Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050263.t1 | AES78884.2 | 79.2 | 212 | 36 | 2 | 1 | 204 | 82 | 293 | 6.50E-86 | 327 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050263.t1 | Q0JP99 | 36.3 | 135 | 72 | 4 | 77 | 207 | 133 | 257 | 5.5e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050263.t1 | G7KVV7 | 79.2 | 212 | 36 | 2 | 1 | 204 | 82 | 293 | 4.7e-86 | 327.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene050263.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050263.t1 | MTR_7g050590 | 79.245 | 212 | 36 | 2 | 1 | 204 | 82 | 293 | 3.44e-117 | 335 |
| MS.gene050263.t1 | MTR_7g050560 | 69.302 | 215 | 55 | 4 | 1 | 214 | 109 | 313 | 2.34e-100 | 293 |
| MS.gene050263.t1 | MTR_7g050580 | 66.514 | 218 | 54 | 3 | 2 | 214 | 110 | 313 | 2.71e-93 | 275 |
| MS.gene050263.t1 | MTR_7g050710 | 62.911 | 213 | 69 | 5 | 1 | 204 | 109 | 320 | 9.64e-86 | 256 |
| MS.gene050263.t1 | MTR_3g098790 | 63.333 | 210 | 65 | 4 | 1 | 204 | 109 | 312 | 2.01e-85 | 256 |
| MS.gene050263.t1 | MTR_7g050720 | 52.830 | 212 | 81 | 8 | 1 | 193 | 106 | 317 | 9.54e-62 | 195 |
| MS.gene050263.t1 | MTR_1g035460 | 40.625 | 128 | 63 | 5 | 81 | 208 | 188 | 302 | 1.45e-20 | 89.0 |
| MS.gene050263.t1 | MTR_1g108780 | 37.190 | 121 | 63 | 4 | 72 | 191 | 182 | 290 | 1.40e-18 | 83.6 |
| MS.gene050263.t1 | MTR_8g103940 | 34.946 | 186 | 91 | 9 | 22 | 191 | 160 | 331 | 1.56e-16 | 77.8 |
| MS.gene050263.t1 | MTR_1g035490 | 36.290 | 124 | 72 | 4 | 73 | 194 | 81 | 199 | 1.53e-13 | 68.6 |
| MS.gene050263.t1 | MTR_4g068320 | 31.967 | 122 | 77 | 2 | 74 | 195 | 277 | 392 | 1.03e-12 | 66.6 |
| MS.gene050263.t1 | MTR_1g034240 | 29.921 | 127 | 83 | 2 | 65 | 191 | 272 | 392 | 3.91e-12 | 65.1 |
| MS.gene050263.t1 | MTR_5g044670 | 35.156 | 128 | 71 | 4 | 81 | 208 | 199 | 314 | 1.23e-11 | 63.2 |
| MS.gene050263.t1 | MTR_1g021410 | 32.203 | 118 | 72 | 4 | 82 | 196 | 239 | 351 | 9.98e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050263.t1 | AT1G49480 | 30.000 | 170 | 102 | 6 | 23 | 191 | 68 | 221 | 3.24e-13 | 66.6 |
| MS.gene050263.t1 | AT1G49480 | 30.000 | 170 | 102 | 6 | 23 | 191 | 68 | 221 | 3.24e-13 | 66.6 |
| MS.gene050263.t1 | AT1G49480 | 30.000 | 170 | 102 | 6 | 23 | 191 | 68 | 221 | 3.24e-13 | 66.6 |
Find 30 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCATCAACCACGTGAAAT+TGG | 0.219339 | 7.1:+52608082 | MS.gene050263:CDS |
| GGACTAAAATTTCAACTTAC+AGG | 0.245796 | 7.1:+52609093 | MS.gene050263:CDS |
| AGGTGTTAAATGAAGGAGTT+TGG | 0.351103 | 7.1:+52609037 | MS.gene050263:CDS |
| AAGTAGCTTCTTCTTTCATT+TGG | 0.368122 | 7.1:-52609354 | None:intergenic |
| TGGTAATAGACACTTTGATT+TGG | 0.391800 | 7.1:+52608987 | MS.gene050263:CDS |
| CAGAATATACCACGTATGTT+TGG | 0.412403 | 7.1:+52608967 | MS.gene050263:intron |
| ATGAAAGAAGAAGCTACTTA+AGG | 0.444131 | 7.1:+52609358 | MS.gene050263:CDS |
| ACTTTGAGATGAAGAACAAT+TGG | 0.461943 | 7.1:-52609241 | None:intergenic |
| CAAGGAAATAAACGTTCAAA+TGG | 0.463400 | 7.1:+52608013 | MS.gene050263:CDS |
| TCTATCAAGAAGTGCAGTAA+AGG | 0.491677 | 7.1:+52609072 | MS.gene050263:CDS |
| TTTGATTTGGATAAGAAAAG+AGG | 0.493087 | 7.1:+52609000 | MS.gene050263:CDS |
| ACGTTTATTTCCTTGACCAT+TGG | 0.495512 | 7.1:-52608005 | None:intergenic |
| CAACGAGTTTCCAATGGTCA+AGG | 0.497922 | 7.1:+52607995 | MS.gene050263:CDS |
| CTTGACCATTGGAAACTCGT+TGG | 0.499234 | 7.1:-52607994 | None:intergenic |
| GAGGAAAATAACTTGAAAGT+TGG | 0.503659 | 7.1:+52609135 | MS.gene050263:CDS |
| CATTGGAAACTCGTTGGAAA+GGG | 0.505311 | 7.1:-52607988 | None:intergenic |
| ATGAAGGAGTTTGGACTGCA+AGG | 0.527571 | 7.1:+52609046 | MS.gene050263:CDS |
| AATTTCAACTTACAGGTGGA+TGG | 0.530556 | 7.1:+52609100 | MS.gene050263:CDS |
| CCATTGGAAACTCGTTGGAA+AGG | 0.533786 | 7.1:-52607989 | None:intergenic |
| CGCATGACAACAACAAAGGA+TGG | 0.548509 | 7.1:-52608350 | None:intergenic |
| ACGTGGTTGATGAAGTTCAA+AGG | 0.562344 | 7.1:-52608074 | None:intergenic |
| TCAACTTACAGGTGGATGGA+AGG | 0.567817 | 7.1:+52609104 | MS.gene050263:CDS |
| CCTTTCCAACGAGTTTCCAA+TGG | 0.571854 | 7.1:+52607989 | MS.gene050263:CDS |
| CTAAAATTTCAACTTACAGG+TGG | 0.580366 | 7.1:+52609096 | MS.gene050263:CDS |
| TAGAGTTGGAAAACTGCAGA+AGG | 0.585587 | 7.1:+52608120 | MS.gene050263:CDS |
| CTACTGCTTCCAATTTCACG+TGG | 0.605958 | 7.1:-52608091 | None:intergenic |
| TGGATGGAAGGCATTTGCAG+AGG | 0.673309 | 7.1:+52609116 | MS.gene050263:CDS |
| TGCACGCATGACAACAACAA+AGG | 0.693553 | 7.1:-52608354 | None:intergenic |
| CATACTGACAGAGAGTCCAA+AGG | 0.719746 | 7.1:+52608157 | MS.gene050263:CDS |
| TGTCTATTACCAAACATACG+TGG | 0.737084 | 7.1:-52608976 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTATATTCTAATTATCCT+TGG | - | chr7.1:52608535-52608554 | None:intergenic | 15.0% |
| !!! | AGTTATTTGATTATTTTGTT+AGG | + | chr7.1:52608578-52608597 | MS.gene050263:intron | 15.0% |
| !! | AAATGTGTATTAATCAAGAT+CGG | - | chr7.1:52608718-52608737 | None:intergenic | 20.0% |
| !! | TTAAGTTTCAATGACATATT+TGG | + | chr7.1:52608408-52608427 | MS.gene050263:intron | 20.0% |
| !!! | TGTTGTTATTTTGTTTATTC+TGG | + | chr7.1:52608615-52608634 | MS.gene050263:intron | 20.0% |
| !! | CATTTGTCTTTTCTTTTACT+TGG | + | chr7.1:52608944-52608963 | MS.gene050263:intron | 25.0% |
| !! | TATTTTGTTAGGTGATTGAT+AGG | + | chr7.1:52608589-52608608 | MS.gene050263:intron | 25.0% |
| !!! | TTTGATTTGGATAAGAAAAG+AGG | + | chr7.1:52609000-52609019 | MS.gene050263:CDS | 25.0% |
| AAGTAGCTTCTTCTTTCATT+TGG | - | chr7.1:52609357-52609376 | None:intergenic | 30.0% | |
| AAGTATTTGAATAACGCTTG+AGG | + | chr7.1:52608640-52608659 | MS.gene050263:intron | 30.0% | |
| AATCTAGTAAAGAGTAGAGA+AGG | - | chr7.1:52608456-52608475 | None:intergenic | 30.0% | |
| AGTATTTGAATAACGCTTGA+GGG | + | chr7.1:52608641-52608660 | MS.gene050263:intron | 30.0% | |
| ATTGTTCTTCATCTCAAAGT+AGG | + | chr7.1:52609244-52609263 | MS.gene050263:intron | 30.0% | |
| CAAGGAAATAAACGTTCAAA+TGG | + | chr7.1:52608013-52608032 | MS.gene050263:CDS | 30.0% | |
| CTAAAATTTCAACTTACAGG+TGG | + | chr7.1:52609096-52609115 | MS.gene050263:CDS | 30.0% | |
| CTCTACTCTTTACTAGATTA+CGG | + | chr7.1:52608457-52608476 | MS.gene050263:intron | 30.0% | |
| GAGGAAAATAACTTGAAAGT+TGG | + | chr7.1:52609135-52609154 | MS.gene050263:CDS | 30.0% | |
| GGACTAAAATTTCAACTTAC+AGG | + | chr7.1:52609093-52609112 | MS.gene050263:CDS | 30.0% | |
| TATCTAATCATGATGTCACT+TGG | - | chr7.1:52608826-52608845 | None:intergenic | 30.0% | |
| TGCAAAATTGGAACACAATA+AGG | - | chr7.1:52608261-52608280 | None:intergenic | 30.0% | |
| TGGTAATAGACACTTTGATT+TGG | + | chr7.1:52608987-52609006 | MS.gene050263:CDS | 30.0% | |
| ! | AAGAGGAGATATTCATTTTC+AGG | + | chr7.1:52609017-52609036 | MS.gene050263:CDS | 30.0% |
| ! | TATTGTGTTCCAATTTTGCA+GGG | + | chr7.1:52608261-52608280 | MS.gene050263:intron | 30.0% |
| ! | TTATTGTGTTCCAATTTTGC+AGG | + | chr7.1:52608260-52608279 | MS.gene050263:intron | 30.0% |
| !! | ACTTTGAGATGAAGAACAAT+TGG | - | chr7.1:52609244-52609263 | None:intergenic | 30.0% |
| !!! | CATTTTCAGGTGTTAAATGA+AGG | + | chr7.1:52609030-52609049 | MS.gene050263:CDS | 30.0% |
| AAGCCACAATCTTATACCTT+TGG | - | chr7.1:52608176-52608195 | None:intergenic | 35.0% | |
| AATTTCAACTTACAGGTGGA+TGG | + | chr7.1:52609100-52609119 | MS.gene050263:CDS | 35.0% | |
| ACGTTTATTTCCTTGACCAT+TGG | - | chr7.1:52608008-52608027 | None:intergenic | 35.0% | |
| AGGTGTTAAATGAAGGAGTT+TGG | + | chr7.1:52609037-52609056 | MS.gene050263:CDS | 35.0% | |
| ATCTTTCCATTGATACCAAC+TGG | + | chr7.1:52608758-52608777 | MS.gene050263:intron | 35.0% | |
| CAAAAACTATGTATGCACCA+AGG | + | chr7.1:52608515-52608534 | MS.gene050263:intron | 35.0% | |
| CAGAATATACCACGTATGTT+TGG | + | chr7.1:52608967-52608986 | MS.gene050263:intron | 35.0% | |
| CATTTGGAAAGATTCTGCAT+AGG | - | chr7.1:52609341-52609360 | None:intergenic | 35.0% | |
| GTAAAGAGTAGAGAAGGATT+TGG | - | chr7.1:52608450-52608469 | None:intergenic | 35.0% | |
| GTATTTGAATAACGCTTGAG+GGG | + | chr7.1:52608642-52608661 | MS.gene050263:intron | 35.0% | |
| TGTCTATTACCAAACATACG+TGG | - | chr7.1:52608979-52608998 | None:intergenic | 35.0% | |
| ! | AGTCCAAAGGTATAAGATTG+TGG | + | chr7.1:52608170-52608189 | MS.gene050263:intron | 35.0% |
| ! | CGTTGAACATCATTTTCTAC+TGG | + | chr7.1:52608382-52608401 | MS.gene050263:CDS | 35.0% |
| ! | TAATCATGATGTCACTTGGT+AGG | - | chr7.1:52608822-52608841 | None:intergenic | 35.0% |
| ! | TCTATCAAGAAGTGCAGTAA+AGG | + | chr7.1:52609072-52609091 | MS.gene050263:CDS | 35.0% |
| !!! | AGCAGTAGTTGTTTTAGAGT+TGG | + | chr7.1:52608106-52608125 | MS.gene050263:CDS | 35.0% |
| CACTTCTTTCCCTGCAAAAT+TGG | - | chr7.1:52608273-52608292 | None:intergenic | 40.0% | |
| CATTGGAAACTCGTTGGAAA+GGG | - | chr7.1:52607991-52608010 | None:intergenic | 40.0% | |
| CTTCATCAACCACGTGAAAT+TGG | + | chr7.1:52608082-52608101 | MS.gene050263:CDS | 40.0% | |
| TAGAGTTGGAAAACTGCAGA+AGG | + | chr7.1:52608120-52608139 | MS.gene050263:CDS | 40.0% | |
| TAGGATTGTCCATTTGAGCT+GGG | - | chr7.1:52608803-52608822 | None:intergenic | 40.0% | |
| TGGAAAGATTCTGCATAGGA+TGG | - | chr7.1:52609337-52609356 | None:intergenic | 40.0% | |
| !! | ACGTGGTTGATGAAGTTCAA+AGG | - | chr7.1:52608077-52608096 | None:intergenic | 40.0% |
| ATGAAGGAGTTTGGACTGCA+AGG | + | chr7.1:52609046-52609065 | MS.gene050263:CDS | 45.0% | |
| ATTTCACAACAGGGACCAGT+TGG | - | chr7.1:52608776-52608795 | None:intergenic | 45.0% | |
| CAACGAGTTTCCAATGGTCA+AGG | + | chr7.1:52607995-52608014 | MS.gene050263:CDS | 45.0% | |
| CATACTGACAGAGAGTCCAA+AGG | + | chr7.1:52608157-52608176 | MS.gene050263:CDS | 45.0% | |
| CCATTGGAAACTCGTTGGAA+AGG | - | chr7.1:52607992-52608011 | None:intergenic | 45.0% | |
| CCTTTCCAACGAGTTTCCAA+TGG | + | chr7.1:52607989-52608008 | MS.gene050263:CDS | 45.0% | |
| CGCATGACAACAACAAAGGA+TGG | - | chr7.1:52608353-52608372 | None:intergenic | 45.0% | |
| CTACTGCTTCCAATTTCACG+TGG | - | chr7.1:52608094-52608113 | None:intergenic | 45.0% | |
| CTGGGCACAATTTCACAACA+GGG | - | chr7.1:52608785-52608804 | None:intergenic | 45.0% | |
| CTTGACCATTGGAAACTCGT+TGG | - | chr7.1:52607997-52608016 | None:intergenic | 45.0% | |
| GAAATTGTGCCCAGCTCAAA+TGG | + | chr7.1:52608791-52608810 | MS.gene050263:intron | 45.0% | |
| GTAGGATTGTCCATTTGAGC+TGG | - | chr7.1:52608804-52608823 | None:intergenic | 45.0% | |
| TCAACTTACAGGTGGATGGA+AGG | + | chr7.1:52609104-52609123 | MS.gene050263:CDS | 45.0% | |
| TGAATAACGCTTGAGGGGAT+GGG | + | chr7.1:52608647-52608666 | MS.gene050263:intron | 45.0% | |
| TGCACGCATGACAACAACAA+AGG | - | chr7.1:52608357-52608376 | None:intergenic | 45.0% | |
| TTGAATAACGCTTGAGGGGA+TGG | + | chr7.1:52608646-52608665 | MS.gene050263:intron | 45.0% | |
| GCTGGGCACAATTTCACAAC+AGG | - | chr7.1:52608786-52608805 | None:intergenic | 50.0% | |
| ! | CAGGGACCAGTTGGTATCAA+TGG | - | chr7.1:52608767-52608786 | None:intergenic | 50.0% |
| !! | TGGATGGAAGGCATTTGCAG+AGG | + | chr7.1:52609116-52609135 | MS.gene050263:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 52607980 | 52609378 | 52607980 | ID=MS.gene050263 |
| chr7.1 | mRNA | 52607980 | 52609378 | 52607980 | ID=MS.gene050263.t1;Parent=MS.gene050263 |
| chr7.1 | exon | 52607980 | 52608178 | 52607980 | ID=MS.gene050263.t1.exon1;Parent=MS.gene050263.t1 |
| chr7.1 | CDS | 52607980 | 52608178 | 52607980 | ID=cds.MS.gene050263.t1;Parent=MS.gene050263.t1 |
| chr7.1 | exon | 52608282 | 52608403 | 52608282 | ID=MS.gene050263.t1.exon2;Parent=MS.gene050263.t1 |
| chr7.1 | CDS | 52608282 | 52608403 | 52608282 | ID=cds.MS.gene050263.t1;Parent=MS.gene050263.t1 |
| chr7.1 | exon | 52608970 | 52609261 | 52608970 | ID=MS.gene050263.t1.exon3;Parent=MS.gene050263.t1 |
| chr7.1 | CDS | 52608970 | 52609261 | 52608970 | ID=cds.MS.gene050263.t1;Parent=MS.gene050263.t1 |
| chr7.1 | exon | 52609347 | 52609378 | 52609347 | ID=MS.gene050263.t1.exon4;Parent=MS.gene050263.t1 |
| chr7.1 | CDS | 52609347 | 52609378 | 52609347 | ID=cds.MS.gene050263.t1;Parent=MS.gene050263.t1 |
| Gene Sequence |
| Protein sequence |
