Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048333.t1 | AET03392.2 | 93 | 242 | 16 | 1 | 258 | 498 | 13 | 254 | 1.90E-127 | 466.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048333.t1 | Q8S8F8 | 68.6 | 236 | 67 | 2 | 2 | 233 | 67 | 299 | 3.5e-85 | 317.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048333.t1 | G7LJ96 | 93.0 | 242 | 16 | 1 | 258 | 498 | 13 | 254 | 1.4e-127 | 466.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene048333.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048333.t1 | MTR_8g069110 | 99.563 | 229 | 1 | 0 | 1 | 229 | 43 | 271 | 4.44e-168 | 479 |
| MS.gene048333.t1 | MTR_8g069080 | 92.975 | 242 | 16 | 1 | 258 | 498 | 13 | 254 | 6.74e-151 | 436 |
| MS.gene048333.t1 | MTR_2g076780 | 91.480 | 223 | 18 | 1 | 277 | 498 | 1 | 223 | 3.76e-139 | 404 |
| MS.gene048333.t1 | MTR_3g100750 | 65.714 | 210 | 66 | 2 | 18 | 227 | 54 | 257 | 9.42e-88 | 273 |
| MS.gene048333.t1 | MTR_7g083570 | 47.500 | 160 | 83 | 1 | 67 | 225 | 115 | 274 | 2.36e-48 | 171 |
| MS.gene048333.t1 | MTR_2g076350 | 42.478 | 226 | 122 | 5 | 12 | 232 | 10 | 232 | 1.41e-47 | 167 |
| MS.gene048333.t1 | MTR_5g032600 | 45.213 | 188 | 94 | 2 | 308 | 493 | 73 | 253 | 2.09e-42 | 154 |
| MS.gene048333.t1 | MTR_1g033750 | 35.644 | 202 | 114 | 3 | 25 | 225 | 34 | 220 | 6.43e-37 | 137 |
| MS.gene048333.t1 | MTR_1g114640 | 36.813 | 182 | 102 | 3 | 46 | 225 | 48 | 218 | 6.53e-36 | 134 |
| MS.gene048333.t1 | MTR_6g077990 | 39.623 | 159 | 95 | 1 | 68 | 225 | 37 | 195 | 2.25e-35 | 132 |
| MS.gene048333.t1 | MTR_6g077990 | 39.623 | 159 | 95 | 1 | 68 | 225 | 38 | 196 | 2.27e-35 | 132 |
| MS.gene048333.t1 | MTR_8g100065 | 38.509 | 161 | 97 | 1 | 67 | 225 | 22 | 182 | 2.60e-35 | 132 |
| MS.gene048333.t1 | MTR_8g100065 | 38.509 | 161 | 97 | 1 | 67 | 225 | 44 | 204 | 6.71e-35 | 131 |
| MS.gene048333.t1 | MTR_1g114690 | 37.107 | 159 | 99 | 1 | 68 | 225 | 57 | 215 | 8.06e-35 | 131 |
| MS.gene048333.t1 | MTR_1g077020 | 38.071 | 197 | 87 | 4 | 312 | 501 | 8 | 176 | 3.92e-34 | 128 |
| MS.gene048333.t1 | MTR_5g020510 | 37.563 | 197 | 99 | 5 | 313 | 498 | 99 | 282 | 5.17e-34 | 131 |
| MS.gene048333.t1 | MTR_6g077990 | 41.096 | 146 | 85 | 1 | 81 | 225 | 8 | 153 | 1.00e-33 | 126 |
| MS.gene048333.t1 | MTR_1g114690 | 35.849 | 159 | 101 | 1 | 68 | 225 | 57 | 215 | 2.74e-33 | 127 |
| MS.gene048333.t1 | MTR_4g134580 | 35.111 | 225 | 105 | 6 | 312 | 498 | 112 | 333 | 8.78e-32 | 127 |
| MS.gene048333.t1 | MTR_3g100650 | 42.703 | 185 | 95 | 3 | 312 | 493 | 79 | 255 | 4.15e-31 | 122 |
| MS.gene048333.t1 | MTR_1g114750 | 33.750 | 160 | 105 | 1 | 67 | 225 | 59 | 218 | 6.72e-31 | 120 |
| MS.gene048333.t1 | MTR_1g114690 | 30.570 | 193 | 99 | 2 | 68 | 225 | 57 | 249 | 4.67e-29 | 116 |
| MS.gene048333.t1 | MTR_7g010300 | 38.421 | 190 | 93 | 6 | 313 | 492 | 88 | 263 | 6.26e-28 | 115 |
| MS.gene048333.t1 | MTR_1g115130 | 34.932 | 146 | 94 | 1 | 81 | 225 | 46 | 191 | 1.14e-27 | 110 |
| MS.gene048333.t1 | MTR_8g442330 | 38.798 | 183 | 55 | 5 | 309 | 486 | 9 | 139 | 4.26e-27 | 107 |
| MS.gene048333.t1 | MTR_6g012280 | 39.891 | 183 | 99 | 5 | 313 | 492 | 91 | 265 | 6.99e-26 | 109 |
| MS.gene048333.t1 | MTR_1g114680 | 43.636 | 110 | 61 | 1 | 121 | 229 | 55 | 164 | 2.79e-22 | 95.1 |
| MS.gene048333.t1 | MTR_8g069380 | 57.143 | 84 | 27 | 3 | 419 | 498 | 23 | 101 | 6.38e-19 | 83.2 |
| MS.gene048333.t1 | MTR_5g065440 | 47.059 | 85 | 40 | 1 | 283 | 362 | 23 | 107 | 9.47e-18 | 84.0 |
| MS.gene048333.t1 | MTR_6g077990 | 36.111 | 108 | 68 | 1 | 68 | 174 | 37 | 144 | 9.59e-18 | 80.9 |
| MS.gene048333.t1 | MTR_8g100065 | 36.000 | 100 | 64 | 0 | 67 | 166 | 44 | 143 | 1.58e-17 | 80.5 |
| MS.gene048333.t1 | MTR_8g100065 | 36.000 | 100 | 64 | 0 | 67 | 166 | 22 | 121 | 1.72e-17 | 79.7 |
| MS.gene048333.t1 | MTR_3g039810 | 62.745 | 51 | 19 | 0 | 312 | 362 | 19 | 69 | 1.73e-17 | 78.2 |
| MS.gene048333.t1 | MTR_8g051480 | 52.874 | 87 | 32 | 5 | 420 | 501 | 36 | 118 | 1.60e-16 | 76.6 |
| MS.gene048333.t1 | MTR_1g114640 | 32.331 | 133 | 77 | 3 | 46 | 176 | 48 | 169 | 1.91e-15 | 75.1 |
| MS.gene048333.t1 | MTR_1g114690 | 31.667 | 120 | 73 | 2 | 67 | 177 | 56 | 175 | 4.32e-15 | 74.7 |
| MS.gene048333.t1 | MTR_6g012220 | 58.000 | 50 | 17 | 1 | 312 | 361 | 20 | 65 | 4.73e-15 | 70.9 |
| MS.gene048333.t1 | MTR_8g467340 | 63.462 | 52 | 17 | 1 | 313 | 362 | 64 | 115 | 7.79e-15 | 77.0 |
| MS.gene048333.t1 | MTR_6g073013 | 47.368 | 57 | 29 | 1 | 313 | 368 | 19 | 75 | 1.08e-12 | 67.4 |
| MS.gene048333.t1 | MTR_8g032100 | 60.465 | 43 | 17 | 0 | 456 | 498 | 125 | 167 | 3.07e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048333.t1 | AT2G22475 | 68.644 | 236 | 67 | 2 | 2 | 233 | 67 | 299 | 3.41e-108 | 327 |
| MS.gene048333.t1 | AT1G28200 | 61.224 | 196 | 73 | 1 | 32 | 227 | 64 | 256 | 3.50e-84 | 264 |
| MS.gene048333.t1 | AT1G28200 | 61.224 | 196 | 73 | 1 | 32 | 227 | 64 | 256 | 3.50e-84 | 264 |
| MS.gene048333.t1 | AT4G40100 | 55.187 | 241 | 60 | 5 | 2 | 234 | 25 | 225 | 2.45e-78 | 248 |
| MS.gene048333.t1 | AT2G22475 | 64.607 | 178 | 56 | 2 | 2 | 175 | 67 | 241 | 1.85e-70 | 228 |
| MS.gene048333.t1 | AT5G13200 | 48.077 | 156 | 80 | 1 | 67 | 221 | 101 | 256 | 5.92e-47 | 166 |
| MS.gene048333.t1 | AT4G01600 | 44.318 | 176 | 96 | 1 | 52 | 225 | 10 | 185 | 1.09e-46 | 163 |
| MS.gene048333.t1 | AT4G01600 | 44.318 | 176 | 96 | 1 | 52 | 225 | 49 | 224 | 2.98e-46 | 163 |
| MS.gene048333.t1 | AT4G01600 | 43.182 | 176 | 93 | 2 | 52 | 225 | 49 | 219 | 6.10e-43 | 154 |
| MS.gene048333.t1 | AT2G02540 | 39.583 | 192 | 105 | 4 | 312 | 498 | 86 | 271 | 4.07e-37 | 140 |
| MS.gene048333.t1 | AT2G02540 | 39.583 | 192 | 105 | 4 | 312 | 498 | 86 | 271 | 4.07e-37 | 140 |
| MS.gene048333.t1 | AT4G24660 | 38.806 | 201 | 90 | 4 | 312 | 507 | 48 | 220 | 6.99e-37 | 137 |
| MS.gene048333.t1 | AT4G24660 | 38.947 | 190 | 88 | 3 | 312 | 501 | 48 | 209 | 1.27e-36 | 136 |
| MS.gene048333.t1 | AT5G23350 | 37.736 | 159 | 98 | 1 | 68 | 225 | 55 | 213 | 1.68e-34 | 130 |
| MS.gene048333.t1 | AT5G23370 | 38.994 | 159 | 96 | 1 | 68 | 225 | 55 | 213 | 1.85e-34 | 130 |
| MS.gene048333.t1 | AT5G65410 | 34.959 | 246 | 130 | 5 | 266 | 501 | 18 | 243 | 1.40e-33 | 130 |
| MS.gene048333.t1 | AT5G23360 | 37.107 | 159 | 99 | 1 | 68 | 225 | 47 | 205 | 5.19e-33 | 126 |
| MS.gene048333.t1 | AT5G08350 | 38.365 | 159 | 97 | 1 | 68 | 225 | 54 | 212 | 1.33e-32 | 125 |
| MS.gene048333.t1 | AT1G14440 | 38.235 | 204 | 84 | 7 | 312 | 498 | 89 | 267 | 7.67e-32 | 125 |
| MS.gene048333.t1 | AT1G14440 | 38.235 | 204 | 84 | 7 | 312 | 498 | 89 | 267 | 7.67e-32 | 125 |
| MS.gene048333.t1 | AT1G69600 | 39.037 | 187 | 91 | 6 | 313 | 493 | 31 | 200 | 1.59e-29 | 117 |
| MS.gene048333.t1 | AT2G18350 | 38.462 | 195 | 88 | 8 | 310 | 498 | 79 | 247 | 6.92e-29 | 116 |
| MS.gene048333.t1 | AT5G15210 | 30.400 | 250 | 130 | 7 | 262 | 498 | 10 | 228 | 9.62e-28 | 113 |
| MS.gene048333.t1 | AT5G13200 | 46.729 | 107 | 56 | 1 | 67 | 172 | 101 | 207 | 1.71e-27 | 110 |
| MS.gene048333.t1 | AT3G50890 | 30.342 | 234 | 118 | 7 | 280 | 501 | 33 | 233 | 1.50e-23 | 100 |
| MS.gene048333.t1 | AT1G74660 | 64.706 | 51 | 18 | 0 | 312 | 362 | 38 | 88 | 5.84e-22 | 91.3 |
| MS.gene048333.t1 | AT1G74660 | 64.706 | 51 | 18 | 0 | 312 | 362 | 38 | 88 | 1.28e-21 | 90.5 |
| MS.gene048333.t1 | AT5G60480 | 34.043 | 188 | 94 | 5 | 313 | 498 | 37 | 196 | 1.31e-20 | 91.3 |
| MS.gene048333.t1 | AT5G42780 | 32.000 | 225 | 121 | 6 | 283 | 501 | 33 | 231 | 1.84e-20 | 91.3 |
| MS.gene048333.t1 | AT1G75240 | 61.290 | 62 | 23 | 1 | 312 | 372 | 75 | 136 | 8.72e-20 | 90.9 |
| MS.gene048333.t1 | AT1G75240 | 55.357 | 56 | 25 | 0 | 446 | 501 | 237 | 292 | 3.86e-12 | 68.2 |
| MS.gene048333.t1 | AT3G28917 | 59.615 | 52 | 20 | 1 | 312 | 362 | 32 | 83 | 8.07e-18 | 79.3 |
| MS.gene048333.t1 | AT1G18835 | 58.824 | 51 | 18 | 1 | 312 | 362 | 25 | 72 | 3.63e-16 | 74.3 |
| MS.gene048333.t1 | AT3G28920 | 63.462 | 52 | 17 | 1 | 313 | 362 | 52 | 103 | 3.38e-14 | 74.3 |
Find 130 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTTAGTTTGGTCTTATTT+TGG | 0.109091 | 8.4:-34523433 | MS.gene048333:intron |
| CTGTCGACAATCATGAATTT+TGG | 0.136158 | 8.4:-34544599 | MS.gene048333:CDS |
| ACACCAGAATTACTTCTTAA+AGG | 0.178017 | 8.4:-34523318 | MS.gene048333:CDS |
| ATAAGTTTACATAGCTTATA+AGG | 0.268359 | 8.4:-34521292 | MS.gene048333:intron |
| GTTGATTCCGTAACCAATTC+AGG | 0.305826 | 8.4:+34550407 | None:intergenic |
| CTGCTTGAAGTGCATCTTGC+AGG | 0.309085 | 8.4:+34544538 | None:intergenic |
| GGGAGTCGGCTGAGGGAAAC+GGG | 0.315201 | 8.4:+34529787 | None:intergenic |
| CCTCCTCAATTCAACGTTAT+GGG | 0.323370 | 8.4:-34550314 | MS.gene048333:intron |
| TCCTCCTCAATTCAACGTTA+TGG | 0.324305 | 8.4:-34550315 | MS.gene048333:CDS |
| ATATACAAAACTCCCATTAC+TGG | 0.331546 | 8.4:+34549155 | None:intergenic |
| CACTTTCCAAGGGGTGATCT+TGG | 0.335585 | 8.4:-34529662 | MS.gene048333:CDS |
| TTTCTCTCCGATGAAAATTT+CGG | 0.335714 | 8.4:+34529855 | None:intergenic |
| GGGAAGATGGGGTAGGAAGG+TGG | 0.339032 | 8.4:-34549944 | MS.gene048333:CDS |
| TTTCCATTTGATTAATGAAC+AGG | 0.339089 | 8.4:+34530140 | None:intergenic |
| AGCATGTGACTGGGACTTCT+AGG | 0.339165 | 8.4:-34529730 | MS.gene048333:CDS |
| ACTCCTTTAAGAAGTAATTC+TGG | 0.352063 | 8.4:+34523315 | None:intergenic |
| AACAGAATGGAGTTATTATA+AGG | 0.364266 | 8.4:-34549072 | MS.gene048333:intron |
| TTGCCTGTTCATTAATCAAA+TGG | 0.365131 | 8.4:-34530143 | MS.gene048333:CDS |
| AGGGAGTCGGCTGAGGGAAA+CGG | 0.388732 | 8.4:+34529786 | None:intergenic |
| GGTACATTGGAGTCCTGAAT+TGG | 0.408737 | 8.4:-34550420 | MS.gene048333:CDS |
| ATTGTTTCAACGTAACTAGT+TGG | 0.415917 | 8.4:+34530073 | None:intergenic |
| ACCGGTTACCAGCATGTGAC+TGG | 0.421058 | 8.4:-34529740 | MS.gene048333:CDS |
| ATGGGGTAGGAAGGTGGGAG+AGG | 0.421760 | 8.4:-34549938 | MS.gene048333:CDS |
| TAGCATGGCTGAAGCTGCTA+TGG | 0.428381 | 8.4:-34549309 | MS.gene048333:CDS |
| CCCAGTCACATGCTGGTAAC+CGG | 0.435647 | 8.4:+34529739 | None:intergenic |
| TCGAACACCTGGTATTCCTC+TGG | 0.442178 | 8.4:+34523378 | None:intergenic |
| ACGTCAGCTGGTCCAGTAAT+GGG | 0.445382 | 8.4:-34549167 | MS.gene048333:CDS |
| TTCTTCATCATGATGATTCG+AGG | 0.450499 | 8.4:+34530045 | None:intergenic |
| TTCGTAATGTGCTGGGAAGA+TGG | 0.450548 | 8.4:-34549957 | MS.gene048333:CDS |
| TAAGTATCACCATGATAGAT+TGG | 0.451827 | 8.4:+34529623 | None:intergenic |
| GTCGAGAAGGGAGTCGGCTG+AGG | 0.453909 | 8.4:+34529779 | None:intergenic |
| ATTGGAGGCTACGCTCTTGA+TGG | 0.454275 | 8.4:-34529950 | MS.gene048333:CDS |
| GGAGTCGGCTGAGGGAAACG+GGG | 0.458116 | 8.4:+34529788 | None:intergenic |
| GGAAGATGGGGTAGGAAGGT+GGG | 0.458978 | 8.4:-34549943 | MS.gene048333:CDS |
| CACGTCAGCTGGTCCAGTAA+TGG | 0.459451 | 8.4:-34549168 | MS.gene048333:CDS |
| GGTGACTGTTCGTAATGTGC+TGG | 0.461322 | 8.4:-34549965 | MS.gene048333:CDS |
| TATCAAAAGGAAAGGATGCT+TGG | 0.463362 | 8.4:-34529545 | MS.gene048333:CDS |
| AAGAATCACGCTTCCAGCAT+TGG | 0.464419 | 8.4:-34529968 | MS.gene048333:CDS |
| TTGGAGTCCTGAATTGGTTA+CGG | 0.476212 | 8.4:-34550414 | MS.gene048333:CDS |
| ATAGCAGCTTCAGCCATGCT+AGG | 0.476828 | 8.4:+34549311 | None:intergenic |
| CAAGTTCACTCATTATCAAA+AGG | 0.480127 | 8.4:-34529558 | MS.gene048333:CDS |
| ATGATGAGGCCCACTTTCCA+AGG | 0.481132 | 8.4:-34529673 | MS.gene048333:CDS |
| GCCCGGTAGCATGAAGAAAT+CGG | 0.481425 | 8.4:-34550441 | None:intergenic |
| TATCATGGTGATACTTATAG+TGG | 0.482130 | 8.4:-34529617 | MS.gene048333:CDS |
| AAGATCAATTGCTGACGACT+TGG | 0.484044 | 8.4:+34521220 | None:intergenic |
| TTCTTCAGTTGTTCATGATG+AGG | 0.486893 | 8.4:-34529687 | MS.gene048333:CDS |
| AGACTCTCGCTGGAAATACT+TGG | 0.494538 | 8.4:-34549900 | MS.gene048333:CDS |
| ATTACTTCTTAAAGGAGTTG+TGG | 0.496405 | 8.4:-34523310 | MS.gene048333:CDS |
| CCTCATTGCAACAAATGTTA+CGG | 0.499846 | 8.4:-34521253 | MS.gene048333:CDS |
| AAGAGCGTAGCCTCCAATGC+TGG | 0.502391 | 8.4:+34529955 | None:intergenic |
| GGATTGGCTGTATTAGATGA+AGG | 0.504721 | 8.4:+34544648 | None:intergenic |
| GCATGTGACTGGGACTTCTA+GGG | 0.505358 | 8.4:-34529729 | MS.gene048333:CDS |
| TGCTATGGGAAGAATTGCGC+AGG | 0.506994 | 8.4:-34549294 | MS.gene048333:CDS |
| CAAGATCACCCCTTGGAAAG+TGG | 0.509725 | 8.4:+34529663 | None:intergenic |
| GTGGATGCATACGTTAGAAA+AGG | 0.511920 | 8.4:-34523291 | MS.gene048333:CDS |
| CGAACACCTGGTATTCCTCT+GGG | 0.513723 | 8.4:+34523379 | None:intergenic |
| TGTTATCTATCCACGTCAGC+TGG | 0.514433 | 8.4:-34549179 | MS.gene048333:CDS |
| GCTGGGAAGATGGGGTAGGA+AGG | 0.523116 | 8.4:-34549947 | MS.gene048333:CDS |
| AATGGAAACAAAAGAAAATG+AGG | 0.526367 | 8.4:-34530125 | MS.gene048333:CDS |
| AATCACGCTTCCAGCATTGG+AGG | 0.528529 | 8.4:-34529965 | MS.gene048333:CDS |
| TCATCATGAACAACTGAAGA+AGG | 0.530900 | 8.4:+34529689 | None:intergenic |
| CTGCAAGATGCACTTCAAGC+AGG | 0.532744 | 8.4:-34544537 | MS.gene048333:CDS |
| AAGATCACCCCTTGGAAAGT+GGG | 0.533623 | 8.4:+34529664 | None:intergenic |
| TCGTAATGTGCTGGGAAGAT+GGG | 0.534568 | 8.4:-34549956 | MS.gene048333:CDS |
| GGCTACGCTCTTGATGGCTG+TGG | 0.535093 | 8.4:-34529944 | MS.gene048333:CDS |
| CAGAACCCCAGAGGAATACC+AGG | 0.539229 | 8.4:-34523385 | MS.gene048333:CDS |
| GGAGGAGGTTGAGAGAAAGA+AGG | 0.541026 | 8.4:+34550332 | None:intergenic |
| GAGTCGGCTGAGGGAAACGG+GGG | 0.542032 | 8.4:+34529789 | None:intergenic |
| GGTGGGAGAGGCAACTAAGA+AGG | 0.542243 | 8.4:-34549926 | MS.gene048333:CDS |
| CTCAAGTCTTCTTTATACAC+GGG | 0.545365 | 8.4:+34523345 | None:intergenic |
| TTGACACTGTTCCAGAAGAA+CGG | 0.550516 | 8.4:-34549220 | MS.gene048333:CDS |
| CCAAGGGGTGATCTTGGAGA+AGG | 0.555872 | 8.4:-34529656 | MS.gene048333:CDS |
| GATACTTATAGTGGTGGAGA+AGG | 0.556865 | 8.4:-34529608 | MS.gene048333:CDS |
| TAGCAGCTTCAGCCATGCTA+GGG | 0.556987 | 8.4:+34549312 | None:intergenic |
| TCTCATATCCACCTTCTGCT+AGG | 0.557742 | 8.4:+34549261 | None:intergenic |
| TTCTGACTTTGAAGATGAGG+AGG | 0.558370 | 8.4:-34523226 | MS.gene048333:CDS |
| GTCGAAGAAGAAGAAGCAAA+AGG | 0.559749 | 8.4:-34523267 | MS.gene048333:CDS |
| TGATGAGGCCCACTTTCCAA+GGG | 0.559791 | 8.4:-34529672 | MS.gene048333:CDS |
| TAGAAGTCCCAGTCACATGC+TGG | 0.560832 | 8.4:+34529732 | None:intergenic |
| GATTGTCGACAGAGATCACT+TGG | 0.563642 | 8.4:+34544610 | None:intergenic |
| TTGAACTATGATAACGCTGT+GGG | 0.567844 | 8.4:-34544564 | MS.gene048333:CDS |
| AGATTCTGACTTTGAAGATG+AGG | 0.569381 | 8.4:-34523229 | MS.gene048333:CDS |
| ACTCAAGTCTTCTTTATACA+CGG | 0.571094 | 8.4:+34523344 | None:intergenic |
| TCACTCATTATCAAAAGGAA+AGG | 0.573122 | 8.4:-34529553 | MS.gene048333:CDS |
| GCATGAAGAAATCGGTACAT+TGG | 0.574703 | 8.4:-34550433 | MS.gene048333:CDS |
| TCTCAAAGCAATAACTTCGA+AGG | 0.575954 | 8.4:-34530010 | MS.gene048333:CDS |
| CCGGTTACCAGCATGTGACT+GGG | 0.576238 | 8.4:-34529739 | MS.gene048333:CDS |
| CCGTAACATTTGTTGCAATG+AGG | 0.578182 | 8.4:+34521253 | None:intergenic |
| TCGAGAAGGGAGTCGGCTGA+GGG | 0.581880 | 8.4:+34529780 | None:intergenic |
| AAAATGAGGACAAAACAGAA+TGG | 0.583227 | 8.4:-34549085 | MS.gene048333:CDS |
| CATTACTGGACCAGCTGACG+TGG | 0.584046 | 8.4:+34549169 | None:intergenic |
| GTGACTGTTCGTAATGTGCT+GGG | 0.585551 | 8.4:-34549964 | MS.gene048333:CDS |
| ATGGTTCCTTCAATTCCAGC+GGG | 0.587215 | 8.4:+34529911 | None:intergenic |
| TAACCCATAACGTTGAATTG+AGG | 0.588843 | 8.4:+34550311 | None:intergenic |
| TGAAGATGAGGAGGAAGACA+AGG | 0.591500 | 8.4:-34523217 | MS.gene048333:CDS |
| AGTGAAAACAAGCCCTAGCA+TGG | 0.591974 | 8.4:-34549324 | MS.gene048333:CDS |
| AAATGAGGAAGACGTAGTGA+TGG | 0.593757 | 8.4:-34530110 | MS.gene048333:CDS |
| CATGGTGATACTTATAGTGG+TGG | 0.595025 | 8.4:-34529614 | MS.gene048333:CDS |
| AGAATATGCAATCTTTGCTG+TGG | 0.597335 | 8.4:+34549130 | None:intergenic |
| GTTGTTGAGCCAATCTATCA+TGG | 0.597526 | 8.4:-34529632 | MS.gene048333:CDS |
| GAACAGTGTCAAATGTACTC+AGG | 0.598058 | 8.4:+34549231 | None:intergenic |
| AGCATGGCTGAAGCTGCTAT+GGG | 0.599364 | 8.4:-34549308 | MS.gene048333:CDS |
| TCAGCCGACTCCCTTCTCGA+CGG | 0.603425 | 8.4:-34529777 | MS.gene048333:CDS |
| GATAAGTCACATCGAACACC+TGG | 0.605867 | 8.4:+34523367 | None:intergenic |
| GTATTCGAACGGTGTTCGTG+AGG | 0.606073 | 8.4:+34550371 | None:intergenic |
| TAACATTTGTTGCAATGAGG+AGG | 0.606593 | 8.4:+34521256 | None:intergenic |
| CCTTCTCCAAGATCACCCCT+TGG | 0.609902 | 8.4:+34529656 | None:intergenic |
| GGAACAAAGGTCCTAGCAGA+AGG | 0.612302 | 8.4:-34549272 | MS.gene048333:CDS |
| AAAACCGTCGAGAAGGGAGT+CGG | 0.612389 | 8.4:+34529773 | None:intergenic |
| CCCATAACGTTGAATTGAGG+AGG | 0.613034 | 8.4:+34550314 | None:intergenic |
| ATCACCAGAAAAGCTCAAGA+AGG | 0.613647 | 8.4:-34539187 | MS.gene048333:intron |
| ATAACGTTGAATTGAGGAGG+AGG | 0.614589 | 8.4:+34550317 | None:intergenic |
| AAGAATTGCGCAGGGAACAA+AGG | 0.615276 | 8.4:-34549285 | MS.gene048333:CDS |
| ACAAAGGTCCTAGCAGAAGG+TGG | 0.616075 | 8.4:-34549269 | MS.gene048333:CDS |
| AAGAAGGCTGAGACTCTCGC+TGG | 0.621966 | 8.4:-34549910 | MS.gene048333:CDS |
| CATGTGACTGGGACTTCTAG+GGG | 0.623678 | 8.4:-34529728 | MS.gene048333:CDS |
| AATTGAAATACGTATTCGAA+CGG | 0.632788 | 8.4:+34550360 | None:intergenic |
| ATGTGCTGGGAAGATGGGGT+AGG | 0.636889 | 8.4:-34549951 | MS.gene048333:CDS |
| ATACTTATAGTGGTGGAGAA+GGG | 0.640736 | 8.4:-34529607 | MS.gene048333:CDS |
| GTCACATGCTGGTAACCGGT+CGG | 0.647933 | 8.4:+34529743 | None:intergenic |
| CTTGAACTATGATAACGCTG+TGG | 0.650671 | 8.4:-34544565 | MS.gene048333:CDS |
| CGTAATGTGCTGGGAAGATG+GGG | 0.660489 | 8.4:-34549955 | MS.gene048333:CDS |
| GATGGTTCCTTCAATTCCAG+CGG | 0.666037 | 8.4:+34529910 | None:intergenic |
| GAACACCTGGTATTCCTCTG+GGG | 0.673778 | 8.4:+34523380 | None:intergenic |
| AAGGGTCGTCAAAATCAAAG+AGG | 0.684157 | 8.4:-34529589 | MS.gene048333:CDS |
| ATGTGACTGGGACTTCTAGG+GGG | 0.700324 | 8.4:-34529727 | MS.gene048333:CDS |
| AACTATTATCAGAACCCCAG+AGG | 0.705621 | 8.4:-34523394 | MS.gene048333:CDS |
| GCTATGGGAAGAATTGCGCA+GGG | 0.710515 | 8.4:-34549293 | MS.gene048333:CDS |
| CTGATTGTGATCAGAGACGG+TGG | 0.720446 | 8.4:-34549986 | MS.gene048333:intron |
| GATGAGGCCCACTTTCCAAG+GGG | 0.725349 | 8.4:-34529671 | MS.gene048333:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 34521208 | 34550453 | 34521208 | ID=MS.gene048333 |
| chr8.4 | mRNA | 34521208 | 34550453 | 34521208 | ID=MS.gene048333.t1;Parent=MS.gene048333 |
| chr8.4 | exon | 34550315 | 34550453 | 34550315 | ID=MS.gene048333.t1.exon1;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34550315 | 34550453 | 34550315 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34549893 | 34549994 | 34549893 | ID=MS.gene048333.t1.exon2;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34549893 | 34549994 | 34549893 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34549073 | 34549344 | 34549073 | ID=MS.gene048333.t1.exon3;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34549073 | 34549344 | 34549073 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34544533 | 34544706 | 34544533 | ID=MS.gene048333.t1.exon4;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34544533 | 34544706 | 34544533 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34539188 | 34539273 | 34539188 | ID=MS.gene048333.t1.exon5;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34539188 | 34539273 | 34539188 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34529448 | 34530168 | 34529448 | ID=MS.gene048333.t1.exon6;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34529448 | 34530168 | 34529448 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34523209 | 34523448 | 34523209 | ID=MS.gene048333.t1.exon7;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34523209 | 34523448 | 34523209 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| chr8.4 | exon | 34521208 | 34521300 | 34521208 | ID=MS.gene048333.t1.exon8;Parent=MS.gene048333.t1 |
| chr8.4 | CDS | 34521208 | 34521300 | 34521208 | ID=cds.MS.gene048333.t1;Parent=MS.gene048333.t1 |
| Gene Sequence |
| Protein sequence |
