Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051280.t1 | XP_013468829.1 | 92.5 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 8.10E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051280.t1 | Q9FJK3 | 43.5 | 161 | 90 | 1 | 1 | 160 | 1 | 161 | 1.3e-26 | 120.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051280.t1 | A0A072VMQ1 | 92.5 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 5.9e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051280.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051280.t1 | MTR_1g077320 | 92.453 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 1.00e-97 | 279 |
| MS.gene051280.t1 | MTR_7g011950 | 88.679 | 159 | 18 | 0 | 1 | 159 | 1 | 159 | 2.14e-93 | 268 |
| MS.gene051280.t1 | MTR_4g028720 | 86.792 | 159 | 21 | 0 | 1 | 159 | 1 | 159 | 2.08e-92 | 265 |
| MS.gene051280.t1 | MTR_1g077390 | 70.064 | 157 | 47 | 0 | 1 | 157 | 1 | 157 | 6.15e-77 | 226 |
| MS.gene051280.t1 | MTR_5g047580 | 69.231 | 156 | 48 | 0 | 1 | 156 | 1 | 156 | 1.06e-74 | 220 |
| MS.gene051280.t1 | MTR_1g077300 | 74.214 | 159 | 39 | 1 | 1 | 159 | 1 | 157 | 3.42e-74 | 219 |
| MS.gene051280.t1 | MTR_1g084950 | 73.077 | 156 | 42 | 0 | 1 | 156 | 1 | 156 | 1.05e-72 | 215 |
| MS.gene051280.t1 | MTR_1g090697 | 56.774 | 155 | 67 | 0 | 1 | 155 | 1 | 155 | 1.11e-58 | 180 |
| MS.gene051280.t1 | MTR_1g090783 | 55.556 | 153 | 68 | 0 | 3 | 155 | 2 | 154 | 2.12e-56 | 174 |
| MS.gene051280.t1 | MTR_5g047560 | 58.333 | 156 | 49 | 1 | 1 | 156 | 1 | 140 | 2.74e-56 | 173 |
| MS.gene051280.t1 | MTR_1g090710 | 55.128 | 156 | 69 | 1 | 1 | 155 | 1 | 156 | 3.31e-56 | 174 |
| MS.gene051280.t1 | MTR_5g075380 | 38.462 | 156 | 95 | 1 | 1 | 155 | 1 | 156 | 1.75e-37 | 126 |
| MS.gene051280.t1 | MTR_3g065100 | 48.408 | 157 | 80 | 1 | 1 | 156 | 1 | 157 | 8.52e-37 | 127 |
| MS.gene051280.t1 | MTR_3g466980 | 42.484 | 153 | 87 | 1 | 1 | 152 | 1 | 153 | 2.76e-35 | 122 |
| MS.gene051280.t1 | MTR_4g032620 | 37.419 | 155 | 95 | 2 | 1 | 153 | 1 | 155 | 1.47e-34 | 121 |
| MS.gene051280.t1 | MTR_4g063790 | 38.365 | 159 | 96 | 2 | 1 | 157 | 1 | 159 | 4.78e-33 | 115 |
| MS.gene051280.t1 | MTR_3g466830 | 41.401 | 157 | 91 | 1 | 3 | 158 | 2 | 158 | 3.68e-32 | 112 |
| MS.gene051280.t1 | MTR_4g031910 | 44.000 | 150 | 82 | 2 | 1 | 148 | 1 | 150 | 9.81e-32 | 114 |
| MS.gene051280.t1 | MTR_3g466890 | 39.623 | 159 | 95 | 1 | 3 | 160 | 2 | 160 | 1.19e-29 | 106 |
| MS.gene051280.t1 | MTR_2g016210 | 39.623 | 159 | 94 | 2 | 1 | 157 | 1 | 159 | 6.40e-29 | 107 |
| MS.gene051280.t1 | MTR_8g036130 | 37.736 | 159 | 95 | 2 | 1 | 155 | 1 | 159 | 1.33e-28 | 106 |
| MS.gene051280.t1 | MTR_4g032260 | 41.875 | 160 | 91 | 2 | 1 | 158 | 1 | 160 | 2.65e-28 | 103 |
| MS.gene051280.t1 | MTR_3g031240 | 33.962 | 159 | 103 | 2 | 1 | 157 | 1 | 159 | 6.73e-28 | 102 |
| MS.gene051280.t1 | MTR_4g032290 | 40.994 | 161 | 93 | 2 | 1 | 159 | 1 | 161 | 7.19e-27 | 101 |
| MS.gene051280.t1 | MTR_3g467080 | 32.919 | 161 | 106 | 2 | 1 | 159 | 1 | 161 | 2.64e-26 | 97.8 |
| MS.gene051280.t1 | MTR_3g031100 | 34.810 | 158 | 99 | 3 | 1 | 155 | 1 | 157 | 3.44e-25 | 97.4 |
| MS.gene051280.t1 | MTR_2g035610 | 41.880 | 117 | 67 | 1 | 1 | 116 | 1 | 117 | 7.73e-24 | 90.5 |
| MS.gene051280.t1 | MTR_2g035580 | 32.258 | 155 | 104 | 1 | 1 | 154 | 1 | 155 | 1.52e-21 | 85.5 |
| MS.gene051280.t1 | MTR_3g465410 | 35.115 | 131 | 83 | 2 | 29 | 157 | 1 | 131 | 3.80e-21 | 84.3 |
| MS.gene051280.t1 | MTR_3g466900 | 36.923 | 130 | 81 | 1 | 32 | 160 | 2 | 131 | 1.86e-19 | 79.7 |
| MS.gene051280.t1 | MTR_3g466930 | 36.000 | 125 | 79 | 1 | 32 | 155 | 2 | 126 | 4.48e-18 | 75.9 |
| MS.gene051280.t1 | MTR_4g028800 | 45.205 | 73 | 38 | 1 | 1 | 73 | 1 | 71 | 6.86e-18 | 73.9 |
| MS.gene051280.t1 | MTR_4g019670 | 32.237 | 152 | 96 | 3 | 1 | 148 | 1 | 149 | 7.91e-18 | 79.7 |
| MS.gene051280.t1 | MTR_7g106510 | 35.398 | 113 | 69 | 2 | 1 | 109 | 1 | 113 | 1.55e-16 | 76.3 |
| MS.gene051280.t1 | MTR_7g055800 | 33.333 | 114 | 49 | 2 | 1 | 114 | 1 | 87 | 7.55e-14 | 63.9 |
| MS.gene051280.t1 | MTR_7g055790 | 38.532 | 109 | 50 | 2 | 1 | 109 | 59 | 150 | 2.68e-13 | 63.9 |
| MS.gene051280.t1 | MTR_1g033790 | 39.583 | 96 | 57 | 1 | 64 | 158 | 22 | 117 | 6.59e-12 | 59.7 |
| MS.gene051280.t1 | MTR_1g033930 | 37.500 | 96 | 59 | 1 | 64 | 158 | 22 | 117 | 9.85e-12 | 59.3 |
| MS.gene051280.t1 | MTR_1g033370 | 40.426 | 94 | 55 | 1 | 64 | 156 | 28 | 121 | 1.02e-11 | 58.9 |
| MS.gene051280.t1 | MTR_7g055940 | 34.286 | 105 | 42 | 3 | 1 | 105 | 1 | 78 | 1.37e-11 | 57.8 |
| MS.gene051280.t1 | MTR_3g093900 | 26.316 | 152 | 107 | 2 | 3 | 151 | 6 | 155 | 1.68e-11 | 61.6 |
| MS.gene051280.t1 | MTR_6g005450 | 30.357 | 112 | 72 | 3 | 1 | 106 | 1 | 112 | 8.78e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051280.t1 | AT5G48670 | 44.231 | 156 | 86 | 1 | 1 | 155 | 1 | 156 | 8.29e-32 | 116 |
| MS.gene051280.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 7.41e-31 | 110 |
| MS.gene051280.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 3.31e-30 | 110 |
| MS.gene051280.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 5.81e-30 | 110 |
| MS.gene051280.t1 | AT5G26630 | 38.750 | 160 | 97 | 1 | 1 | 159 | 1 | 160 | 1.85e-28 | 105 |
| MS.gene051280.t1 | AT1G65300 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 3.55e-27 | 103 |
| MS.gene051280.t1 | AT1G65330 | 36.735 | 147 | 92 | 1 | 3 | 148 | 2 | 148 | 9.55e-27 | 102 |
| MS.gene051280.t1 | AT5G26650 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 2.01e-23 | 94.7 |
| MS.gene051280.t1 | AT5G27960 | 42.991 | 107 | 60 | 1 | 4 | 109 | 2 | 108 | 1.62e-22 | 91.7 |
| MS.gene051280.t1 | AT1G22590 | 29.630 | 162 | 98 | 3 | 1 | 155 | 1 | 153 | 1.42e-20 | 83.2 |
| MS.gene051280.t1 | AT2G28700 | 35.526 | 152 | 91 | 2 | 1 | 148 | 1 | 149 | 5.12e-20 | 85.1 |
| MS.gene051280.t1 | AT1G31630 | 29.878 | 164 | 110 | 3 | 3 | 162 | 2 | 164 | 1.64e-19 | 84.0 |
| MS.gene051280.t1 | AT5G27810 | 41.860 | 86 | 49 | 1 | 29 | 113 | 1 | 86 | 2.98e-18 | 76.3 |
| MS.gene051280.t1 | AT1G31640 | 38.739 | 111 | 66 | 2 | 3 | 112 | 2 | 111 | 8.14e-18 | 79.7 |
| MS.gene051280.t1 | AT5G26580 | 38.532 | 109 | 54 | 1 | 1 | 109 | 1 | 96 | 7.15e-17 | 76.6 |
| MS.gene051280.t1 | AT5G58890 | 36.036 | 111 | 63 | 3 | 1 | 104 | 1 | 110 | 2.69e-13 | 66.2 |
| MS.gene051280.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 2.91e-13 | 66.6 |
| MS.gene051280.t1 | AT5G06500 | 39.091 | 110 | 55 | 5 | 1 | 102 | 1 | 106 | 6.14e-12 | 62.0 |
Find 25 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCAAAAGGAAACTTTCC+TGG | 0.331570 | 7.3:-50829443 | None:intergenic |
| AACAGAGGTGTGGCCAAATC+TGG | 0.333286 | 7.3:+50829353 | MS.gene051280:CDS |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 7.3:-50829217 | None:intergenic |
| CTTGTCTTTGCTTTCTCAGT+TGG | 0.347479 | 7.3:-50829485 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.353862 | 7.3:-50829226 | None:intergenic |
| CTCCAATGATTCAACAAGAA+AGG | 0.407412 | 7.3:+50829224 | MS.gene051280:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 7.3:-50829455 | None:intergenic |
| TGTTTCTGATTTGAAAGACT+TGG | 0.473410 | 7.3:+50829581 | MS.gene051280:CDS |
| TCAGAAACAGTCATATCATC+TGG | 0.489973 | 7.3:-50829567 | None:intergenic |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7.3:-50829190 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.499930 | 7.3:+50829288 | MS.gene051280:CDS |
| TGCAACAGATTACTAAAGCT+AGG | 0.531119 | 7.3:+50829460 | MS.gene051280:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.532673 | 7.3:+50829461 | MS.gene051280:CDS |
| AGTTGAACATTCGTATGAGT+TGG | 0.539489 | 7.3:+50829523 | MS.gene051280:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.550366 | 7.3:+50829626 | MS.gene051280:CDS |
| CGAAGCCATGACTAGGAAGA+AGG | 0.551367 | 7.3:+50829185 | None:intergenic |
| GAAGAAAAGTATCATCAAGA+AGG | 0.556586 | 7.3:+50829263 | MS.gene051280:CDS |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 7.3:-50829319 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 7.3:+50829241 | MS.gene051280:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 7.3:-50829315 | None:intergenic |
| TTAATTCTAAAACAGAGGTG+TGG | 0.602716 | 7.3:+50829343 | MS.gene051280:CDS |
| TACATTGCGAAGCCATGACT+AGG | 0.618148 | 7.3:+50829178 | None:intergenic |
| AGAGGTGTGGCCAAATCTGG+AGG | 0.647190 | 7.3:+50829356 | MS.gene051280:CDS |
| AAGCAAAACAGATGATTGTG+AGG | 0.652167 | 7.3:+50829379 | MS.gene051280:CDS |
| TTGGTACCTGCAAAACAACA+CGG | 0.687144 | 7.3:+50829542 | MS.gene051280:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTAGAATTAAAAGGAT+CGG | - | chr7.3:50829337-50829356 | None:intergenic | 20.0% |
| !! | GTTAATTGCAAAAAGTATGA+AGG | + | chr7.3:50829608-50829627 | MS.gene051280:CDS | 25.0% |
| !!! | ACCTCTGTTTTAGAATTAAA+AGG | - | chr7.3:50829342-50829361 | None:intergenic | 25.0% |
| !!! | TCCTTTTAATTCTAAAACAG+AGG | + | chr7.3:50829338-50829357 | MS.gene051280:CDS | 25.0% |
| CTTTAGTAATCTGTTGCAAA+AGG | - | chr7.3:50829458-50829477 | None:intergenic | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | - | chr7.3:50829220-50829239 | None:intergenic | 30.0% | |
| ! | TGTTTCTGATTTGAAAGACT+TGG | + | chr7.3:50829581-50829600 | MS.gene051280:CDS | 30.0% |
| !! | GAAGAAAAGTATCATCAAGA+AGG | + | chr7.3:50829263-50829282 | MS.gene051280:CDS | 30.0% |
| !! | TTAATTCTAAAACAGAGGTG+TGG | + | chr7.3:50829343-50829362 | MS.gene051280:CDS | 30.0% |
| AAGCAAAACAGATGATTGTG+AGG | + | chr7.3:50829379-50829398 | MS.gene051280:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | chr7.3:50829288-50829307 | MS.gene051280:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | - | chr7.3:50829229-50829248 | None:intergenic | 35.0% | |
| AGTTGAACATTCGTATGAGT+TGG | + | chr7.3:50829523-50829542 | MS.gene051280:CDS | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | + | chr7.3:50829224-50829243 | MS.gene051280:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | + | chr7.3:50829461-50829480 | MS.gene051280:CDS | 35.0% | |
| TCAGAAACAGTCATATCATC+TGG | - | chr7.3:50829570-50829589 | None:intergenic | 35.0% | |
| TGAGAGAAAAAATGTGAACC+AGG | + | chr7.3:50829425-50829444 | MS.gene051280:CDS | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | + | chr7.3:50829460-50829479 | MS.gene051280:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | - | chr7.3:50829253-50829272 | None:intergenic | 35.0% |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr7.3:50829626-50829645 | MS.gene051280:CDS | 35.0% |
| AGGATCGGAAATTATAGCAC+AGG | - | chr7.3:50829322-50829341 | None:intergenic | 40.0% | |
| CTTGTCTTTGCTTTCTCAGT+TGG | - | chr7.3:50829488-50829507 | None:intergenic | 40.0% | |
| GTTGCAAAAGGAAACTTTCC+TGG | - | chr7.3:50829446-50829465 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+CGG | + | chr7.3:50829542-50829561 | MS.gene051280:CDS | 40.0% | |
| ! | TGTTTTGCTTCCTCCAGATT+TGG | - | chr7.3:50829369-50829388 | None:intergenic | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | + | chr7.3:50829241-50829260 | MS.gene051280:CDS | 45.0% | |
| TCGGAAATTATAGCACAGGC+TGG | - | chr7.3:50829318-50829337 | None:intergenic | 45.0% | |
| ! | AACAGAGGTGTGGCCAAATC+TGG | + | chr7.3:50829353-50829372 | MS.gene051280:CDS | 50.0% |
| ! | AGAGGTGTGGCCAAATCTGG+AGG | + | chr7.3:50829356-50829375 | MS.gene051280:CDS | 55.0% |
| !!! | CTGGCACCGTGTTGTTTTGC+AGG | - | chr7.3:50829551-50829570 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 50829192 | 50829683 | 50829192 | ID=MS.gene051280 |
| chr7.3 | mRNA | 50829192 | 50829683 | 50829192 | ID=MS.gene051280.t1;Parent=MS.gene051280 |
| chr7.3 | exon | 50829192 | 50829683 | 50829192 | ID=MS.gene051280.t1.exon1;Parent=MS.gene051280.t1 |
| chr7.3 | CDS | 50829192 | 50829683 | 50829192 | ID=cds.MS.gene051280.t1;Parent=MS.gene051280.t1 |
| Gene Sequence |
| Protein sequence |
