Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048676.t1 | XP_003600679.1 | 91.6 | 239 | 19 | 1 | 1 | 239 | 1 | 238 | 1.40E-102 | 382.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048676.t1 | Q39263 | 50.0 | 184 | 57 | 8 | 86 | 239 | 82 | 260 | 7.2e-34 | 145.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048676.t1 | G7J8Q3 | 91.6 | 239 | 19 | 1 | 1 | 239 | 1 | 238 | 1.0e-102 | 382.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene048676.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048676.t1 | MTR_3g065020 | 93.724 | 239 | 14 | 1 | 1 | 239 | 1 | 238 | 4.26e-160 | 443 |
| MS.gene048676.t1 | MTR_5g077370 | 61.538 | 247 | 77 | 9 | 1 | 239 | 1 | 237 | 8.75e-86 | 254 |
| MS.gene048676.t1 | MTR_1g033340 | 43.426 | 251 | 124 | 7 | 3 | 239 | 5 | 251 | 3.56e-60 | 190 |
| MS.gene048676.t1 | MTR_2g104900 | 65.432 | 81 | 20 | 3 | 81 | 154 | 76 | 155 | 7.00e-27 | 104 |
| MS.gene048676.t1 | MTR_1g013130 | 52.000 | 100 | 30 | 3 | 74 | 155 | 80 | 179 | 1.53e-25 | 101 |
| MS.gene048676.t1 | MTR_1g107055 | 40.299 | 134 | 74 | 4 | 70 | 202 | 47 | 175 | 2.72e-24 | 96.3 |
| MS.gene048676.t1 | MTR_3g106230 | 49.541 | 109 | 30 | 6 | 72 | 159 | 60 | 164 | 3.29e-20 | 86.3 |
| MS.gene048676.t1 | MTR_4g006575 | 62.857 | 70 | 18 | 3 | 87 | 155 | 67 | 129 | 1.33e-18 | 81.6 |
| MS.gene048676.t1 | MTR_4g006600 | 62.857 | 70 | 18 | 3 | 87 | 155 | 67 | 129 | 1.33e-18 | 81.6 |
| MS.gene048676.t1 | MTR_4g128340 | 66.071 | 56 | 18 | 1 | 86 | 140 | 44 | 99 | 3.84e-17 | 76.6 |
| MS.gene048676.t1 | MTR_8g466760 | 48.780 | 82 | 32 | 4 | 87 | 160 | 98 | 177 | 9.72e-14 | 69.3 |
| MS.gene048676.t1 | MTR_7g083210 | 75.758 | 33 | 8 | 0 | 87 | 119 | 39 | 71 | 2.36e-12 | 63.9 |
| MS.gene048676.t1 | MTR_7g086860 | 67.568 | 37 | 12 | 0 | 87 | 123 | 30 | 66 | 5.28e-12 | 62.4 |
| MS.gene048676.t1 | MTR_5g008280 | 44.186 | 86 | 38 | 4 | 87 | 165 | 46 | 128 | 2.02e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048676.t1 | AT1G66140 | 50.000 | 184 | 57 | 8 | 86 | 239 | 82 | 260 | 1.25e-46 | 155 |
| MS.gene048676.t1 | AT1G24625 | 40.865 | 208 | 81 | 12 | 46 | 239 | 30 | 209 | 2.80e-31 | 114 |
| MS.gene048676.t1 | AT1G80730 | 53.261 | 92 | 25 | 2 | 82 | 155 | 61 | 152 | 1.09e-23 | 95.5 |
| MS.gene048676.t1 | AT5G10970 | 45.902 | 122 | 50 | 5 | 47 | 155 | 56 | 174 | 1.76e-22 | 93.2 |
| MS.gene048676.t1 | AT5G25160 | 56.410 | 78 | 28 | 2 | 84 | 155 | 56 | 133 | 7.49e-22 | 90.9 |
| MS.gene048676.t1 | AT5G57520 | 52.113 | 71 | 34 | 0 | 87 | 157 | 50 | 120 | 3.56e-16 | 73.6 |
| MS.gene048676.t1 | AT5G14010 | 66.667 | 39 | 13 | 0 | 86 | 124 | 35 | 73 | 1.19e-13 | 67.0 |
| MS.gene048676.t1 | AT3G58070 | 55.357 | 56 | 21 | 1 | 67 | 122 | 73 | 124 | 8.02e-12 | 63.5 |
| MS.gene048676.t1 | AT5G01860 | 48.214 | 56 | 29 | 0 | 71 | 126 | 49 | 104 | 1.16e-11 | 62.8 |
Find 41 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCTCTTAATGGTTCTTTC+AGG | 0.209061 | 3.4:+66556202 | MS.gene048676:CDS |
| CTTGCATCTCTGCCTCTTAA+TGG | 0.234261 | 3.4:+66556192 | MS.gene048676:CDS |
| CAAAGTTTCAATGTTAATTC+AGG | 0.241054 | 3.4:-66556489 | None:intergenic |
| AATGGTTCTTTCAGGTCATT+AGG | 0.297972 | 3.4:+66556210 | MS.gene048676:CDS |
| AATTGCAAGAGAAAATTCTT+TGG | 0.318566 | 3.4:-66556047 | None:intergenic |
| TTTCTCTCTTGTGTGCGTTT+TGG | 0.337168 | 3.4:-66556113 | None:intergenic |
| AACCTGGAGAGGTTTCAATT+TGG | 0.372940 | 3.4:-66555882 | None:intergenic |
| GCAAAGCGAGCTATGCGAAT+TGG | 0.384062 | 3.4:+66556144 | MS.gene048676:CDS |
| TATCGGTCATCCGATATTCT+TGG | 0.398517 | 3.4:+66556329 | MS.gene048676:CDS |
| GGGCTAGGCCTTATTGTTGA+TGG | 0.404082 | 3.4:-66556270 | None:intergenic |
| ATTAGAAAGGCTGTTACTAT+AGG | 0.421832 | 3.4:-66555905 | None:intergenic |
| CTGGAGAGGTTTCAATTTGG+TGG | 0.433921 | 3.4:-66555879 | None:intergenic |
| TCATCGTCCTCCAAGAATAT+CGG | 0.434665 | 3.4:-66556339 | None:intergenic |
| AATTTGGTGGGTGGATATAG+TGG | 0.440124 | 3.4:-66555866 | None:intergenic |
| TAATTCAGATTCCATCTCAC+TGG | 0.457739 | 3.4:+66555924 | MS.gene048676:CDS |
| AAGTTCATTGCTGCTCTCAC+TGG | 0.464380 | 3.4:-66556019 | None:intergenic |
| GCAAGAGAAAATTCTTTGGA+TGG | 0.480537 | 3.4:-66556043 | None:intergenic |
| TTGGTTGCAAGAGATTCAAC+AGG | 0.483148 | 3.4:+66555979 | MS.gene048676:CDS |
| AGCAGTGCTAGATTCAAGTT+AGG | 0.490778 | 3.4:+66556303 | MS.gene048676:CDS |
| GACCTGAAAGAACCATTAAG+AGG | 0.490795 | 3.4:-66556204 | None:intergenic |
| TATAGCTCGCAGGCACTTGG+TGG | 0.518085 | 3.4:+66556087 | MS.gene048676:CDS |
| CTTCAACAAGGACAATGACT+TGG | 0.521722 | 3.4:+66555960 | MS.gene048676:CDS |
| TGGAGAGGTTTCAATTTGGT+GGG | 0.530109 | 3.4:-66555878 | None:intergenic |
| CACCAAATTGAAACCTCTCC+AGG | 0.534773 | 3.4:+66555880 | MS.gene048676:CDS |
| AGATGGAATCTGAATTAGAA+AGG | 0.546754 | 3.4:-66555918 | None:intergenic |
| AGATTCAAGTTAGGATCTAT+CGG | 0.563874 | 3.4:+66556312 | MS.gene048676:CDS |
| CGGTCATCCGATATTCTTGG+AGG | 0.568620 | 3.4:+66556332 | MS.gene048676:CDS |
| GAATTCTCTATGTCAACCAA+TGG | 0.572984 | 3.4:-66556462 | None:intergenic |
| AGAGGTTTCAATTTGGTGGG+TGG | 0.575688 | 3.4:-66555875 | None:intergenic |
| GAGTGTTAAGTCCAGTGAGA+TGG | 0.580909 | 3.4:-66555935 | None:intergenic |
| ATCAGATTCATATTCTTCTG+TGG | 0.583159 | 3.4:-66555824 | None:intergenic |
| TTTCATCAGGTTACTGAGGG+AGG | 0.597854 | 3.4:+66556387 | MS.gene048676:CDS |
| TGGATATAGTGGAAGCAACA+TGG | 0.607959 | 3.4:-66555855 | None:intergenic |
| AGGCTGTTACTATAGGAACC+TGG | 0.608616 | 3.4:-66555898 | None:intergenic |
| TGAGGTGAATGTGAAACCAT+TGG | 0.612040 | 3.4:+66556446 | MS.gene048676:CDS |
| ACGATGAGACAGAACTGATG+TGG | 0.646574 | 3.4:+66556355 | MS.gene048676:CDS |
| GAGACAGAACTGATGTGGCA+AGG | 0.647919 | 3.4:+66556360 | MS.gene048676:CDS |
| CTTAACACTCAACTTCAACA+AGG | 0.655631 | 3.4:+66555948 | MS.gene048676:CDS |
| GTTACTATAGGAACCTGGAG+AGG | 0.688967 | 3.4:-66555893 | None:intergenic |
| ACACACTCTTCACTACACCA+TGG | 0.697590 | 3.4:+66556240 | MS.gene048676:CDS |
| GAATTGGACTATTCACTGAG+AGG | 0.709982 | 3.4:+66556160 | MS.gene048676:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTGCAAGAGAAAATTCTT+TGG | - | chr3.4:66556050-66556069 | None:intergenic | 25.0% |
| ! | CAAAGTTTCAATGTTAATTC+AGG | - | chr3.4:66556492-66556511 | None:intergenic | 25.0% |
| ! | TCTACTTCAAGATCAAAAAT+TGG | - | chr3.4:66555802-66555821 | None:intergenic | 25.0% |
| !! | AATTTTTGATCTTGAAGTAG+AGG | + | chr3.4:66555801-66555820 | MS.gene048676:CDS | 25.0% |
| !! | TTCAAATATGTGTTTTACTG+AGG | + | chr3.4:66556428-66556447 | MS.gene048676:CDS | 25.0% |
| AGATGGAATCTGAATTAGAA+AGG | - | chr3.4:66555921-66555940 | None:intergenic | 30.0% | |
| ATCAGATTCATATTCTTCTG+TGG | - | chr3.4:66555827-66555846 | None:intergenic | 30.0% | |
| ATTAGAAAGGCTGTTACTAT+AGG | - | chr3.4:66555908-66555927 | None:intergenic | 30.0% | |
| ! | AGATTCAAGTTAGGATCTAT+CGG | + | chr3.4:66556312-66556331 | MS.gene048676:CDS | 30.0% |
| ! | TTTTCTCTTGCAATTACTGT+CGG | + | chr3.4:66556055-66556074 | MS.gene048676:CDS | 30.0% |
| CTTAACACTCAACTTCAACA+AGG | + | chr3.4:66555948-66555967 | MS.gene048676:CDS | 35.0% | |
| GAATTCTCTATGTCAACCAA+TGG | - | chr3.4:66556465-66556484 | None:intergenic | 35.0% | |
| GCAAGAGAAAATTCTTTGGA+TGG | - | chr3.4:66556046-66556065 | None:intergenic | 35.0% | |
| TAATTCAGATTCCATCTCAC+TGG | + | chr3.4:66555924-66555943 | MS.gene048676:CDS | 35.0% | |
| ! | AATGGTTCTTTCAGGTCATT+AGG | + | chr3.4:66556210-66556229 | MS.gene048676:CDS | 35.0% |
| ! | AGTTTTCATCAGGTTACTGA+GGG | + | chr3.4:66556384-66556403 | MS.gene048676:CDS | 35.0% |
| ! | GTTTTTCGCCATCAACAATA+AGG | + | chr3.4:66556262-66556281 | MS.gene048676:CDS | 35.0% |
| ! | TAGTTTTCATCAGGTTACTG+AGG | + | chr3.4:66556383-66556402 | MS.gene048676:CDS | 35.0% |
| !!! | TAGCACTGCTTTTTATCTCA+GGG | - | chr3.4:66556293-66556312 | None:intergenic | 35.0% |
| AACCTGGAGAGGTTTCAATT+TGG | - | chr3.4:66555885-66555904 | None:intergenic | 40.0% | |
| CTTCAACAAGGACAATGACT+TGG | + | chr3.4:66555960-66555979 | MS.gene048676:CDS | 40.0% | |
| GAATTGGACTATTCACTGAG+AGG | + | chr3.4:66556160-66556179 | MS.gene048676:CDS | 40.0% | |
| GACCTGAAAGAACCATTAAG+AGG | - | chr3.4:66556207-66556226 | None:intergenic | 40.0% | |
| TATCGGTCATCCGATATTCT+TGG | + | chr3.4:66556329-66556348 | MS.gene048676:CDS | 40.0% | |
| TCATCGTCCTCCAAGAATAT+CGG | - | chr3.4:66556342-66556361 | None:intergenic | 40.0% | |
| TGAGGTGAATGTGAAACCAT+TGG | + | chr3.4:66556446-66556465 | MS.gene048676:CDS | 40.0% | |
| TGCCTCTTAATGGTTCTTTC+AGG | + | chr3.4:66556202-66556221 | MS.gene048676:CDS | 40.0% | |
| TGGAGAGGTTTCAATTTGGT+GGG | - | chr3.4:66555881-66555900 | None:intergenic | 40.0% | |
| TTGGTTGCAAGAGATTCAAC+AGG | + | chr3.4:66555979-66555998 | MS.gene048676:CDS | 40.0% | |
| ! | AATTTGGTGGGTGGATATAG+TGG | - | chr3.4:66555869-66555888 | None:intergenic | 40.0% |
| ! | AGCAGTGCTAGATTCAAGTT+AGG | + | chr3.4:66556303-66556322 | MS.gene048676:CDS | 40.0% |
| ! | TGGATATAGTGGAAGCAACA+TGG | - | chr3.4:66555858-66555877 | None:intergenic | 40.0% |
| ! | TTTCTCTCTTGTGTGCGTTT+TGG | - | chr3.4:66556116-66556135 | None:intergenic | 40.0% |
| !! | TTGTTGATGGCGAAAAACCA+TGG | - | chr3.4:66556260-66556279 | None:intergenic | 40.0% |
| !!! | CTAGCACTGCTTTTTATCTC+AGG | - | chr3.4:66556294-66556313 | None:intergenic | 40.0% |
| AAGTTCATTGCTGCTCTCAC+TGG | - | chr3.4:66556022-66556041 | None:intergenic | 45.0% | |
| ACACACTCTTCACTACACCA+TGG | + | chr3.4:66556240-66556259 | MS.gene048676:CDS | 45.0% | |
| ACGATGAGACAGAACTGATG+TGG | + | chr3.4:66556355-66556374 | MS.gene048676:CDS | 45.0% | |
| AGGCTGTTACTATAGGAACC+TGG | - | chr3.4:66555901-66555920 | None:intergenic | 45.0% | |
| CACCAAATTGAAACCTCTCC+AGG | + | chr3.4:66555880-66555899 | MS.gene048676:CDS | 45.0% | |
| CTGGAGAGGTTTCAATTTGG+TGG | - | chr3.4:66555882-66555901 | None:intergenic | 45.0% | |
| CTTGCATCTCTGCCTCTTAA+TGG | + | chr3.4:66556192-66556211 | MS.gene048676:CDS | 45.0% | |
| GAGTGTTAAGTCCAGTGAGA+TGG | - | chr3.4:66555938-66555957 | None:intergenic | 45.0% | |
| GTTACTATAGGAACCTGGAG+AGG | - | chr3.4:66555896-66555915 | None:intergenic | 45.0% | |
| TTTCATCAGGTTACTGAGGG+AGG | + | chr3.4:66556387-66556406 | MS.gene048676:CDS | 45.0% | |
| ! | AGAGGTTTCAATTTGGTGGG+TGG | - | chr3.4:66555878-66555897 | None:intergenic | 45.0% |
| ! | GCGCAAATTTTATAGCTCGC+AGG | + | chr3.4:66556077-66556096 | MS.gene048676:CDS | 45.0% |
| ! | GTGGCAAGGTAGTTTTCATC+AGG | + | chr3.4:66556374-66556393 | MS.gene048676:CDS | 45.0% |
| ! | TTTTATAGCTCGCAGGCACT+TGG | + | chr3.4:66556084-66556103 | MS.gene048676:CDS | 45.0% |
| !!! | CTGCTTTTTATCTCAGGGCT+AGG | - | chr3.4:66556288-66556307 | None:intergenic | 45.0% |
| CGGTCATCCGATATTCTTGG+AGG | + | chr3.4:66556332-66556351 | MS.gene048676:CDS | 50.0% | |
| GAGACAGAACTGATGTGGCA+AGG | + | chr3.4:66556360-66556379 | MS.gene048676:CDS | 50.0% | |
| GCAAAGCGAGCTATGCGAAT+TGG | + | chr3.4:66556144-66556163 | MS.gene048676:CDS | 50.0% | |
| !! | GGGCTAGGCCTTATTGTTGA+TGG | - | chr3.4:66556273-66556292 | None:intergenic | 50.0% |
| TATAGCTCGCAGGCACTTGG+TGG | + | chr3.4:66556087-66556106 | MS.gene048676:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 66555793 | 66556512 | 66555793 | ID=MS.gene048676 |
| chr3.4 | mRNA | 66555793 | 66556512 | 66555793 | ID=MS.gene048676.t1;Parent=MS.gene048676 |
| chr3.4 | exon | 66555793 | 66556512 | 66555793 | ID=MS.gene048676.t1.exon1;Parent=MS.gene048676.t1 |
| chr3.4 | CDS | 66555793 | 66556512 | 66555793 | ID=cds.MS.gene048676.t1;Parent=MS.gene048676.t1 |
| Gene Sequence |
| Protein sequence |
