AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05042

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05042.t1 MTR_3g005420 83.005 406 65 3 1 404 20 423 0.0 689
MS.gene05042.t1 MTR_3g005420 52.874 261 101 1 7 245 273 533 2.39e-82 262
MS.gene05042.t1 MTR_3g005420 75.862 406 59 4 1 404 1 369 0.0 612
MS.gene05042.t1 MTR_3g005420 48.117 239 111 1 7 245 254 479 1.50e-61 206
MS.gene05042.t1 MTR_3g005460 68.059 407 117 4 1 404 1 397 0.0 539
MS.gene05042.t1 MTR_3g005460 50.382 262 100 4 18 249 257 518 1.24e-76 247
MS.gene05042.t1 MTR_3g005440 72.802 364 70 2 37 398 34 370 0.0 533
MS.gene05042.t1 MTR_3g005440 51.691 207 71 2 18 202 237 436 5.59e-61 204
MS.gene05042.t1 MTR_3g005495 68.258 356 96 3 38 393 28 366 1.89e-167 478
MS.gene05042.t1 MTR_3g005495 49.167 240 109 6 18 247 238 474 3.39e-61 205
MS.gene05042.t1 MTR_3g005495 60.331 121 48 0 285 405 28 148 7.04e-42 154
MS.gene05042.t1 MTR_5g070440 57.678 267 89 2 1 245 1 265 3.90e-98 294
MS.gene05042.t1 MTR_5g070440 71.429 140 40 0 265 404 16 155 1.22e-64 208
MS.gene05042.t1 MTR_3g005490 59.690 258 89 3 1 245 1 256 1.59e-97 292
MS.gene05042.t1 MTR_3g005490 69.343 137 42 0 265 401 16 152 1.40e-63 205
MS.gene05042.t1 MTR_3g005480 58.301 259 92 3 1 245 1 257 3.96e-93 281
MS.gene05042.t1 MTR_3g005480 67.153 137 45 0 265 401 16 152 9.28e-61 197
MS.gene05042.t1 MTR_1g034240 47.475 99 52 0 270 368 7 105 9.32e-27 111
MS.gene05042.t1 MTR_1g034240 43.434 99 56 0 23 121 7 105 2.31e-23 101
MS.gene05042.t1 MTR_1g034240 42.202 109 54 4 143 245 284 389 9.56e-12 66.6
MS.gene05042.t1 MTR_4g068320 42.157 102 59 0 267 368 3 104 1.15e-22 99.8
MS.gene05042.t1 MTR_4g068320 39.216 102 62 0 20 121 3 104 6.02e-19 88.6
MS.gene05042.t1 MTR_4g068320 42.727 110 54 4 143 246 280 386 1.83e-12 68.9
MS.gene05042.t1 MTR_1g015350 36.752 234 84 9 1 231 1 173 7.90e-22 92.0
MS.gene05042.t1 MTR_1g015350 49.367 79 27 1 313 391 58 123 2.25e-16 77.0
MS.gene05042.t1 MTR_1g034210 45.556 90 49 0 270 359 7 96 1.82e-21 94.7
MS.gene05042.t1 MTR_1g034210 38.835 103 63 0 23 125 7 109 4.25e-19 87.8
MS.gene05042.t1 MTR_1g034210 45.556 90 49 0 270 359 7 96 3.23e-21 94.7
MS.gene05042.t1 MTR_1g034210 38.095 105 65 0 23 127 7 111 7.77e-19 87.8
MS.gene05042.t1 MTR_3g098790 35.294 136 86 1 269 404 12 145 4.60e-21 93.6
MS.gene05042.t1 MTR_3g098790 24.468 282 150 5 16 235 6 286 6.30e-19 87.4
MS.gene05042.t1 MTR_7g050580 25.267 281 158 6 16 244 6 286 2.09e-19 88.6
MS.gene05042.t1 MTR_7g050580 34.400 125 81 1 263 387 7 130 5.06e-19 87.4
MS.gene05042.t1 MTR_7g050560 32.558 129 83 1 254 382 1 125 3.42e-19 88.2
MS.gene05042.t1 MTR_7g050560 31.250 112 77 0 22 133 12 123 1.84e-16 80.1
MS.gene05042.t1 MTR_3g061370 26.224 286 139 6 19 244 5 278 3.80e-19 87.4
MS.gene05042.t1 MTR_3g061370 32.847 137 90 1 267 403 6 140 4.14e-15 75.5
MS.gene05042.t1 MTR_7g050710 24.315 292 152 6 22 244 12 303 4.46e-19 87.8
MS.gene05042.t1 MTR_7g050710 32.787 122 80 1 263 382 4 125 6.18e-17 81.6
MS.gene05042.t1 MTR_5g044670 35.849 106 68 0 263 368 9 114 9.71e-19 87.0
MS.gene05042.t1 MTR_5g044670 24.746 295 150 7 20 247 13 302 7.14e-18 84.3
MS.gene05042.t1 MTR_3g061310 26.277 274 147 5 23 244 12 282 1.07e-18 85.9
MS.gene05042.t1 MTR_3g061310 40.404 99 57 1 270 368 12 108 3.93e-16 78.6
MS.gene05042.t1 MTR_3g061350 27.106 273 145 7 23 244 12 281 2.35e-18 85.1
MS.gene05042.t1 MTR_3g061350 30.597 134 91 1 270 403 12 143 3.22e-14 73.2
MS.gene05042.t1 MTR_7g050720 36.607 112 71 0 269 380 9 120 4.89e-18 84.7
MS.gene05042.t1 MTR_7g050720 35.644 101 65 0 22 122 9 109 3.78e-16 79.3
MS.gene05042.t1 MTR_8g103940 32.432 111 75 0 270 380 39 149 2.68e-17 84.0
MS.gene05042.t1 MTR_8g103940 23.132 281 149 5 23 236 39 319 1.61e-15 78.6
MS.gene05042.t1 MTR_3g061320 27.839 273 140 9 22 244 11 276 2.73e-17 82.0
MS.gene05042.t1 MTR_3g061320 41.000 100 57 1 269 368 11 108 3.77e-16 78.6
MS.gene05042.t1 MTR_3g061290 29.082 196 116 6 22 201 11 199 1.48e-16 78.2
MS.gene05042.t1 MTR_3g061290 34.615 130 72 2 269 398 11 127 3.07e-16 77.4
MS.gene05042.t1 MTR_3g061340 26.545 275 140 9 23 247 12 274 5.78e-16 77.8
MS.gene05042.t1 MTR_3g061340 41.000 100 56 2 270 368 12 109 4.96e-13 69.3
MS.gene05042.t1 MTR_7g050590 37.374 99 60 1 286 382 2 100 1.03e-15 77.4
MS.gene05042.t1 MTR_7g050590 33.333 111 69 2 39 144 2 112 1.08e-14 74.7
MS.gene05042.t1 MTR_3g061330 40.404 99 57 1 270 368 12 108 7.46e-15 74.3
MS.gene05042.t1 MTR_3g061330 38.384 99 59 1 23 121 12 108 5.18e-14 72.0
MS.gene05042.t1 MTR_6g043890 33.813 139 89 2 254 391 1 137 8.26e-15 75.5
MS.gene05042.t1 MTR_3g061220 38.384 99 46 2 23 121 12 95 1.34e-14 70.1
MS.gene05042.t1 MTR_3g061220 37.374 99 47 2 270 368 12 95 9.36e-13 65.1
MS.gene05042.t1 MTR_1g035460 40.000 85 51 0 286 370 2 86 8.58e-14 72.8
MS.gene05042.t1 MTR_1g035460 34.118 85 56 0 39 123 2 86 8.48e-12 66.6
MS.gene05042.t1 MTR_1g108780 33.333 96 64 0 285 380 12 107 9.52e-14 73.2
MS.gene05042.t1 MTR_1g108780 30.208 96 67 0 38 133 12 107 5.34e-11 64.3
MS.gene05042.t1 MTR_6g061070 32.374 139 82 3 244 382 1 127 1.42e-13 72.4
MS.gene05042.t1 MTR_4g119990 32.886 149 97 3 258 404 2 149 1.66e-13 72.0
MS.gene05042.t1 MTR_1g021270 34.021 97 63 1 269 365 15 110 3.02e-13 71.6
MS.gene05042.t1 MTR_1g035440 30.882 136 91 2 254 388 1 134 6.59e-13 68.6
MS.gene05042.t1 MTR_1g035440 31.579 133 79 3 22 144 14 144 3.87e-11 63.5
MS.gene05042.t1 MTR_1g035480 28.467 137 95 2 254 390 1 134 1.32e-12 66.2
MS.gene05042.t1 MTR_7g050640 33.654 104 69 0 269 372 12 115 2.07e-12 63.9
MS.gene05042.t1 MTR_7g050640 29.730 111 78 0 16 126 6 116 1.43e-11 61.6
MS.gene05042.t1 MTR_0152s0100 36.538 104 65 1 141 244 2 104 2.77e-12 63.5
MS.gene05042.t1 MTR_0152s0100 36.538 104 65 1 141 244 2 104 2.77e-12 63.5
MS.gene05042.t1 MTR_1g021400 33.684 95 63 0 269 363 29 123 3.04e-12 67.8
MS.gene05042.t1 MTR_1g021320 33.065 124 75 3 244 360 150 272 3.69e-12 68.6
MS.gene05042.t1 MTR_1g035500 28.777 139 87 3 252 390 16 142 4.54e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05042.t1 AT3G18990 46.465 99 53 0 270 368 6 104 8.03e-25 104
MS.gene05042.t1 AT3G18990 39.394 99 60 0 23 121 6 104 1.54e-19 89.4
MS.gene05042.t1 AT3G18990 41.818 110 56 3 143 247 229 335 2.06e-12 68.2
MS.gene05042.t1 AT3G18990 46.465 99 53 0 270 368 6 104 1.43e-24 104
MS.gene05042.t1 AT3G18990 39.394 99 60 0 23 121 6 104 2.60e-19 89.4
MS.gene05042.t1 AT3G18990 42.202 109 55 3 143 246 229 334 2.14e-12 68.6
MS.gene05042.t1 AT3G18960 33.333 165 104 2 243 405 1 161 6.41e-20 87.4
MS.gene05042.t1 AT3G18960 33.945 109 72 0 15 123 22 130 5.32e-17 79.3
MS.gene05042.t1 AT3G18960 33.537 164 103 2 243 404 1 160 1.04e-19 87.4
MS.gene05042.t1 AT3G18960 33.945 109 72 0 15 123 22 130 1.14e-16 78.6
MS.gene05042.t1 AT1G49475 41.441 111 64 1 268 378 32 141 2.19e-19 85.9
MS.gene05042.t1 AT1G49475 34.286 140 83 2 1 132 4 142 8.14e-18 81.6
MS.gene05042.t1 AT4G01580 40.777 103 61 0 268 370 28 130 3.55e-19 85.1
MS.gene05042.t1 AT4G01580 34.862 109 71 0 15 123 22 130 1.87e-17 80.5
MS.gene05042.t1 AT3G18960 33.607 122 77 1 283 404 6 123 1.08e-14 72.0
MS.gene05042.t1 AT3G18960 34.091 88 58 0 36 123 6 93 2.21e-12 65.5
MS.gene05042.t1 AT1G26680 22.946 353 231 13 22 350 14 349 4.21e-14 74.7
MS.gene05042.t1 AT1G26680 22.946 353 231 13 22 350 14 349 4.44e-14 74.7
MS.gene05042.t1 AT1G26680 22.946 353 231 13 22 350 14 349 5.31e-14 74.3
MS.gene05042.t1 AT1G26680 22.946 353 231 13 22 350 14 349 6.13e-14 74.3
MS.gene05042.t1 AT2G24645 23.410 393 251 19 20 391 3 366 2.79e-13 71.6
MS.gene05042.t1 AT1G49480 44.144 111 52 5 143 247 114 220 3.21e-13 69.3
MS.gene05042.t1 AT1G49480 44.144 111 52 5 143 247 114 220 3.21e-13 69.3
MS.gene05042.t1 AT1G49480 44.144 111 52 5 143 247 114 220 3.21e-13 69.3
MS.gene05042.t1 AT2G24645 23.410 393 251 19 20 391 3 366 5.14e-13 70.9
MS.gene05042.t1 AT1G26680 23.446 354 229 14 22 350 14 350 5.96e-13 71.2
MS.gene05042.t1 AT1G26680 23.442 337 218 13 39 350 16 337 4.59e-12 68.6
MS.gene05042.t1 AT2G24645 23.000 400 252 19 20 391 3 374 7.35e-12 67.4
MS.gene05042.t1 AT2G24645 21.779 326 226 11 78 391 44 352 1.22e-11 66.6

Find 69 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGCTTTCCTTGAGAATTT+TGG 0.150373 3.4:+65982785 None:intergenic
GAATACTATTCTATTTATTA+TGG 0.217855 3.4:-65983174 MS.gene05042:CDS
TATTGTGTATTGCATGAAAA+AGG 0.238853 3.4:-65982754 MS.gene05042:CDS
AATTACTATTCGTTAGGATA+TGG 0.265897 3.4:-65981719 MS.gene05042:CDS
GATGAGTTCATAACAAGATT+TGG 0.268341 3.4:-65981857 MS.gene05042:CDS
TACTATCTCTTTGTCATGAA+TGG 0.314672 3.4:+65982385 None:intergenic
AAGGGAAAACAATCTATTAA+AGG 0.316256 3.4:-65982689 MS.gene05042:CDS
GCTCTTCTGGAAGCAGAGTT+AGG 0.343489 3.4:+65983024 None:intergenic
CTACTTCTATGAAAAGTTCC+AGG 0.344709 3.4:-65981568 MS.gene05042:CDS
ATGCGAATAACGGGTGGATT+CGG 0.369669 3.4:-65982721 MS.gene05042:CDS
CGATGGTCGTGAATGGGAAA+TGG 0.374916 3.4:-65983253 MS.gene05042:CDS
ATGAATGGGCGAGGGAAGTA+TGG 0.376143 3.4:+65982400 None:intergenic
ATGAGTTCATAACAAGATTT+GGG 0.379410 3.4:-65981856 MS.gene05042:CDS
GTTATCACTGCAGAGAATTC+CGG 0.381761 3.4:-65981879 MS.gene05042:intron
TTTCTGTCTTTCAAATATGA+AGG 0.422326 3.4:-65981692 MS.gene05042:CDS
TTTGCAAATTACTATTCGTT+AGG 0.423437 3.4:-65981725 MS.gene05042:CDS
TTTGAATCTTTCTCCGGCTC+CGG 0.427229 3.4:+65984039 None:intergenic
CAGTTCCCGATGGTCGTGAT+TGG 0.434376 3.4:-65981805 MS.gene05042:CDS
TCTTTCTCCGGCTCCGGTGA+CGG 0.437396 3.4:+65984045 None:intergenic
AGGGAAAACAATCTATTAAA+GGG 0.437546 3.4:-65982688 MS.gene05042:CDS
TATTTAGATTTCAAGTATGA+AGG 0.439885 3.4:-65983147 MS.gene05042:CDS
CAATGACAGAAAAGGAAGTA+AGG 0.446214 3.4:-65982445 MS.gene05042:CDS
TATTGCAATGCAGCCTCCAC+GGG 0.446625 3.4:-65982483 MS.gene05042:intron
ACTATCTCTTTGTCATGAAT+GGG 0.448674 3.4:+65982386 None:intergenic
AAATCACAAATGCGAATAAC+GGG 0.449750 3.4:-65982730 MS.gene05042:CDS
CTGTTCCCGATGGTCGTGAA+TGG 0.460301 3.4:-65983260 MS.gene05042:CDS
AAAATCACAAATGCGAATAA+CGG 0.478575 3.4:-65982731 MS.gene05042:CDS
AATCTTGTTATGAACTCATC+CGG 0.487752 3.4:+65981860 None:intergenic
TTCTGTCTTTCAAATATGAA+GGG 0.490414 3.4:-65981691 MS.gene05042:CDS
CTCTTTGTCATGAATGGGCG+AGG 0.492559 3.4:+65982391 None:intergenic
CATTTCCCATTCACGACCAT+CGG 0.495250 3.4:+65983254 None:intergenic
GATGGTCGTGAATGGGAAAT+GGG 0.497506 3.4:-65983252 MS.gene05042:CDS
ATTTCCCATTCACGACCATC+GGG 0.501758 3.4:+65983255 None:intergenic
TCCGACACCGTCACCGGAGC+CGG 0.503889 3.4:-65984052 MS.gene05042:CDS
GCTGTAACAAGTAGTACAAC+AGG 0.510742 3.4:+65982631 None:intergenic
TCCGGCTCCGGTGACGGTGT+CGG 0.512245 3.4:+65984051 None:intergenic
TGTTCCCGATGGTCGTGAAT+GGG 0.514313 3.4:-65983259 MS.gene05042:CDS
ACTGTTGCCAATTGTTGCTA+AGG 0.515477 3.4:+65983202 None:intergenic
TGCCCAGTCAATGACAGAAA+AGG 0.524540 3.4:-65982453 MS.gene05042:CDS
ACTGCCACTTGTTTCAACCC+TGG 0.525622 3.4:+65981550 None:intergenic
GTGTCGGAACACATCTTCTC+CGG 0.535373 3.4:+65984067 None:intergenic
ATTTAGATTTCAAGTATGAA+GGG 0.536271 3.4:-65983146 MS.gene05042:CDS
GTGGATTCGGTAAATTTGCA+AGG 0.541422 3.4:-65982708 MS.gene05042:CDS
GTTGAAACAAGTGGCAGTCA+AGG 0.541942 3.4:-65981545 MS.gene05042:CDS
TTATTGCAATGCAGCCTCCA+CGG 0.552353 3.4:-65982484 MS.gene05042:intron
ATGTGTTCCGACACCGTCAC+CGG 0.555592 3.4:-65984058 MS.gene05042:CDS
TGAAAAGTATCTGACACTAA+AGG 0.562551 3.4:-65982821 MS.gene05042:CDS
TATTGTATTTGATGTAACTT+CGG 0.567373 3.4:-65981651 MS.gene05042:CDS
AATGACAGAAAAGGAAGTAA+GGG 0.571733 3.4:-65982444 MS.gene05042:CDS
TACTTCTATGAAAAGTTCCA+GGG 0.583386 3.4:-65981567 MS.gene05042:CDS
TTCAAGCTCCAAAATTCTCA+AGG 0.596256 3.4:-65982793 MS.gene05042:CDS
ATGTGTTTGAGCTGATCAAG+AGG 0.597303 3.4:-65982657 MS.gene05042:CDS
AAAATTCTCAAGGAAAGCAG+TGG 0.599450 3.4:-65982783 MS.gene05042:CDS
GCTAAGATTACTGTTCCCGA+TGG 0.603036 3.4:-65983270 MS.gene05042:CDS
ACAAAACAATGAAGACTCGC+CGG 0.610644 3.4:-65984086 None:intergenic
TTGAAACAAGTGGCAGTCAA+GGG 0.614317 3.4:-65981544 MS.gene05042:CDS
TACTGCATAAAAGCCCCTGA+CGG 0.614950 3.4:+65982948 None:intergenic
ACTTGTTACAGCGATTTGCA+CGG 0.615517 3.4:-65982620 MS.gene05042:intron
TGGATTCGGTAAATTTGCAA+GGG 0.623971 3.4:-65982707 MS.gene05042:CDS
TCACAAATGCGAATAACGGG+TGG 0.632518 3.4:-65982727 MS.gene05042:CDS
AGTTCCAGGGTTGAAACAAG+TGG 0.632824 3.4:-65981554 MS.gene05042:CDS
TGGGCAGACATTCCCCGTGG+AGG 0.633574 3.4:+65982470 None:intergenic
AAATTCTCAAGGAAAGCAGT+GGG 0.637805 3.4:-65982782 MS.gene05042:CDS
TTTCGATCAAAATCCGTCAG+GGG 0.644334 3.4:-65982961 MS.gene05042:CDS
TCTTTGTCATGAATGGGCGA+GGG 0.647829 3.4:+65982392 None:intergenic
GCTACAATTACAGTTCCCGA+TGG 0.653468 3.4:-65981815 MS.gene05042:CDS
GAAATGGGACTGAAGAAATG+TGG 0.715375 3.4:-65983237 MS.gene05042:CDS
GACTGGGCAGACATTCCCCG+TGG 0.720587 3.4:+65982467 None:intergenic
ATTGCAATGCAGCCTCCACG+GGG 0.736037 3.4:-65982482 MS.gene05042:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTATTCTTCTTATTA+TGG + chr3.4:65983383-65983402 None:intergenic 10.0%
!! AAATATTCAAAATTTGTATG+AGG - chr3.4:65981684-65981703 MS.gene05042:CDS 15.0%
!! GAATACTATTCTATTTATTA+TGG - chr3.4:65982424-65982443 MS.gene05042:CDS 15.0%
!!! TTATTTCTTATTATTGTTGT+TGG - chr3.4:65981760-65981779 MS.gene05042:CDS 15.0%
!! AATAGTGAGAAAAACATAAA+GGG - chr3.4:65982550-65982569 MS.gene05042:intron 20.0%
!! ACGAGTTTATAAAAAGATAT+GGG - chr3.4:65982287-65982306 MS.gene05042:intron 20.0%
!! TAATAGTGAGAAAAACATAA+AGG - chr3.4:65982549-65982568 MS.gene05042:intron 20.0%
!! TACCTTTAAACATCAAATTA+GGG - chr3.4:65983637-65983656 MS.gene05042:intron 20.0%
!! TACGGTATGATTTATATTTA+TGG - chr3.4:65982700-65982719 MS.gene05042:CDS 20.0%
!! TATTGTATTTGATGTAACTT+CGG - chr3.4:65983947-65983966 MS.gene05042:intron 20.0%
!! TCATCTGTTATACTATAATA+CGG + chr3.4:65983696-65983715 None:intergenic 20.0%
!! TTATAGATAAAGCTAGAAAT+TGG - chr3.4:65981898-65981917 MS.gene05042:intron 20.0%
!!! ATTTAGATTTCAAGTATGAA+GGG - chr3.4:65982452-65982471 MS.gene05042:CDS 20.0%
!!! ATTTTGAATCTTTGATTGAT+TGG - chr3.4:65981819-65981838 MS.gene05042:CDS 20.0%
!!! TATTTAGATTTCAAGTATGA+AGG - chr3.4:65982451-65982470 MS.gene05042:CDS 20.0%
! AAAATCACAAATGCGAATAA+CGG - chr3.4:65982867-65982886 MS.gene05042:intron 25.0%
! AAGGGAAAACAATCTATTAA+AGG - chr3.4:65982909-65982928 MS.gene05042:intron 25.0%
! AATTACTATTCGTTAGGATA+TGG - chr3.4:65983879-65983898 MS.gene05042:intron 25.0%
! AGCATAAAAATCATGTTTGA+TGG - chr3.4:65982001-65982020 MS.gene05042:intron 25.0%
! AGGGAAAACAATCTATTAAA+GGG - chr3.4:65982910-65982929 MS.gene05042:intron 25.0%
! ATAAAAATCATGTTTGATGG+CGG - chr3.4:65982004-65982023 MS.gene05042:intron 25.0%
! ATCAATCAAACTTCAAAAGA+GGG + chr3.4:65982219-65982238 None:intergenic 25.0%
! ATGAGTTCATAACAAGATTT+GGG - chr3.4:65983742-65983761 MS.gene05042:intron 25.0%
! CTACCTTTAAACATCAAATT+AGG - chr3.4:65983636-65983655 MS.gene05042:intron 25.0%
! CTCAAAAATCTAAGTACTAA+TGG - chr3.4:65983987-65984006 MS.gene05042:intron 25.0%
! GACGAGTTTATAAAAAGATA+TGG - chr3.4:65982286-65982305 MS.gene05042:intron 25.0%
! TATTGTGTATTGCATGAAAA+AGG - chr3.4:65982844-65982863 MS.gene05042:CDS 25.0%
! TTCCCTAATTTGATGTTTAA+AGG + chr3.4:65983642-65983661 None:intergenic 25.0%
! TTCTGTCTTTCAAATATGAA+GGG - chr3.4:65983907-65983926 MS.gene05042:intron 25.0%
! TTTCTGTCTTTCAAATATGA+AGG - chr3.4:65983906-65983925 MS.gene05042:intron 25.0%
! TTTGCAAATTACTATTCGTT+AGG - chr3.4:65983873-65983892 MS.gene05042:intron 25.0%
!! ACATTTTCTTTTACAACGAA+TGG - chr3.4:65983844-65983863 MS.gene05042:intron 25.0%
!! AGCAACAATATTTGATTTAC+AGG + chr3.4:65981736-65981755 None:intergenic 25.0%
!! GTAAAAAAGCATATGTGATT+TGG - chr3.4:65983287-65983306 MS.gene05042:CDS 25.0%
!! TATCTTTTTATAAACTCGTC+TGG + chr3.4:65982286-65982305 None:intergenic 25.0%
!! TGTTTAAAGGTAGTTTTTCA+AGG + chr3.4:65983629-65983648 None:intergenic 25.0%
!!! AAATGTTTTGAATCTTTCTC+CGG + chr3.4:65981568-65981587 None:intergenic 25.0%
!!! ATCGAAAATAAGGGTTTTTT+GGG + chr3.4:65982624-65982643 None:intergenic 25.0%
!!! CTTTCTGGTTTTTAAAGTTA+CGG - chr3.4:65982682-65982701 MS.gene05042:CDS 25.0%
!!! GTTTAAAGGTAGTTTTTCAA+GGG + chr3.4:65983628-65983647 None:intergenic 25.0%
!!! TTAGTACTTAGATTTTTGAG+TGG + chr3.4:65983987-65984006 None:intergenic 25.0%
!!! TTAGTACTTGAAGTTTTGAA+TGG + chr3.4:65982532-65982551 None:intergenic 25.0%
!!! TTGTTGGTTGTTTTTATGTT+TGG - chr3.4:65981776-65981795 MS.gene05042:CDS 25.0%
AAATCACAAATGCGAATAAC+GGG - chr3.4:65982868-65982887 MS.gene05042:intron 30.0%
AATGACAGAAAAGGAAGTAA+GGG - chr3.4:65983154-65983173 MS.gene05042:CDS 30.0%
ACTATCTCTTTGTCATGAAT+GGG + chr3.4:65983215-65983234 None:intergenic 30.0%
AGCATGAAAATATTCAGCTT+TGG - chr3.4:65983421-65983440 MS.gene05042:intron 30.0%
ATTTGTCTATGTCTCTGATT+TGG - chr3.4:65983444-65983463 MS.gene05042:intron 30.0%
CGAAATTGATTGAAACGTTT+AGG + chr3.4:65982087-65982106 None:intergenic 30.0%
CTAACTTGAAAGGTAGTATT+TGG + chr3.4:65983522-65983541 None:intergenic 30.0%
CTAGACACATACATTTGATT+TGG + chr3.4:65983593-65983612 None:intergenic 30.0%
CTATAATACGGAACTTCAAA+AGG + chr3.4:65983684-65983703 None:intergenic 30.0%
CTCATCAGTTATACTACTAT+AGG + chr3.4:65982242-65982261 None:intergenic 30.0%
GATCAATCAAACTTCAAAAG+AGG + chr3.4:65982220-65982239 None:intergenic 30.0%
GATGAGTTCATAACAAGATT+TGG - chr3.4:65983741-65983760 MS.gene05042:intron 30.0%
TACTATCTCTTTGTCATGAA+TGG + chr3.4:65983216-65983235 None:intergenic 30.0%
TACTTCTATGAAAAGTTCCA+GGG - chr3.4:65984031-65984050 MS.gene05042:CDS 30.0%
TCAATCAAACTTCAAAAGAG+GGG + chr3.4:65982218-65982237 None:intergenic 30.0%
TTATGCATTAAGTTCACCAA+CGG + chr3.4:65983076-65983095 None:intergenic 30.0%
! AATCTTGTTATGAACTCATC+CGG + chr3.4:65983741-65983760 None:intergenic 30.0%
! AGATTCAGTTCTAACTTGAA+AGG + chr3.4:65983532-65983551 None:intergenic 30.0%
! ATGTTTGGAATCATTGTTGT+GGG + chr3.4:65982131-65982150 None:intergenic 30.0%
! ATGTTTTCCTTAGCAACAAT+TGG - chr3.4:65982389-65982408 MS.gene05042:CDS 30.0%
! CAATATTTGATTTACAGGAG+AGG + chr3.4:65981731-65981750 None:intergenic 30.0%
! TACTTTTCAGCAAAACTAGA+AGG + chr3.4:65982766-65982785 None:intergenic 30.0%
! TTCAAGTTAGAACTGAATCT+AGG - chr3.4:65983532-65983551 MS.gene05042:intron 30.0%
!! ACGGATTTTGATCGAAAATA+AGG + chr3.4:65982634-65982653 None:intergenic 30.0%
!! ACTATTTCTTTTGTGTGATC+TGG + chr3.4:65981613-65981632 None:intergenic 30.0%
!! CGGATTTTGATCGAAAATAA+GGG + chr3.4:65982633-65982652 None:intergenic 30.0%
!! GGAATCGAAACATTTTAAGA+AGG - chr3.4:65983175-65983194 MS.gene05042:CDS 30.0%
!! TGAAAAGTATCTGACACTAA+AGG - chr3.4:65982777-65982796 MS.gene05042:CDS 30.0%
!!! CATATGTGATTTGGTTTTGA+AGG - chr3.4:65983296-65983315 MS.gene05042:CDS 30.0%
!!! GATCGAAAATAAGGGTTTTT+TGG + chr3.4:65982625-65982644 None:intergenic 30.0%
AAAATTCTCAAGGAAAGCAG+TGG - chr3.4:65982815-65982834 MS.gene05042:CDS 35.0%
AAATTCTCAAGGAAAGCAGT+GGG - chr3.4:65982816-65982835 MS.gene05042:CDS 35.0%
AATACGGAACTTCAAAAGGA+GGG + chr3.4:65983680-65983699 None:intergenic 35.0%
ACAGTAACTCACAACAGAAA+GGG + chr3.4:65983244-65983263 None:intergenic 35.0%
CAATGACAGAAAAGGAAGTA+AGG - chr3.4:65983153-65983172 MS.gene05042:CDS 35.0%
CTACTTCTATGAAAAGTTCC+AGG - chr3.4:65984030-65984049 MS.gene05042:CDS 35.0%
GTCTCAGTTAAGTACATGTT+TGG + chr3.4:65982146-65982165 None:intergenic 35.0%
TAATACGGAACTTCAAAAGG+AGG + chr3.4:65983681-65983700 None:intergenic 35.0%
TTCAAGCTCCAAAATTCTCA+AGG - chr3.4:65982805-65982824 MS.gene05042:CDS 35.0%
! ACTGCTTTCCTTGAGAATTT+TGG + chr3.4:65982816-65982835 None:intergenic 35.0%
! ATTTCTTTTGTGTGATCTGG+AGG + chr3.4:65981610-65981629 None:intergenic 35.0%
! ATTTTCGATCAAAATCCGTC+AGG - chr3.4:65982635-65982654 MS.gene05042:CDS 35.0%
! CATGTTTGGAATCATTGTTG+TGG + chr3.4:65982132-65982151 None:intergenic 35.0%
! CATTTTCTATCTCTCTCTCT+TGG - chr3.4:65981639-65981658 MS.gene05042:CDS 35.0%
! CTGGAACTTTTCATAGAAGT+AGG + chr3.4:65984032-65984051 None:intergenic 35.0%
! GCAGTAAGGATTTTACTTTC+TGG - chr3.4:65982667-65982686 MS.gene05042:CDS 35.0%
! TCTTTTAATGTCTGAACCGT+TGG - chr3.4:65983057-65983076 MS.gene05042:CDS 35.0%
! TGGATTCGGTAAATTTGCAA+GGG - chr3.4:65982891-65982910 MS.gene05042:intron 35.0%
! TTTCTTTTGTGTGATCTGGA+GGG + chr3.4:65981609-65981628 None:intergenic 35.0%
! TTTTCGATCAAAATCCGTCA+GGG - chr3.4:65982636-65982655 MS.gene05042:CDS 35.0%
!! TTCCTTTTCTGTCATTGACT+GGG + chr3.4:65983150-65983169 None:intergenic 35.0%
!!! GTTTTTTGGGTTGAATTCAC+TGG + chr3.4:65982611-65982630 None:intergenic 35.0%
!!! TGAGAATTTTGGAGCTTGAA+CGG + chr3.4:65982805-65982824 None:intergenic 35.0%
AAAGGAGGGTAACATGTTTG+AGG + chr3.4:65983666-65983685 None:intergenic 40.0%
ACTGTTGCCAATTGTTGCTA+AGG + chr3.4:65982399-65982418 None:intergenic 40.0%
AGAAGGAACATACTGCACAA+TGG + chr3.4:65982749-65982768 None:intergenic 40.0%
ATAACTCGAAACTGTGACGT+TGG - chr3.4:65983321-65983340 MS.gene05042:CDS 40.0%
ATGTGTTTGAGCTGATCAAG+AGG - chr3.4:65982941-65982960 MS.gene05042:intron 40.0%
GAAATGGGACTGAAGAAATG+TGG - chr3.4:65982361-65982380 MS.gene05042:intron 40.0%
GACAGTAACTCACAACAGAA+AGG + chr3.4:65983245-65983264 None:intergenic 40.0%
GCTGTAACAAGTAGTACAAC+AGG + chr3.4:65982970-65982989 None:intergenic 40.0%
GGAGGAAAGGTCAATTCAAA+TGG + chr3.4:65982728-65982747 None:intergenic 40.0%
GTTATCACTGCAGAGAATTC+CGG - chr3.4:65983719-65983738 MS.gene05042:intron 40.0%
TTAACTGAGACTCTGAGTGA+GGG - chr3.4:65982155-65982174 MS.gene05042:intron 40.0%
TTGAAACAAGTGGCAGTCAA+GGG - chr3.4:65984054-65984073 MS.gene05042:CDS 40.0%
TTTCGATCAAAATCCGTCAG+GGG - chr3.4:65982637-65982656 MS.gene05042:CDS 40.0%
! ACTTGTTACAGCGATTTGCA+CGG - chr3.4:65982978-65982997 MS.gene05042:CDS 40.0%
! ATTTTCCAATCACGACCATC+GGG + chr3.4:65983801-65983820 None:intergenic 40.0%
! CATTTTCCAATCACGACCAT+CGG + chr3.4:65983802-65983821 None:intergenic 40.0%
! CTTCCTTTTCTGTCATTGAC+TGG + chr3.4:65983151-65983170 None:intergenic 40.0%
! GTGGATTCGGTAAATTTGCA+AGG - chr3.4:65982890-65982909 MS.gene05042:intron 40.0%
! TTCTTTTGTGTGATCTGGAG+GGG + chr3.4:65981608-65981627 None:intergenic 40.0%
! TTTGGAATCATTGTTGTGGG+AGG + chr3.4:65982128-65982147 None:intergenic 40.0%
AGGAACATACTGCACAATGG+AGG + chr3.4:65982746-65982765 None:intergenic 45.0%
AGTTCCAGGGTTGAAACAAG+TGG - chr3.4:65984044-65984063 MS.gene05042:CDS 45.0%
ATTTCCCATTCACGACCATC+GGG + chr3.4:65982346-65982365 None:intergenic 45.0%
CATACTGCACAATGGAGGAA+AGG + chr3.4:65982741-65982760 None:intergenic 45.0%
CATTTCCCATTCACGACCAT+CGG + chr3.4:65982347-65982366 None:intergenic 45.0%
CTTAACTGAGACTCTGAGTG+AGG - chr3.4:65982154-65982173 MS.gene05042:intron 45.0%
GATGGTCGTGAATGGGAAAT+GGG - chr3.4:65982346-65982365 MS.gene05042:intron 45.0%
GCTAAGATTACTGTTCCCGA+TGG - chr3.4:65982328-65982347 MS.gene05042:intron 45.0%
GCTACAATTACAGTTCCCGA+TGG - chr3.4:65983783-65983802 MS.gene05042:intron 45.0%
GTTGAAACAAGTGGCAGTCA+AGG - chr3.4:65984053-65984072 MS.gene05042:CDS 45.0%
TACTGCATAAAAGCCCCTGA+CGG + chr3.4:65982653-65982672 None:intergenic 45.0%
TCTTTGTCATGAATGGGCGA+GGG + chr3.4:65983209-65983228 None:intergenic 45.0%
TGCCCAGTCAATGACAGAAA+AGG - chr3.4:65983145-65983164 MS.gene05042:CDS 45.0%
TGGCGGAAAACTTTGAGTCA+CGG - chr3.4:65982021-65982040 MS.gene05042:intron 45.0%
TTATTGCAATGCAGCCTCCA+CGG - chr3.4:65983114-65983133 MS.gene05042:CDS 45.0%
TTTGAATCTTTCTCCGGCTC+CGG + chr3.4:65981562-65981581 None:intergenic 45.0%
! TCACAAATGCGAATAACGGG+TGG - chr3.4:65982871-65982890 MS.gene05042:intron 45.0%
! TCAGGGGCTTTTATGCAGTA+AGG - chr3.4:65982653-65982672 MS.gene05042:CDS 45.0%
! TCTTTTGTGTGATCTGGAGG+GGG + chr3.4:65981607-65981626 None:intergenic 45.0%
!! ATGCGAATAACGGGTGGATT+CGG - chr3.4:65982877-65982896 MS.gene05042:intron 45.0%
ATGAATGGGCGAGGGAAGTA+TGG + chr3.4:65983201-65983220 None:intergenic 50.0%
CGATGGTCGTGAATGGGAAA+TGG - chr3.4:65982345-65982364 MS.gene05042:intron 50.0%
CTCTTTGTCATGAATGGGCG+AGG + chr3.4:65983210-65983229 None:intergenic 50.0%
GTGTCGGAACACATCTTCTC+CGG + chr3.4:65981534-65981553 None:intergenic 50.0%
TATTGCAATGCAGCCTCCAC+GGG - chr3.4:65983115-65983134 MS.gene05042:CDS 50.0%
TGTTCCCGATGGTCGTGAAT+GGG - chr3.4:65982339-65982358 MS.gene05042:intron 50.0%
! ACTGCCACTTGTTTCAACCC+TGG + chr3.4:65984051-65984070 None:intergenic 50.0%
! GCTCTTCTGGAAGCAGAGTT+AGG + chr3.4:65982577-65982596 None:intergenic 50.0%
! GGCAGCACATTTTGCTCTTC+TGG + chr3.4:65982590-65982609 None:intergenic 50.0%
ATGTGTTCCGACACCGTCAC+CGG - chr3.4:65981540-65981559 MS.gene05042:CDS 55.0%
ATTGCAATGCAGCCTCCACG+GGG - chr3.4:65983116-65983135 MS.gene05042:CDS 55.0%
CAGTTCCCGATGGTCGTGAT+TGG - chr3.4:65983793-65983812 MS.gene05042:intron 55.0%
CTGTTCCCGATGGTCGTGAA+TGG - chr3.4:65982338-65982357 MS.gene05042:intron 55.0%
TCTTTCTCCGGCTCCGGTGA+CGG + chr3.4:65981556-65981575 None:intergenic 60.0%
GACTGGGCAGACATTCCCCG+TGG + chr3.4:65983134-65983153 None:intergenic 65.0%
TGGGCAGACATTCCCCGTGG+AGG + chr3.4:65983131-65983150 None:intergenic 65.0%
TCCGACACCGTCACCGGAGC+CGG - chr3.4:65981546-65981565 MS.gene05042:CDS 70.0%
! TCCGGCTCCGGTGACGGTGT+CGG + chr3.4:65981550-65981569 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 65981520 65984100 65981520 ID=MS.gene05042
chr3.4 mRNA 65981520 65984100 65981520 ID=MS.gene05042.t1;Parent=MS.gene05042
chr3.4 exon 65983990 65984100 65983990 ID=MS.gene05042.t1.exon1;Parent=MS.gene05042.t1
chr3.4 CDS 65983990 65984100 65983990 ID=cds.MS.gene05042.t1;Parent=MS.gene05042.t1
chr3.4 exon 65982951 65983343 65982951 ID=MS.gene05042.t1.exon2;Parent=MS.gene05042.t1
chr3.4 CDS 65982951 65983343 65982951 ID=cds.MS.gene05042.t1;Parent=MS.gene05042.t1
chr3.4 exon 65982621 65982863 65982621 ID=MS.gene05042.t1.exon3;Parent=MS.gene05042.t1
chr3.4 CDS 65982621 65982863 65982621 ID=cds.MS.gene05042.t1;Parent=MS.gene05042.t1
chr3.4 exon 65982388 65982492 65982388 ID=MS.gene05042.t1.exon4;Parent=MS.gene05042.t1
chr3.4 CDS 65982388 65982492 65982388 ID=cds.MS.gene05042.t1;Parent=MS.gene05042.t1
chr3.4 exon 65981520 65981888 65981520 ID=MS.gene05042.t1.exon5;Parent=MS.gene05042.t1
chr3.4 CDS 65981520 65981888 65981520 ID=cds.MS.gene05042.t1;Parent=MS.gene05042.t1
Gene Sequence

>MS.gene05042

ATGAAGACTCGCCGGAGAAGATGTGTTCCGACACCGTCACCGGAGCCGGAGAAAGATTCAAAACATTTTATGAAATGCATACTTCCCCCTCCAGATCACACAAAAGAAATAGTATGTTTCATTTTCTATCTCTCTCTCTTGGATACTAAACTCTTATTATAGATAAATATTCAAAATTTGTATGAGGAAATAATGCTGCAAGTTTAAACCTCTCCTGTAAATCAAATATTGTTGCTGCTGTTATTTCTTATTATTGTTGTTGGTTGTTTTTATGTTTGGTTCTAAGTTTGTAAAATATGATTTTGAATCTTTGATTGATTGGATTGAGTCTGTGAGTTCAACATTTTCATTTCTCAAGACATGTCAATGTGAGTGATTTTATAGATAAAGCTAGAAATTGGATCTTCTACAAACTAAGAGTGAGTTGAGTCGAAAGTGACTTATATTTGCTTCATATATAGTGATTTAAATAATAAATTTGAGCATAAAAATCATGTTTGATGGCGGAAAACTTTGAGTCACGGCAACAAGTTAGAATCTATTCTATAGTAAAAAGCAATGCTGCCTAAACGTTTCAATCAATTTCGTATCTGAAATCACTTTTGCCTCCCACAACAATGATTCCAAACATGTACTTAACTGAGACTCTGAGTGAGGGTAGTTACTTAAACTGTAGAATTCTCATAAATGTGTCACCCCTCTTTTGAAGTTTGATTGATCCTATAGTAGTATAACTGATGAGCTGTTAACACTGCAGCGAATTCCAGACGAGTTTATAAAAAGATATGGGAACGAACTCAAGAATGTTGCTAAGATTACTGTTCCCGATGGTCGTGAATGGGAAATGGGACTGAAGAAATGTGGCGAACATGTTTTCCTTAGCAACAATTGGCAACAGTTTGTAGAATACTATTCTATTTATTATGGTTGTTATTTAGATTTCAAGTATGAAGGGTATTCAAAGTTTAATGTTGTTATATATGATACAACTTCTGTTGAGATTTCTTATCCATTCAAAACTTCAAGTACTAATAGTGAGAAAAACATAAAGGGTCCTAACTCTGCTTCCAGAAGAGCAAAATGTGCTGCCAGTGAATTCAACCCAAAAAACCCTTATTTTCGATCAAAATCCGTCAGGGGCTTTTATGCAGTAAGGATTTTACTTTCTGGTTTTTAAAGTTACGGTATGATTTATATTTATGGTGCCATTTGAATTGACCTTTCCTCCATTGTGCAGTATGTTCCTTCTAGTTTTGCTGAAAAGTATCTGACACTAAAGGTTCCGTTCAAGCTCCAAAATTCTCAAGGAAAGCAGTGGGAAGTTTATTGTGTATTGCATGAAAAAGGAAAATCACAAATGCGAATAACGGGTGGATTCGGTAAATTTGCAAGGGAAAACAATCTATTAAAGGGAGTTACATATGTGTTTGAGCTGATCAAGAGGAAGCCTGTTGTACTACTTGTTACAGCGATTTGCACGGTTAAAAAATCTTCGACATTTGTTTATTCATCAATTCTAATTTCTTTCAAAGTTTTTTCTTTTAATGTCTGAACCGTTGGTGAACTTAATGCATAAAGTGATAATAGTTATTGCTTATTGCAATGCAGCCTCCACGGGGAATGTCTGCCCAGTCAATGACAGAAAAGGAAGTAAGGGAATCGAAACATTTTAAGAAGGCCATACTTCCCTCGCCCATTCATGACAAAGAGATAGTAAGTTTCCCTTTCTGTTGTGAGTTACTGTCATTTTTTGCTTTCAGCTAAATAGTAAAAAAGCATATGTGATTTGGTTTTGAAGGTAATAACTCGAAACTGTGACGTTGGATTGTCTATGTCTATGATCTGCTAAATAATAAAAAACCATAATAAGAAGAATAAAAAAATTCAGTGATGAGAAAAACAGCATGAAAATATTCAGCTTTGGATTTGTCTATGTCTCTGATTTGGTTTGAAGTTAATAACTCAAAACTGTGACTTTAGATTGTTAAAGACAAAATTTCCAAATACTACCTTTCAAGTTAGAACTGAATCTAGGCAAAAACAATTCTACTTGAGAACACTCGAACATGTCCAAATCAAATGTATGTGTCTAGAATCATTTTTTTCCCTTGAAAAACTACCTTTAAACATCAAATTAGGGAAACCTCAAACATGTTACCCTCCTTTTGAAGTTCCGTATTATAGTATAACAGATGAGCTGTTATCACTGCAGAGAATTCCGGATGAGTTCATAACAAGATTTGGGAACGAACTCAAGAATGTTGCTACAATTACAGTTCCCGATGGTCGTGATTGGAAAATGAGATTGAAGAAACGCGACAATGACATTTTCTTTTACAACGAATGGCAAGAGTTTGCAAATTACTATTCGTTAGGATATGGTTGCTTTCTGTCTTTCAAATATGAAGGGAATTCAATGTTTAGTGTTATTGTATTTGATGTAACTTCGGTTGAGATTTGCTATCCACTCAAAAATCTAAGTACTAATGGAGAAACAAACACACAGTGTCCTACTTCTATGAAAAGTTCCAGGGTTGAAACAAGTGGCAGTCAAGGGAAAAAAGCTCAAAATCATGTTTGA
Protein sequence

>MS.gene05042.t1

MKTRRRRCVPTPSPEPEKDSKHFMKCILPPPDHTKEIRIPDEFIKRYGNELKNVAKITVPDGREWEMGLKKCGEHVFLSNNWQQFVEYYSIYYGCYLDFKYEGYSKFNVVIYDTTSVEISYPFKTSSTNSEKNIKGPNSASRRAKCAASEFNPKNPYFRSKSVRGFYAYVPSSFAEKYLTLKVPFKLQNSQGKQWEVYCVLHEKGKSQMRITGGFGKFARENNLLKGVTYVFELIKRKPVVLLVTAICTPPRGMSAQSMTEKEVRESKHFKKAILPSPIHDKEIRIPDEFITRFGNELKNVATITVPDGRDWKMRLKKRDNDIFFYNEWQEFANYYSLGYGCFLSFKYEGNSMFSVIVFDVTSVEICYPLKNLSTNGETNTQCPTSMKSSRVETSGSQGKKAQNHV