Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049518.t1 | XP_024642265.1 | 93.5 | 263 | 17 | 0 | 1 | 263 | 1 | 263 | 3.80E-136 | 494.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049518.t1 | A0A072TE13 | 92.8 | 263 | 19 | 0 | 1 | 263 | 1 | 263 | 3.6e-136 | 493.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene049518.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049518.t1 | MTR_1g083640 | 89.928 | 278 | 13 | 1 | 1 | 263 | 1 | 278 | 0.0 | 513 |
| MS.gene049518.t1 | MTR_0558s0020 | 92.776 | 263 | 19 | 0 | 1 | 263 | 1 | 263 | 0.0 | 511 |
| MS.gene049518.t1 | MTR_2g014100 | 86.531 | 245 | 25 | 1 | 1 | 237 | 1 | 245 | 7.57e-156 | 434 |
| MS.gene049518.t1 | MTR_8g446020 | 78.327 | 263 | 17 | 2 | 1 | 263 | 1 | 223 | 1.08e-143 | 403 |
| MS.gene049518.t1 | MTR_6g090505 | 91.414 | 198 | 17 | 0 | 66 | 263 | 1 | 198 | 2.74e-133 | 381 |
| MS.gene049518.t1 | MTR_3g008240 | 88.670 | 203 | 23 | 0 | 1 | 203 | 1 | 203 | 8.94e-133 | 374 |
| MS.gene049518.t1 | MTR_0337s0040 | 72.308 | 260 | 33 | 1 | 1 | 260 | 1 | 221 | 4.56e-132 | 373 |
| MS.gene049518.t1 | MTR_4g040430 | 88.670 | 203 | 18 | 2 | 1 | 203 | 1 | 198 | 9.80e-130 | 366 |
| MS.gene049518.t1 | MTR_5g071590 | 83.333 | 180 | 18 | 1 | 1 | 168 | 1 | 180 | 2.11e-108 | 311 |
| MS.gene049518.t1 | MTR_0019s0290 | 90.850 | 153 | 14 | 0 | 82 | 234 | 8 | 160 | 4.25e-101 | 292 |
| MS.gene049518.t1 | MTR_1g055240 | 92.308 | 143 | 11 | 0 | 91 | 233 | 2 | 144 | 7.24e-97 | 281 |
| MS.gene049518.t1 | MTR_1281s0010 | 92.661 | 109 | 8 | 0 | 20 | 128 | 1 | 109 | 3.51e-70 | 212 |
| MS.gene049518.t1 | MTR_4g008820 | 88.750 | 80 | 9 | 0 | 1 | 80 | 125 | 204 | 9.57e-40 | 146 |
| MS.gene049518.t1 | MTR_8g046060 | 82.955 | 88 | 7 | 2 | 1 | 80 | 91 | 178 | 1.50e-38 | 134 |
| MS.gene049518.t1 | MTR_6g051425 | 26.339 | 224 | 148 | 5 | 41 | 263 | 22 | 229 | 4.45e-17 | 80.1 |
| MS.gene049518.t1 | MTR_0402s0040 | 25.405 | 185 | 119 | 4 | 50 | 234 | 40 | 205 | 1.45e-13 | 70.5 |
| MS.gene049518.t1 | MTR_1g013060 | 29.032 | 217 | 133 | 9 | 41 | 254 | 29 | 227 | 1.49e-13 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049518.t1 | AT4G38180 | 26.068 | 234 | 146 | 7 | 35 | 263 | 72 | 283 | 8.94e-15 | 74.3 |
Find 39 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTTATGGGTCAAGGATT+TGG | 0.277051 | 3.2:-57203550 | MS.gene049518:CDS |
| TTTCAATCTATTAAAGAGAT+TGG | 0.308439 | 3.2:-57204006 | MS.gene049518:CDS |
| TCTGATGTTGGCCTGTTGTT+AGG | 0.329765 | 3.2:+57203829 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 3.2:-57203563 | MS.gene049518:CDS |
| GCTCTAATAAATTCTAAAGT+TGG | 0.394745 | 3.2:-57203595 | MS.gene049518:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 3.2:-57203625 | MS.gene049518:CDS |
| ATGGGTATAAGATATATAGA+TGG | 0.430188 | 3.2:-57203708 | MS.gene049518:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 3.2:-57203760 | MS.gene049518:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 3.2:-57203482 | MS.gene049518:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 3.2:-57203562 | MS.gene049518:CDS |
| TCCATGATTCGTTATTGCTA+GGG | 0.479627 | 3.2:+57204040 | None:intergenic |
| TTTAAGTCTGAAAACCGAAA+AGG | 0.483010 | 3.2:+57203511 | None:intergenic |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | 3.2:-57203840 | MS.gene049518:CDS |
| TTACTTCTCGAAATGATCTC+AGG | 0.488412 | 3.2:+57203638 | None:intergenic |
| CCCCTAGCAATAACGAATCA+TGG | 0.494071 | 3.2:-57204041 | MS.gene049518:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 3.2:-57203877 | MS.gene049518:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 3.2:+57203655 | None:intergenic |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 3.2:-57203743 | MS.gene049518:CDS |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 3.2:+57203766 | None:intergenic |
| TTCACAAACTCAATAACAAG+TGG | 0.508337 | 3.2:+57203802 | None:intergenic |
| TTCCATGATTCGTTATTGCT+AGG | 0.509536 | 3.2:+57204039 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 3.2:-57203866 | MS.gene049518:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.514358 | 3.2:+57203667 | None:intergenic |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 3.2:-57203973 | MS.gene049518:CDS |
| TGGGTATAAGATATATAGAT+GGG | 0.523615 | 3.2:-57203707 | MS.gene049518:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 3.2:-57203911 | MS.gene049518:CDS |
| TGTCTTCCGCAGTATCAACT+TGG | 0.544233 | 3.2:+57203377 | None:intergenic |
| TCTTATGAAGTTATGGGTCA+AGG | 0.549558 | 3.2:-57203556 | MS.gene049518:CDS |
| GTTATGGGTCAAGGATTTGG+AGG | 0.554514 | 3.2:-57203547 | MS.gene049518:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 3.2:-57203865 | MS.gene049518:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 3.2:-57203469 | MS.gene049518:CDS |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 3.2:-57203476 | MS.gene049518:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 3.2:-57203701 | MS.gene049518:CDS |
| CCATGATTCGTTATTGCTAG+GGG | 0.572877 | 3.2:+57204041 | None:intergenic |
| ATATCAACTTCATTTGAGAG+TGG | 0.601371 | 3.2:-57204120 | MS.gene049518:CDS |
| ACAAGTGGATTGTCTGATGT+TGG | 0.615496 | 3.2:+57203817 | None:intergenic |
| GGGTCAAGGATTTGGAGGCA+CGG | 0.631027 | 3.2:-57203542 | MS.gene049518:CDS |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 3.2:-57203473 | MS.gene049518:CDS |
| TGACATCCAAGTTGATACTG+CGG | 0.720725 | 3.2:-57203383 | MS.gene049518:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | - | chr3.2:57203560-57203579 | MS.gene049518:CDS | 20.0% |
| !! | TTTCAATCTATTAAAGAGAT+TGG | - | chr3.2:57203465-57203484 | MS.gene049518:CDS | 20.0% |
| ! | ATGGGTATAAGATATATAGA+TGG | - | chr3.2:57203763-57203782 | MS.gene049518:CDS | 25.0% |
| ! | TGGGTATAAGATATATAGAT+GGG | - | chr3.2:57203764-57203783 | MS.gene049518:CDS | 25.0% |
| !! | AAAGAGATTGGAGATTTTTA+TGG | - | chr3.2:57203477-57203496 | MS.gene049518:CDS | 25.0% |
| !! | ATAACACTGGATTTTCTATT+AGG | - | chr3.2:57203511-57203530 | MS.gene049518:CDS | 25.0% |
| !!! | GCTCTAATAAATTCTAAAGT+TGG | - | chr3.2:57203876-57203895 | MS.gene049518:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr3.2:57203606-57203625 | MS.gene049518:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr3.2:57203909-57203928 | MS.gene049518:CDS | 30.0% | |
| ATATCAACTTCATTTGAGAG+TGG | - | chr3.2:57203351-57203370 | MS.gene049518:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | chr3.2:57203594-57203613 | MS.gene049518:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | chr3.2:57203498-57203517 | MS.gene049518:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr3.2:57203605-57203624 | MS.gene049518:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr3.2:57203770-57203789 | MS.gene049518:CDS | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | - | chr3.2:57203989-57204008 | MS.gene049518:CDS | 30.0% | |
| TGGATGTCATAGTAAAAAGA+TGG | + | chr3.2:57204077-57204096 | None:intergenic | 30.0% | |
| TTCACAAACTCAATAACAAG+TGG | + | chr3.2:57203672-57203691 | None:intergenic | 30.0% | |
| TTTAAGTCTGAAAACCGAAA+AGG | + | chr3.2:57203963-57203982 | None:intergenic | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr3.2:57203807-57203826 | None:intergenic | 30.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr3.2:57203846-57203865 | MS.gene049518:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | - | chr3.2:57203695-57203714 | MS.gene049518:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | chr3.2:57203689-57203708 | MS.gene049518:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr3.2:57203908-57203927 | MS.gene049518:CDS | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | chr3.2:57203631-57203650 | MS.gene049518:CDS | 35.0% | |
| TCCATGATTCGTTATTGCTA+GGG | + | chr3.2:57203434-57203453 | None:intergenic | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | chr3.2:57203915-57203934 | MS.gene049518:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | chr3.2:57203694-57203713 | MS.gene049518:CDS | 35.0% | |
| TTACTTCTCGAAATGATCTC+AGG | + | chr3.2:57203836-57203855 | None:intergenic | 35.0% | |
| TTCCATGATTCGTTATTGCT+AGG | + | chr3.2:57203435-57203454 | None:intergenic | 35.0% | |
| !!! | AAGCTGGATGAAGTTTTGAA+TGG | - | chr3.2:57203744-57203763 | MS.gene049518:CDS | 35.0% |
| !!! | AGCTGGATGAAGTTTTGAAT+GGG | - | chr3.2:57203745-57203764 | MS.gene049518:CDS | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | - | chr3.2:57203998-57204017 | MS.gene049518:CDS | 40.0% | |
| CCATGATTCGTTATTGCTAG+GGG | + | chr3.2:57203433-57203452 | None:intergenic | 40.0% | |
| GAAGTTATGGGTCAAGGATT+TGG | - | chr3.2:57203921-57203940 | MS.gene049518:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | - | chr3.2:57203995-57204014 | MS.gene049518:CDS | 40.0% | |
| !! | ACAAGTGGATTGTCTGATGT+TGG | + | chr3.2:57203657-57203676 | None:intergenic | 40.0% |
| !! | AGCTGGAATATCTTTGAAGC+TGG | - | chr3.2:57203728-57203747 | MS.gene049518:CDS | 40.0% |
| !! | TGACATCCAAGTTGATACTG+CGG | - | chr3.2:57204088-57204107 | MS.gene049518:CDS | 40.0% |
| CCCCTAGCAATAACGAATCA+TGG | - | chr3.2:57203430-57203449 | MS.gene049518:CDS | 45.0% | |
| CTCAGGTAACACAAATGCTC+TGG | + | chr3.2:57203819-57203838 | None:intergenic | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | - | chr3.2:57203699-57203718 | MS.gene049518:CDS | 45.0% | |
| GTTATGGGTCAAGGATTTGG+AGG | - | chr3.2:57203924-57203943 | MS.gene049518:CDS | 45.0% | |
| TGTCTTCCGCAGTATCAACT+TGG | + | chr3.2:57204097-57204116 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | + | chr3.2:57203708-57203727 | None:intergenic | 45.0% | |
| ! | GCACGGAGAATCTTCCTTTT+CGG | - | chr3.2:57203946-57203965 | MS.gene049518:CDS | 45.0% |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | + | chr3.2:57203645-57203664 | None:intergenic | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr3.2:57203711-57203730 | MS.gene049518:CDS | 45.0% |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | - | chr3.2:57204002-57204021 | MS.gene049518:CDS | 50.0% |
| GGGTCAAGGATTTGGAGGCA+CGG | - | chr3.2:57203929-57203948 | MS.gene049518:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 57203342 | 57204151 | 57203342 | ID=MS.gene049518 |
| chr3.2 | mRNA | 57203342 | 57204151 | 57203342 | ID=MS.gene049518.t1;Parent=MS.gene049518 |
| chr3.2 | exon | 57203342 | 57204151 | 57203342 | ID=MS.gene049518.t1.exon1;Parent=MS.gene049518.t1 |
| chr3.2 | CDS | 57203342 | 57204151 | 57203342 | ID=cds.MS.gene049518.t1;Parent=MS.gene049518.t1 |
| Gene Sequence |
| Protein sequence |
