AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049461


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049461.t1 MTR_5g082910 89.041 73 8 0 16 88 5 77 2.37e-41 138
MS.gene049461.t1 MTR_5g016510 65.574 61 21 0 27 87 2 62 1.72e-23 92.8
MS.gene049461.t1 MTR_3g101290 52.564 78 33 1 27 104 34 107 1.02e-22 89.0
MS.gene049461.t1 MTR_4g094982 63.934 61 22 0 27 87 2 62 1.36e-22 89.4
MS.gene049461.t1 MTR_6g015455 62.295 61 23 0 27 87 2 62 3.90e-20 85.1
MS.gene049461.t1 MTR_4g019370 61.017 59 23 0 28 86 36 94 5.30e-19 80.9
MS.gene049461.t1 MTR_6g027340 39.130 69 42 0 18 86 4 72 1.88e-13 64.3
MS.gene049461.t1 MTR_5g010650 46.774 62 33 0 25 86 105 166 4.62e-13 64.7
MS.gene049461.t1 MTR_3g089045 49.123 57 29 0 30 86 16 72 9.04e-13 63.5
MS.gene049461.t1 MTR_6g027360 39.130 69 42 0 18 86 66 134 9.65e-13 63.5
MS.gene049461.t1 MTR_6g027370 48.276 58 30 0 29 86 98 155 1.33e-12 63.2
MS.gene049461.t1 MTR_3g110028 48.214 56 29 0 25 80 80 135 2.80e-12 62.4
MS.gene049461.t1 MTR_6g074860 47.368 57 30 0 30 86 4 60 6.44e-12 60.5
MS.gene049461.t1 MTR_5g488170 49.180 61 30 1 27 86 11 71 1.90e-11 59.3
MS.gene049461.t1 MTR_5g088080 49.180 61 30 1 27 86 11 71 2.05e-11 59.3
MS.gene049461.t1 MTR_5g088150 49.180 61 30 1 27 86 11 71 2.05e-11 59.3
MS.gene049461.t1 MTR_5g488210 49.180 61 30 1 27 86 11 71 2.05e-11 59.3
MS.gene049461.t1 MTR_5g088640 49.180 61 30 1 27 86 11 71 2.06e-11 59.7
MS.gene049461.t1 MTR_1g026870 44.643 56 31 0 25 80 80 135 2.61e-11 59.7
MS.gene049461.t1 MTR_5g041570 40.000 75 41 1 16 86 116 190 2.62e-11 59.7
MS.gene049461.t1 MTR_5g062790 49.180 61 30 1 27 86 8 68 3.04e-11 59.3
MS.gene049461.t1 MTR_3g077650 47.170 53 28 0 29 81 180 232 5.13e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049461.t1 AT3G50060 53.165 79 33 1 26 104 2 76 2.95e-23 92.0
MS.gene049461.t1 AT5G67300 63.333 60 22 0 27 86 3 62 1.73e-21 87.0
MS.gene049461.t1 AT2G23290 52.564 78 33 1 27 104 10 83 6.24e-21 85.9
MS.gene049461.t1 AT4G37260 57.143 63 27 0 24 86 7 69 4.16e-20 83.6
MS.gene049461.t1 AT2G39880 52.703 74 31 1 13 86 37 106 5.77e-20 84.0
MS.gene049461.t1 AT3G55730 59.322 59 24 0 28 86 54 112 5.00e-19 81.3
MS.gene049461.t1 AT3G09230 57.353 68 29 0 19 86 44 111 1.46e-18 80.1
MS.gene049461.t1 AT1G73410 49.123 57 29 0 30 86 6 62 6.28e-13 62.8
MS.gene049461.t1 AT1G73410 49.123 57 29 0 30 86 6 62 6.68e-13 63.2
MS.gene049461.t1 AT1G17950 47.368 57 30 0 30 86 5 61 5.73e-12 60.8
MS.gene049461.t1 AT4G33450 45.614 57 31 0 30 86 19 75 6.40e-12 60.8
MS.gene049461.t1 AT5G11050 45.283 53 29 0 29 81 104 156 1.24e-11 60.5
MS.gene049461.t1 AT4G32730 48.214 56 29 0 25 80 82 137 1.45e-11 60.5
MS.gene049461.t1 AT4G32730 48.214 56 29 0 25 80 82 137 1.45e-11 60.5
MS.gene049461.t1 AT4G32730 48.214 56 29 0 25 80 82 137 1.45e-11 60.5
MS.gene049461.t1 AT4G32730 48.214 56 29 0 25 80 82 137 1.45e-11 60.5
MS.gene049461.t1 AT4G32730 48.214 56 29 0 25 80 82 137 1.62e-11 60.5
MS.gene049461.t1 AT3G09370 44.643 56 31 0 25 80 138 193 1.66e-11 60.5
MS.gene049461.t1 AT3G09370 44.643 56 31 0 25 80 125 180 1.71e-11 60.1
MS.gene049461.t1 AT3G09370 44.643 56 31 0 25 80 130 185 1.73e-11 60.1
MS.gene049461.t1 AT5G11510 44.643 56 31 0 25 80 76 131 2.01e-11 60.1
MS.gene049461.t1 AT5G11510 44.643 56 31 0 25 80 76 131 2.01e-11 60.1
MS.gene049461.t1 AT5G11510 44.643 56 31 0 25 80 76 131 2.01e-11 60.1
MS.gene049461.t1 AT5G17800 47.368 57 30 0 30 86 93 149 2.17e-11 59.7
MS.gene049461.t1 AT5G02320 45.614 57 31 0 25 81 122 178 2.19e-11 60.1
MS.gene049461.t1 AT5G02320 45.614 57 31 0 25 81 122 178 2.19e-11 60.1
MS.gene049461.t1 AT5G02320 43.548 62 35 0 25 86 62 123 2.32e-11 59.7
MS.gene049461.t1 AT5G11510 44.643 56 31 0 25 80 76 131 2.35e-11 60.1
MS.gene049461.t1 AT5G11510 44.643 56 31 0 25 80 76 131 2.51e-11 59.7
MS.gene049461.t1 AT5G58850 41.509 53 31 0 29 81 104 156 3.87e-11 59.3

Find 28 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAAAATTTCCATATGTTT+TGG 0.296719 3.2:+56618872 MS.gene049461:intron
GCCACCACCGCCGGTGTAAA+AGG 0.337215 3.2:-56618409 None:intergenic
AACGGAATCCCCGATCGTTC+TGG 0.385664 3.2:+56618331 MS.gene049461:CDS
AGGAAAACGGTGCAAGAAAC+TGG 0.414736 3.2:+56618296 MS.gene049461:CDS
TCAAAAGGAACCAAAACATA+TGG 0.425019 3.2:-56618882 None:intergenic
ACCACCGTTTGAATTCGAAT+TGG 0.426843 3.2:-56618197 None:intergenic
AGATGAAACATTGGTGAAAC+TGG 0.432466 3.2:+56618270 MS.gene049461:CDS
ACGGAATCCCCGATCGTTCT+GGG 0.435316 3.2:+56618332 MS.gene049461:CDS
TCGAATTCAAACGGTGGTGG+TGG 0.444499 3.2:+56618202 MS.gene049461:CDS
GTGATGAAGTGAAGAGTTCA+TGG 0.455401 3.2:+56618236 MS.gene049461:CDS
CAAAACTTCCCAGAACGATC+GGG 0.482750 3.2:-56618340 None:intergenic
ATGGCCAAGGGACGATCAAA+AGG 0.494112 3.2:-56618897 None:intergenic
CGAATTCAAACGGTGGTGGT+GGG 0.506583 3.2:+56618203 MS.gene049461:CDS
GCAAAACTTCCCAGAACGAT+CGG 0.556052 3.2:-56618341 None:intergenic
AGTACCAATTCGAATTCAAA+CGG 0.564007 3.2:+56618193 MS.gene049461:CDS
GAATTCAAACGGTGGTGGTG+GGG 0.567365 3.2:+56618204 MS.gene049461:CDS
AAAGGAAGGTGTTGAACGGT+TGG 0.567448 3.2:-56618391 None:intergenic
GGTGGTGGTGGGGATGAAAA+CGG 0.571134 3.2:+56618214 MS.gene049461:CDS
CCACCGCCGGTGTAAAAGGA+AGG 0.581661 3.2:-56618405 None:intergenic
GATTGTTGTGCCACCACCGC+CGG 0.590963 3.2:-56618418 None:intergenic
AACTGGTCTGTGATAAGCAA+CGG 0.591578 3.2:+56618313 MS.gene049461:CDS
GTGAAACTGGTGAAGGAAAA+CGG 0.592557 3.2:+56618283 MS.gene049461:CDS
GCTGATTACACCACCGCAGA+CGG 0.606009 3.2:-56618363 None:intergenic
AATTCGAATTCAAACGGTGG+TGG 0.622740 3.2:+56618199 MS.gene049461:CDS
TGTAAAAGGAAGGTGTTGAA+CGG 0.650810 3.2:-56618395 None:intergenic
AACATTGGTGAAACTGGTGA+AGG 0.661429 3.2:+56618276 MS.gene049461:CDS
AAAACTTCCCAGAACGATCG+GGG 0.663763 3.2:-56618339 None:intergenic
ACCAATTCGAATTCAAACGG+TGG 0.728179 3.2:+56618196 MS.gene049461:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTTTTTGTTAAAATGT+AGG + chr3.2:56618851-56618870 MS.gene049461:intron 15.0%
!! ATATATGTGTAAAGTGTAAT+TGG + chr3.2:56618792-56618811 MS.gene049461:intron 20.0%
! TAAAAACGAAGATGAAACAT+TGG + chr3.2:56618261-56618280 MS.gene049461:CDS 25.0%
!! GGTAAAATTTCCATATGTTT+TGG + chr3.2:56618872-56618891 MS.gene049461:intron 25.0%
!!! ATTCTTCGATTTTTTGGAAT+GGG + chr3.2:56618473-56618492 MS.gene049461:intron 25.0%
ACGAGATTCAATCCTAAAAA+TGG - chr3.2:56618919-56618938 None:intergenic 30.0%
AGTACCAATTCGAATTCAAA+CGG + chr3.2:56618193-56618212 MS.gene049461:CDS 30.0%
CAAAAATCAGCAATGTTGTA+AGG - chr3.2:56618630-56618649 None:intergenic 30.0%
TCAAAAGGAACCAAAACATA+TGG - chr3.2:56618885-56618904 None:intergenic 30.0%
TCCAAAAAATCGAAGAATCT+TGG - chr3.2:56618471-56618490 None:intergenic 30.0%
!! TGATAGCTTTGTTAAAGTCT+TGG + chr3.2:56618738-56618757 MS.gene049461:intron 30.0%
!!! ACCAAGATTCTTCGATTTTT+TGG + chr3.2:56618467-56618486 MS.gene049461:intron 30.0%
!!! GATTCTTCGATTTTTTGGAA+TGG + chr3.2:56618472-56618491 MS.gene049461:intron 30.0%
AAGCTTTACCAAGCAATTTG+AGG - chr3.2:56618693-56618712 None:intergenic 35.0%
AGAAGAAGAAGAAGAGAAGA+AGG + chr3.2:56618580-56618599 MS.gene049461:intron 35.0%
AGATGAAACATTGGTGAAAC+TGG + chr3.2:56618270-56618289 MS.gene049461:CDS 35.0%
GTCTGATTCAGAGTGAAATT+CGG + chr3.2:56618497-56618516 MS.gene049461:intron 35.0%
TCAATCCTAAAAATGGCCAA+GGG - chr3.2:56618912-56618931 None:intergenic 35.0%
TCTGATTCAGAGTGAAATTC+GGG + chr3.2:56618498-56618517 MS.gene049461:intron 35.0%
TTCAATCCTAAAAATGGCCA+AGG - chr3.2:56618913-56618932 None:intergenic 35.0%
TTCTTCTTCTTTCCTTCTTC+TGG - chr3.2:56618570-56618589 None:intergenic 35.0%
TTTACGTGTTAGAATCGCTT+AGG + chr3.2:56618766-56618785 MS.gene049461:intron 35.0%
!! TGTAAAAGGAAGGTGTTGAA+CGG - chr3.2:56618398-56618417 None:intergenic 35.0%
AACATTGGTGAAACTGGTGA+AGG + chr3.2:56618276-56618295 MS.gene049461:CDS 40.0%
AATTCGAATTCAAACGGTGG+TGG + chr3.2:56618199-56618218 MS.gene049461:CDS 40.0%
ACCAATTCGAATTCAAACGG+TGG + chr3.2:56618196-56618215 MS.gene049461:CDS 40.0%
ACCACCGTTTGAATTCGAAT+TGG - chr3.2:56618200-56618219 None:intergenic 40.0%
ATCAGCAATGTTGTAAGGCA+AGG - chr3.2:56618625-56618644 None:intergenic 40.0%
GGAAGAAGAAGAAGAAGTGA+CGG + chr3.2:56618519-56618538 MS.gene049461:intron 40.0%
GTGAAACTGGTGAAGGAAAA+CGG + chr3.2:56618283-56618302 MS.gene049461:CDS 40.0%
GTGATGAAGTGAAGAGTTCA+TGG + chr3.2:56618236-56618255 MS.gene049461:CDS 40.0%
! AACTGGTCTGTGATAAGCAA+CGG + chr3.2:56618313-56618332 MS.gene049461:CDS 40.0%
! ACCAAGCAATTTGAGGCAAA+CGG - chr3.2:56618686-56618705 None:intergenic 40.0%
! TCAACACCTTCCTTTTACAC+CGG + chr3.2:56618399-56618418 MS.gene049461:CDS 40.0%
!! TCTTTCCTTCTTCTGGTTTG+CGG - chr3.2:56618563-56618582 None:intergenic 40.0%
AAAACTTCCCAGAACGATCG+GGG - chr3.2:56618342-56618361 None:intergenic 45.0%
AAACCTACTTGGCAAGAAGG+AGG - chr3.2:56618663-56618682 None:intergenic 45.0%
AACCTACTTGGCAAGAAGGA+GGG - chr3.2:56618662-56618681 None:intergenic 45.0%
AGAAGAAGAAGAAGTGACGG+TGG + chr3.2:56618522-56618541 MS.gene049461:intron 45.0%
AGGAAAACGGTGCAAGAAAC+TGG + chr3.2:56618296-56618315 MS.gene049461:CDS 45.0%
CAAAACTTCCCAGAACGATC+GGG - chr3.2:56618343-56618362 None:intergenic 45.0%
GCAAAACTTCCCAGAACGAT+CGG - chr3.2:56618344-56618363 None:intergenic 45.0%
TCCGTTTGCCTCAAATTGCT+TGG + chr3.2:56618682-56618701 MS.gene049461:intron 45.0%
!! AAAGGAAGGTGTTGAACGGT+TGG - chr3.2:56618394-56618413 None:intergenic 45.0%
!!! ATCGTCCCTTGGCCATTTTT+AGG + chr3.2:56618904-56618923 MS.gene049461:CDS 45.0%
AATGTTGTAAGGCAAGGCCC+AGG - chr3.2:56618619-56618638 None:intergenic 50.0%
AGAAGAAGAAGTGACGGTGG+AGG + chr3.2:56618525-56618544 MS.gene049461:intron 50.0%
ATGGCCAAGGGACGATCAAA+AGG - chr3.2:56618900-56618919 None:intergenic 50.0%
CGGAAACCTACTTGGCAAGA+AGG - chr3.2:56618666-56618685 None:intergenic 50.0%
GAAGAAGAAGTGACGGTGGA+GGG + chr3.2:56618526-56618545 MS.gene049461:intron 50.0%
GAGGCAAACGGAAACCTACT+TGG - chr3.2:56618674-56618693 None:intergenic 50.0%
! ACACCTTCCTTTTACACCGG+CGG + chr3.2:56618402-56618421 MS.gene049461:CDS 50.0%
! CGAATTCAAACGGTGGTGGT+GGG + chr3.2:56618203-56618222 MS.gene049461:CDS 50.0%
! GAATTCAAACGGTGGTGGTG+GGG + chr3.2:56618204-56618223 MS.gene049461:CDS 50.0%
! GGTTCCTTTTGATCGTCCCT+TGG + chr3.2:56618893-56618912 MS.gene049461:CDS 50.0%
! TCGAATTCAAACGGTGGTGG+TGG + chr3.2:56618202-56618221 MS.gene049461:CDS 50.0%
!! AGAGAAGAAGGAGTGCTACG+TGG + chr3.2:56618592-56618611 MS.gene049461:intron 50.0%
!! GAGAAGAAGGAGTGCTACGT+GGG + chr3.2:56618593-56618612 MS.gene049461:intron 50.0%
AACGGAATCCCCGATCGTTC+TGG + chr3.2:56618331-56618350 MS.gene049461:CDS 55.0%
AAGTGACGGTGGAGGGAGAA+AGG + chr3.2:56618533-56618552 MS.gene049461:intron 55.0%
ACGGAATCCCCGATCGTTCT+GGG + chr3.2:56618332-56618351 MS.gene049461:CDS 55.0%
CACCCTCCTTCTTGCCAAGT+AGG + chr3.2:56618657-56618676 MS.gene049461:intron 55.0%
GACTGCCGCAAACCAGAAGA+AGG + chr3.2:56618555-56618574 MS.gene049461:intron 55.0%
GCTGATTACACCACCGCAGA+CGG - chr3.2:56618366-56618385 None:intergenic 55.0%
! CTGGGAAGTTTTGCCGTCTG+CGG + chr3.2:56618350-56618369 MS.gene049461:CDS 55.0%
!! GGTGGTGGTGGGGATGAAAA+CGG + chr3.2:56618214-56618233 MS.gene049461:CDS 55.0%
CCACCGCCGGTGTAAAAGGA+AGG - chr3.2:56618408-56618427 None:intergenic 60.0%
GATTGTTGTGCCACCACCGC+CGG - chr3.2:56618421-56618440 None:intergenic 60.0%
! CCTTCCTTTTACACCGGCGG+TGG + chr3.2:56618405-56618424 MS.gene049461:CDS 60.0%
! CTTGGCAAGAAGGAGGGTGC+AGG - chr3.2:56618656-56618675 None:intergenic 60.0%
! GGAAGTTTTGCCGTCTGCGG+TGG + chr3.2:56618353-56618372 MS.gene049461:CDS 60.0%
! TCCTTTTACACCGGCGGTGG+TGG + chr3.2:56618408-56618427 MS.gene049461:CDS 60.0%
!! AAGGAGTGCTACGTGGGCCT+GGG + chr3.2:56618599-56618618 MS.gene049461:intron 60.0%
GCCACCACCGCCGGTGTAAA+AGG - chr3.2:56618412-56618431 None:intergenic 65.0%
!! GAAGGAGTGCTACGTGGGCC+TGG + chr3.2:56618598-56618617 MS.gene049461:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 56618160 56618944 56618160 ID=MS.gene049461
chr3.2 mRNA 56618160 56618944 56618160 ID=MS.gene049461.t1;Parent=MS.gene049461
chr3.2 exon 56618160 56618462 56618160 ID=MS.gene049461.t1.exon1;Parent=MS.gene049461.t1
chr3.2 CDS 56618160 56618462 56618160 ID=cds.MS.gene049461.t1;Parent=MS.gene049461.t1
chr3.2 exon 56618873 56618944 56618873 ID=MS.gene049461.t1.exon2;Parent=MS.gene049461.t1
chr3.2 CDS 56618873 56618944 56618873 ID=cds.MS.gene049461.t1;Parent=MS.gene049461.t1
Gene Sequence

>MS.gene049461

ATGGTAGAAGAAAAGAGGATCAGTGCAAATTCGAGTACCAATTCGAATTCAAACGGTGGTGGTGGGGATGAAAACGGTGATGAAGTGAAGAGTTCATGGAGTAAAAACGAAGATGAAACATTGGTGAAACTGGTGAAGGAAAACGGTGCAAGAAACTGGTCTGTGATAAGCAACGGAATCCCCGATCGTTCTGGGAAGTTTTGCCGTCTGCGGTGGTGTAATCAGCTGAGTCCAACCGTTCAACACCTTCCTTTTACACCGGCGGTGGTGGCACAACAATCATCGACGAGAATTACTGAATCTGTGCACCAAGATTCTTCGATTTTTTGGAATGGGAGTCTGATTCAGAGTGAAATTCGGGAAGAAGAAGAAGAAGTGACGGTGGAGGGAGAAAGGACTGCCGCAAACCAGAAGAAGGAAAGAAGAAGAAGAAGAGAAGAAGGAGTGCTACGTGGGCCTGGGCCTTGCCTTACAACATTGCTGATTTTTGCTACCTGCACCCTCCTTCTTGCCAAGTAGGTTTCCGTTTGCCTCAAATTGCTTGGTAAAGCTTCTAAAATTCTTCTAATTGTTTTTCTTGATAGCTTTGTTAAAGTCTTGGTGTGTTTTACGTGTTAGAATCGCTTAGGAAAATATATGTGTAAAGTGTAATTGGTATTGTTACTTTTTTTTTTTTTAATTCTTGATAAATGTTTTTTTTTGTTAAAATGTAGGTAAAATTTCCATATGTTTTGGTTCCTTTTGATCGTCCCTTGGCCATTTTTAGGATTGAATCTCGTTCTTGA

Protein sequence

>MS.gene049461.t1

MVEEKRISANSSTNSNSNGGGGDENGDEVKSSWSKNEDETLVKLVKENGARNWSVISNGIPDRSGKFCRLRWCNQLSPTVQHLPFTPAVVAQQSSTRITESVKFPYVLVPFDRPLAIFRIESRS