AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048435.t1 MTR_8g067280 96.302 649 23 1 1 649 1 648 0.0 1290
MS.gene048435.t1 MTR_5g030430 59.599 698 207 19 3 647 2 677 0.0 708
MS.gene048435.t1 MTR_5g030420 61.905 210 73 4 34 238 5 212 1.11e-84 266
MS.gene048435.t1 MTR_5g030420 61.905 210 73 4 34 238 5 212 1.88e-84 266
MS.gene048435.t1 MTR_8g024790 47.143 210 82 4 445 644 273 463 2.32e-52 187
MS.gene048435.t1 MTR_8g024790 30.137 219 115 6 43 257 19 203 3.17e-24 106
MS.gene048435.t1 MTR_8g024770 49.223 193 90 3 445 636 261 446 2.22e-50 182
MS.gene048435.t1 MTR_8g024770 27.624 181 88 5 67 243 36 177 7.73e-18 87.0
MS.gene048435.t1 MTR_8g027495 43.979 191 83 5 453 640 448 617 1.62e-36 145
MS.gene048435.t1 MTR_8g027495 34.694 196 110 8 52 234 51 241 2.71e-21 99.0
MS.gene048435.t1 MTR_7g096350 39.035 228 86 5 414 623 288 480 5.20e-36 142
MS.gene048435.t1 MTR_7g096350 29.348 184 122 6 53 234 49 226 1.24e-14 77.4
MS.gene048435.t1 MTR_7g117670 39.474 190 89 4 455 640 394 561 1.59e-33 135
MS.gene048435.t1 MTR_7g117670 29.439 214 114 9 52 239 48 250 5.67e-15 78.6
MS.gene048435.t1 MTR_4g067010 35.329 167 84 4 444 610 138 280 4.01e-20 92.4
MS.gene048435.t1 MTR_1g072320 31.579 190 109 6 54 234 11 188 1.03e-19 94.0
MS.gene048435.t1 MTR_1g072320 32.099 162 95 2 463 618 470 622 3.75e-19 92.0
MS.gene048435.t1 MTR_5g014640 36.970 165 71 5 452 609 147 285 1.52e-19 90.5
MS.gene048435.t1 MTR_0194s0050 29.592 294 163 14 385 641 97 383 2.41e-19 90.9
MS.gene048435.t1 MTR_0250s0040 36.943 157 86 3 463 618 88 232 4.20e-19 88.2
MS.gene048435.t1 MTR_2g104490 28.182 220 123 4 397 610 81 271 6.52e-19 88.6
MS.gene048435.t1 MTR_2g104490 29.412 221 119 6 397 610 81 271 6.91e-19 88.2
MS.gene048435.t1 MTR_0246s0020 31.401 207 119 5 449 645 190 383 6.92e-19 89.7
MS.gene048435.t1 MTR_4g009540 29.870 231 135 6 382 610 89 294 1.15e-18 87.8
MS.gene048435.t1 MTR_4g009540 29.870 231 135 6 382 610 89 294 2.15e-18 87.4
MS.gene048435.t1 MTR_4g009110 28.270 237 135 5 382 610 104 313 2.75e-18 87.4
MS.gene048435.t1 MTR_0246s0020 33.129 163 95 3 449 610 190 339 4.97e-18 86.7
MS.gene048435.t1 MTR_4g057270 33.333 162 93 6 467 625 153 302 6.93e-18 85.9
MS.gene048435.t1 MTR_0011s0260 36.000 150 81 4 463 610 183 319 9.23e-18 85.9
MS.gene048435.t1 MTR_0011s0210 35.333 150 82 4 463 610 183 319 1.27e-17 85.5
MS.gene048435.t1 MTR_2g010450 32.000 175 108 3 435 609 150 313 1.94e-17 84.7
MS.gene048435.t1 MTR_3g104100 32.000 150 101 1 467 616 199 347 5.21e-17 84.0
MS.gene048435.t1 MTR_4g066460 31.361 169 86 3 445 610 147 288 5.96e-17 82.8
MS.gene048435.t1 MTR_3g030700 32.530 166 106 2 451 616 184 343 7.43e-17 83.2
MS.gene048435.t1 MTR_4g092700 32.237 152 86 2 461 609 156 293 8.32e-17 82.4
MS.gene048435.t1 MTR_2g104550 27.442 215 134 5 397 610 82 275 1.76e-16 81.3
MS.gene048435.t1 MTR_6g047570 36.184 152 78 4 463 610 159 295 1.99e-16 81.6
MS.gene048435.t1 MTR_3g498695 35.256 156 96 3 461 614 296 448 3.42e-16 82.0
MS.gene048435.t1 MTR_3g498695 35.256 156 96 3 461 614 296 448 3.86e-16 82.0
MS.gene048435.t1 MTR_5g014520 31.515 165 100 3 446 610 142 293 6.24e-16 80.1
MS.gene048435.t1 MTR_4g066380 29.557 203 104 4 410 610 120 285 7.70e-16 79.7
MS.gene048435.t1 MTR_4g097950 32.768 177 78 4 439 586 25 189 8.96e-16 78.2
MS.gene048435.t1 MTR_2g038040 33.540 161 83 4 464 611 53 202 2.61e-15 76.6
MS.gene048435.t1 MTR_8g064700 33.544 158 92 5 468 619 249 399 4.80e-15 78.2
MS.gene048435.t1 MTR_2g084280 30.247 162 94 5 467 625 137 282 5.20e-15 77.0
MS.gene048435.t1 MTR_8g009020 30.058 173 101 4 447 610 125 286 6.77e-15 76.6
MS.gene048435.t1 MTR_5g014560 29.167 192 123 4 424 615 94 272 9.41e-15 75.9
MS.gene048435.t1 MTR_1g112680 31.325 166 89 6 467 611 321 482 2.23e-14 76.6
MS.gene048435.t1 MTR_8g098275 27.826 230 140 9 69 283 19 237 2.37e-14 76.6
MS.gene048435.t1 MTR_8g098275 28.834 163 101 3 459 606 397 559 1.13e-12 71.6
MS.gene048435.t1 MTR_2g081040 28.889 180 125 2 467 644 181 359 4.97e-14 74.3
MS.gene048435.t1 MTR_7g083900 33.333 162 101 3 461 618 284 442 1.02e-13 74.3
MS.gene048435.t1 MTR_7g083900 33.333 162 101 3 461 618 284 442 1.04e-13 74.3
MS.gene048435.t1 MTR_5g014600 45.833 72 39 0 445 516 131 202 1.87e-13 72.4
MS.gene048435.t1 MTR_4g097940 31.677 161 91 5 451 610 147 289 6.93e-13 70.5
MS.gene048435.t1 MTR_0246s0050 31.469 143 80 3 463 605 151 275 7.05e-13 70.5
MS.gene048435.t1 MTR_4g098035 31.469 143 80 3 463 605 172 296 7.82e-13 70.5
MS.gene048435.t1 MTR_6g047550 57.407 54 23 0 463 516 173 226 1.06e-12 69.3
MS.gene048435.t1 MTR_8g098275 28.834 163 101 3 459 606 304 466 1.29e-12 70.9
MS.gene048435.t1 MTR_8g098275 28.834 163 101 3 459 606 240 402 1.37e-12 70.9
MS.gene048435.t1 MTR_4g097920 30.337 178 94 6 440 610 115 269 1.45e-12 69.3
MS.gene048435.t1 MTR_4g097920 30.337 178 94 6 440 610 130 284 1.46e-12 69.7
MS.gene048435.t1 MTR_5g064950 26.147 218 137 8 436 635 70 281 4.84e-12 67.8
MS.gene048435.t1 MTR_2g104560 28.667 150 88 3 464 610 55 188 9.51e-12 65.9
MS.gene048435.t1 MTR_8g069740 27.160 162 103 5 452 610 102 251 1.71e-11 65.5
MS.gene048435.t1 MTR_8g066770 28.070 171 120 2 462 629 114 284 4.22e-11 65.1
MS.gene048435.t1 MTR_5g064950 25.510 196 124 7 436 615 70 259 4.72e-11 64.7
MS.gene048435.t1 MTR_1g028470 23.894 226 139 6 430 626 53 274 6.20e-11 64.3
MS.gene048435.t1 MTR_4g094762 29.240 171 114 3 464 630 293 460 7.42e-11 65.5
MS.gene048435.t1 MTR_2g022280 26.486 185 114 6 462 633 1 176 8.20e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048435.t1 AT1G32640 50.449 668 250 18 3 644 11 623 0.0 574
MS.gene048435.t1 AT4G17880 47.344 659 240 18 3 646 22 588 0.0 532
MS.gene048435.t1 AT5G46760 47.167 653 254 17 6 645 16 590 6.94e-169 495
MS.gene048435.t1 AT5G46830 57.068 191 67 4 454 643 334 510 1.40e-56 200
MS.gene048435.t1 AT5G46830 32.474 388 169 9 6 377 5 315 9.53e-43 162
MS.gene048435.t1 AT1G01260 38.202 267 112 6 374 640 362 575 3.75e-40 155
MS.gene048435.t1 AT1G01260 34.483 232 136 9 52 270 46 274 1.80e-25 111
MS.gene048435.t1 AT1G01260 38.202 267 112 6 374 640 362 575 3.75e-40 155
MS.gene048435.t1 AT1G01260 34.483 232 136 9 52 270 46 274 1.80e-25 111
MS.gene048435.t1 AT1G01260 38.202 267 112 6 374 640 362 575 3.75e-40 155
MS.gene048435.t1 AT1G01260 34.483 232 136 9 52 270 46 274 1.80e-25 111
MS.gene048435.t1 AT2G46510 42.051 195 82 3 445 637 377 542 2.57e-39 153
MS.gene048435.t1 AT2G46510 33.493 209 118 9 44 234 34 239 5.87e-21 97.4
MS.gene048435.t1 AT4G16430 40.341 176 69 4 445 619 302 442 3.42e-33 133
MS.gene048435.t1 AT4G16430 27.679 224 136 6 19 234 21 226 1.15e-16 83.6
MS.gene048435.t1 AT4G00870 46.000 150 73 2 462 611 248 389 8.79e-31 125
MS.gene048435.t1 AT4G00870 28.283 198 111 5 53 245 34 205 9.90e-17 83.6
MS.gene048435.t1 AT4G09820 34.343 198 114 7 58 245 20 211 6.64e-22 100
MS.gene048435.t1 AT4G09820 32.051 156 74 3 460 615 360 483 3.10e-16 82.4
MS.gene048435.t1 AT5G57150 31.844 179 116 3 464 640 56 230 1.28e-20 92.4
MS.gene048435.t1 AT5G57150 37.500 144 84 3 464 605 56 195 2.85e-20 90.5
MS.gene048435.t1 AT5G57150 37.500 144 84 3 464 605 55 194 2.90e-20 90.5
MS.gene048435.t1 AT5G57150 37.500 144 84 3 464 605 56 195 3.17e-20 90.1
MS.gene048435.t1 AT5G57150 37.500 144 84 3 464 605 55 194 6.08e-20 90.1
MS.gene048435.t1 AT4G37850 34.807 181 94 6 441 610 123 290 9.94e-20 91.3
MS.gene048435.t1 AT4G37850 34.807 181 94 6 441 610 123 290 9.94e-20 91.3
MS.gene048435.t1 AT1G63650 33.516 182 100 6 69 234 26 202 9.17e-18 87.8
MS.gene048435.t1 AT1G63650 24.551 167 119 1 459 618 401 567 1.47e-14 77.4
MS.gene048435.t1 AT1G63650 33.516 182 100 6 69 234 26 202 9.17e-18 87.8
MS.gene048435.t1 AT1G63650 24.551 167 119 1 459 618 401 567 1.47e-14 77.4
MS.gene048435.t1 AT1G63650 33.516 182 100 6 69 234 26 202 9.17e-18 87.8
MS.gene048435.t1 AT1G63650 24.551 167 119 1 459 618 401 567 1.47e-14 77.4
MS.gene048435.t1 AT3G26744 32.850 207 124 6 420 618 262 461 1.43e-17 86.7
MS.gene048435.t1 AT3G26744 32.850 207 124 6 420 618 262 461 1.68e-17 86.3
MS.gene048435.t1 AT3G26744 32.850 207 124 6 420 618 262 461 1.68e-17 86.3
MS.gene048435.t1 AT3G26744 32.850 207 124 6 420 618 262 461 1.68e-17 86.3
MS.gene048435.t1 AT2G28160 36.029 136 76 4 467 601 135 260 5.76e-17 82.8
MS.gene048435.t1 AT2G28160 36.029 136 76 4 467 601 135 260 6.10e-17 82.8
MS.gene048435.t1 AT3G24140 29.048 210 126 6 445 633 177 384 6.38e-17 84.0
MS.gene048435.t1 AT3G24140 29.048 210 126 6 445 633 177 384 7.58e-17 83.6
MS.gene048435.t1 AT4G29930 31.875 160 83 4 447 605 43 177 1.85e-16 79.7
MS.gene048435.t1 AT1G12860 33.929 168 86 4 461 618 265 417 3.43e-16 81.6
MS.gene048435.t1 AT4G29930 31.875 160 83 4 447 605 43 177 3.57e-16 78.6
MS.gene048435.t1 AT1G12860 33.929 168 86 4 461 618 265 417 5.08e-16 81.6
MS.gene048435.t1 AT4G29930 31.677 161 85 4 447 605 43 180 5.62e-16 78.2
MS.gene048435.t1 AT2G22750 30.047 213 130 4 407 610 93 295 1.04e-15 79.3
MS.gene048435.t1 AT2G22750 30.047 213 130 4 407 610 93 295 1.24e-15 79.0
MS.gene048435.t1 AT2G22750 30.047 213 130 4 407 610 89 291 1.28e-15 79.0
MS.gene048435.t1 AT2G22750 30.047 213 130 4 407 610 65 267 1.63e-15 78.2
MS.gene048435.t1 AT2G22750 30.047 213 130 4 407 610 65 267 1.93e-15 78.2
MS.gene048435.t1 AT4G29930 33.987 153 89 4 464 605 55 206 2.54e-15 77.0
MS.gene048435.t1 AT4G29930 33.987 153 89 4 464 605 55 206 2.54e-15 77.0
MS.gene048435.t1 AT4G29930 33.987 153 89 4 464 605 55 206 4.49e-15 76.3
MS.gene048435.t1 AT4G00480 29.348 184 104 6 69 231 36 214 3.64e-14 75.9
MS.gene048435.t1 AT5G41315 27.429 175 113 4 457 618 435 608 4.16e-14 76.3
MS.gene048435.t1 AT5G41315 27.957 186 108 5 69 234 27 206 6.65e-14 75.5
MS.gene048435.t1 AT4G00480 27.523 218 130 7 35 231 4 214 4.26e-14 75.9
MS.gene048435.t1 AT5G41315 27.429 175 113 4 457 618 443 616 4.47e-14 76.3
MS.gene048435.t1 AT5G41315 27.957 186 108 5 69 234 35 214 6.84e-14 75.5
MS.gene048435.t1 AT5G41315 27.429 175 113 4 457 618 440 613 4.61e-14 75.9
MS.gene048435.t1 AT5G41315 27.957 186 108 5 69 234 32 211 6.70e-14 75.5
MS.gene048435.t1 AT4G00480 29.348 184 104 6 69 231 36 214 5.65e-14 75.5
MS.gene048435.t1 AT4G29930 54.237 59 27 0 465 523 56 114 1.36e-13 70.1
MS.gene048435.t1 AT2G22760 32.738 168 83 5 452 610 112 258 6.86e-13 70.1
MS.gene048435.t1 AT2G16910 30.189 159 94 4 467 611 318 473 9.75e-13 71.6
MS.gene048435.t1 AT2G22760 32.738 168 83 5 452 610 112 258 1.06e-12 69.7
MS.gene048435.t1 AT1G10610 34.028 144 84 5 464 605 168 302 1.78e-12 69.7
MS.gene048435.t1 AT5G10570 29.231 195 111 5 436 630 126 293 1.94e-12 69.3
MS.gene048435.t1 AT1G10610 34.028 144 84 5 464 605 235 369 2.40e-12 69.7
MS.gene048435.t1 AT1G10610 34.028 144 84 5 464 605 265 399 2.58e-12 69.7
MS.gene048435.t1 AT2G22770 28.191 188 113 4 457 629 123 303 5.90e-12 67.8

Find 164 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCGTAGAGCGTAGAATTTC+TGG 0.101182 8.3:+35801183 None:intergenic
CAGAGATGCTTTGTCCATTT+TGG 0.115615 8.3:+35801142 None:intergenic
TCCTTGTGCATCACCAATTT+TGG 0.145524 8.3:+35800689 None:intergenic
TCTCTTCTTTCTTCCAAAAT+TGG 0.173669 8.3:-35800702 MS.gene048435:CDS
CTTCGATGAGCTTGTTCTTC+TGG 0.206409 8.3:+35802283 None:intergenic
GAGCTTAATACGTTGATTTC+AGG 0.216229 8.3:-35802247 MS.gene048435:CDS
CCTCTGACCTCTCTAATTTC+TGG 0.240254 8.3:-35802558 MS.gene048435:CDS
GGATATTGAAGTGAAGATTA+TGG 0.243770 8.3:-35800910 MS.gene048435:CDS
CTCAGAATCTCCCCGGATTC+TGG 0.249063 8.3:+35801578 None:intergenic
TGGCTACACCGCTGCTCGGC+TGG 0.254416 8.3:-35802363 MS.gene048435:CDS
GGTTCCAAATGTTTCCAAAA+TGG 0.283532 8.3:-35801156 MS.gene048435:CDS
TCCATCGTCAATGCTGCTTC+TGG 0.290947 8.3:+35801445 None:intergenic
CAAACAATATGAATAACATT+TGG 0.318173 8.3:-35802618 None:intergenic
ACCGGCCACGAACCTGTTTC+AGG 0.325408 8.3:+35801896 None:intergenic
GGCTACACCGCTGCTCGGCT+GGG 0.333611 8.3:-35802362 MS.gene048435:CDS
TGCAGAAATGGTTGCGTTCA+TGG 0.335709 8.3:+35801760 None:intergenic
TCCATACAACGCTGATCTAA+TGG 0.343232 8.3:-35801951 MS.gene048435:CDS
CCGCTGCTCGGCTGGGGTGA+TGG 0.351635 8.3:-35802355 MS.gene048435:CDS
GGTGAATTGGAGAAACAATT+AGG 0.352788 8.3:-35801053 MS.gene048435:CDS
CTCTTCTAAGTTAATTGACT+TGG 0.357805 8.3:-35800931 MS.gene048435:CDS
AGTTCTTATATCACCGGAAA+TGG 0.362072 8.3:-35801536 MS.gene048435:CDS
GGTCAGTCACAGTTCGTTGC+CGG 0.380141 8.3:-35801500 MS.gene048435:CDS
GCCCGTGAAGCACGGGTCCA+TGG 0.380533 8.3:-35802043 MS.gene048435:CDS
CGTTTCTTCGGTCGCTTCCC+TGG 0.384757 8.3:+35801284 None:intergenic
TCCATTAGATCAGCGTTGTA+TGG 0.386007 8.3:+35801950 None:intergenic
AGTAGCAGGGTGGTTGAGCC+AGG 0.388458 8.3:-35801302 MS.gene048435:CDS
AATTTCAACAATCCTGAAAC+AGG 0.392528 8.3:-35801908 MS.gene048435:CDS
TCAACCCCGGTGTGGATCAC+CGG 0.394753 8.3:-35802100 MS.gene048435:CDS
GGAATGCTTTCGTTTACTTC+CGG 0.395195 8.3:-35801425 MS.gene048435:CDS
CCCATGGACCCGTGCTTCAC+GGG 0.396142 8.3:+35802042 None:intergenic
AGAAAAGAAAGTTCCATTTC+CGG 0.399215 8.3:+35801523 None:intergenic
TGAAGGAAGGAGAGAGTAGC+AGG 0.399337 8.3:-35801316 MS.gene048435:CDS
CGTCTTCAAGCTCTGATTGA+AGG 0.400185 8.3:-35802442 MS.gene048435:CDS
AAACAATATGAATAACATTT+GGG 0.401501 8.3:-35802617 None:intergenic
ATCGTTCTCGCTCGTTGTAA+TGG 0.403129 8.3:+35801868 None:intergenic
TGGGTTGGGATGCTATGATT+AGG 0.406285 8.3:-35800890 MS.gene048435:CDS
GATATTGAAGTGAAGATTAT+GGG 0.406522 8.3:-35800909 MS.gene048435:CDS
TGGTGTTTCCGTTAGAGTAC+TGG 0.408218 8.3:+35801691 None:intergenic
TACTAATTCTGTCTGTGAAC+GGG 0.410519 8.3:-35802065 MS.gene048435:CDS
AGGTACGGTGGCAGTGTTAA+CGG 0.412615 8.3:+35801784 None:intergenic
TCCGGGGAGATTCTGAGTTT+CGG 0.416724 8.3:-35801572 MS.gene048435:CDS
GGAATCGTCAAAAGGTGAAT+TGG 0.427183 8.3:-35801066 MS.gene048435:CDS
TCATTACCGTTTGAAACAAA+TGG 0.429184 8.3:-35801719 MS.gene048435:CDS
CAGCCCCGGTGATCCACACC+GGG 0.439785 8.3:+35802095 None:intergenic
GTTGAACCATTTGTTTCAAA+CGG 0.440221 8.3:+35801713 None:intergenic
ACCCATGGACCCGTGCTTCA+CGG 0.442980 8.3:+35802041 None:intergenic
CAACCCCGGTGTGGATCACC+GGG 0.445985 8.3:-35802099 MS.gene048435:CDS
GATTGTTGCATTTGCAGAAA+TGG 0.449234 8.3:+35801748 None:intergenic
CGGCACCGGGTCACTGAGCC+AGG 0.450416 8.3:-35802134 MS.gene048435:CDS
CGAGGTAGAAAACCGGCTAA+TGG 0.451911 8.3:-35801263 MS.gene048435:CDS
TCAATGCTGCTTCTGGATAC+AGG 0.460912 8.3:+35801452 None:intergenic
GGTTCAATGAAACCAGAATC+CGG 0.461046 8.3:-35801590 MS.gene048435:CDS
AGAACGATCCTTCATCTGTC+TGG 0.464418 8.3:-35801853 MS.gene048435:CDS
TCATCCTGCGGCGAAGTTGA+TGG 0.465184 8.3:-35800847 MS.gene048435:CDS
CAATCCTTTGTCAACGGCAC+CGG 0.467486 8.3:-35802148 MS.gene048435:CDS
CCCGTGAAGCACGGGTCCAT+GGG 0.472068 8.3:-35802042 MS.gene048435:CDS
TGAAGTGAAGATTATGGGTT+GGG 0.485063 8.3:-35800904 MS.gene048435:CDS
TGTGGATCACCGGGGCTGAA+AGG 0.486911 8.3:-35802090 MS.gene048435:CDS
TCTCTCTGCCTCTGCAGCTA+TGG 0.487618 8.3:-35801822 MS.gene048435:CDS
TCAGCCCCGGTGATCCACAC+CGG 0.489282 8.3:+35802094 None:intergenic
GGTTCTTCTCTTCCATTAGC+CGG 0.494454 8.3:+35801251 None:intergenic
GTGAACGGGCCCGTGAAGCA+CGG 0.499419 8.3:-35802051 MS.gene048435:CDS
GCTACACCGCTGCTCGGCTG+GGG 0.501207 8.3:-35802361 MS.gene048435:CDS
TCAAGCAGCCGAGGTGGAGG+CGG 0.502710 8.3:-35801374 MS.gene048435:CDS
TTGAAGGCGCGAAAGAAAGC+TGG 0.503041 8.3:-35802426 MS.gene048435:CDS
GCCGCCATCAACTTCGCCGC+AGG 0.505374 8.3:+35800843 None:intergenic
ATTAGTAAGCCTTTCAGCCC+CGG 0.505431 8.3:+35802081 None:intergenic
CGATGAGCTTGTTCTTCTGG+AGG 0.507325 8.3:+35802286 None:intergenic
GGTCCATGGGTTTCAGACCT+TGG 0.507823 8.3:-35802029 MS.gene048435:CDS
GAAATCGAAGCTACAAGGGC+TGG 0.510964 8.3:-35801087 MS.gene048435:CDS
TCAACAAGCTTCTGTTAACA+TGG 0.511118 8.3:-35800757 MS.gene048435:CDS
TGTTGTGATGGAGGAGGCAC+TGG 0.512388 8.3:+35802496 None:intergenic
GCCGAGCAGCGGTGTAGCCA+TGG 0.518239 8.3:+35802366 None:intergenic
AAGAAAAGTTCTTATATCAC+CGG 0.523073 8.3:-35801542 MS.gene048435:CDS
TTGAAGTGAAGATTATGGGT+TGG 0.525361 8.3:-35800905 MS.gene048435:CDS
GAAATGGTTGCGTTCATGGC+AGG 0.525493 8.3:+35801764 None:intergenic
TGAACGGGCCCGTGAAGCAC+GGG 0.527585 8.3:-35802050 MS.gene048435:CDS
TCAGAGCTTGAAGACGGTGT+TGG 0.533151 8.3:+35802449 None:intergenic
AAATCAAGCAGCCGAGGTGG+AGG 0.534413 8.3:-35801377 MS.gene048435:CDS
TCCAAAATTGGTGATGCACA+AGG 0.537190 8.3:-35800690 MS.gene048435:CDS
CAAGGGCTGGAATCGTCAAA+AGG 0.538390 8.3:-35801074 MS.gene048435:CDS
ACAATTAGGTGCAACAAAGA+AGG 0.539042 8.3:-35801039 MS.gene048435:CDS
TCATTGAGCCAGACAGATGA+AGG 0.541127 8.3:+35801845 None:intergenic
GTTCAATGAAACCAGAATCC+GGG 0.541459 8.3:-35801589 MS.gene048435:CDS
CTTGATTTCATATTTGAAGA+CGG 0.542319 8.3:+35801392 None:intergenic
AATTCCATTAGATAAAGAAA+AGG 0.545738 8.3:-35800976 MS.gene048435:CDS
TCTAGATGTTTCTGCTGTGA+AGG 0.546467 8.3:-35801333 MS.gene048435:CDS
ATTTCTGCAAATGCAACAAT+CGG 0.548088 8.3:-35801746 MS.gene048435:CDS
GGGGTGATGGATATTACAAA+GGG 0.549464 8.3:-35802342 MS.gene048435:CDS
CTTAATACGTTGATTTCAGG+TGG 0.552157 8.3:-35802244 MS.gene048435:CDS
GAATTGAAATCGAAGCTACA+AGG 0.554225 8.3:-35801092 MS.gene048435:CDS
TCCTGAAACAGGTTCGTGGC+CGG 0.556011 8.3:-35801897 MS.gene048435:CDS
ACCGAAACTCAGAATCTCCC+CGG 0.557140 8.3:+35801571 None:intergenic
AATCCTTTGTCAACGGCACC+GGG 0.557176 8.3:-35802147 MS.gene048435:CDS
ACCATGGCTACACCGCTGCT+CGG 0.557176 8.3:-35802367 MS.gene048435:CDS
AGCCATGGTGTAATCATAAG+AGG 0.557540 8.3:+35802381 None:intergenic
GGTTGCGTTCATGGCAGGTA+CGG 0.559424 8.3:+35801769 None:intergenic
TTACTAATTCTGTCTGTGAA+CGG 0.561586 8.3:-35802066 MS.gene048435:CDS
ATAAGCCTGGCTCAGTGACC+CGG 0.565475 8.3:+35802129 None:intergenic
GATGTTTCTGCTGTGAAGGA+AGG 0.568459 8.3:-35801329 MS.gene048435:CDS
TGAGCAAAGTTAACTGCAGT+TGG 0.569736 8.3:+35801671 None:intergenic
GAAGTTGCGGAATGTGGTGT+TGG 0.570968 8.3:+35802532 None:intergenic
TGCGTTCATGGCAGGTACGG+TGG 0.575421 8.3:+35801772 None:intergenic
GAAGAAACGAGGTAGAAAAC+CGG 0.575492 8.3:-35801270 MS.gene048435:CDS
GTTGTTGAAGTTGCGGAATG+TGG 0.580780 8.3:+35802526 None:intergenic
ATGGACAAAGCATCTCTGCT+AGG 0.582052 8.3:-35801137 MS.gene048435:CDS
TCGAATTTCCATAGCTGCAG+AGG 0.584390 8.3:+35801814 None:intergenic
GGACGACAACTCCTCCGTCA+TGG 0.585254 8.3:-35802596 MS.gene048435:CDS
ACAATCCTGAAACAGGTTCG+TGG 0.586854 8.3:-35801901 MS.gene048435:CDS
CTCGTTGTAATGGAATTCAC+CGG 0.588226 8.3:+35801878 None:intergenic
GGGTTGAATTGAAATAAGCC+TGG 0.588534 8.3:+35802116 None:intergenic
CCATCACCCCAGCCGAGCAG+CGG 0.593117 8.3:+35802355 None:intergenic
GGCTTATTTCAATTCAACCC+CGG 0.593927 8.3:-35802113 MS.gene048435:CDS
GTATACCAACGTCTTCAAGC+GGG 0.595425 8.3:-35802003 MS.gene048435:CDS
TGACCCGGTGCCGTTGACAA+AGG 0.598401 8.3:+35802144 None:intergenic
GTCGGTGACATCTTCCTCAA+CGG 0.604811 8.3:+35802201 None:intergenic
TGTCTCAGATGATGCCGTTG+AGG 0.605605 8.3:-35802215 MS.gene048435:CDS
ATTCTACGCTCTACGCGCTG+TGG 0.607114 8.3:-35801177 MS.gene048435:CDS
ATGAAATCAAGCAGCCGAGG+TGG 0.610118 8.3:-35801380 MS.gene048435:CDS
GGCGTTGAAAGACTTAGACT+TGG 0.610935 8.3:-35800823 MS.gene048435:CDS
CAACAAGCTTCTGTTAACAT+GGG 0.613453 8.3:-35800756 MS.gene048435:CDS
GTAGCAGGGTGGTTGAGCCA+GGG 0.614057 8.3:-35801301 MS.gene048435:CDS
TGGTGCAGTTGTTGAAGTTG+CGG 0.614269 8.3:+35802519 None:intergenic
TGGTTCAACCAGTACTCTAA+CGG 0.614412 8.3:-35801699 MS.gene048435:CDS
AAATCAACGTATTAAGCTCA+CGG 0.614664 8.3:+35802251 None:intergenic
CTTCAATCAGAGCTTGAAGA+CGG 0.614935 8.3:+35802443 None:intergenic
TCTGCTTCTACATGATTCAA+CGG 0.616515 8.3:+35801230 None:intergenic
TGTGATGGAGGAGGCACTGG+TGG 0.617060 8.3:+35802499 None:intergenic
CAGAATCTCCGCCTCCACCT+CGG 0.617972 8.3:+35801366 None:intergenic
ATTTCAATTCAACCCCGGTG+TGG 0.618802 8.3:-35802108 MS.gene048435:CDS
AACATAGACTGTTGTGATGG+AGG 0.624047 8.3:+35802487 None:intergenic
TTGAACATAGACTGTTGTGA+TGG 0.627469 8.3:+35802484 None:intergenic
ATGACTCAATCCTTTGTCAA+CGG 0.630282 8.3:-35802154 MS.gene048435:CDS
GGAAGGAGAGAGTAGCAGGG+TGG 0.637372 8.3:-35801312 MS.gene048435:CDS
TCCTGCGGCGAAGTTGATGG+CGG 0.639474 8.3:-35800844 MS.gene048435:CDS
TCCAGAAGCAGCATTGACGA+TGG 0.640563 8.3:-35801446 MS.gene048435:CDS
TGGGGTGATGGATATTACAA+AGG 0.644784 8.3:-35802343 MS.gene048435:CDS
TGTATACCAACGTCTTCAAG+CGG 0.647168 8.3:-35802004 MS.gene048435:CDS
ATACCAAGGTCTGAAACCCA+TGG 0.647395 8.3:+35802026 None:intergenic
CCAGAAATTAGAGAGGTCAG+AGG 0.648435 8.3:+35802558 None:intergenic
TTCAATGAAACCAGAATCCG+GGG 0.651102 8.3:-35801588 MS.gene048435:CDS
ACCACCCCGCTTGAAGACGT+TGG 0.653527 8.3:+35801998 None:intergenic
GGGAAGCGACCGAAGAAACG+AGG 0.656232 8.3:-35801281 MS.gene048435:CDS
GAAATTAGAGAGGTCAGAGG+TGG 0.659018 8.3:+35802561 None:intergenic
ATAGACTGTTGTGATGGAGG+AGG 0.660918 8.3:+35802490 None:intergenic
GGTCATGAAAGCTTCCATGA+CGG 0.664445 8.3:+35802582 None:intergenic
TATACCAACGTCTTCAAGCG+GGG 0.672915 8.3:-35802002 MS.gene048435:CDS
TACAAAGGGGAAGATGACAA+AGG 0.675098 8.3:-35802328 MS.gene048435:CDS
AAGATGTCACCGACACTGAG+TGG 0.679436 8.3:-35802192 MS.gene048435:CDS
CATGAAAGCTTCCATGACGG+AGG 0.681601 8.3:+35802585 None:intergenic
GAAGGAAGGAGAGAGTAGCA+GGG 0.683186 8.3:-35801315 MS.gene048435:CDS
AATTGAAATCGAAGCTACAA+GGG 0.684780 8.3:-35801091 MS.gene048435:CDS
AGACGTTGGTATACATACCA+AGG 0.685367 8.3:+35802012 None:intergenic
AATATGAAATCAAGCAGCCG+AGG 0.686490 8.3:-35801383 MS.gene048435:CDS
GGATTGCCAGAAAATCGCGT+AGG 0.687237 8.3:+35802402 None:intergenic
TGAAGACGGTAAGACTACAC+CGG 0.689623 8.3:+35801406 None:intergenic
ATCCTCTTATGATTACACCA+TGG 0.705200 8.3:-35802383 MS.gene048435:CDS
ACCAACGTCTTCAAGCGGGG+TGG 0.708219 8.3:-35801999 MS.gene048435:CDS
TTGGAAGCCAGAAATTAGAG+AGG 0.708961 8.3:+35802551 None:intergenic
CAGCAAGAAGAATCATCCTG+CGG 0.713584 8.3:-35800859 MS.gene048435:CDS
AGCCCCGGTGATCCACACCG+GGG 0.732020 8.3:+35802096 None:intergenic
TACGGTGGCAGTGTTAACGG+TGG 0.759784 8.3:+35801787 None:intergenic
AACCCCGGTGTGGATCACCG+GGG 0.772910 8.3:-35802098 MS.gene048435:CDS
GGGTGATGGATATTACAAAG+GGG 0.787460 8.3:-35802341 MS.gene048435:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTCCATTAGATAAAGAAA+AGG - chr8.3:35802317-35802336 MS.gene048435:CDS 20.0%
!!! TTTTCTTTATCTAATGGAAT+TGG + chr8.3:35802318-35802337 None:intergenic 20.0%
! AAGAAAAGTTCTTATATCAC+CGG - chr8.3:35801751-35801770 MS.gene048435:CDS 25.0%
! CTTGATTTCATATTTGAAGA+CGG + chr8.3:35801904-35801923 None:intergenic 25.0%
! GATATTGAAGTGAAGATTAT+GGG - chr8.3:35802384-35802403 MS.gene048435:CDS 25.0%
!! TTCTCCTTTTCTTTATCTAA+TGG + chr8.3:35802324-35802343 None:intergenic 25.0%
AAATCAACGTATTAAGCTCA+CGG + chr8.3:35801045-35801064 None:intergenic 30.0%
AATTGAAATCGAAGCTACAA+GGG - chr8.3:35802202-35802221 MS.gene048435:CDS 30.0%
AATTTCAACAATCCTGAAAC+AGG - chr8.3:35801385-35801404 MS.gene048435:CDS 30.0%
AGAAAAGAAAGTTCCATTTC+CGG + chr8.3:35801773-35801792 None:intergenic 30.0%
ATTTCTGCAAATGCAACAAT+CGG - chr8.3:35801547-35801566 MS.gene048435:CDS 30.0%
CTCTTCTAAGTTAATTGACT+TGG - chr8.3:35802362-35802381 MS.gene048435:CDS 30.0%
GGATATTGAAGTGAAGATTA+TGG - chr8.3:35802383-35802402 MS.gene048435:CDS 30.0%
GTTGAACCATTTGTTTCAAA+CGG + chr8.3:35801583-35801602 None:intergenic 30.0%
TCTCTTCTTTCTTCCAAAAT+TGG - chr8.3:35802591-35802610 MS.gene048435:CDS 30.0%
TTACTAATTCTGTCTGTGAA+CGG - chr8.3:35801227-35801246 MS.gene048435:CDS 30.0%
! TCATTACCGTTTGAAACAAA+TGG - chr8.3:35801574-35801593 MS.gene048435:CDS 30.0%
! TCTTTTTCTGTTTTCTTCAC+CGG + chr8.3:35801815-35801834 None:intergenic 30.0%
!! TTGTCCATTTTGGAAACATT+TGG + chr8.3:35802144-35802163 None:intergenic 30.0%
ACAATTAGGTGCAACAAAGA+AGG - chr8.3:35802254-35802273 MS.gene048435:CDS 35.0%
AGTTCTTATATCACCGGAAA+TGG - chr8.3:35801757-35801776 MS.gene048435:CDS 35.0%
ATCCTCTTATGATTACACCA+TGG - chr8.3:35800910-35800929 MS.gene048435:CDS 35.0%
ATGACTCAATCCTTTGTCAA+CGG - chr8.3:35801139-35801158 MS.gene048435:CDS 35.0%
CAACAAGCTTCTGTTAACAT+GGG - chr8.3:35802537-35802556 MS.gene048435:CDS 35.0%
GAATTGAAATCGAAGCTACA+AGG - chr8.3:35802201-35802220 MS.gene048435:CDS 35.0%
GATTGTTGCATTTGCAGAAA+TGG + chr8.3:35801548-35801567 None:intergenic 35.0%
GGTGAATTGGAGAAACAATT+AGG - chr8.3:35802240-35802259 MS.gene048435:CDS 35.0%
GGTTCCAAATGTTTCCAAAA+TGG - chr8.3:35802137-35802156 MS.gene048435:CDS 35.0%
TACTAATTCTGTCTGTGAAC+GGG - chr8.3:35801228-35801247 MS.gene048435:CDS 35.0%
TCAACAAGCTTCTGTTAACA+TGG - chr8.3:35802536-35802555 MS.gene048435:CDS 35.0%
TCTGCTTCTACATGATTCAA+CGG + chr8.3:35802066-35802085 None:intergenic 35.0%
TGAAGTGAAGATTATGGGTT+GGG - chr8.3:35802389-35802408 MS.gene048435:CDS 35.0%
TTGAACATAGACTGTTGTGA+TGG + chr8.3:35800812-35800831 None:intergenic 35.0%
TTGAAGTGAAGATTATGGGT+TGG - chr8.3:35802388-35802407 MS.gene048435:CDS 35.0%
! CTGAAGGAACTGAATTTTTC+TGG - chr8.3:35801682-35801701 MS.gene048435:CDS 35.0%
! GGAAATGGAACTTTCTTTTC+TGG - chr8.3:35801772-35801791 MS.gene048435:CDS 35.0%
!! CTTAATACGTTGATTTCAGG+TGG - chr8.3:35801049-35801068 MS.gene048435:CDS 35.0%
!! GAGCTTAATACGTTGATTTC+AGG - chr8.3:35801046-35801065 MS.gene048435:CDS 35.0%
AACATAGACTGTTGTGATGG+AGG + chr8.3:35800809-35800828 None:intergenic 40.0%
AATATGAAATCAAGCAGCCG+AGG - chr8.3:35801910-35801929 MS.gene048435:CDS 40.0%
AGACGTTGGTATACATACCA+AGG + chr8.3:35801284-35801303 None:intergenic 40.0%
AGCCATGGTGTAATCATAAG+AGG + chr8.3:35800915-35800934 None:intergenic 40.0%
CTTCAATCAGAGCTTGAAGA+CGG + chr8.3:35800853-35800872 None:intergenic 40.0%
GAAGAAACGAGGTAGAAAAC+CGG - chr8.3:35802023-35802042 MS.gene048435:CDS 40.0%
GAGAAAAAACCACTCAGTGT+CGG + chr8.3:35801113-35801132 None:intergenic 40.0%
GGAATCGTCAAAAGGTGAAT+TGG - chr8.3:35802227-35802246 MS.gene048435:CDS 40.0%
GGAATGCTTTCGTTTACTTC+CGG - chr8.3:35801868-35801887 MS.gene048435:CDS 40.0%
GGCTTATTTCAATTCAACCC+CGG - chr8.3:35801180-35801199 MS.gene048435:CDS 40.0%
GGGTTGAATTGAAATAAGCC+TGG + chr8.3:35801180-35801199 None:intergenic 40.0%
GGTTCAATGAAACCAGAATC+CGG - chr8.3:35801703-35801722 MS.gene048435:CDS 40.0%
GTTCAATGAAACCAGAATCC+GGG - chr8.3:35801704-35801723 MS.gene048435:CDS 40.0%
GTTCCTTCAGAAAAAAGCTC+TGG + chr8.3:35801672-35801691 None:intergenic 40.0%
TACAAAGGGGAAGATGACAA+AGG - chr8.3:35800965-35800984 MS.gene048435:CDS 40.0%
TCCATACAACGCTGATCTAA+TGG - chr8.3:35801342-35801361 MS.gene048435:CDS 40.0%
TCCATTAGATCAGCGTTGTA+TGG + chr8.3:35801346-35801365 None:intergenic 40.0%
TGAGCAAAGTTAACTGCAGT+TGG + chr8.3:35801625-35801644 None:intergenic 40.0%
TGGTTCAACCAGTACTCTAA+CGG - chr8.3:35801594-35801613 MS.gene048435:CDS 40.0%
TGTATACCAACGTCTTCAAG+CGG - chr8.3:35801289-35801308 MS.gene048435:CDS 40.0%
TTCAATGAAACCAGAATCCG+GGG - chr8.3:35801705-35801724 MS.gene048435:CDS 40.0%
TTGGAAGCCAGAAATTAGAG+AGG + chr8.3:35800745-35800764 None:intergenic 40.0%
! CAGAGATGCTTTGTCCATTT+TGG + chr8.3:35802154-35802173 None:intergenic 40.0%
! CTCGTTGTAATGGAATTCAC+CGG + chr8.3:35801418-35801437 None:intergenic 40.0%
! GGTTTTCTACCTCGTTTCTT+CGG + chr8.3:35802024-35802043 None:intergenic 40.0%
! TCAGAAAAAAGCTCTGGTTC+TGG + chr8.3:35801666-35801685 None:intergenic 40.0%
! TCCTTGTGCATCACCAATTT+TGG + chr8.3:35802607-35802626 None:intergenic 40.0%
! TCTAGATGTTTCTGCTGTGA+AGG - chr8.3:35801960-35801979 MS.gene048435:CDS 40.0%
!! GGGGTGATGGATATTACAAA+GGG - chr8.3:35800951-35800970 MS.gene048435:CDS 40.0%
!! GGGTGATGGATATTACAAAG+GGG - chr8.3:35800952-35800971 MS.gene048435:CDS 40.0%
!! TCCAAAATTGGTGATGCACA+AGG - chr8.3:35802603-35802622 MS.gene048435:CDS 40.0%
!! TGGGGTGATGGATATTACAA+AGG - chr8.3:35800950-35800969 MS.gene048435:CDS 40.0%
!!! GAACCAGAGCTTTTTTCTGA+AGG - chr8.3:35801666-35801685 MS.gene048435:CDS 40.0%
ACAATCCTGAAACAGGTTCG+TGG - chr8.3:35801392-35801411 MS.gene048435:CDS 45.0%
AGAACGATCCTTCATCTGTC+TGG - chr8.3:35801440-35801459 MS.gene048435:CDS 45.0%
ATCGTTCTCGCTCGTTGTAA+TGG + chr8.3:35801428-35801447 None:intergenic 45.0%
ATTAGTAAGCCTTTCAGCCC+CGG + chr8.3:35801215-35801234 None:intergenic 45.0%
ATTTCAATTCAACCCCGGTG+TGG - chr8.3:35801185-35801204 MS.gene048435:CDS 45.0%
CAGCAAGAAGAATCATCCTG+CGG - chr8.3:35802434-35802453 MS.gene048435:CDS 45.0%
CCAGAAATTAGAGAGGTCAG+AGG + chr8.3:35800738-35800757 None:intergenic 45.0%
CCTCTGACCTCTCTAATTTC+TGG - chr8.3:35800735-35800754 MS.gene048435:CDS 45.0%
GAAATTAGAGAGGTCAGAGG+TGG + chr8.3:35800735-35800754 None:intergenic 45.0%
GGTCATGAAAGCTTCCATGA+CGG + chr8.3:35800714-35800733 None:intergenic 45.0%
GGTTCTTCTCTTCCATTAGC+CGG + chr8.3:35802045-35802064 None:intergenic 45.0%
GTATACCAACGTCTTCAAGC+GGG - chr8.3:35801290-35801309 MS.gene048435:CDS 45.0%
GTTGTTGAAGTTGCGGAATG+TGG + chr8.3:35800770-35800789 None:intergenic 45.0%
TATACCAACGTCTTCAAGCG+GGG - chr8.3:35801291-35801310 MS.gene048435:CDS 45.0%
TCATTGAGCCAGACAGATGA+AGG + chr8.3:35801451-35801470 None:intergenic 45.0%
TCGAATTTCCATAGCTGCAG+AGG + chr8.3:35801482-35801501 None:intergenic 45.0%
TGAAGACGGTAAGACTACAC+CGG + chr8.3:35801890-35801909 None:intergenic 45.0%
TGCAGAAATGGTTGCGTTCA+TGG + chr8.3:35801536-35801555 None:intergenic 45.0%
TGGTGCAGTTGTTGAAGTTG+CGG + chr8.3:35800777-35800796 None:intergenic 45.0%
TGGTGTTTCCGTTAGAGTAC+TGG + chr8.3:35801605-35801624 None:intergenic 45.0%
! ATACCAAGGTCTGAAACCCA+TGG + chr8.3:35801270-35801289 None:intergenic 45.0%
! ATAGACTGTTGTGATGGAGG+AGG + chr8.3:35800806-35800825 None:intergenic 45.0%
! ATGGACAAAGCATCTCTGCT+AGG - chr8.3:35802156-35802175 MS.gene048435:CDS 45.0%
! CGTCTTCAAGCTCTGATTGA+AGG - chr8.3:35800851-35800870 MS.gene048435:CDS 45.0%
! CTTCGATGAGCTTGTTCTTC+TGG + chr8.3:35801013-35801032 None:intergenic 45.0%
! GATGTTTCTGCTGTGAAGGA+AGG - chr8.3:35801964-35801983 MS.gene048435:CDS 45.0%
! GGCGTTGAAAGACTTAGACT+TGG - chr8.3:35802470-35802489 MS.gene048435:CDS 45.0%
! TCAATGCTGCTTCTGGATAC+AGG + chr8.3:35801844-35801863 None:intergenic 45.0%
! TGGGTTGGGATGCTATGATT+AGG - chr8.3:35802403-35802422 MS.gene048435:CDS 45.0%
AAGATGTCACCGACACTGAG+TGG - chr8.3:35801101-35801120 MS.gene048435:CDS 50.0%
AATCCTTTGTCAACGGCACC+GGG - chr8.3:35801146-35801165 MS.gene048435:CDS 50.0%
ACCGAAACTCAGAATCTCCC+CGG + chr8.3:35801725-35801744 None:intergenic 50.0%
AGGTACGGTGGCAGTGTTAA+CGG + chr8.3:35801512-35801531 None:intergenic 50.0%
ATGAAATCAAGCAGCCGAGG+TGG - chr8.3:35801913-35801932 MS.gene048435:CDS 50.0%
CAAGGGCTGGAATCGTCAAA+AGG - chr8.3:35802219-35802238 MS.gene048435:CDS 50.0%
CAATCCTTTGTCAACGGCAC+CGG - chr8.3:35801145-35801164 MS.gene048435:CDS 50.0%
CATGAAAGCTTCCATGACGG+AGG + chr8.3:35800711-35800730 None:intergenic 50.0%
CGAGGTAGAAAACCGGCTAA+TGG - chr8.3:35802030-35802049 MS.gene048435:CDS 50.0%
CGCGTAGAGCGTAGAATTTC+TGG + chr8.3:35802113-35802132 None:intergenic 50.0%
GAAATCGAAGCTACAAGGGC+TGG - chr8.3:35802206-35802225 MS.gene048435:CDS 50.0%
GAAATGGTTGCGTTCATGGC+AGG + chr8.3:35801532-35801551 None:intergenic 50.0%
GAAGGAAGGAGAGAGTAGCA+GGG - chr8.3:35801978-35801997 MS.gene048435:CDS 50.0%
GAAGTTGCGGAATGTGGTGT+TGG + chr8.3:35800764-35800783 None:intergenic 50.0%
GGATTGCCAGAAAATCGCGT+AGG + chr8.3:35800894-35800913 None:intergenic 50.0%
GTCGGTGACATCTTCCTCAA+CGG + chr8.3:35801095-35801114 None:intergenic 50.0%
TCAGAGCTTGAAGACGGTGT+TGG + chr8.3:35800847-35800866 None:intergenic 50.0%
TCCGGGGAGATTCTGAGTTT+CGG - chr8.3:35801721-35801740 MS.gene048435:CDS 50.0%
TGAAGGAAGGAGAGAGTAGC+AGG - chr8.3:35801977-35801996 MS.gene048435:CDS 50.0%
TGTCTCAGATGATGCCGTTG+AGG - chr8.3:35801078-35801097 MS.gene048435:CDS 50.0%
! CGATGAGCTTGTTCTTCTGG+AGG + chr8.3:35801010-35801029 None:intergenic 50.0%
! TCCATCGTCAATGCTGCTTC+TGG + chr8.3:35801851-35801870 None:intergenic 50.0%
! TTGAAGGCGCGAAAGAAAGC+TGG - chr8.3:35800867-35800886 MS.gene048435:CDS 50.0%
!! TCCAGAAGCAGCATTGACGA+TGG - chr8.3:35801847-35801866 MS.gene048435:CDS 50.0%
AAATCAAGCAGCCGAGGTGG+AGG - chr8.3:35801916-35801935 MS.gene048435:CDS 55.0%
ATAAGCCTGGCTCAGTGACC+CGG + chr8.3:35801167-35801186 None:intergenic 55.0%
ATTCTACGCTCTACGCGCTG+TGG - chr8.3:35802116-35802135 MS.gene048435:CDS 55.0%
CTCAGAATCTCCCCGGATTC+TGG + chr8.3:35801718-35801737 None:intergenic 55.0%
GGTCAGTCACAGTTCGTTGC+CGG - chr8.3:35801793-35801812 MS.gene048435:CDS 55.0%
GGTCCATGGGTTTCAGACCT+TGG - chr8.3:35801264-35801283 MS.gene048435:CDS 55.0%
GGTTGCGTTCATGGCAGGTA+CGG + chr8.3:35801527-35801546 None:intergenic 55.0%
TACGGTGGCAGTGTTAACGG+TGG + chr8.3:35801509-35801528 None:intergenic 55.0%
TCATCCTGCGGCGAAGTTGA+TGG - chr8.3:35802446-35802465 MS.gene048435:CDS 55.0%
TCCTGAAACAGGTTCGTGGC+CGG - chr8.3:35801396-35801415 MS.gene048435:CDS 55.0%
TCTCTCTGCCTCTGCAGCTA+TGG - chr8.3:35801471-35801490 MS.gene048435:CDS 55.0%
! GCTGGACCTACGCGATTTTC+TGG - chr8.3:35800885-35800904 MS.gene048435:CDS 55.0%
! TGTTGTGATGGAGGAGGCAC+TGG + chr8.3:35800800-35800819 None:intergenic 55.0%
ACCAACGTCTTCAAGCGGGG+TGG - chr8.3:35801294-35801313 MS.gene048435:CDS 60.0%
ACCACCCCGCTTGAAGACGT+TGG + chr8.3:35801298-35801317 None:intergenic 60.0%
ACCATGGCTACACCGCTGCT+CGG - chr8.3:35800926-35800945 MS.gene048435:CDS 60.0%
ACCCATGGACCCGTGCTTCA+CGG + chr8.3:35801255-35801274 None:intergenic 60.0%
ACCGGCCACGAACCTGTTTC+AGG + chr8.3:35801400-35801419 None:intergenic 60.0%
AGTAGCAGGGTGGTTGAGCC+AGG - chr8.3:35801991-35802010 MS.gene048435:CDS 60.0%
CAGAATCTCCGCCTCCACCT+CGG + chr8.3:35801930-35801949 None:intergenic 60.0%
CGTTTCTTCGGTCGCTTCCC+TGG + chr8.3:35802012-35802031 None:intergenic 60.0%
GGAAGGAGAGAGTAGCAGGG+TGG - chr8.3:35801981-35802000 MS.gene048435:CDS 60.0%
GGACGACAACTCCTCCGTCA+TGG - chr8.3:35800697-35800716 MS.gene048435:CDS 60.0%
GGGAAGCGACCGAAGAAACG+AGG - chr8.3:35802012-35802031 MS.gene048435:CDS 60.0%
GTAGCAGGGTGGTTGAGCCA+GGG - chr8.3:35801992-35802011 MS.gene048435:CDS 60.0%
TCAACCCCGGTGTGGATCAC+CGG - chr8.3:35801193-35801212 MS.gene048435:CDS 60.0%
TGCGTTCATGGCAGGTACGG+TGG + chr8.3:35801524-35801543 None:intergenic 60.0%
TGTGGATCACCGGGGCTGAA+AGG - chr8.3:35801203-35801222 MS.gene048435:CDS 60.0%
!! TCCTGCGGCGAAGTTGATGG+CGG - chr8.3:35802449-35802468 MS.gene048435:CDS 60.0%
!! TGACCCGGTGCCGTTGACAA+AGG + chr8.3:35801152-35801171 None:intergenic 60.0%
!! TGTGATGGAGGAGGCACTGG+TGG + chr8.3:35800797-35800816 None:intergenic 60.0%
AACCCCGGTGTGGATCACCG+GGG - chr8.3:35801195-35801214 MS.gene048435:CDS 65.0%
CAACCCCGGTGTGGATCACC+GGG - chr8.3:35801194-35801213 MS.gene048435:CDS 65.0%
CCCATGGACCCGTGCTTCAC+GGG + chr8.3:35801254-35801273 None:intergenic 65.0%
CCCGTGAAGCACGGGTCCAT+GGG - chr8.3:35801251-35801270 MS.gene048435:CDS 65.0%
GTGAACGGGCCCGTGAAGCA+CGG - chr8.3:35801242-35801261 MS.gene048435:CDS 65.0%
TCAAGCAGCCGAGGTGGAGG+CGG - chr8.3:35801919-35801938 MS.gene048435:CDS 65.0%
TCAGCCCCGGTGATCCACAC+CGG + chr8.3:35801202-35801221 None:intergenic 65.0%
TGAACGGGCCCGTGAAGCAC+GGG - chr8.3:35801243-35801262 MS.gene048435:CDS 65.0%
AGCCCCGGTGATCCACACCG+GGG + chr8.3:35801200-35801219 None:intergenic 70.0%
CAGCCCCGGTGATCCACACC+GGG + chr8.3:35801201-35801220 None:intergenic 70.0%
CCATCACCCCAGCCGAGCAG+CGG + chr8.3:35800941-35800960 None:intergenic 70.0%
GCCCGTGAAGCACGGGTCCA+TGG - chr8.3:35801250-35801269 MS.gene048435:CDS 70.0%
GCCGAGCAGCGGTGTAGCCA+TGG + chr8.3:35800930-35800949 None:intergenic 70.0%
GCCGCCATCAACTTCGCCGC+AGG + chr8.3:35802453-35802472 None:intergenic 70.0%
GCTACACCGCTGCTCGGCTG+GGG - chr8.3:35800932-35800951 MS.gene048435:CDS 70.0%
GGCTACACCGCTGCTCGGCT+GGG - chr8.3:35800931-35800950 MS.gene048435:CDS 70.0%
TGGCTACACCGCTGCTCGGC+TGG - chr8.3:35800930-35800949 MS.gene048435:CDS 70.0%
CCGCTGCTCGGCTGGGGTGA+TGG - chr8.3:35800938-35800957 MS.gene048435:CDS 75.0%
! CGGCACCGGGTCACTGAGCC+AGG - chr8.3:35801159-35801178 MS.gene048435:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 35800683 35802632 35800683 ID=MS.gene048435
chr8.3 mRNA 35800683 35802632 35800683 ID=MS.gene048435.t1;Parent=MS.gene048435
chr8.3 exon 35800683 35802632 35800683 ID=MS.gene048435.t1.exon1;Parent=MS.gene048435.t1
chr8.3 CDS 35800683 35802632 35800683 ID=cds.MS.gene048435.t1;Parent=MS.gene048435.t1
Gene Sequence

>MS.gene048435

ATGAATAACATTTGGGACGACAACTCCTCCGTCATGGAAGCTTTCATGACCACCTCTGACCTCTCTAATTTCTGGCTTCCAACACCACATTCCGCAACTTCAACAACTGCACCACCAGTGCCTCCTCCATCACAACAGTCTATGTTCAATCAAGAGACTCTCCAACACCGTCTTCAAGCTCTGATTGAAGGCGCGAAAGAAAGCTGGACCTACGCGATTTTCTGGCAATCCTCTTATGATTACACCATGGCTACACCGCTGCTCGGCTGGGGTGATGGATATTACAAAGGGGAAGATGACAAAGGAAAACTGAAGCGAGTCACGCCTCCAGAAGAACAAGCTCATCGAAGAAAGATTCTCCGTGAGCTTAATACGTTGATTTCAGGTGGTTCGTCTGTCTCAGATGATGCCGTTGAGGAAGATGTCACCGACACTGAGTGGTTTTTTCTCACGTCGATGACTCAATCCTTTGTCAACGGCACCGGGTCACTGAGCCAGGCTTATTTCAATTCAACCCCGGTGTGGATCACCGGGGCTGAAAGGCTTACTAATTCTGTCTGTGAACGGGCCCGTGAAGCACGGGTCCATGGGTTTCAGACCTTGGTATGTATACCAACGTCTTCAAGCGGGGTGGTTGAGCTTGCTTCTACAGAAATGATTCCATACAACGCTGATCTAATGGAGAAGATTCGTGTTTTGTTTAATTTCAACAATCCTGAAACAGGTTCGTGGCCGGTGAATTCCATTACAACGAGCGAGAACGATCCTTCATCTGTCTGGCTCAATGATCTCTCTGCCTCTGCAGCTATGGAAATTCGAGATACCACCGTTAACACTGCCACCGTACCTGCCATGAACGCAACCATTTCTGCAAATGCAACAATCGGTAAATCATTACCGTTTGAAACAAATGGTTCAACCAGTACTCTAACGGAAACACCAACTGCAGTTAACTTTGCTCAGCGTCAGAATCAGAACAACCAGAACCAGAGCTTTTTTCTGAAGGAACTGAATTTTTCTGGTTCAATGAAACCAGAATCCGGGGAGATTCTGAGTTTCGGTGAAAGTAAGAAAAGTTCTTATATCACCGGAAATGGAACTTTCTTTTCTGGTCAGTCACAGTTCGTTGCCGGTGAAGAAAACAGAAAAAGAAAGTCTCCTGTATCCAGAAGCAGCATTGACGATGGAATGCTTTCGTTTACTTCCGGTGTAGTCTTACCGTCTTCAAATATGAAATCAAGCAGCCGAGGTGGAGGCGGAGATTCTGATCATTCAGATCTAGATGTTTCTGCTGTGAAGGAAGGAGAGAGTAGCAGGGTGGTTGAGCCAGGGAAGCGACCGAAGAAACGAGGTAGAAAACCGGCTAATGGAAGAGAAGAACCGTTGAATCATGTAGAAGCAGAGAGACAGAGAAGAGAAAAGCTTAACCAGAAATTCTACGCTCTACGCGCTGTGGTTCCAAATGTTTCCAAAATGGACAAAGCATCTCTGCTAGGCGATGCTATTTCTTATATAAACGAATTGAAATCGAAGCTACAAGGGCTGGAATCGTCAAAAGGTGAATTGGAGAAACAATTAGGTGCAACAAAGAAGGAACTAGAACATGTTGCAAGCAAAAATCAAAATCAAAATCCAATTCCATTAGATAAAGAAAAGGAGAAAACAACAAGCTCAACAAGCTCTTCTAAGTTAATTGACTTGGATATTGAAGTGAAGATTATGGGTTGGGATGCTATGATTAGGATTCAATGCAGCAAGAAGAATCATCCTGCGGCGAAGTTGATGGCGGCGTTGAAAGACTTAGACTTGGATGTTAATCATGCTAGTGTGTCTGTTGTGAATGATTTGATGATTCAACAAGCTTCTGTTAACATGGGAAGTCGATTTTACACACAAGAACAACTTTTGTCTCTTCTTTCTTCCAAAATTGGTGATGCACAAGGAGATTGA
Protein sequence

>MS.gene048435.t1

MNNIWDDNSSVMEAFMTTSDLSNFWLPTPHSATSTTAPPVPPPSQQSMFNQETLQHRLQALIEGAKESWTYAIFWQSSYDYTMATPLLGWGDGYYKGEDDKGKLKRVTPPEEQAHRRKILRELNTLISGGSSVSDDAVEEDVTDTEWFFLTSMTQSFVNGTGSLSQAYFNSTPVWITGAERLTNSVCERAREARVHGFQTLVCIPTSSSGVVELASTEMIPYNADLMEKIRVLFNFNNPETGSWPVNSITTSENDPSSVWLNDLSASAAMEIRDTTVNTATVPAMNATISANATIGKSLPFETNGSTSTLTETPTAVNFAQRQNQNNQNQSFFLKELNFSGSMKPESGEILSFGESKKSSYITGNGTFFSGQSQFVAGEENRKRKSPVSRSSIDDGMLSFTSGVVLPSSNMKSSSRGGGGDSDHSDLDVSAVKEGESSRVVEPGKRPKKRGRKPANGREEPLNHVEAERQRREKLNQKFYALRAVVPNVSKMDKASLLGDAISYINELKSKLQGLESSKGELEKQLGATKKELEHVASKNQNQNPIPLDKEKEKTTSSTSSSKLIDLDIEVKIMGWDAMIRIQCSKKNHPAAKLMAALKDLDLDVNHASVSVVNDLMIQQASVNMGSRFYTQEQLLSLLSSKIGDAQGD