AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048540.t1 MTR_4g063790 91.813 171 14 0 1 171 1 171 1.54e-112 317
MS.gene048540.t1 MTR_3g465410 93.007 143 10 0 29 171 1 143 3.61e-95 272
MS.gene048540.t1 MTR_3g467080 78.882 161 34 0 1 161 1 161 1.91e-88 256
MS.gene048540.t1 MTR_3g466980 53.488 172 77 2 1 170 1 171 2.79e-59 184
MS.gene048540.t1 MTR_3g031240 50.920 163 80 0 1 163 1 163 6.19e-54 169
MS.gene048540.t1 MTR_2g016210 45.509 167 91 0 1 167 1 167 6.07e-48 156
MS.gene048540.t1 MTR_3g065100 51.266 158 76 1 1 158 1 157 9.72e-46 150
MS.gene048540.t1 MTR_3g031100 45.509 167 91 0 1 167 1 167 8.39e-45 148
MS.gene048540.t1 MTR_5g075380 41.875 160 86 2 1 157 1 156 4.66e-42 138
MS.gene048540.t1 MTR_4g032620 40.000 165 99 0 1 165 1 165 1.41e-41 139
MS.gene048540.t1 MTR_8g036130 41.477 176 92 2 1 165 1 176 1.56e-40 137
MS.gene048540.t1 MTR_2g035580 47.097 155 81 1 1 155 1 154 3.95e-40 133
MS.gene048540.t1 MTR_4g031910 42.424 165 95 0 1 165 1 165 1.20e-37 129
MS.gene048540.t1 MTR_5g047580 42.038 157 89 2 1 157 1 155 6.19e-37 125
MS.gene048540.t1 MTR_4g032290 41.818 165 96 0 1 165 1 165 6.32e-36 125
MS.gene048540.t1 MTR_1g077390 40.881 159 92 2 1 159 1 157 3.73e-34 118
MS.gene048540.t1 MTR_3g466830 40.000 155 92 1 3 157 2 155 5.25e-34 117
MS.gene048540.t1 MTR_4g032260 38.365 159 98 0 1 159 1 159 1.35e-32 114
MS.gene048540.t1 MTR_2g035610 47.863 117 61 0 1 117 1 117 2.19e-32 112
MS.gene048540.t1 MTR_1g090783 38.158 152 92 2 3 154 2 151 1.25e-30 109
MS.gene048540.t1 MTR_4g028720 40.252 159 93 2 1 159 1 157 1.32e-30 109
MS.gene048540.t1 MTR_1g084950 40.127 157 92 2 1 157 1 155 2.06e-30 108
MS.gene048540.t1 MTR_1g077320 40.252 159 93 2 1 159 1 157 2.58e-30 108
MS.gene048540.t1 MTR_7g011950 39.623 159 94 2 1 159 1 157 9.65e-30 107
MS.gene048540.t1 MTR_1g077300 39.752 161 93 2 1 161 1 157 2.87e-29 105
MS.gene048540.t1 MTR_3g466890 36.774 155 97 1 3 157 2 155 3.23e-28 103
MS.gene048540.t1 MTR_1g090710 35.714 154 98 1 1 154 1 153 2.43e-27 100
MS.gene048540.t1 MTR_1g090697 36.538 156 93 4 1 154 1 152 6.67e-27 99.8
MS.gene048540.t1 MTR_5g047560 35.714 154 81 3 1 154 1 136 7.74e-22 86.3
MS.gene048540.t1 MTR_3g466930 34.921 126 81 1 32 157 2 126 1.07e-19 80.5
MS.gene048540.t1 MTR_4g028800 47.222 72 36 1 1 72 1 70 1.90e-19 78.2
MS.gene048540.t1 MTR_3g466900 33.333 126 83 1 32 157 2 126 5.90e-18 75.9
MS.gene048540.t1 MTR_7g055800 35.043 117 48 2 1 117 1 89 2.27e-16 70.5
MS.gene048540.t1 MTR_7g106510 30.345 145 83 4 1 139 1 133 4.78e-16 75.1
MS.gene048540.t1 MTR_4g019670 31.206 141 84 3 1 135 1 134 1.53e-15 73.6
MS.gene048540.t1 MTR_6g018920 27.500 160 101 4 1 154 1 151 1.00e-14 70.9
MS.gene048540.t1 MTR_7g055940 34.906 106 41 2 1 106 1 78 1.23e-14 65.9
MS.gene048540.t1 MTR_7g055790 37.615 109 50 3 1 109 59 149 3.16e-14 66.6
MS.gene048540.t1 MTR_3g093900 31.313 99 68 0 5 103 8 106 9.40e-14 68.6
MS.gene048540.t1 MTR_1g114730 27.044 159 101 4 1 153 1 150 6.63e-13 65.9
MS.gene048540.t1 MTR_6g005440 28.750 160 99 4 1 154 1 151 7.17e-13 65.5
MS.gene048540.t1 MTR_6g005450 28.750 160 99 4 1 154 1 151 1.56e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048540.t1 AT5G48670 51.592 157 71 3 1 155 1 154 3.44e-41 140
MS.gene048540.t1 AT3G05860 40.116 172 98 2 1 170 1 169 8.25e-34 119
MS.gene048540.t1 AT3G05860 40.116 172 98 2 1 170 1 169 1.63e-33 119
MS.gene048540.t1 AT3G05860 40.116 172 98 2 1 170 1 169 2.03e-33 119
MS.gene048540.t1 AT1G65330 41.830 153 88 1 3 155 2 153 1.08e-32 117
MS.gene048540.t1 AT1G65300 40.252 159 93 2 5 163 4 160 3.02e-30 111
MS.gene048540.t1 AT5G27810 52.809 89 42 0 29 117 1 89 4.31e-29 104
MS.gene048540.t1 AT5G26650 36.943 157 90 2 5 157 3 154 3.75e-28 107
MS.gene048540.t1 AT1G31630 36.943 157 95 3 3 158 2 155 5.59e-28 107
MS.gene048540.t1 AT5G27960 45.872 109 59 0 5 113 3 111 3.28e-27 104
MS.gene048540.t1 AT1G31640 39.506 162 84 4 3 158 2 155 1.24e-26 104
MS.gene048540.t1 AT1G22590 40.708 113 67 0 1 113 1 113 3.45e-26 98.2
MS.gene048540.t1 AT5G26630 38.312 154 94 1 1 154 1 153 4.29e-26 99.4
MS.gene048540.t1 AT2G28700 32.386 176 108 4 1 171 1 170 5.68e-21 87.8
MS.gene048540.t1 AT5G06500 29.936 157 102 3 1 154 1 152 3.71e-20 84.3
MS.gene048540.t1 AT2G40210 36.634 101 64 0 1 101 1 101 1.72e-15 73.2
MS.gene048540.t1 AT5G26580 38.235 102 49 1 1 102 1 88 8.17e-15 71.2
MS.gene048540.t1 AT5G55690 35.294 102 59 3 1 95 1 102 2.39e-11 60.8
MS.gene048540.t1 AT5G55690 35.294 102 59 3 1 95 1 102 2.39e-11 60.8

Find 39 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCATCTTTC+TGG 0.077675 3.3:+52869954 None:intergenic
ATTCAAACCATAGAATTTAA+TGG 0.173791 3.3:-52869916 MS.gene048540:CDS
CGAATTTCCATTAAATTCTA+TGG 0.218755 3.3:+52869909 None:intergenic
GAGGTTTGGCCATCAGATTC+AGG 0.258145 3.3:-52870111 MS.gene048540:CDS
CGCTACCATGACTAGAAATA+AGG 0.322931 3.3:-52870508 None:intergenic
CAAGAGGAGGTTGGAAATGA+GGG 0.355119 3.3:-52869793 MS.gene048540:CDS
TCTTCTTGAGGCAAAACATT+AGG 0.355934 3.3:-52870014 MS.gene048540:CDS
AATATAATGATCAATGATAT+TGG 0.368342 3.3:+52869936 None:intergenic
GAAGTGTGATATCTAGGTTT+AGG 0.426765 3.3:-52870083 MS.gene048540:CDS
TCAAGAGGAGGTTGGAAATG+AGG 0.431693 3.3:-52869794 MS.gene048540:CDS
GGGTCAGAAGTGTGATATCT+AGG 0.436600 3.3:-52870089 MS.gene048540:CDS
TCTAGGTTTAGGAGTTTGCC+TGG 0.445921 3.3:-52870072 MS.gene048540:CDS
AGAATTTGAAGGAGGTTGAT+CGG 0.478161 3.3:-52869843 MS.gene048540:CDS
ACAACCCTCGAGTAGAGGTT+TGG 0.487846 3.3:-52870125 MS.gene048540:CDS
AGGTTTGGCCATCAGATTCA+GGG 0.511068 3.3:-52870110 MS.gene048540:CDS
ACGAGCAGTTGAAGAAGCAA+AGG 0.512962 3.3:-52869984 MS.gene048540:CDS
AAGGGAGGAAACCAGAAAGA+TGG 0.513695 3.3:-52869965 MS.gene048540:CDS
TTTCACCTTATTTCTAGTCA+TGG 0.525434 3.3:+52870503 None:intergenic
GGAGGATAACATTCAGAAAG+AGG 0.525464 3.3:-52870452 MS.gene048540:CDS
ATCAAAGATCAAGAGGAGGT+TGG 0.534167 3.3:-52869802 MS.gene048540:CDS
AATGACAATCAAAGATCAAG+AGG 0.572865 3.3:-52869809 MS.gene048540:CDS
CGAGCAGTTGAAGAAGCAAA+GGG 0.580872 3.3:-52869983 MS.gene048540:CDS
ACATGCTTCGACTCCACATA+GGG 0.581479 3.3:+52870167 None:intergenic
AAGAGGAGGTTGGAAATGAG+GGG 0.584160 3.3:-52869792 MS.gene048540:CDS
TATTGATGAGAATTTGAAGG+AGG 0.592173 3.3:-52869851 MS.gene048540:CDS
CACATGCTTCGACTCCACAT+AGG 0.603214 3.3:+52870166 None:intergenic
AATGAAATTAGCACCCTATG+TGG 0.604835 3.3:-52870180 MS.gene048540:CDS
AGAGGAGGTTGGAAATGAGG+GGG 0.608627 3.3:-52869791 MS.gene048540:CDS
GCAAAACAACCCTCGAGTAG+AGG 0.609086 3.3:-52870130 MS.gene048540:CDS
CTTCTGACCCCTGAATCTGA+TGG 0.624024 3.3:+52870102 None:intergenic
ACATAACAAGTGACTCGAAG+AGG 0.633454 3.3:-52870473 MS.gene048540:CDS
GATGGCCAAACCTCTACTCG+AGG 0.634480 3.3:+52870120 None:intergenic
GAGGAGGTTGGAAATGAGGG+GGG 0.643505 3.3:-52869790 MS.gene048540:CDS
GACAATCAAAGATCAAGAGG+AGG 0.656411 3.3:-52869806 MS.gene048540:CDS
GGTTTGGCCATCAGATTCAG+GGG 0.657992 3.3:-52870109 MS.gene048540:CDS
ATGGCCAAACCTCTACTCGA+GGG 0.659257 3.3:+52870121 None:intergenic
TAACAAGTGACTCGAAGAGG+AGG 0.659370 3.3:-52870470 MS.gene048540:CDS
GCAGTTGAAGAAGCAAAGGG+AGG 0.693727 3.3:-52869980 MS.gene048540:CDS
TGGATCAGGAGGTCTTCTTG+AGG 0.700337 3.3:-52870026 MS.gene048540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATATACATTTTTTTTAAT+AGG - chr3.3:52870065-52870084 MS.gene048540:CDS 10.0%
!! AATAAACAATATATAATCGA+TGG + chr3.3:52870046-52870065 None:intergenic 15.0%
!! AATATAATGATCAATGATAT+TGG + chr3.3:52870351-52870370 None:intergenic 15.0%
!! ATTCAAACCATAGAATTTAA+TGG - chr3.3:52870368-52870387 MS.gene048540:intron 20.0%
!!! TTTTATTGATGAGAATTTGA+AGG - chr3.3:52870430-52870449 MS.gene048540:intron 20.0%
! ACATGAACAAAGTAAAAAGA+TGG - chr3.3:52870235-52870254 MS.gene048540:intron 25.0%
! CGAATTTCCATTAAATTCTA+TGG + chr3.3:52870378-52870397 None:intergenic 25.0%
! TGTTAACATGTGATATTTCT+AGG + chr3.3:52869941-52869960 None:intergenic 25.0%
!! TACATGCTTTGTTGAAATTA+TGG + chr3.3:52869881-52869900 None:intergenic 25.0%
!!! TGTGATATTTCTAGGAAAAA+AGG + chr3.3:52869933-52869952 None:intergenic 25.0%
AATGACAATCAAAGATCAAG+AGG - chr3.3:52870475-52870494 MS.gene048540:CDS 30.0%
ACATTCAGAAAGAGGAAAAA+TGG - chr3.3:52869840-52869859 MS.gene048540:CDS 30.0%
TATTGATGAGAATTTGAAGG+AGG - chr3.3:52870433-52870452 MS.gene048540:intron 30.0%
TGAACAAAGTAAAAAGATGG+TGG - chr3.3:52870238-52870257 MS.gene048540:intron 30.0%
! TTTTTACTTTGTTCATGTCC+AGG + chr3.3:52870233-52870252 None:intergenic 30.0%
AATGAAATTAGCACCCTATG+TGG - chr3.3:52870104-52870123 MS.gene048540:CDS 35.0%
ATTGGTCATTTCCATCTTTC+TGG + chr3.3:52870333-52870352 None:intergenic 35.0%
GAAGTGTGATATCTAGGTTT+AGG - chr3.3:52870201-52870220 MS.gene048540:intron 35.0%
TCTTCTTGAGGCAAAACATT+AGG - chr3.3:52870270-52870289 MS.gene048540:intron 35.0%
! AGAATTTGAAGGAGGTTGAT+CGG - chr3.3:52870441-52870460 MS.gene048540:intron 35.0%
! GCTTTGTTGAAATTATGGAG+AGG + chr3.3:52869876-52869895 None:intergenic 35.0%
!! AAGTAAAAAGATGGTGGATC+AGG - chr3.3:52870244-52870263 MS.gene048540:intron 35.0%
ACATAACAAGTGACTCGAAG+AGG - chr3.3:52869811-52869830 MS.gene048540:CDS 40.0%
ATCAAAGATCAAGAGGAGGT+TGG - chr3.3:52870482-52870501 MS.gene048540:CDS 40.0%
GACAATCAAAGATCAAGAGG+AGG - chr3.3:52870478-52870497 MS.gene048540:CDS 40.0%
GGAGGATAACATTCAGAAAG+AGG - chr3.3:52869832-52869851 MS.gene048540:CDS 40.0%
!! TAAAAAGATGGTGGATCAGG+AGG - chr3.3:52870247-52870266 MS.gene048540:intron 40.0%
AAGAGGAGGTTGGAAATGAG+GGG - chr3.3:52870492-52870511 MS.gene048540:CDS 45.0%
AAGGGAGGAAACCAGAAAGA+TGG - chr3.3:52870319-52870338 MS.gene048540:intron 45.0%
ACATGCTTCGACTCCACATA+GGG + chr3.3:52870120-52870139 None:intergenic 45.0%
ACGAGCAGTTGAAGAAGCAA+AGG - chr3.3:52870300-52870319 MS.gene048540:intron 45.0%
AGGTTTGGCCATCAGATTCA+GGG - chr3.3:52870174-52870193 MS.gene048540:CDS 45.0%
CAAGAGGAGGTTGGAAATGA+GGG - chr3.3:52870491-52870510 MS.gene048540:CDS 45.0%
CGAGCAGTTGAAGAAGCAAA+GGG - chr3.3:52870301-52870320 MS.gene048540:intron 45.0%
TAACAAGTGACTCGAAGAGG+AGG - chr3.3:52869814-52869833 MS.gene048540:CDS 45.0%
TCAAGAGGAGGTTGGAAATG+AGG - chr3.3:52870490-52870509 MS.gene048540:CDS 45.0%
TCTAGGTTTAGGAGTTTGCC+TGG - chr3.3:52870212-52870231 MS.gene048540:intron 45.0%
! GGGTCAGAAGTGTGATATCT+AGG - chr3.3:52870195-52870214 MS.gene048540:CDS 45.0%
ACAACCCTCGAGTAGAGGTT+TGG - chr3.3:52870159-52870178 MS.gene048540:CDS 50.0%
AGAGGAGGTTGGAAATGAGG+GGG - chr3.3:52870493-52870512 MS.gene048540:CDS 50.0%
ATGGCCAAACCTCTACTCGA+GGG + chr3.3:52870166-52870185 None:intergenic 50.0%
CACATGCTTCGACTCCACAT+AGG + chr3.3:52870121-52870140 None:intergenic 50.0%
CTTCTGACCCCTGAATCTGA+TGG + chr3.3:52870185-52870204 None:intergenic 50.0%
GAGGTTTGGCCATCAGATTC+AGG - chr3.3:52870173-52870192 MS.gene048540:CDS 50.0%
GCAAAACAACCCTCGAGTAG+AGG - chr3.3:52870154-52870173 MS.gene048540:CDS 50.0%
GCAGTTGAAGAAGCAAAGGG+AGG - chr3.3:52870304-52870323 MS.gene048540:intron 50.0%
GGTTTGGCCATCAGATTCAG+GGG - chr3.3:52870175-52870194 MS.gene048540:CDS 50.0%
TGGATCAGGAGGTCTTCTTG+AGG - chr3.3:52870258-52870277 MS.gene048540:intron 50.0%
GAGGAGGTTGGAAATGAGGG+GGG - chr3.3:52870494-52870513 MS.gene048540:CDS 55.0%
GATGGCCAAACCTCTACTCG+AGG + chr3.3:52870167-52870186 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 52869783 52870523 52869783 ID=MS.gene048540
chr3.3 mRNA 52869783 52870523 52869783 ID=MS.gene048540.t1;Parent=MS.gene048540
chr3.3 exon 52870445 52870523 52870445 ID=MS.gene048540.t1.exon1;Parent=MS.gene048540.t1
chr3.3 CDS 52870445 52870523 52870445 ID=cds.MS.gene048540.t1;Parent=MS.gene048540.t1
chr3.3 exon 52869783 52870219 52869783 ID=MS.gene048540.t1.exon2;Parent=MS.gene048540.t1
chr3.3 CDS 52869783 52870219 52869783 ID=cds.MS.gene048540.t1;Parent=MS.gene048540.t1
Gene Sequence

>MS.gene048540

ATGACTAGAAATAAGGTGAAACTAGCATACATAACAAGTGACTCGAAGAGGAGGATAACATTCAGAAAGAGGAAAAATGGTTAGATTTAACCTCTCCATAATTTCAACAAAGCATGTATATATTATCTTTGCATATCATACACATAGCCTTTTTTCCTAGAAATATCACATGTTAACATTTTTTTTATATTTTTTAAAACTATAATTTATAGTTTTGAAAATTCGTAATTTAGATTTCATTTAGTAAATTTGAAATTTTACCATCGATTATATATTGTTTATTGATATACATTTTTTTTAATAGGAATTATGAAGAAGATTAATGAAATTAGCACCCTATGTGGAGTCGAAGCATGTGCAATAATTTCAGAGCAAAACAACCCTCGAGTAGAGGTTTGGCCATCAGATTCAGGGGTCAGAAGTGTGATATCTAGGTTTAGGAGTTTGCCTGGACATGAACAAAGTAAAAAGATGGTGGATCAGGAGGTCTTCTTGAGGCAAAACATTAGGAAAGTCTACGAGCAGTTGAAGAAGCAAAGGGAGGAAACCAGAAAGATGGAAATGACCAATATCATTGATCATTATATTCAAACCATAGAATTTAATGGAAATTCGATGAGCAAATCTGATCTTAATGATTTTTCTTCTTTTATTGATGAGAATTTGAAGGAGGTTGATCGGAAGATGAAAGAAATGACAATCAAAGATCAAGAGGAGGTTGGAAATGAGGGGGGATTATGA

Protein sequence

>MS.gene048540.t1

MTRNKVKLAYITSDSKRRITFRKRKNGIMKKINEISTLCGVEACAIISEQNNPRVEVWPSDSGVRSVISRFRSLPGHEQSKKMVDQEVFLRQNIRKVYEQLKKQREETRKMEMTNIIDHYIQTIEFNGNSMSKSDLNDFSSFIDENLKEVDRKMKEMTIKDQEEVGNEGGL