Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048698.t1 | KEH21988.1 | 54.3 | 223 | 52 | 3 | 1 | 220 | 1 | 176 | 1.30E-33 | 153.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048698.t1 | A0A072TYR1 | 54.3 | 223 | 52 | 3 | 1 | 220 | 1 | 176 | 9.7e-34 | 153.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene048698.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene048698.t1 | MTR_7g028855 | 60.000 | 225 | 40 | 5 | 1 | 222 | 1 | 178 | 3.38e-72 | 227 |
| MS.gene048698.t1 | MTR_7g446480 | 60.963 | 187 | 29 | 3 | 1 | 187 | 1 | 143 | 3.37e-61 | 194 |
| MS.gene048698.t1 | MTR_7g446520 | 58.480 | 171 | 36 | 2 | 56 | 226 | 15 | 150 | 3.16e-57 | 184 |
| MS.gene048698.t1 | MTR_8g467420 | 44.000 | 75 | 42 | 0 | 149 | 223 | 63 | 137 | 1.77e-18 | 79.7 |
| MS.gene048698.t1 | MTR_6g072520 | 46.667 | 120 | 44 | 3 | 1 | 119 | 1 | 101 | 9.11e-12 | 60.8 |
| MS.gene048698.t1 | MTR_3g080590 | 44.828 | 58 | 32 | 0 | 157 | 214 | 98 | 155 | 9.86e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 49 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAATGACTTTACGGATTT+TGG | 0.057115 | 3.2:-60834902 | MS.gene048698:CDS |
| CATTAACTGCTGAAGGAATA+AGG | 0.236547 | 3.2:-60834439 | MS.gene048698:CDS |
| AGTGATGATGGTGGTTGTTC+TGG | 0.317361 | 3.2:-60834674 | MS.gene048698:CDS |
| AAACAACAACAATGACTTTA+CGG | 0.325210 | 3.2:-60834909 | MS.gene048698:CDS |
| CTGTTAGGAAGAGTGATATT+AGG | 0.340554 | 3.2:-60834346 | MS.gene048698:CDS |
| GGTACACCGGAACATGATTA+CGG | 0.407134 | 3.2:-60834881 | MS.gene048698:CDS |
| AGCATTTATCAAGGTTAGAT+AGG | 0.409294 | 3.2:-60834255 | None:intergenic |
| TGCTGAAGGAATAAGGGAAA+TGG | 0.412429 | 3.2:-60834432 | MS.gene048698:CDS |
| GCTGATAGATATGATGAAAC+TGG | 0.418978 | 3.2:-60834707 | MS.gene048698:CDS |
| GATTATAGTGCTGGTGATAA+AGG | 0.421954 | 3.2:-60834650 | MS.gene048698:CDS |
| ATTAACTGCTGAAGGAATAA+GGG | 0.439851 | 3.2:-60834438 | MS.gene048698:CDS |
| GAGAGAAGAAGCATTTATCA+AGG | 0.445750 | 3.2:-60834264 | MS.gene048698:CDS |
| GATATTAGGCGCAATGTTAA+CGG | 0.449906 | 3.2:-60834332 | MS.gene048698:CDS |
| TGAGGCAGTATCTATGCAAT+AGG | 0.454261 | 3.2:-60834298 | MS.gene048698:CDS |
| TATAAGTACGGTAAGTGTAA+AGG | 0.460291 | 3.2:-60834374 | MS.gene048698:CDS |
| AAACATATCCGATGATTGTT+GGG | 0.461967 | 3.2:-60834832 | MS.gene048698:CDS |
| TGTAGTTGTTGGTGAGGAAA+TGG | 0.464499 | 3.2:-60834474 | MS.gene048698:CDS |
| GAAACATATCCGATGATTGT+TGG | 0.469215 | 3.2:-60834833 | MS.gene048698:CDS |
| TGATTACGGTTCTACGGATG+TGG | 0.481654 | 3.2:-60834867 | MS.gene048698:CDS |
| GAAGATGATAATGTAGTTGT+TGG | 0.483174 | 3.2:-60834485 | MS.gene048698:CDS |
| TGTTCTGGTGATTATAGTGC+TGG | 0.489646 | 3.2:-60834659 | MS.gene048698:CDS |
| GCAGTATCTATGCAATAGGC+AGG | 0.492780 | 3.2:-60834294 | MS.gene048698:CDS |
| GTAGAACCGTAATCATGTTC+CGG | 0.492886 | 3.2:+60834875 | None:intergenic |
| TACCATGACCTTCATTATCA+TGG | 0.501933 | 3.2:+60834531 | None:intergenic |
| ATAAATTCATTAACTGCTGA+AGG | 0.519495 | 3.2:-60834446 | MS.gene048698:CDS |
| GGAACATGATTACGGTTCTA+CGG | 0.552732 | 3.2:-60834873 | MS.gene048698:CDS |
| ACACCACTCCCAACAATCAT+CGG | 0.562059 | 3.2:+60834824 | None:intergenic |
| AGTGAAGGCCATGATAATGA+AGG | 0.569097 | 3.2:-60834539 | MS.gene048698:CDS |
| GTAGATGATGATGACAATGA+TGG | 0.572452 | 3.2:-60834773 | MS.gene048698:CDS |
| GGTGATGAAGGTGAAGGTGA+TGG | 0.578030 | 3.2:-60834593 | MS.gene048698:CDS |
| GATGAAAGTCAAGGTGATGA+AGG | 0.579998 | 3.2:-60834605 | MS.gene048698:CDS |
| AGTATCTATGCAATAGGCAG+GGG | 0.585986 | 3.2:-60834292 | MS.gene048698:CDS |
| GGTGATAAAGGTGAAGATGA+TGG | 0.589452 | 3.2:-60834638 | MS.gene048698:CDS |
| CAGTATCTATGCAATAGGCA+GGG | 0.594663 | 3.2:-60834293 | MS.gene048698:CDS |
| GGCCATGATAATGAAGGTCA+TGG | 0.596256 | 3.2:-60834533 | MS.gene048698:CDS |
| GGTGAAGGTGATGGAGGTGA+AGG | 0.600974 | 3.2:-60834584 | MS.gene048698:CDS |
| GGAGACAATGAAAGTGATGA+TGG | 0.609236 | 3.2:-60834686 | MS.gene048698:CDS |
| GATGAAGGTGAAGGTGATGG+AGG | 0.613749 | 3.2:-60834590 | MS.gene048698:CDS |
| AATGAAGGTCATGGTAGTGA+AGG | 0.627821 | 3.2:-60834524 | MS.gene048698:CDS |
| TAGATGATGATGACAATGAT+GGG | 0.643158 | 3.2:-60834772 | MS.gene048698:CDS |
| GATGACAATGATGGGAGTCA+TGG | 0.643643 | 3.2:-60834764 | MS.gene048698:CDS |
| TATCCGATGATTGTTGGGAG+TGG | 0.653545 | 3.2:-60834827 | MS.gene048698:CDS |
| TGATAATGTAGTTGTTGGTG+AGG | 0.656667 | 3.2:-60834480 | MS.gene048698:CDS |
| TTAACGGTGAGATTGTAATG+AGG | 0.680367 | 3.2:-60834316 | MS.gene048698:CDS |
| AGTCAAGGTGATGAAGGTGA+AGG | 0.696692 | 3.2:-60834599 | MS.gene048698:CDS |
| GACAATGAAAGTGATGATGG+TGG | 0.706532 | 3.2:-60834683 | MS.gene048698:CDS |
| GTATCTATGCAATAGGCAGG+GGG | 0.707463 | 3.2:-60834291 | MS.gene048698:CDS |
| AGTGAATGTGATGAAAGTCA+AGG | 0.717258 | 3.2:-60834614 | MS.gene048698:CDS |
| AGTGAAGATGATGAAAGTGA+AGG | 0.718504 | 3.2:-60834554 | MS.gene048698:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TACAATTTTTATTATAAGTA+CGG | - | chr3.2:60834784-60834803 | MS.gene048698:CDS | 10.0% |
| ! | AAACAACAACAATGACTTTA+CGG | - | chr3.2:60834261-60834280 | MS.gene048698:CDS | 25.0% |
| ! | ATAAATTCATTAACTGCTGA+AGG | - | chr3.2:60834724-60834743 | MS.gene048698:CDS | 25.0% |
| AAACATATCCGATGATTGTT+GGG | - | chr3.2:60834338-60834357 | MS.gene048698:CDS | 30.0% | |
| ATTAACTGCTGAAGGAATAA+GGG | - | chr3.2:60834732-60834751 | MS.gene048698:CDS | 30.0% | |
| TATAAGTACGGTAAGTGTAA+AGG | - | chr3.2:60834796-60834815 | MS.gene048698:CDS | 30.0% | |
| ! | AACAATGACTTTACGGATTT+TGG | - | chr3.2:60834268-60834287 | MS.gene048698:CDS | 30.0% |
| ! | AATTTTACGAGGAATACTCA+AGG | - | chr3.2:60834430-60834449 | MS.gene048698:CDS | 30.0% |
| ! | ATTTTACGAGGAATACTCAA+GGG | - | chr3.2:60834431-60834450 | MS.gene048698:CDS | 30.0% |
| ! | GAAGATGATAATGTAGTTGT+TGG | - | chr3.2:60834685-60834704 | MS.gene048698:CDS | 30.0% |
| ! | TAGATGATGATGACAATGAT+GGG | - | chr3.2:60834398-60834417 | MS.gene048698:CDS | 30.0% |
| AGTGAAGATGATGAAAGTGA+AGG | - | chr3.2:60834616-60834635 | MS.gene048698:CDS | 35.0% | |
| AGTGAATGTGATGAAAGTCA+AGG | - | chr3.2:60834556-60834575 | MS.gene048698:CDS | 35.0% | |
| CATTAACTGCTGAAGGAATA+AGG | - | chr3.2:60834731-60834750 | MS.gene048698:CDS | 35.0% | |
| CTGTTAGGAAGAGTGATATT+AGG | - | chr3.2:60834824-60834843 | MS.gene048698:CDS | 35.0% | |
| GAAACATATCCGATGATTGT+TGG | - | chr3.2:60834337-60834356 | MS.gene048698:CDS | 35.0% | |
| GATATTAGGCGCAATGTTAA+CGG | - | chr3.2:60834838-60834857 | MS.gene048698:CDS | 35.0% | |
| GCTGATAGATATGATGAAAC+TGG | - | chr3.2:60834463-60834482 | MS.gene048698:CDS | 35.0% | |
| TACCATGACCTTCATTATCA+TGG | + | chr3.2:60834642-60834661 | None:intergenic | 35.0% | |
| TTAACGGTGAGATTGTAATG+AGG | - | chr3.2:60834854-60834873 | MS.gene048698:CDS | 35.0% | |
| ! | GTAGATGATGATGACAATGA+TGG | - | chr3.2:60834397-60834416 | MS.gene048698:CDS | 35.0% |
| ! | TGATAATGTAGTTGTTGGTG+AGG | - | chr3.2:60834690-60834709 | MS.gene048698:CDS | 35.0% |
| !! | GAGAGAAGAAGCATTTATCA+AGG | - | chr3.2:60834906-60834925 | MS.gene048698:CDS | 35.0% |
| !! | GATTATAGTGCTGGTGATAA+AGG | - | chr3.2:60834520-60834539 | MS.gene048698:CDS | 35.0% |
| !!! | AGTGTAAAGGTTTTGCTGTT+AGG | - | chr3.2:60834809-60834828 | MS.gene048698:CDS | 35.0% |
| AGTATCTATGCAATAGGCAG+GGG | - | chr3.2:60834878-60834897 | MS.gene048698:CDS | 40.0% | |
| CAGTATCTATGCAATAGGCA+GGG | - | chr3.2:60834877-60834896 | MS.gene048698:CDS | 40.0% | |
| GATGAAAGTCAAGGTGATGA+AGG | - | chr3.2:60834565-60834584 | MS.gene048698:CDS | 40.0% | |
| GGAACATGATTACGGTTCTA+CGG | - | chr3.2:60834297-60834316 | MS.gene048698:CDS | 40.0% | |
| GGAGACAATGAAAGTGATGA+TGG | - | chr3.2:60834484-60834503 | MS.gene048698:CDS | 40.0% | |
| GGTGATAAAGGTGAAGATGA+TGG | - | chr3.2:60834532-60834551 | MS.gene048698:CDS | 40.0% | |
| GTAGAACCGTAATCATGTTC+CGG | + | chr3.2:60834298-60834317 | None:intergenic | 40.0% | |
| TGAGGCAGTATCTATGCAAT+AGG | - | chr3.2:60834872-60834891 | MS.gene048698:CDS | 40.0% | |
| TGCTGAAGGAATAAGGGAAA+TGG | - | chr3.2:60834738-60834757 | MS.gene048698:CDS | 40.0% | |
| TGTTCTGGTGATTATAGTGC+TGG | - | chr3.2:60834511-60834530 | MS.gene048698:CDS | 40.0% | |
| ! | AATGAAGGTCATGGTAGTGA+AGG | - | chr3.2:60834646-60834665 | MS.gene048698:CDS | 40.0% |
| ! | AGTGAAGGCCATGATAATGA+AGG | - | chr3.2:60834631-60834650 | MS.gene048698:CDS | 40.0% |
| ! | GGAGTCATGGTAATTTTACG+AGG | - | chr3.2:60834419-60834438 | MS.gene048698:CDS | 40.0% |
| ! | TGTAGTTGTTGGTGAGGAAA+TGG | - | chr3.2:60834696-60834715 | MS.gene048698:CDS | 40.0% |
| !! | CTTTACGGATTTTGGTACAC+CGG | - | chr3.2:60834276-60834295 | MS.gene048698:CDS | 40.0% |
| !! | GACAATGAAAGTGATGATGG+TGG | - | chr3.2:60834487-60834506 | MS.gene048698:CDS | 40.0% |
| ACACCACTCCCAACAATCAT+CGG | + | chr3.2:60834349-60834368 | None:intergenic | 45.0% | |
| AGTCAAGGTGATGAAGGTGA+AGG | - | chr3.2:60834571-60834590 | MS.gene048698:CDS | 45.0% | |
| GCAGTATCTATGCAATAGGC+AGG | - | chr3.2:60834876-60834895 | MS.gene048698:CDS | 45.0% | |
| GGTACACCGGAACATGATTA+CGG | - | chr3.2:60834289-60834308 | MS.gene048698:CDS | 45.0% | |
| GTATCTATGCAATAGGCAGG+GGG | - | chr3.2:60834879-60834898 | MS.gene048698:CDS | 45.0% | |
| TATCCGATGATTGTTGGGAG+TGG | - | chr3.2:60834343-60834362 | MS.gene048698:CDS | 45.0% | |
| TGATTACGGTTCTACGGATG+TGG | - | chr3.2:60834303-60834322 | MS.gene048698:CDS | 45.0% | |
| ! | GATGACAATGATGGGAGTCA+TGG | - | chr3.2:60834406-60834425 | MS.gene048698:CDS | 45.0% |
| ! | GGCCATGATAATGAAGGTCA+TGG | - | chr3.2:60834637-60834656 | MS.gene048698:CDS | 45.0% |
| !! | AGTGATGATGGTGGTTGTTC+TGG | - | chr3.2:60834496-60834515 | MS.gene048698:CDS | 45.0% |
| GGTGATGAAGGTGAAGGTGA+TGG | - | chr3.2:60834577-60834596 | MS.gene048698:CDS | 50.0% | |
| ! | GATGAAGGTGAAGGTGATGG+AGG | - | chr3.2:60834580-60834599 | MS.gene048698:CDS | 50.0% |
| !! | GGTGAAGGTGATGGAGGTGA+AGG | - | chr3.2:60834586-60834605 | MS.gene048698:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 60834256 | 60834936 | 60834256 | ID=MS.gene048698 |
| chr3.2 | mRNA | 60834256 | 60834936 | 60834256 | ID=MS.gene048698.t1;Parent=MS.gene048698 |
| chr3.2 | exon | 60834256 | 60834936 | 60834256 | ID=MS.gene048698.t1.exon1;Parent=MS.gene048698.t1 |
| chr3.2 | CDS | 60834256 | 60834936 | 60834256 | ID=cds.MS.gene048698.t1;Parent=MS.gene048698.t1 |
| Gene Sequence |
| Protein sequence |
