AlfalfaGEDB | MS.gene049542

M. sativa cultivar XinJiangDaYe / MS.gene049542

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049542.t1	PNX98523.1	70.2	161	48	0	1	161	1	161	1.30E-54	222.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049542.t1	Q9FJK3	42.9	154	88	0	1	154	1	154	4.4e-27	122.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049542.t1	A0A2K3N650	70.2	161	48	0	1	161	1	161	9.4e-55	222.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene049542.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049542.t1	MTR_2g035580	67.722	158	51	0	1	158	1	158	4.23e-64	194
MS.gene049542.t1	MTR_3g467080	50.311	161	79	1	1	160	1	161	1.08e-48	155
MS.gene049542.t1	MTR_3g466980	45.625	160	87	0	1	160	1	160	1.60e-45	149
MS.gene049542.t1	MTR_4g063790	46.626	163	86	1	1	162	1	163	3.93e-45	146
MS.gene049542.t1	MTR_3g031100	46.012	163	87	1	1	162	1	163	5.96e-40	135
MS.gene049542.t1	MTR_2g016210	46.386	166	82	3	1	161	1	164	2.10e-39	134
MS.gene049542.t1	MTR_5g075380	35.948	153	98	0	1	153	1	153	5.17e-36	122
MS.gene049542.t1	MTR_3g065100	44.156	154	86	0	1	154	1	154	1.07e-35	124
MS.gene049542.t1	MTR_3g465410	47.015	134	70	1	29	161	1	134	1.17e-35	121
MS.gene049542.t1	MTR_4g032620	36.364	154	97	1	1	153	1	154	1.65e-32	115
MS.gene049542.t1	MTR_4g031910	41.290	155	90	1	1	154	1	155	1.91e-31	113
MS.gene049542.t1	MTR_3g031240	39.264	163	96	2	1	160	1	163	1.79e-30	109
MS.gene049542.t1	MTR_3g466830	38.356	146	90	0	7	152	6	151	6.30e-30	107
MS.gene049542.t1	MTR_8g036130	38.854	157	93	2	1	154	1	157	9.93e-29	106
MS.gene049542.t1	MTR_1g090697	35.256	156	100	1	1	156	1	155	2.47e-26	97.8
MS.gene049542.t1	MTR_5g047580	35.897	156	99	1	1	156	1	155	2.50e-26	97.8
MS.gene049542.t1	MTR_1g077390	35.294	153	98	1	1	153	1	152	1.00e-25	96.3
MS.gene049542.t1	MTR_4g032260	38.065	155	95	1	1	154	1	155	3.35e-25	95.1
MS.gene049542.t1	MTR_4g028720	38.562	153	93	1	1	153	1	152	2.29e-24	92.8
MS.gene049542.t1	MTR_7g011950	37.255	153	95	1	1	153	1	152	4.15e-24	92.4
MS.gene049542.t1	MTR_2g035610	38.017	121	75	0	1	121	1	121	6.87e-24	90.5
MS.gene049542.t1	MTR_4g032290	38.217	157	96	1	7	162	7	163	8.15e-24	93.2
MS.gene049542.t1	MTR_1g090783	33.553	152	100	1	7	158	6	156	8.89e-24	91.3
MS.gene049542.t1	MTR_1g077320	34.641	153	99	1	1	153	1	152	1.97e-22	87.8
MS.gene049542.t1	MTR_1g090710	34.615	156	98	3	1	154	1	154	7.97e-22	86.3
MS.gene049542.t1	MTR_1g084950	37.662	154	95	1	1	154	1	153	9.75e-22	85.9
MS.gene049542.t1	MTR_3g466890	32.168	143	97	0	7	149	6	148	8.34e-21	84.0
MS.gene049542.t1	MTR_3g466930	35.938	128	70	3	32	153	2	123	3.00e-15	68.6
MS.gene049542.t1	MTR_3g466900	31.148	122	84	0	32	153	2	123	4.17e-15	68.2
MS.gene049542.t1	MTR_1g077300	33.750	160	103	2	1	160	1	157	6.85e-15	68.2
MS.gene049542.t1	MTR_4g028800	37.143	70	42	1	1	70	1	68	5.67e-13	61.2
MS.gene049542.t1	MTR_5g047560	27.922	154	94	2	1	154	1	137	2.10e-12	61.2
MS.gene049542.t1	MTR_6g018920	34.579	107	60	4	8	105	8	113	5.64e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049542.t1	AT5G48670	43.226	155	86	2	1	154	1	154	1.41e-30	113
MS.gene049542.t1	AT1G65330	40.385	156	91	2	7	161	6	160	2.65e-30	111
MS.gene049542.t1	AT3G05860	36.913	149	94	0	1	149	1	149	1.90e-28	104
MS.gene049542.t1	AT3G05860	36.913	149	94	0	1	149	1	149	6.72e-28	104
MS.gene049542.t1	AT3G05860	36.913	149	94	0	1	149	1	149	7.92e-28	104
MS.gene049542.t1	AT1G65300	35.948	153	98	0	4	156	3	155	5.31e-25	97.4
MS.gene049542.t1	AT1G31640	37.975	158	83	6	6	156	5	154	7.79e-22	91.3
MS.gene049542.t1	AT5G27810	42.697	89	51	0	29	117	1	89	2.21e-21	84.0
MS.gene049542.t1	AT5G27960	31.613	155	106	0	7	161	5	159	6.82e-19	82.0
MS.gene049542.t1	AT5G26650	31.613	155	106	0	7	161	5	159	1.10e-18	81.6
MS.gene049542.t1	AT1G31630	33.094	139	86	2	7	142	6	140	1.60e-18	80.9
MS.gene049542.t1	AT2G28700	34.951	103	67	0	1	103	1	103	2.07e-18	80.5
MS.gene049542.t1	AT1G22590	30.488	164	99	3	1	158	1	155	1.57e-17	75.5
MS.gene049542.t1	AT5G26630	30.435	161	112	0	1	161	1	161	5.41e-17	75.1
MS.gene049542.t1	AT5G06500	30.000	130	83	2	1	126	1	126	5.55e-15	70.1
MS.gene049542.t1	AT2G40210	25.478	157	116	1	1	157	1	156	1.98e-12	64.3
MS.gene049542.t1	AT5G26580	25.478	157	103	1	1	157	1	143	2.96e-11	60.8

Find 28 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCATTCATTTCATTCTTCT+TGG	0.187611	3.2:+57761843	None:intergenic
GAAAGGCAACATTTGGAAAA+AGG	0.291917	3.2:-57762320	MS.gene049542:CDS
TTCTTTGTTCGACTTCCTTA+AGG	0.340784	3.2:+57761731	None:intergenic
TTTGTTTCTTTAACTTCTCT+AGG	0.340953	3.2:+57761875	None:intergenic
AATGAAATGAATGATCTAAT+TGG	0.349404	3.2:-57761835	MS.gene049542:CDS
ATTCATTCATACGGGTGAAT+TGG	0.359950	3.2:-57761809	MS.gene049542:CDS
TCGAAGAGAAAGGCAACATT+TGG	0.402575	3.2:-57762327	MS.gene049542:CDS
TGAACTTGTCCAACACCATA+TGG	0.431567	3.2:+57761974	None:intergenic
TTGTTTCTTTAACTTCTCTA+GGG	0.453550	3.2:+57761876	None:intergenic
AATTGGTCAATTCATTCATA+CGG	0.459890	3.2:-57761818	MS.gene049542:CDS
AATGAAATCACCACCTTATG+TGG	0.471722	3.2:-57762069	MS.gene049542:CDS
GCTTATTGTTGAGAACCTTA+AGG	0.479647	3.2:-57761746	MS.gene049542:CDS
ACATACATCAATTCCACATA+AGG	0.491397	3.2:+57762056	None:intergenic
ATTCATACGGGTGAATTGGA+TGG	0.523018	3.2:-57761805	MS.gene049542:CDS
CTTCGAATTTCTAGCAGCCA+TGG	0.529100	3.2:+57762371	None:intergenic
AATTGATGACTCGAAGAGAA+AGG	0.532922	3.2:-57762337	MS.gene049542:CDS
AAAATCGATGGTGGATCATG+AGG	0.544806	3.2:-57761926	MS.gene049542:CDS
ATTGGTCAATTCATTCATAC+GGG	0.554460	3.2:-57761817	MS.gene049542:CDS
GGTGAATTGGATGGAAATGT+TGG	0.586534	3.2:-57761796	MS.gene049542:CDS
AAGTCGAACAAAGAATCGAA+TGG	0.588974	3.2:-57761723	MS.gene049542:CDS
AAATCGATGGTGGATCATGA+GGG	0.613825	3.2:-57761925	MS.gene049542:CDS
TGTATGTGGTATAATCTATG+AGG	0.618109	3.2:-57762040	MS.gene049542:CDS
GGAAAACAACGCTCAAGCAA+TGG	0.621665	3.2:-57762019	MS.gene049542:CDS
TACATCAATTCCACATAAGG+TGG	0.624843	3.2:+57762059	None:intergenic
ATATGAACGAAGAAAATCGA+TGG	0.638659	3.2:-57761938	MS.gene049542:CDS
TGAACGAAGAAAATCGATGG+TGG	0.640332	3.2:-57761935	MS.gene049542:CDS
TTATGTGGAATTGATGTATG+TGG	0.644295	3.2:-57762054	MS.gene049542:CDS
TGGATCATGAGGGCTTCTTG+AGG	0.670340	3.2:-57761915	MS.gene049542:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTGCTTCATAAAAATTA+AGG	+	chr3.2:57761799-57761818	None:intergenic	15.0%
!!	AATGAAATGAATGATCTAAT+TGG	-	chr3.2:57762233-57762252	MS.gene049542:intron	20.0%
!!	ACTTTATACTATAAATGACA+TGG	+	chr3.2:57761916-57761935	None:intergenic	20.0%
!	AATTGGTCAATTCATTCATA+CGG	-	chr3.2:57762250-57762269	MS.gene049542:intron	25.0%
!	ATCATTCATTTCATTCTTCT+TGG	+	chr3.2:57762228-57762247	None:intergenic	25.0%
!	CATACTGATGTTCATTATAA+TGG	+	chr3.2:57761839-57761858	None:intergenic	25.0%
!!	TTGTTTCTTTAACTTCTCTA+GGG	+	chr3.2:57762195-57762214	None:intergenic	25.0%
!!	TTTGTTTCTTTAACTTCTCT+AGG	+	chr3.2:57762196-57762215	None:intergenic	25.0%
	ACATACATCAATTCCACATA+AGG	+	chr3.2:57762015-57762034	None:intergenic	30.0%
	ACATTTGGAAAAAGGAAACA+AGG	-	chr3.2:57761756-57761775	MS.gene049542:CDS	30.0%
	AGTATGCATCGTTTGATATT+AGG	-	chr3.2:57761853-57761872	MS.gene049542:CDS	30.0%
	ATATGAACGAAGAAAATCGA+TGG	-	chr3.2:57762130-57762149	MS.gene049542:intron	30.0%
	ATCCAAAGAAAAAGTCCATA+TGG	-	chr3.2:57762079-57762098	MS.gene049542:CDS	30.0%
	ATTGGTCAATTCATTCATAC+GGG	-	chr3.2:57762251-57762270	MS.gene049542:intron	30.0%
	TGTATGTGGTATAATCTATG+AGG	-	chr3.2:57762028-57762047	MS.gene049542:CDS	30.0%
	TTATGTGGAATTGATGTATG+TGG	-	chr3.2:57762014-57762033	MS.gene049542:CDS	30.0%
!	GATTTTCTTCGTTCATATTC+AGG	+	chr3.2:57762128-57762147	None:intergenic	30.0%
!	TCAGTTTTACATTTCTCTGA+CGG	-	chr3.2:57761937-57761956	MS.gene049542:CDS	30.0%
!!	ATATGGACTTTTTCTTTGGA+TGG	+	chr3.2:57762080-57762099	None:intergenic	30.0%
!!	CTCTTGCTATATTTTCAAAC+AGG	-	chr3.2:57761960-57761979	MS.gene049542:CDS	30.0%
	AAGTCGAACAAAGAATCGAA+TGG	-	chr3.2:57762345-57762364	MS.gene049542:CDS	35.0%
	AATGAAATCACCACCTTATG+TGG	-	chr3.2:57761999-57762018	MS.gene049542:CDS	35.0%
	AGAAAAAGTCCATATGGTGT+TGG	-	chr3.2:57762085-57762104	MS.gene049542:CDS	35.0%
	ATTCATTCATACGGGTGAAT+TGG	-	chr3.2:57762259-57762278	MS.gene049542:intron	35.0%
	GAAAGGCAACATTTGGAAAA+AGG	-	chr3.2:57761748-57761767	MS.gene049542:CDS	35.0%
	GCTTATTGTTGAGAACCTTA+AGG	-	chr3.2:57762322-57762341	MS.gene049542:CDS	35.0%
	TACATCAATTCCACATAAGG+TGG	+	chr3.2:57762012-57762031	None:intergenic	35.0%
	TTCTTTGTTCGACTTCCTTA+AGG	+	chr3.2:57762340-57762359	None:intergenic	35.0%
!	AATTGATGACTCGAAGAGAA+AGG	-	chr3.2:57761731-57761750	MS.gene049542:CDS	35.0%
!!	CACCATATGGACTTTTTCTT+TGG	+	chr3.2:57762084-57762103	None:intergenic	35.0%
	ATTCATACGGGTGAATTGGA+TGG	-	chr3.2:57762263-57762282	MS.gene049542:intron	40.0%
	GGTGAATTGGATGGAAATGT+TGG	-	chr3.2:57762272-57762291	MS.gene049542:intron	40.0%
	TCGAAGAGAAAGGCAACATT+TGG	-	chr3.2:57761741-57761760	MS.gene049542:CDS	40.0%
	TGAACGAAGAAAATCGATGG+TGG	-	chr3.2:57762133-57762152	MS.gene049542:intron	40.0%
	TGAACTTGTCCAACACCATA+TGG	+	chr3.2:57762097-57762116	None:intergenic	40.0%
!!	AAAATCGATGGTGGATCATG+AGG	-	chr3.2:57762142-57762161	MS.gene049542:intron	40.0%
!!	AAATCGATGGTGGATCATGA+GGG	-	chr3.2:57762143-57762162	MS.gene049542:intron	40.0%
	GGAAAACAACGCTCAAGCAA+TGG	-	chr3.2:57762049-57762068	MS.gene049542:CDS	45.0%
!!!	CTTCTCTAGGGCTTTTGCTA+TGG	+	chr3.2:57762183-57762202	None:intergenic	45.0%
	TGGATCATGAGGGCTTCTTG+AGG	-	chr3.2:57762153-57762172	MS.gene049542:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	57761699	57762391	57761699	ID=MS.gene049542
chr3.2	mRNA	57761699	57762391	57761699	ID=MS.gene049542.t1;Parent=MS.gene049542
chr3.2	exon	57762313	57762391	57762313	ID=MS.gene049542.t1.exon1;Parent=MS.gene049542.t1
chr3.2	CDS	57762313	57762391	57762313	ID=cds.MS.gene049542.t1;Parent=MS.gene049542.t1
chr3.2	exon	57761699	57762108	57761699	ID=MS.gene049542.t1.exon2;Parent=MS.gene049542.t1
chr3.2	CDS	57761699	57762108	57761699	ID=cds.MS.gene049542.t1;Parent=MS.gene049542.t1

Gene Sequence

>MS.gene049542

ATGGCTGCTAGAAATTCGAAGATTGAATACATAATTGATGACTCGAAGAGAAAGGCAACATTTGGAAAAAGGAAACAAGGTAATATACTAGCTTTCTCCTTAATTTTTATGAAGCAAATTCATATCAAACAACTTTACCATTATAATGAACATCAGTATGCATCGTTTGATATTAGGATTTATTTATTTTATCTCTTCAGACAATAATTTAAAGCCATGTCATTTATAGTATAAAGTATCAGTTTTACATTTCTCTGACGGCTCTTGCTATATTTTCAAACAGGCATAATTAAGAAGATTAATGAAATCACCACCTTATGTGGAATTGATGTATGTGGTATAATCTATGAGGAAAACAACGCTCAAGCAATGGTTTGTCCATCCAAAGAAAAAGTCCATATGGTGTTGGACAAGTTCATGAGTTTTCCTGAATATGAACGAAGAAAATCGATGGTGGATCATGAGGGCTTCTTGAGGCAATCCATAGCAAAAGCCCTAGAGAAGTTAAAGAAACAAAAGAACAACTCCAAGAAGAATGAAATGAATGATCTAATTGGTCAATTCATTCATACGGGTGAATTGGATGGAAATGTTGGTGAGAGTGATCTTGAAGATCTGTTGTTGCTTATTGTTGAGAACCTTAAGGAAGTCGAACAAAGAATCGAATGGATTCAAGCTCAAGATGAAGATTAA

Protein sequence

>MS.gene049542.t1

MAARNSKIEYIIDDSKRKATFGKRKQGIIKKINEITTLCGIDVCGIIYEENNAQAMVCPSKEKVHMVLDKFMSFPEYERRKSMVDHEGFLRQSIAKALEKLKKQKNNSKKNEMNDLIGQFIHTGELDGNVGESDLEDLLLLIVENLKEVEQRIEWIQAQDED