Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051034.t1 | XP_003627433.1 | 85.2 | 183 | 25 | 2 | 1 | 181 | 1 | 183 | 2.40E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051034.t1 | Q9FKK2 | 29.5 | 156 | 105 | 1 | 15 | 165 | 5 | 160 | 5.3e-13 | 75.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051034.t1 | G7L955 | 85.2 | 183 | 25 | 2 | 1 | 181 | 1 | 183 | 1.7e-73 | 285.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051034.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051034.t1 | MTR_8g022970 | 90.710 | 183 | 15 | 2 | 1 | 181 | 1 | 183 | 1.39e-117 | 331 |
| MS.gene051034.t1 | MTR_3g109930 | 73.125 | 160 | 43 | 0 | 22 | 181 | 7 | 166 | 8.60e-80 | 235 |
| MS.gene051034.t1 | MTR_3g437790 | 47.126 | 174 | 85 | 5 | 10 | 179 | 8 | 178 | 1.09e-40 | 136 |
| MS.gene051034.t1 | MTR_2g045020 | 46.067 | 178 | 83 | 5 | 3 | 176 | 8 | 176 | 9.11e-37 | 126 |
| MS.gene051034.t1 | MTR_7g028448 | 37.681 | 138 | 76 | 2 | 35 | 172 | 30 | 157 | 2.14e-23 | 91.3 |
| MS.gene051034.t1 | MTR_4g036915 | 37.681 | 138 | 76 | 2 | 35 | 172 | 30 | 157 | 2.14e-23 | 91.3 |
| MS.gene051034.t1 | MTR_2g105290 | 35.065 | 154 | 98 | 2 | 15 | 168 | 10 | 161 | 1.19e-22 | 89.7 |
| MS.gene051034.t1 | MTR_1g012570 | 33.129 | 163 | 97 | 3 | 10 | 165 | 2 | 159 | 2.30e-19 | 82.4 |
| MS.gene051034.t1 | MTR_8g086290 | 33.117 | 154 | 101 | 2 | 15 | 168 | 10 | 161 | 2.29e-18 | 78.6 |
| MS.gene051034.t1 | MTR_5g053390 | 34.356 | 163 | 95 | 4 | 10 | 165 | 2 | 159 | 8.53e-18 | 78.2 |
| MS.gene051034.t1 | MTR_1g108510 | 30.723 | 166 | 97 | 4 | 10 | 165 | 2 | 159 | 1.61e-17 | 77.4 |
| MS.gene051034.t1 | MTR_1g106070 | 30.120 | 166 | 98 | 3 | 10 | 165 | 2 | 159 | 2.23e-17 | 75.9 |
| MS.gene051034.t1 | MTR_3g030770 | 30.723 | 166 | 97 | 4 | 10 | 165 | 2 | 159 | 2.76e-17 | 76.6 |
| MS.gene051034.t1 | MTR_2g030740 | 31.902 | 163 | 99 | 4 | 10 | 165 | 2 | 159 | 1.12e-16 | 75.1 |
| MS.gene051034.t1 | MTR_1g105910 | 30.818 | 159 | 98 | 3 | 10 | 161 | 2 | 155 | 1.18e-16 | 75.1 |
| MS.gene051034.t1 | MTR_3g052920 | 28.736 | 174 | 106 | 3 | 10 | 173 | 2 | 167 | 3.83e-16 | 73.9 |
| MS.gene051034.t1 | MTR_8g043650 | 30.818 | 159 | 98 | 4 | 10 | 161 | 2 | 155 | 4.21e-16 | 73.6 |
| MS.gene051034.t1 | MTR_2g085280 | 31.847 | 157 | 95 | 3 | 16 | 165 | 8 | 159 | 5.23e-16 | 73.6 |
| MS.gene051034.t1 | MTR_2g085250 | 31.847 | 157 | 95 | 3 | 16 | 165 | 8 | 159 | 5.28e-16 | 73.6 |
| MS.gene051034.t1 | MTR_1g105920 | 28.313 | 166 | 101 | 3 | 10 | 165 | 2 | 159 | 5.80e-16 | 73.2 |
| MS.gene051034.t1 | MTR_5g055100 | 32.099 | 162 | 98 | 3 | 10 | 164 | 2 | 158 | 6.36e-16 | 73.2 |
| MS.gene051034.t1 | MTR_5g045560 | 31.613 | 155 | 94 | 3 | 18 | 165 | 10 | 159 | 1.00e-15 | 72.8 |
| MS.gene051034.t1 | MTR_1g108580 | 30.625 | 160 | 93 | 4 | 16 | 165 | 8 | 159 | 1.44e-15 | 72.0 |
| MS.gene051034.t1 | MTR_4g127140 | 31.724 | 145 | 85 | 3 | 35 | 168 | 30 | 171 | 3.90e-15 | 70.1 |
| MS.gene051034.t1 | MTR_1g105905 | 32.117 | 137 | 81 | 3 | 35 | 164 | 10 | 141 | 6.63e-15 | 70.1 |
| MS.gene051034.t1 | MTR_2g093190 | 30.723 | 166 | 97 | 4 | 10 | 165 | 2 | 159 | 1.14e-14 | 69.7 |
| MS.gene051034.t1 | MTR_1g075600 | 28.743 | 167 | 99 | 4 | 10 | 165 | 2 | 159 | 1.60e-14 | 69.3 |
| MS.gene051034.t1 | MTR_0121s0080 | 33.333 | 168 | 86 | 6 | 13 | 165 | 3 | 159 | 3.46e-14 | 68.6 |
| MS.gene051034.t1 | MTR_3g080940 | 27.329 | 161 | 101 | 3 | 18 | 169 | 10 | 163 | 4.68e-14 | 67.8 |
| MS.gene051034.t1 | MTR_1g063160 | 39.362 | 94 | 56 | 1 | 18 | 110 | 3 | 96 | 1.30e-13 | 65.9 |
| MS.gene051034.t1 | MTR_1g077360 | 29.697 | 165 | 100 | 4 | 10 | 165 | 2 | 159 | 1.58e-13 | 66.6 |
| MS.gene051034.t1 | MTR_8g079502 | 40.278 | 72 | 43 | 0 | 18 | 89 | 3 | 74 | 1.82e-13 | 65.5 |
| MS.gene051034.t1 | MTR_1g047550 | 38.462 | 78 | 48 | 0 | 18 | 95 | 3 | 80 | 1.89e-13 | 65.5 |
| MS.gene051034.t1 | MTR_7g062350 | 32.639 | 144 | 73 | 5 | 35 | 165 | 39 | 171 | 2.05e-13 | 66.6 |
| MS.gene051034.t1 | MTR_4g131030 | 38.961 | 77 | 47 | 0 | 18 | 94 | 3 | 79 | 1.75e-12 | 63.5 |
| MS.gene051034.t1 | MTR_1g054265 | 42.647 | 68 | 39 | 0 | 18 | 85 | 3 | 70 | 1.96e-12 | 62.4 |
| MS.gene051034.t1 | MTR_8g051580 | 40.845 | 71 | 42 | 0 | 18 | 88 | 3 | 73 | 2.60e-12 | 62.4 |
| MS.gene051034.t1 | MTR_3g052870 | 37.179 | 78 | 49 | 0 | 10 | 87 | 2 | 79 | 3.10e-12 | 62.8 |
| MS.gene051034.t1 | MTR_8g046350 | 38.028 | 71 | 44 | 0 | 18 | 88 | 3 | 73 | 3.25e-12 | 62.0 |
| MS.gene051034.t1 | MTR_0121s0100 | 36.842 | 76 | 48 | 0 | 10 | 85 | 2 | 77 | 6.21e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051034.t1 | AT5G60440 | 29.677 | 155 | 104 | 1 | 16 | 165 | 6 | 160 | 6.12e-18 | 79.7 |
| MS.gene051034.t1 | AT2G34440 | 29.940 | 167 | 103 | 4 | 18 | 179 | 3 | 160 | 7.22e-15 | 69.3 |
| MS.gene051034.t1 | AT2G24840 | 29.054 | 148 | 99 | 3 | 21 | 165 | 70 | 214 | 3.28e-14 | 68.9 |
| MS.gene051034.t1 | AT3G66656 | 43.284 | 67 | 38 | 0 | 18 | 84 | 3 | 69 | 6.32e-14 | 66.6 |
| MS.gene051034.t1 | AT1G65360 | 38.824 | 85 | 49 | 1 | 18 | 102 | 8 | 89 | 7.06e-13 | 64.7 |
| MS.gene051034.t1 | AT1G01530 | 30.061 | 163 | 99 | 4 | 15 | 169 | 5 | 160 | 5.46e-12 | 62.8 |
| MS.gene051034.t1 | AT3G04100 | 28.221 | 163 | 103 | 2 | 16 | 168 | 13 | 171 | 1.94e-11 | 60.5 |
Find 19 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTTCATTCTATTGATGATA+TGG | 0.250456 | 8.3:+69000055 | MS.gene051034:CDS |
| TGAGCAATTTAAGACTTCTT+TGG | 0.270286 | 8.3:+69000091 | MS.gene051034:CDS |
| TTAATGACGGTGTTGGGGTT+AGG | 0.312793 | 8.3:-68999852 | None:intergenic |
| GAAGAGAATAATTATTTAGA+AGG | 0.328216 | 8.3:+68999987 | MS.gene051034:CDS |
| TTCTATTGATGATATGGCTT+TGG | 0.335359 | 8.3:+69000061 | MS.gene051034:CDS |
| AAGTGTTTAATGACGGTGTT+GGG | 0.345862 | 8.3:-68999858 | None:intergenic |
| TAATGACGGTGTTGGGGTTA+GGG | 0.379636 | 8.3:-68999851 | None:intergenic |
| CATTAAACACTTTCTCGATA+AGG | 0.404019 | 8.3:+68999869 | MS.gene051034:CDS |
| GCTTAATTTAGATGCATCCT+TGG | 0.469345 | 8.3:+69000124 | MS.gene051034:CDS |
| TTTCCGTCTGAGGAAGCCAT+TGG | 0.485515 | 8.3:-68999627 | None:intergenic |
| AATTTAGATGCATCCTTGGA+AGG | 0.508160 | 8.3:+69000128 | MS.gene051034:CDS |
| GATCCAATGGCTTCCTCAGA+CGG | 0.528355 | 8.3:+68999624 | None:intergenic |
| AAAGTGTTTAATGACGGTGT+TGG | 0.530631 | 8.3:-68999859 | None:intergenic |
| ACCTTCTCAAAACGCAAACT+AGG | 0.541621 | 8.3:+68999732 | MS.gene051034:CDS |
| TGATGTGTTGTTTCCGTCTG+AGG | 0.575813 | 8.3:-68999637 | None:intergenic |
| AACCCTAAAACTTCAACAAG+AGG | 0.577836 | 8.3:+68999944 | MS.gene051034:CDS |
| ATCGAGAAAGTGTTTAATGA+CGG | 0.587110 | 8.3:-68999865 | None:intergenic |
| AACAAGAGGCAATCGAAGAA+CGG | 0.590422 | 8.3:+68999958 | MS.gene051034:CDS |
| AGTGTTTAATGACGGTGTTG+GGG | 0.595656 | 8.3:-68999857 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGAGAATAATTATTTAGA+AGG | + | chr8.3:68999987-69000006 | MS.gene051034:CDS | 20.0% |
| !! | TTCTGCTTTCTTGATTTTAA+TGG | - | chr8.3:68999691-68999710 | None:intergenic | 25.0% |
| AACAAAAAACTCTACGTTTG+TGG | + | chr8.3:68999825-68999844 | MS.gene051034:CDS | 30.0% | |
| CATTAAACACTTTCTCGATA+AGG | + | chr8.3:68999869-68999888 | MS.gene051034:CDS | 30.0% | |
| ! | AGTTTTTGCATTGCATAGAA+TGG | - | chr8.3:68999784-68999803 | None:intergenic | 30.0% |
| ! | GGTTCATTCTATTGATGATA+TGG | + | chr8.3:69000055-69000074 | MS.gene051034:CDS | 30.0% |
| ! | TGAGCAATTTAAGACTTCTT+TGG | + | chr8.3:69000091-69000110 | MS.gene051034:CDS | 30.0% |
| ! | TTCTATTGATGATATGGCTT+TGG | + | chr8.3:69000061-69000080 | MS.gene051034:CDS | 30.0% |
| !! | ATCGAGAAAGTGTTTAATGA+CGG | - | chr8.3:68999868-68999887 | None:intergenic | 30.0% |
| !! | GTAGTTCAAGTTTTTCTTGT+TGG | + | chr8.3:69000030-69000049 | MS.gene051034:CDS | 30.0% |
| !! | TTCAAGTTTTTCTTGTTGGT+GGG | + | chr8.3:69000034-69000053 | MS.gene051034:CDS | 30.0% |
| !!! | CAAACGTAGAGTTTTTTGTT+TGG | - | chr8.3:68999825-68999844 | None:intergenic | 30.0% |
| AACCCTAAAACTTCAACAAG+AGG | + | chr8.3:68999944-68999963 | MS.gene051034:CDS | 35.0% | |
| AAGTGTTTAATGACGGTGTT+GGG | - | chr8.3:68999861-68999880 | None:intergenic | 35.0% | |
| AATTTAGATGCATCCTTGGA+AGG | + | chr8.3:69000128-69000147 | MS.gene051034:CDS | 35.0% | |
| GCTTAATTTAGATGCATCCT+TGG | + | chr8.3:69000124-69000143 | MS.gene051034:CDS | 35.0% | |
| ! | AAAGTGTTTAATGACGGTGT+TGG | - | chr8.3:68999862-68999881 | None:intergenic | 35.0% |
| ! | ATGAATCTTTTTGCCTTCCA+AGG | - | chr8.3:69000144-69000163 | None:intergenic | 35.0% |
| ! | TGCCTCTTGTTGAAGTTTTA+GGG | - | chr8.3:68999949-68999968 | None:intergenic | 35.0% |
| ! | TTGCCTCTTGTTGAAGTTTT+AGG | - | chr8.3:68999950-68999969 | None:intergenic | 35.0% |
| !! | GTTCAAGTTTTTCTTGTTGG+TGG | + | chr8.3:69000033-69000052 | MS.gene051034:CDS | 35.0% |
| AACAAGAGGCAATCGAAGAA+CGG | + | chr8.3:68999958-68999977 | MS.gene051034:CDS | 40.0% | |
| ACCTTCTCAAAACGCAAACT+AGG | + | chr8.3:68999732-68999751 | MS.gene051034:CDS | 40.0% | |
| TAGTCATCGACGCTGAAAAA+AGG | + | chr8.3:68999898-68999917 | MS.gene051034:CDS | 40.0% | |
| ! | AGTGTTTAATGACGGTGTTG+GGG | - | chr8.3:68999860-68999879 | None:intergenic | 40.0% |
| !!! | TCCTAGTTTGCGTTTTGAGA+AGG | - | chr8.3:68999736-68999755 | None:intergenic | 40.0% |
| !!! | TGCGTTTTGAGAAGGTATCA+CGG | - | chr8.3:68999728-68999747 | None:intergenic | 40.0% |
| !!! | TTTTTTCAGCGTCGATGACT+AGG | - | chr8.3:68999899-68999918 | None:intergenic | 40.0% |
| ! | TAATGACGGTGTTGGGGTTA+GGG | - | chr8.3:68999854-68999873 | None:intergenic | 45.0% |
| ! | TTAATGACGGTGTTGGGGTT+AGG | - | chr8.3:68999855-68999874 | None:intergenic | 45.0% |
| !! | TGATGTGTTGTTTCCGTCTG+AGG | - | chr8.3:68999640-68999659 | None:intergenic | 45.0% |
| !!! | CGTTTTGAGAAGGTATCACG+GGG | - | chr8.3:68999726-68999745 | None:intergenic | 45.0% |
| !!! | GCGTTTTGAGAAGGTATCAC+GGG | - | chr8.3:68999727-68999746 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 68999630 | 69000175 | 68999630 | ID=MS.gene051034 |
| chr8.3 | mRNA | 68999630 | 69000175 | 68999630 | ID=MS.gene051034.t1;Parent=MS.gene051034 |
| chr8.3 | exon | 68999630 | 69000175 | 68999630 | ID=MS.gene051034.t1.exon1;Parent=MS.gene051034.t1 |
| chr8.3 | CDS | 68999630 | 69000175 | 68999630 | ID=cds.MS.gene051034.t1;Parent=MS.gene051034.t1 |
| Gene Sequence |
| Protein sequence |
