AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049759

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049759.t1 MTR_4g057230 92.517 294 17 2 1 290 1 293 3.85e-168 468
MS.gene049759.t1 MTR_2g084315 45.860 314 95 12 17 290 15 293 7.25e-69 216
MS.gene049759.t1 MTR_1g090703 38.172 372 116 13 8 290 9 355 1.04e-60 196
MS.gene049759.t1 MTR_1g062780 37.931 319 141 12 5 290 3 297 1.78e-50 168
MS.gene049759.t1 MTR_1g067650 33.784 222 65 7 55 264 5 156 1.29e-25 100
MS.gene049759.t1 MTR_1g067640 38.690 168 41 5 109 264 38 155 2.33e-25 99.8
MS.gene049759.t1 MTR_7g023560 35.227 176 43 4 62 234 8 115 2.93e-23 94.4
MS.gene049759.t1 MTR_1g115345 33.889 180 60 5 56 235 6 126 2.00e-22 92.8
MS.gene049759.t1 MTR_7g100100 37.267 161 47 5 109 262 34 147 6.23e-21 87.8
MS.gene049759.t1 MTR_1g077730 40.000 160 78 6 108 258 43 193 4.67e-20 86.3
MS.gene049759.t1 MTR_4g109340 37.342 158 80 4 109 262 348 490 3.92e-19 87.4
MS.gene049759.t1 MTR_4g028610 37.209 172 71 6 108 259 226 380 1.44e-18 85.9
MS.gene049759.t1 MTR_0160s0030 34.615 156 46 4 109 262 240 341 5.12e-18 83.6
MS.gene049759.t1 MTR_1g077700 34.426 183 78 6 85 258 36 185 2.50e-16 75.9
MS.gene049759.t1 MTR_4g027440 36.364 165 52 5 109 259 220 345 2.89e-16 78.6
MS.gene049759.t1 MTR_2g096540 31.792 173 56 6 96 266 19 131 5.54e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049759.t1 AT2G28200 42.718 309 109 11 17 290 11 286 8.98e-57 184
MS.gene049759.t1 AT5G03510 39.803 304 121 12 21 290 17 292 3.28e-52 173
MS.gene049759.t1 AT5G04390 33.598 378 128 11 14 290 7 362 1.07e-48 166
MS.gene049759.t1 AT3G10470 32.166 314 113 7 60 290 102 398 1.41e-39 142
MS.gene049759.t1 AT2G37430 33.645 214 72 5 58 263 9 160 2.03e-27 105
MS.gene049759.t1 AT3G53600 28.959 221 80 6 53 263 4 157 3.18e-20 86.3
MS.gene049759.t1 AT3G60580 34.722 216 81 7 63 269 124 288 1.27e-18 84.3
MS.gene049759.t1 AT1G02030 33.974 156 45 5 109 262 160 259 4.81e-15 73.9
MS.gene049759.t1 AT3G19580 29.885 174 73 6 56 227 61 187 8.77e-15 73.2
MS.gene049759.t1 AT3G19580 29.885 174 73 6 56 227 61 187 8.77e-15 73.2
MS.gene049759.t1 AT5G67450 30.918 207 88 7 22 227 34 186 2.68e-13 68.6

Find 92 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAACAAAGTTTCAGTTTC+CGG 0.212657 4.2:+46550636 MS.gene049759:CDS
GGCTTGTTGATGAGAATAAA+AGG 0.257462 4.2:+46550083 MS.gene049759:CDS
TTTCCTTCCTTTCAAGCTTT+GGG 0.266273 4.2:+46550196 MS.gene049759:CDS
AACCATGATGATTCCCCATT+TGG 0.275629 4.2:-46550399 None:intergenic
GCCCATGGTTTCTTTCCAAA+TGG 0.317101 4.2:+46550384 MS.gene049759:CDS
GGTTTGAAAGCAGCTATTAA+TGG 0.332072 4.2:+46550418 MS.gene049759:CDS
GATGGCTGCTGCTCCGGCTT+TGG 0.337938 4.2:+46550678 MS.gene049759:CDS
TGCTGCTGCGGCGGTGATGA+TGG 0.352089 4.2:-46550271 None:intergenic
GGCTGCTGCTCCGGCTTTGG+TGG 0.355185 4.2:+46550681 MS.gene049759:CDS
CGCCGCGGCTGTCGCGGCCC+CGG 0.362249 4.2:+46550543 MS.gene049759:CDS
AGATGATGATGAAGAAGATT+TGG 0.365412 4.2:+46550021 MS.gene049759:CDS
CCCATGGTTTCTTTCCAAAT+GGG 0.368865 4.2:+46550385 MS.gene049759:CDS
TTTGGTGGGTTGCCATTATT+AGG 0.369907 4.2:+46550696 MS.gene049759:CDS
GAAACTGAAACTTTGTTTCC+CGG 0.371224 4.2:-46550633 None:intergenic
ATCCCACCATTGCCTAATAA+TGG 0.373982 4.2:-46550708 None:intergenic
ATTCATGAGTGTTCCATTTG+TGG 0.380454 4.2:+46550454 MS.gene049759:CDS
GTTTGAAAGCAGCTATTAAT+GGG 0.383602 4.2:+46550419 MS.gene049759:CDS
ACAATTGGATCTTAACCTTC+CGG 0.387432 4.2:+46550594 MS.gene049759:CDS
CACCGCCGCAGCAGCAGCCT+CGG 0.399803 4.2:+46550278 MS.gene049759:CDS
TTCAAGCTTTGGGTGGTCAC+AGG 0.400243 4.2:+46550206 MS.gene049759:CDS
TGAGATGGAAGAGTTTGTTA+TGG 0.402618 4.2:+46549841 None:intergenic
CGGCACCGGAGGAGGATATC+CGG 0.403948 4.2:+46550614 MS.gene049759:CDS
TAGCTCAAGGTGGAAATCAT+AGG 0.408004 4.2:+46550062 MS.gene049759:CDS
CTTGGCCTAACTCCTTCATC+CGG 0.411052 4.2:-46550562 None:intergenic
TGTGAACTCTGAACCACAAA+TGG 0.422342 4.2:-46550467 None:intergenic
GCTGTCGCGGCCCCGGATGA+AGG 0.431837 4.2:+46550550 MS.gene049759:CDS
TCCTTGTGGTGTTGTTGCTA+TGG 0.433985 4.2:+46549946 MS.gene049759:CDS
GGCAAACGCCGCGGCTGTCG+CGG 0.436027 4.2:+46550537 MS.gene049759:CDS
TCCAATTGTAAAATATTCCT+TGG 0.441862 4.2:-46550580 None:intergenic
TGATGAGAATAAAAGGGAAA+AGG 0.444034 4.2:+46550090 MS.gene049759:CDS
AATGGTGATGGCTGCTGCTC+CGG 0.446387 4.2:+46550672 MS.gene049759:CDS
CGGATATCCTCCTCCGGTGC+CGG 0.447534 4.2:-46550613 None:intergenic
CGGCCCCGGATGAAGGAGTT+AGG 0.454771 4.2:+46550557 MS.gene049759:CDS
GATGAGAATAAAAGGGAAAA+GGG 0.455980 4.2:+46550091 MS.gene049759:CDS
GTGTCCACCCAATGCTTGTC+CGG 0.464924 4.2:-46550491 None:intergenic
GGCCGAGGCTGCTGCTGCGG+CGG 0.468210 4.2:-46550280 None:intergenic
ATCCGGGGCCGCGACAGCCG+CGG 0.468884 4.2:-46550545 None:intergenic
ACCATTGATTTCTGTGTAGC+CGG 0.469358 4.2:-46550655 None:intergenic
TTCCAAATGGGGAATCATCA+TGG 0.470180 4.2:+46550397 MS.gene049759:CDS
GTTTGTTATGGAGGAAAAGA+TGG 0.472339 4.2:+46549853 MS.gene049759:CDS
AATGGAACACTCATGAATCT+TGG 0.473930 4.2:-46550449 None:intergenic
TTGGCCTAACTCCTTCATCC+GGG 0.483136 4.2:-46550561 None:intergenic
GATGGAAGAGTTTGTTATGG+AGG 0.484063 4.2:+46549844 None:intergenic
GGATCTTAACCTTCCGGCAC+CGG 0.484472 4.2:+46550600 MS.gene049759:CDS
GGTGAGACTAGCACAAAACT+TGG 0.487527 4.2:+46550136 MS.gene049759:CDS
TTCACATCCGGACAAGCATT+GGG 0.492037 4.2:+46550484 MS.gene049759:CDS
TAACCTTCCGGCACCGGAGG+AGG 0.492624 4.2:+46550606 MS.gene049759:CDS
TGTGGTTCAGAGTTCACATC+CGG 0.498637 4.2:+46550472 MS.gene049759:CDS
GCTGCTGCTCCGGCTTTGGT+GGG 0.498866 4.2:+46550682 MS.gene049759:CDS
AAAAGGGAAAAGGGTAGTCA+TGG 0.501331 4.2:+46550100 MS.gene049759:CDS
GTGGACACATGAGGAGGCAT+AGG 0.504351 4.2:+46550506 MS.gene049759:CDS
CTTGAAAGGAAGGAAAAGTT+CGG 0.506003 4.2:-46550189 None:intergenic
GCTTGTTGATGAGAATAAAA+GGG 0.507642 4.2:+46550084 MS.gene049759:CDS
GTTCACATCCGGACAAGCAT+TGG 0.508676 4.2:+46550483 MS.gene049759:CDS
CCTTCCTTTCAAGCTTTGGG+TGG 0.514201 4.2:+46550199 MS.gene049759:CDS
TGTTTGATTCTTCTAGCTCA+AGG 0.518149 4.2:+46550049 MS.gene049759:CDS
GTTTCCCGGATATCCTCCTC+CGG 0.518540 4.2:-46550619 None:intergenic
GTGACCACCCAAAGCTTGAA+AGG 0.519690 4.2:-46550203 None:intergenic
TAATGGCAACCCACCAAAGC+CGG 0.522225 4.2:-46550691 None:intergenic
TGTGGCCGAGGCTGCTGCTG+CGG 0.525499 4.2:-46550283 None:intergenic
CCATGGTTTCTTTCCAAATG+GGG 0.530266 4.2:+46550386 MS.gene049759:CDS
GGCACCGGAGGAGGATATCC+GGG 0.532359 4.2:+46550615 MS.gene049759:CDS
CCACCCAAAGCTTGAAAGGA+AGG 0.534337 4.2:-46550199 None:intergenic
TCAAGCTTTGGGTGGTCACA+GGG 0.535579 4.2:+46550207 MS.gene049759:CDS
AAAGCCTAAAATTATGGCTG+AGG 0.550901 4.2:+46550240 MS.gene049759:CDS
TGAGAAACCTCGTGGGCCCA+TGG 0.552592 4.2:+46550369 MS.gene049759:CDS
TGGACACATGAGGAGGCATA+GGG 0.553507 4.2:+46550507 MS.gene049759:CDS
CCCCATTTGGAAAGAAACCA+TGG 0.566223 4.2:-46550386 None:intergenic
GAGGAGGCATAGGGCATCAG+TGG 0.587332 4.2:+46550516 MS.gene049759:CDS
TGATTCGCAATCAGTTAACC+TGG 0.588441 4.2:+46550318 MS.gene049759:CDS
ACTCATCACTATTCGCAACC+AGG 0.588455 4.2:-46550336 None:intergenic
TCCGGCTACACAGAAATCAA+TGG 0.589930 4.2:+46550654 MS.gene049759:CDS
TACACAGAAATCAATGGTGA+TGG 0.590067 4.2:+46550660 MS.gene049759:CDS
TGTGATGAAGACTGTGGCCG+AGG 0.593635 4.2:-46550295 None:intergenic
TATCCTCCTCCGGTGCCGGA+AGG 0.594929 4.2:-46550609 None:intergenic
AGCAGCTATTAATGGGAACA+AGG 0.601134 4.2:+46550426 MS.gene049759:CDS
TCTTAACCTTCCGGCACCGG+AGG 0.607477 4.2:+46550603 MS.gene049759:CDS
TGGCCTAACTCCTTCATCCG+GGG 0.608914 4.2:-46550560 None:intergenic
CGGATGAAGGAGTTAGGCCA+AGG 0.613650 4.2:+46550563 MS.gene049759:CDS
CCCATTTGGAAAGAAACCAT+GGG 0.614973 4.2:-46550385 None:intergenic
GATGCAACTCACATAGCCAA+AGG 0.631447 4.2:+46549893 MS.gene049759:CDS
AAGCATTGGGTGGACACATG+AGG 0.635825 4.2:+46550497 MS.gene049759:CDS
CATTGGGTGGACACATGAGG+AGG 0.644735 4.2:+46550500 MS.gene049759:CDS
GCCATAGCAACAACACCACA+AGG 0.645146 4.2:-46549947 None:intergenic
ACATCCGGACAAGCATTGGG+TGG 0.645911 4.2:+46550487 MS.gene049759:CDS
GGCATCAGTGGCAAACGCCG+CGG 0.646242 4.2:+46550528 MS.gene049759:CDS
TTGATTCTTCTAGCTCAAGG+TGG 0.647646 4.2:+46550052 MS.gene049759:CDS
GAAGAAGCTGAGAAACCTCG+TGG 0.659484 4.2:+46550361 MS.gene049759:CDS
CCAAAGGAAAACGTACCAAG+AGG 0.677248 4.2:+46549909 MS.gene049759:CDS
AAAGAAACCATGGGCCCACG+AGG 0.682791 4.2:-46550376 None:intergenic
AAGAAGCTGAGAAACCTCGT+GGG 0.704144 4.2:+46550362 MS.gene049759:CDS
GAATCATGTGATGAAGACTG+TGG 0.801031 4.2:-46550301 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTCATGGAAATAAAAAAAT+TGG + chr4.2:46550115-46550134 MS.gene049759:CDS 20.0%
! TCACAAAAAGCCTAAAATTA+TGG + chr4.2:46550234-46550253 MS.gene049759:CDS 25.0%
! TCCAATTGTAAAATATTCCT+TGG - chr4.2:46550583-46550602 None:intergenic 25.0%
AGACAAAAATCTTAACCTCT+TGG - chr4.2:46549927-46549946 None:intergenic 30.0%
GATGAGAATAAAAGGGAAAA+GGG + chr4.2:46550091-46550110 MS.gene049759:CDS 30.0%
GTTTGAAAGCAGCTATTAAT+GGG + chr4.2:46550419-46550438 MS.gene049759:CDS 30.0%
TGATGAGAATAAAAGGGAAA+AGG + chr4.2:46550090-46550109 MS.gene049759:CDS 30.0%
! AGATGATGATGAAGAAGATT+TGG + chr4.2:46550021-46550040 MS.gene049759:CDS 30.0%
! GCCAAGGAATATTTTACAAT+TGG + chr4.2:46550579-46550598 MS.gene049759:CDS 30.0%
! GCTTGTTGATGAGAATAAAA+GGG + chr4.2:46550084-46550103 MS.gene049759:CDS 30.0%
! TTAAGATTTTTGTCTCCTTG+TGG + chr4.2:46549932-46549951 MS.gene049759:CDS 30.0%
AAAGCCTAAAATTATGGCTG+AGG + chr4.2:46550240-46550259 MS.gene049759:CDS 35.0%
AATGGAACACTCATGAATCT+TGG - chr4.2:46550452-46550471 None:intergenic 35.0%
ACAATTGGATCTTAACCTTC+CGG + chr4.2:46550594-46550613 MS.gene049759:CDS 35.0%
ATTCATGAGTGTTCCATTTG+TGG + chr4.2:46550454-46550473 MS.gene049759:CDS 35.0%
CTTGAAAGGAAGGAAAAGTT+CGG - chr4.2:46550192-46550211 None:intergenic 35.0%
GGAAACAAAGTTTCAGTTTC+CGG + chr4.2:46550636-46550655 MS.gene049759:CDS 35.0%
GGTTTGAAAGCAGCTATTAA+TGG + chr4.2:46550418-46550437 MS.gene049759:CDS 35.0%
TACACAGAAATCAATGGTGA+TGG + chr4.2:46550660-46550679 MS.gene049759:CDS 35.0%
TTTCCTTCCTTTCAAGCTTT+GGG + chr4.2:46550196-46550215 MS.gene049759:CDS 35.0%
! GAAACTGAAACTTTGTTTCC+CGG - chr4.2:46550636-46550655 None:intergenic 35.0%
! TTCTCCTCAGCCATAATTTT+AGG - chr4.2:46550247-46550266 None:intergenic 35.0%
! TTTTCCTTCCTTTCAAGCTT+TGG + chr4.2:46550195-46550214 MS.gene049759:CDS 35.0%
!! GGCTTGTTGATGAGAATAAA+AGG + chr4.2:46550083-46550102 MS.gene049759:CDS 35.0%
!! GTTTGTTATGGAGGAAAAGA+TGG + chr4.2:46549853-46549872 MS.gene049759:CDS 35.0%
!! TGTTTGATTCTTCTAGCTCA+AGG + chr4.2:46550049-46550068 MS.gene049759:CDS 35.0%
AAAAGGGAAAAGGGTAGTCA+TGG + chr4.2:46550100-46550119 MS.gene049759:CDS 40.0%
AACCATGATGATTCCCCATT+TGG - chr4.2:46550402-46550421 None:intergenic 40.0%
AGCAGCTATTAATGGGAACA+AGG + chr4.2:46550426-46550445 MS.gene049759:CDS 40.0%
CCATGGTTTCTTTCCAAATG+GGG + chr4.2:46550386-46550405 MS.gene049759:CDS 40.0%
CCCATGGTTTCTTTCCAAAT+GGG + chr4.2:46550385-46550404 MS.gene049759:CDS 40.0%
CCCATTTGGAAAGAAACCAT+GGG - chr4.2:46550388-46550407 None:intergenic 40.0%
GAATCATGTGATGAAGACTG+TGG - chr4.2:46550304-46550323 None:intergenic 40.0%
TAGCTCAAGGTGGAAATCAT+AGG + chr4.2:46550062-46550081 MS.gene049759:CDS 40.0%
TGATTCGCAATCAGTTAACC+TGG + chr4.2:46550318-46550337 MS.gene049759:CDS 40.0%
TGTGAACTCTGAACCACAAA+TGG - chr4.2:46550470-46550489 None:intergenic 40.0%
TTCCAAATGGGGAATCATCA+TGG + chr4.2:46550397-46550416 MS.gene049759:CDS 40.0%
!! ACCATTGATTTCTGTGTAGC+CGG - chr4.2:46550658-46550677 None:intergenic 40.0%
!! TTGATTCTTCTAGCTCAAGG+TGG + chr4.2:46550052-46550071 MS.gene049759:CDS 40.0%
AAGAAGCTGAGAAACCTCGT+GGG + chr4.2:46550362-46550381 MS.gene049759:CDS 45.0%
ACTCATCACTATTCGCAACC+AGG - chr4.2:46550339-46550358 None:intergenic 45.0%
CCAAAGGAAAACGTACCAAG+AGG + chr4.2:46549909-46549928 MS.gene049759:CDS 45.0%
CCCCATTTGGAAAGAAACCA+TGG - chr4.2:46550389-46550408 None:intergenic 45.0%
GATGCAACTCACATAGCCAA+AGG + chr4.2:46549893-46549912 MS.gene049759:CDS 45.0%
GCCCATGGTTTCTTTCCAAA+TGG + chr4.2:46550384-46550403 MS.gene049759:CDS 45.0%
GGTGAGACTAGCACAAAACT+TGG + chr4.2:46550136-46550155 MS.gene049759:CDS 45.0%
TCCGGCTACACAGAAATCAA+TGG + chr4.2:46550654-46550673 MS.gene049759:CDS 45.0%
TGTGGTTCAGAGTTCACATC+CGG + chr4.2:46550472-46550491 MS.gene049759:CDS 45.0%
TTCACATCCGGACAAGCATT+GGG + chr4.2:46550484-46550503 MS.gene049759:CDS 45.0%
! CCTCTTGGTACGTTTTCCTT+TGG - chr4.2:46549912-46549931 None:intergenic 45.0%
!! TCCTTGTGGTGTTGTTGCTA+TGG + chr4.2:46549946-46549965 MS.gene049759:CDS 45.0%
CCACCCAAAGCTTGAAAGGA+AGG - chr4.2:46550202-46550221 None:intergenic 50.0%
CTTGGCCTAACTCCTTCATC+CGG - chr4.2:46550565-46550584 None:intergenic 50.0%
GAAGAAGCTGAGAAACCTCG+TGG + chr4.2:46550361-46550380 MS.gene049759:CDS 50.0%
GCCATAGCAACAACACCACA+AGG - chr4.2:46549950-46549969 None:intergenic 50.0%
GTGACCACCCAAAGCTTGAA+AGG - chr4.2:46550206-46550225 None:intergenic 50.0%
GTTCACATCCGGACAAGCAT+TGG + chr4.2:46550483-46550502 MS.gene049759:CDS 50.0%
TAATGGCAACCCACCAAAGC+CGG - chr4.2:46550694-46550713 None:intergenic 50.0%
TTGGCCTAACTCCTTCATCC+GGG - chr4.2:46550564-46550583 None:intergenic 50.0%
! TGGACACATGAGGAGGCATA+GGG + chr4.2:46550507-46550526 MS.gene049759:CDS 50.0%
!! AAGCATTGGGTGGACACATG+AGG + chr4.2:46550497-46550516 MS.gene049759:CDS 50.0%
!! CCTTCCTTTCAAGCTTTGGG+TGG + chr4.2:46550199-46550218 MS.gene049759:CDS 50.0%
!! TCAAGCTTTGGGTGGTCACA+GGG + chr4.2:46550207-46550226 MS.gene049759:CDS 50.0%
!! TTCAAGCTTTGGGTGGTCAC+AGG + chr4.2:46550206-46550225 MS.gene049759:CDS 50.0%
AAAGAAACCATGGGCCCACG+AGG - chr4.2:46550379-46550398 None:intergenic 55.0%
CATTGGGTGGACACATGAGG+AGG + chr4.2:46550500-46550519 MS.gene049759:CDS 55.0%
CGGATGAAGGAGTTAGGCCA+AGG + chr4.2:46550563-46550582 MS.gene049759:CDS 55.0%
GGATCTTAACCTTCCGGCAC+CGG + chr4.2:46550600-46550619 MS.gene049759:CDS 55.0%
GTGTCCACCCAATGCTTGTC+CGG - chr4.2:46550494-46550513 None:intergenic 55.0%
GTTTCCCGGATATCCTCCTC+CGG - chr4.2:46550622-46550641 None:intergenic 55.0%
TGGCCTAACTCCTTCATCCG+GGG - chr4.2:46550563-46550582 None:intergenic 55.0%
TGTGATGAAGACTGTGGCCG+AGG - chr4.2:46550298-46550317 None:intergenic 55.0%
! GTGGACACATGAGGAGGCAT+AGG + chr4.2:46550506-46550525 MS.gene049759:CDS 55.0%
!! AATGGTGATGGCTGCTGCTC+CGG + chr4.2:46550672-46550691 MS.gene049759:CDS 55.0%
!! ACATCCGGACAAGCATTGGG+TGG + chr4.2:46550487-46550506 MS.gene049759:CDS 55.0%
TGAGAAACCTCGTGGGCCCA+TGG + chr4.2:46550369-46550388 MS.gene049759:CDS 60.0%
! GAGGAGGCATAGGGCATCAG+TGG + chr4.2:46550516-46550535 MS.gene049759:CDS 60.0%
! TCTTAACCTTCCGGCACCGG+AGG + chr4.2:46550603-46550622 MS.gene049759:CDS 60.0%
CGGATATCCTCCTCCGGTGC+CGG - chr4.2:46550616-46550635 None:intergenic 65.0%
CGGCCCCGGATGAAGGAGTT+AGG + chr4.2:46550557-46550576 MS.gene049759:CDS 65.0%
GATGGCTGCTGCTCCGGCTT+TGG + chr4.2:46550678-46550697 MS.gene049759:CDS 65.0%
GGCATCAGTGGCAAACGCCG+CGG + chr4.2:46550528-46550547 MS.gene049759:CDS 65.0%
TGCTGCTGCGGCGGTGATGA+TGG - chr4.2:46550274-46550293 None:intergenic 65.0%
! CGGCACCGGAGGAGGATATC+CGG + chr4.2:46550614-46550633 MS.gene049759:CDS 65.0%
! GCTGCTGCTCCGGCTTTGGT+GGG + chr4.2:46550682-46550701 MS.gene049759:CDS 65.0%
! GGCACCGGAGGAGGATATCC+GGG + chr4.2:46550615-46550634 MS.gene049759:CDS 65.0%
! TAACCTTCCGGCACCGGAGG+AGG + chr4.2:46550606-46550625 MS.gene049759:CDS 65.0%
! TATCCTCCTCCGGTGCCGGA+AGG - chr4.2:46550612-46550631 None:intergenic 65.0%
TGTGGCCGAGGCTGCTGCTG+CGG - chr4.2:46550286-46550305 None:intergenic 70.0%
! GGCTGCTGCTCCGGCTTTGG+TGG + chr4.2:46550681-46550700 MS.gene049759:CDS 70.0%
CACCGCCGCAGCAGCAGCCT+CGG + chr4.2:46550278-46550297 MS.gene049759:CDS 75.0%
GCTGTCGCGGCCCCGGATGA+AGG + chr4.2:46550550-46550569 MS.gene049759:CDS 75.0%
GGCAAACGCCGCGGCTGTCG+CGG + chr4.2:46550537-46550556 MS.gene049759:CDS 75.0%
! ATCCGGGGCCGCGACAGCCG+CGG - chr4.2:46550548-46550567 None:intergenic 80.0%
! GGCCGAGGCTGCTGCTGCGG+CGG - chr4.2:46550283-46550302 None:intergenic 80.0%
! CGCCGCGGCTGTCGCGGCCC+CGG + chr4.2:46550543-46550562 MS.gene049759:CDS 90.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 46549845 46550717 46549845 ID=MS.gene049759
chr4.2 mRNA 46549845 46550717 46549845 ID=MS.gene049759.t1;Parent=MS.gene049759
chr4.2 exon 46549845 46550717 46549845 ID=MS.gene049759.t1.exon1;Parent=MS.gene049759.t1
chr4.2 CDS 46549845 46550717 46549845 ID=cds.MS.gene049759.t1;Parent=MS.gene049759.t1
Gene Sequence

>MS.gene049759

ATGGAAGAGTTTGTTATGGAGGAAAAGATGGTGAGTTATGCTAAGAAAGATGCAACTCACATAGCCAAAGGAAAACGTACCAAGAGGTTAAGATTTTTGTCTCCTTGTGGTGTTGTTGCTATGGCATCTAGTTGTTCAAGTGATGAGAGCAACATTGATCTTGAAGAAGATGAAGAAGATGATGATGAAGAAGATTTGGCTAAGTGTTTGATTCTTCTAGCTCAAGGTGGAAATCATAGGCTTGTTGATGAGAATAAAAGGGAAAAGGGTAGTCATGGAAATAAAAAAATTGGTGAGACTAGCACAAAACTTGGTTTGTATATTTATGAATGCAAAACATGTAACCGAACTTTTCCTTCCTTTCAAGCTTTGGGTGGTCACAGGGCAAGTCACAAAAAGCCTAAAATTATGGCTGAGGAGAAGAAACCATCATCACCGCCGCAGCAGCAGCCTCGGCCACAGTCTTCATCACATGATTCGCAATCAGTTAACCTGGTTGCGAATAGTGATGAGTTTGAAGAAGCTGAGAAACCTCGTGGGCCCATGGTTTCTTTCCAAATGGGGAATCATCATGGTTTGAAAGCAGCTATTAATGGGAACAAGGCCAAGATTCATGAGTGTTCCATTTGTGGTTCAGAGTTCACATCCGGACAAGCATTGGGTGGACACATGAGGAGGCATAGGGCATCAGTGGCAAACGCCGCGGCTGTCGCGGCCCCGGATGAAGGAGTTAGGCCAAGGAATATTTTACAATTGGATCTTAACCTTCCGGCACCGGAGGAGGATATCCGGGAAACAAAGTTTCAGTTTCCGGCTACACAGAAATCAATGGTGATGGCTGCTGCTCCGGCTTTGGTGGGTTGCCATTATTAG
Protein sequence

>MS.gene049759.t1

MEEFVMEEKMVSYAKKDATHIAKGKRTKRLRFLSPCGVVAMASSCSSDESNIDLEEDEEDDDEEDLAKCLILLAQGGNHRLVDENKREKGSHGNKKIGETSTKLGLYIYECKTCNRTFPSFQALGGHRASHKKPKIMAEEKKPSSPPQQQPRPQSSSHDSQSVNLVANSDEFEEAEKPRGPMVSFQMGNHHGLKAAINGNKAKIHECSICGSEFTSGQALGGHMRRHRASVANAAAVAAPDEGVRPRNILQLDLNLPAPEEDIRETKFQFPATQKSMVMAAAPALVGCHY