Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04999.t1 | XP_003600679.1 | 93.7 | 239 | 14 | 1 | 1 | 239 | 1 | 238 | 2.80E-106 | 394.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04999.t1 | Q39263 | 48.9 | 182 | 62 | 7 | 86 | 239 | 82 | 260 | 7.9e-33 | 142.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04999.t1 | G7J8Q3 | 93.7 | 239 | 14 | 1 | 1 | 239 | 1 | 238 | 2.0e-106 | 394.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene04999.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04999.t1 | MTR_3g065020 | 96.234 | 239 | 8 | 1 | 1 | 239 | 1 | 238 | 3.54e-171 | 471 |
| MS.gene04999.t1 | MTR_5g077370 | 58.300 | 247 | 85 | 8 | 1 | 239 | 1 | 237 | 7.20e-85 | 252 |
| MS.gene04999.t1 | MTR_1g033340 | 43.028 | 251 | 125 | 7 | 3 | 239 | 5 | 251 | 5.36e-59 | 187 |
| MS.gene04999.t1 | MTR_2g104900 | 65.432 | 81 | 20 | 3 | 81 | 154 | 76 | 155 | 8.37e-27 | 103 |
| MS.gene04999.t1 | MTR_1g013130 | 52.000 | 100 | 30 | 3 | 74 | 155 | 80 | 179 | 1.99e-25 | 100 |
| MS.gene04999.t1 | MTR_1g107055 | 40.299 | 134 | 74 | 4 | 70 | 202 | 47 | 175 | 3.85e-24 | 95.9 |
| MS.gene04999.t1 | MTR_3g106230 | 49.541 | 109 | 30 | 6 | 72 | 159 | 60 | 164 | 3.77e-20 | 85.9 |
| MS.gene04999.t1 | MTR_4g006575 | 62.857 | 70 | 18 | 3 | 87 | 155 | 67 | 129 | 1.34e-18 | 81.6 |
| MS.gene04999.t1 | MTR_4g006600 | 62.857 | 70 | 18 | 3 | 87 | 155 | 67 | 129 | 1.34e-18 | 81.6 |
| MS.gene04999.t1 | MTR_4g128340 | 66.071 | 56 | 18 | 1 | 86 | 140 | 44 | 99 | 4.13e-17 | 76.6 |
| MS.gene04999.t1 | MTR_8g466760 | 48.780 | 82 | 32 | 4 | 87 | 160 | 98 | 177 | 7.46e-14 | 69.7 |
| MS.gene04999.t1 | MTR_3g011990 | 43.529 | 85 | 37 | 3 | 87 | 165 | 98 | 177 | 2.15e-12 | 65.5 |
| MS.gene04999.t1 | MTR_7g083210 | 75.758 | 33 | 8 | 0 | 87 | 119 | 39 | 71 | 2.66e-12 | 63.9 |
| MS.gene04999.t1 | MTR_7g086860 | 67.568 | 37 | 12 | 0 | 87 | 123 | 30 | 66 | 5.91e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene04999.t1 | AT1G66140 | 48.913 | 184 | 59 | 8 | 86 | 239 | 82 | 260 | 6.85e-45 | 151 |
| MS.gene04999.t1 | AT1G24625 | 40.291 | 206 | 85 | 11 | 46 | 239 | 30 | 209 | 5.41e-31 | 114 |
| MS.gene04999.t1 | AT1G80730 | 53.261 | 92 | 25 | 2 | 82 | 155 | 61 | 152 | 1.12e-23 | 95.5 |
| MS.gene04999.t1 | AT5G10970 | 45.902 | 122 | 50 | 5 | 47 | 155 | 56 | 174 | 1.14e-22 | 93.6 |
| MS.gene04999.t1 | AT5G25160 | 51.579 | 95 | 35 | 3 | 67 | 155 | 44 | 133 | 5.96e-22 | 90.9 |
| MS.gene04999.t1 | AT5G57520 | 52.113 | 71 | 34 | 0 | 87 | 157 | 50 | 120 | 2.80e-16 | 73.9 |
| MS.gene04999.t1 | AT5G14010 | 68.421 | 38 | 12 | 0 | 87 | 124 | 36 | 73 | 1.39e-13 | 67.0 |
| MS.gene04999.t1 | AT3G58070 | 55.357 | 56 | 21 | 1 | 67 | 122 | 73 | 124 | 9.20e-12 | 63.5 |
| MS.gene04999.t1 | AT5G01860 | 60.000 | 40 | 16 | 0 | 87 | 126 | 65 | 104 | 1.72e-11 | 62.0 |
Find 41 sgRNAs with CRISPR-Local
Find 55 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCCTCTCAATGGTTCTTTC+AGG | 0.205977 | 3.3:-58655375 | MS.gene04999:CDS |
| CAAAGTTTCAATGTTAATTC+AGG | 0.241054 | 3.3:+58655088 | None:intergenic |
| AATGGTTCTTTCAGGTCATT+AGG | 0.297972 | 3.3:-58655367 | MS.gene04999:CDS |
| AATTGCAAGAGAAAATTCTT+TGG | 0.318566 | 3.3:+58655530 | None:intergenic |
| TTTCTCTCTTGTGCGCGTTT+TGG | 0.325608 | 3.3:+58655464 | None:intergenic |
| AACCTGGAGAGGTTTCAATT+TGG | 0.372940 | 3.3:+58655695 | None:intergenic |
| GCAAAGCGAGCTATGCGAAT+TGG | 0.383652 | 3.3:-58655433 | MS.gene04999:CDS |
| TATCGGTCATCCGATATTCT+TGG | 0.398517 | 3.3:-58655248 | MS.gene04999:CDS |
| CTGGAGAGGTTTCAATTTGG+TGG | 0.433921 | 3.3:+58655698 | None:intergenic |
| TCATCGTCCTCCAAGAATAT+CGG | 0.434665 | 3.3:+58655238 | None:intergenic |
| AATTTGGTGGGTGGATATAG+TGG | 0.440124 | 3.3:+58655711 | None:intergenic |
| TAATTCAGATTCCATCTCAC+TGG | 0.457739 | 3.3:-58655653 | MS.gene04999:CDS |
| AAGTTCGTTGCTGCTCTCAC+TGG | 0.464039 | 3.3:+58655558 | None:intergenic |
| TTGGTTGCTCGAGATTCAAC+AGG | 0.465065 | 3.3:-58655598 | MS.gene04999:CDS |
| TCAGGGCTAGGCCTTATTGT+TGG | 0.467517 | 3.3:+58655304 | None:intergenic |
| GCAAGAGAAAATTCTTTGGA+TGG | 0.480537 | 3.3:+58655534 | None:intergenic |
| AGCAGTGCTAGATTCAAGTT+AGG | 0.490778 | 3.3:-58655274 | MS.gene04999:CDS |
| GGGCTAGGCCTTATTGTTGG+TGG | 0.506009 | 3.3:+58655307 | None:intergenic |
| CTTGCATCTCTGCCTCTCAA+TGG | 0.509849 | 3.3:-58655385 | MS.gene04999:CDS |
| TGGAGAGGTTTCAATTTGGT+GGG | 0.530109 | 3.3:+58655699 | None:intergenic |
| CACCAAATTGAAACCTCTCC+AGG | 0.534773 | 3.3:-58655697 | MS.gene04999:CDS |
| AGATGGAATCTGAATTAGAA+AGG | 0.546754 | 3.3:+58655659 | None:intergenic |
| ACACACTCTTCACTACATCA+TGG | 0.560641 | 3.3:-58655337 | MS.gene04999:CDS |
| AGATTCAAGTTAGGATCTAT+CGG | 0.563874 | 3.3:-58655265 | MS.gene04999:CDS |
| AGGCTGTTACTGTAGGAACC+TGG | 0.567514 | 3.3:+58655679 | None:intergenic |
| CGGTCATCCGATATTCTTGG+AGG | 0.568620 | 3.3:-58655245 | MS.gene04999:CDS |
| TTTCAACAAGAACAATGACT+TGG | 0.569063 | 3.3:-58655617 | MS.gene04999:CDS |
| GAATTCTCTATGTCAACCAA+TGG | 0.572984 | 3.3:+58655115 | None:intergenic |
| AGAGGTTTCAATTTGGTGGG+TGG | 0.575688 | 3.3:+58655702 | None:intergenic |
| GAGTGTTAAGTCCAGTGAGA+TGG | 0.580909 | 3.3:+58655642 | None:intergenic |
| ATCAGATTCATATTCTTCTG+TGG | 0.583159 | 3.3:+58655753 | None:intergenic |
| TTTCATCAGGTTACTAAGGG+AGG | 0.593477 | 3.3:-58655190 | MS.gene04999:CDS |
| ATTAGAAAGGCTGTTACTGT+AGG | 0.605208 | 3.3:+58655672 | None:intergenic |
| TGAGGTGAATGTGAAACCAT+TGG | 0.606667 | 3.3:-58655131 | MS.gene04999:CDS |
| TACAGCTCTCAGGCGCTCGG+TGG | 0.607796 | 3.3:-58655490 | MS.gene04999:CDS |
| TGGATATAGTGGAAGCAACA+TGG | 0.607959 | 3.3:+58655722 | None:intergenic |
| ACGATGAGACAGAACTGATG+TGG | 0.646574 | 3.3:-58655222 | MS.gene04999:CDS |
| GAGACAGAACTGATGTGGCA+AGG | 0.647919 | 3.3:-58655217 | MS.gene04999:CDS |
| GTTACTGTAGGAACCTGGAG+AGG | 0.681388 | 3.3:+58655684 | None:intergenic |
| GACCTGAAAGAACCATTGAG+AGG | 0.682870 | 3.3:+58655373 | None:intergenic |
| GAATTGGACTATTCACTGAG+AGG | 0.709982 | 3.3:-58655417 | MS.gene04999:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATTGCAAGAGAAAATTCTT+TGG | + | chr3.3:58655344-58655363 | None:intergenic | 25.0% |
| ! | CAAAGTTTCAATGTTAATTC+AGG | + | chr3.3:58655786-58655805 | None:intergenic | 25.0% |
| ! | TCTACTTCAAGATCAAAAAT+TGG | + | chr3.3:58655096-58655115 | None:intergenic | 25.0% |
| !! | AATTTTTGATCTTGAAGTAG+AGG | - | chr3.3:58655095-58655114 | MS.gene04999:CDS | 25.0% |
| !!! | TTCAAATATGTGTTTTAGTG+AGG | - | chr3.3:58655722-58655741 | MS.gene04999:CDS | 25.0% |
| AGATGGAATCTGAATTAGAA+AGG | + | chr3.3:58655215-58655234 | None:intergenic | 30.0% | |
| ATCAGATTCATATTCTTCTG+TGG | + | chr3.3:58655121-58655140 | None:intergenic | 30.0% | |
| TTTCAACAAGAACAATGACT+TGG | - | chr3.3:58655254-58655273 | MS.gene04999:CDS | 30.0% | |
| ! | AGATTCAAGTTAGGATCTAT+CGG | - | chr3.3:58655606-58655625 | MS.gene04999:CDS | 30.0% |
| ! | AGTTTTCATCAGGTTACTAA+GGG | - | chr3.3:58655678-58655697 | MS.gene04999:CDS | 30.0% |
| ! | TAGTTTTCATCAGGTTACTA+AGG | - | chr3.3:58655677-58655696 | MS.gene04999:CDS | 30.0% |
| ! | TTTTCTCTTGCAATTACTGT+CGG | - | chr3.3:58655349-58655368 | MS.gene04999:CDS | 30.0% |
| ATTAGAAAGGCTGTTACTGT+AGG | + | chr3.3:58655202-58655221 | None:intergenic | 35.0% | |
| GAATTCTCTATGTCAACCAA+TGG | + | chr3.3:58655759-58655778 | None:intergenic | 35.0% | |
| GCAAGAGAAAATTCTTTGGA+TGG | + | chr3.3:58655340-58655359 | None:intergenic | 35.0% | |
| TAATTCAGATTCCATCTCAC+TGG | - | chr3.3:58655218-58655237 | MS.gene04999:CDS | 35.0% | |
| ! | AATGGTTCTTTCAGGTCATT+AGG | - | chr3.3:58655504-58655523 | MS.gene04999:CDS | 35.0% |
| !!! | TAGCACTGCTTTTTATCTCA+GGG | + | chr3.3:58655587-58655606 | None:intergenic | 35.0% |
| AACCTGGAGAGGTTTCAATT+TGG | + | chr3.3:58655179-58655198 | None:intergenic | 40.0% | |
| ACACACTCTTCACTACATCA+TGG | - | chr3.3:58655534-58655553 | MS.gene04999:CDS | 40.0% | |
| GAATTGGACTATTCACTGAG+AGG | - | chr3.3:58655454-58655473 | MS.gene04999:CDS | 40.0% | |
| TATCGGTCATCCGATATTCT+TGG | - | chr3.3:58655623-58655642 | MS.gene04999:CDS | 40.0% | |
| TCATCGTCCTCCAAGAATAT+CGG | + | chr3.3:58655636-58655655 | None:intergenic | 40.0% | |
| TGAGGTGAATGTGAAACCAT+TGG | - | chr3.3:58655740-58655759 | MS.gene04999:CDS | 40.0% | |
| TGGAGAGGTTTCAATTTGGT+GGG | + | chr3.3:58655175-58655194 | None:intergenic | 40.0% | |
| TTTCATCAGGTTACTAAGGG+AGG | - | chr3.3:58655681-58655700 | MS.gene04999:CDS | 40.0% | |
| ! | AATTTGGTGGGTGGATATAG+TGG | + | chr3.3:58655163-58655182 | None:intergenic | 40.0% |
| ! | AGCAGTGCTAGATTCAAGTT+AGG | - | chr3.3:58655597-58655616 | MS.gene04999:CDS | 40.0% |
| ! | GTTTTTCGCCACCAACAATA+AGG | - | chr3.3:58655556-58655575 | MS.gene04999:CDS | 40.0% |
| ! | TGGATATAGTGGAAGCAACA+TGG | + | chr3.3:58655152-58655171 | None:intergenic | 40.0% |
| !!! | CTAGCACTGCTTTTTATCTC+AGG | + | chr3.3:58655588-58655607 | None:intergenic | 40.0% |
| ACGATGAGACAGAACTGATG+TGG | - | chr3.3:58655649-58655668 | MS.gene04999:CDS | 45.0% | |
| CACCAAATTGAAACCTCTCC+AGG | - | chr3.3:58655174-58655193 | MS.gene04999:CDS | 45.0% | |
| CTGGAGAGGTTTCAATTTGG+TGG | + | chr3.3:58655176-58655195 | None:intergenic | 45.0% | |
| GACCTGAAAGAACCATTGAG+AGG | + | chr3.3:58655501-58655520 | None:intergenic | 45.0% | |
| GAGTGTTAAGTCCAGTGAGA+TGG | + | chr3.3:58655232-58655251 | None:intergenic | 45.0% | |
| TGCCTCTCAATGGTTCTTTC+AGG | - | chr3.3:58655496-58655515 | MS.gene04999:CDS | 45.0% | |
| TTGGTTGCTCGAGATTCAAC+AGG | - | chr3.3:58655273-58655292 | MS.gene04999:CDS | 45.0% | |
| ! | AGAGGTTTCAATTTGGTGGG+TGG | + | chr3.3:58655172-58655191 | None:intergenic | 45.0% |
| ! | GCGCAAATTTTACAGCTCTC+AGG | - | chr3.3:58655371-58655390 | MS.gene04999:CDS | 45.0% |
| ! | GTGGCAAGGTAGTTTTCATC+AGG | - | chr3.3:58655668-58655687 | MS.gene04999:CDS | 45.0% |
| ! | TTTCTCTCTTGTGCGCGTTT+TGG | + | chr3.3:58655410-58655429 | None:intergenic | 45.0% |
| !!! | CTGCTTTTTATCTCAGGGCT+AGG | + | chr3.3:58655582-58655601 | None:intergenic | 45.0% |
| AAGTTCGTTGCTGCTCTCAC+TGG | + | chr3.3:58655316-58655335 | None:intergenic | 50.0% | |
| AGGCTGTTACTGTAGGAACC+TGG | + | chr3.3:58655195-58655214 | None:intergenic | 50.0% | |
| CGGTCATCCGATATTCTTGG+AGG | - | chr3.3:58655626-58655645 | MS.gene04999:CDS | 50.0% | |
| CTTGCATCTCTGCCTCTCAA+TGG | - | chr3.3:58655486-58655505 | MS.gene04999:CDS | 50.0% | |
| GAGACAGAACTGATGTGGCA+AGG | - | chr3.3:58655654-58655673 | MS.gene04999:CDS | 50.0% | |
| GCAAAGCGAGCTATGCGAAT+TGG | - | chr3.3:58655438-58655457 | MS.gene04999:CDS | 50.0% | |
| GTTACTGTAGGAACCTGGAG+AGG | + | chr3.3:58655190-58655209 | None:intergenic | 50.0% | |
| ! | TTTTACAGCTCTCAGGCGCT+CGG | - | chr3.3:58655378-58655397 | MS.gene04999:CDS | 50.0% |
| !! | TCAGGGCTAGGCCTTATTGT+TGG | + | chr3.3:58655570-58655589 | None:intergenic | 50.0% |
| ! | CTCTCTTGTGCGCGTTTTGG+TGG | + | chr3.3:58655407-58655426 | None:intergenic | 55.0% |
| !! | GGGCTAGGCCTTATTGTTGG+TGG | + | chr3.3:58655567-58655586 | None:intergenic | 55.0% |
| TACAGCTCTCAGGCGCTCGG+TGG | - | chr3.3:58655381-58655400 | MS.gene04999:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58655087 | 58655806 | 58655087 | ID=MS.gene04999 |
| chr3.3 | mRNA | 58655087 | 58655806 | 58655087 | ID=MS.gene04999.t1;Parent=MS.gene04999 |
| chr3.3 | exon | 58655087 | 58655806 | 58655087 | ID=MS.gene04999.t1.exon1;Parent=MS.gene04999.t1 |
| chr3.3 | CDS | 58655087 | 58655806 | 58655087 | ID=cds.MS.gene04999.t1;Parent=MS.gene04999.t1 |
| Gene Sequence |
| Protein sequence |
