Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048630.t1 | XP_013449754.1 | 71.8 | 216 | 53 | 3 | 1 | 215 | 1 | 209 | 3.80E-65 | 258.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048630.t1 | Q9SKY9 | 54.4 | 68 | 28 | 1 | 144 | 208 | 171 | 238 | 6.4e-13 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048630.t1 | A0A072U262 | 71.8 | 216 | 53 | 3 | 1 | 215 | 1 | 209 | 2.7e-65 | 258.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene048630.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048630.t1 | MTR_7g095150 | 84.135 | 208 | 25 | 3 | 1 | 207 | 1 | 201 | 1.44e-112 | 321 |
| MS.gene048630.t1 | MTR_1g072110 | 50.427 | 234 | 78 | 7 | 12 | 209 | 6 | 237 | 1.12e-61 | 192 |
| MS.gene048630.t1 | MTR_1g103640 | 46.617 | 133 | 51 | 4 | 75 | 206 | 66 | 179 | 6.24e-30 | 111 |
| MS.gene048630.t1 | MTR_2g102140 | 36.691 | 139 | 75 | 2 | 88 | 213 | 142 | 280 | 2.52e-19 | 84.7 |
| MS.gene048630.t1 | MTR_7g105780 | 35.032 | 157 | 79 | 5 | 50 | 206 | 66 | 199 | 1.55e-18 | 82.0 |
| MS.gene048630.t1 | MTR_4g012060 | 49.275 | 69 | 33 | 1 | 135 | 203 | 183 | 249 | 1.35e-14 | 71.2 |
| MS.gene048630.t1 | MTR_1g111640 | 48.529 | 68 | 29 | 2 | 137 | 204 | 138 | 199 | 1.45e-13 | 68.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048630.t1 | AT2G32100 | 37.313 | 201 | 80 | 7 | 49 | 208 | 43 | 238 | 3.27e-28 | 107 |
| MS.gene048630.t1 | AT1G05420 | 42.857 | 133 | 55 | 4 | 78 | 208 | 107 | 220 | 3.61e-25 | 98.6 |
| MS.gene048630.t1 | AT1G05420 | 42.857 | 133 | 55 | 4 | 78 | 208 | 123 | 236 | 3.85e-25 | 99.0 |
| MS.gene048630.t1 | AT4G14860 | 50.000 | 78 | 36 | 2 | 133 | 208 | 96 | 172 | 9.42e-20 | 83.2 |
| MS.gene048630.t1 | AT5G04820 | 35.616 | 146 | 80 | 5 | 63 | 206 | 79 | 212 | 9.24e-18 | 79.7 |
| MS.gene048630.t1 | AT1G79960 | 41.026 | 117 | 49 | 4 | 90 | 206 | 164 | 260 | 6.32e-17 | 77.8 |
| MS.gene048630.t1 | AT2G36050 | 31.452 | 124 | 58 | 2 | 83 | 206 | 77 | 173 | 6.34e-14 | 69.3 |
Find 58 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGGTTCGGGTTTGGAATC+TGG | 0.244871 | 7.4:-14127827 | None:intergenic |
| ATGTCGGCGGGTTGAGGTTC+GGG | 0.249268 | 7.4:-14127839 | None:intergenic |
| AATAAACTTGTGAGTGTTCT+TGG | 0.269260 | 7.4:-14128191 | None:intergenic |
| AATGTCGGCGGGTTGAGGTT+CGG | 0.312904 | 7.4:-14127840 | None:intergenic |
| CTTCTTGTTCTAAGTGTAGT+TGG | 0.316838 | 7.4:-14127632 | None:intergenic |
| AGAAGATGAGGAGGAGGAGG+AGG | 0.357794 | 7.4:-14127942 | None:intergenic |
| GGCGGGTTGAGGTTCGGGTT+TGG | 0.367644 | 7.4:-14127834 | None:intergenic |
| TGTGATCAATTTGGTGATGT+TGG | 0.393032 | 7.4:-14127730 | None:intergenic |
| ATGGAGGATTGTGATCAATT+TGG | 0.401416 | 7.4:-14127739 | None:intergenic |
| GGAAGAAGATGAGGAGGAGG+AGG | 0.404203 | 7.4:-14127945 | None:intergenic |
| TGAAAGACGTGAAGTCCAAC+TGG | 0.409296 | 7.4:+14128125 | MS.gene048630:CDS |
| TTAGAATCATTTGTCGGTGC+TGG | 0.428480 | 7.4:-14127989 | None:intergenic |
| CTCATCTTCTTCCTCCTCGT+TGG | 0.428907 | 7.4:+14127955 | MS.gene048630:CDS |
| GACTGCCGAAAATCCATGTA+AGG | 0.436850 | 7.4:-14128067 | None:intergenic |
| ATAAACTTGTGAGTGTTCTT+GGG | 0.441701 | 7.4:-14128190 | None:intergenic |
| GTGGCAATGTCGGCGGGTTG+AGG | 0.442171 | 7.4:-14127845 | None:intergenic |
| GGAGGAAGAAGATGAGGAGG+AGG | 0.444956 | 7.4:-14127948 | None:intergenic |
| ATAGGTAATTGAGGTGTTTG+AGG | 0.446472 | 7.4:-14127664 | None:intergenic |
| ATACTCACCGGACCCTTACA+TGG | 0.446488 | 7.4:+14128054 | MS.gene048630:CDS |
| GGATGAGGTGGATTCAACTA+TGG | 0.452726 | 7.4:-14127918 | None:intergenic |
| TGTTCTAAGTGTAGTTGGCA+TGG | 0.478635 | 7.4:-14127627 | None:intergenic |
| TGTTGAAGAGGAGGAGATTG+AGG | 0.483301 | 7.4:-14127707 | None:intergenic |
| TTCACGTCTTTCATCAACTC+TGG | 0.488171 | 7.4:-14128115 | None:intergenic |
| GGGTTTGGAATCTGGTTCGA+TGG | 0.501378 | 7.4:-14127819 | None:intergenic |
| TTTGATCATGGTTGATGACT+TGG | 0.504706 | 7.4:-14127768 | None:intergenic |
| TTGAGAAACATAGGTAATTG+AGG | 0.507591 | 7.4:-14127673 | None:intergenic |
| GCTAAGAAGAAGCTCGTGTA+AGG | 0.508873 | 7.4:-14128152 | None:intergenic |
| CGAAAATCCATGTAAGGGTC+CGG | 0.511393 | 7.4:-14128061 | None:intergenic |
| GGCGATAGCGGTGGCAATGT+CGG | 0.513648 | 7.4:-14127855 | None:intergenic |
| ATAGCGGTGGCAATGTCGGC+GGG | 0.519957 | 7.4:-14127851 | None:intergenic |
| TGGAATCTGGTTCGATGGAG+TGG | 0.523377 | 7.4:-14127814 | None:intergenic |
| GGTGGATTCAACTATGGAGT+TGG | 0.526250 | 7.4:-14127912 | None:intergenic |
| GTATGAAAGTTGAGAAACAT+AGG | 0.539754 | 7.4:-14127682 | None:intergenic |
| CGAGGAGGAAGAAGATGAGG+AGG | 0.566205 | 7.4:-14127951 | None:intergenic |
| CTCGTGTAAGGTATCCCAGT+TGG | 0.568383 | 7.4:-14128140 | None:intergenic |
| GGCGGTGCCAACATACTCAC+CGG | 0.568623 | 7.4:+14128042 | MS.gene048630:CDS |
| ATCATTTGTCGGTGCTGGCA+CGG | 0.571546 | 7.4:-14127984 | None:intergenic |
| TGGTGATGTTGGTGTTGAAG+AGG | 0.571921 | 7.4:-14127719 | None:intergenic |
| CTGGTTCGATGGAGTGGTGG+TGG | 0.576085 | 7.4:-14127808 | None:intergenic |
| GATAGCGGTGGCAATGTCGG+CGG | 0.576430 | 7.4:-14127852 | None:intergenic |
| GATGACTTGGAGAGTGATGG+AGG | 0.578578 | 7.4:-14127755 | None:intergenic |
| CAACGAGGAGGAAGAAGATG+AGG | 0.579217 | 7.4:-14127954 | None:intergenic |
| ATACACTATCACGAAGAAGA+TGG | 0.579944 | 7.4:-14128274 | None:intergenic |
| TCGGCAGTCAATGCAAGAGA+TGG | 0.582966 | 7.4:+14128081 | MS.gene048630:CDS |
| GCAGTCAATGCAAGAGATGG+TGG | 0.593765 | 7.4:+14128084 | MS.gene048630:CDS |
| GAAGCGGTTGGAGGCGATAG+CGG | 0.600134 | 7.4:-14127867 | None:intergenic |
| TAAGGGTCCGGTGAGTATGT+TGG | 0.613914 | 7.4:-14128049 | None:intergenic |
| GTTGATGACTTGGAGAGTGA+TGG | 0.615673 | 7.4:-14127758 | None:intergenic |
| TGATGTTGGTGTTGAAGAGG+AGG | 0.624026 | 7.4:-14127716 | None:intergenic |
| GCGGTTGGAGGCGATAGCGG+TGG | 0.625107 | 7.4:-14127864 | None:intergenic |
| AATCTGGTTCGATGGAGTGG+TGG | 0.632867 | 7.4:-14127811 | None:intergenic |
| ACTGCCGAAAATCCATGTAA+GGG | 0.646543 | 7.4:-14128066 | None:intergenic |
| ACTATGGAGTTGGAACGCCC+CGG | 0.655856 | 7.4:-14127902 | None:intergenic |
| GGAGTTGGAACGCCCCGGAG+AGG | 0.679932 | 7.4:-14127897 | None:intergenic |
| GAAAGACGTGAAGTCCAACT+GGG | 0.680650 | 7.4:+14128126 | MS.gene048630:CDS |
| CACGGTGGAAACCAACGAGG+AGG | 0.688972 | 7.4:-14127966 | None:intergenic |
| ATTTGTCGGTGCTGGCACGG+TGG | 0.715454 | 7.4:-14127981 | None:intergenic |
| TGGCACGGTGGAAACCAACG+AGG | 0.765920 | 7.4:-14127969 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTGTTAGAATCATTTGT+CGG | - | chr7.4:14127998-14128017 | None:intergenic | 20.0% |
| !!! | AAAAAGAAGACAGTTTTGAA+CGG | + | chr7.4:14128013-14128032 | MS.gene048630:CDS | 25.0% |
| AATAAACTTGTGAGTGTTCT+TGG | - | chr7.4:14128194-14128213 | None:intergenic | 30.0% | |
| ATAAACTTGTGAGTGTTCTT+GGG | - | chr7.4:14128193-14128212 | None:intergenic | 30.0% | |
| GTATGAAAGTTGAGAAACAT+AGG | - | chr7.4:14127685-14127704 | None:intergenic | 30.0% | |
| TTGAGAAACATAGGTAATTG+AGG | - | chr7.4:14127676-14127695 | None:intergenic | 30.0% | |
| !! | AGTGTTGAAGTTTTTGATCA+TGG | - | chr7.4:14127783-14127802 | None:intergenic | 30.0% |
| ATGGAGGATTGTGATCAATT+TGG | - | chr7.4:14127742-14127761 | None:intergenic | 35.0% | |
| ! | ATAGGTAATTGAGGTGTTTG+AGG | - | chr7.4:14127667-14127686 | None:intergenic | 35.0% |
| ! | CTTCTTGTTCTAAGTGTAGT+TGG | - | chr7.4:14127635-14127654 | None:intergenic | 35.0% |
| !! | TGTGATCAATTTGGTGATGT+TGG | - | chr7.4:14127733-14127752 | None:intergenic | 35.0% |
| !! | TTTGATCATGGTTGATGACT+TGG | - | chr7.4:14127771-14127790 | None:intergenic | 35.0% |
| ACTGCCGAAAATCCATGTAA+GGG | - | chr7.4:14128069-14128088 | None:intergenic | 40.0% | |
| TTAGAATCATTTGTCGGTGC+TGG | - | chr7.4:14127992-14128011 | None:intergenic | 40.0% | |
| TTCACGTCTTTCATCAACTC+TGG | - | chr7.4:14128118-14128137 | None:intergenic | 40.0% | |
| AGAGGAGAAAAAGAAGCGGT+TGG | - | chr7.4:14127882-14127901 | None:intergenic | 45.0% | |
| CGAAAATCCATGTAAGGGTC+CGG | - | chr7.4:14128064-14128083 | None:intergenic | 45.0% | |
| GAAAGACGTGAAGTCCAACT+GGG | + | chr7.4:14128126-14128145 | MS.gene048630:CDS | 45.0% | |
| GACTGCCGAAAATCCATGTA+AGG | - | chr7.4:14128070-14128089 | None:intergenic | 45.0% | |
| GCTAAGAAGAAGCTCGTGTA+AGG | - | chr7.4:14128155-14128174 | None:intergenic | 45.0% | |
| TGAAAGACGTGAAGTCCAAC+TGG | + | chr7.4:14128125-14128144 | MS.gene048630:CDS | 45.0% | |
| TGTTGAAGAGGAGGAGATTG+AGG | - | chr7.4:14127710-14127729 | None:intergenic | 45.0% | |
| ! | CGGACCCTTACATGGATTTT+CGG | + | chr7.4:14128062-14128081 | MS.gene048630:CDS | 45.0% |
| ! | GGATGAGGTGGATTCAACTA+TGG | - | chr7.4:14127921-14127940 | None:intergenic | 45.0% |
| ! | GGTGGATTCAACTATGGAGT+TGG | - | chr7.4:14127915-14127934 | None:intergenic | 45.0% |
| !! | GTTGATGACTTGGAGAGTGA+TGG | - | chr7.4:14127761-14127780 | None:intergenic | 45.0% |
| !! | TGATGTTGGTGTTGAAGAGG+AGG | - | chr7.4:14127719-14127738 | None:intergenic | 45.0% |
| !! | TGGTGATGTTGGTGTTGAAG+AGG | - | chr7.4:14127722-14127741 | None:intergenic | 45.0% |
| !!! | AGTTTTGAACGGAAGTGTGG+CGG | + | chr7.4:14128024-14128043 | MS.gene048630:CDS | 45.0% |
| !!! | GACAGTTTTGAACGGAAGTG+TGG | + | chr7.4:14128021-14128040 | MS.gene048630:CDS | 45.0% |
| ATACTCACCGGACCCTTACA+TGG | + | chr7.4:14128054-14128073 | MS.gene048630:CDS | 50.0% | |
| CAACGAGGAGGAAGAAGATG+AGG | - | chr7.4:14127957-14127976 | None:intergenic | 50.0% | |
| CCGGAGAGGAGAAAAAGAAG+CGG | - | chr7.4:14127886-14127905 | None:intergenic | 50.0% | |
| CTCATCTTCTTCCTCCTCGT+TGG | + | chr7.4:14127955-14127974 | MS.gene048630:CDS | 50.0% | |
| GCAGTCAATGCAAGAGATGG+TGG | + | chr7.4:14128084-14128103 | MS.gene048630:CDS | 50.0% | |
| GGAGAAAAAGAAGCGGTTGG+AGG | - | chr7.4:14127879-14127898 | None:intergenic | 50.0% | |
| TAAGGGTCCGGTGAGTATGT+TGG | - | chr7.4:14128052-14128071 | None:intergenic | 50.0% | |
| TCGGCAGTCAATGCAAGAGA+TGG | + | chr7.4:14128081-14128100 | MS.gene048630:CDS | 50.0% | |
| ! | AATCTGGTTCGATGGAGTGG+TGG | - | chr7.4:14127814-14127833 | None:intergenic | 50.0% |
| ! | CCGCTTCTTTTTCTCCTCTC+CGG | + | chr7.4:14127883-14127902 | MS.gene048630:CDS | 50.0% |
| ! | CGCTTCTTTTTCTCCTCTCC+GGG | + | chr7.4:14127884-14127903 | MS.gene048630:CDS | 50.0% |
| ! | CTCGTGTAAGGTATCCCAGT+TGG | - | chr7.4:14128143-14128162 | None:intergenic | 50.0% |
| ! | GATGACTTGGAGAGTGATGG+AGG | - | chr7.4:14127758-14127777 | None:intergenic | 50.0% |
| ! | GCTTCTTTTTCTCCTCTCCG+GGG | + | chr7.4:14127885-14127904 | MS.gene048630:CDS | 50.0% |
| ! | GGGTTTGGAATCTGGTTCGA+TGG | - | chr7.4:14127822-14127841 | None:intergenic | 50.0% |
| ! | TGAGGTTCGGGTTTGGAATC+TGG | - | chr7.4:14127830-14127849 | None:intergenic | 50.0% |
| ! | TGGAATCTGGTTCGATGGAG+TGG | - | chr7.4:14127817-14127836 | None:intergenic | 50.0% |
| !! | ATCATTTGTCGGTGCTGGCA+CGG | - | chr7.4:14127987-14128006 | None:intergenic | 50.0% |
| ACTATGGAGTTGGAACGCCC+CGG | - | chr7.4:14127905-14127924 | None:intergenic | 55.0% | |
| AGAAGATGAGGAGGAGGAGG+AGG | - | chr7.4:14127945-14127964 | None:intergenic | 55.0% | |
| CGAGGAGGAAGAAGATGAGG+AGG | - | chr7.4:14127954-14127973 | None:intergenic | 55.0% | |
| GGAAGAAGATGAGGAGGAGG+AGG | - | chr7.4:14127948-14127967 | None:intergenic | 55.0% | |
| GGAGGAAGAAGATGAGGAGG+AGG | - | chr7.4:14127951-14127970 | None:intergenic | 55.0% | |
| !! | AATGTCGGCGGGTTGAGGTT+CGG | - | chr7.4:14127843-14127862 | None:intergenic | 55.0% |
| AGATGAGGAGGAGGAGGAGG+AGG | - | chr7.4:14127942-14127961 | None:intergenic | 60.0% | |
| ATAGCGGTGGCAATGTCGGC+GGG | - | chr7.4:14127854-14127873 | None:intergenic | 60.0% | |
| CACGGTGGAAACCAACGAGG+AGG | - | chr7.4:14127969-14127988 | None:intergenic | 60.0% | |
| GAAGCGGTTGGAGGCGATAG+CGG | - | chr7.4:14127870-14127889 | None:intergenic | 60.0% | |
| TGGCACGGTGGAAACCAACG+AGG | - | chr7.4:14127972-14127991 | None:intergenic | 60.0% | |
| ! | CTGGTTCGATGGAGTGGTGG+TGG | - | chr7.4:14127811-14127830 | None:intergenic | 60.0% |
| ! | GATAGCGGTGGCAATGTCGG+CGG | - | chr7.4:14127855-14127874 | None:intergenic | 60.0% |
| ! | GGCGATAGCGGTGGCAATGT+CGG | - | chr7.4:14127858-14127877 | None:intergenic | 60.0% |
| ! | GGCGGTGCCAACATACTCAC+CGG | + | chr7.4:14128042-14128061 | MS.gene048630:CDS | 60.0% |
| !! | ATGTCGGCGGGTTGAGGTTC+GGG | - | chr7.4:14127842-14127861 | None:intergenic | 60.0% |
| !! | ATTTGTCGGTGCTGGCACGG+TGG | - | chr7.4:14127984-14128003 | None:intergenic | 60.0% |
| GGAGGAGGAGGAGGAGGATG+AGG | - | chr7.4:14127936-14127955 | None:intergenic | 65.0% | |
| GGAGGAGGAGGAGGATGAGG+TGG | - | chr7.4:14127933-14127952 | None:intergenic | 65.0% | |
| !! | GGCGGGTTGAGGTTCGGGTT+TGG | - | chr7.4:14127837-14127856 | None:intergenic | 65.0% |
| !! | GTGGCAATGTCGGCGGGTTG+AGG | - | chr7.4:14127848-14127867 | None:intergenic | 65.0% |
| GGAGTTGGAACGCCCCGGAG+AGG | - | chr7.4:14127900-14127919 | None:intergenic | 70.0% | |
| ! | GCGGTTGGAGGCGATAGCGG+TGG | - | chr7.4:14127867-14127886 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 14127629 | 14128288 | 14127629 | ID=MS.gene048630 |
| chr7.4 | mRNA | 14127629 | 14128288 | 14127629 | ID=MS.gene048630.t1;Parent=MS.gene048630 |
| chr7.4 | exon | 14127629 | 14128288 | 14127629 | ID=MS.gene048630.t1.exon1;Parent=MS.gene048630.t1 |
| chr7.4 | CDS | 14127629 | 14128288 | 14127629 | ID=cds.MS.gene048630.t1;Parent=MS.gene048630.t1 |
| Gene Sequence |
| Protein sequence |
