AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene04858

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04858.t1 MTR_7g093010 91.185 329 18 3 1 327 1 320 0.0 594
MS.gene04858.t1 MTR_1g053800 40.199 301 145 8 35 327 9 282 5.18e-48 164
MS.gene04858.t1 MTR_1g053835 41.667 144 76 2 191 327 220 362 1.00e-22 98.2
MS.gene04858.t1 MTR_7g093030 47.170 106 56 0 222 327 212 317 8.68e-22 95.1
MS.gene04858.t1 MTR_1g080330 41.026 117 68 1 212 327 165 281 1.02e-19 87.8
MS.gene04858.t1 MTR_1g080330 41.026 117 68 1 212 327 165 281 2.99e-19 87.8
MS.gene04858.t1 MTR_7g098250 39.041 146 75 4 190 327 229 368 3.76e-19 88.2
MS.gene04858.t1 MTR_7g115530 41.121 107 62 1 222 327 218 324 1.43e-17 82.4
MS.gene04858.t1 MTR_7g093030 50.000 82 41 0 222 303 212 293 3.73e-17 80.9
MS.gene04858.t1 MTR_7g115530 41.121 107 62 1 222 327 218 324 4.35e-17 82.0
MS.gene04858.t1 MTR_7g088070 32.258 124 84 0 204 327 32 155 1.49e-16 79.0
MS.gene04858.t1 MTR_2g027860 41.593 113 55 3 222 327 49 157 1.53e-16 79.3
MS.gene04858.t1 MTR_7g088070 33.333 132 76 2 204 327 32 159 2.39e-16 78.2
MS.gene04858.t1 MTR_7g115530 41.237 97 44 2 222 318 218 301 1.86e-13 70.1
MS.gene04858.t1 MTR_5g027440 36.667 120 61 2 222 327 25 143 2.08e-13 69.7
MS.gene04858.t1 MTR_8g086410 51.667 60 29 0 224 283 113 172 5.48e-13 69.7
MS.gene04858.t1 MTR_8g086410 60.377 53 21 0 224 276 113 165 7.77e-13 69.3
MS.gene04858.t1 MTR_8g086410 60.377 53 21 0 224 276 111 163 7.95e-13 69.3
MS.gene04858.t1 MTR_2g027800 37.736 106 65 1 222 327 39 143 1.21e-12 67.8
MS.gene04858.t1 MTR_6g444980 38.017 121 59 4 222 327 35 154 1.47e-12 68.2
MS.gene04858.t1 MTR_7g115530 53.571 56 26 0 222 277 218 273 1.54e-12 67.8
MS.gene04858.t1 MTR_2g027800 57.407 54 23 0 222 275 39 92 1.62e-12 67.4
MS.gene04858.t1 MTR_1g053830 42.391 92 47 3 206 296 1 87 1.78e-12 63.2
MS.gene04858.t1 MTR_6g444980 38.017 121 59 4 222 327 35 154 2.19e-12 67.4
MS.gene04858.t1 MTR_7g115530 55.769 52 23 0 222 273 218 269 3.75e-12 66.2
MS.gene04858.t1 MTR_2g023580 43.590 78 40 1 199 276 36 109 5.60e-12 65.1
MS.gene04858.t1 MTR_6g032990 32.836 134 63 3 221 327 41 174 9.01e-12 65.9
MS.gene04858.t1 MTR_1g090670 55.556 54 24 0 222 275 44 97 1.06e-11 65.1
MS.gene04858.t1 MTR_3g450310 53.704 54 25 0 224 277 21 74 1.17e-11 63.5
MS.gene04858.t1 MTR_2g084230 56.604 53 23 0 224 276 99 151 1.43e-11 64.7
MS.gene04858.t1 MTR_2g084230 56.604 53 23 0 224 276 99 151 1.86e-11 64.7
MS.gene04858.t1 MTR_7g115530 54.902 51 23 0 222 272 218 268 2.21e-11 63.9
MS.gene04858.t1 MTR_0223s0040 45.714 70 37 1 224 293 20 88 3.20e-11 63.5
MS.gene04858.t1 MTR_7g069660 56.604 53 23 0 224 276 73 125 3.75e-11 63.9
MS.gene04858.t1 MTR_7g068600 37.069 116 61 4 220 327 11 122 4.47e-11 62.4
MS.gene04858.t1 MTR_2g016220 54.717 53 24 0 224 276 91 143 5.03e-11 63.5
MS.gene04858.t1 MTR_2g016220 54.717 53 24 0 224 276 91 143 5.04e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene04858.t1 AT5G06800 44.643 112 60 1 218 327 186 297 2.06e-20 91.3
MS.gene04858.t1 AT5G06800 45.299 117 57 2 218 327 186 302 9.14e-20 89.4
MS.gene04858.t1 AT2G01060 38.710 124 66 2 210 327 3 122 2.29e-18 84.0
MS.gene04858.t1 AT5G29000 39.837 123 68 3 210 327 219 340 8.60e-18 84.0
MS.gene04858.t1 AT5G29000 39.837 123 68 3 210 327 176 297 1.18e-17 83.2
MS.gene04858.t1 AT5G29000 39.837 123 68 3 210 327 176 297 1.18e-17 83.2
MS.gene04858.t1 AT3G04450 40.708 113 61 2 220 327 236 347 1.65e-17 83.2
MS.gene04858.t1 AT3G04450 40.708 113 61 2 220 327 196 307 2.19e-17 82.8
MS.gene04858.t1 AT3G04450 40.708 113 61 2 220 327 196 307 2.19e-17 82.8
MS.gene04858.t1 AT4G28610 40.625 128 71 3 202 327 208 332 1.03e-16 80.9
MS.gene04858.t1 AT3G13040 30.112 269 133 11 98 327 100 352 2.60e-16 79.7
MS.gene04858.t1 AT3G13040 30.112 269 133 11 98 327 100 352 2.60e-16 79.7
MS.gene04858.t1 AT3G13040 30.112 269 133 11 98 327 100 352 2.60e-16 79.7
MS.gene04858.t1 AT5G06800 61.667 60 21 1 218 275 186 245 1.02e-15 76.6
MS.gene04858.t1 AT5G29000 48.529 68 35 0 210 277 219 286 3.92e-14 72.4
MS.gene04858.t1 AT3G04450 51.724 58 28 0 220 277 236 293 1.20e-13 70.9
MS.gene04858.t1 AT2G42660 60.377 53 21 0 224 276 52 104 1.64e-13 69.7
MS.gene04858.t1 AT3G12730 54.098 61 28 0 222 282 23 83 2.49e-13 68.9
MS.gene04858.t1 AT1G79430 37.190 121 60 4 222 327 34 153 5.36e-13 69.3
MS.gene04858.t1 AT2G06020 31.387 137 80 3 147 275 9 139 1.19e-12 67.8
MS.gene04858.t1 AT4G13640 35.294 119 64 3 222 327 37 155 2.75e-12 66.6
MS.gene04858.t1 AT2G20400 38.393 112 58 2 222 327 231 337 2.95e-12 67.4
MS.gene04858.t1 AT4G04605 47.619 63 32 1 224 286 16 77 2.98e-12 65.5
MS.gene04858.t1 AT4G13640 35.294 119 64 3 222 327 37 155 3.11e-12 66.6
MS.gene04858.t1 AT3G24120 35.294 119 64 2 222 327 41 159 7.19e-12 65.5
MS.gene04858.t1 AT2G20400 38.393 112 59 2 222 327 231 338 7.33e-12 66.2
MS.gene04858.t1 AT3G24120 35.294 119 64 2 222 327 41 159 8.72e-12 65.5
MS.gene04858.t1 AT3G04030 33.333 126 62 3 221 327 44 166 9.52e-12 65.9
MS.gene04858.t1 AT3G04030 33.333 126 62 3 221 327 44 166 1.10e-11 65.5
MS.gene04858.t1 AT2G40260 54.717 53 24 0 224 276 84 136 1.12e-11 65.9
MS.gene04858.t1 AT5G18240 32.520 123 67 2 221 327 44 166 1.96e-11 65.1
MS.gene04858.t1 AT5G18240 32.520 123 67 2 221 327 44 166 1.96e-11 65.1
MS.gene04858.t1 AT5G18240 32.520 123 67 2 221 327 44 166 2.49e-11 64.7
MS.gene04858.t1 AT5G18240 32.520 123 67 2 221 327 44 166 2.52e-11 64.7
MS.gene04858.t1 AT5G18240 32.520 123 67 2 221 327 44 166 2.52e-11 64.7
MS.gene04858.t1 AT1G14600 45.455 77 38 1 205 277 2 78 2.75e-11 63.5
MS.gene04858.t1 AT2G38300 56.604 53 23 0 224 276 56 108 4.77e-11 63.5
MS.gene04858.t1 AT4G04555 54.902 51 23 0 226 276 18 68 6.04e-11 59.3
MS.gene04858.t1 AT2G02060 50.877 57 28 0 224 280 31 87 7.94e-11 62.0
MS.gene04858.t1 AT2G02060 50.877 57 28 0 224 280 31 87 7.94e-11 62.0

Find 57 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTAACAATTCTGCTTCT+AGG 0.211425 7.1:+12640471 MS.gene04858:CDS
AAGCAGACTCCTCAACTTAA+TGG 0.254270 7.1:+12640437 MS.gene04858:CDS
GAGGATGAGATTGAAGTTTC+TGG 0.281912 7.1:+12639320 MS.gene04858:CDS
AAGTATTTCGCAACAGTTAG+AGG 0.311657 7.1:+12641208 MS.gene04858:CDS
CAAACATCTCATGATGGTTT+TGG 0.318369 7.1:+12639236 MS.gene04858:CDS
ATTGGAGGGTCTGTTATCTT+TGG 0.328987 7.1:-12639362 None:intergenic
TCATGTTAAAAGCCATTTGC+AGG 0.345777 7.1:+12640713 MS.gene04858:CDS
ACGCATGCATAAAGCTTTGC+AGG 0.361361 7.1:+12640860 MS.gene04858:CDS
GTGATTCCTCAATTTGCTTA+AGG 0.361437 7.1:-12641160 None:intergenic
TTCAAGGGGCTTCTCATCAA+TGG 0.375794 7.1:-12639591 None:intergenic
AACAACCTAGTTAAGGTTGT+TGG 0.392826 7.1:+12639125 MS.gene04858:CDS
TTCTCTGAAAAGAAATTTCA+AGG 0.402613 7.1:-12639607 None:intergenic
TAGGTTGTTAATTCCTATGT+AGG 0.408171 7.1:-12639111 None:intergenic
AGGAATTAACAACCTAGTTA+AGG 0.413307 7.1:+12639118 MS.gene04858:CDS
TGTAGTGACAATGTTGTTGC+AGG 0.413465 7.1:-12639201 None:intergenic
TGTGTTCTGATCCAACAATT+TGG 0.429748 7.1:-12639154 None:intergenic
ACAACCTAGTTAAGGTTGTT+GGG 0.437913 7.1:+12639126 MS.gene04858:CDS
CAACATTGGTCAAAGATGTA+TGG 0.453490 7.1:+12639074 MS.gene04858:CDS
GTTGGGTCAGACCAAATTGT+TGG 0.464581 7.1:+12639143 MS.gene04858:CDS
CATACATCTTTGACCAATGT+TGG 0.474221 7.1:-12639073 None:intergenic
CAAAGATGTATGGAACTCAA+TGG 0.475646 7.1:+12639084 MS.gene04858:CDS
TGTAAAATTCTCTCCTCCAT+AGG 0.496495 7.1:-12639399 None:intergenic
AAAGATGTATGGAACTCAAT+GGG 0.496984 7.1:+12639085 MS.gene04858:CDS
TCTGTTCTATGTCCTTCCTC+AGG 0.498706 7.1:-12640982 None:intergenic
ATGCTGAATTGTCAAGCCTA+TGG 0.501729 7.1:+12639383 MS.gene04858:CDS
CTTTGACCAATGTTGGTACA+TGG 0.506794 7.1:-12639066 None:intergenic
TTGTTGCAGGGTGACTTGAT+TGG 0.508916 7.1:-12639188 None:intergenic
CAAGGTCAAACATCTCATGA+TGG 0.511209 7.1:+12639230 MS.gene04858:CDS
CTGACCCAACAACCTTAACT+AGG 0.516049 7.1:-12639130 None:intergenic
TAGCAGCCTTAAGCAAATTG+AGG 0.522396 7.1:+12641154 MS.gene04858:intron
GCTTGACAATTCAGCATTGG+AGG 0.527222 7.1:-12639377 None:intergenic
CTCTGAAAAGAAATTTCAAG+GGG 0.530710 7.1:-12639605 None:intergenic
CATGTAAAAGAAGAGTAAGA+TGG 0.531937 7.1:+12640504 MS.gene04858:CDS
TCTCTGAAAAGAAATTTCAA+GGG 0.533832 7.1:-12639606 None:intergenic
TAGGCTTGACAATTCAGCAT+TGG 0.547239 7.1:-12639380 None:intergenic
AGATTCATCAACAGAGTACA+AGG 0.551613 7.1:-12639441 None:intergenic
AATTGCAGAGGCAAAGCCTA+AGG 0.561046 7.1:+12640650 MS.gene04858:intron
ATGATAATCGTTGATCACCT+TGG 0.567335 7.1:+12640551 MS.gene04858:CDS
ATCATATCTAGTATAGCCTT+AGG 0.576046 7.1:-12640666 None:intergenic
ACTCAATGGGAATCCTACAT+AGG 0.576742 7.1:+12639098 MS.gene04858:CDS
AGAAGCAGAATTGTTAGAAG+TGG 0.580769 7.1:-12640468 None:intergenic
CTTGACAATTCAGCATTGGA+GGG 0.587253 7.1:-12639376 None:intergenic
ATAATCGTTGATCACCTTGG+TGG 0.592004 7.1:+12640554 MS.gene04858:CDS
ATGAGATTGAAGTTTCTGGT+TGG 0.603059 7.1:+12639324 MS.gene04858:CDS
AACCAACCATGTACCAACAT+TGG 0.603963 7.1:+12639060 None:intergenic
TTAAGCAAATTGAGGAATCA+CGG 0.605256 7.1:+12641162 MS.gene04858:CDS
GCACACATTCCATTAAGTTG+AGG 0.605770 7.1:-12640446 None:intergenic
CGCATGCATAAAGCTTTGCA+GGG 0.606639 7.1:+12640861 MS.gene04858:CDS
CTGAATTGTCAAGCCTATGG+AGG 0.616055 7.1:+12639386 MS.gene04858:CDS
AAGAAGAGTAAGATGGACAG+AGG 0.623246 7.1:+12640511 MS.gene04858:CDS
ATCACGGCAGTTGCATCTAG+AGG 0.629262 7.1:+12641178 MS.gene04858:CDS
CTTTCAGAAAGACCTGAGGA+AGG 0.638784 7.1:+12640970 MS.gene04858:intron
AGGTGAAGAATATTATGATG+AGG 0.647413 7.1:+12639301 MS.gene04858:CDS
GTAGTGACAATGTTGTTGCA+GGG 0.658470 7.1:-12639200 None:intergenic
CAAGGAGTTTGTGAACTGAG+AGG 0.666646 7.1:-12639423 None:intergenic
TTATCTTTCAGAAAGACCTG+AGG 0.675030 7.1:+12640966 MS.gene04858:intron
ACTTACTTTCTGGACCACCA+AGG 0.699661 7.1:-12640568 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACTAAAAAGAAAATCAA+TGG + chr7.1:12640120-12640139 MS.gene04858:intron 15.0%
!! AAATTATTGAAATTGTTGAA+TGG - chr7.1:12640750-12640769 None:intergenic 15.0%
!! AGGAAAATAAACAAATAAAA+AGG - chr7.1:12640360-12640379 None:intergenic 15.0%
!! ATCTAATACTATAATTAAAC+AGG - chr7.1:12639799-12639818 None:intergenic 15.0%
!! GAATCTAATTATGAAATTTA+GGG + chr7.1:12639983-12640002 MS.gene04858:intron 15.0%
!! TCTAATAAAATTGATTAAAG+TGG + chr7.1:12640064-12640083 MS.gene04858:intron 15.0%
!! TGAATCTAATTATGAAATTT+AGG + chr7.1:12639982-12640001 MS.gene04858:intron 15.0%
!! TTACTAGTAATCTATTTATT+CGG + chr7.1:12640270-12640289 MS.gene04858:intron 15.0%
!!! TCATGGATTTTTTTTTTTAA+TGG + chr7.1:12639845-12639864 MS.gene04858:intron 15.0%
!! AAAAACATGATGAATTGTTT+AGG + chr7.1:12639558-12639577 MS.gene04858:CDS 20.0%
!! AAATCCATGAAATACTTAAA+AGG - chr7.1:12639835-12639854 None:intergenic 20.0%
!! AATTAGATTCATTGCATATA+TGG - chr7.1:12639973-12639992 None:intergenic 20.0%
!! CATTTCATATATAATTCAGA+TGG - chr7.1:12639688-12639707 None:intergenic 20.0%
!! TACTAGTAATCTATTTATTC+GGG + chr7.1:12640271-12640290 MS.gene04858:intron 20.0%
!! TTAGTTAATTACTTACTTTC+TGG - chr7.1:12640581-12640600 None:intergenic 20.0%
!!! AAAAGTAATGTTAGTTTTCA+AGG + chr7.1:12639281-12639300 MS.gene04858:CDS 20.0%
!!! AACAATTCATCATGTTTTTT+CGG - chr7.1:12639557-12639576 None:intergenic 20.0%
!!! AAGAATACTGTAACTTTTTT+TGG - chr7.1:12639763-12639782 None:intergenic 20.0%
!!! ACTAACATTACTTTTGATTT+TGG - chr7.1:12639276-12639295 None:intergenic 20.0%
!!! AGTTGGGAATTTTTTTTTAA+AGG + chr7.1:12640164-12640183 MS.gene04858:intron 20.0%
!!! TCACATAAACATTCTTTTAA+CGG + chr7.1:12640087-12640106 MS.gene04858:intron 20.0%
! ATTAGAAAAACACGTTAGAT+TGG - chr7.1:12640050-12640069 None:intergenic 25.0%
! TGGATTATAGCTTATTAGAA+AGG - chr7.1:12640380-12640399 None:intergenic 25.0%
!! AATTTCTTTTCAGAGAACTA+AGG + chr7.1:12639613-12639632 MS.gene04858:CDS 25.0%
!! ATTGTCACTACAAATTTTCA+AGG + chr7.1:12639212-12639231 MS.gene04858:CDS 25.0%
!! GAATATAGTAATTTTCAGTC+TGG + chr7.1:12639506-12639525 MS.gene04858:CDS 25.0%
!! TCTCTGAAAAGAAATTTCAA+GGG - chr7.1:12639609-12639628 None:intergenic 25.0%
!! TTCTCTGAAAAGAAATTTCA+AGG - chr7.1:12639610-12639629 None:intergenic 25.0%
!!! AAATTTAGGGTTTTGTGAAA+GGG + chr7.1:12639996-12640015 MS.gene04858:intron 25.0%
!!! TAGTCCTTTTAAGTATTTCA+TGG + chr7.1:12639828-12639847 MS.gene04858:intron 25.0%
!!! TTTTTTTCTCTTCTGATTAC+TGG + chr7.1:12641096-12641115 MS.gene04858:intron 25.0%
AAAGATGTATGGAACTCAAT+GGG + chr7.1:12639085-12639104 MS.gene04858:CDS 30.0%
AAAGGGACAATAATGAATTG+CGG + chr7.1:12640013-12640032 MS.gene04858:intron 30.0%
AAGTTATGGGACTTATAGTT+GGG + chr7.1:12640148-12640167 MS.gene04858:intron 30.0%
ACAACACTGCTTACTTATTA+AGG + chr7.1:12640912-12640931 MS.gene04858:intron 30.0%
ACAATGTTAGAGTCAATTAC+CGG - chr7.1:12640802-12640821 None:intergenic 30.0%
AGGAATTAACAACCTAGTTA+AGG + chr7.1:12639118-12639137 MS.gene04858:CDS 30.0%
AGGTGAAGAATATTATGATG+AGG + chr7.1:12639301-12639320 MS.gene04858:CDS 30.0%
ATCATATCTAGTATAGCCTT+AGG - chr7.1:12640669-12640688 None:intergenic 30.0%
CATGTAAAAGAAGAGTAAGA+TGG + chr7.1:12640504-12640523 MS.gene04858:CDS 30.0%
TAGGTTGTTAATTCCTATGT+AGG - chr7.1:12639114-12639133 None:intergenic 30.0%
TGGTTTCATCTATTTGTGAA+TGG + chr7.1:12639865-12639884 MS.gene04858:intron 30.0%
TTAAGCAAATTGAGGAATCA+CGG + chr7.1:12641162-12641181 MS.gene04858:CDS 30.0%
! AAACATTCTTTTAACGGAGT+TGG + chr7.1:12640093-12640112 MS.gene04858:intron 30.0%
! ATGGCTTTTAACATGAGAAA+TGG - chr7.1:12640709-12640728 None:intergenic 30.0%
! CTCTGAAAAGAAATTTCAAG+GGG - chr7.1:12639608-12639627 None:intergenic 30.0%
! CTTACTCTTCTTTTACATGT+TGG - chr7.1:12640503-12640522 None:intergenic 30.0%
! TTCACTGATTATAATTGCAG+AGG + chr7.1:12640638-12640657 MS.gene04858:intron 30.0%
!! GAAATAGTTTTACCTGCAAA+TGG - chr7.1:12640728-12640747 None:intergenic 30.0%
!! GAAATTTAGGGTTTTGTGAA+AGG + chr7.1:12639995-12640014 MS.gene04858:intron 30.0%
AACAACCTAGTTAAGGTTGT+TGG + chr7.1:12639125-12639144 MS.gene04858:CDS 35.0%
AATCAATGGTGTCCAAGTTA+TGG + chr7.1:12640134-12640153 MS.gene04858:intron 35.0%
ACAACCTAGTTAAGGTTGTT+GGG + chr7.1:12639126-12639145 MS.gene04858:CDS 35.0%
ACACTGCTTACTTATTAAGG+AGG + chr7.1:12640915-12640934 MS.gene04858:intron 35.0%
AGAAGCAGAATTGTTAGAAG+TGG - chr7.1:12640471-12640490 None:intergenic 35.0%
AGATTCATCAACAGAGTACA+AGG - chr7.1:12639444-12639463 None:intergenic 35.0%
ATCAATGGTGTCCAAGTTAT+GGG + chr7.1:12640135-12640154 MS.gene04858:intron 35.0%
ATCCATGACTTACTATAGTC+AGG + chr7.1:12640395-12640414 MS.gene04858:intron 35.0%
ATGATAATCGTTGATCACCT+TGG + chr7.1:12640551-12640570 MS.gene04858:CDS 35.0%
ATTTCTCCAACTCGTCAATT+AGG + chr7.1:12640319-12640338 MS.gene04858:intron 35.0%
CAAAGATGTATGGAACTCAA+TGG + chr7.1:12639084-12639103 MS.gene04858:CDS 35.0%
CAACATTGGTCAAAGATGTA+TGG + chr7.1:12639074-12639093 MS.gene04858:CDS 35.0%
CAACTATAAGTCCCATAACT+TGG - chr7.1:12640149-12640168 None:intergenic 35.0%
CAAGTTATGGGACTTATAGT+TGG + chr7.1:12640147-12640166 MS.gene04858:intron 35.0%
CAATATCCTAATTGACGAGT+TGG - chr7.1:12640328-12640347 None:intergenic 35.0%
CATACATCTTTGACCAATGT+TGG - chr7.1:12639076-12639095 None:intergenic 35.0%
CTTCTAACAATTCTGCTTCT+AGG + chr7.1:12640471-12640490 MS.gene04858:CDS 35.0%
TCATGTTAAAAGCCATTTGC+AGG + chr7.1:12640713-12640732 MS.gene04858:CDS 35.0%
TGTAAAATTCTCTCCTCCAT+AGG - chr7.1:12639402-12639421 None:intergenic 35.0%
TGTGAATGGTGAATTGTGTT+GGG + chr7.1:12639879-12639898 MS.gene04858:intron 35.0%
TGTGTTCTGATCCAACAATT+TGG - chr7.1:12639157-12639176 None:intergenic 35.0%
TTATCTTTCAGAAAGACCTG+AGG + chr7.1:12640966-12640985 MS.gene04858:intron 35.0%
TTGTGAATGGTGAATTGTGT+TGG + chr7.1:12639878-12639897 MS.gene04858:intron 35.0%
TTGTGATATGGGGAATAAGA+AGG + chr7.1:12639906-12639925 MS.gene04858:intron 35.0%
TTTAGGCAAAATACATCGTG+CGG - chr7.1:12640222-12640241 None:intergenic 35.0%
! ATTCTTTTAACGGAGTTGGA+TGG + chr7.1:12640097-12640116 MS.gene04858:intron 35.0%
! CAAACATCTCATGATGGTTT+TGG + chr7.1:12639236-12639255 MS.gene04858:CDS 35.0%
! GTGATTCCTCAATTTGCTTA+AGG - chr7.1:12641163-12641182 None:intergenic 35.0%
!! ATGAGATTGAAGTTTCTGGT+TGG + chr7.1:12639324-12639343 MS.gene04858:CDS 35.0%
!!! CATCATGTTTTTTCGGAAAG+TGG - chr7.1:12639550-12639569 None:intergenic 35.0%
AAGAAGAGTAAGATGGACAG+AGG + chr7.1:12640511-12640530 MS.gene04858:CDS 40.0%
AAGCAGACTCCTCAACTTAA+TGG + chr7.1:12640437-12640456 MS.gene04858:CDS 40.0%
ACTCAATGGGAATCCTACAT+AGG + chr7.1:12639098-12639117 MS.gene04858:CDS 40.0%
AGCCTGACTATAGTAAGTCA+TGG - chr7.1:12640400-12640419 None:intergenic 40.0%
ATAATCGTTGATCACCTTGG+TGG + chr7.1:12640554-12640573 MS.gene04858:CDS 40.0%
ATAATGAATTGCGGAGGGTA+AGG + chr7.1:12640022-12640041 MS.gene04858:intron 40.0%
ATGCTGAATTGTCAAGCCTA+TGG + chr7.1:12639383-12639402 MS.gene04858:CDS 40.0%
ATTGGAGGGTCTGTTATCTT+TGG - chr7.1:12639365-12639384 None:intergenic 40.0%
CAAGGTCAAACATCTCATGA+TGG + chr7.1:12639230-12639249 MS.gene04858:CDS 40.0%
GAGGATGAGATTGAAGTTTC+TGG + chr7.1:12639320-12639339 MS.gene04858:CDS 40.0%
GGACAATAATGAATTGCGGA+GGG + chr7.1:12640017-12640036 MS.gene04858:intron 40.0%
GTAGTGACAATGTTGTTGCA+GGG - chr7.1:12639203-12639222 None:intergenic 40.0%
TAATGAATTGCGGAGGGTAA+GGG + chr7.1:12640023-12640042 MS.gene04858:intron 40.0%
TAGCAGCCTTAAGCAAATTG+AGG + chr7.1:12641154-12641173 MS.gene04858:intron 40.0%
TAGGCTTGACAATTCAGCAT+TGG - chr7.1:12639383-12639402 None:intergenic 40.0%
TGTAGATCGACATTTCTGCA+CGG - chr7.1:12640838-12640857 None:intergenic 40.0%
TGTAGTGACAATGTTGTTGC+AGG - chr7.1:12639204-12639223 None:intergenic 40.0%
TGTTGGGCTGATTGTGATAT+GGG + chr7.1:12639895-12639914 MS.gene04858:intron 40.0%
! AGCGCTATTACGTCGATTAA+AGG + chr7.1:12640195-12640214 MS.gene04858:intron 40.0%
! CTTGACAATTCAGCATTGGA+GGG - chr7.1:12639379-12639398 None:intergenic 40.0%
! GACGAGTTGGAGAAATTGTT+AGG - chr7.1:12640315-12640334 None:intergenic 40.0%
! GCACACATTCCATTAAGTTG+AGG - chr7.1:12640449-12640468 None:intergenic 40.0%
! GCGCTATTACGTCGATTAAA+GGG + chr7.1:12640196-12640215 MS.gene04858:intron 40.0%
! TCAATTTGCTTAAGGCTGCT+AGG - chr7.1:12641155-12641174 None:intergenic 40.0%
! TTTTGCACATGCGTGACTTT+AGG - chr7.1:12640239-12640258 None:intergenic 40.0%
AATTGCAGAGGCAAAGCCTA+AGG + chr7.1:12640650-12640669 MS.gene04858:intron 45.0%
ACGCATGCATAAAGCTTTGC+AGG + chr7.1:12640860-12640879 MS.gene04858:CDS 45.0%
ACTTACTTTCTGGACCACCA+AGG - chr7.1:12640571-12640590 None:intergenic 45.0%
CAAGGAGTTTGTGAACTGAG+AGG - chr7.1:12639426-12639445 None:intergenic 45.0%
CGCATGCATAAAGCTTTGCA+GGG + chr7.1:12640861-12640880 MS.gene04858:CDS 45.0%
CTGAATTGTCAAGCCTATGG+AGG + chr7.1:12639386-12639405 MS.gene04858:CDS 45.0%
CTGACCCAACAACCTTAACT+AGG - chr7.1:12639133-12639152 None:intergenic 45.0%
CTTTCAGAAAGACCTGAGGA+AGG + chr7.1:12640970-12640989 MS.gene04858:intron 45.0%
GAGTCAATTACCGGCAAATG+AGG - chr7.1:12640793-12640812 None:intergenic 45.0%
GCTTAAGGCTGCTAGGAAAT+AGG - chr7.1:12641148-12641167 None:intergenic 45.0%
GGGACAATAATGAATTGCGG+AGG + chr7.1:12640016-12640035 MS.gene04858:intron 45.0%
GTGTTGGGCTGATTGTGATA+TGG + chr7.1:12639894-12639913 MS.gene04858:intron 45.0%
GTTGGGCTGATTGTGATATG+GGG + chr7.1:12639896-12639915 MS.gene04858:intron 45.0%
GTTGGGTCAGACCAAATTGT+TGG + chr7.1:12639143-12639162 MS.gene04858:CDS 45.0%
TCTGTTCTATGTCCTTCCTC+AGG - chr7.1:12640985-12641004 None:intergenic 45.0%
TTCAAGGGGCTTCTCATCAA+TGG - chr7.1:12639594-12639613 None:intergenic 45.0%
TTCTGTGAAGCCTCATTTGC+CGG + chr7.1:12640780-12640799 MS.gene04858:intron 45.0%
TTGTTGCAGGGTGACTTGAT+TGG - chr7.1:12639191-12639210 None:intergenic 45.0%
! GCTTGACAATTCAGCATTGG+AGG - chr7.1:12639380-12639399 None:intergenic 45.0%
ATCACGGCAGTTGCATCTAG+AGG + chr7.1:12641178-12641197 MS.gene04858:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 12639068 12641229 12639068 ID=MS.gene04858
chr7.1 mRNA 12639068 12641229 12639068 ID=MS.gene04858.t1;Parent=MS.gene04858
chr7.1 exon 12639068 12639634 12639068 ID=MS.gene04858.t1.exon1;Parent=MS.gene04858.t1
chr7.1 CDS 12639068 12639634 12639068 ID=cds.MS.gene04858.t1;Parent=MS.gene04858.t1
chr7.1 exon 12640413 12640584 12640413 ID=MS.gene04858.t1.exon2;Parent=MS.gene04858.t1
chr7.1 CDS 12640413 12640584 12640413 ID=cds.MS.gene04858.t1;Parent=MS.gene04858.t1
chr7.1 exon 12640658 12640734 12640658 ID=MS.gene04858.t1.exon3;Parent=MS.gene04858.t1
chr7.1 CDS 12640658 12640734 12640658 ID=cds.MS.gene04858.t1;Parent=MS.gene04858.t1
chr7.1 exon 12640843 12640882 12640843 ID=MS.gene04858.t1.exon4;Parent=MS.gene04858.t1
chr7.1 CDS 12640843 12640882 12640843 ID=cds.MS.gene04858.t1;Parent=MS.gene04858.t1
chr7.1 exon 12640977 12641031 12640977 ID=MS.gene04858.t1.exon5;Parent=MS.gene04858.t1
chr7.1 CDS 12640977 12641031 12640977 ID=cds.MS.gene04858.t1;Parent=MS.gene04858.t1
chr7.1 exon 12641160 12641229 12641160 ID=MS.gene04858.t1.exon6;Parent=MS.gene04858.t1
chr7.1 CDS 12641160 12641229 12641160 ID=cds.MS.gene04858.t1;Parent=MS.gene04858.t1
Gene Sequence

>MS.gene04858

ATGTACCAACATTGGTCAAAGATGTATGGAACTCAATGGGAATCCTACATAGGAATTAACAACCTAGTTAAGGTTGTTGGGTCAGACCAAATTGTTGGATCAGAACACATGAGTTATGAGCCAATCAAGTCACCCTGCAACAACATTGTCACTACAAATTTTCAAGGTCAAACATCTCATGATGGTTTTGGTGCAGTGAGTGTAGCCAAAATCAAAAGTAATGTTAGTTTTCAAGGTGAAGAATATTATGATGAGGATGAGATTGAAGTTTCTGGTTGGTGTTTTGATCAGTTTCCAAAGATAACAGACCCTCCAATGCTGAATTGTCAAGCCTATGGAGGAGAGAATTTTACAACCTCTCAGTTCACAAACTCCTTGTACTCTGTTGATGAATCTTTGTTGTCTAACAGTGCAGATAGTCATAATTCTTCTGAAGAAGAATATAGTAATTTTCAGTCTGGAAATATGTCATTTTATGACCACTTTCCGAAAAAACATGATGAATTGTTTAGGAATGATGCTTCCATTGATGAGAAGCCCCTTGAAATTTCTTTTCAGAGAACTAAGGTAATTCTTTCTTTCTTTCTTTCTTTGTATGTAGCTTGTAATTTTTTATTCCATCTGAATTATATATGAAATGATATATCATGTTGTGCTTTAACTTGTTTAATTATATTAGAGATTAGTGCTGTCCAAAAAAAGTTACAGTATTCTTTGTGCATTCATAACCTGTTTAATTATAGTATTAGATAATAGATATTAGTCCTTTTAAGTATTTCATGGATTTTTTTTTTTAATGGTTTCATCTATTTGTGAATGGTGAATTGTGTTGGGCTGATTGTGATATGGGGAATAAGAAGGTGATATAATATGAGATTGAAATTAATGTTTTGATTTTTCTTCCATATATGCAATGAATCTAATTATGAAATTTAGGGTTTTGTGAAAGGGACAATAATGAATTGCGGAGGGTAAGGGTCCAATCTAACGTGTTTTTCTAATAAAATTGATTAAAGTGGTCACATAAACATTCTTTTAACGGAGTTGGATGGAAAACTAAAAAGAAAATCAATGGTGTCCAAGTTATGGGACTTATAGTTGGGAATTTTTTTTTAAAGGACTACTAAAGCGCTATTACGTCGATTAAAGGGCCGCACGATGTATTTTGCCTAAAGTCACGCATGTGCAAAAACAAGAGTAGTTTACTAGTAATCTATTTATTCGGGTTAAGTGCTAAGTGTTAACCTAACAATTTCTCCAACTCGTCAATTAGGATATTGTAATCTATGCCTTTTTATTTGTTTATTTTCCTTTCTAATAAGCTATAATCCATGACTTACTATAGTCAGGCTCATCTACAAAGTCACAAAAGCAGACTCCTCAACTTAATGGAATGTGTGCCACTTCTAACAATTCTGCTTCTAGGAAAGCTCCAACATGTAAAAGAAGAGTAAGATGGACAGAGGATCTGCATGAATCATTCATGATAATCGTTGATCACCTTGGTGGTCCAGAAAGTAAGTAATTAACTAAGTAATAGCATTAGTTACACATAAGCAAATTCAATTAATTCACTGATTATAATTGCAGAGGCAAAGCCTAAGGCTATACTAGATATGATGAAATCAAATTTGCTATCCATTTCTCATGTTAAAAGCCATTTGCAGGTAAAACTATTTCCATTCAACAATTTCAATAATTTGTAAATGTTGTTCTGTGAAGCCTCATTTGCCGGTAATTGACTCTAACATTGTTCTTATATGATTGCCGTGCAGAAATGTCGATCTACAATACGCATGCATAAAGCTTTGCAGGGTAAATTAATTTTTTCATTGCATTCTATTACAACACTGCTTACTTATTAAGGAGGCTATGCGTATATTGTGACACGTTTATCTTTATCTTTCAGAAAGACCTGAGGAAGGACATAGAACAGATAGAGTCGCAGATCTTCAACTCAAAATGTAAATATTTTAGTGTTACGAATGATACTTATTGATTCTTTATGTAGTAATTGCAAGTTTTTTTTTTTTTTCTCTTCTGATTACTGGTTTTTTAAATTTATACACTTTGTTTTCCTATTTCCTAGCAGCCTTAAGCAAATTGAGGAATCACGGCAGTTGCATCTAGAGGTTCGAAAAAGTATTTCGCAACAGTTAGAG
Protein sequence

>MS.gene04858.t1

MYQHWSKMYGTQWESYIGINNLVKVVGSDQIVGSEHMSYEPIKSPCNNIVTTNFQGQTSHDGFGAVSVAKIKSNVSFQGEEYYDEDEIEVSGWCFDQFPKITDPPMLNCQAYGGENFTTSQFTNSLYSVDESLLSNSADSHNSSEEEYSNFQSGNMSFYDHFPKKHDELFRNDASIDEKPLEISFQRTKSGSSTKSQKQTPQLNGMCATSNNSASRKAPTCKRRVRWTEDLHESFMIIVDHLGGPEKAKPKAILDMMKSNLLSISHVKSHLQKCRSTIRMHKALQERPEEGHRTDRVADLQLKILKQIEESRQLHLEVRKSISQQLE