AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050186


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050186.t1 MTR_7g450730 69.811 106 26 2 85 190 387 486 2.10e-41 146
MS.gene050186.t1 MTR_7g450730 67.500 80 14 1 1 80 1 68 1.43e-24 100
MS.gene050186.t1 MTR_7g445710 68.269 104 27 2 87 190 427 524 1.28e-39 142
MS.gene050186.t1 MTR_7g445710 77.778 81 9 1 1 81 1 72 3.71e-31 119
MS.gene050186.t1 MTR_7g445530 67.742 93 24 2 87 179 427 513 1.45e-33 125
MS.gene050186.t1 MTR_7g445530 77.778 81 9 1 1 81 1 72 3.06e-31 119
MS.gene050186.t1 MTR_7g445600 50.781 128 49 3 55 179 240 356 3.42e-29 111
MS.gene050186.t1 MTR_7g445600 73.333 75 11 1 7 81 3 68 5.47e-26 103
MS.gene050186.t1 MTR_7g445810 65.957 94 20 1 1 94 1 82 7.35e-29 108
MS.gene050186.t1 MTR_5g013420 64.384 73 24 1 119 191 1001 1071 5.17e-28 110
MS.gene050186.t1 MTR_5g013420 64.384 73 24 1 119 191 1001 1071 5.27e-28 110
MS.gene050186.t1 MTR_4g010830 61.111 72 26 1 119 190 892 961 1.72e-26 106
MS.gene050186.t1 MTR_4g010550 61.111 72 26 1 119 190 376 445 1.29e-25 103
MS.gene050186.t1 MTR_7g084090 46.237 93 46 2 99 191 651 739 1.18e-19 86.7
MS.gene050186.t1 MTR_6g061200 50.685 73 34 1 119 191 577 647 2.23e-19 85.9
MS.gene050186.t1 MTR_7g052480 46.575 73 37 1 119 191 468 538 5.48e-18 82.0
MS.gene050186.t1 MTR_6g061270 48.052 77 38 1 119 195 606 680 4.19e-17 79.3
MS.gene050186.t1 MTR_1g048950 48.649 74 36 1 106 179 437 508 4.77e-17 79.3
MS.gene050186.t1 MTR_8g078490 50.000 66 31 1 117 182 1170 1233 5.13e-14 70.5
MS.gene050186.t1 MTR_8g078505 48.438 64 31 1 118 181 457 518 6.03e-12 64.3
MS.gene050186.t1 MTR_5g018850 44.118 68 36 1 115 182 1422 1487 4.83e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050186.t1 AT2G35160 57.692 78 31 1 113 190 664 739 5.11e-26 105
MS.gene050186.t1 AT2G35160 57.692 78 31 1 113 190 700 775 5.47e-26 105
MS.gene050186.t1 AT2G35160 57.692 78 31 1 113 190 700 775 5.47e-26 105
MS.gene050186.t1 AT2G35160 57.692 78 31 1 113 190 700 775 5.47e-26 105
MS.gene050186.t1 AT2G35160 57.692 78 31 1 113 190 700 775 5.47e-26 105
MS.gene050186.t1 AT2G22740 57.692 78 31 1 113 190 696 771 2.50e-24 100
MS.gene050186.t1 AT2G22740 57.692 78 31 1 113 190 696 771 2.50e-24 100
MS.gene050186.t1 AT5G13960 47.945 73 36 1 119 191 536 606 9.88e-17 78.2
MS.gene050186.t1 AT5G13960 47.945 73 36 1 119 191 402 472 2.14e-16 77.4

Find 32 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCATTGATGCTGCTAAGTA+TGG 0.290913 7.1:+53994406 MS.gene050186:CDS
TTCGACCTTACTATCGTTGA+TGG 0.298068 7.1:-53994320 None:intergenic
ATTGATCTAAATGAAGCATT+TGG 0.313022 7.1:-53995618 None:intergenic
ATTGAAATGGTTTGTGTTGT+GGG 0.345735 7.1:-53994084 None:intergenic
TTGATCTAAATGAAGCATTT+GGG 0.404461 7.1:-53995617 None:intergenic
TTCTTGTTGGAGAATTGAAA+TGG 0.448714 7.1:-53994097 None:intergenic
TTGTGGGTTGCTAGTGGAAG+AGG 0.452053 7.1:-53994068 None:intergenic
ACAACAATGCTCTTTCAGTA+AGG 0.473376 7.1:-53994120 None:intergenic
AGAAACCATCAACGATAGTA+AGG 0.474034 7.1:+53994315 MS.gene050186:CDS
AATCAAGTGACTGATTCAAA+TGG 0.475671 7.1:+53994595 MS.gene050186:CDS
TCTTTCAGTAAGGTTCTTGT+TGG 0.484073 7.1:-53994110 None:intergenic
ACCATACTTAGCAGCATCAA+TGG 0.484758 7.1:-53994407 None:intergenic
AATTGAAATGGTTTGTGTTG+TGG 0.503108 7.1:-53994085 None:intergenic
CCAAGAAACTGAAATTAAAA+CGG 0.505520 7.1:+53994239 MS.gene050186:CDS
TGTCCGTAGCTGCATATAGC+AGG 0.529365 7.1:-53994528 None:intergenic
CGTTACGAAGATGTAACTTC+AGG 0.534855 7.1:+53994262 MS.gene050186:CDS
TGTGGTAGTGGCAGTGATAG+TGG 0.538029 7.1:+53994376 MS.gene050186:CDS
TGGGTTGCTAGTGGAAGAGG+AGG 0.544716 7.1:-53994065 None:intergenic
ATTGATGATCGATGTGGTAG+TGG 0.546467 7.1:+53994364 MS.gene050186:CDS
CATCCTGCTATATGCAGCTA+CGG 0.552116 7.1:+53994525 MS.gene050186:CDS
TTTGTGTTGTGGGTTGCTAG+TGG 0.561230 7.1:-53994074 None:intergenic
CGAAGCTTCAAGTTGTAAAG+AGG 0.564368 7.1:+53994339 MS.gene050186:CDS
ACAAAGAAACACTGAAACCA+AGG 0.572586 7.1:+53994180 MS.gene050186:CDS
CTAAGTATGGTAACATGGCA+AGG 0.577841 7.1:+53994419 MS.gene050186:CDS
GGGTTGCTAGTGGAAGAGGA+GGG 0.633277 7.1:-53994064 None:intergenic
CACAAGGAGAGTATTGTCAA+AGG 0.635793 7.1:+53994156 MS.gene050186:CDS
GTAGTGGCAGTGATAGTGGT+AGG 0.642669 7.1:+53994380 MS.gene050186:CDS
TGTTCAAGAATGTCAGCACA+AGG 0.652837 7.1:+53994140 MS.gene050186:CDS
GCTGCATATAGCAGGATGTG+TGG 0.654272 7.1:-53994520 None:intergenic
TGCTGCTAAGTATGGTAACA+TGG 0.662096 7.1:+53994414 MS.gene050186:CDS
GAGGTCATTGATGATCGATG+TGG 0.693873 7.1:+53994358 MS.gene050186:CDS
TGTACGATGATGATGATCTG+AGG 0.719683 7.1:+53994494 MS.gene050186:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCTTAATTATAAATCTAAT+AGG + chr7.1:53995314-53995333 MS.gene050186:intron 10.0%
!!! AAAAATTTAGGTTTATTATA+TGG + chr7.1:53995086-53995105 MS.gene050186:intron 10.0%
!! ATTCAACAAAATCAATATAT+TGG + chr7.1:53995480-53995499 MS.gene050186:intron 15.0%
!! TATTATATGGAAAAAAAATG+AGG + chr7.1:53995099-53995118 MS.gene050186:intron 15.0%
!! AAAAAAAATGAGGAGTAATT+TGG + chr7.1:53995109-53995128 MS.gene050186:intron 20.0%
!! AGTAATTAATACATTTCCTT+TGG + chr7.1:53995523-53995542 MS.gene050186:intron 20.0%
!! TACAAGAAAAATATCATACA+TGG - chr7.1:53995411-53995430 None:intergenic 20.0%
!! TATAATATATGTTGCTACTA+TGG - chr7.1:53995059-53995078 None:intergenic 20.0%
! AACATTTCGAGCAAAAAAAT+TGG - chr7.1:53994467-53994486 None:intergenic 25.0%
! AAGAAAAATATCATACATGG+TGG - chr7.1:53995408-53995427 None:intergenic 25.0%
! ACATTTCGAGCAAAAAAATT+GGG - chr7.1:53994466-53994485 None:intergenic 25.0%
! ATCAAAATTCATTCATGACA+AGG - chr7.1:53995288-53995307 None:intergenic 25.0%
! CCAAGAAACTGAAATTAAAA+CGG + chr7.1:53994239-53994258 MS.gene050186:CDS 25.0%
!! ATTGATCTAAATGAAGCATT+TGG - chr7.1:53995621-53995640 None:intergenic 25.0%
!! TTGATCTAAATGAAGCATTT+GGG - chr7.1:53995620-53995639 None:intergenic 25.0%
!!! AAGGTGATTTTTTTTTCCTT+TGG + chr7.1:53995205-53995224 MS.gene050186:intron 25.0%
!!! TGATGAGATTTTTTCAGAAA+AGG + chr7.1:53995186-53995205 MS.gene050186:intron 25.0%
AAAAATTGGGAGAACAACTA+TGG - chr7.1:53994453-53994472 None:intergenic 30.0%
AGAGGAATGTAAAATACATG+TGG + chr7.1:53995371-53995390 MS.gene050186:intron 30.0%
ATAGACATTTGATATCAGTG+TGG + chr7.1:53994684-53994703 MS.gene050186:intron 30.0%
ATGAGCAAAATAGAATGAGA+GGG - chr7.1:53995231-53995250 None:intergenic 30.0%
CTGATTCAAATGGAAAAGTA+AGG + chr7.1:53994605-53994624 MS.gene050186:intron 30.0%
GGAAACAACAATCAAAAACT+TGG + chr7.1:53994201-53994220 MS.gene050186:CDS 30.0%
TATGAGCAAAATAGAATGAG+AGG - chr7.1:53995232-53995251 None:intergenic 30.0%
TTCCTCTACAACAACTAAAA+CGG - chr7.1:53995358-53995377 None:intergenic 30.0%
TTCTTGTTGGAGAATTGAAA+TGG - chr7.1:53994100-53994119 None:intergenic 30.0%
! AATCAAGTGACTGATTCAAA+TGG + chr7.1:53994595-53994614 MS.gene050186:CDS 30.0%
! AATTGAAATGGTTTGTGTTG+TGG - chr7.1:53994088-53994107 None:intergenic 30.0%
! ATTGAAATGGTTTGTGTTGT+GGG - chr7.1:53994087-53994106 None:intergenic 30.0%
! TAAGTCAGCTCATTCATTTT+CGG - chr7.1:53994556-53994575 None:intergenic 30.0%
!!! CCGTTTTAATTTCAGTTTCT+TGG - chr7.1:53994242-53994261 None:intergenic 30.0%
!!! GTTTTTGATTGTTGTTTCCT+TGG - chr7.1:53994200-53994219 None:intergenic 30.0%
ACAAAGAAACACTGAAACCA+AGG + chr7.1:53994180-53994199 MS.gene050186:CDS 35.0%
ACAACAATGCTCTTTCAGTA+AGG - chr7.1:53994123-53994142 None:intergenic 35.0%
AGAAACCATCAACGATAGTA+AGG + chr7.1:53994315-53994334 MS.gene050186:CDS 35.0%
ATCATACATGGTGGCTTAAT+TGG - chr7.1:53995399-53995418 None:intergenic 35.0%
CTATGGCATGATTGTTCATA+TGG - chr7.1:53995042-53995061 None:intergenic 35.0%
GAATAACATTGCAGTTGCAA+GGG - chr7.1:53995464-53995483 None:intergenic 35.0%
TCATCAATACAGTCTACCAT+GGG - chr7.1:53995172-53995191 None:intergenic 35.0%
TCATGTTCAACTTCATCCAA+AGG - chr7.1:53995542-53995561 None:intergenic 35.0%
TCCTCTACAACAACTAAAAC+GGG - chr7.1:53995357-53995376 None:intergenic 35.0%
TCTTTCAGTAAGGTTCTTGT+TGG - chr7.1:53994113-53994132 None:intergenic 35.0%
TGAATAACATTGCAGTTGCA+AGG - chr7.1:53995465-53995484 None:intergenic 35.0%
! AAATGAGGAGTAATTTGGTG+TGG + chr7.1:53995114-53995133 MS.gene050186:intron 35.0%
! GTTGATGAATGGTTTGTATG+CGG - chr7.1:53995000-53995019 None:intergenic 35.0%
! TTTGAGTTTGCGTTGATGAA+TGG - chr7.1:53995011-53995030 None:intergenic 35.0%
!! AGGAAGAAAGAGTGTTATTG+TGG + chr7.1:53994625-53994644 MS.gene050186:intron 35.0%
!! TAAGCTGAAGCGTTTTAATG+CGG - chr7.1:53994929-53994948 None:intergenic 35.0%
AAATAGAATGAGAGGGCCAA+AGG - chr7.1:53995224-53995243 None:intergenic 40.0%
ACCATACTTAGCAGCATCAA+TGG - chr7.1:53994410-53994429 None:intergenic 40.0%
ACCATTGATGCTGCTAAGTA+TGG + chr7.1:53994406-53994425 MS.gene050186:CDS 40.0%
CAGTCTACGAATGCATTCAA+AGG - chr7.1:53994653-53994672 None:intergenic 40.0%
CCTCTACAACAACTAAAACG+GGG - chr7.1:53995356-53995375 None:intergenic 40.0%
CGTTACGAAGATGTAACTTC+AGG + chr7.1:53994262-53994281 MS.gene050186:CDS 40.0%
CTAAGTATGGTAACATGGCA+AGG + chr7.1:53994419-53994438 MS.gene050186:CDS 40.0%
CTCATCAATACAGTCTACCA+TGG - chr7.1:53995173-53995192 None:intergenic 40.0%
TGATTGTTCATATGGCCTAG+TGG - chr7.1:53995034-53995053 None:intergenic 40.0%
TGCTGCTAAGTATGGTAACA+TGG + chr7.1:53994414-53994433 MS.gene050186:CDS 40.0%
TGTTCAAGAATGTCAGCACA+AGG + chr7.1:53994140-53994159 MS.gene050186:CDS 40.0%
TTCGACCTTACTATCGTTGA+TGG - chr7.1:53994323-53994342 None:intergenic 40.0%
! ATTGATGATCGATGTGGTAG+TGG + chr7.1:53994364-53994383 MS.gene050186:CDS 40.0%
! TGTACGATGATGATGATCTG+AGG + chr7.1:53994494-53994513 MS.gene050186:CDS 40.0%
!! CACAAGGAGAGTATTGTCAA+AGG + chr7.1:53994156-53994175 MS.gene050186:CDS 40.0%
!! CGAAGCTTCAAGTTGTAAAG+AGG + chr7.1:53994339-53994358 MS.gene050186:CDS 40.0%
AATGCATTCGTAGACTGCAG+GGG + chr7.1:53994656-53994675 MS.gene050186:intron 45.0%
ACAACAACTAAAACGGGGCA+TGG - chr7.1:53995351-53995370 None:intergenic 45.0%
ACATTAAAAGGGTTGCCTGC+TGG + chr7.1:53994966-53994985 MS.gene050186:intron 45.0%
ATCCACATTGAAGCATGGTG+TGG - chr7.1:53995146-53995165 None:intergenic 45.0%
CAACGCAAACTCAAACCACT+AGG + chr7.1:53995016-53995035 MS.gene050186:intron 45.0%
CATCCTGCTATATGCAGCTA+CGG + chr7.1:53994525-53994544 MS.gene050186:CDS 45.0%
CATTAAAAGGGTTGCCTGCT+GGG + chr7.1:53994967-53994986 MS.gene050186:intron 45.0%
GAATGCATTCGTAGACTGCA+GGG + chr7.1:53994655-53994674 MS.gene050186:intron 45.0%
TCAATACAGTCTACCATGGG+CGG - chr7.1:53995169-53995188 None:intergenic 45.0%
TGAATGCATTCGTAGACTGC+AGG + chr7.1:53994654-53994673 MS.gene050186:intron 45.0%
! GAGGTCATTGATGATCGATG+TGG + chr7.1:53994358-53994377 MS.gene050186:CDS 45.0%
! TTTGTGTTGTGGGTTGCTAG+TGG - chr7.1:53994077-53994096 None:intergenic 45.0%
!!! CCCCGTTTTAGTTGTTGTAG+AGG + chr7.1:53995353-53995372 MS.gene050186:intron 45.0%
!! ATATTTATAAAAACATTAAA+AGG + chr7.1:53994902-53994921 MS.gene050186:intron 5.0%
!! TATTTATAAAAACATTAAAA+GGG + chr7.1:53994955-53994974 MS.gene050186:intron 5.0%
!! TTATATATAATGAAAAATTT+AGG + chr7.1:53995074-53995093 MS.gene050186:intron 5.0%
GCTGCATATAGCAGGATGTG+TGG - chr7.1:53994523-53994542 None:intergenic 50.0%
TGCCACACCATGCTTCAATG+TGG + chr7.1:53995141-53995160 MS.gene050186:intron 50.0%
TGTCCGTAGCTGCATATAGC+AGG - chr7.1:53994531-53994550 None:intergenic 50.0%
! TTGTGGGTTGCTAGTGGAAG+AGG - chr7.1:53994071-53994090 None:intergenic 50.0%
!! GTAGTGGCAGTGATAGTGGT+AGG + chr7.1:53994380-53994399 MS.gene050186:CDS 50.0%
!! TGTGGTAGTGGCAGTGATAG+TGG + chr7.1:53994376-53994395 MS.gene050186:CDS 50.0%
CTTCAATGTGGATCCGCCCA+TGG + chr7.1:53995153-53995172 MS.gene050186:intron 55.0%
GGCGGATCCACATTGAAGCA+TGG - chr7.1:53995151-53995170 None:intergenic 55.0%
GGGTTGCTAGTGGAAGAGGA+GGG - chr7.1:53994067-53994086 None:intergenic 55.0%
TATGCGGTTCAACACCCAGC+AGG - chr7.1:53994984-53995003 None:intergenic 55.0%
TGGGTTGCTAGTGGAAGAGG+AGG - chr7.1:53994068-53994087 None:intergenic 55.0%
GCATTCGTAGACTGCAGGGG+CGG + chr7.1:53994659-53994678 MS.gene050186:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 53994049 53995645 53994049 ID=MS.gene050186
chr7.1 mRNA 53994049 53995645 53994049 ID=MS.gene050186.t1;Parent=MS.gene050186
chr7.1 exon 53994049 53994621 53994049 ID=MS.gene050186.t1.exon1;Parent=MS.gene050186.t1
chr7.1 CDS 53994049 53994621 53994049 ID=cds.MS.gene050186.t1;Parent=MS.gene050186.t1
chr7.1 exon 53995595 53995645 53995595 ID=MS.gene050186.t1.exon2;Parent=MS.gene050186.t1
chr7.1 CDS 53995595 53995645 53995595 ID=cds.MS.gene050186.t1;Parent=MS.gene050186.t1
Gene Sequence

>MS.gene050186

ATGACTAAGATTAAGCCCTCCTCTTCCACTAGCAACCCACAACACAAACCATTTCAATTCTCCAACAAGAACCTTACTGAAAGAGCATTGTTGTTCAAGAATGTCAGCACAAGGAGAGTATTGTCAAAGGTACAAAGAAACACTGAAACCAAGGAAACAACAATCAAAAACTTGGAAAAGAATGATATATCCAAGAAACTGAAATTAAAACGGCGTTACGAAGATGTAACTTCAGGTAACAAAAAGAACAAAGAGAATGAGAGTAAAGAAACCATCAACGATAGTAAGGTCGAAGCTTCAAGTTGTAAAGAGGTCATTGATGATCGATGTGGTAGTGGCAGTGATAGTGGTAGGTTTACCATTGATGCTGCTAAGTATGGTAACATGGCAAGGTTTATCAACCATAGTTGTTCTCCCAATTTTTTTGCTCGAAATGTTCTGTACATGTACGATGATGATGATCTGAGGATTCCACACATCCTGCTATATGCAGCTACGGACATTCCGAAAATGAATGAGCTGACTTACAACTACAATTACAAGATAAATCAAGTGACTGATTCAAATGGAAAAGTAAGGAAGAAAGAGTGTTATTGTGGAGCCTTTGAATGCATTCGTAGACTGCAGGGGCGGATATAGACATTTGATATCAGTGTGGCAAAAAAATAACTATGAAGCAAATTATATTAATATATATACTAAAAAGATTAAATTTTGACATCTAAGACGCGTCAGCTCTAAAACGCTTCAGCTTATGTTAATATTTATAAAAACATTTATTAATATATATACTAAAAAGATTAAATTTTGACATCTAAGACGCGTCAGCTCTAAAACGCTTCAGCTTATGTTAATATTTATAAAAACATTAAATGAACCGCATTAAAACGCTTCAGCTTATGTTAATATTTATAAAAACATTAAAAGGGTTGCCTGCTGGGTGTTGAACCGCATACAAACCATTCATCAACGCAAACTCAAACCACTAGGCCATATGAACAATCATGCCATAGTAGCAACATATATTATATATAATGAAAAATTTAGGTTTATTATATGGAAAAAAAATGAGGAGTAATTTGGTGTGGCAAGTGCCACACCATGCTTCAATGTGGATCCGCCCATGGTAGACTGTATTGATGAGATTTTTTCAGAAAAGGTGATTTTTTTTTCCTTTGGCCCTCTCATTCTATTTTGCTCATAAATTCATATTCTTCTCTGCTTTATTAAATGCTACCTTGTCATGAATGAATTTTGATGTTGTTTTCTTAATTATAAATCTAATAGGTGATATTCAAGCCATGCCCCGTTTTAGTTGTTGTAGAGGAATGTAAAATACATGTGGAACCAATTAAGCCACCATGTATGATATTTTTCTTGTAGACAAATCTTACTTTATATAGTATTCAGCTCCCTTGCAACTGCAATGTTATTCAACAAAATCAATATATTGGTACAACAGTGTAATTACTTAAGTAATTAATACATTTCCTTTGGATGAAGTTGAACATGACTGTTTAGATTTATGCTAATATATGATTTTCAGTGTATATCAGTACTTCTCATTGCCCAAATGCTTCATTTAGATCAATACTGA

Protein sequence

>MS.gene050186.t1

MTKIKPSSSTSNPQHKPFQFSNKNLTERALLFKNVSTRRVLSKVQRNTETKETTIKNLEKNDISKKLKLKRRYEDVTSGNKKNKENESKETINDSKVEASSCKEVIDDRCGSGSDSGRFTIDAAKYGNMARFINHSCSPNFFARNVLYMYDDDDLRIPHILLYAATDIPKMNELTYNYNYKINQVTDSNGKCISVLLIAQMLHLDQY