AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050057

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050057.t1 MTR_5g065200 90.866 843 61 5 1 833 1 837 0.0 1510
MS.gene050057.t1 MTR_1g114150 70.545 808 197 9 46 832 265 1052 0.0 1152
MS.gene050057.t1 MTR_1g114130 68.362 806 217 8 45 831 253 1039 0.0 1107
MS.gene050057.t1 MTR_1g069565 62.905 895 255 10 3 832 1 883 0.0 1072
MS.gene050057.t1 MTR_1g114070 70.225 712 205 2 127 832 216 926 0.0 1032
MS.gene050057.t1 MTR_1g069565 57.563 952 270 13 3 832 1 940 0.0 1004
MS.gene050057.t1 MTR_1g114150 67.338 695 186 9 46 719 265 939 0.0 937
MS.gene050057.t1 MTR_5g047620 64.902 661 189 8 207 831 145 798 0.0 857
MS.gene050057.t1 MTR_0020s0100 61.192 621 211 11 207 813 87 691 0.0 745
MS.gene050057.t1 MTR_2g024270 45.333 825 387 17 24 828 37 817 0.0 624
MS.gene050057.t1 MTR_4g132540 44.906 746 323 13 129 828 161 864 0.0 612
MS.gene050057.t1 MTR_2g024140 47.159 704 323 12 129 828 27 685 0.0 603
MS.gene050057.t1 MTR_7g117445 40.497 805 357 18 132 829 136 925 0.0 548
MS.gene050057.t1 MTR_7g117445 40.497 805 357 18 132 829 136 925 0.0 548
MS.gene050057.t1 MTR_7g117445 40.497 805 357 18 132 829 136 925 0.0 548
MS.gene050057.t1 MTR_7g117445 38.523 745 336 18 132 769 136 865 1.41e-152 469
MS.gene050057.t1 MTR_1g083120 35.897 741 382 13 136 829 198 892 1.51e-148 460
MS.gene050057.t1 MTR_1g083120 35.897 741 382 13 136 829 215 909 2.68e-148 460
MS.gene050057.t1 MTR_1g083120 35.139 683 350 13 136 771 198 834 6.40e-130 409
MS.gene050057.t1 MTR_1g083120 35.139 683 350 13 136 771 198 834 6.94e-130 409
MS.gene050057.t1 MTR_1g008060 44.527 539 256 12 130 661 864 1366 1.98e-128 422
MS.gene050057.t1 MTR_1g008060 74.265 136 35 0 684 819 1551 1686 3.49e-58 217
MS.gene050057.t1 MTR_1g008060 44.527 539 256 12 130 661 855 1357 2.39e-128 422
MS.gene050057.t1 MTR_1g008060 74.265 136 35 0 684 819 1542 1677 3.70e-58 217
MS.gene050057.t1 MTR_2g024240 56.678 307 119 3 491 793 92 388 2.82e-110 343
MS.gene050057.t1 MTR_8g062940 47.956 367 159 4 136 502 33 367 1.36e-104 327
MS.gene050057.t1 MTR_2g011630 33.524 525 325 10 136 651 207 716 2.46e-81 281
MS.gene050057.t1 MTR_2g011630 48.993 149 76 0 684 832 810 958 5.08e-40 160
MS.gene050057.t1 MTR_2g011630 33.710 442 270 9 219 651 45 472 2.54e-67 238
MS.gene050057.t1 MTR_2g011630 48.993 149 76 0 684 832 566 714 2.27e-40 159
MS.gene050057.t1 MTR_2g011630 33.710 442 270 9 219 651 77 504 4.20e-67 238
MS.gene050057.t1 MTR_2g011630 48.993 149 76 0 684 832 598 746 2.62e-40 159
MS.gene050057.t1 MTR_0295s0020 62.424 165 62 0 504 668 12 176 1.66e-61 206
MS.gene050057.t1 MTR_4g091520 38.047 297 173 5 359 651 657 946 1.98e-57 214
MS.gene050057.t1 MTR_4g091520 35.533 197 119 4 108 297 230 425 4.04e-29 125
MS.gene050057.t1 MTR_4g091520 50.820 61 29 1 683 743 1062 1121 5.14e-11 67.0
MS.gene050057.t1 MTR_4g091520 38.047 297 173 5 359 651 727 1016 2.61e-57 214
MS.gene050057.t1 MTR_4g091520 35.533 197 119 4 108 297 230 425 4.86e-29 125
MS.gene050057.t1 MTR_4g091520 50.820 61 29 1 683 743 1132 1191 4.38e-11 67.4
MS.gene050057.t1 MTR_4g091520 38.047 297 173 5 359 651 692 981 2.61e-57 214
MS.gene050057.t1 MTR_4g091520 35.533 197 119 4 108 297 230 425 4.36e-29 125
MS.gene050057.t1 MTR_4g091520 50.820 61 29 1 683 743 1097 1156 4.81e-11 67.4
MS.gene050057.t1 MTR_4g091520 38.047 297 173 5 359 651 727 1016 2.91e-57 214
MS.gene050057.t1 MTR_4g091520 54.054 148 64 2 683 827 1132 1278 5.28e-43 169
MS.gene050057.t1 MTR_4g091520 35.533 197 119 4 108 297 230 425 6.52e-29 125
MS.gene050057.t1 MTR_8g465700 52.778 180 81 1 446 625 2 177 2.36e-55 192
MS.gene050057.t1 MTR_8g465720 75.281 89 21 1 683 771 52 139 7.86e-41 147
MS.gene050057.t1 MTR_8g013920 66.165 133 26 3 538 670 40 153 1.89e-39 145
MS.gene050057.t1 MTR_3g080750 71.739 46 13 0 625 670 86 131 2.17e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050057.t1 AT3G07610 46.445 844 385 11 41 832 67 895 0.0 706
MS.gene050057.t1 AT3G07610 45.423 863 388 12 41 832 67 917 0.0 689
MS.gene050057.t1 AT1G11950 49.429 700 331 7 132 828 189 868 0.0 678
MS.gene050057.t1 AT1G11950 49.429 700 331 7 132 828 189 868 0.0 678
MS.gene050057.t1 AT1G62310 48.986 690 321 11 135 812 208 878 0.0 651
MS.gene050057.t1 AT1G11950 47.077 633 312 7 132 761 189 801 0.0 573
MS.gene050057.t1 AT3G07610 42.476 638 306 10 41 631 67 690 1.12e-149 458
MS.gene050057.t1 AT4G00990 42.364 550 281 13 136 674 80 604 1.32e-131 412
MS.gene050057.t1 AT4G00990 60.177 113 40 2 665 777 671 778 2.24e-34 141
MS.gene050057.t1 AT4G00990 42.364 550 281 13 136 674 80 604 1.41e-131 411
MS.gene050057.t1 AT4G00990 63.810 105 33 2 665 769 671 770 1.41e-33 139
MS.gene050057.t1 AT4G00990 42.364 550 281 13 136 674 80 604 5.29e-131 412
MS.gene050057.t1 AT4G00990 61.350 163 58 2 665 827 671 828 5.60e-56 207
MS.gene050057.t1 AT1G09060 32.038 746 411 15 129 829 197 891 2.37e-119 384
MS.gene050057.t1 AT1G09060 32.038 746 411 15 129 829 197 891 2.37e-119 384
MS.gene050057.t1 AT1G09060 32.038 746 411 15 129 829 211 905 5.22e-119 383
MS.gene050057.t1 AT1G09060 32.038 746 411 15 129 829 211 905 5.22e-119 383
MS.gene050057.t1 AT4G21430 30.013 743 431 12 131 832 222 916 1.43e-100 333

Find 188 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCACCATGCAGATAATT+TGG 0.144590 5.4:-50679081 None:intergenic
TTTGTATCTGGTTACTTTCT+TGG 0.160659 5.4:-50679106 None:intergenic
GGATTTGTATATTCTTTAAA+TGG 0.213495 5.4:-50680088 None:intergenic
AGTACATGCGCTCATCATTA+TGG 0.215750 5.4:-50678827 None:intergenic
GATCTTCATCACGATGATTT+AGG 0.227135 5.4:+50679471 MS.gene050057:CDS
ACTAACTTTACCTCCTTTAC+AGG 0.233581 5.4:-50681118 None:intergenic
CTTCGTTGTGGGCAGCTTCT+AGG 0.236807 5.4:+50679018 MS.gene050057:CDS
TCTTTACTGGGTACACAAAT+TGG 0.244568 5.4:+50679954 MS.gene050057:CDS
ATTCCTTAGATGGTGCTCTT+TGG 0.245043 5.4:+50681011 MS.gene050057:CDS
AATGTGCACCAGTTCTTTAC+TGG 0.257653 5.4:+50679941 MS.gene050057:CDS
TTCCTTAGATGGTGCTCTTT+GGG 0.262549 5.4:+50681012 MS.gene050057:CDS
AGTACATTGCTGACAATTAC+AGG 0.278275 5.4:-50679525 None:intergenic
AAACAAGAACAAGTCACTTT+TGG 0.285165 5.4:+50679614 MS.gene050057:CDS
AGCATCAGGTGCTTTGTATC+TGG 0.288535 5.4:-50679118 None:intergenic
GTTGGTGGGGATTCCTTAGA+TGG 0.294738 5.4:+50681001 MS.gene050057:CDS
TCACAATTAATCTCAGGTTT+TGG 0.298428 5.4:-50679213 None:intergenic
CTTTCTTCAAATAGTTTAGA+AGG 0.302686 5.4:-50680028 None:intergenic
TACCTCCAATTTGTCCTCTA+TGG 0.305460 5.4:-50683902 None:intergenic
TCATATTGCTGAAGCAAAAC+TGG 0.320106 5.4:+50680862 MS.gene050057:CDS
TCTATGGACCTGTGATTTAC+TGG 0.329135 5.4:-50683886 None:intergenic
AGCATTCTGTGCAGCTTCTA+TGG 0.339021 5.4:-50678963 None:intergenic
GGAGCTACATGGTGATATTC+AGG 0.343116 5.4:+50680946 MS.gene050057:CDS
ATGCCCGATGGTTAGATAAA+AGG 0.344516 5.4:-50682416 None:intergenic
ATTCCTACATGGTCAAAATC+TGG 0.346904 5.4:+50679156 MS.gene050057:CDS
AGGCCAATTCTGTTTATCCT+TGG 0.348155 5.4:-50679978 None:intergenic
ATCCCAAAGAGCACCATCTA+AGG 0.353655 5.4:-50681014 None:intergenic
GCTCTTGTTTGAATCCTGTT+AGG 0.355578 5.4:+50679361 MS.gene050057:CDS
GGCCAATTCTGTTTATCCTT+GGG 0.360073 5.4:-50679977 None:intergenic
AGAAGCATTTCAGAGAGTTC+AGG 0.389778 5.4:+50681080 MS.gene050057:CDS
GTACATGCGCTCATCATTAT+GGG 0.392626 5.4:-50678826 None:intergenic
ATATCATCTCTATTCCTAAC+AGG 0.396727 5.4:-50679375 None:intergenic
AAGTATTGAAATTGAAAGAT+TGG 0.403818 5.4:+50680002 MS.gene050057:CDS
ACTAACTCAGAGATGCATTC+TGG 0.406256 5.4:-50679270 None:intergenic
AGAGGACGTACCTGCATTGC+AGG 0.406514 5.4:+50681048 MS.gene050057:CDS
CAGCTCTTTGCAAAGAATCC+AGG 0.410316 5.4:+50679418 MS.gene050057:CDS
TCTTCCACATTTAATACGAT+TGG 0.410475 5.4:+50678445 MS.gene050057:CDS
CTTCACCATGCAGATAATTT+GGG 0.410728 5.4:-50679080 None:intergenic
TCTTTCTAGAATAGACATAA+TGG 0.411099 5.4:-50677434 None:intergenic
TTCAATGGCACTGGAGCAAA+GGG 0.413215 5.4:+50679499 MS.gene050057:CDS
TTTGACCATGTAGGAATCTC+AGG 0.414130 5.4:-50679150 None:intergenic
TCAGGCTGGGCACCAGCATC+AGG 0.415198 5.4:-50679132 None:intergenic
GATAAGAAGAACAACAATAA+AGG 0.415697 5.4:+50677596 MS.gene050057:CDS
AATGTACTTGAATGTACATC+TGG 0.421952 5.4:+50679540 MS.gene050057:CDS
TATGGATTTGCTCAGGAGCT+TGG 0.422137 5.4:+50680193 MS.gene050057:CDS
TTACCTTCAGATTTCGGACT+TGG 0.425214 5.4:-50682632 None:intergenic
AAACCACCGGTAAACGTAAC+CGG 0.429322 5.4:+50677523 MS.gene050057:CDS
AAAATAGTTTGTTGGTTGGT+GGG 0.432080 5.4:+50680987 MS.gene050057:CDS
AAAACAGACTCCTCACAATA+AGG 0.433520 5.4:+50677725 MS.gene050057:CDS
AACAAACGCGGGAGCGGTGG+CGG 0.439355 5.4:+50677464 MS.gene050057:CDS
AGGCACTTGATTGCTTAGAT+TGG 0.440544 5.4:+50679643 MS.gene050057:CDS
TTCAGATATTCCTGCAATGC+AGG 0.441628 5.4:-50681058 None:intergenic
AACAATAAAGGCTCCCGTCT+TGG 0.441667 5.4:+50677608 MS.gene050057:CDS
GGCCCAAGGATAAACAGAAT+TGG 0.443826 5.4:+50679975 MS.gene050057:CDS
AAGATAAACTCAAAATCAAA+TGG 0.449448 5.4:-50679051 None:intergenic
AATGTACATCTGGTTTAAGC+TGG 0.451500 5.4:+50679550 MS.gene050057:CDS
AAGAGCTTTGAGTATATACT+TGG 0.456205 5.4:-50678423 None:intergenic
GACAAAATCAAGAGCAACCT+TGG 0.458966 5.4:-50683809 None:intergenic
TCTGGCTTTAGAACTTCATC+AGG 0.461474 5.4:-50680145 None:intergenic
GTCAAAGAAATGACAGTGGC+AGG 0.462164 5.4:+50678018 MS.gene050057:CDS
TTCTTCTTGTCTACCAAGAC+GGG 0.465663 5.4:-50677621 None:intergenic
CAGGTCCATAGAGGACAAAT+TGG 0.466017 5.4:+50683897 MS.gene050057:CDS
TTCAGATTTCGGACTTGGTG+GGG 0.467992 5.4:-50682627 None:intergenic
ATTTGCTCAGGAGCTTGGAC+GGG 0.473572 5.4:+50680198 MS.gene050057:CDS
GCAAAGCTTGTTTGAAGTCA+CGG 0.474647 5.4:+50678241 MS.gene050057:CDS
CCTTCAGATTTCGGACTTGG+TGG 0.476898 5.4:-50682629 None:intergenic
AAACGCGGGAGCGGTGGCGG+AGG 0.477252 5.4:+50677467 MS.gene050057:CDS
ACGATTGGATCAAGATCAAA+TGG 0.478348 5.4:+50678460 MS.gene050057:CDS
GATTTGCTCAGGAGCTTGGA+CGG 0.479287 5.4:+50680197 MS.gene050057:CDS
CATGCAGATAATTTGGGCCT+CGG 0.479508 5.4:-50679074 None:intergenic
ACTACAGTCAATTAAAGAAA+AGG 0.482579 5.4:+50678364 MS.gene050057:intron
TATTGCTTATGGATTTGCTC+AGG 0.486399 5.4:+50680186 MS.gene050057:CDS
GTTGCCGTGAGCTTCGTTGT+GGG 0.489346 5.4:+50679007 MS.gene050057:CDS
TTGTGTACCCAGTAAAGAAC+TGG 0.495127 5.4:-50679949 None:intergenic
CTTCAGATTTCGGACTTGGT+GGG 0.495361 5.4:-50682628 None:intergenic
CCGTGATCACAATTAATCTC+AGG 0.496158 5.4:-50679219 None:intergenic
CACTGTTGAAAATAGTTTGT+TGG 0.496892 5.4:+50680979 MS.gene050057:CDS
TTTCTTCTTGTCTACCAAGA+CGG 0.504344 5.4:-50677622 None:intergenic
GTTGTGGGCAGCTTCTAGGT+GGG 0.507656 5.4:+50679022 MS.gene050057:CDS
GGAAACCAACGATCATCATC+AGG 0.509624 5.4:+50678391 MS.gene050057:CDS
TGTATGTAGGTATTGAGCCT+TGG 0.510562 5.4:+50682556 MS.gene050057:intron
TGAGTTAGTATGTAAAGTAA+AGG 0.511034 5.4:+50679284 MS.gene050057:CDS
TGTATATTCTTTAAATGGCA+AGG 0.512473 5.4:-50680083 None:intergenic
CGTTGTGGGCAGCTTCTAGG+TGG 0.513332 5.4:+50679021 MS.gene050057:CDS
TGTCCATTGCTCTCCTGTAA+AGG 0.514874 5.4:+50681105 MS.gene050057:CDS
TTTCAATGGCACTGGAGCAA+AGG 0.515097 5.4:+50679498 MS.gene050057:CDS
GGCGGAATTCCTCTGTCAAA+CGG 0.517791 5.4:-50683858 None:intergenic
TCAAGATCAAATGGCTGAGA+AGG 0.518447 5.4:+50678469 MS.gene050057:CDS
GCTCCCTCTTGTCTTGCTCA+AGG 0.519343 5.4:-50680928 None:intergenic
GAAAATAGTTTGTTGGTTGG+TGG 0.519502 5.4:+50680986 MS.gene050057:CDS
AACAACAACACATTCACGAC+GGG 0.521041 5.4:+50677393 MS.gene050057:CDS
TGTTGCCGTGAGCTTCGTTG+TGG 0.522924 5.4:+50679006 MS.gene050057:CDS
GGAAGAAGAAGCAGCTTCAT+CGG 0.527580 5.4:-50677550 None:intergenic
TCTTTCAATTTCAATACTTG+AGG 0.529125 5.4:-50679998 None:intergenic
TTCCTACATGGTCAAAATCT+GGG 0.531797 5.4:+50679157 MS.gene050057:CDS
AAATAGTTTGTTGGTTGGTG+GGG 0.532716 5.4:+50680988 MS.gene050057:CDS
ACCATGTAGGAATCTCAGGC+TGG 0.533279 5.4:-50679146 None:intergenic
CCTGAGATTAATTGTGATCA+CGG 0.533747 5.4:+50679219 MS.gene050057:CDS
TTTGTCTCTCCTGAAAATGT+TGG 0.534278 5.4:+50683826 MS.gene050057:CDS
TCAGATTTCGGACTTGGTGG+GGG 0.534353 5.4:-50682626 None:intergenic
AATGCACGCCACATGACAAA+CGG 0.544435 5.4:-50679576 None:intergenic
GTCAATTAAAGAAAAGGAGG+AGG 0.545970 5.4:+50678370 MS.gene050057:intron
GAGATAGAAGCCAAGATACA+AGG 0.546758 5.4:+50678491 MS.gene050057:CDS
TTTGCTCAGGAGCTTGGACG+GGG 0.547453 5.4:+50680199 MS.gene050057:CDS
ATAATGATGAGCGCATGTAC+TGG 0.548032 5.4:+50678829 MS.gene050057:CDS
AGCTCTTTGCAAAGAATCCA+GGG 0.549815 5.4:+50679419 MS.gene050057:CDS
AGGTTAAGAGCACCTTTGAA+AGG 0.550974 5.4:-50680109 None:intergenic
TTAGATAAAAGGTCTGATCA+TGG 0.552811 5.4:-50682405 None:intergenic
ACCTGTGATTTACTGGAAGT+TGG 0.553493 5.4:-50683879 None:intergenic
TCAGATGCACCAAATGCAAA+CGG 0.554343 5.4:+50678045 MS.gene050057:CDS
TAAAAGGTCTGATCATGGAT+GGG 0.555220 5.4:-50682400 None:intergenic
ATAAAAGGTCTGATCATGGA+TGG 0.557609 5.4:-50682401 None:intergenic
ATGTGCACCAGTTCTTTACT+GGG 0.558976 5.4:+50679942 MS.gene050057:CDS
CTCAACCTGATGATGATCGT+TGG 0.558986 5.4:-50678396 None:intergenic
ACTCAGAACAAACGCGGGAG+CGG 0.564215 5.4:+50677458 MS.gene050057:CDS
AAGGTATCCTCGATTGAAAG+AGG 0.564928 5.4:+50678173 MS.gene050057:intron
GTTGAAAATAGTTTGTTGGT+TGG 0.566984 5.4:+50680983 MS.gene050057:CDS
GCTGGGAGCCGTTTGTCATG+TGG 0.567136 5.4:+50679568 MS.gene050057:CDS
GAATATACAAATCCTTTCAA+AGG 0.567529 5.4:+50680097 MS.gene050057:CDS
TGTGATTTACTGGAAGTTGG+CGG 0.568181 5.4:-50683876 None:intergenic
GCACCTTGAGCAAGACAAGA+GGG 0.570458 5.4:+50680925 MS.gene050057:CDS
TGATCCAATCGTATTAAATG+TGG 0.570512 5.4:-50678449 None:intergenic
AAGTTTACCGCGTCATTGTG+CGG 0.570831 5.4:+50680048 MS.gene050057:CDS
CTGAAGCAAAACTGGATCGT+GGG 0.571980 5.4:+50680870 MS.gene050057:CDS
ATGTACATCTGGTTTAAGCT+GGG 0.572127 5.4:+50679551 MS.gene050057:CDS
CTAGAATAGACATAATGGAT+AGG 0.573963 5.4:-50677429 None:intergenic
AGATACAAAGCACCTGATGC+TGG 0.574516 5.4:+50679120 MS.gene050057:CDS
AGCTTCTGAACAAAAGTCCA+AGG 0.575249 5.4:-50682573 None:intergenic
TTTCTTTCGAGAAATTGTGT+TGG 0.575306 5.4:-50677691 None:intergenic
CATCAGGCAGCTTCACAGCA+AGG 0.578623 5.4:-50680129 None:intergenic
TGATGAAGATCTACAGCCCT+AGG 0.579045 5.4:-50679459 None:intergenic
GCCAACTTCCAGTAAATCAC+AGG 0.581223 5.4:+50683878 MS.gene050057:CDS
CGAGGCCCAAATTATCTGCA+TGG 0.583825 5.4:+50679075 MS.gene050057:CDS
AGTTGTTCCTCTTTCAATCG+AGG 0.584177 5.4:-50678180 None:intergenic
AAGAAGAAGAATCTTGGTGA+TGG 0.584721 5.4:+50677651 MS.gene050057:CDS
GTCCATAGAGGACAAATTGG+AGG 0.587047 5.4:+50683900 MS.gene050057:CDS
CCTCCTTTACAGGAGAGCAA+TGG 0.587247 5.4:-50681108 None:intergenic
AGTAAATCACAGGTCCATAG+AGG 0.591019 5.4:+50683888 MS.gene050057:CDS
CAGAACAAACGCGGGAGCGG+TGG 0.591548 5.4:+50677461 MS.gene050057:CDS
CAAGACAAGAGGGAGCTACA+TGG 0.591607 5.4:+50680935 MS.gene050057:CDS
GCTGCCCACAACGAAGCTCA+CGG 0.592775 5.4:-50679011 None:intergenic
GAATAGACATAATGGATAGG+AGG 0.597179 5.4:-50677426 None:intergenic
TTGCAGTTACCACAGCAGAC+AGG 0.598583 5.4:-50678216 None:intergenic
TTGTGGGCAGCTTCTAGGTG+GGG 0.602777 5.4:+50679023 MS.gene050057:CDS
AAAAGGTCTGATCATGGATG+GGG 0.603357 5.4:-50682399 None:intergenic
TCTTCAAATAGTTTAGAAGG+AGG 0.604177 5.4:-50680025 None:intergenic
AGAATTTAAGCATACTGCAT+CGG 0.606233 5.4:-50680254 None:intergenic
ACAGTCAATTAAAGAAAAGG+AGG 0.606305 5.4:+50678367 MS.gene050057:intron
AAGAATCTTGGTGATGGTGA+TGG 0.607702 5.4:+50677657 MS.gene050057:CDS
CAATAAACAGTGGATTGAAG+AGG 0.607751 5.4:+50677974 MS.gene050057:intron
CCCAGCCTGAGATTCCTACA+TGG 0.609328 5.4:+50679145 MS.gene050057:CDS
AACGAAGCTCACGGCAACAG+AGG 0.614200 5.4:-50679002 None:intergenic
CCATGTAGGAATCTCAGGCT+GGG 0.616006 5.4:-50679145 None:intergenic
TTGACTCTTTCGAACAGCAA+CGG 0.617245 5.4:-50677571 None:intergenic
GAGGCATGTCCTGTCTGCTG+TGG 0.617423 5.4:+50678207 MS.gene050057:CDS
AGCACCTTGAGCAAGACAAG+AGG 0.618569 5.4:+50680924 MS.gene050057:CDS
TGAAGTTCTAAAGCCAGACA+TGG 0.620406 5.4:+50680150 MS.gene050057:CDS
AATGAAACTTCAAGATGCAG+AGG 0.623550 5.4:+50679320 MS.gene050057:CDS
GCTGAAGCAAAACTGGATCG+TGG 0.631664 5.4:+50680869 MS.gene050057:CDS
CTACATGGTGATATTCAGGA+TGG 0.633510 5.4:+50680950 MS.gene050057:CDS
CCATTGCTCTCCTGTAAAGG+AGG 0.635769 5.4:+50681108 MS.gene050057:CDS
CCACCAAGTCCGAAATCTGA+AGG 0.638088 5.4:+50682629 MS.gene050057:CDS
TCAAAGAAATGACAGTGGCA+GGG 0.638575 5.4:+50678019 MS.gene050057:CDS
TTGCTGTTCGAAAGAGTCAA+CGG 0.641060 5.4:+50677574 MS.gene050057:CDS
GAAAGAGGAACAACTTGCTG+AGG 0.642111 5.4:+50678188 MS.gene050057:CDS
CACGGCAACAGAGGAGACAA+AGG 0.649807 5.4:-50678993 None:intergenic
TGAGTGCTTCCGTTTGACAG+AGG 0.665002 5.4:+50683849 MS.gene050057:CDS
TTTCCGGTTACGTTTACCGG+TGG 0.665565 5.4:-50677526 None:intergenic
GAAGTTCTAAAGCCAGACAT+GGG 0.665837 5.4:+50680151 MS.gene050057:CDS
CAACAACAACACATTCACGA+CGG 0.666770 5.4:+50677392 MS.gene050057:CDS
TACATGCGCTCATCATTATG+GGG 0.667563 5.4:-50678825 None:intergenic
ATGATATACACAATAACACA+AGG 0.668038 5.4:+50679391 MS.gene050057:CDS
ACAATTTCTCGAAAGAAACC+CGG 0.668784 5.4:+50677696 MS.gene050057:CDS
TGGGTACACAAATTGGCCCA+AGG 0.673718 5.4:+50679961 MS.gene050057:CDS
ATAAACTCCGCACAATGACG+CGG 0.675233 5.4:-50680055 None:intergenic
GACGGGGTGATTCAGTGACT+AGG 0.676943 5.4:+50680215 MS.gene050057:CDS
GGGTGGCATGCTGATAGTGA+CGG 0.679311 5.4:+50679177 MS.gene050057:CDS
CGTATATGCTATGATTGATG+TGG 0.680480 5.4:+50684339 MS.gene050057:CDS
AAGAGACTCAGAACAAACGC+GGG 0.691625 5.4:+50677453 MS.gene050057:CDS
TGATTGCTTAGATTGGTGCG+AGG 0.693830 5.4:+50679650 MS.gene050057:CDS
GATGTTGTGACCTTATTGTG+AGG 0.707489 5.4:-50677735 None:intergenic
AAAGAGACTCAGAACAAACG+CGG 0.708789 5.4:+50677452 MS.gene050057:CDS
CTACATGGTCAAAATCTGGG+TGG 0.717604 5.4:+50679160 MS.gene050057:CDS
CTGCATCGGACATATCACAA+TGG 0.718824 5.4:-50680240 None:intergenic
CAATGTCAAAGAAATGACAG+TGG 0.718966 5.4:+50678014 MS.gene050057:CDS
GCTCTTTGCAAAGAATCCAG+GGG 0.726479 5.4:+50679420 MS.gene050057:CDS
TGATATACACAATAACACAA+GGG 0.738813 5.4:+50679392 MS.gene050057:CDS
ACAACAACACATTCACGACG+GGG 0.745848 5.4:+50677394 MS.gene050057:CDS
TCAATGGCACTGGAGCAAAG+GGG 0.769506 5.4:+50679500 MS.gene050057:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr5.4 gene 50677366 50684393 50677366 ID=MS.gene050057
chr5.4 mRNA 50677366 50684393 50677366 ID=MS.gene050057.t1;Parent=MS.gene050057
chr5.4 exon 50677366 50677746 50677366 ID=MS.gene050057.t1.exon1;Parent=MS.gene050057.t1
chr5.4 CDS 50677366 50677746 50677366 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50677984 50678099 50677984 ID=MS.gene050057.t1.exon2;Parent=MS.gene050057.t1
chr5.4 CDS 50677984 50678099 50677984 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50678176 50678275 50678176 ID=MS.gene050057.t1.exon3;Parent=MS.gene050057.t1
chr5.4 CDS 50678176 50678275 50678176 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50678371 50678512 50678371 ID=MS.gene050057.t1.exon4;Parent=MS.gene050057.t1
chr5.4 CDS 50678371 50678512 50678371 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50678787 50678850 50678787 ID=MS.gene050057.t1.exon5;Parent=MS.gene050057.t1
chr5.4 CDS 50678787 50678850 50678787 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50678933 50679671 50678933 ID=MS.gene050057.t1.exon6;Parent=MS.gene050057.t1
chr5.4 CDS 50678933 50679671 50678933 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50679932 50680261 50679932 ID=MS.gene050057.t1.exon7;Parent=MS.gene050057.t1
chr5.4 CDS 50679932 50680261 50679932 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50680848 50681129 50680848 ID=MS.gene050057.t1.exon8;Parent=MS.gene050057.t1
chr5.4 CDS 50680848 50681129 50680848 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50682391 50682463 50682391 ID=MS.gene050057.t1.exon9;Parent=MS.gene050057.t1
chr5.4 CDS 50682391 50682463 50682391 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50682565 50682650 50682565 ID=MS.gene050057.t1.exon10;Parent=MS.gene050057.t1
chr5.4 CDS 50682565 50682650 50682565 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50683802 50683921 50683802 ID=MS.gene050057.t1.exon11;Parent=MS.gene050057.t1
chr5.4 CDS 50683802 50683921 50683802 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
chr5.4 exon 50684325 50684393 50684325 ID=MS.gene050057.t1.exon12;Parent=MS.gene050057.t1
chr5.4 CDS 50684325 50684393 50684325 ID=cds.MS.gene050057.t1;Parent=MS.gene050057.t1
Gene Sequence

>MS.gene050057

ATGGCGTTTGCGAGGGAACAAGAAGACAACAACAACACATTCACGACGGGGATTAACAAGCCTCCTATCCATTATGTCTATTCTAGAAAGAGACTCAGAACAAACGCGGGAGCGGTGGCGGAGGAGTTAGCTAAGAACGAAGAACAACAACAACAACAAACCACCGGTAAACGTAACCGGAAAACCGATGAAGCTGCTTCTTCTTCCGTTGCTGTTCGAAAGAGTCAACGGATAAGAAGAACAACAATAAAGGCTCCCGTCTTGGTAGACAAGAAGAAAAAGAAGAAGAAGAAGAATCTTGGTGATGGTGATGGTGCTGAGAAAACCAACACAATTTCTCGAAAGAAACCCGGAAAAAGAAAACAGACTCCTCACAATAAGGTCACAACATCTTCTTCTAGTTATTATTATTATTATTATTATTATTATGCACTGTGGTTTTGTAATAACCTATTAATCACATACATAATTTGAGAAAGTAATATTTCAATTTTTATTTTATTTCGAGAAAATGAAGTAATTCCATGTAATTGTATGTGTTGGTGTCGGACATTGATGCGTGTCTGACACTGGACACTCCTAATCTGATGAGTGACTAACTAACTATTCAATAAACAGTGGATTGAAGAGGAATCGTTAATGTGTCATCAATGTCAAAGAAATGACAGTGGCAGGGTTGTCAGATGCACCAAATGCAAACGGAAAAGATATTGTGTATGCTGTATAAAAAAATGGTATGTTTCTATGTTTCTACAACTTGATTCATTCACCATGTAATGTAATCTAATTTATATCGTTTTTGTTGCTAAGGTATCCTCGATTGAAAGAGGAACAACTTGCTGAGGCATGTCCTGTCTGCTGTGGTAACTGCAATTGCAAAGCTTGTTTGAAGTCACGGAAACTAATCGATGTATGTACTAATCTAAACTTAACTTCTACCTCTGCTATCTACATTATATATATATTCATTTATTAACATTTTAAATCATATTTTATTCACTACAGTCAATTAAAGAAAAGGAGGAGGAAACCAACGATCATCATCAGGTTGAGCGCTCCAAGTATATACTCAAAGCTCTTCTTCCACATTTAATACGATTGGATCAAGATCAAATGGCTGAGAAGGAGATAGAAGCCAAGATACAAGGTATTTTTTTTTCCTCTCTTCTTGCTTTTTTGTCTATCCTCTTATTTATGTCATTTGCTTTTTCCATGGTTCACATTTTATATATACCTTTCTTTCTTCTCGATAACTTACATACTAATTCATATCCTGCTTTACACTATTTTTCCTGCATGTGCTTTTTGTTTATGTGTTACGTTTGTATTTCATCTCATGTGTCGGTCTGTCCGTGATCGATTAGTTTTTAGTTTCATATTATTTTCCATTTTTTAATTGGATCTTGGGATCAACGTTGCAGGTCTCTCACTCTCTGAGCTAAAAATTAAAAAATCAAATCCCCATAATGATGAGCGCATGTACTGGTAGGGACTTATTTGGTATATAAATTGGATTTTTAGTTAGTTATTCCAATGCTACATTGTTAATGACTTATTACTTTTGTAGTGACAACTGCAAAACATCAATATTTGACTACCATAGAAGCTGCACAGAATGCTCTTTTGACCTTTGTCTCCTCTGTTGCCGTGAGCTTCGTTGTGGGCAGCTTCTAGGTGGGGCAGATCCATTTGATTTTGAGTTTATCTTCCGAGGCCCAAATTATCTGCATGGTGAAGTTGCCAAGAAAGTAACCAGATACAAAGCACCTGATGCTGGTGCCCAGCCTGAGATTCCTACATGGTCAAAATCTGGGTGGCATGCTGATAGTGACGGCAATATTCCCTGTCCAAAACCTGAGATTAATTGTGATCACGGTTACCTTGAACTGAAAAGCGTATTTTCGCCAGAATGCATCTCTGAGTTAGTATGTAAAGTAAAGGAACTTGCAGATTCAATGAAACTTCAAGATGCAGAGGTGACTCTTGATAATAGCTGCTCTTGTTTGAATCCTGTTAGGAATAGAGATGATATACACAATAACACAAGGGAAGCAGCTCTTTGCAAAGAATCCAGGGGCAACTTTTTGTACTGTCCTAGGGCTGTAGATCTTCATCACGATGATTTAGGACATTTTCAATGGCACTGGAGCAAAGGGGAGCCTGTAATTGTCAGCAATGTACTTGAATGTACATCTGGTTTAAGCTGGGAGCCGTTTGTCATGTGGCGTGCATTTCGTCAGATAAACAAAAACAAGAACAAGTCACTTTTGGATGTTAAGGCACTTGATTGCTTAGATTGGTGCGAGGTTTGTTCAATTTCTCAATCTTGAACATAAATTTTGTTTGATTTCGTGATCTTTGAGCTTCTCATGCGGTTTCTTGCACTCTAGTGTCCCTTGCATCCCCTGCTTTCTGTTTTTCTGTTGGTATTTTTCTCTGTTTCTTATGCATACTCACCCCAGTTGTGTTCATTTTCTTTCATTTCTGCATTTTTTTTACTATACTTGACATGCCTATAAGTTGCTTGTAACTTCTAAATTTTATTTTAGTTTTTGATTCTATGCAGATAGATATTAATGTGCACCAGTTCTTTACTGGGTACACAAATTGGCCCAAGGATAAACAGAATTGGCCTCAAGTATTGAAATTGAAAGATTGGCCTCCTTCTAAACTATTTGAAGAAAGTTTACCGCGTCATTGTGCGGAGTTTATATCTTCCTTGCCATTTAAAGAATATACAAATCCTTTCAAAGGTGCTCTTAACCTTGCTGTGAAGCTGCCTGATGAAGTTCTAAAGCCAGACATGGGACCAAAAACATATATTGCTTATGGATTTGCTCAGGAGCTTGGACGGGGTGATTCAGTGACTAGGCTCCATTGTGATATGTCCGATGCAGTATGCTTAAATTCTCTGAGTTTTTCCTGGACTGTTTTTTCTAGAATGTCGTGTGTATTTTTTTTATGTTTATGTTGTTATTTATTATTTGAGTATCATATTAAAAGGTGCATAATTTGTAGGATGTATACGATGCCTTTGTAGGCCATTGCATTAAGAGCTGATAGTTGTGTAGTTTCAAGTACATCATATCTTAAGTTTTTGGTCATTGCTCTGTCTTTGAAACACTCATTTGATTATATATTTGATTTGAAATGCTATTTACCTTGGTATTTTGGTAATTGAAAATTGGATAGTATTATTTTTCTGATTAGTTGCATCATTAGATAAGGTATTTTTTGGTCTTGAAATATGAAAGATGGTTTTGGATTAGAATGTTTTACTCGATGTCTACTTCCATCAGATGCATTGTCTTTCCGGATTAATTATTTGTTTGTGCTTTATATGTTTTGCTTTGCGTTTGAGTTCTTACTTTTGTTTGTGCTTTATATGTTTTACTTTGCGTCTTCAGTTCTTTACTCTATTATGAGGAAAATTAAAGTGTTTTTTTTATTTATTTATTTTTTTTTTTTTTTTTGGCTTTTAGGTAAATGTGTTGACTCATATTGCTGAAGCAAAACTGGATCGTGGGAGTATTGACGCAATTAAGAAATTGAAACAAAAGCACCTTGAGCAAGACAAGAGGGAGCTACATGGTGATATTCAGGATGGAGAGACCACTGTTGAAAATAGTTTGTTGGTTGGTGGGGATTCCTTAGATGGTGCTCTTTGGGATATTTTTCGGAGAGAGGACGTACCTGCATTGCAGGAATATCTGAAGAAGCATTTCAGAGAGTTCAGGCATGTCCATTGCTCTCCTGTAAAGGAGGTAAAGTTAGTTGTTATAATTTTCTATCATCTGTTTGGATACCCCCTGTTAACTGGAGTCATTAATGAAACTTAGTTGCTACCGATTATTTTTGCTGCTGTGAACATGCTCTATAATTACCCTTTTATTTTTCACATTTTGCTGCCACGGACATGATAAAGTTGACACTATCTCATTGCTTTTCCCTATTTGACTTAATTCTTAGCTTATGCTTTATTTTATTTTTCTATGTTGTTATTGTTGGTTTGCTTATGTACCTTGTAATATGTTCTATGGGGCCTTCATAGTTTAGTCTCACCTAGCTTTGAAGGATGTAGCACACTCTGTCGTAGTTACAACTGTCTCTAACAATTTAACTCCCTATAGATTCCTATCAGTAATTTAAGTTACATCAAGTAGTTTAGTTTTCAAACAAAAGAAATAAAAAATGCCCATAATGAAGGTATCCTGCAGTGCTGGTCTGGGTTTGTCTAGGCATCGCATTGCCTATTGCCTGCGGCGTTGATTCTTTCTACCTTGCCCACTCTTTTTGTAGAGACCTTGTGCCTCTCTCTCTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTTTTTTTCCTTCTGTCTACCCAAATCTCTCTTTTTATAGGGTTATGTTTGTTATACTACTGTACATTGTAATATGTTGTATTGGTCCTTGGTTGTTCAGTCCTGCAACACATTGGTAAGTTCTGCCTAGCTTAAAAGGATCCAGCACACTTTGTTGGGGGAAACTGTCCATAACAGATTGCACTTCCCTATAAATCATTTTCGTACTTTCTGTTTTGCTAATAAGCTACATCAACAGAAAGTTATCTCAATTATCTGTTTACATTTTCCTTCATTATCTCTTGTTTTCTCAGCTAGTGATTACTTACATCAGTTAGGTTCAGATTTTTTTTTTATTATGAAATTTACATGCCTCGAATGATCAAGCTGGTAATAAATAATATTTGTGAGTAGTTAGTTTTGAATACGGACCATCATGAGTATGGAATTTTGTTGATGCGAATAGCGGTATTGAAACATATGATATGAAGGGAAATGCATTATGGATGTAATTTCCAGTCCCTTAGGTCACCGATTCAGTAGATGTTCTTTCATTTCATTTTACTGTTTCAATTTCAAAGTTCACTTGGCTGTAGACGGGGCTTCTTTATGTAGATGACTCGGCAGTCTGATGACGGTTCATTCATTATGTATGTATCTTTTTGAATGACATCTCCTTTGGCATTATAGGTTATTCACCCCATCCATGATCAGACCTTTTATCTAACCATCGGGCATAAGAAAAAGCTGAAGGAAGAGTATGGTTAGTAGTGCTTATATTTTACTTATTTTGTTTGATTTGTGGAGTGTAGATCAATAGGGTTGTACCATTTTGTTAACAATTCAACACTGACTTGTATGTAGGTATTGAGCCTTGGACTTTTGTTCAGAAGCTTGGAGATGCAGTTTTCATTCCAGCTGGTTGCCCCCACCAAGTCCGAAATCTGAAGGTAAAACTTGCGTACATGGTGGATTTGTTTTATAATGAAGGCATACATTTTCCTTGTTTGCTAAGAAGTGTAGATGTTGATGTTATGAGTTTTTCAAATTTTTTTTTGAGTAACGTTATGAGTTTTTATATAGAAGTAGAGAAAAAATAAATATAAATATGGTTAAAGATGATTGATAATGACTTGATGACTATTATGTTGCCAAGTTCTTGAAAGTTTGAGAATGTCCTAGTAGTTGTATACTGGTATTGTATTCAAAATCCTTGTTGAATTAGTGCTAAATAATTGTCATTTGACAATAATGTGATGTTAACTTGATACAAAAGACTAAATTCTAATTCCTAGGTAGAACATTATTACAACAAGGTCCTAGGTAAAAAATAACTTCCATATTCTGTTATACTTCTTATTATAGAACACTATAATAGGAGATTTCTTAGTTAGTTGGTTATATGCAGTTATGTTTTTATTATTATCTGTGTGTGTCGGTTATGTAAAATGGGAAAGACATGAAAGAATTGGTTATATGGAAGTTAATTAAGTGGTTATTGGGACTCAAAATCATGGAGAACCAGCATAACCCAGCCCTGTTAAGTGTGTTCCTGTTCTGTCTTGTCTTGTAACAATCTCTGTGTTAATAATGTATATGTGTTTATATGAATCCCTTCTCCTAAGGTTAGATCAATAATATTCTATTGTATTTCATAAATAATTATACTTGTACCAGTTTGTATCATATTCCTATTTAAACATTCCTAATGGGGGAGGGGTTTGGGATTTGGTTCCTATGGATGTGAGCATAGAATTTAGCTTGAGCCTAATCAAATCACAAAACTAGCTTATAAGTGAGAGATGCTTCCCACATAAATACTTCAAGCCATATCATCTAGACGTCTAGATCGTTACTCGAGTAGGTTGACAATTGTAGCCAAATGGATATAGCATAGCCTCTGATATCTTAGTATTTACCAATGAGTCACTGAAGTTAATGTTGAGTGCATCTAAGTTCTTTTTGAAGACTGCATTTTTAATTTTTTTTTTATACATATCAGTGTGGAGATTTGAACCTACAATTTCTTACAGATTACCCAATCCATTACTCACTAGCTCACTTTATGTTCTTGCTAATGATATGCATCATCATTTTTCAGTCATGCACCAAGGTTGCTCTTGATTTTGTCTCTCCTGAAAATGTTGGTGAGTGCTTCCGTTTGACAGAGGAATTCCGCCAACTTCCAGTAAATCACAGGTCCATAGAGGACAAATTGGAGGTATGCCTGACTATTATGGATTTGATTATTCTGTTTAGGGTTGATTAGGAGCTTTCAGTTACACCTATTTTTGGCACTGTCATGTTGCCATTGTTCAAATCTCTGAACATTTGTTGGTGGAACTGTTTCAAGCATATTATTCACATTTGTTTGAATTGATAATTTTTTACCATATAGAATCTAGTAAAGAGTATTTGTTATAGATTTTATATTTTCCTATCTAGTAGTATTGTAGTTTTAATGTAAAGACAAGGTTCTAATATTTAACTAATGTTTGGCAGTTTTGAGTGTAAAGCTATTTCAACTAACTATGTTACTTTGTCATACCATCATGTCATATCAACAGATTATTTTTGGTATTTTCTGTATAGTTTGTTTCTTATTGCTGCTTCTTTTGTAACAGGTGAAGAAAATGATCGTATATGCTATGATTGATGTGGTCAAAAAGTTGGAGAAAGCAAGGAGCCAATGA
Protein sequence

>MS.gene050057.t1

MAFAREQEDNNNTFTTGINKPPIHYVYSRKRLRTNAGAVAEELAKNEEQQQQQTTGKRNRKTDEAASSSVAVRKSQRIRRTTIKAPVLVDKKKKKKKKNLGDGDGAEKTNTISRKKPGKRKQTPHNKWIEEESLMCHQCQRNDSGRVVRCTKCKRKRYCVCCIKKWYPRLKEEQLAEACPVCCGNCNCKACLKSRKLIDSIKEKEEETNDHHQVERSKYILKALLPHLIRLDQDQMAEKEIEAKIQGLSLSELKIKKSNPHNDERMYCDNCKTSIFDYHRSCTECSFDLCLLCCRELRCGQLLGGADPFDFEFIFRGPNYLHGEVAKKVTRYKAPDAGAQPEIPTWSKSGWHADSDGNIPCPKPEINCDHGYLELKSVFSPECISELVCKVKELADSMKLQDAEVTLDNSCSCLNPVRNRDDIHNNTREAALCKESRGNFLYCPRAVDLHHDDLGHFQWHWSKGEPVIVSNVLECTSGLSWEPFVMWRAFRQINKNKNKSLLDVKALDCLDWCEIDINVHQFFTGYTNWPKDKQNWPQVLKLKDWPPSKLFEESLPRHCAEFISSLPFKEYTNPFKGALNLAVKLPDEVLKPDMGPKTYIAYGFAQELGRGDSVTRLHCDMSDAVNVLTHIAEAKLDRGSIDAIKKLKQKHLEQDKRELHGDIQDGETTVENSLLVGGDSLDGALWDIFRREDVPALQEYLKKHFREFRHVHCSPVKEVIHPIHDQTFYLTIGHKKKLKEEYGIEPWTFVQKLGDAVFIPAGCPHQVRNLKSCTKVALDFVSPENVGECFRLTEEFRQLPVNHRSIEDKLEVKKMIVYAMIDVVKKLEKARSQ