Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050262.t1 | RHN67051.1 | 81.9 | 155 | 27 | 1 | 1 | 154 | 1 | 155 | 4.20E-63 | 250.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050262.t1 | G7J1Z7 | 81.9 | 155 | 27 | 1 | 1 | 154 | 1 | 155 | 3.1e-63 | 250.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene050262.t1 | TR | GNAT |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050262.t1 | MTR_3g050860 | 81.935 | 155 | 27 | 1 | 1 | 154 | 1 | 155 | 1.99e-87 | 259 |
| MS.gene050262.t1 | MTR_0439s0020 | 80.420 | 143 | 26 | 1 | 1 | 141 | 1 | 143 | 1.12e-79 | 232 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 37 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCGTCAGTGTTGGTCTTC+GGG | 0.301799 | 7.1:+52651249 | None:intergenic |
| GGTTTCATCGTCTTTGATAT+TGG | 0.310497 | 7.1:-52651540 | MS.gene050262:CDS |
| AACCTTGTCCAAGTCCATTT+CGG | 0.354048 | 7.1:+52651422 | None:intergenic |
| AATGCGTCAGTGTTGGTCTT+CGG | 0.376239 | 7.1:+52651248 | None:intergenic |
| ACTTGTGACCATGGCCGAAA+TGG | 0.380600 | 7.1:-52651436 | MS.gene050262:CDS |
| CATCCACAAGAATCTTAAAT+AGG | 0.405177 | 7.1:+52651649 | None:intergenic |
| AACCCTATCTTTCGGAGAAA+AGG | 0.415290 | 7.1:-52651471 | MS.gene050262:CDS |
| TCTTCGTTAACCCTATCTTT+CGG | 0.417887 | 7.1:-52651479 | MS.gene050262:CDS |
| GTGAAGAGCAATAATATTGA+TGG | 0.429245 | 7.1:-52651191 | MS.gene050262:CDS |
| AGAATCTTAAATAGGTCAAC+AGG | 0.430459 | 7.1:+52651657 | None:intergenic |
| TGACGTGGATGCTAAAAGTT+TGG | 0.430756 | 7.1:-52651616 | MS.gene050262:CDS |
| GTCCAAGTCCATTTCGGCCA+TGG | 0.446272 | 7.1:+52651428 | None:intergenic |
| GACCATGGCCGAAATGGACT+TGG | 0.499061 | 7.1:-52651430 | MS.gene050262:CDS |
| GATCTGAAGCATACTGGTTA+TGG | 0.504056 | 7.1:-52651516 | MS.gene050262:CDS |
| TGACCTATTTAAGATTCTTG+TGG | 0.505182 | 7.1:-52651652 | MS.gene050262:CDS |
| GAGCAATAATATTGATGGGA+AGG | 0.524325 | 7.1:-52651186 | MS.gene050262:CDS |
| ATTGGTGATCTGAAGCATAC+TGG | 0.528676 | 7.1:-52651522 | MS.gene050262:CDS |
| GTCGCCGAGGATAAACTCCT+AGG | 0.541691 | 7.1:-52651573 | MS.gene050262:CDS |
| GGCCGAAATGGACTTGGACA+AGG | 0.545609 | 7.1:-52651424 | MS.gene050262:intron |
| TGAAGAGCAATAATATTGAT+GGG | 0.562579 | 7.1:-52651190 | MS.gene050262:CDS |
| GCATCTCTTACGACCACATG+TGG | 0.566352 | 7.1:+52651684 | None:intergenic |
| AAACTCCTAGGGTTTATCCT+CGG | 0.580594 | 7.1:-52651561 | MS.gene050262:CDS |
| ATCATGACGAAGAAGCTCGA+GGG | 0.582010 | 7.1:-52651149 | MS.gene050262:CDS |
| CGCTACTCGACTTGTGACCA+TGG | 0.583316 | 7.1:-52651445 | MS.gene050262:CDS |
| TAAGAGTAATGCGTCAGTGT+TGG | 0.594559 | 7.1:+52651241 | None:intergenic |
| TCGCCGAGGATAAACTCCTA+GGG | 0.604901 | 7.1:-52651572 | MS.gene050262:CDS |
| AAACCCTAGGAGTTTATCCT+CGG | 0.607628 | 7.1:+52651569 | None:intergenic |
| GTCAGTGTTGGTCTTCGGGA+CGG | 0.627712 | 7.1:+52651253 | None:intergenic |
| ATCTCTTACGACCACATGTG+GGG | 0.629012 | 7.1:+52651686 | None:intergenic |
| TGAAACCGAGGATAAACCCT+AGG | 0.631949 | 7.1:+52651556 | None:intergenic |
| CATCTCTTACGACCACATGT+GGG | 0.632600 | 7.1:+52651685 | None:intergenic |
| GATGCTAAAAGTTTGGCGAA+TGG | 0.635951 | 7.1:-52651609 | MS.gene050262:CDS |
| CATCATGACGAAGAAGCTCG+AGG | 0.646448 | 7.1:-52651150 | MS.gene050262:CDS |
| TCATGACGAAGAAGCTCGAG+GGG | 0.655307 | 7.1:-52651148 | MS.gene050262:CDS |
| AAAGATGGAGTCCCCACATG+TGG | 0.657747 | 7.1:-52651697 | None:intergenic |
| TATCAAAGACGATGAAACCG+AGG | 0.669702 | 7.1:+52651544 | None:intergenic |
| TAAGATTCTTCAAGTCGCCG+AGG | 0.715340 | 7.1:-52651586 | MS.gene050262:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTGTCTTCTTTTAATTTAA+TGG | - | chr7.1:52651470-52651489 | MS.gene050262:CDS | 20.0% |
| ! | AAAATTATGACAGAGTCAAT+AGG | + | chr7.1:52651437-52651456 | None:intergenic | 25.0% |
| ! | ATTCAAAAAGATGAAGTATG+AGG | - | chr7.1:52651601-52651620 | MS.gene050262:CDS | 25.0% |
| ! | TGAAGAGCAATAATATTGAT+GGG | - | chr7.1:52651630-52651649 | MS.gene050262:CDS | 25.0% |
| !!! | TTTTAATTTAATGGCGTGAT+TGG | - | chr7.1:52651479-52651498 | MS.gene050262:CDS | 25.0% |
| AGAATCTTAAATAGGTCAAC+AGG | + | chr7.1:52651166-52651185 | None:intergenic | 30.0% | |
| CATCCACAAGAATCTTAAAT+AGG | + | chr7.1:52651174-52651193 | None:intergenic | 30.0% | |
| GTGAAGAGCAATAATATTGA+TGG | - | chr7.1:52651629-52651648 | MS.gene050262:CDS | 30.0% | |
| ! | GATTGGTGTAGTTTGTAATA+TGG | - | chr7.1:52651496-52651515 | MS.gene050262:CDS | 30.0% |
| ! | TGACCTATTTAAGATTCTTG+TGG | - | chr7.1:52651168-52651187 | MS.gene050262:CDS | 30.0% |
| CAAAAAGATGAAGTATGAGG+AGG | - | chr7.1:52651604-52651623 | MS.gene050262:CDS | 35.0% | |
| GAGCAATAATATTGATGGGA+AGG | - | chr7.1:52651634-52651653 | MS.gene050262:CDS | 35.0% | |
| GGTTTCATCGTCTTTGATAT+TGG | - | chr7.1:52651280-52651299 | MS.gene050262:CDS | 35.0% | |
| TCTTCGTTAACCCTATCTTT+CGG | - | chr7.1:52651341-52651360 | MS.gene050262:intron | 35.0% | |
| AAACCCTAGGAGTTTATCCT+CGG | + | chr7.1:52651254-52651273 | None:intergenic | 40.0% | |
| AAACTCCTAGGGTTTATCCT+CGG | - | chr7.1:52651259-52651278 | MS.gene050262:CDS | 40.0% | |
| AACCCTATCTTTCGGAGAAA+AGG | - | chr7.1:52651349-52651368 | MS.gene050262:intron | 40.0% | |
| AACCTTGTCCAAGTCCATTT+CGG | + | chr7.1:52651401-52651420 | None:intergenic | 40.0% | |
| CTGTTACTGTGTTTGTAGCA+AGG | - | chr7.1:52651524-52651543 | MS.gene050262:CDS | 40.0% | |
| GATGCTAAAAGTTTGGCGAA+TGG | - | chr7.1:52651211-52651230 | MS.gene050262:CDS | 40.0% | |
| TAAGAGTAATGCGTCAGTGT+TGG | + | chr7.1:52651582-52651601 | None:intergenic | 40.0% | |
| TATCAAAGACGATGAAACCG+AGG | + | chr7.1:52651279-52651298 | None:intergenic | 40.0% | |
| TGACGTGGATGCTAAAAGTT+TGG | - | chr7.1:52651204-52651223 | MS.gene050262:CDS | 40.0% | |
| TGCTACAAACACAGTAACAG+AGG | + | chr7.1:52651524-52651543 | None:intergenic | 40.0% | |
| ! | AAACTTTTAGCATCCACGTC+AGG | + | chr7.1:52651205-52651224 | None:intergenic | 40.0% |
| ! | ATTGGTGATCTGAAGCATAC+TGG | - | chr7.1:52651298-52651317 | MS.gene050262:intron | 40.0% |
| ! | GATCTGAAGCATACTGGTTA+TGG | - | chr7.1:52651304-52651323 | MS.gene050262:intron | 40.0% |
| ! | GTCCTTTTCTCCGAAAGATA+GGG | + | chr7.1:52651354-52651373 | None:intergenic | 40.0% |
| ! | TGTCCTTTTCTCCGAAAGAT+AGG | + | chr7.1:52651355-52651374 | None:intergenic | 40.0% |
| ATCATGACGAAGAAGCTCGA+GGG | - | chr7.1:52651671-52651690 | MS.gene050262:CDS | 45.0% | |
| ATCTCTTACGACCACATGTG+GGG | + | chr7.1:52651137-52651156 | None:intergenic | 45.0% | |
| CATCTCTTACGACCACATGT+GGG | + | chr7.1:52651138-52651157 | None:intergenic | 45.0% | |
| TAAGATTCTTCAAGTCGCCG+AGG | - | chr7.1:52651234-52651253 | MS.gene050262:CDS | 45.0% | |
| TGAAACCGAGGATAAACCCT+AGG | + | chr7.1:52651267-52651286 | None:intergenic | 45.0% | |
| !! | AATGCGTCAGTGTTGGTCTT+CGG | + | chr7.1:52651575-52651594 | None:intergenic | 45.0% |
| ACTTGTGACCATGGCCGAAA+TGG | - | chr7.1:52651384-52651403 | MS.gene050262:intron | 50.0% | |
| CATCATGACGAAGAAGCTCG+AGG | - | chr7.1:52651670-52651689 | MS.gene050262:CDS | 50.0% | |
| GCATCTCTTACGACCACATG+TGG | + | chr7.1:52651139-52651158 | None:intergenic | 50.0% | |
| TCATGACGAAGAAGCTCGAG+GGG | - | chr7.1:52651672-52651691 | MS.gene050262:CDS | 50.0% | |
| TCGCCGAGGATAAACTCCTA+GGG | - | chr7.1:52651248-52651267 | MS.gene050262:CDS | 50.0% | |
| !! | ATGCGTCAGTGTTGGTCTTC+GGG | + | chr7.1:52651574-52651593 | None:intergenic | 50.0% |
| !!! | GGATGCTGTTTTACCTGACG+TGG | - | chr7.1:52651189-52651208 | MS.gene050262:CDS | 50.0% |
| GTCCAAGTCCATTTCGGCCA+TGG | + | chr7.1:52651395-52651414 | None:intergenic | 55.0% | |
| GTCGCCGAGGATAAACTCCT+AGG | - | chr7.1:52651247-52651266 | MS.gene050262:CDS | 55.0% | |
| ! | CGCTACTCGACTTGTGACCA+TGG | - | chr7.1:52651375-52651394 | MS.gene050262:intron | 55.0% |
| ! | GACCATGGCCGAAATGGACT+TGG | - | chr7.1:52651390-52651409 | MS.gene050262:intron | 55.0% |
| ! | GGCCGAAATGGACTTGGACA+AGG | - | chr7.1:52651396-52651415 | MS.gene050262:intron | 55.0% |
| !! | GTCAGTGTTGGTCTTCGGGA+CGG | + | chr7.1:52651570-52651589 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 52651127 | 52651715 | 52651127 | ID=MS.gene050262 |
| chr7.1 | mRNA | 52651127 | 52651715 | 52651127 | ID=MS.gene050262.t1;Parent=MS.gene050262 |
| chr7.1 | exon | 52651425 | 52651715 | 52651425 | ID=MS.gene050262.t1.exon1;Parent=MS.gene050262.t1 |
| chr7.1 | CDS | 52651425 | 52651715 | 52651425 | ID=cds.MS.gene050262.t1;Parent=MS.gene050262.t1 |
| chr7.1 | exon | 52651127 | 52651300 | 52651127 | ID=MS.gene050262.t1.exon2;Parent=MS.gene050262.t1 |
| chr7.1 | CDS | 52651127 | 52651300 | 52651127 | ID=cds.MS.gene050262.t1;Parent=MS.gene050262.t1 |
| Gene Sequence |
| Protein sequence |
