AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050550.t1 MTR_7g115530 96.825 441 12 1 1 439 1 441 0.0 875
MS.gene050550.t1 MTR_7g115530 96.012 326 11 1 1 324 1 326 0.0 647
MS.gene050550.t1 MTR_7g115530 96.346 301 9 1 1 299 1 301 0.0 599
MS.gene050550.t1 MTR_7g115530 94.526 274 13 1 1 272 1 274 0.0 535
MS.gene050550.t1 MTR_7g115530 95.185 270 11 1 1 268 1 270 0.0 532
MS.gene050550.t1 MTR_7g115530 94.872 273 12 1 13 283 13 285 0.0 523
MS.gene050550.t1 MTR_1g080330 54.680 406 130 9 47 437 19 385 1.87e-131 384
MS.gene050550.t1 MTR_1g080330 58.562 292 78 6 47 323 19 282 3.96e-101 303
MS.gene050550.t1 MTR_7g098250 43.885 417 200 9 1 388 26 437 1.63e-94 293
MS.gene050550.t1 MTR_1g053835 52.542 177 70 5 203 375 247 413 6.84e-50 175
MS.gene050550.t1 MTR_7g093030 53.147 143 66 1 215 357 211 352 9.22e-48 168
MS.gene050550.t1 MTR_2g027860 54.362 149 66 1 216 362 49 197 3.47e-47 165
MS.gene050550.t1 MTR_7g088070 45.714 175 87 2 182 349 9 182 1.72e-46 162
MS.gene050550.t1 MTR_7g088070 44.382 178 89 2 182 349 9 186 8.50e-46 160
MS.gene050550.t1 MTR_6g032990 51.974 152 57 3 212 347 38 189 6.10e-41 150
MS.gene050550.t1 MTR_5g041350 36.979 192 97 4 212 381 11 200 6.72e-35 133
MS.gene050550.t1 MTR_5g027440 48.936 141 60 3 216 344 25 165 4.15e-34 129
MS.gene050550.t1 MTR_2g086450 42.767 159 70 3 212 349 41 199 6.58e-34 131
MS.gene050550.t1 MTR_6g444980 38.372 172 95 3 201 364 20 188 7.62e-34 130
MS.gene050550.t1 MTR_4g081710 38.144 194 93 5 212 381 13 203 3.29e-33 128
MS.gene050550.t1 MTR_6g444980 40.690 145 77 2 201 339 20 161 1.50e-31 124
MS.gene050550.t1 MTR_1g053800 40.252 159 92 2 190 348 153 308 4.41e-30 119
MS.gene050550.t1 MTR_2g027800 44.604 139 67 3 216 350 39 171 1.47e-29 117
MS.gene050550.t1 MTR_2g027800 40.136 147 76 1 216 350 39 185 2.02e-28 114
MS.gene050550.t1 MTR_7g093030 56.471 85 36 1 215 299 211 294 1.06e-27 112
MS.gene050550.t1 MTR_1g090670 38.889 144 79 1 216 350 44 187 1.39e-27 111
MS.gene050550.t1 MTR_7g068600 42.857 126 69 1 216 338 13 138 9.62e-27 107
MS.gene050550.t1 MTR_1g053830 50.515 97 44 3 203 299 7 99 6.67e-25 98.6
MS.gene050550.t1 MTR_7g093010 40.625 160 78 5 188 341 191 339 1.07e-23 102
MS.gene050550.t1 MTR_2g023580 59.420 69 26 1 217 285 56 122 4.85e-20 89.4
MS.gene050550.t1 MTR_0223s0040 67.857 56 18 0 217 272 19 74 1.66e-19 88.6
MS.gene050550.t1 MTR_1g112370 66.071 56 19 0 217 272 23 78 4.40e-19 88.2
MS.gene050550.t1 MTR_2g084230 60.294 68 26 1 217 284 98 164 1.54e-18 86.3
MS.gene050550.t1 MTR_3g450310 57.895 57 24 0 216 272 19 75 1.88e-18 84.0
MS.gene050550.t1 MTR_2g084230 60.294 68 26 1 217 284 98 164 1.97e-18 87.0
MS.gene050550.t1 MTR_8g086410 53.247 77 32 1 217 289 110 186 3.00e-18 87.0
MS.gene050550.t1 MTR_8g086410 53.247 77 32 1 217 289 112 188 3.00e-18 87.0
MS.gene050550.t1 MTR_7g069660 62.500 56 21 0 217 272 72 127 3.32e-18 85.9
MS.gene050550.t1 MTR_8g066310 40.945 127 64 2 210 336 167 282 1.21e-17 84.3
MS.gene050550.t1 MTR_6g071625 56.164 73 30 1 217 287 203 275 1.70e-17 84.0
MS.gene050550.t1 MTR_1g021520 66.071 56 19 0 217 272 127 182 1.82e-17 82.8
MS.gene050550.t1 MTR_2g016220 64.286 56 20 0 217 272 90 145 1.84e-17 84.0
MS.gene050550.t1 MTR_2g016220 64.286 56 20 0 217 272 90 145 2.06e-17 84.0
MS.gene050550.t1 MTR_8g086410 64.815 54 19 0 217 270 112 165 4.39e-17 83.6
MS.gene050550.t1 MTR_5g029470 61.667 60 23 0 213 272 216 275 1.78e-16 81.3
MS.gene050550.t1 MTR_3g064840 61.667 60 23 0 213 272 271 330 3.25e-16 80.9
MS.gene050550.t1 MTR_2g016220 68.000 50 16 0 217 266 90 139 1.38e-15 78.6
MS.gene050550.t1 MTR_2g450070 54.930 71 30 2 212 282 209 277 2.12e-15 79.0
MS.gene050550.t1 MTR_2g450070 54.930 71 30 2 212 282 209 277 2.31e-15 79.0
MS.gene050550.t1 MTR_4g121020 46.739 92 40 2 216 299 111 201 1.32e-14 76.3
MS.gene050550.t1 MTR_4g121020 46.739 92 40 2 216 299 215 305 1.56e-14 76.3
MS.gene050550.t1 MTR_4g121020 46.739 92 40 2 216 299 215 305 1.59e-14 76.3
MS.gene050550.t1 MTR_3g106220 60.345 58 22 1 212 269 194 250 1.67e-13 72.8
MS.gene050550.t1 MTR_4g021790 53.030 66 28 2 216 281 168 230 1.78e-13 70.9
MS.gene050550.t1 MTR_4g021855 41.905 105 47 4 205 299 158 258 1.80e-13 71.2
MS.gene050550.t1 MTR_1g093080 54.839 62 28 0 216 277 211 272 2.33e-13 71.6
MS.gene050550.t1 MTR_4g086835 62.963 54 20 0 216 269 274 327 2.75e-13 71.6
MS.gene050550.t1 MTR_4g131600 55.932 59 25 1 211 269 196 253 4.33e-13 71.6
MS.gene050550.t1 MTR_4g113140 53.030 66 31 0 210 275 229 294 5.62e-13 70.9
MS.gene050550.t1 MTR_1g013170 53.623 69 31 1 212 280 187 254 6.20e-13 70.9
MS.gene050550.t1 MTR_4g064730 51.562 64 30 1 206 269 121 183 9.92e-13 69.3
MS.gene050550.t1 MTR_1g032570 62.963 54 19 1 216 269 131 183 1.15e-12 70.1
MS.gene050550.t1 MTR_1g032570 62.963 54 19 1 216 269 200 252 1.19e-12 70.1
MS.gene050550.t1 MTR_4g131570 51.562 64 30 1 212 275 199 261 1.37e-12 70.1
MS.gene050550.t1 MTR_4g131580 53.125 64 29 1 212 275 196 258 2.01e-12 69.7
MS.gene050550.t1 MTR_5g054300 58.929 56 23 0 214 269 196 251 2.02e-12 68.6
MS.gene050550.t1 MTR_5g017980 57.895 57 24 0 213 269 190 246 3.59e-12 67.8
MS.gene050550.t1 MTR_4g086835 62.963 54 19 1 216 269 274 326 6.54e-12 67.4
MS.gene050550.t1 MTR_3g102590 53.226 62 28 1 212 273 139 199 6.85e-12 65.5
MS.gene050550.t1 MTR_7g117705 45.882 85 41 2 212 292 187 270 9.48e-12 67.0
MS.gene050550.t1 MTR_2g034960 59.259 54 21 1 216 269 211 263 1.02e-11 67.4
MS.gene050550.t1 MTR_4g098870 36.735 98 61 1 175 272 289 385 1.88e-11 66.6
MS.gene050550.t1 MTR_4g098870 36.735 98 61 1 175 272 269 365 2.02e-11 66.2
MS.gene050550.t1 MTR_4g098870 36.735 98 61 1 175 272 289 385 2.33e-11 66.2
MS.gene050550.t1 MTR_4g098870 36.735 98 61 1 175 272 308 404 2.36e-11 66.2
MS.gene050550.t1 MTR_1g090670 33.028 109 64 1 251 350 4 112 2.60e-11 63.5
MS.gene050550.t1 MTR_4g098870 36.735 98 61 1 175 272 269 365 2.61e-11 65.9
MS.gene050550.t1 MTR_1g013180 49.296 71 35 1 211 281 337 406 3.00e-11 65.9
MS.gene050550.t1 MTR_7g089010 53.704 54 24 1 216 269 91 143 4.82e-11 63.9
MS.gene050550.t1 MTR_5g017980 57.895 57 23 1 213 269 190 245 8.71e-11 63.5
MS.gene050550.t1 MTR_1g093080 56.863 51 22 0 216 266 211 261 8.99e-11 62.8
MS.gene050550.t1 MTR_5g017980 57.895 57 23 1 213 269 189 244 9.31e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050550.t1 AT5G29000 45.789 380 169 9 3 360 14 378 1.40e-87 273
MS.gene050550.t1 AT5G29000 47.198 339 140 7 52 360 6 335 2.76e-84 263
MS.gene050550.t1 AT5G29000 47.198 339 140 7 52 360 6 335 2.76e-84 263
MS.gene050550.t1 AT3G04450 49.175 303 121 8 93 377 71 358 3.02e-79 251
MS.gene050550.t1 AT3G04450 49.175 303 121 8 93 377 71 358 3.02e-79 251
MS.gene050550.t1 AT3G04450 49.175 303 121 8 93 377 111 398 1.01e-78 251
MS.gene050550.t1 AT4G28610 52.778 252 106 10 106 345 105 355 1.10e-71 232
MS.gene050550.t1 AT2G20400 39.701 335 155 10 44 345 40 360 1.12e-51 179
MS.gene050550.t1 AT2G20400 39.881 336 154 11 44 345 40 361 1.48e-50 176
MS.gene050550.t1 AT2G01060 48.603 179 86 2 215 392 14 187 1.46e-48 167
MS.gene050550.t1 AT5G29000 39.203 301 149 8 3 284 14 299 5.30e-47 164
MS.gene050550.t1 AT3G13040 50.000 196 81 3 208 386 233 428 2.21e-46 166
MS.gene050550.t1 AT3G13040 50.000 196 81 3 208 386 233 428 2.21e-46 166
MS.gene050550.t1 AT3G13040 50.000 196 81 3 208 386 233 428 2.21e-46 166
MS.gene050550.t1 AT5G06800 49.405 168 79 2 185 347 156 322 4.68e-46 164
MS.gene050550.t1 AT5G06800 48.256 172 80 2 185 347 156 327 3.47e-45 161
MS.gene050550.t1 AT3G04450 45.320 203 89 5 93 282 111 304 3.65e-44 157
MS.gene050550.t1 AT3G24120 44.444 171 83 3 204 362 29 199 9.81e-40 144
MS.gene050550.t1 AT4G13640 51.408 142 55 3 216 344 37 177 2.13e-39 144
MS.gene050550.t1 AT3G24120 43.678 174 83 4 204 362 29 202 8.55e-38 139
MS.gene050550.t1 AT4G13640 50.345 145 55 4 216 344 37 180 2.33e-37 138
MS.gene050550.t1 AT1G69580 43.750 160 73 1 205 347 23 182 1.33e-36 137
MS.gene050550.t1 AT3G04030 39.773 176 91 2 204 364 33 208 2.72e-36 137
MS.gene050550.t1 AT1G69580 44.099 161 72 4 205 347 23 183 3.26e-35 133
MS.gene050550.t1 AT5G18240 45.570 158 62 4 212 349 41 194 9.31e-35 134
MS.gene050550.t1 AT5G18240 45.570 158 62 4 212 349 41 194 9.31e-35 134
MS.gene050550.t1 AT1G79430 43.791 153 77 3 201 347 19 168 1.87e-34 132
MS.gene050550.t1 AT5G45580 34.043 235 123 3 216 432 23 243 3.53e-34 129
MS.gene050550.t1 AT5G45580 34.043 235 123 3 216 432 7 227 3.56e-34 129
MS.gene050550.t1 AT3G04030 38.636 176 92 3 204 364 33 207 2.30e-33 129
MS.gene050550.t1 AT5G18240 44.937 158 61 5 212 349 41 192 7.70e-32 125
MS.gene050550.t1 AT3G04030 37.500 176 89 3 204 364 33 202 8.07e-31 122
MS.gene050550.t1 AT2G01060 44.755 143 73 2 251 392 1 138 5.07e-30 117
MS.gene050550.t1 AT5G18240 43.038 158 60 5 212 349 41 188 2.32e-29 119
MS.gene050550.t1 AT5G18240 43.038 158 60 5 212 349 41 188 2.32e-29 119
MS.gene050550.t1 AT3G12730 40.876 137 73 1 216 344 23 159 9.36e-29 113
MS.gene050550.t1 AT5G06800 49.020 102 47 1 185 281 156 257 8.97e-24 100
MS.gene050550.t1 AT4G04605 56.522 69 30 0 217 285 15 83 2.86e-19 86.7
MS.gene050550.t1 AT1G14600 67.857 56 18 0 217 272 24 79 4.15e-19 87.0
MS.gene050550.t1 AT2G42660 59.016 61 25 0 217 277 51 111 7.12e-19 86.3
MS.gene050550.t1 AT5G42630 66.071 56 19 0 217 272 106 161 7.11e-18 82.8
MS.gene050550.t1 AT2G40260 54.321 81 34 2 200 280 67 144 7.13e-18 85.5
MS.gene050550.t1 AT5G42630 66.071 56 19 0 217 272 106 161 1.40e-17 83.2
MS.gene050550.t1 AT2G02060 66.038 53 18 0 217 269 30 82 1.74e-17 82.4
MS.gene050550.t1 AT2G02060 66.038 53 18 0 217 269 30 82 1.74e-17 82.4
MS.gene050550.t1 AT2G38300 60.714 56 22 0 217 272 55 110 4.56e-17 82.4
MS.gene050550.t1 AT1G79430 41.509 106 53 3 248 347 1 103 5.20e-17 81.6
MS.gene050550.t1 AT4G17695 64.286 56 20 0 217 272 165 220 9.52e-17 81.3
MS.gene050550.t1 AT3G04030 35.606 132 70 2 248 364 1 132 1.21e-16 80.9
MS.gene050550.t1 AT1G32240 61.667 60 23 0 213 272 210 269 1.27e-16 81.6
MS.gene050550.t1 AT1G32240 61.667 60 23 0 213 272 210 269 1.57e-16 81.3
MS.gene050550.t1 AT5G16560 61.667 60 23 0 213 272 216 275 1.87e-16 81.3
MS.gene050550.t1 AT2G06020 55.738 61 27 0 212 272 82 142 4.83e-16 79.0
MS.gene050550.t1 AT4G04580 38.776 98 58 1 217 312 15 112 1.15e-15 75.1
MS.gene050550.t1 AT1G25550 56.061 66 29 0 213 278 206 271 6.09e-15 76.3
MS.gene050550.t1 AT2G03500 50.000 76 38 0 207 282 224 299 3.52e-14 74.7
MS.gene050550.t1 AT3G16857 68.519 54 16 1 216 269 167 219 9.72e-14 73.6
MS.gene050550.t1 AT3G16857 68.519 54 16 1 216 269 237 289 1.08e-13 73.6
MS.gene050550.t1 AT3G16857 68.519 54 16 1 216 269 237 289 1.11e-13 73.6
MS.gene050550.t1 AT4G37180 47.500 80 41 1 203 281 197 276 1.50e-13 72.0
MS.gene050550.t1 AT4G37180 47.500 80 41 1 203 281 204 283 1.56e-13 72.0
MS.gene050550.t1 AT3G62670 51.389 72 31 1 216 283 211 282 3.11e-13 71.6
MS.gene050550.t1 AT5G58080 56.667 60 25 1 210 269 188 246 4.04e-13 71.6
MS.gene050550.t1 AT1G68670 56.667 60 26 0 216 275 215 274 8.43e-13 69.7
MS.gene050550.t1 AT5G59570 52.459 61 28 1 209 269 134 193 1.06e-12 68.9
MS.gene050550.t1 AT5G59570 52.459 61 28 1 209 269 134 193 1.06e-12 68.9
MS.gene050550.t1 AT2G25180 59.322 59 23 1 211 269 190 247 1.61e-12 69.7
MS.gene050550.t1 AT5G44190 54.237 59 26 1 214 272 145 202 1.68e-12 69.3
MS.gene050550.t1 AT5G44190 54.237 59 26 1 214 272 145 202 1.82e-12 68.9
MS.gene050550.t1 AT2G40970 42.667 75 42 1 195 269 84 157 5.69e-12 65.9
MS.gene050550.t1 AT2G20570 38.947 95 52 2 213 302 150 243 5.75e-12 67.8
MS.gene050550.t1 AT4G31920 53.226 62 28 1 213 274 180 240 6.23e-12 67.8
MS.gene050550.t1 AT1G49560 55.385 65 29 0 216 280 192 256 6.40e-12 67.0
MS.gene050550.t1 AT3G46640 55.556 54 23 1 216 269 144 196 7.28e-12 66.6
MS.gene050550.t1 AT4G04555 49.180 61 30 1 217 277 15 74 7.31e-12 62.4
MS.gene050550.t1 AT3G46640 55.556 54 23 1 216 269 144 196 7.99e-12 66.6
MS.gene050550.t1 AT3G46640 55.556 54 23 1 216 269 144 196 7.99e-12 66.6
MS.gene050550.t1 AT3G04030 32.576 132 68 3 248 364 1 126 9.93e-12 66.2
MS.gene050550.t1 AT1G67710 53.968 63 29 0 218 280 121 183 1.10e-11 67.0
MS.gene050550.t1 AT2G20570 40.000 90 48 2 218 302 171 259 1.14e-11 66.6
MS.gene050550.t1 AT1G25550 59.259 54 22 0 213 266 206 259 1.14e-11 65.5
MS.gene050550.t1 AT1G67710 53.968 63 29 0 218 280 130 192 1.34e-11 66.6
MS.gene050550.t1 AT5G05090 53.704 54 24 1 216 269 81 133 1.37e-11 65.1
MS.gene050550.t1 AT1G67710 53.968 63 29 0 218 280 195 257 1.41e-11 66.6
MS.gene050550.t1 AT3G12730 32.381 105 63 1 248 344 1 105 2.02e-11 63.2
MS.gene050550.t1 AT3G10760 53.704 54 24 1 216 269 105 157 2.21e-11 65.5
MS.gene050550.t1 AT3G25790 36.090 133 72 3 152 275 127 255 2.38e-11 65.5
MS.gene050550.t1 AT2G01760 43.902 82 43 2 216 295 135 215 3.71e-11 64.3
MS.gene050550.t1 AT2G01760 43.902 82 43 2 216 295 200 280 4.16e-11 64.7
MS.gene050550.t1 AT2G01760 43.902 82 43 2 216 295 228 308 5.88e-11 64.3
MS.gene050550.t1 AT1G49190 46.377 69 34 1 216 281 215 283 6.60e-11 64.3
MS.gene050550.t1 AT4G37180 48.438 64 33 0 203 266 197 260 6.94e-11 63.2
MS.gene050550.t1 AT5G18240 36.441 118 53 5 248 349 1 112 8.75e-11 63.5

Find 94 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTGTTCTTCCCAGTCTTA+TGG 0.207188 7.4:-2234524 MS.gene050550:CDS
CTTTCTCCAGGTATGTCTTT+AGG 0.231387 7.4:+2231847 None:intergenic
CCAAGAAGCTGACTCCAATT+TGG 0.256047 7.4:+2234046 None:intergenic
ATTAGATTCAAGACACCTTT+TGG 0.275565 7.4:+2233376 None:intergenic
ATGATTCTGTTAATCCAAAT+TGG 0.276239 7.4:-2234060 MS.gene050550:CDS
TCTGGACTCCAACGCATTCT+AGG 0.278668 7.4:+2233544 None:intergenic
TGGTGACTCAGGTTTGTATC+TGG 0.307622 7.4:+2233178 None:intergenic
CCAAATTGGAGTCAGCTTCT+TGG 0.308074 7.4:-2234046 MS.gene050550:CDS
GATCCATTGCCAGATATTCT+TGG 0.312892 7.4:-2234202 MS.gene050550:CDS
CCCTTCGCTTCTCAGGCATT+AGG 0.349271 7.4:-2234304 MS.gene050550:CDS
TCAGGAAAACCAAGAATATC+TGG 0.353256 7.4:+2234193 None:intergenic
AGATTCTCCCTTCGCTTCTC+AGG 0.383362 7.4:-2234311 MS.gene050550:CDS
ACAAAAGCTTCGTGAAGTTC+TGG 0.384632 7.4:+2233526 None:intergenic
TGCTGTGAAACGGGAGGAAA+AGG 0.393018 7.4:+2234349 None:intergenic
CTCCCGTTTCACAGCATGAT+AGG 0.396353 7.4:-2234342 MS.gene050550:CDS
GAATTATGGTTACAGGAGTA+AGG 0.405297 7.4:-2232214 MS.gene050550:intron
CAGAAGCAGCATACACCATC+TGG 0.414147 7.4:-2233628 MS.gene050550:CDS
TCCATAAGACTGGGAAGAAC+AGG 0.416578 7.4:+2234523 None:intergenic
AATTGAGGAAATGAAATCTC+TGG 0.418669 7.4:-2232324 MS.gene050550:CDS
ATAATTGCTTTCCATAAGAC+TGG 0.420183 7.4:+2234513 None:intergenic
TGCCTATCATGCTGTGAAAC+GGG 0.423721 7.4:+2234340 None:intergenic
GGAGGAAAAGGGACGCCGCC+AGG 0.438286 7.4:+2234361 None:intergenic
TTTGCTTTCTGCTTACATCA+TGG 0.449248 7.4:+2231821 None:intergenic
AAAGATGAACGGGATAAATC+AGG 0.451170 7.4:-2231886 MS.gene050550:CDS
ATCTACCCTCTCATGTTCAT+TGG 0.454358 7.4:+2231777 None:intergenic
TGTTGTTGAACCAACACAAC+AGG 0.455318 7.4:-2234014 MS.gene050550:intron
TTGCTTTCTGCTTACATCAT+GGG 0.456687 7.4:+2231822 None:intergenic
AGAATATCTGGCAATGGATC+TGG 0.459308 7.4:+2234205 None:intergenic
TGCACCTTCTGCTGCAGTGT+TGG 0.460592 7.4:-2231953 MS.gene050550:CDS
GTTGATGCTGATGCTGAAGC+TGG 0.462318 7.4:+2233583 None:intergenic
GGTGAACGATGAAAAGAATC+CGG 0.468237 7.4:+2234481 None:intergenic
ACATCATGGGAACTTTCTCC+AGG 0.474455 7.4:+2231835 None:intergenic
TGTATACCTTCTGGTGACTC+AGG 0.476063 7.4:+2233167 None:intergenic
TTGCCTATCATGCTGTGAAA+CGG 0.481947 7.4:+2234339 None:intergenic
TCATCTCAAGATGTTTCCCT+TGG 0.484323 7.4:+2232028 None:intergenic
TCAACCAATGATGAATGTCC+TGG 0.485207 7.4:-2234379 MS.gene050550:CDS
CACGACGCCACCTTCTTCTC+CGG 0.487049 7.4:-2231929 MS.gene050550:CDS
ATTAGGTGACAATGTGTCTT+CGG 0.494529 7.4:-2234287 MS.gene050550:CDS
CATGCAGAGGCTACTCCAAA+AGG 0.496159 7.4:-2233391 MS.gene050550:intron
TGATGTAAGCAGAAAGCAAA+TGG 0.496266 7.4:-2231818 MS.gene050550:CDS
TTTGAGAAACAGAAACAGAT+AGG 0.496976 7.4:-2232003 MS.gene050550:CDS
CAGCAGAAGGTGCATTTGAA+GGG 0.498153 7.4:+2231962 None:intergenic
AGGGAATCACTGAAGCATTA+CGG 0.500945 7.4:-2232194 MS.gene050550:CDS
GCCGCCAGGACATTCATCAT+TGG 0.501664 7.4:+2234375 None:intergenic
GCAGCAGAAGGTGCATTTGA+AGG 0.504283 7.4:+2231961 None:intergenic
TTGAATCTAATGAATGTTGA+AGG 0.507075 7.4:-2233364 MS.gene050550:CDS
AAATTGTCCACCGGAGAAGA+AGG 0.507969 7.4:+2231919 None:intergenic
GTGAACGATGAAAAGAATCC+GGG 0.513629 7.4:+2234482 None:intergenic
TCCATATATGAACCTGGAGG+TGG 0.532203 7.4:+2233317 None:intergenic
GAAGGTGCATTTGAAGGGGA+AGG 0.532963 7.4:+2231967 None:intergenic
AAACTAAGCCTAGAATGCGT+TGG 0.536261 7.4:-2233552 MS.gene050550:CDS
ACCTAATGCCTGAGAAGCGA+AGG 0.539904 7.4:+2234303 None:intergenic
TACTCTCACTACCACCAAGC+TGG 0.543524 7.4:+2233491 None:intergenic
AGAAAATGATGATATATCGT+GGG 0.544394 7.4:-2234230 MS.gene050550:CDS
GTGGAAGCAGTCAACCAGCT+TGG 0.551472 7.4:-2233505 MS.gene050550:CDS
GATGTAGCTTGCAATGAGGC+AGG 0.552385 7.4:+2234436 None:intergenic
GAGAAACAAACCTGTTGTGT+TGG 0.559790 7.4:+2234004 None:intergenic
AATTATGGTTACAGGAGTAA+GGG 0.560396 7.4:-2232213 MS.gene050550:intron
AAGAAAATGATGATATATCG+TGG 0.571936 7.4:-2234231 MS.gene050550:CDS
TGAAGCATTACGGTTGCAGA+TGG 0.573866 7.4:-2232184 MS.gene050550:CDS
CAGATTCAAATTGAAAACCA+AGG 0.574230 7.4:-2232045 MS.gene050550:CDS
TTCTGTCCAGCATGATCAGG+TGG 0.575982 7.4:-2234164 MS.gene050550:CDS
TTTGCTGTCAGACCCCTGAC+AGG 0.580082 7.4:+2234460 None:intergenic
AAACCAAGAATATCTGGCAA+TGG 0.585936 7.4:+2234199 None:intergenic
TAATTGTTATACAGATTCAA+AGG 0.586802 7.4:-2232074 MS.gene050550:intron
GACGCCACCTTCTTCTCCGG+TGG 0.587703 7.4:-2231926 MS.gene050550:CDS
TTTCATCGTTCACCTGTCAG+GGG 0.589580 7.4:-2234472 MS.gene050550:CDS
GAAGCAGTCAACCAGCTTGG+TGG 0.594269 7.4:-2233502 MS.gene050550:CDS
GCTGTGAAACGGGAGGAAAA+GGG 0.594959 7.4:+2234350 None:intergenic
AGATTCAAATTGAAAACCAA+GGG 0.608265 7.4:-2232044 MS.gene050550:CDS
AGAACAGGTGAAGACGAAGA+AGG 0.608881 7.4:+2234538 None:intergenic
AGGTGAAGACGAAGAAGGCA+TGG 0.611132 7.4:+2234543 None:intergenic
TAATTGCTTTCCATAAGACT+GGG 0.614268 7.4:+2234514 None:intergenic
GTGTCCAACACTGCAGCAGA+AGG 0.618637 7.4:+2231949 None:intergenic
AAGTTACCAATGAACATGAG+AGG 0.619833 7.4:-2231783 MS.gene050550:CDS
GCGACTTCATGAACAACTTG+AGG 0.620878 7.4:-2232151 MS.gene050550:intron
TCATCATTGGTTGAAAACAC+AGG 0.621388 7.4:+2234388 None:intergenic
AAGCAATTATATGAAACCCC+CGG 0.622265 7.4:-2234500 MS.gene050550:CDS
AAGGTGCATTTGAAGGGGAA+GGG 0.624076 7.4:+2231968 None:intergenic
GACATTCCTAAAGACATACC+TGG 0.630421 7.4:-2231853 MS.gene050550:CDS
AGTTACCAATGAACATGAGA+GGG 0.634728 7.4:-2231782 MS.gene050550:CDS
AGCAGAAGGTGCATTTGAAG+GGG 0.635905 7.4:+2231963 None:intergenic
ACAGGTCGAACACTTCTGAT+CGG 0.639592 7.4:+2234406 None:intergenic
AGATTGTTGACTTCTCCAGA+TGG 0.656610 7.4:+2233613 None:intergenic
CGATGATGTAGCTTGCAATG+AGG 0.659256 7.4:+2234432 None:intergenic
GAAAATGATGATATATCGTG+GGG 0.660394 7.4:-2234229 MS.gene050550:CDS
TACAAACCTGAGTCACCAGA+AGG 0.662240 7.4:-2233173 MS.gene050550:intron
CTATCATGCTGTGAAACGGG+AGG 0.666909 7.4:+2234343 None:intergenic
TTGTCCACCGGAGAAGAAGG+TGG 0.671117 7.4:+2231922 None:intergenic
TGAACGATGAAAAGAATCCG+GGG 0.672098 7.4:+2234483 None:intergenic
CCTAATGCCTGAGAAGCGAA+GGG 0.672541 7.4:+2234304 None:intergenic
ACCAATGATGAATGTCCTGG+CGG 0.684026 7.4:-2234376 MS.gene050550:CDS
GAGAAGTGAATGTTGCTGAG+TGG 0.714972 7.4:+2234260 None:intergenic
GAACGATGAAAAGAATCCGG+GGG 0.737762 7.4:+2234484 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTCACAAAATATTAA+AGG + chr7.4:2233853-2233872 None:intergenic 10.0%
!! TAATAATGTTTACTAATAGT+AGG - chr7.4:2233815-2233834 MS.gene050550:intron 15.0%
!! TACTATTAGTAAACATTATT+AGG + chr7.4:2233816-2233835 None:intergenic 15.0%
!! TCGAATAAATAATAATAGAA+TGG + chr7.4:2232476-2232495 None:intergenic 15.0%
!!! ACTTTTGAAGTTTTAAATAA+AGG + chr7.4:2233425-2233444 None:intergenic 15.0%
!!! GTTTTAAATAAAGGAATAAT+TGG + chr7.4:2233416-2233435 None:intergenic 15.0%
!!! TTATATTTGTTAAAGTGAAA+AGG - chr7.4:2233353-2233372 MS.gene050550:CDS 15.0%
!!! TTCTATTATATTTCTATGTT+TGG + chr7.4:2233562-2233581 None:intergenic 15.0%
!! TAAAAACTTCAAAAGATGAA+CGG - chr7.4:2234373-2234392 MS.gene050550:CDS 20.0%
!! TAATTGTTATACAGATTCAA+AGG - chr7.4:2234196-2234215 MS.gene050550:CDS 20.0%
!! TCTATTATTATTTATTCGAG+AGG - chr7.4:2232477-2232496 MS.gene050550:intron 20.0%
!!! AATATTAAAGGAGTTTTGTT+AGG + chr7.4:2233841-2233860 None:intergenic 20.0%
!!! AATGTCAAAAAAACTGATTT+TGG - chr7.4:2232158-2232177 MS.gene050550:CDS 20.0%
!!! ATATATCATCATTTTCTTGA+AGG + chr7.4:2232037-2232056 None:intergenic 20.0%
!!! ATGTCAAAAAAACTGATTTT+GGG - chr7.4:2232159-2232178 MS.gene050550:CDS 20.0%
!!! CAAAAGACCATTTTAAAAAT+AGG - chr7.4:2233321-2233340 MS.gene050550:intron 20.0%
!!! GATTGTTCCTATTTTTAAAA+TGG + chr7.4:2233331-2233350 None:intergenic 20.0%
! AAAAACTTCAAAAGATGAAC+GGG - chr7.4:2234374-2234393 MS.gene050550:CDS 25.0%
! AAGAAAATGATGATATATCG+TGG - chr7.4:2232039-2232058 MS.gene050550:CDS 25.0%
! AGAAAATGATGATATATCGT+GGG - chr7.4:2232040-2232059 MS.gene050550:CDS 25.0%
! AGATTCAAATTGAAAACCAA+GGG - chr7.4:2234226-2234245 MS.gene050550:CDS 25.0%
! ATAGAATACGATTACACAAT+AGG - chr7.4:2233607-2233626 MS.gene050550:CDS 25.0%
! ATGATTCTGTTAATCCAAAT+TGG - chr7.4:2232210-2232229 MS.gene050550:intron 25.0%
! CACATCCACAAAAAAAAAAT+TGG + chr7.4:2233175-2233194 None:intergenic 25.0%
! CACATTAATGTAATAATTGC+TGG - chr7.4:2233772-2233791 MS.gene050550:intron 25.0%
! CTTCAAATGTAACAAGAATA+AGG + chr7.4:2233497-2233516 None:intergenic 25.0%
! GGATTGATAAAAAGAAGATA+TGG - chr7.4:2233290-2233309 MS.gene050550:intron 25.0%
! GTTAATAGAAATGAAAACCT+AGG + chr7.4:2232439-2232458 None:intergenic 25.0%
! TATAAGAGTAAGAATGTGAA+TGG - chr7.4:2233870-2233889 MS.gene050550:intron 25.0%
! TTGAATCTAATGAATGTTGA+AGG - chr7.4:2232906-2232925 MS.gene050550:intron 25.0%
! TTTGTTAAAGTGAAAAGGAA+AGG - chr7.4:2233358-2233377 MS.gene050550:CDS 25.0%
!! ACATTATTGTTATTATCACC+CGG - chr7.4:2234143-2234162 MS.gene050550:CDS 25.0%
!!! ATTTTTTTTTTGTGGATGTG+TGG - chr7.4:2233175-2233194 MS.gene050550:CDS 25.0%
!!! TCTATTTTTCTCTCATTCAA+AGG - chr7.4:2233448-2233467 MS.gene050550:intron 25.0%
!!! TGCTACCAATTTTTTTTTTG+TGG - chr7.4:2233167-2233186 MS.gene050550:intron 25.0%
!!! TGTCAAAAAAACTGATTTTG+GGG - chr7.4:2232160-2232179 MS.gene050550:CDS 25.0%
AAATGTAACAAGAATAAGGC+GGG + chr7.4:2233493-2233512 None:intergenic 30.0%
AACCTGCTGTATAAAACAAT+AGG + chr7.4:2233146-2233165 None:intergenic 30.0%
AATTATGGTTACAGGAGTAA+GGG - chr7.4:2234057-2234076 MS.gene050550:CDS 30.0%
AATTGAGGAAATGAAATCTC+TGG - chr7.4:2233946-2233965 MS.gene050550:intron 30.0%
ACATCTTTCTCGAAACTATA+AGG + chr7.4:2233211-2233230 None:intergenic 30.0%
ACCATAATTCAAGGAAACAA+TGG + chr7.4:2234046-2234065 None:intergenic 30.0%
ACCTGCTGTATAAAACAATA+GGG + chr7.4:2233145-2233164 None:intergenic 30.0%
AGGCAGTTACTAGATAAATA+GGG + chr7.4:2233718-2233737 None:intergenic 30.0%
ATAATGATGTTAACATGCAG+AGG - chr7.4:2232866-2232885 MS.gene050550:intron 30.0%
ATAATTGCTTTCCATAAGAC+TGG + chr7.4:2231760-2231779 None:intergenic 30.0%
ATGTGCAACAGTTATGTATT+AGG + chr7.4:2233757-2233776 None:intergenic 30.0%
CAAATGTAACAAGAATAAGG+CGG + chr7.4:2233494-2233513 None:intergenic 30.0%
CAGATTCAAATTGAAAACCA+AGG - chr7.4:2234225-2234244 MS.gene050550:CDS 30.0%
CATCTTTCTCGAAACTATAA+GGG + chr7.4:2233210-2233229 None:intergenic 30.0%
GAAAAAGATGAGTTCAATTG+AGG - chr7.4:2233931-2233950 MS.gene050550:intron 30.0%
GAAAATGATGATATATCGTG+GGG - chr7.4:2232041-2232060 MS.gene050550:CDS 30.0%
TAATTGCTTTCCATAAGACT+GGG + chr7.4:2231759-2231778 None:intergenic 30.0%
TAGAACTGATCATCCAAATA+TGG + chr7.4:2232508-2232527 None:intergenic 30.0%
TAGATAAATAGGGAATCTGA+AGG + chr7.4:2233708-2233727 None:intergenic 30.0%
TAGGCAGTTACTAGATAAAT+AGG + chr7.4:2233719-2233738 None:intergenic 30.0%
TCCATTGTTTCCTTGAATTA+TGG - chr7.4:2234042-2234061 MS.gene050550:CDS 30.0%
TTTCCTTGAATTATGGTTAC+AGG - chr7.4:2234049-2234068 MS.gene050550:CDS 30.0%
TTTGAGAAACAGAAACAGAT+AGG - chr7.4:2234267-2234286 MS.gene050550:CDS 30.0%
! ATTAGATTCAAGACACCTTT+TGG + chr7.4:2232897-2232916 None:intergenic 30.0%
! CTTTTTATCAATCCCAACAT+AGG + chr7.4:2233284-2233303 None:intergenic 30.0%
! GAAGTTTTTAAATTGTCCAC+CGG + chr7.4:2234363-2234382 None:intergenic 30.0%
! TATATTTCTATGTTTGGTCC+TGG + chr7.4:2233556-2233575 None:intergenic 30.0%
! TTTTTACATGGTAGATGGTA+AGG + chr7.4:2232932-2232951 None:intergenic 30.0%
AAACCAAGAATATCTGGCAA+TGG + chr7.4:2232074-2232093 None:intergenic 35.0%
AAAGATGAACGGGATAAATC+AGG - chr7.4:2234384-2234403 MS.gene050550:CDS 35.0%
AAATCCATATATGAACCTGG+AGG + chr7.4:2232959-2232978 None:intergenic 35.0%
AAGCAATTATATGAAACCCC+CGG - chr7.4:2231770-2231789 MS.gene050550:CDS 35.0%
AAGTTACCAATGAACATGAG+AGG - chr7.4:2234487-2234506 MS.gene050550:CDS 35.0%
AATGTAACAAGAATAAGGCG+GGG + chr7.4:2233492-2233511 None:intergenic 35.0%
ACAAGCTCAATTTAAGAGGA+CGG + chr7.4:2232983-2233002 None:intergenic 35.0%
ACCAACAAGCTCAATTTAAG+AGG + chr7.4:2232987-2233006 None:intergenic 35.0%
ACTCCTGTAACCATAATTCA+AGG + chr7.4:2234055-2234074 None:intergenic 35.0%
ACTTGAACAAGAACAATCCT+AGG - chr7.4:2232308-2232327 MS.gene050550:intron 35.0%
AGTTACCAATGAACATGAGA+GGG - chr7.4:2234488-2234507 MS.gene050550:CDS 35.0%
ATAAAAATCGACATACACGC+CGG + chr7.4:2233017-2233036 None:intergenic 35.0%
ATACAACCTAAAGGGGAATA+GGG + chr7.4:2232570-2232589 None:intergenic 35.0%
ATTAGGTGACAATGTGTCTT+CGG - chr7.4:2231983-2232002 MS.gene050550:CDS 35.0%
GAATTATGGTTACAGGAGTA+AGG - chr7.4:2234056-2234075 MS.gene050550:CDS 35.0%
GGAAAATCCATATATGAACC+TGG + chr7.4:2232962-2232981 None:intergenic 35.0%
TAGAAATGAAAACCTAGGTG+AGG + chr7.4:2232434-2232453 None:intergenic 35.0%
TAGGAAGCAGTTATGTTTCT+AGG + chr7.4:2233738-2233757 None:intergenic 35.0%
TCAGGAAAACCAAGAATATC+TGG + chr7.4:2232080-2232099 None:intergenic 35.0%
TCCTCTTAAATTGAGCTTGT+TGG - chr7.4:2232983-2233002 MS.gene050550:intron 35.0%
TCGAGAGGAAAATCCATATT+TGG - chr7.4:2232492-2232511 MS.gene050550:intron 35.0%
TCTTCTTCATGTCCTATGTT+GGG - chr7.4:2233269-2233288 MS.gene050550:intron 35.0%
TGATGTAAGCAGAAAGCAAA+TGG - chr7.4:2234452-2234471 MS.gene050550:CDS 35.0%
TGCTGGACAGAAAAAATTTC+AGG + chr7.4:2232098-2232117 None:intergenic 35.0%
TTCTTCTTCATGTCCTATGT+TGG - chr7.4:2233268-2233287 MS.gene050550:intron 35.0%
TTGCTTTCTGCTTACATCAT+GGG + chr7.4:2234451-2234470 None:intergenic 35.0%
TTTGCTTTCTGCTTACATCA+TGG + chr7.4:2234452-2234471 None:intergenic 35.0%
! TCATCATTGGTTGAAAACAC+AGG + chr7.4:2231885-2231904 None:intergenic 35.0%
! TTTTTCTCAGAAGTTTCTGC+AGG + chr7.4:2233917-2233936 None:intergenic 35.0%
!! AAAAAACTGATTTTGGGGAG+TGG - chr7.4:2232165-2232184 MS.gene050550:CDS 35.0%
!! AAAAACTGATTTTGGGGAGT+GGG - chr7.4:2232166-2232185 MS.gene050550:CDS 35.0%
!! ATATCCTACTTTTCTGATGC+AGG - chr7.4:2232605-2232624 MS.gene050550:intron 35.0%
!! TCCCTATTGTTTTATACAGC+AGG - chr7.4:2233141-2233160 MS.gene050550:intron 35.0%
AAACTAAGCCTAGAATGCGT+TGG - chr7.4:2232718-2232737 MS.gene050550:intron 40.0%
AAATGAAAACCTAGGTGAGG+AGG + chr7.4:2232431-2232450 None:intergenic 40.0%
ACAAAAGCTTCGTGAAGTTC+TGG + chr7.4:2232747-2232766 None:intergenic 40.0%
AGAATATCTGGCAATGGATC+TGG + chr7.4:2232068-2232087 None:intergenic 40.0%
AGGTAAGTAAAGTTTGGCCT+AGG + chr7.4:2232328-2232347 None:intergenic 40.0%
ATCTACCCTCTCATGTTCAT+TGG + chr7.4:2234496-2234515 None:intergenic 40.0%
ATGCCAAAACTTCGAAGTTC+CGG + chr7.4:2234165-2234184 None:intergenic 40.0%
ATGTAACAAGAATAAGGCGG+GGG + chr7.4:2233491-2233510 None:intergenic 40.0%
CACAGAAACTTGATGAAACC+AGG - chr7.4:2233535-2233554 MS.gene050550:CDS 40.0%
CATACAACCTAAAGGGGAAT+AGG + chr7.4:2232571-2232590 None:intergenic 40.0%
CTTTCTCCAGGTATGTCTTT+AGG + chr7.4:2234426-2234445 None:intergenic 40.0%
GAAGAAACGTTCATGAGAGA+AGG + chr7.4:2232839-2232858 None:intergenic 40.0%
GACATTCCTAAAGACATACC+TGG - chr7.4:2234417-2234436 MS.gene050550:CDS 40.0%
GAGAAACAAACCTGTTGTGT+TGG + chr7.4:2232269-2232288 None:intergenic 40.0%
GATCCATTGCCAGATATTCT+TGG - chr7.4:2232068-2232087 MS.gene050550:intron 40.0%
GGATTGTTCTTGTTCAAGTC+AGG + chr7.4:2232307-2232326 None:intergenic 40.0%
GGGAATGAACTATATGCACA+TGG + chr7.4:2232550-2232569 None:intergenic 40.0%
GGTGAACGATGAAAAGAATC+CGG + chr7.4:2231792-2231811 None:intergenic 40.0%
GTACACTCTTGTATACCTTC+TGG + chr7.4:2233115-2233134 None:intergenic 40.0%
GTCAAACGAAGGATGAGATT+AGG + chr7.4:2232817-2232836 None:intergenic 40.0%
GTGAACGATGAAAAGAATCC+GGG + chr7.4:2231791-2231810 None:intergenic 40.0%
TCAACCAATGATGAATGTCC+TGG - chr7.4:2231891-2231910 MS.gene050550:CDS 40.0%
TCATCTCAAGATGTTTCCCT+TGG + chr7.4:2234245-2234264 None:intergenic 40.0%
TGAACGATGAAAAGAATCCG+GGG + chr7.4:2231790-2231809 None:intergenic 40.0%
TGTTGTTGAACCAACACAAC+AGG - chr7.4:2232256-2232275 MS.gene050550:intron 40.0%
TTCATTCCCTATTCCCCTTT+AGG - chr7.4:2232561-2232580 MS.gene050550:intron 40.0%
TTGCCTATCATGCTGTGAAA+CGG + chr7.4:2231934-2231953 None:intergenic 40.0%
! AGAACTTCACGAAGCTTTTG+TGG - chr7.4:2232746-2232765 MS.gene050550:intron 40.0%
! AGGGAATCACTGAAGCATTA+CGG - chr7.4:2234076-2234095 MS.gene050550:CDS 40.0%
! GTGGCTTTTTACATGGTAGA+TGG + chr7.4:2232937-2232956 None:intergenic 40.0%
! TGAATCTTCGCTCGTTTTGT+TGG + chr7.4:2234540-2234559 None:intergenic 40.0%
! TTTTCATCGTTCACCTGTCA+GGG - chr7.4:2231797-2231816 MS.gene050550:CDS 40.0%
! TTTTTCTGTCCAGCATGATC+AGG - chr7.4:2232103-2232122 MS.gene050550:intron 40.0%
!! AGATTGTTGACTTCTCCAGA+TGG + chr7.4:2232660-2232679 None:intergenic 40.0%
!! ATTCCCCTTTAGGTTGTATG+CGG - chr7.4:2232571-2232590 MS.gene050550:intron 40.0%
AAATTGTCCACCGGAGAAGA+AGG + chr7.4:2234354-2234373 None:intergenic 45.0%
AACAGCCGCATACAACCTAA+AGG + chr7.4:2232579-2232598 None:intergenic 45.0%
AAGGTGCATTTGAAGGGGAA+GGG + chr7.4:2234305-2234324 None:intergenic 45.0%
ACAGCCGCATACAACCTAAA+GGG + chr7.4:2232578-2232597 None:intergenic 45.0%
ACAGGTCGAACACTTCTGAT+CGG + chr7.4:2231867-2231886 None:intergenic 45.0%
ACATCATGGGAACTTTCTCC+AGG + chr7.4:2234438-2234457 None:intergenic 45.0%
ACCAATGATGAATGTCCTGG+CGG - chr7.4:2231894-2231913 MS.gene050550:CDS 45.0%
ACCTGTTCTTCCCAGTCTTA+TGG - chr7.4:2231746-2231765 MS.gene050550:CDS 45.0%
AGAACAGGTGAAGACGAAGA+AGG + chr7.4:2231735-2231754 None:intergenic 45.0%
ATCGCCTGCATCAGAAAAGT+AGG + chr7.4:2232612-2232631 None:intergenic 45.0%
CATGAGAGAAGGTCAAACGA+AGG + chr7.4:2232828-2232847 None:intergenic 45.0%
CCAAATTGGAGTCAGCTTCT+TGG - chr7.4:2232224-2232243 MS.gene050550:intron 45.0%
CCAAGACTCTCGAATTCCTA+GGG + chr7.4:2233635-2233654 None:intergenic 45.0%
CCTAGGAATTCGAGAGTCTT+GGG - chr7.4:2233633-2233652 MS.gene050550:CDS 45.0%
GAACGATGAAAAGAATCCGG+GGG + chr7.4:2231789-2231808 None:intergenic 45.0%
GAGAAGTGAATGTTGCTGAG+TGG + chr7.4:2232013-2232032 None:intergenic 45.0%
GATTACACAATAGGCTCCCT+AGG - chr7.4:2233616-2233635 MS.gene050550:CDS 45.0%
GCGACTTCATGAACAACTTG+AGG - chr7.4:2234119-2234138 MS.gene050550:CDS 45.0%
GCTGGGAGGTAAGTAAAGTT+TGG + chr7.4:2232334-2232353 None:intergenic 45.0%
TACAAACCTGAGTCACCAGA+AGG - chr7.4:2233097-2233116 MS.gene050550:intron 45.0%
TCCATAAGACTGGGAAGAAC+AGG + chr7.4:2231750-2231769 None:intergenic 45.0%
TCCATATATGAACCTGGAGG+TGG + chr7.4:2232956-2232975 None:intergenic 45.0%
TCTTAAACCTCCAGCTCCTA+AGG + chr7.4:2232379-2232398 None:intergenic 45.0%
TGCCAAAACTTCGAAGTTCC+GGG + chr7.4:2234164-2234183 None:intergenic 45.0%
TGCCTATCATGCTGTGAAAC+GGG + chr7.4:2231933-2231952 None:intergenic 45.0%
TGGTGACTCAGGTTTGTATC+TGG + chr7.4:2233095-2233114 None:intergenic 45.0%
TGTATACCTTCTGGTGACTC+AGG + chr7.4:2233106-2233125 None:intergenic 45.0%
TTTCATCGTTCACCTGTCAG+GGG - chr7.4:2231798-2231817 MS.gene050550:CDS 45.0%
! AGCAGAAGGTGCATTTGAAG+GGG + chr7.4:2234310-2234329 None:intergenic 45.0%
! CAGCAGAAGGTGCATTTGAA+GGG + chr7.4:2234311-2234330 None:intergenic 45.0%
! CCAAGAAGCTGACTCCAATT+TGG + chr7.4:2232227-2232246 None:intergenic 45.0%
! CGATGATGTAGCTTGCAATG+AGG + chr7.4:2231841-2231860 None:intergenic 45.0%
! CTTTTCATCGTTCACCTGTC+AGG - chr7.4:2231796-2231815 MS.gene050550:CDS 45.0%
! TGAAGCATTACGGTTGCAGA+TGG - chr7.4:2234086-2234105 MS.gene050550:CDS 45.0%
! TGTTTTCCACCTGATCATGC+TGG + chr7.4:2232115-2232134 None:intergenic 45.0%
!! ATCTTCGCTCGTTTTGTTGG+AGG + chr7.4:2234537-2234556 None:intergenic 45.0%
ACCTAATGCCTGAGAAGCGA+AGG + chr7.4:2231970-2231989 None:intergenic 50.0%
AGACCAAGCCCTCGAATTCT+TGG + chr7.4:2233668-2233687 None:intergenic 50.0%
AGATTCTCCCTTCGCTTCTC+AGG - chr7.4:2231959-2231978 MS.gene050550:CDS 50.0%
CAAATGGGCCCTAGTGAGTT+CGG + chr7.4:2232356-2232375 None:intergenic 50.0%
CAAGCCCTCGAATTCTTGGA+GGG + chr7.4:2233664-2233683 None:intergenic 50.0%
CAGAAGCAGCATACACCATC+TGG - chr7.4:2232642-2232661 MS.gene050550:intron 50.0%
CAGCCGCATACAACCTAAAG+GGG + chr7.4:2232577-2232596 None:intergenic 50.0%
CATGCAGAGGCTACTCCAAA+AGG - chr7.4:2232879-2232898 MS.gene050550:intron 50.0%
CCATGTAAAAAGCCACCTCC+AGG - chr7.4:2232941-2232960 MS.gene050550:intron 50.0%
CCCAAGACTCTCGAATTCCT+AGG + chr7.4:2233636-2233655 None:intergenic 50.0%
CCCTAGGAATTCGAGAGTCT+TGG - chr7.4:2233632-2233651 MS.gene050550:CDS 50.0%
CCTAATGCCTGAGAAGCGAA+GGG + chr7.4:2231969-2231988 None:intergenic 50.0%
CTATCATGCTGTGAAACGGG+AGG + chr7.4:2231930-2231949 None:intergenic 50.0%
CTCCAGCTCCTAAGGCAAAT+GGG + chr7.4:2232371-2232390 None:intergenic 50.0%
CTCCCGTTTCACAGCATGAT+AGG - chr7.4:2231928-2231947 MS.gene050550:CDS 50.0%
CTCTCCCTCCAAGAATTCGA+GGG - chr7.4:2233657-2233676 MS.gene050550:intron 50.0%
GCCACCTCCAGGTTCATATA+TGG - chr7.4:2232952-2232971 MS.gene050550:intron 50.0%
GCTGTGAAACGGGAGGAAAA+GGG + chr7.4:2231923-2231942 None:intergenic 50.0%
GTTGATGCTGATGCTGAAGC+TGG + chr7.4:2232690-2232709 None:intergenic 50.0%
TACTCTCACTACCACCAAGC+TGG + chr7.4:2232782-2232801 None:intergenic 50.0%
TCTCTCCCTCCAAGAATTCG+AGG - chr7.4:2233656-2233675 MS.gene050550:intron 50.0%
TCTGGACTCCAACGCATTCT+AGG + chr7.4:2232729-2232748 None:intergenic 50.0%
TGCTGTGAAACGGGAGGAAA+AGG + chr7.4:2231924-2231943 None:intergenic 50.0%
TTCTGTCCAGCATGATCAGG+TGG - chr7.4:2232106-2232125 MS.gene050550:intron 50.0%
! CACCCGGAACTTCGAAGTTT+TGG - chr7.4:2234159-2234178 MS.gene050550:CDS 50.0%
! CCTGGAGGTGGCTTTTTACA+TGG + chr7.4:2232944-2232963 None:intergenic 50.0%
! GAAGGTGCATTTGAAGGGGA+AGG + chr7.4:2234306-2234325 None:intergenic 50.0%
! GATGTAGCTTGCAATGAGGC+AGG + chr7.4:2231837-2231856 None:intergenic 50.0%
! GCAGCAGAAGGTGCATTTGA+AGG + chr7.4:2234312-2234331 None:intergenic 50.0%
! TTTTCTGATGCAGGCGATGC+AGG - chr7.4:2232614-2232633 MS.gene050550:intron 50.0%
ATCGACATACACGCCGGTGA+CGG + chr7.4:2233011-2233030 None:intergenic 55.0%
CACTAGGGCCCATTTGCCTT+AGG - chr7.4:2232360-2232379 MS.gene050550:intron 55.0%
CCAAGCCCTCGAATTCTTGG+AGG + chr7.4:2233665-2233684 None:intergenic 55.0%
CCATTTGCCTTAGGAGCTGG+AGG - chr7.4:2232369-2232388 MS.gene050550:intron 55.0%
CCCTTCGCTTCTCAGGCATT+AGG - chr7.4:2231966-2231985 MS.gene050550:CDS 55.0%
CCTCCAAGAATTCGAGGGCT+TGG - chr7.4:2233662-2233681 MS.gene050550:intron 55.0%
CCTCCAGCTCCTAAGGCAAA+TGG + chr7.4:2232372-2232391 None:intergenic 55.0%
GAAGCAGTCAACCAGCTTGG+TGG - chr7.4:2232768-2232787 MS.gene050550:intron 55.0%
GCCGCCAGGACATTCATCAT+TGG + chr7.4:2231898-2231917 None:intergenic 55.0%
GTGGAAGCAGTCAACCAGCT+TGG - chr7.4:2232765-2232784 MS.gene050550:intron 55.0%
GTGTCCAACACTGCAGCAGA+AGG + chr7.4:2234324-2234343 None:intergenic 55.0%
TGCACCTTCTGCTGCAGTGT+TGG - chr7.4:2234317-2234336 MS.gene050550:CDS 55.0%
TTGTCCACCGGAGAAGAAGG+TGG + chr7.4:2234351-2234370 None:intergenic 55.0%
! TTTGCTGTCAGACCCCTGAC+AGG + chr7.4:2231813-2231832 None:intergenic 55.0%
ACACGCAGTCCTCCTCACCT+AGG - chr7.4:2232419-2232438 MS.gene050550:intron 60.0%
ACCTCCCAGCCGAACTCACT+AGG - chr7.4:2232344-2232363 MS.gene050550:CDS 60.0%
CACGACGCCACCTTCTTCTC+CGG - chr7.4:2234341-2234360 MS.gene050550:CDS 60.0%
CCTCCCAGCCGAACTCACTA+GGG - chr7.4:2232345-2232364 MS.gene050550:CDS 60.0%
GGCCCATTTGCCTTAGGAGC+TGG - chr7.4:2232366-2232385 MS.gene050550:intron 60.0%
!! GAGCTTGTTGGTGCCGTCAC+CGG - chr7.4:2232995-2233014 MS.gene050550:intron 60.0%
CCCTAGTGAGTTCGGCTGGG+AGG + chr7.4:2232348-2232367 None:intergenic 65.0%
GACGCCACCTTCTTCTCCGG+TGG - chr7.4:2234344-2234363 MS.gene050550:CDS 65.0%
GGGCCCTAGTGAGTTCGGCT+GGG + chr7.4:2232351-2232370 None:intergenic 65.0%
TGGGCCCTAGTGAGTTCGGC+TGG + chr7.4:2232352-2232371 None:intergenic 65.0%
GGAGGAAAAGGGACGCCGCC+AGG + chr7.4:2231912-2231931 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 2231729 2234563 2231729 ID=MS.gene050550
chr7.4 mRNA 2231729 2234563 2231729 ID=MS.gene050550.t1;Parent=MS.gene050550
chr7.4 exon 2234015 2234563 2234015 ID=MS.gene050550.t1.exon1;Parent=MS.gene050550.t1
chr7.4 CDS 2234015 2234563 2234015 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
chr7.4 exon 2233494 2233665 2233494 ID=MS.gene050550.t1.exon2;Parent=MS.gene050550.t1
chr7.4 CDS 2233494 2233665 2233494 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
chr7.4 exon 2233330 2233406 2233330 ID=MS.gene050550.t1.exon3;Parent=MS.gene050550.t1
chr7.4 CDS 2233330 2233406 2233330 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
chr7.4 exon 2233174 2233213 2233174 ID=MS.gene050550.t1.exon4;Parent=MS.gene050550.t1
chr7.4 CDS 2233174 2233213 2233174 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
chr7.4 exon 2232314 2232371 2232314 ID=MS.gene050550.t1.exon5;Parent=MS.gene050550.t1
chr7.4 CDS 2232314 2232371 2232314 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
chr7.4 exon 2232152 2232221 2232152 ID=MS.gene050550.t1.exon6;Parent=MS.gene050550.t1
chr7.4 CDS 2232152 2232221 2232152 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
chr7.4 exon 2231729 2232082 2231729 ID=MS.gene050550.t1.exon7;Parent=MS.gene050550.t1
chr7.4 CDS 2231729 2232082 2231729 ID=cds.MS.gene050550.t1;Parent=MS.gene050550.t1
Gene Sequence

>MS.gene050550

ATGCCTTCTTCGTCTTCACCTGTTCTTCCCAGTCTTATGGAAAGCAATTATATGAAACCCCCGGATTCTTTTCATCGTTCACCTGTCAGGGGTCTGACAGCAAATCCTGCCTCATTGCAAGCTACATCATCGTATCCGATCAGAAGTGTTCGACCTGTGTTTTCAACCAATGATGAATGTCCTGGCGGCGTCCCTTTTCCTCCCGTTTCACAGCATGATAGGCAATATCAAGATTCTCCCTTCGCTTCTCAGGCATTAGGTGACAATGTGTCTTCGGAAATCCACTCAGCAACATTCACTTCTCACCTTCAAGAAAATGATGATATATCGTGGGGACCAGATCCATTGCCAGATATTCTTGGTTTTCCTGAAATTTTTTCTGTCCAGCATGATCAGGTGGAAAACAGTGCTTGTTACATGAATGAAGACAATGTCAAAAAAACTGATTTTGGGGAGTGGGTTGAACAACTAATGACAAGTGATGATTCTGTTAATCCAAATTGGAGTCAGCTTCTTGGTGATGACAATGTTGTTGAACCAACACAACAGGTTTGTTTCTCAATCTGATTTCTAATCCTGACTTGAACAAGAACAATCCTAGGCCAAACTTTACTTACCTCCCAGCCGAACTCACTAGGGCCCATTTGCCTTAGGAGCTGGAGGTTTAAGACAAAGAAACAAAACAAACACACACGCAGTCCTCCTCACCTAGGTTTTCATTTCTATTAACTTTCTGCATTAATGCCATTCTATTATTATTTATTCGAGAGGAAAATCCATATTTGGATGATCAGTTCTATTATTAATTGAATTTGTCACCATGTGCATATAGTTCATTCCCTATTCCCCTTTAGGTTGTATGCGGCTGTTAACACTATATCCTACTTTTCTGATGCAGGCGATGCAGGTTTCTCAGAAGCAGCATACACCATCTGGAGAAGTCAACAATCTTTGTAATCCAGCTTCAGCATCAGCATCAACTGCTTCTCAAACTAAGCCTAGAATGCGTTGGAGTCCAGAACTTCACGAAGCTTTTGTGGAAGCAGTCAACCAGCTTGGTGGTAGTGAGAGTATGTTTACTTCTTCCTAATCTCATCCTTCGTTTGACCTTCTCTCATGAACGTTTCTTCTCATCATATAATGATGTTAACATGCAGAGGCTACTCCAAAAGGTGTCTTGAATCTAATGAATGTTGAAGGCCTTACCATCTACCATGTAAAAAGCCACCTCCAGGTTCATATATGGATTTTCCGTCCTCTTAAATTGAGCTTGTTGGTGCCGTCACCGGCGTGTATGTCGATTTTTATAATCTAATGTTGTTTATAAATTAATTGTTCTTTTAATTTCAGAAGTATAGAACTGCCAGATACAAACCTGAGTCACCAGAAGGTATACAAGAGTGTACTTATAATCCCTATTGTTTTATACAGCAGGTTTTGCTACCAATTTTTTTTTTGTGGATGTGTGGCTTCTTTATCCCTTATAGTTTCGAGAAAGATGTTTTATTTCAACTTCAAATGTCTTTTTTGTTTGTGTTCTTCTTCTTCATGTCCTATGTTGGGATTGATAAAAAGAAGATATGGATGAAACACAAAAGACCATTTTAAAAATAGGAACAATCTCTTATATTTGTTAAAGTGAAAAGGAAAGGTAGAGAAGAAAGAAAAGTGTAGAAGTAAAAATCCAATTATTCCTTTATTTAAAACTTCAAAAGTTTATCTATTTTTCTCTCATTCAAAGGCAAGTTAGAATGCTTTTCCCCCGCCTTATTCTTGTTACATTTGAAGAATATTTTATTTTATGCACACAGAAACTTGATGAAACCAGGACCAAACATAGAAATATAATAGAAACAATTTAATTTTATTGATAATAGAATAGAATACGATTACACAATAGGCTCCCTAGGAATTCGAGAGTCTTGGGTCTCTCCCTCCAAGAATTCGAGGGCTTGGTCTCTCGCTATTGACATTGTCCTTCAGATTCCCTATTTATCTAGTAACTGCCTAGAAACATAACTGCTTCCTAATACATAACTGTTGCACATTAATGTAATAATTGCTGGCTAATAAAATAATAACTGCCTAATAATGTTTACTAATAGTAGGCCTAACAAAACTCCTTTAATATTTTGTGAAAATATAAGAGTAAGAATGTGAATGGTGAATGAGATCTTCTTCTGTACCTGCAGAAACTTCTGAGAAAAAGATGAGTTCAATTGAGGAAATGAAATCTCTGGACTTGAAAACGTGAGTCATTTCTCTAACTTCTTTTAGCTTTAGATATGATACTTATTGTTCACAAATCACAATTCCATTGTTTCCTTGAATTATGGTTACAGGAGTAAGGGAATCACTGAAGCATTACGGTTGCAGATGGAACTTCAGAAGCGACTTCATGAACAACTTGAGGTACATTATTGTTATTATCACCCGGAACTTCGAAGTTTTGGCATGTCTGATATTGTAATTGTTATACAGATTCAAAGGAAGTTGCAGATTCAAATTGAAAACCAAGGGAAACATCTTGAGATGATGTTTGAGAAACAGAAACAGATAGGTGACAACAAGAGCCCTTCCCCTTCAAATGCACCTTCTGCTGCAGTGTTGGACACGACGCCACCTTCTTCTCCGGTGGACAATTTAAAAACTTCAAAAGATGAACGGGATAAATCAGGATGTAACGCTGACATTCCTAAAGACATACCTGGAGAAAGTTCCCATGATGTAAGCAGAAAGCAAATGGCAGATGAAGCTGAAGTTACCAATGAACATGAGAGGGTAGATGATCAGTTTTCTGACGTGCCTCCAACAAAACGAGCGAAGATTCAATGA
Protein sequence

>MS.gene050550.t1

MPSSSSPVLPSLMESNYMKPPDSFHRSPVRGLTANPASLQATSSYPIRSVRPVFSTNDECPGGVPFPPVSQHDRQYQDSPFASQALGDNVSSEIHSATFTSHLQENDDISWGPDPLPDILGFPEIFSVQHDQVENSACYMNEDNVKKTDFGEWVEQLMTSDDSVNPNWSQLLGDDNVVEPTQQAMQVSQKQHTPSGEVNNLCNPASASASTASQTKPRMRWSPELHEAFVEAVNQLGGSEKATPKGVLNLMNVEGLTIYHVKSHLQKYRTARYKPESPEETSEKKMSSIEEMKSLDLKTSKGITEALRLQMELQKRLHEQLEIQRKLQIQIENQGKHLEMMFEKQKQIGDNKSPSPSNAPSAAVLDTTPPSSPVDNLKTSKDERDKSGCNADIPKDIPGESSHDVSRKQMADEAEVTNEHERVDDQFSDVPPTKRAKIQ