Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050264.t1 | XP_003622663.1 | 83.7 | 196 | 31 | 1 | 2 | 197 | 121 | 315 | 7.50E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050264.t1 | Q9XIB5 | 29.1 | 127 | 84 | 2 | 46 | 172 | 101 | 221 | 1.9e-08 | 60.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050264.t1 | G7KVV4 | 83.7 | 196 | 31 | 1 | 2 | 197 | 121 | 315 | 5.4e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene050264.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene050264.t1 | MTR_7g050560 | 83.673 | 196 | 31 | 1 | 2 | 197 | 121 | 315 | 3.83e-122 | 348 |
| MS.gene050264.t1 | MTR_7g050590 | 71.429 | 182 | 48 | 4 | 6 | 185 | 114 | 293 | 5.72e-91 | 268 |
| MS.gene050264.t1 | MTR_7g050580 | 67.010 | 194 | 47 | 3 | 5 | 197 | 138 | 315 | 2.67e-82 | 247 |
| MS.gene050264.t1 | MTR_3g098790 | 65.193 | 181 | 55 | 3 | 6 | 185 | 139 | 312 | 3.53e-77 | 234 |
| MS.gene050264.t1 | MTR_7g050710 | 63.043 | 184 | 64 | 3 | 5 | 185 | 138 | 320 | 1.08e-73 | 225 |
| MS.gene050264.t1 | MTR_7g050720 | 52.514 | 179 | 75 | 6 | 6 | 174 | 139 | 317 | 6.23e-54 | 174 |
| MS.gene050264.t1 | MTR_1g108780 | 32.639 | 144 | 82 | 4 | 29 | 172 | 162 | 290 | 1.80e-17 | 80.1 |
| MS.gene050264.t1 | MTR_1g035460 | 39.640 | 111 | 55 | 4 | 63 | 172 | 188 | 287 | 2.38e-17 | 79.3 |
| MS.gene050264.t1 | MTR_8g103940 | 36.134 | 119 | 66 | 2 | 54 | 172 | 223 | 331 | 3.18e-14 | 70.5 |
| MS.gene050264.t1 | MTR_5g044670 | 33.621 | 116 | 72 | 2 | 60 | 175 | 196 | 306 | 2.47e-12 | 64.7 |
| MS.gene050264.t1 | MTR_4g068320 | 30.000 | 130 | 84 | 3 | 49 | 177 | 270 | 393 | 2.87e-12 | 64.7 |
| MS.gene050264.t1 | MTR_1g034240 | 28.378 | 148 | 95 | 4 | 49 | 191 | 274 | 415 | 1.55e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGTTAAATGAAGGAATT+TGG | 0.170862 | 7.1:+52599850 | MS.gene050264:CDS |
| TTCCATCAACCATGTGAAAT+TGG | 0.262872 | 7.1:+52599107 | MS.gene050264:CDS |
| CTAATCAGGATGACCCTTAC+AGG | 0.321649 | 7.1:+52599885 | MS.gene050264:CDS |
| ATCCTGATTAGGTACTTTGC+AGG | 0.384916 | 7.1:-52599873 | None:intergenic |
| TGGTAATAGACACTTTGATT+TGG | 0.391800 | 7.1:+52599803 | MS.gene050264:CDS |
| TTCCAATTTCACATGGTTGA+TGG | 0.398771 | 7.1:-52599109 | None:intergenic |
| CAGAATATACCACGTATGTT+TGG | 0.412403 | 7.1:+52599783 | MS.gene050264:intron |
| GTTTATTTGTTGTTAAATGA+AGG | 0.421453 | 7.1:+52599843 | MS.gene050264:CDS |
| GGCCTGCAAAGTACCTAATC+AGG | 0.423211 | 7.1:+52599871 | MS.gene050264:CDS |
| CTGTAAGGGTCATCCTGATT+AGG | 0.427523 | 7.1:-52599884 | None:intergenic |
| GATCAATTTGTGGTCCTGTA+AGG | 0.446598 | 7.1:-52599899 | None:intergenic |
| GCAGCTAAAATGGTCTCTAA+TGG | 0.456051 | 7.1:+52599053 | None:intergenic |
| GAAGAAGCTTCTCAAGGATA+AGG | 0.472251 | 7.1:+52600171 | MS.gene050264:CDS |
| ACTTTGAGATGCTGAACAAT+TGG | 0.487702 | 7.1:-52600054 | None:intergenic |
| TGAAGTTGAGAAATTGCAAA+AGG | 0.487851 | 7.1:+52599145 | MS.gene050264:CDS |
| GAGGACAATAACTTGAAAGT+TGG | 0.514989 | 7.1:+52599948 | MS.gene050264:CDS |
| TCTAAAGATGTGCACAATGA+AGG | 0.517293 | 7.1:-52600012 | None:intergenic |
| CGCAACTTACCAGACGAAAA+CGG | 0.520134 | 7.1:-52599470 | None:intergenic |
| CCAACTGTGAGATCAATTTG+TGG | 0.524959 | 7.1:-52599909 | None:intergenic |
| AATGAAGGCAAGTATAGTGC+TGG | 0.525559 | 7.1:-52599997 | None:intergenic |
| GAGAAGCTTCTTCTTTCACT+CGG | 0.532825 | 7.1:-52600161 | None:intergenic |
| ATCAATTTGTGGTCCTGTAA+GGG | 0.548089 | 7.1:-52599898 | None:intergenic |
| CGCATGGCAACAACAAAGGA+TGG | 0.550805 | 7.1:-52599429 | None:intergenic |
| CCACAAATTGATCTCACAGT+TGG | 0.558906 | 7.1:+52599909 | MS.gene050264:CDS |
| CTATTGCTTCCAATTTCACA+TGG | 0.560323 | 7.1:-52599116 | None:intergenic |
| TTTGATTTGGATAAGAAGAG+AGG | 0.576081 | 7.1:+52599816 | MS.gene050264:CDS |
| AAATTGATCTCACAGTTGGA+TGG | 0.578678 | 7.1:+52599913 | MS.gene050264:CDS |
| TGGTTGATGGAATTTAAAAG+AGG | 0.579281 | 7.1:-52599096 | None:intergenic |
| AGAAGCTTCTTCTTTCACTC+GGG | 0.589107 | 7.1:-52600160 | None:intergenic |
| GTAGCAACTTTATATCATGC+TGG | 0.590309 | 7.1:+52599167 | MS.gene050264:CDS |
| GTGAAAGAAGAAGCTTCTCA+AGG | 0.611713 | 7.1:+52600165 | MS.gene050264:CDS |
| TTCAACGTATGATGCACGCA+TGG | 0.638803 | 7.1:-52599445 | None:intergenic |
| TGCACGCATGGCAACAACAA+AGG | 0.639444 | 7.1:-52599433 | None:intergenic |
| TGGATGGAAGACATTTGCAG+AGG | 0.667798 | 7.1:+52599929 | MS.gene050264:CDS |
| TGTCTATTACCAAACATACG+TGG | 0.737084 | 7.1:-52599792 | None:intergenic |
| CATGCTGGCAGAGAGTCCAG+AGG | 0.751318 | 7.1:+52599182 | MS.gene050264:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATTGGAATATCAAA+TGG | + | chr7.1:52599568-52599587 | MS.gene050264:intron | 15.0% |
| !! | AAAAATTAGAAAGTTCTTGT+TGG | - | chr7.1:52599731-52599750 | None:intergenic | 20.0% |
| !!! | GTTTATTTGTTGTTAAATGA+AGG | + | chr7.1:52599843-52599862 | MS.gene050264:CDS | 20.0% |
| ! | ATATTGGAATATCAAATGGA+GGG | + | chr7.1:52599572-52599591 | MS.gene050264:intron | 25.0% |
| ! | ATCATCACATAGATGTTTAT+TGG | + | chr7.1:52599618-52599637 | MS.gene050264:intron | 25.0% |
| ! | TATATTGGAATATCAAATGG+AGG | + | chr7.1:52599571-52599590 | MS.gene050264:intron | 25.0% |
| ! | TATCTGATTGGATAATTGTT+AGG | - | chr7.1:52599599-52599618 | None:intergenic | 25.0% |
| ! | TGCAAAATTTGAACACAATA+AGG | - | chr7.1:52599337-52599356 | None:intergenic | 25.0% |
| ! | TGTTAACATAAATCTTTAGC+AGG | + | chr7.1:52599269-52599288 | MS.gene050264:intron | 25.0% |
| ! | TGTTGTTAAATGAAGGAATT+TGG | + | chr7.1:52599850-52599869 | MS.gene050264:CDS | 25.0% |
| !! | CATTTGTCTTTTCTTTTGAT+TGG | + | chr7.1:52599760-52599779 | MS.gene050264:intron | 25.0% |
| !! | TTATTGTGTTCAAATTTTGC+AGG | + | chr7.1:52599336-52599355 | MS.gene050264:intron | 25.0% |
| AAGGGGTGAGAAATATATAT+TGG | + | chr7.1:52599556-52599575 | MS.gene050264:intron | 30.0% | |
| GTAAAGACAAGAGAAAGAAT+TGG | - | chr7.1:52599530-52599549 | None:intergenic | 30.0% | |
| TCTATGTGATGATATCTGAT+TGG | - | chr7.1:52599611-52599630 | None:intergenic | 30.0% | |
| TCTTGTCTTTACCAGATTAA+GGG | + | chr7.1:52599538-52599557 | MS.gene050264:intron | 30.0% | |
| TGAAGTTGAGAAATTGCAAA+AGG | + | chr7.1:52599145-52599164 | MS.gene050264:CDS | 30.0% | |
| TGGTAATAGACACTTTGATT+TGG | + | chr7.1:52599803-52599822 | MS.gene050264:CDS | 30.0% | |
| !! | TGGTTGATGGAATTTAAAAG+AGG | - | chr7.1:52599099-52599118 | None:intergenic | 30.0% |
| !! | TTTGATTTGGATAAGAAGAG+AGG | + | chr7.1:52599816-52599835 | MS.gene050264:CDS | 30.0% |
| AAATTGATCTCACAGTTGGA+TGG | + | chr7.1:52599913-52599932 | MS.gene050264:CDS | 35.0% | |
| ATATTTCTCACCCCTTAATC+TGG | - | chr7.1:52599552-52599571 | None:intergenic | 35.0% | |
| ATCAATTTGTGGTCCTGTAA+GGG | - | chr7.1:52599901-52599920 | None:intergenic | 35.0% | |
| ATTGTTCAGCATCTCAAAGT+AGG | + | chr7.1:52600057-52600076 | MS.gene050264:intron | 35.0% | |
| CAGAATATACCACGTATGTT+TGG | + | chr7.1:52599783-52599802 | MS.gene050264:intron | 35.0% | |
| CTATTGCTTCCAATTTCACA+TGG | - | chr7.1:52599119-52599138 | None:intergenic | 35.0% | |
| CTCTTGTCTTTACCAGATTA+AGG | + | chr7.1:52599537-52599556 | MS.gene050264:intron | 35.0% | |
| CTTGTCTTTACCAGATTAAG+GGG | + | chr7.1:52599539-52599558 | MS.gene050264:intron | 35.0% | |
| GACACAAAATTGTATACCTC+TGG | - | chr7.1:52599201-52599220 | None:intergenic | 35.0% | |
| GAGGACAATAACTTGAAAGT+TGG | + | chr7.1:52599948-52599967 | MS.gene050264:CDS | 35.0% | |
| GTAAGTTGCGAAGAAACATT+TGG | + | chr7.1:52599483-52599502 | MS.gene050264:intron | 35.0% | |
| GTAGCAACTTTATATCATGC+TGG | + | chr7.1:52599167-52599186 | MS.gene050264:CDS | 35.0% | |
| TCTAAAGATGTGCACAATGA+AGG | - | chr7.1:52600015-52600034 | None:intergenic | 35.0% | |
| TGTCTATTACCAAACATACG+TGG | - | chr7.1:52599795-52599814 | None:intergenic | 35.0% | |
| TTCCAATTTCACATGGTTGA+TGG | - | chr7.1:52599112-52599131 | None:intergenic | 35.0% | |
| TTCCATCAACCATGTGAAAT+TGG | + | chr7.1:52599107-52599126 | MS.gene050264:CDS | 35.0% | |
| ! | GTTGTAATTTTCTCAGCAGT+AGG | - | chr7.1:52599375-52599394 | None:intergenic | 35.0% |
| !! | ACTTTGAGATGCTGAACAAT+TGG | - | chr7.1:52600057-52600076 | None:intergenic | 35.0% |
| AATGAAGGCAAGTATAGTGC+TGG | - | chr7.1:52600000-52600019 | None:intergenic | 40.0% | |
| AGAAGCTTCTTCTTTCACTC+GGG | - | chr7.1:52600163-52600182 | None:intergenic | 40.0% | |
| ATCCTGATTAGGTACTTTGC+AGG | - | chr7.1:52599876-52599895 | None:intergenic | 40.0% | |
| CCAACTGTGAGATCAATTTG+TGG | - | chr7.1:52599912-52599931 | None:intergenic | 40.0% | |
| CCACAAATTGATCTCACAGT+TGG | + | chr7.1:52599909-52599928 | MS.gene050264:CDS | 40.0% | |
| GAGAAGCTTCTTCTTTCACT+CGG | - | chr7.1:52600164-52600183 | None:intergenic | 40.0% | |
| GATCAATTTGTGGTCCTGTA+AGG | - | chr7.1:52599902-52599921 | None:intergenic | 40.0% | |
| GTGAAAGAAGAAGCTTCTCA+AGG | + | chr7.1:52600165-52600184 | MS.gene050264:CDS | 40.0% | |
| CGCAACTTACCAGACGAAAA+CGG | - | chr7.1:52599473-52599492 | None:intergenic | 45.0% | |
| CTAATCAGGATGACCCTTAC+AGG | + | chr7.1:52599885-52599904 | MS.gene050264:CDS | 45.0% | |
| CTGTAAGGGTCATCCTGATT+AGG | - | chr7.1:52599887-52599906 | None:intergenic | 45.0% | |
| TGGATGGAAGACATTTGCAG+AGG | + | chr7.1:52599929-52599948 | MS.gene050264:CDS | 45.0% | |
| TTCAACGTATGATGCACGCA+TGG | - | chr7.1:52599448-52599467 | None:intergenic | 45.0% | |
| CGCATGGCAACAACAAAGGA+TGG | - | chr7.1:52599432-52599451 | None:intergenic | 50.0% | |
| TGCACGCATGGCAACAACAA+AGG | - | chr7.1:52599436-52599455 | None:intergenic | 50.0% | |
| ! | CGTTGAACACCGTTTTCGTC+TGG | + | chr7.1:52599461-52599480 | MS.gene050264:CDS | 50.0% |
| ! | GGCCTGCAAAGTACCTAATC+AGG | + | chr7.1:52599871-52599890 | MS.gene050264:CDS | 50.0% |
| CATGCTGGCAGAGAGTCCAG+AGG | + | chr7.1:52599182-52599201 | MS.gene050264:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 52599062 | 52600191 | 52599062 | ID=MS.gene050264 |
| chr7.1 | mRNA | 52599062 | 52600191 | 52599062 | ID=MS.gene050264.t1;Parent=MS.gene050264 |
| chr7.1 | exon | 52599062 | 52599203 | 52599062 | ID=MS.gene050264.t1.exon1;Parent=MS.gene050264.t1 |
| chr7.1 | CDS | 52599062 | 52599203 | 52599062 | ID=cds.MS.gene050264.t1;Parent=MS.gene050264.t1 |
| chr7.1 | exon | 52599358 | 52599482 | 52599358 | ID=MS.gene050264.t1.exon2;Parent=MS.gene050264.t1 |
| chr7.1 | CDS | 52599358 | 52599482 | 52599358 | ID=cds.MS.gene050264.t1;Parent=MS.gene050264.t1 |
| chr7.1 | exon | 52599786 | 52600074 | 52599786 | ID=MS.gene050264.t1.exon3;Parent=MS.gene050264.t1 |
| chr7.1 | CDS | 52599786 | 52600074 | 52599786 | ID=cds.MS.gene050264.t1;Parent=MS.gene050264.t1 |
| chr7.1 | exon | 52600154 | 52600191 | 52600154 | ID=MS.gene050264.t1.exon4;Parent=MS.gene050264.t1 |
| chr7.1 | CDS | 52600154 | 52600191 | 52600154 | ID=cds.MS.gene050264.t1;Parent=MS.gene050264.t1 |
| Gene Sequence |
| Protein sequence |
