55.0%
GGAGGGATTAAGAGCAAGGG+TGG + chr2.3:63064060-63064079 MS.gene048211:CDS 55.0%
TCCATGCTAACGCTCTCGTC+CGG - chr2.3:63064559-63064578 None:intergenic 55.0%
!! ACCGGACGAGAGCGTTAGCA+TGG + chr2.3:63064555-63064574 MS.gene048211:CDS 60.0%
CGAACGAACGGAGGTCCGCC+AGG - chr2.3:63064322-63064341 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 63063251 63065044 63063251 ID=MS.gene048211
chr2.3 mRNA 63063251 63065044 63063251 ID=MS.gene048211.t1;Parent=MS.gene048211
chr2.3 exon 63063251 63065044 63063251 ID=MS.gene048211.t1.exon1;Parent=MS.gene048211.t1
chr2.3 CDS 63063251 63065044 63063251 ID=cds.MS.gene048211.t1;Parent=MS.gene048211.t1
Gene Sequence

>MS.gene048211

ATGTATACTTCTCAAAAGAAACGTAGTTCAGCCGGTATACCGTTATACTACCAGCAACAGTCTTTGAGACAGATTGATTCAAACAACAATCGTTTCAAAACGTTGCAAATCAATTCAAATAATGGTAGTATCAGTCAAAGAACCAATGTTTCTTTGGAAACAAGCAACTGGGAGAAGCAATTTTGTACCTTGGAATCTTCTCAAGTAGCCAATGAATTCATAGCTTTTGATTCTCCTTCATCTTCTAGTGGTAATGCATCCAACACCAGGAATCCTTTTTCACCACAATTTTCTCACTATGACTCCTCTGACAACACCACAAATGTATCGCCGTTAGGTGTTTACTCTAGTGCGGACGATGGTAGCTTTGAATTGAAGCACCAAATGAAAGAAATTGAGCTTTCATTGGTAGGTACCGATATAGAAGTATTCGGCAGCTGTCTGTCCAACTCAAATGGTAGCCTTCACCAAGGTACATCTCAAGATAATGAGTTTCAAATTGATGAATTAATGATTTCAGAGCTAGGCTATAAGGAATCGGAGATTTCATTGTTAGGTCCCAATTCAGATATCATCGACAGCTTCCAGTCCAACTTAAACGGTAGCCTCCACCAAGGTACATCCGAATATGATTGGTCTCAAATTGTAGAAATGATTCCAAAGCTAGACTTGAAAGAAGAACTTATTAGATGTGCACAGTTTGTATTTTATGGTGATTTTCAAAAAGCAATAGGATTCATGAATAACGTGTTGGGAAAGATGGTATCGGTTGGCGGTTCTCCAATTCAAAGGTTAGGTGCTTACATGTTGGAGGGATTAAGAGCAAGGGTGGAATCATCGGGGAGTGCAATTTATAATGCGTTGAAATGTGAAGAACCAACAAGCAATGAGTTGATGAGTGCTATGCACACTTTGTATCAGATTTGCCCATATTTTCAATTTGCTTACATATCATCCAATGCTGTCATTTGTGAAGCAATGCAAAATGAATCAAGAATTCATATAATTGACTTCCAAATTGCACAAGGATCACAGTGGATGTTACTTTTGCATGCTCTCAAGCATAAACCTGGCGGACCTCCGTTCGTTCGTGTAACAGGTATCGATGATTCACAATCGTTTCATGCTAGAGGTGGAAGACTTGATATTGTAGGAAAAAAGCTAGAAGATTGTGCAAAAACTTGTAGAGTTCCATTTGAGTTCAATAGTGTTAAAATGTATGGATGTGAGGTTCAACTGGAAGATTTTGAAGTTCAACATGATGAAGTTCTTGCTGTGAATTTTCCTTTTGCTTTGCATCACATACCGGACGAGAGCGTTAGCATGGAGAATCATAGAGACCGGCTTTTGAGACTGGTGAAGATTTTGTCGCCTAAGGTCGTGTTATTTGTGGAGCAGGAGTCAAACACAAACACATCTCCATTTTTACCAAGGTTTGCTGAGACTTTGAATTATTACACTGCAATGTTTGAATCAATTGATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAGCTTGTGAGGGAGATGAGAGAATTGAAAGACATGAACTGTTTGGAAAGTGGAGGAAAAGATTTTCAATGGCGGGTTTTGTACCGTTACCATTGAGTACCTCGGTGATTGATTCAGTTAGAACTCTCTTGAAAGATTTCAATAAAGATTATAGGATTGAAGAAACAGATGTTGCTATCAATTTAGCGTGGAAGAGTAAAGTTATGTGCACATCTTCTGCTTGGAGATGTTATTGA
Protein sequence

>MS.gene048211.t1

MYTSQKKRSSAGIPLYYQQQSLRQIDSNNNRFKTLQINSNNGSISQRTNVSLETSNWEKQFCTLESSQVANEFIAFDSPSSSSGNASNTRNPFSPQFSHYDSSDNTTNVSPLGVYSSADDGSFELKHQMKEIELSLVGTDIEVFGSCLSNSNGSLHQGTSQDNEFQIDELMISELGYKESEISLLGPNSDIIDSFQSNLNGSLHQGTSEYDWSQIVEMIPKLDLKEELIRCAQFVFYGDFQKAIGFMNNVLGKMVSVGGSPIQRLGAYMLEGLRARVESSGSAIYNALKCEEPTSNELMSAMHTLYQICPYFQFAYISSNAVICEAMQNESRIHIIDFQIAQGSQWMLLLHALKHKPGGPPFVRVTGIDDSQSFHARGGRLDIVGKKLEDCAKTCRVPFEFNSVKMYGCEVQLEDFEVQHDEVLAVNFPFALHHIPDESVSMENHRDRLLRLVKILSPKVVLFVEQESNTNTSPFLPRFAETLNYYTAMFESIDVALPRDDKKRINAEQHCVARDIVNIIACEGDERIERHELFGKWRKRFSMAGFVPLPLSTSVIDSVRTLLKDFNKDYRIEETDVAINLAWKSKVMCTSSAWRCY