Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050182.t1 | RHN45574.1 | 73.8 | 210 | 19 | 1 | 1 | 174 | 330 | 539 | 1.50E-80 | 308.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050182.t1 | O82175 | 38.0 | 205 | 85 | 4 | 3 | 174 | 599 | 794 | 6.4e-32 | 138.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050182.t1 | A0A396H1J2 | 73.8 | 210 | 19 | 1 | 1 | 174 | 330 | 539 | 1.1e-80 | 308.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene050182.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050182.t1 | MTR_7g445710 | 73.934 | 211 | 18 | 2 | 1 | 174 | 333 | 543 | 2.59e-103 | 307 |
| MS.gene050182.t1 | MTR_7g445530 | 69.613 | 181 | 18 | 2 | 1 | 144 | 333 | 513 | 1.58e-79 | 245 |
| MS.gene050182.t1 | MTR_7g450730 | 59.524 | 210 | 24 | 2 | 1 | 174 | 321 | 505 | 2.47e-74 | 231 |
| MS.gene050182.t1 | MTR_5g013420 | 36.975 | 238 | 71 | 5 | 2 | 174 | 866 | 1089 | 2.15e-38 | 139 |
| MS.gene050182.t1 | MTR_5g013420 | 36.975 | 238 | 71 | 5 | 2 | 174 | 866 | 1089 | 3.72e-37 | 135 |
| MS.gene050182.t1 | MTR_4g010830 | 35.294 | 238 | 75 | 5 | 2 | 174 | 757 | 980 | 1.08e-35 | 131 |
| MS.gene050182.t1 | MTR_7g445600 | 54.887 | 133 | 45 | 3 | 12 | 144 | 239 | 356 | 3.41e-33 | 120 |
| MS.gene050182.t1 | MTR_4g010550 | 34.742 | 213 | 94 | 6 | 2 | 174 | 257 | 464 | 4.92e-24 | 97.8 |
| MS.gene050182.t1 | MTR_7g084090 | 29.048 | 210 | 98 | 6 | 3 | 174 | 561 | 757 | 2.66e-20 | 87.4 |
| MS.gene050182.t1 | MTR_8g078490 | 30.814 | 172 | 99 | 6 | 15 | 174 | 1090 | 1253 | 1.72e-17 | 79.3 |
| MS.gene050182.t1 | MTR_4g132610 | 32.044 | 181 | 85 | 8 | 15 | 174 | 884 | 1047 | 8.19e-17 | 77.4 |
| MS.gene050182.t1 | MTR_3g011440 | 30.645 | 186 | 94 | 9 | 15 | 169 | 478 | 659 | 5.48e-16 | 75.1 |
| MS.gene050182.t1 | MTR_7g088370 | 30.286 | 175 | 92 | 8 | 15 | 169 | 536 | 700 | 5.65e-16 | 75.1 |
| MS.gene050182.t1 | MTR_8g042750 | 32.065 | 184 | 93 | 9 | 15 | 169 | 503 | 683 | 1.11e-15 | 74.3 |
| MS.gene050182.t1 | MTR_1g048950 | 29.825 | 171 | 94 | 6 | 13 | 174 | 380 | 533 | 1.58e-14 | 70.9 |
| MS.gene050182.t1 | MTR_6g061200 | 26.738 | 187 | 103 | 6 | 3 | 174 | 498 | 665 | 6.40e-14 | 68.9 |
| MS.gene050182.t1 | MTR_5g018850 | 28.729 | 181 | 91 | 8 | 15 | 174 | 1344 | 1507 | 9.46e-14 | 68.6 |
| MS.gene050182.t1 | MTR_8g071030 | 28.244 | 131 | 64 | 2 | 3 | 104 | 544 | 673 | 2.20e-11 | 61.6 |
| MS.gene050182.t1 | MTR_8g078505 | 26.946 | 167 | 99 | 6 | 15 | 169 | 378 | 533 | 3.72e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050182.t1 | AT2G35160 | 38.049 | 205 | 85 | 4 | 3 | 174 | 563 | 758 | 1.48e-37 | 137 |
| MS.gene050182.t1 | AT2G35160 | 38.049 | 205 | 85 | 4 | 3 | 174 | 599 | 794 | 1.50e-37 | 137 |
| MS.gene050182.t1 | AT2G35160 | 38.049 | 205 | 85 | 4 | 3 | 174 | 599 | 794 | 1.50e-37 | 137 |
| MS.gene050182.t1 | AT2G35160 | 38.049 | 205 | 85 | 4 | 3 | 174 | 599 | 794 | 1.50e-37 | 137 |
| MS.gene050182.t1 | AT2G35160 | 38.049 | 205 | 85 | 4 | 3 | 174 | 599 | 794 | 1.50e-37 | 137 |
| MS.gene050182.t1 | AT2G22740 | 35.268 | 224 | 93 | 5 | 3 | 174 | 567 | 790 | 1.29e-35 | 131 |
| MS.gene050182.t1 | AT2G22740 | 35.268 | 224 | 93 | 5 | 3 | 174 | 567 | 790 | 1.29e-35 | 131 |
| MS.gene050182.t1 | AT5G04940 | 30.939 | 181 | 97 | 8 | 15 | 169 | 487 | 665 | 4.71e-14 | 69.7 |
| MS.gene050182.t1 | AT5G04940 | 30.939 | 181 | 97 | 8 | 15 | 169 | 487 | 665 | 4.71e-14 | 69.7 |
| MS.gene050182.t1 | AT1G73100 | 28.962 | 183 | 98 | 7 | 15 | 169 | 486 | 664 | 2.31e-13 | 67.4 |
| MS.gene050182.t1 | AT2G24740 | 25.877 | 228 | 92 | 8 | 3 | 169 | 539 | 750 | 3.59e-11 | 61.2 |
| MS.gene050182.t1 | AT2G23740 | 35.632 | 87 | 50 | 2 | 15 | 95 | 1209 | 1295 | 4.08e-11 | 61.2 |
| MS.gene050182.t1 | AT2G23740 | 35.632 | 87 | 50 | 2 | 15 | 95 | 1216 | 1302 | 4.36e-11 | 61.2 |
| MS.gene050182.t1 | AT2G23740 | 35.632 | 87 | 50 | 2 | 15 | 95 | 1216 | 1302 | 4.36e-11 | 61.2 |
| MS.gene050182.t1 | AT2G23740 | 35.632 | 87 | 50 | 2 | 15 | 95 | 1216 | 1302 | 4.36e-11 | 61.2 |
| MS.gene050182.t1 | AT2G23740 | 35.632 | 87 | 50 | 2 | 15 | 95 | 1216 | 1302 | 5.01e-11 | 60.8 |
| MS.gene050182.t1 | AT2G23740 | 35.632 | 87 | 50 | 2 | 15 | 95 | 1216 | 1302 | 5.01e-11 | 60.8 |
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACCACTTGAAGCTTCTA+AGG | 0.221245 | 7.1:+54104890 | None:intergenic |
| ACCATTGATGCTGCTAAGTT+TGG | 0.302862 | 7.1:-54104831 | MS.gene050182:intron |
| TCAGAGAATGCAATTCCTTT+TGG | 0.305406 | 7.1:-54105083 | MS.gene050182:CDS |
| TTCATATATGGTTGATAGTA+AGG | 0.403159 | 7.1:-54104931 | MS.gene050182:CDS |
| ATTATATTCACAAGGTCTAT+AGG | 0.407539 | 7.1:-54104968 | MS.gene050182:CDS |
| GTGGGAAGAGGATTCATATA+TGG | 0.450043 | 7.1:-54104943 | MS.gene050182:CDS |
| GGTCTATAGGTCGTGGGAAG+AGG | 0.454401 | 7.1:-54104955 | MS.gene050182:CDS |
| GATCAAGTGATTGATTCAAA+TGG | 0.480841 | 7.1:-54104649 | MS.gene050182:CDS |
| TCAAGACTAAAGCGAAAGGA+TGG | 0.483022 | 7.1:-54105115 | MS.gene050182:CDS |
| ACCAAACTTAGCAGCATCAA+TGG | 0.491542 | 7.1:+54104830 | None:intergenic |
| AAAGTCAGCTCATTCATGCT+TGG | 0.503473 | 7.1:+54104691 | None:intergenic |
| TTCACAAGGTCTATAGGTCG+TGG | 0.504274 | 7.1:-54104962 | MS.gene050182:CDS |
| AAGACGAAAGCATGTTATTG+TGG | 0.528019 | 7.1:-54104619 | MS.gene050182:CDS |
| TCACAAATAAAACTTCCAAA+AGG | 0.534102 | 7.1:+54105068 | None:intergenic |
| CAAGACTAAAGCGAAAGGAT+GGG | 0.536026 | 7.1:-54105114 | MS.gene050182:CDS |
| AGAAGCTTCAAGTGGTAAAG+AGG | 0.540440 | 7.1:-54104886 | MS.gene050182:CDS |
| TTACCACTTGAAGCTTCTAA+GGG | 0.542917 | 7.1:+54104891 | None:intergenic |
| CCCTCCACTCTTAAGAGCAC+AGG | 0.546581 | 7.1:+54105187 | None:intergenic |
| AGGTGAGATTATTAAAGATG+AGG | 0.556261 | 7.1:-54105039 | MS.gene050182:CDS |
| ATTGATGATCGATGTGATAG+TGG | 0.558469 | 7.1:-54104861 | MS.gene050182:CDS |
| TCCTGTGCTCTTAAGAGTGG+AGG | 0.560747 | 7.1:-54105188 | MS.gene050182:CDS |
| ACTATCACATCGATCATCAA+TGG | 0.565161 | 7.1:+54104863 | None:intergenic |
| AAGACTAAAGCGAAAGGATG+GGG | 0.572218 | 7.1:-54105113 | MS.gene050182:CDS |
| TGTTCCTGTGCTCTTAAGAG+TGG | 0.599372 | 7.1:-54105191 | MS.gene050182:CDS |
| CCTGTGCTCTTAAGAGTGGA+GGG | 0.600581 | 7.1:-54105187 | MS.gene050182:CDS |
| AATCTCATCAATACAGTCGA+CGG | 0.610597 | 7.1:+54104581 | None:intergenic |
| ATGATCGATGTGATAGTGGT+AGG | 0.614728 | 7.1:-54104857 | MS.gene050182:CDS |
| TCACAAGGTCTATAGGTCGT+GGG | 0.621148 | 7.1:-54104961 | MS.gene050182:CDS |
| TGTTAGCAATTATATTCACA+AGG | 0.626162 | 7.1:-54104976 | MS.gene050182:CDS |
| CAACCCTTAGAAGCTTCAAG+TGG | 0.647600 | 7.1:-54104894 | MS.gene050182:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTTTATTTGTGAGTATTT+AGG | - | chr7.1:54104726-54104745 | MS.gene050182:intron | 20.0% |
| ! | ATTATATTCACAAGGTCTAT+AGG | - | chr7.1:54104817-54104836 | MS.gene050182:intron | 25.0% |
| ! | TCACAAATAAAACTTCCAAA+AGG | + | chr7.1:54104720-54104739 | None:intergenic | 25.0% |
| ! | TGTTAGCAATTATATTCACA+AGG | - | chr7.1:54104809-54104828 | MS.gene050182:intron | 25.0% |
| !! | ATCTGATTTTTCTTTACTCT+AGG | + | chr7.1:54104630-54104649 | None:intergenic | 25.0% |
| !!! | TTCATATATGGTTGATAGTA+AGG | - | chr7.1:54104854-54104873 | MS.gene050182:CDS | 25.0% |
| AACAAATGAGAGAACAACTA+TGG | + | chr7.1:54105001-54105020 | None:intergenic | 30.0% | |
| AGGTGAGATTATTAAAGATG+AGG | - | chr7.1:54104746-54104765 | MS.gene050182:intron | 30.0% | |
| ! | GATCAAGTGATTGATTCAAA+TGG | - | chr7.1:54105136-54105155 | MS.gene050182:CDS | 30.0% |
| !! | ATTTTCAAGACTAAAGCGAA+AGG | - | chr7.1:54104666-54104685 | MS.gene050182:CDS | 30.0% |
| ACTATCACATCGATCATCAA+TGG | + | chr7.1:54104925-54104944 | None:intergenic | 35.0% | |
| TTACCACTTGAAGCTTCTAA+GGG | + | chr7.1:54104897-54104916 | None:intergenic | 35.0% | |
| TTTACCACTTGAAGCTTCTA+AGG | + | chr7.1:54104898-54104917 | None:intergenic | 35.0% | |
| ! | AAGACGAAAGCATGTTATTG+TGG | - | chr7.1:54105166-54105185 | MS.gene050182:CDS | 35.0% |
| ! | ATTGATGATCGATGTGATAG+TGG | - | chr7.1:54104924-54104943 | MS.gene050182:CDS | 35.0% |
| ! | TCAGAGAATGCAATTCCTTT+TGG | - | chr7.1:54104702-54104721 | MS.gene050182:CDS | 35.0% |
| AAAGTCAGCTCATTCATGCT+TGG | + | chr7.1:54105097-54105116 | None:intergenic | 40.0% | |
| AAGACTAAAGCGAAAGGATG+GGG | - | chr7.1:54104672-54104691 | MS.gene050182:CDS | 40.0% | |
| ACCAAACTTAGCAGCATCAA+TGG | + | chr7.1:54104958-54104977 | None:intergenic | 40.0% | |
| ACCATTGATGCTGCTAAGTT+TGG | - | chr7.1:54104954-54104973 | MS.gene050182:CDS | 40.0% | |
| AGAAGCTTCAAGTGGTAAAG+AGG | - | chr7.1:54104899-54104918 | MS.gene050182:CDS | 40.0% | |
| CAAGACTAAAGCGAAAGGAT+GGG | - | chr7.1:54104671-54104690 | MS.gene050182:CDS | 40.0% | |
| GTGGGAAGAGGATTCATATA+TGG | - | chr7.1:54104842-54104861 | MS.gene050182:CDS | 40.0% | |
| TCAAGACTAAAGCGAAAGGA+TGG | - | chr7.1:54104670-54104689 | MS.gene050182:CDS | 40.0% | |
| ! | TTTGCGATGATGATGATCTG+AGG | - | chr7.1:54105035-54105054 | MS.gene050182:CDS | 40.0% |
| !! | ATGATCGATGTGATAGTGGT+AGG | - | chr7.1:54104928-54104947 | MS.gene050182:CDS | 40.0% |
| CAACCCTTAGAAGCTTCAAG+TGG | - | chr7.1:54104891-54104910 | MS.gene050182:CDS | 45.0% | |
| GCTGCATATAGCATGATGTG+TGG | + | chr7.1:54105064-54105083 | None:intergenic | 45.0% | |
| TCACAAGGTCTATAGGTCGT+GGG | - | chr7.1:54104824-54104843 | MS.gene050182:intron | 45.0% | |
| TGTTCCTGTGCTCTTAAGAG+TGG | - | chr7.1:54104594-54104613 | MS.gene050182:CDS | 45.0% | |
| TTCACAAGGTCTATAGGTCG+TGG | - | chr7.1:54104823-54104842 | MS.gene050182:intron | 45.0% | |
| !! | CTAAGTTTGGTAACGTGGCA+AGG | - | chr7.1:54104967-54104986 | MS.gene050182:CDS | 45.0% |
| !! | TGCTGCTAAGTTTGGTAACG+TGG | - | chr7.1:54104962-54104981 | MS.gene050182:CDS | 45.0% |
| CCTGTGCTCTTAAGAGTGGA+GGG | - | chr7.1:54104598-54104617 | MS.gene050182:CDS | 50.0% | |
| TCCTGTGCTCTTAAGAGTGG+AGG | - | chr7.1:54104597-54104616 | MS.gene050182:CDS | 50.0% | |
| GGTCTATAGGTCGTGGGAAG+AGG | - | chr7.1:54104830-54104849 | MS.gene050182:intron | 55.0% | |
| ! | CCCTCCACTCTTAAGAGCAC+AGG | + | chr7.1:54104601-54104620 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 54104588 | 54105219 | 54104588 | ID=MS.gene050182 |
| chr7.1 | mRNA | 54104588 | 54105219 | 54104588 | ID=MS.gene050182.t1;Parent=MS.gene050182 |
| chr7.1 | exon | 54104832 | 54105219 | 54104832 | ID=MS.gene050182.t1.exon1;Parent=MS.gene050182.t1 |
| chr7.1 | CDS | 54104832 | 54105219 | 54104832 | ID=cds.MS.gene050182.t1;Parent=MS.gene050182.t1 |
| chr7.1 | exon | 54104588 | 54104724 | 54104588 | ID=MS.gene050182.t1.exon2;Parent=MS.gene050182.t1 |
| chr7.1 | CDS | 54104588 | 54104724 | 54104588 | ID=cds.MS.gene050182.t1;Parent=MS.gene050182.t1 |
| Gene Sequence |
| Protein sequence |
