AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050453

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050453.t1 MTR_3g052770 95.000 180 4 2 10 189 75 249 2.19e-124 352
MS.gene050453.t1 MTR_4g104230 60.335 179 66 2 11 189 81 254 8.30e-79 236
MS.gene050453.t1 MTR_4g104230 60.335 179 66 2 11 189 80 253 9.48e-79 236
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050453.t1 AT5G63280 60.556 180 66 2 10 189 74 248 7.60e-73 221
MS.gene050453.t1 AT5G40710 55.556 180 74 3 10 189 76 249 4.42e-71 216
MS.gene050453.t1 AT5G40710 58.730 126 47 2 10 135 76 196 6.57e-49 159

Find 34 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACACTCCAATTTACTTAAT+TGG 0.146499 3.4:+49613641 MS.gene050453:CDS
TGTGACCATGAAATTAATTC+TGG 0.266563 3.4:+49614647 MS.gene050453:CDS
ATAATACCAATTAAGTAAAT+TGG 0.284783 3.4:-49613647 None:intergenic
CCTGCAGCGAGCCGGCTTCA+TGG 0.327309 3.4:+49614836 MS.gene050453:CDS
GCTGCAGGACCCTCGTTAAC+TGG 0.349350 3.4:-49614821 None:intergenic
TTGACCCAGAATTAATTTCA+TGG 0.350995 3.4:-49614652 None:intergenic
GTCAAATTACCTGCGCCCTC+TGG 0.405967 3.4:-49615006 None:intergenic
ATATTTCTTGCTGCAGCAGC+AGG 0.422467 3.4:-49614692 None:intergenic
TCTCTATATATATCATTGTC+AGG 0.427562 3.4:-49613514 None:intergenic
CCATGAAGCCGGCTCGCTGC+AGG 0.478890 3.4:-49614836 None:intergenic
TGTGATGCTCACACCTGCAC+TGG 0.492800 3.4:+49614966 MS.gene050453:CDS
GTGACCATGAAATTAATTCT+GGG 0.499220 3.4:+49614648 MS.gene050453:CDS
GCTCACACCTGCACTGGACG+TGG 0.502873 3.4:+49614972 MS.gene050453:CDS
AGGCTTTCCACGTCCAGTGC+AGG 0.511555 3.4:-49614979 None:intergenic
CTCATATAGATATAAATGAA+TGG 0.514723 3.4:+49613554 MS.gene050453:CDS
CAATGATATATATAGAGATC+AGG 0.516644 3.4:+49613519 MS.gene050453:CDS
TACCTGCGCCCTCTGGAGAA+AGG 0.523388 3.4:-49614999 None:intergenic
ACGAGGGTCCTGCAGCGAGC+CGG 0.531828 3.4:+49614828 MS.gene050453:CDS
ATAATATCTCAAGAAGGTGC+AGG 0.547108 3.4:+49613485 MS.gene050453:CDS
TCTACATGTATTTATATCAA+AGG 0.559173 3.4:+49615976 MS.gene050453:CDS
TGGAAAACAACTATCAGCTA+TGG 0.566317 3.4:-49614801 None:intergenic
CAAGAAATATGCATATATTG+AGG 0.566436 3.4:+49614705 MS.gene050453:CDS
CACATACATCTGCTAAGCAC+CGG 0.569311 3.4:-49614613 None:intergenic
AGCCTTTCTCCAGAGGGCGC+AGG 0.580356 3.4:+49614997 MS.gene050453:CDS
CTCTATATATATCATTGTCA+GGG 0.590660 3.4:-49613513 None:intergenic
ATATTGAAATACCATGAAGC+CGG 0.593494 3.4:-49614847 None:intergenic
TTGCAGCATCTGAATCTACA+TGG 0.597085 3.4:-49612796 None:intergenic
GAAAATATAATATCTCAAGA+AGG 0.599633 3.4:+49613479 MS.gene050453:intron
CGGTGCTTAGCAGATGTATG+TGG 0.600256 3.4:+49614614 MS.gene050453:CDS
ATGTAGATTCAGATGCTGCA+AGG 0.607887 3.4:+49612798 MS.gene050453:CDS
CGTGGAAAGCCTTTCTCCAG+AGG 0.614403 3.4:+49614990 MS.gene050453:CDS
GTGGAAAGCCTTTCTCCAGA+GGG 0.645090 3.4:+49614991 MS.gene050453:CDS
GCTGAATCCAGAAAGAAAGC+CGG 0.646951 3.4:+49614594 MS.gene050453:intron
GATATAAATGAATGGCAGTG+TGG 0.647971 3.4:+49613562 MS.gene050453:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTCTTTTAAAATTTCT+AGG - chr3.4:49614411-49614430 None:intergenic 10.0%
!!! AATAATAATTTTAGAAATAG+AGG - chr3.4:49613978-49613997 None:intergenic 10.0%
!!! ATTTTTTGGAAATATTAATA+TGG + chr3.4:49613819-49613838 MS.gene050453:intron 10.0%
!!! GAAATTTTAAAAGAATTTTA+AGG + chr3.4:49614412-49614431 MS.gene050453:intron 10.0%
!!! TCTATTTCTAAAATTATTAT+TGG + chr3.4:49613977-49613996 MS.gene050453:intron 10.0%
!! ATAATACCAATTAAGTAAAT+TGG - chr3.4:49613650-49613669 None:intergenic 15.0%
!! TAAATATCATTGTTTAAACT+AGG + chr3.4:49614318-49614337 MS.gene050453:intron 15.0%
!!! AAAATATTTTTACCTTTTCT+TGG + chr3.4:49613149-49613168 MS.gene050453:intron 15.0%
!!! ATTAATATGGTTATTTTATG+AGG + chr3.4:49613832-49613851 MS.gene050453:intron 15.0%
!!! GAGAATTAGAATATAATTTT+AGG - chr3.4:49614767-49614786 None:intergenic 15.0%
!!! TAAAATTGTATTGATTCTAT+TGG - chr3.4:49613681-49613700 None:intergenic 15.0%
!! ATTAACTATATCATGAATGA+TGG - chr3.4:49613790-49613809 None:intergenic 20.0%
!! CTACATGTATTTATATCAAA+GGG + chr3.4:49615977-49615996 MS.gene050453:CDS 20.0%
!! CTCATATAGATATAAATGAA+TGG + chr3.4:49613554-49613573 MS.gene050453:CDS 20.0%
!! GAAAATATAATATCTCAAGA+AGG + chr3.4:49613479-49613498 MS.gene050453:intron 20.0%
!! TATTAAATGATGTCAAATAG+TGG + chr3.4:49615143-49615162 MS.gene050453:intron 20.0%
!! TCTACATGTATTTATATCAA+AGG + chr3.4:49615976-49615995 MS.gene050453:CDS 20.0%
!! TTGCAGAATTTGTATATTTA+TGG + chr3.4:49614883-49614902 MS.gene050453:intron 20.0%
!!! ACTGTTTTTATAAGCTATAT+TGG - chr3.4:49615568-49615587 None:intergenic 20.0%
!!! TAAACCTTCTGAAAAAATTT+CGG + chr3.4:49614512-49614531 MS.gene050453:intron 20.0%
!!! TTTCCAGAAATCTTTATTTT+TGG + chr3.4:49615303-49615322 MS.gene050453:intron 20.0%
! AATGTTATTGTTCAGAATTC+TGG + chr3.4:49613224-49613243 MS.gene050453:intron 25.0%
! ACTAAAGTAAACAAACACAT+TGG + chr3.4:49612997-49613016 MS.gene050453:intron 25.0%
! ACTAGTTATTTGATCAACAA+TGG - chr3.4:49613713-49613732 None:intergenic 25.0%
! AGAAATAATCATGTATCAAG+AGG - chr3.4:49613431-49613450 None:intergenic 25.0%
! AGAATTCTGTCAAATTGTTA+AGG - chr3.4:49614932-49614951 None:intergenic 25.0%
! AGGATAAACTTGTAAAACAA+TGG + chr3.4:49615414-49615433 MS.gene050453:intron 25.0%
! ATGTTCTTAAATATTAGGCT+TGG - chr3.4:49614141-49614160 None:intergenic 25.0%
! CAAGAAATATGCATATATTG+AGG + chr3.4:49614705-49614724 MS.gene050453:CDS 25.0%
! CAAGCATGTTCTTAAATATT+AGG - chr3.4:49614146-49614165 None:intergenic 25.0%
! CAATGATATATATAGAGATC+AGG + chr3.4:49613519-49613538 MS.gene050453:CDS 25.0%
! CTCTATATATATCATTGTCA+GGG - chr3.4:49613516-49613535 None:intergenic 25.0%
! GATGATTCTATTATCATCAA+TGG + chr3.4:49612911-49612930 MS.gene050453:intron 25.0%
! GTTATTGAATGTAGCTATAT+AGG + chr3.4:49613067-49613086 MS.gene050453:intron 25.0%
! TATCCAATATTGATGTATGT+CGG - chr3.4:49613391-49613410 None:intergenic 25.0%
! TCAATTTCTTTACTTCTTAG+TGG - chr3.4:49613045-49613064 None:intergenic 25.0%
! TCACCAAAAATAAAGATTTC+TGG - chr3.4:49615309-49615328 None:intergenic 25.0%
! TCTCTATATATATCATTGTC+AGG - chr3.4:49613517-49613536 None:intergenic 25.0%
! TGAAATCAAGAGTCATTAAT+TGG + chr3.4:49615868-49615887 MS.gene050453:intron 25.0%
! TGACATCATTTAATACAAAG+AGG - chr3.4:49615138-49615157 None:intergenic 25.0%
! TGTAAAACAATGGACATATT+TGG + chr3.4:49615424-49615443 MS.gene050453:intron 25.0%
! TTGGAGAACTTAATGAAAAT+AGG - chr3.4:49615549-49615568 None:intergenic 25.0%
! TTGTGTAGATAATACTATGA+TGG + chr3.4:49615365-49615384 MS.gene050453:intron 25.0%
! TTGTTAAATAGATGCTATAG+TGG + chr3.4:49615063-49615082 MS.gene050453:intron 25.0%
! TTGTTGATCAAATAACTAGT+TGG + chr3.4:49613713-49613732 MS.gene050453:intron 25.0%
! TTTAAGGGAAGTAAAATTTC+AGG - chr3.4:49614259-49614278 None:intergenic 25.0%
!! TAATACAAAGAGGCATATTA+CGG - chr3.4:49615128-49615147 None:intergenic 25.0%
!! TTAAAATTTCTAGGTCAACA+GGG - chr3.4:49614402-49614421 None:intergenic 25.0%
!! TTAAACTAGGCTAAGTTTTA+AGG + chr3.4:49614331-49614350 MS.gene050453:intron 25.0%
!! TTACCTTTTCTTGGTTTATA+TGG + chr3.4:49613158-49613177 MS.gene050453:intron 25.0%
!! TTGGATTAGATTGAAGTAAT+CGG + chr3.4:49613760-49613779 MS.gene050453:intron 25.0%
!! TTTAAAATTTCTAGGTCAAC+AGG - chr3.4:49614403-49614422 None:intergenic 25.0%
!!! AATCCGTTGATAATTGATAT+TGG - chr3.4:49615729-49615748 None:intergenic 25.0%
!!! ATCCGTTGATAATTGATATT+GGG - chr3.4:49615728-49615747 None:intergenic 25.0%
!!! CATTTGAGTCATTTTATAGT+TGG - chr3.4:49613341-49613360 None:intergenic 25.0%
!!! CTTTTTTCAATTAGAAGTGT+CGG + chr3.4:49613123-49613142 MS.gene050453:intron 25.0%
!!! GAAATCTTTATTTTTGGTGA+AGG + chr3.4:49615309-49615328 MS.gene050453:intron 25.0%
!!! GGAATTAAAAAAGTTTGTTG+TGG - chr3.4:49615757-49615776 None:intergenic 25.0%
AAACGGAAAAAAAAACCAGT+TGG + chr3.4:49613741-49613760 MS.gene050453:intron 30.0%
AAACTACAGCAATCAAACAA+AGG - chr3.4:49614371-49614390 None:intergenic 30.0%
AAGCCATATAAACCAAGAAA+AGG - chr3.4:49613164-49613183 None:intergenic 30.0%
AATAAGTTGTTTATCCACAC+AGG + chr3.4:49615702-49615721 MS.gene050453:intron 30.0%
AATAATACCGAAACAAGTTG+TGG + chr3.4:49614194-49614213 MS.gene050453:intron 30.0%
ATAAGTTTCCTGAGACTATT+TGG + chr3.4:49615475-49615494 MS.gene050453:intron 30.0%
ATATTGAAATACCATGAAGC+CGG - chr3.4:49614850-49614869 None:intergenic 30.0%
ATTAGATTGAAGTAATCGGA+TGG + chr3.4:49613764-49613783 MS.gene050453:intron 30.0%
CAACAACATCACAAGAAAAA+TGG - chr3.4:49615943-49615962 None:intergenic 30.0%
GACACTCCAATTTACTTAAT+TGG + chr3.4:49613641-49613660 MS.gene050453:CDS 30.0%
GATGATGCTAAATATGAAAG+TGG - chr3.4:49614288-49614307 None:intergenic 30.0%
GGAAGTAAAATTTCAGGATT+TGG - chr3.4:49614253-49614272 None:intergenic 30.0%
GTGACCATGAAATTAATTCT+GGG + chr3.4:49614648-49614667 MS.gene050453:CDS 30.0%
TAAGTTTCCTGAGACTATTT+GGG + chr3.4:49615476-49615495 MS.gene050453:intron 30.0%
TACAAGATACATGTAGAGTT+TGG - chr3.4:49615277-49615296 None:intergenic 30.0%
TCGATAGGATGTTATACTTA+GGG + chr3.4:49615787-49615806 MS.gene050453:intron 30.0%
TGTGACCATGAAATTAATTC+TGG + chr3.4:49614647-49614666 MS.gene050453:CDS 30.0%
TTAATTACAAGTGTCAGTGT+CGG - chr3.4:49613946-49613965 None:intergenic 30.0%
TTACTTCAATCTAATCCAAC+TGG - chr3.4:49613759-49613778 None:intergenic 30.0%
TTGACCCAGAATTAATTTCA+TGG - chr3.4:49614655-49614674 None:intergenic 30.0%
TTGATCAAATAACTAGTTGG+TGG + chr3.4:49613716-49613735 MS.gene050453:intron 30.0%
TTTAAGGATAAAACACCTGT+TGG + chr3.4:49614428-49614447 MS.gene050453:intron 30.0%
! ATTGTTTTCATGTTTTGTCC+AGG - chr3.4:49614177-49614196 None:intergenic 30.0%
! CATTTTTCTTGTGATGTTGT+TGG + chr3.4:49615941-49615960 MS.gene050453:CDS 30.0%
! GTTTTACAAGTTTATCCTTC+AGG - chr3.4:49615412-49615431 None:intergenic 30.0%
! TAAAATTTCTAGGTCAACAG+GGG - chr3.4:49614401-49614420 None:intergenic 30.0%
!! AGTCAGTTTTAATGTCTGAT+TGG + chr3.4:49612823-49612842 MS.gene050453:intron 30.0%
!! ATAACTAGTTGGTGGAAAAA+CGG + chr3.4:49613724-49613743 MS.gene050453:intron 30.0%
!! TGGTTATTTTATGAGGAACT+TGG + chr3.4:49613839-49613858 MS.gene050453:intron 30.0%
!!! ATGTCTGATTGGAAGTATTT+TGG + chr3.4:49612834-49612853 MS.gene050453:intron 30.0%
AACCTAATTATCTCTCCTGA+AGG + chr3.4:49615394-49615413 MS.gene050453:intron 35.0%
AATGTAGCTATATAGGTCAG+TGG + chr3.4:49613074-49613093 MS.gene050453:intron 35.0%
ATCCTTCAGGAGAGATAATT+AGG - chr3.4:49615399-49615418 None:intergenic 35.0%
ATTCCAGTGATTTAGTCGAT+AGG + chr3.4:49615772-49615791 MS.gene050453:intron 35.0%
ATTGTTCAGAATTCTGGATC+AGG + chr3.4:49613230-49613249 MS.gene050453:intron 35.0%
CAACATCACAAGAAAAATGG+AGG - chr3.4:49615940-49615959 None:intergenic 35.0%
CTCTGCTCAATTTATCGTAA+TGG + chr3.4:49614542-49614561 MS.gene050453:intron 35.0%
GAGAAAGAAGTAGAATAGCT+TGG + chr3.4:49613286-49613305 MS.gene050453:intron 35.0%
GATATAAATGAATGGCAGTG+TGG + chr3.4:49613562-49613581 MS.gene050453:CDS 35.0%
GGATACAATTCCTGTGAAAT+TGG + chr3.4:49615808-49615827 MS.gene050453:intron 35.0%
GGCCCAATATCAATTATCAA+CGG + chr3.4:49615723-49615742 MS.gene050453:intron 35.0%
GTCGATAGGATGTTATACTT+AGG + chr3.4:49615786-49615805 MS.gene050453:intron 35.0%
TGACCGACATACATCAATAT+TGG + chr3.4:49613385-49613404 MS.gene050453:intron 35.0%
TGCTAACTATTTCCTTGAGA+AGG + chr3.4:49614081-49614100 MS.gene050453:intron 35.0%
TGGAAAACAACTATCAGCTA+TGG - chr3.4:49614804-49614823 None:intergenic 35.0%
! ACTAACTCCACAACTTGTTT+CGG - chr3.4:49614204-49614223 None:intergenic 35.0%
! ATAATATCTCAAGAAGGTGC+AGG + chr3.4:49613485-49613504 MS.gene050453:CDS 35.0%
! CTAATTGAAAAAAGCGTGTC+AGG - chr3.4:49613117-49613136 None:intergenic 35.0%
! TCTAAGCTGATAGTGTATGA+AGG + chr3.4:49613879-49613898 MS.gene050453:intron 35.0%
!! AGTTGTTTTCCAGTTAACGA+GGG + chr3.4:49614812-49614831 MS.gene050453:CDS 35.0%
!! ATGAAAGTGGTGTTGCTTTA+AGG - chr3.4:49614275-49614294 None:intergenic 35.0%
!! TAGTTGTTTTCCAGTTAACG+AGG + chr3.4:49614811-49614830 MS.gene050453:CDS 35.0%
!! TCAACAATGAAGCATTGACA+CGG - chr3.4:49614037-49614056 None:intergenic 35.0%
!! TGAAAGTGGTGTTGCTTTAA+GGG - chr3.4:49614274-49614293 None:intergenic 35.0%
!!! GCTACCGAAATTTTTTCAGA+AGG - chr3.4:49614519-49614538 None:intergenic 35.0%
ACAACTGCAACCAATTTCAC+AGG - chr3.4:49615821-49615840 None:intergenic 40.0%
ATGTAGATTCAGATGCTGCA+AGG + chr3.4:49612798-49612817 MS.gene050453:CDS 40.0%
ATGTCAAATAGTGGCTAACG+TGG + chr3.4:49615152-49615171 MS.gene050453:intron 40.0%
GCAGTATTTGACGACATTTG+TGG + chr3.4:49613451-49613470 MS.gene050453:intron 40.0%
TAACCACAAATCACTCCAAC+AGG - chr3.4:49614446-49614465 None:intergenic 40.0%
TAATTGATATTGGGCCTGTG+TGG - chr3.4:49615719-49615738 None:intergenic 40.0%
TCAAATTCCGCTACACAACA+GGG - chr3.4:49615186-49615205 None:intergenic 40.0%
TTCAAATTCCGCTACACAAC+AGG - chr3.4:49615187-49615206 None:intergenic 40.0%
TTGCAGCATCTGAATCTACA+TGG - chr3.4:49612799-49612818 None:intergenic 40.0%
TTTGCAGCTTATAGCACAAG+TGG - chr3.4:49615684-49615703 None:intergenic 40.0%
! ATGCCTTCAATCTGAGGTAT+CGG - chr3.4:49613906-49613925 None:intergenic 40.0%
! CATCCTATCGACTAAATCAC+TGG - chr3.4:49615778-49615797 None:intergenic 40.0%
! CTTGTGCTTTTCTTGCTTGT+CGG + chr3.4:49613181-49613200 MS.gene050453:intron 40.0%
! CTTTTCTTGCTTGTCGGAAT+TGG + chr3.4:49613187-49613206 MS.gene050453:intron 40.0%
!! TTGTCATAAGCCGTTTTCAC+AGG - chr3.4:49615506-49615525 None:intergenic 40.0%
!!! CTGCTTTTTGCTTAGCTGTA+AGG + chr3.4:49615844-49615863 MS.gene050453:intron 40.0%
AAGGCATGTCTGACATGTGT+CGG + chr3.4:49613919-49613938 MS.gene050453:intron 45.0%
ACACCTGTTGGAGTGATTTG+TGG + chr3.4:49614440-49614459 MS.gene050453:intron 45.0%
AGCTCAGAAAAGTCCCAAGA+TGG - chr3.4:49614109-49614128 None:intergenic 45.0%
AGCTTGGAAGATCGTTCAAG+AGG + chr3.4:49613302-49613321 MS.gene050453:intron 45.0%
ATATTTCTTGCTGCAGCAGC+AGG - chr3.4:49614695-49614714 None:intergenic 45.0%
ATCTGTGGTTGACGACACTT+TGG + chr3.4:49615226-49615245 MS.gene050453:intron 45.0%
CACATACATCTGCTAAGCAC+CGG - chr3.4:49614616-49614635 None:intergenic 45.0%
CTTGCTTGTCGGAATTGGTA+TGG + chr3.4:49613192-49613211 MS.gene050453:intron 45.0%
GCTGAATCCAGAAAGAAAGC+CGG + chr3.4:49614594-49614613 MS.gene050453:intron 45.0%
GTTATAGCCCTGTTGTGTAG+CGG + chr3.4:49615176-49615195 MS.gene050453:intron 45.0%
TCAGACATGCCTTCAATCTG+AGG - chr3.4:49613912-49613931 None:intergenic 45.0%
TTTGGGAGAGCCTGTGAAAA+CGG + chr3.4:49615493-49615512 MS.gene050453:intron 45.0%
!! CCTTGAGAAGGTACCATCTT+GGG + chr3.4:49614093-49614112 MS.gene050453:intron 45.0%
!! TCCTTGAGAAGGTACCATCT+TGG + chr3.4:49614092-49614111 MS.gene050453:intron 45.0%
!! TGAAGCATTGACACGGACAT+TGG - chr3.4:49614030-49614049 None:intergenic 45.0%
!!! GTGCTATTTTCTGCGATCTG+TGG + chr3.4:49615211-49615230 MS.gene050453:intron 45.0%
!!! TTAATAAATGTTATATTTTT+TGG + chr3.4:49613805-49613824 MS.gene050453:intron 5.0%
AACATGCTTGAGTCGTGTCC+TGG + chr3.4:49614156-49614175 MS.gene050453:intron 50.0%
AGGCTCTCCCAAATAGTCTC+AGG - chr3.4:49615486-49615505 None:intergenic 50.0%
ATTCTGGATCAGGAGCAGAG+TGG + chr3.4:49613240-49613259 MS.gene050453:intron 50.0%
CCCAAGATGGTACCTTCTCA+AGG - chr3.4:49614096-49614115 None:intergenic 50.0%
CGGTGCTTAGCAGATGTATG+TGG + chr3.4:49614614-49614633 MS.gene050453:CDS 50.0%
GGACCGATACCTCAGATTGA+AGG + chr3.4:49613900-49613919 MS.gene050453:intron 50.0%
GTGGAAAGCCTTTCTCCAGA+GGG + chr3.4:49614991-49615010 MS.gene050453:CDS 50.0%
!! CTAAGCACCGGCTTTCTTTC+TGG - chr3.4:49614604-49614623 None:intergenic 50.0%
CGTGGAAAGCCTTTCTCCAG+AGG + chr3.4:49614990-49615009 MS.gene050453:CDS 55.0%
GTCAAATTACCTGCGCCCTC+TGG - chr3.4:49615009-49615028 None:intergenic 55.0%
TGTGATGCTCACACCTGCAC+TGG + chr3.4:49614966-49614985 MS.gene050453:CDS 55.0%
AGGCTTTCCACGTCCAGTGC+AGG - chr3.4:49614982-49615001 None:intergenic 60.0%
GCTGCAGGACCCTCGTTAAC+TGG - chr3.4:49614824-49614843 None:intergenic 60.0%
! TACCTGCGCCCTCTGGAGAA+AGG - chr3.4:49615002-49615021 None:intergenic 60.0%
GCTCACACCTGCACTGGACG+TGG + chr3.4:49614972-49614991 MS.gene050453:CDS 65.0%
! AGCCTTTCTCCAGAGGGCGC+AGG + chr3.4:49614997-49615016 MS.gene050453:CDS 65.0%
ACGAGGGTCCTGCAGCGAGC+CGG + chr3.4:49614828-49614847 MS.gene050453:CDS 70.0%
! CCTGCAGCGAGCCGGCTTCA+TGG + chr3.4:49614836-49614855 MS.gene050453:CDS 70.0%
!! CCATGAAGCCGGCTCGCTGC+AGG - chr3.4:49614839-49614858 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 49612794 49615997 49612794 ID=MS.gene050453
chr3.4 mRNA 49612794 49615997 49612794 ID=MS.gene050453.t1;Parent=MS.gene050453
chr3.4 exon 49612794 49612819 49612794 ID=MS.gene050453.t1.exon1;Parent=MS.gene050453.t1
chr3.4 CDS 49612794 49612819 49612794 ID=cds.MS.gene050453.t1;Parent=MS.gene050453.t1
chr3.4 exon 49613480 49613675 49613480 ID=MS.gene050453.t1.exon2;Parent=MS.gene050453.t1
chr3.4 CDS 49613480 49613675 49613480 ID=cds.MS.gene050453.t1;Parent=MS.gene050453.t1
chr3.4 exon 49614605 49614730 49614605 ID=MS.gene050453.t1.exon3;Parent=MS.gene050453.t1
chr3.4 CDS 49614605 49614730 49614605 ID=cds.MS.gene050453.t1;Parent=MS.gene050453.t1
chr3.4 exon 49614800 49614857 49614800 ID=MS.gene050453.t1.exon4;Parent=MS.gene050453.t1
chr3.4 CDS 49614800 49614857 49614800 ID=cds.MS.gene050453.t1;Parent=MS.gene050453.t1
chr3.4 exon 49614946 49615018 49614946 ID=MS.gene050453.t1.exon5;Parent=MS.gene050453.t1
chr3.4 CDS 49614946 49615018 49614946 ID=cds.MS.gene050453.t1;Parent=MS.gene050453.t1
chr3.4 exon 49615908 49615997 49615908 ID=MS.gene050453.t1.exon6;Parent=MS.gene050453.t1
chr3.4 CDS 49615908 49615997 49615908 ID=cds.MS.gene050453.t1;Parent=MS.gene050453.t1
Gene Sequence

>MS.gene050453

GGCCATGTAGATTCAGATGCTGCAAGGTGAGTCAGTTTTAATGTCTGATTGGAAGTATTTTGGACTATGAGAACAGTATTATTAGTTGTATTTTCAGAGTTCACTACTTAATGTCTTGATGATTCTATTATCATCAATGGAGAAACGATAAATAACTTATGTGCATATGCTTTGTTCATTTTTCTTATTGTTTTCACTATGAAACTAAAGTAAACAAACACATTGGACACGACATTTACACGTAGACACCACTAAGAAGTAAAGAAATTGAATGTTATTGAATGTAGCTATATAGGTCAGTGGCGTGTCAGTGTTAGACACCTGACACGCTTTTTTCAATTAGAAGTGTCGGTGCAAAATATTTTTACCTTTTCTTGGTTTATATGGCTTGTGCTTTTCTTGCTTGTCGGAATTGGTATGGCTAATAGATAATGTTATTGTTCAGAATTCTGGATCAGGAGCAGAGTGGCGATCATCAAATACATTGCTCTAGAGAAAGAAGTAGAATAGCTTGGAAGATCGTTCAAGAGGTATTCAAATACTTCCAACTATAAAATGACTCAAATGAGTGACTTGCTTAATTCTACTCTTTGACCGACATACATCAATATTGGATAACTGATAAATGTGCGTTCCTCTTGATACATGATTATTTCTGCAGTATTTGACGACATTTGTGGAGAAAGAAAATATAATATCTCAAGAAGGTGCAGGCTTCACCCTGACAATGATATATATAGAGATCAGGAACAACATAAATCTCATATAGATATAAATGAATGGCAGTGTGGATACTGTAAGAAAACTTTCTACGAAGAGAAACATCTTGATCAACATTTCGACAACAGACACTCCAATTTACTTAATTGGTATTATGCTCTTGTCCAATAGAATCAATACAATTTTATTTTTTCTACCATTGTTGATCAAATAACTAGTTGGTGGAAAAACGGAAAAAAAAACCAGTTGGATTAGATTGAAGTAATCGGATGGCCATCATTCATGATATAGTTAATAAATGTTATATTTTTTGGAAATATTAATATGGTTATTTTATGAGGAACTTGGTGTTGTGAAAGTGTCGATCTAAGCTGATAGTGTATGAAGGACCGATACCTCAGATTGAAGGCATGTCTGACATGTGTCGGACCGACACTGACACTTGTAATTAAATACTATCACCTCTATTTCTAAAATTATTATTGGTGTCAACGTGTCAGCTCAGTATCGTATCCAATGTCCGTGTCAATGCTTCATTGTTGATAGTTGATAGCTGATAGTACTAAGTGCTAACTATTTCCTTGAGAAGGTACCATCTTGGGACTTTTCTGAGCTCGCAGAAAACCAAGCCTAATATTTAAGAACATGCTTGAGTCGTGTCCTGGACAAAACATGAAAACAATAATACCGAAACAAGTTGTGGAGTTAGTAAAGTTGAAATAGTTCATTTAGTGATCCAAATCCTGAAATTTTACTTCCCTTAAAGCAACACCACTTTCATATTTAGCATCATCTTTCAAGTTTTAAATATCATTGTTTAAACTAGGCTAAGTTTTAAGGATGCTGTCGAGAAACCTTTGTTTGATTGCTGTAGTTTGCTAGATCCCCTGTTGACCTAGAAATTTTAAAAGAATTTTAAGGATAAAACACCTGTTGGAGTGATTTGTGGTTAATGATGTTTTATATCACTTGTATAATTCAGTATGATGTGAAGTTGTTAAACCTTCTGAAAAAATTTCGGTAGCTTTCTCTGCTCAATTTATCGTAATGGAGCATTAATAACTGAAATGAGATAATTGTGCTGAATCCAGAAAGAAAGCCGGTGCTTAGCAGATGTATGTGGAGCACTGCACTGTGACCATGAAATTAATTCTGGGTCAAAAAAATCAAAGTGCAATCCTGCTGCTGCAGCAAGAAATATGCATATATTGAGGTGTGTGCTTTTGTTTTTGTCTATTCATTTGTGTTAACCTAAAATTATATTCTAATTCTCTATTTTTTTATAGACCATAGCTGATAGTTGTTTTCCAGTTAACGAGGGTCCTGCAGCGAGCCGGCTTCATGGTATTTCAATATTTTATTCATTCTTTTGCAGAATTTGTATATTTATGGAAATATATGACACTGCATTCTAACCTTAACAATTTGACAGAATTCTTCTTGCATCAATTCTGTGATGCTCACACCTGCACTGGACGTGGAAAGCCTTTCTCCAGAGGGCGCAGGTAATTTGACTATAATCTAAAACTAAGTTGTGTTTTCTGTCTTCTTGTTAAATAGATGCTATAGTGGCGCTATATCGTAGCGAAATTTGAACAAATCACTATTGTTCCGTAATATGCCTCTTTGTATTAAATGATGTCAAATAGTGGCTAACGTGGCGTTATAGCCCTGTTGTGTAGCGGAATTTGAATAAAGTGCTATTTTCTGCGATCTGTGGTTGACGACACTTTGGTTTTATCAGTTCTTGTGATAGTTTTCCAAACTCTACATGTATCTTGTATATTGATTTCCAGAAATCTTTATTTTTGGTGAAGGATTCAGAAGTTCTGAAGAACATACTTTGACAAATTGTGTAGATAATACTATGATGGAAACATAACCTAATTATCTCTCCTGAAGGATAAACTTGTAAAACAATGGACATATTTGGATTAGCTTATTTGAGCTTATCTACTTGCATAAGTTTCCTGAGACTATTTGGGAGAGCCTGTGAAAACGGCTTATGACAATTTTCATAAGCTTTTTTGAGCCTATTTTCATTAAGTTCTCCAATATAGCTTATAAAAACAGTTTATATCATATACAAAAACAACTTAACTTTGTTTTTATTATTTGTTATAAAAATAGCTTATGTAAGCTTATACATAAGCGCTTATCATGATAACCACTTGTGCTATAAGCTGCAAATAAGTTGTTTATCCACACAGGCCCAATATCAATTATCAACGGATTCAATACCACAACAAACTTTTTTAATTCCAGTGATTTAGTCGATAGGATGTTATACTTAGGGATACAATTCCTGTGAAATTGGTTGCAGTTGTCTACTGCTTTTTGCTTAGCTGTAAGGATGAAATCAAGAGTCATTAATTGGTTTATTCATTTGTGCAGAAAAAAGACAAATGTGTTCTACATAGTTGCCTCCATTTTTCTTGTGATGTTGTTGGTACTTTTCTATCTCTACATGTATTTATATCAAAG
Protein sequence

>MS.gene050453.t1

GHVDSDAARKYNISRRCRLHPDNDIYRDQEQHKSHIDINEWQCGYCKKTFYEEKHLDQHFDNRHSNLLNWYYALKESRCLADVCGALHCDHEINSGSKKSKCNPAAAARNMHILRCTIADSCFPVNEGPAASRLHEFFLHQFCDAHTCTGRGKPFSRGRRKKTNVFYIVASIFLVMLLVLFYLYMYLYQ