Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049303.t1 | AES72826.1 | 94 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 3.70E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049303.t1 | B9RU15 | 65.3 | 72 | 25 | 0 | 39 | 110 | 38 | 109 | 1.3e-25 | 117.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049303.t1 | G7JB04 | 94.0 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 2.7e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene049303.t1 | TR | PHD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049303.t1 | MTR_3g095830 | 94.017 | 117 | 7 | 0 | 1 | 117 | 1 | 117 | 4.73e-79 | 228 |
| MS.gene049303.t1 | MTR_3g095840 | 58.407 | 113 | 39 | 2 | 3 | 107 | 8 | 120 | 1.40e-37 | 130 |
| MS.gene049303.t1 | MTR_1g007670 | 45.312 | 64 | 35 | 0 | 44 | 107 | 10 | 73 | 2.77e-15 | 70.5 |
| MS.gene049303.t1 | MTR_5g010300 | 36.842 | 95 | 57 | 1 | 18 | 109 | 197 | 291 | 5.14e-14 | 67.4 |
| MS.gene049303.t1 | MTR_5g010300 | 36.842 | 95 | 57 | 1 | 18 | 109 | 197 | 291 | 5.37e-14 | 67.0 |
| MS.gene049303.t1 | MTR_5g010300 | 36.842 | 95 | 57 | 1 | 18 | 109 | 197 | 291 | 5.45e-14 | 67.0 |
| MS.gene049303.t1 | MTR_5g010300 | 36.842 | 95 | 57 | 1 | 18 | 109 | 197 | 291 | 5.69e-14 | 67.0 |
| MS.gene049303.t1 | MTR_1g007700 | 50.000 | 48 | 20 | 1 | 60 | 107 | 258 | 301 | 2.70e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049303.t1 | AT5G09790 | 50.000 | 100 | 42 | 2 | 16 | 110 | 18 | 114 | 3.73e-25 | 97.8 |
| MS.gene049303.t1 | AT5G09790 | 50.000 | 100 | 42 | 2 | 16 | 110 | 18 | 114 | 3.81e-25 | 97.8 |
| MS.gene049303.t1 | AT5G24330 | 69.565 | 46 | 14 | 0 | 62 | 107 | 34 | 79 | 1.08e-18 | 79.7 |
| MS.gene049303.t1 | AT1G63490 | 41.772 | 79 | 43 | 1 | 31 | 109 | 213 | 288 | 8.01e-13 | 63.9 |
| MS.gene049303.t1 | AT1G63490 | 41.772 | 79 | 43 | 1 | 31 | 109 | 213 | 288 | 8.17e-13 | 63.9 |
Find 36 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTAGAGTTCCTATTGGTTCA+TGG | 0.294500 | 3.3:+79532749 | MS.gene049303:CDS |
| AGTCTGTTGGCTTCTTCTTT+CGG | 0.305637 | 3.3:-79532534 | None:intergenic |
| GAAGTGTCTGAGACCGGTTC+TGG | 0.362102 | 3.3:+79532726 | MS.gene049303:CDS |
| CCCCAAGTGTTGTTCTGAGC+TGG | 0.393884 | 3.3:+79532777 | MS.gene049303:CDS |
| GGGCAAATCCATGAACCAAT+AGG | 0.417784 | 3.3:-79532757 | None:intergenic |
| GGCTTCTTCTTTCGGTGGCA+TGG | 0.424482 | 3.3:-79532526 | None:intergenic |
| GCATGGTGCATGTGTGCGGC+GGG | 0.438614 | 3.3:-79532509 | None:intergenic |
| CAGGACGATGACTAATATAA+TGG | 0.457022 | 3.3:+79532579 | MS.gene049303:CDS |
| CTCCAGCTCAGAACAACACT+TGG | 0.460665 | 3.3:-79532779 | None:intergenic |
| GTTCTGGCTAGAGTTCCTAT+TGG | 0.469825 | 3.3:+79532742 | MS.gene049303:CDS |
| GTGAAGTTGGTTAGCTTACG+AGG | 0.474869 | 3.3:-79532487 | None:intergenic |
| ATGTGTGAGCGATGTGGATC+TGG | 0.479905 | 3.3:+79532649 | MS.gene049303:CDS |
| ATAGGAACTCTAGCCAGAAC+CGG | 0.497595 | 3.3:-79532739 | None:intergenic |
| TCACACAACAAAAGCTCCTC+AGG | 0.503103 | 3.3:-79532679 | None:intergenic |
| CTGTTGGCTTCTTCTTTCGG+TGG | 0.523013 | 3.3:-79532531 | None:intergenic |
| TCATATGAAGTGTCTGAGAC+CGG | 0.525057 | 3.3:+79532720 | MS.gene049303:CDS |
| GCCAACAGACTCGTCATCGC+GGG | 0.543998 | 3.3:+79532546 | MS.gene049303:CDS |
| TCCAGCTCAGAACAACACTT+GGG | 0.544872 | 3.3:-79532778 | None:intergenic |
| ATGTGTGCGGCGGGTGAAGT+TGG | 0.552519 | 3.3:-79532500 | None:intergenic |
| GGCATGGTGCATGTGTGCGG+CGG | 0.561299 | 3.3:-79532510 | None:intergenic |
| ATATGCAGTGGTTGAGAAGG+AGG | 0.563550 | 3.3:+79532612 | MS.gene049303:CDS |
| CATCGCGGGTGACGAAGTAC+AGG | 0.571128 | 3.3:+79532560 | MS.gene049303:CDS |
| TAAATATGCAGTGGTTGAGA+AGG | 0.574113 | 3.3:+79532609 | MS.gene049303:CDS |
| ACCCGCGATGACGAGTCTGT+TGG | 0.610544 | 3.3:-79532547 | None:intergenic |
| TTGTGTGACAAATGCGACAA+AGG | 0.628248 | 3.3:+79532694 | MS.gene049303:CDS |
| CGATGACTAATATAATGGCG+AGG | 0.630890 | 3.3:+79532584 | MS.gene049303:CDS |
| TGAAGTTGGTTAGCTTACGA+GGG | 0.634466 | 3.3:-79532486 | None:intergenic |
| GATGACTAATATAATGGCGA+GGG | 0.645664 | 3.3:+79532585 | MS.gene049303:CDS |
| AGCCAACAGACTCGTCATCG+CGG | 0.654231 | 3.3:+79532545 | MS.gene049303:CDS |
| GGCGAGGGCTAAATATGCAG+TGG | 0.659115 | 3.3:+79532600 | MS.gene049303:CDS |
| GGTGGCATGGTGCATGTGTG+CGG | 0.659596 | 3.3:-79532513 | None:intergenic |
| GAAGTTGGTTAGCTTACGAG+GGG | 0.667366 | 3.3:-79532485 | None:intergenic |
| CCAGCTCAGAACAACACTTG+GGG | 0.668343 | 3.3:-79532777 | None:intergenic |
| GACTGCATGTGTGAGCGATG+TGG | 0.685024 | 3.3:+79532643 | MS.gene049303:CDS |
| TGGATCTGGTGAACAACCTG+AGG | 0.697540 | 3.3:+79532663 | MS.gene049303:CDS |
| AAGTTGGTTAGCTTACGAGG+GGG | 0.753964 | 3.3:-79532484 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CAGGACGATGACTAATATAA+TGG | + | chr3.3:79532579-79532598 | MS.gene049303:CDS | 35.0% | |
| GATGACTAATATAATGGCGA+GGG | + | chr3.3:79532585-79532604 | MS.gene049303:CDS | 35.0% | |
| TAAATATGCAGTGGTTGAGA+AGG | + | chr3.3:79532609-79532628 | MS.gene049303:CDS | 35.0% | |
| CGATGACTAATATAATGGCG+AGG | + | chr3.3:79532584-79532603 | MS.gene049303:CDS | 40.0% | |
| TTGTGTGACAAATGCGACAA+AGG | + | chr3.3:79532694-79532713 | MS.gene049303:CDS | 40.0% | |
| ! | TCATATGAAGTGTCTGAGAC+CGG | + | chr3.3:79532720-79532739 | MS.gene049303:CDS | 40.0% |
| !! | AGTCTGTTGGCTTCTTCTTT+CGG | - | chr3.3:79532537-79532556 | None:intergenic | 40.0% |
| !! | CTAGAGTTCCTATTGGTTCA+TGG | + | chr3.3:79532749-79532768 | MS.gene049303:CDS | 40.0% |
| !! | TGAAGTTGGTTAGCTTACGA+GGG | - | chr3.3:79532489-79532508 | None:intergenic | 40.0% |
| ATAGGAACTCTAGCCAGAAC+CGG | - | chr3.3:79532742-79532761 | None:intergenic | 45.0% | |
| ATATGCAGTGGTTGAGAAGG+AGG | + | chr3.3:79532612-79532631 | MS.gene049303:CDS | 45.0% | |
| GGGCAAATCCATGAACCAAT+AGG | - | chr3.3:79532760-79532779 | None:intergenic | 45.0% | |
| TCACACAACAAAAGCTCCTC+AGG | - | chr3.3:79532682-79532701 | None:intergenic | 45.0% | |
| TCCAGCTCAGAACAACACTT+GGG | - | chr3.3:79532781-79532800 | None:intergenic | 45.0% | |
| ! | GTTCTGGCTAGAGTTCCTAT+TGG | + | chr3.3:79532742-79532761 | MS.gene049303:CDS | 45.0% |
| !! | AAGTTGGTTAGCTTACGAGG+GGG | - | chr3.3:79532487-79532506 | None:intergenic | 45.0% |
| !! | GAAGTTGGTTAGCTTACGAG+GGG | - | chr3.3:79532488-79532507 | None:intergenic | 45.0% |
| !! | GTGAAGTTGGTTAGCTTACG+AGG | - | chr3.3:79532490-79532509 | None:intergenic | 45.0% |
| ATGTGTGAGCGATGTGGATC+TGG | + | chr3.3:79532649-79532668 | MS.gene049303:CDS | 50.0% | |
| CCAGCTCAGAACAACACTTG+GGG | - | chr3.3:79532780-79532799 | None:intergenic | 50.0% | |
| CTCCAGCTCAGAACAACACT+TGG | - | chr3.3:79532782-79532801 | None:intergenic | 50.0% | |
| TGGATCTGGTGAACAACCTG+AGG | + | chr3.3:79532663-79532682 | MS.gene049303:CDS | 50.0% | |
| !! | CTGTTGGCTTCTTCTTTCGG+TGG | - | chr3.3:79532534-79532553 | None:intergenic | 50.0% |
| AGCCAACAGACTCGTCATCG+CGG | + | chr3.3:79532545-79532564 | MS.gene049303:CDS | 55.0% | |
| GACTGCATGTGTGAGCGATG+TGG | + | chr3.3:79532643-79532662 | MS.gene049303:CDS | 55.0% | |
| GGCGAGGGCTAAATATGCAG+TGG | + | chr3.3:79532600-79532619 | MS.gene049303:CDS | 55.0% | |
| GGCTTCTTCTTTCGGTGGCA+TGG | - | chr3.3:79532529-79532548 | None:intergenic | 55.0% | |
| ! | CCCCAAGTGTTGTTCTGAGC+TGG | + | chr3.3:79532777-79532796 | MS.gene049303:CDS | 55.0% |
| ! | GAAGTGTCTGAGACCGGTTC+TGG | + | chr3.3:79532726-79532745 | MS.gene049303:CDS | 55.0% |
| !! | TTGGTTAGCTTACGAGGGGG+AGG | - | chr3.3:79532484-79532503 | None:intergenic | 55.0% |
| ACCCGCGATGACGAGTCTGT+TGG | - | chr3.3:79532550-79532569 | None:intergenic | 60.0% | |
| ATGTGTGCGGCGGGTGAAGT+TGG | - | chr3.3:79532503-79532522 | None:intergenic | 60.0% | |
| CATCGCGGGTGACGAAGTAC+AGG | + | chr3.3:79532560-79532579 | MS.gene049303:CDS | 60.0% | |
| GCCAACAGACTCGTCATCGC+GGG | + | chr3.3:79532546-79532565 | MS.gene049303:CDS | 60.0% | |
| GGTGGCATGGTGCATGTGTG+CGG | - | chr3.3:79532516-79532535 | None:intergenic | 60.0% | |
| ! | GTTAGCTTACGAGGGGGAGG+TGG | - | chr3.3:79532481-79532500 | None:intergenic | 60.0% |
| GCATGGTGCATGTGTGCGGC+GGG | - | chr3.3:79532512-79532531 | None:intergenic | 65.0% | |
| GGCATGGTGCATGTGTGCGG+CGG | - | chr3.3:79532513-79532532 | None:intergenic | 65.0% | |
| CTTACGAGGGGGAGGTGGCG+AGG | - | chr3.3:79532476-79532495 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 79532466 | 79532819 | 79532466 | ID=MS.gene049303 |
| chr3.3 | mRNA | 79532466 | 79532819 | 79532466 | ID=MS.gene049303.t1;Parent=MS.gene049303 |
| chr3.3 | exon | 79532466 | 79532819 | 79532466 | ID=MS.gene049303.t1.exon1;Parent=MS.gene049303.t1 |
| chr3.3 | CDS | 79532466 | 79532819 | 79532466 | ID=cds.MS.gene049303.t1;Parent=MS.gene049303.t1 |
| Gene Sequence |
| Protein sequence |
