AlfalfaGEDB | MS.gene051276

M. sativa cultivar XinJiangDaYe / MS.gene051276

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051276.t1	AES77546.1	89.9	159	16	0	1	159	1	159	1.80E-72	282

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051276.t1	Q9FJK3	44.2	156	86	1	1	155	1	156	5.1e-28	125.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051276.t1	G7L356	89.9	159	16	0	1	159	1	159	1.3e-72	282.0

TFs/TRs:

Gene ID	Type	Classification
MS.gene051276.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051276.t1	MTR_7g011950	89.937	159	16	0	1	159	1	159	6.63e-98	279
MS.gene051276.t1	MTR_4g028720	89.308	159	17	0	1	159	1	159	1.30e-95	273
MS.gene051276.t1	MTR_1g077320	83.019	159	27	0	1	159	1	159	4.80e-89	256
MS.gene051276.t1	MTR_1g077390	68.790	157	49	0	1	157	1	157	3.81e-75	221
MS.gene051276.t1	MTR_5g047580	68.590	156	49	0	1	156	1	156	6.81e-75	221
MS.gene051276.t1	MTR_1g084950	73.718	156	41	0	1	156	1	156	4.95e-74	219
MS.gene051276.t1	MTR_1g077300	72.956	159	41	1	1	159	1	157	2.41e-71	212
MS.gene051276.t1	MTR_5g047560	58.974	156	48	1	1	156	1	140	1.82e-57	176
MS.gene051276.t1	MTR_1g090783	54.902	153	69	0	3	155	2	154	1.58e-56	174
MS.gene051276.t1	MTR_1g090697	52.258	155	74	0	1	155	1	155	8.04e-56	172
MS.gene051276.t1	MTR_1g090710	51.282	156	75	1	1	155	1	156	7.67e-54	167
MS.gene051276.t1	MTR_3g466980	43.791	153	85	1	1	152	1	153	5.12e-39	132
MS.gene051276.t1	MTR_4g032620	39.355	155	92	2	1	153	1	155	1.57e-38	131
MS.gene051276.t1	MTR_3g065100	45.860	157	84	1	1	156	1	157	2.39e-36	125
MS.gene051276.t1	MTR_5g075380	35.897	156	99	1	1	155	1	156	1.86e-32	113
MS.gene051276.t1	MTR_4g063790	36.478	159	99	2	1	157	1	159	1.88e-32	114
MS.gene051276.t1	MTR_8g036130	38.994	159	93	2	1	155	1	159	1.94e-32	115
MS.gene051276.t1	MTR_3g466830	38.854	157	95	1	3	158	2	158	2.59e-30	108
MS.gene051276.t1	MTR_4g031910	39.333	150	89	2	1	148	1	150	5.87e-28	103
MS.gene051276.t1	MTR_3g466890	37.662	154	95	1	3	155	2	155	1.67e-27	100
MS.gene051276.t1	MTR_3g031240	34.591	159	102	2	1	157	1	159	3.57e-27	100
MS.gene051276.t1	MTR_3g467080	33.333	159	104	2	1	157	1	159	3.67e-27	100
MS.gene051276.t1	MTR_4g032260	38.750	160	96	2	1	158	1	160	4.48e-27	99.8
MS.gene051276.t1	MTR_2g016210	36.478	159	99	2	1	157	1	159	1.72e-26	100
MS.gene051276.t1	MTR_4g032290	36.646	161	100	2	1	159	1	161	1.02e-24	95.5
MS.gene051276.t1	MTR_2g035610	40.678	118	69	1	1	117	1	118	2.31e-24	91.7
MS.gene051276.t1	MTR_3g031100	34.810	158	99	3	1	155	1	157	2.87e-24	94.7
MS.gene051276.t1	MTR_2g035580	34.194	155	101	1	1	154	1	155	4.37e-24	92.0
MS.gene051276.t1	MTR_4g028800	47.945	73	36	1	1	73	1	71	1.16e-20	80.9
MS.gene051276.t1	MTR_3g465410	33.588	131	85	2	29	157	1	131	1.22e-19	80.1
MS.gene051276.t1	MTR_3g466900	36.800	125	78	1	32	155	2	126	1.47e-19	79.7
MS.gene051276.t1	MTR_3g466930	34.400	125	81	1	32	155	2	126	1.63e-17	74.3
MS.gene051276.t1	MTR_4g019670	33.333	132	84	2	1	128	1	132	4.34e-16	74.7
MS.gene051276.t1	MTR_7g106510	33.884	121	68	3	3	111	6	126	2.93e-15	72.4
MS.gene051276.t1	MTR_1g033370	45.745	94	50	1	64	156	28	121	1.52e-14	66.2
MS.gene051276.t1	MTR_1g033930	41.837	98	56	1	62	158	20	117	2.30e-14	65.9
MS.gene051276.t1	MTR_1g033790	38.938	113	62	2	53	158	5	117	2.52e-13	63.2
MS.gene051276.t1	MTR_7g055800	32.174	115	51	2	1	115	1	88	5.97e-13	61.2
MS.gene051276.t1	MTR_7g055790	35.780	109	53	2	1	109	59	150	9.29e-12	59.7
MS.gene051276.t1	MTR_6g005450	31.250	128	75	5	1	115	1	128	4.03e-11	60.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene051276.t1	AT5G48670	44.444	153	84	1	1	152	1	153	3.20e-32	117
MS.gene051276.t1	AT3G05860	38.255	149	91	1	1	148	1	149	6.76e-31	110
MS.gene051276.t1	AT3G05860	38.255	149	91	1	1	148	1	149	4.03e-30	110
MS.gene051276.t1	AT3G05860	38.255	149	91	1	1	148	1	149	5.18e-30	110
MS.gene051276.t1	AT5G26630	37.500	160	99	1	1	159	1	160	1.46e-27	102
MS.gene051276.t1	AT1G65300	35.211	142	91	1	3	143	2	143	8.31e-27	102
MS.gene051276.t1	AT1G65330	34.694	147	95	1	3	148	2	148	1.35e-24	96.3
MS.gene051276.t1	AT5G26650	33.333	153	101	1	4	155	2	154	9.65e-24	95.5
MS.gene051276.t1	AT5G27960	32.680	153	102	1	4	155	2	154	2.18e-22	91.3
MS.gene051276.t1	AT2G28700	34.211	152	93	2	1	148	1	149	4.29e-22	90.5
MS.gene051276.t1	AT1G31630	29.032	155	108	2	3	156	2	155	5.66e-21	87.8
MS.gene051276.t1	AT5G27810	41.667	96	55	1	29	123	1	96	9.19e-20	79.7
MS.gene051276.t1	AT1G22590	28.395	162	100	3	1	155	1	153	1.14e-19	80.9
MS.gene051276.t1	AT1G31640	31.333	150	101	2	3	151	2	150	2.60e-18	80.9
MS.gene051276.t1	AT5G26580	36.752	117	61	1	1	117	1	104	2.36e-17	77.8
MS.gene051276.t1	AT2G40210	37.815	119	69	2	1	116	1	117	1.11e-14	70.5
MS.gene051276.t1	AT5G06500	29.560	159	108	3	1	157	1	157	1.92e-13	65.9
MS.gene051276.t1	AT5G58890	30.952	126	73	4	1	113	1	125	1.32e-11	61.2

Find 27 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTTAAATCATTGGAAATAA+AGG	0.249979	7.3:-50908240	None:intergenic
AGCTGAACATTCTTATGTTT+TGG	0.259719	7.3:+50908546	MS.gene051276:CDS
CTCCTTTCTTGTTAAATCAT+TGG	0.274579	7.3:-50908249	None:intergenic
TTTGCAAAAGGAAACTTTCT+TGG	0.282244	7.3:-50908466	None:intergenic
TGTTTGGCTTCCTTTAGATT+TGG	0.353502	7.3:-50908389	None:intergenic
ATACCTCTCAATCACCTGTT+TGG	0.354870	7.3:-50908405	None:intergenic
TTCCAATGATTTAACAAGAA+AGG	0.369739	7.3:+50908247	MS.gene051276:CDS
AAATCTAAAGGAAGCCAAAC+AGG	0.425294	7.3:+50908391	MS.gene051276:CDS
CTTTAGTAATCATTTGCAAA+AGG	0.447168	7.3:-50908478	None:intergenic
TCAGAAACAGTCACATCATC+TGG	0.447805	7.3:-50908590	None:intergenic
TGTTTCTGATTTGAAAGACT+TGG	0.473410	7.3:+50908604	MS.gene051276:CDS
TGCAAATGATTACTAAAGCT+AGG	0.481563	7.3:+50908483	MS.gene051276:CDS
TTTCACCTTCTTCCTAGTCA+TGG	0.490416	7.3:-50908213	None:intergenic
GCAAATGATTACTAAAGCTA+GGG	0.492435	7.3:+50908484	MS.gene051276:CDS
AGATGTGTGGCCAAATCTAA+AGG	0.509466	7.3:+50908379	MS.gene051276:CDS
AGAGAACTCACTATTCTTTG+TGG	0.526508	7.3:+50908311	MS.gene051276:CDS
ACATCTGTTTCAGAATCAAA+AGG	0.540441	7.3:-50908362	None:intergenic
CAAAGCCATGACTAGGAAGA+AGG	0.543154	7.3:+50908208	None:intergenic
GAAGGAAATTGATGACAAGA+TGG	0.549956	7.3:+50908649	MS.gene051276:CDS
CAAGAAAGGAGACCTACAAA+AGG	0.555324	7.3:+50908261	MS.gene051276:CDS
AGGAGACCTACAAAAGGAGG+AGG	0.574164	7.3:+50908267	MS.gene051276:CDS
GAGGAAAGGTATCGTCAAGA+AGG	0.592179	7.3:+50908286	MS.gene051276:CDS
GAAAGGAGACCTACAAAAGG+AGG	0.592705	7.3:+50908264	MS.gene051276:CDS
ACCTACAAAAGGAGGAGGAA+AGG	0.609717	7.3:+50908272	MS.gene051276:CDS
TACATTGCAAAGCCATGACT+AGG	0.609749	7.3:+50908201	None:intergenic
AAGCCAAACAGGTGATTGAG+AGG	0.637552	7.3:+50908402	MS.gene051276:CDS
TTGATTCTGAAACAGATGTG+TGG	0.682185	7.3:+50908366	MS.gene051276:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTAATTGAAAAAAATATGA+AGG	+	chr7.3:50908631-50908650	MS.gene051276:CDS	15.0%
!!	TGTTAAATCATTGGAAATAA+AGG	-	chr7.3:50908243-50908262	None:intergenic	20.0%
!	CTTTAGTAATCATTTGCAAA+AGG	-	chr7.3:50908481-50908500	None:intergenic	25.0%
!	TTCCAATGATTTAACAAGAA+AGG	+	chr7.3:50908247-50908266	MS.gene051276:CDS	25.0%
	ACATCTGTTTCAGAATCAAA+AGG	-	chr7.3:50908365-50908384	None:intergenic	30.0%
	CTCCTTTCTTGTTAAATCAT+TGG	-	chr7.3:50908252-50908271	None:intergenic	30.0%
	GCAAATGATTACTAAAGCTA+GGG	+	chr7.3:50908484-50908503	MS.gene051276:CDS	30.0%
	TGCAAATGATTACTAAAGCT+AGG	+	chr7.3:50908483-50908502	MS.gene051276:CDS	30.0%
	TTTGCAAAAGGAAACTTTCT+TGG	-	chr7.3:50908469-50908488	None:intergenic	30.0%
!	AGCTGAACATTCTTATGTTT+TGG	+	chr7.3:50908546-50908565	MS.gene051276:CDS	30.0%
!	TGTTTCTGATTTGAAAGACT+TGG	+	chr7.3:50908604-50908623	MS.gene051276:CDS	30.0%
	AAATCTAAAGGAAGCCAAAC+AGG	+	chr7.3:50908391-50908410	MS.gene051276:CDS	35.0%
	AGAGAACTCACTATTCTTTG+TGG	+	chr7.3:50908311-50908330	MS.gene051276:CDS	35.0%
!	GAAGGAAATTGATGACAAGA+TGG	+	chr7.3:50908649-50908668	MS.gene051276:CDS	35.0%
!	TGTTTGGCTTCCTTTAGATT+TGG	-	chr7.3:50908392-50908411	None:intergenic	35.0%
!!	TTGATTCTGAAACAGATGTG+TGG	+	chr7.3:50908366-50908385	MS.gene051276:CDS	35.0%
	AGATGTGTGGCCAAATCTAA+AGG	+	chr7.3:50908379-50908398	MS.gene051276:CDS	40.0%
	ATACCTCTCAATCACCTGTT+TGG	-	chr7.3:50908408-50908427	None:intergenic	40.0%
	CAAGAAAGGAGACCTACAAA+AGG	+	chr7.3:50908261-50908280	MS.gene051276:CDS	40.0%
	TCAGAAACAGTCACATCATC+TGG	-	chr7.3:50908593-50908612	None:intergenic	40.0%
	AAGCCAAACAGGTGATTGAG+AGG	+	chr7.3:50908402-50908421	MS.gene051276:CDS	45.0%
	ACCTACAAAAGGAGGAGGAA+AGG	+	chr7.3:50908272-50908291	MS.gene051276:CDS	45.0%
	GAAAGGAGACCTACAAAAGG+AGG	+	chr7.3:50908264-50908283	MS.gene051276:CDS	45.0%
!	ACCTTTCCTCCTCCTTTTGT+AGG	-	chr7.3:50908276-50908295	None:intergenic	45.0%
!!	GAGGAAAGGTATCGTCAAGA+AGG	+	chr7.3:50908286-50908305	MS.gene051276:CDS	45.0%
	AGGAGACCTACAAAAGGAGG+AGG	+	chr7.3:50908267-50908286	MS.gene051276:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	50908215	50908694	50908215	ID=MS.gene051276
chr7.3	mRNA	50908215	50908694	50908215	ID=MS.gene051276.t1;Parent=MS.gene051276
chr7.3	exon	50908215	50908694	50908215	ID=MS.gene051276.t1.exon1;Parent=MS.gene051276.t1
chr7.3	CDS	50908215	50908694	50908215	ID=cds.MS.gene051276.t1;Parent=MS.gene051276.t1

Gene Sequence

>MS.gene051276

ATGACTAGGAAGAAGGTGAAACTCGCCTTTATTTCCAATGATTTAACAAGAAAGGAGACCTACAAAAGGAGGAGGAAAGGTATCGTCAAGAAGGTGAGAGAACTCACTATTCTTTGTGGTATTTCAGCATGTGCTATAATTTCTGATCCTTTTGATTCTGAAACAGATGTGTGGCCAAATCTAAAGGAAGCCAAACAGGTGATTGAGAGGTATCAAAACTCATATATGAAAGATGAGAGAGAAAATGTGAACCAAGAAAGTTTCCTTTTGCAAATGATTACTAAAGCTAGGGAACAACTGAGAATGCAAAGATACGACAATCATGAGAATGAGCTGAACATTCTTATGTTTTGGTACATGCAAAACAACAAGCTGCCAGATGATGTGACTGTTTCTGATTTGAAAGACTTGGATAAGTTAATTGAAAAAAATATGAAGGAAATTGATGACAAGATGGCTTCACTTAGTTTATCAAACTGA

Protein sequence

>MS.gene051276.t1

MTRKKVKLAFISNDLTRKETYKRRRKGIVKKVRELTILCGISACAIISDPFDSETDVWPNLKEAKQVIERYQNSYMKDERENVNQESFLLQMITKAREQLRMQRYDNHENELNILMFWYMQNNKLPDDVTVSDLKDLDKLIEKNMKEIDDKMASLSLSN