AlfalfaGEDB | MS.gene049759

M. sativa cultivar XinJiangDaYe / MS.gene049759

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: PF13912.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049759.t1	XP_003606327.1	88.1	294	30	2	1	290	1	293	3.90E-118	434.5

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049759.t1	Q681X4	44.2	208	72	5	100	290	106	286	2.1e-35	151.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049759.t1	G7JN94	88.1	294	30	2	1	290	1	293	2.8e-118	434.5

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene049759.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049759.t1	MTR_4g057230	92.517	294	17	2	1	290	1	293	3.85e-168	468
MS.gene049759.t1	MTR_2g084315	45.860	314	95	12	17	290	15	293	7.25e-69	216
MS.gene049759.t1	MTR_1g090703	38.172	372	116	13	8	290	9	355	1.04e-60	196
MS.gene049759.t1	MTR_1g062780	37.931	319	141	12	5	290	3	297	1.78e-50	168
MS.gene049759.t1	MTR_1g067650	33.784	222	65	7	55	264	5	156	1.29e-25	100
MS.gene049759.t1	MTR_1g067640	38.690	168	41	5	109	264	38	155	2.33e-25	99.8
MS.gene049759.t1	MTR_7g023560	35.227	176	43	4	62	234	8	115	2.93e-23	94.4
MS.gene049759.t1	MTR_1g115345	33.889	180	60	5	56	235	6	126	2.00e-22	92.8
MS.gene049759.t1	MTR_7g100100	37.267	161	47	5	109	262	34	147	6.23e-21	87.8
MS.gene049759.t1	MTR_1g077730	40.000	160	78	6	108	258	43	193	4.67e-20	86.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049759.t1	AT2G28200	42.718	309	109	11	17	290	11	286	8.98e-57	184
MS.gene049759.t1	AT5G03510	39.803	304	121	12	21	290	17	292	3.28e-52	173
MS.gene049759.t1	AT5G04390	33.598	378	128	11	14	290	7	362	1.07e-48	166
MS.gene049759.t1	AT3G10470	32.166	314	113	7	60	290	102	398	1.41e-39	142
MS.gene049759.t1	AT2G37430	33.645	214	72	5	58	263	9	160	2.03e-27	105
MS.gene049759.t1	AT3G53600	28.959	221	80	6	53	263	4	157	3.18e-20	86.3
MS.gene049759.t1	AT3G60580	34.722	216	81	7	63	269	124	288	1.27e-18	84.3
MS.gene049759.t1	AT1G02030	33.974	156	45	5	109	262	160	259	4.81e-15	73.9
MS.gene049759.t1	AT3G19580	29.885	174	73	6	56	227	61	187	8.77e-15	73.2
MS.gene049759.t1	AT3G19580	29.885	174	73	6	56	227	61	187	8.77e-15	73.2

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Find 92 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGGGAAAAGGGTAGTCA+TGG	0.501331	4.2:+46550100	MS.gene049759:CDS
AAAGAAACCATGGGCCCACG+AGG	0.682791	4.2:-46550376	None:intergenic
AAAGCCTAAAATTATGGCTG+AGG	0.550901	4.2:+46550240	MS.gene049759:CDS
AACCATGATGATTCCCCATT+TGG	0.275629	4.2:-46550399	None:intergenic
AAGAAGCTGAGAAACCTCGT+GGG	0.704144	4.2:+46550362	MS.gene049759:CDS
AAGCATTGGGTGGACACATG+AGG	0.635825	4.2:+46550497	MS.gene049759:CDS
AATGGAACACTCATGAATCT+TGG	0.473930	4.2:-46550449	None:intergenic
AATGGTGATGGCTGCTGCTC+CGG	0.446387	4.2:+46550672	MS.gene049759:CDS
ACAATTGGATCTTAACCTTC+CGG	0.387432	4.2:+46550594	MS.gene049759:CDS
ACATCCGGACAAGCATTGGG+TGG	0.645911	4.2:+46550487	MS.gene049759:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGACAAAAATCTTAACCTCT+TGG	-	chr4.2:46549927-46549946	None:intergenic	30.0%
GATGAGAATAAAAGGGAAAA+GGG	+	chr4.2:46550091-46550110	MS.gene049759:CDS	30.0%
GTTTGAAAGCAGCTATTAAT+GGG	+	chr4.2:46550419-46550438	MS.gene049759:CDS	30.0%
TGATGAGAATAAAAGGGAAA+AGG	+	chr4.2:46550090-46550109	MS.gene049759:CDS	30.0%
AAAGCCTAAAATTATGGCTG+AGG	+	chr4.2:46550240-46550259	MS.gene049759:CDS	35.0%
AATGGAACACTCATGAATCT+TGG	-	chr4.2:46550452-46550471	None:intergenic	35.0%
ACAATTGGATCTTAACCTTC+CGG	+	chr4.2:46550594-46550613	MS.gene049759:CDS	35.0%
ATTCATGAGTGTTCCATTTG+TGG	+	chr4.2:46550454-46550473	MS.gene049759:CDS	35.0%
CTTGAAAGGAAGGAAAAGTT+CGG	-	chr4.2:46550192-46550211	None:intergenic	35.0%
GGAAACAAAGTTTCAGTTTC+CGG	+	chr4.2:46550636-46550655	MS.gene049759:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	46549845	46550717	46549845	ID=MS.gene049759
chr4.2	mRNA	46549845	46550717	46549845	ID=MS.gene049759.t1;Parent=MS.gene049759
chr4.2	exon	46549845	46550717	46549845	ID=MS.gene049759.t1.exon1;Parent=MS.gene049759.t1
chr4.2	CDS	46549845	46550717	46549845	ID=cds.MS.gene049759.t1;Parent=MS.gene049759.t1

Gene Sequence

>MS.gene049759

ATGGAAGAGTTTGTTATGGAGGAAAAGATGGTGAGTTATGCTAAGAAAGATGCAACTCACATAGCCAAAGGAAAACGTACCAAGAGGTTAAGATTTTTGTCTCCTTGTGGTGTTGTTGCTATGGCATCTAGTTGTTCAAGTGATGAGAGCAACATTGATCTTGAAGAAGATGAAGAAGATGATGATGAAGAAGATTTGGCTAAGTGTTTGATTCTTCTAGCTCAAGGTGGAAATCATAGGCTTGTTGATGAGAATAAAAGGGAAAAGGGTAGTCATGGAAATAAAAAAATTGGTGAGACTAGCACAAAACTTGGTTTGTATATTTATGAATGCAAAACATGTAACCGAACTTTTCCTTCCTTTCAAGCTTTGGGTGGTCACAGGGCAAGTCACAAAAAGCCTAAAATTATGGCTGAGGAGAAGAAACCATCATCACCGCCGCAGCAGCAGCCTCGGCCACAGTCTTCATCACATGATTCGCAATCAGTTAACCTGGTTGCGAATAGTGATGAGTTTGAAGAAGCTGAGAAACCTCGTGGGCCCATGGTTTCTTTCCAAATGGGGAATCATCATGGTTTGAAAGCAGCTATTAATGGGAACAAGGCCAAGATTCATGAGTGTTCCATTTGTGGTTCAGAGTTCACATCCGGACAAGCATTGGGTGGACACATGAGGAGGCATAGGGCATCAGTGGCAAACGCCGCGGCTGTCGCGGCCCCGGATGAAGGAGTTAGGCCAAGGAATATTTTACAATTGGATCTTAACCTTCCGGCACCGGAGGAGGATATCCGGGAAACAAAGTTTCAGTTTCCGGCTACACAGAAATCAATGGTGATGGCTGCTGCTCCGGCTTTGGTGGGTTGCCATTATTAG

Protein sequence

>MS.gene049759.t1

MEEFVMEEKMVSYAKKDATHIAKGKRTKRLRFLSPCGVVAMASSCSSDESNIDLEEDEEDDDEEDLAKCLILLAQGGNHRLVDENKREKGSHGNKKIGETSTKLGLYIYECKTCNRTFPSFQALGGHRASHKKPKIMAEEKKPSSPPQQQPRPQSSSHDSQSVNLVANSDEFEEAEKPRGPMVSFQMGNHHGLKAAINGNKAKIHECSICGSEFTSGQALGGHMRRHRASVANAAAVAAPDEGVRPRNILQLDLNLPAPEEDIRETKFQFPATQKSMVMAAAPALVGCHY