Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051281.t1 | XP_013468829.1 | 89.9 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 1.70E-70 | 275.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051281.t1 | Q9FJK3 | 46.2 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 1.3e-28 | 127.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051281.t1 | A0A072VMQ1 | 89.9 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 1.2e-70 | 275.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051281.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051281.t1 | MTR_1g077320 | 89.937 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 9.14e-95 | 271 |
| MS.gene051281.t1 | MTR_4g028720 | 85.535 | 159 | 23 | 0 | 1 | 159 | 1 | 159 | 6.88e-93 | 266 |
| MS.gene051281.t1 | MTR_7g011950 | 87.421 | 159 | 20 | 0 | 1 | 159 | 1 | 159 | 7.60e-93 | 266 |
| MS.gene051281.t1 | MTR_1g077390 | 71.338 | 157 | 45 | 0 | 1 | 157 | 1 | 157 | 4.18e-78 | 229 |
| MS.gene051281.t1 | MTR_5g047580 | 67.308 | 156 | 51 | 0 | 1 | 156 | 1 | 156 | 6.71e-73 | 216 |
| MS.gene051281.t1 | MTR_1g077300 | 72.956 | 159 | 41 | 1 | 1 | 159 | 1 | 157 | 9.84e-73 | 215 |
| MS.gene051281.t1 | MTR_1g084950 | 71.795 | 156 | 44 | 0 | 1 | 156 | 1 | 156 | 7.23e-72 | 213 |
| MS.gene051281.t1 | MTR_1g090697 | 57.419 | 155 | 66 | 0 | 1 | 155 | 1 | 155 | 1.42e-58 | 179 |
| MS.gene051281.t1 | MTR_1g090710 | 55.128 | 156 | 69 | 1 | 1 | 155 | 1 | 156 | 5.73e-56 | 173 |
| MS.gene051281.t1 | MTR_1g090783 | 54.902 | 153 | 69 | 0 | 3 | 155 | 2 | 154 | 2.32e-55 | 171 |
| MS.gene051281.t1 | MTR_5g047560 | 56.410 | 156 | 52 | 1 | 1 | 156 | 1 | 140 | 5.55e-55 | 170 |
| MS.gene051281.t1 | MTR_3g065100 | 49.682 | 157 | 78 | 1 | 1 | 156 | 1 | 157 | 2.21e-39 | 133 |
| MS.gene051281.t1 | MTR_4g032620 | 38.710 | 155 | 93 | 2 | 1 | 153 | 1 | 155 | 6.37e-37 | 127 |
| MS.gene051281.t1 | MTR_3g466980 | 43.137 | 153 | 86 | 1 | 1 | 152 | 1 | 153 | 1.22e-36 | 125 |
| MS.gene051281.t1 | MTR_5g075380 | 39.744 | 156 | 93 | 1 | 1 | 155 | 1 | 156 | 1.59e-36 | 124 |
| MS.gene051281.t1 | MTR_4g063790 | 39.623 | 159 | 94 | 2 | 1 | 157 | 1 | 159 | 3.64e-35 | 120 |
| MS.gene051281.t1 | MTR_3g466830 | 42.675 | 157 | 89 | 1 | 3 | 158 | 2 | 158 | 1.08e-33 | 116 |
| MS.gene051281.t1 | MTR_4g031910 | 44.000 | 150 | 82 | 2 | 1 | 148 | 1 | 150 | 1.33e-32 | 115 |
| MS.gene051281.t1 | MTR_3g466890 | 42.208 | 154 | 88 | 1 | 3 | 155 | 2 | 155 | 4.91e-32 | 112 |
| MS.gene051281.t1 | MTR_3g031240 | 36.810 | 163 | 93 | 3 | 1 | 157 | 1 | 159 | 1.21e-31 | 112 |
| MS.gene051281.t1 | MTR_8g036130 | 38.994 | 159 | 93 | 2 | 1 | 155 | 1 | 159 | 7.48e-31 | 112 |
| MS.gene051281.t1 | MTR_4g032260 | 41.875 | 160 | 91 | 2 | 1 | 158 | 1 | 160 | 7.53e-29 | 104 |
| MS.gene051281.t1 | MTR_2g016210 | 38.994 | 159 | 95 | 2 | 1 | 157 | 1 | 159 | 2.32e-28 | 105 |
| MS.gene051281.t1 | MTR_3g467080 | 34.783 | 161 | 103 | 2 | 1 | 159 | 1 | 161 | 1.30e-27 | 101 |
| MS.gene051281.t1 | MTR_4g032290 | 40.994 | 161 | 93 | 2 | 1 | 159 | 1 | 161 | 2.77e-27 | 102 |
| MS.gene051281.t1 | MTR_3g031100 | 35.669 | 157 | 99 | 2 | 1 | 155 | 1 | 157 | 1.54e-26 | 100 |
| MS.gene051281.t1 | MTR_2g035610 | 42.735 | 117 | 66 | 1 | 1 | 116 | 1 | 117 | 1.34e-25 | 94.7 |
| MS.gene051281.t1 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 4.22e-22 | 86.7 |
| MS.gene051281.t1 | MTR_2g035580 | 32.258 | 155 | 104 | 1 | 1 | 154 | 1 | 155 | 1.82e-21 | 85.5 |
| MS.gene051281.t1 | MTR_3g466900 | 39.683 | 126 | 73 | 2 | 32 | 155 | 2 | 126 | 1.15e-19 | 80.1 |
| MS.gene051281.t1 | MTR_3g466930 | 37.600 | 125 | 77 | 1 | 32 | 155 | 2 | 126 | 1.17e-19 | 80.1 |
| MS.gene051281.t1 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 1 | 73 | 1 | 71 | 1.94e-19 | 77.8 |
| MS.gene051281.t1 | MTR_4g019670 | 35.000 | 120 | 74 | 2 | 1 | 116 | 1 | 120 | 2.45e-16 | 75.5 |
| MS.gene051281.t1 | MTR_7g106510 | 34.259 | 108 | 70 | 1 | 3 | 109 | 6 | 113 | 1.00e-15 | 73.6 |
| MS.gene051281.t1 | MTR_7g055800 | 33.333 | 114 | 49 | 2 | 1 | 114 | 1 | 87 | 3.92e-14 | 64.3 |
| MS.gene051281.t1 | MTR_1g033370 | 43.617 | 94 | 52 | 1 | 64 | 156 | 28 | 121 | 4.28e-14 | 65.1 |
| MS.gene051281.t1 | MTR_1g033930 | 40.625 | 96 | 56 | 1 | 64 | 158 | 22 | 117 | 5.06e-14 | 65.1 |
| MS.gene051281.t1 | MTR_1g033790 | 41.667 | 96 | 55 | 1 | 64 | 158 | 22 | 117 | 1.47e-13 | 63.9 |
| MS.gene051281.t1 | MTR_6g005450 | 31.250 | 112 | 71 | 3 | 1 | 106 | 1 | 112 | 2.29e-12 | 63.9 |
| MS.gene051281.t1 | MTR_7g055940 | 34.286 | 105 | 42 | 3 | 1 | 105 | 1 | 78 | 4.73e-12 | 58.5 |
| MS.gene051281.t1 | MTR_3g093900 | 26.974 | 152 | 106 | 2 | 3 | 151 | 6 | 155 | 7.54e-12 | 62.4 |
| MS.gene051281.t1 | MTR_7g055790 | 35.780 | 109 | 53 | 2 | 1 | 109 | 59 | 150 | 1.23e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051281.t1 | AT5G48670 | 46.154 | 156 | 83 | 1 | 1 | 155 | 1 | 156 | 1.58e-34 | 123 |
| MS.gene051281.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 6.14e-31 | 111 |
| MS.gene051281.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 3.26e-30 | 110 |
| MS.gene051281.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 4.76e-30 | 110 |
| MS.gene051281.t1 | AT5G26630 | 38.750 | 160 | 97 | 1 | 1 | 159 | 1 | 160 | 2.27e-29 | 107 |
| MS.gene051281.t1 | AT1G65330 | 36.735 | 147 | 92 | 1 | 3 | 148 | 2 | 148 | 3.91e-27 | 103 |
| MS.gene051281.t1 | AT1G65300 | 36.111 | 144 | 91 | 1 | 3 | 145 | 2 | 145 | 1.28e-26 | 101 |
| MS.gene051281.t1 | AT5G26650 | 35.948 | 153 | 97 | 1 | 4 | 155 | 2 | 154 | 2.64e-25 | 99.8 |
| MS.gene051281.t1 | AT5G27960 | 35.294 | 153 | 98 | 1 | 4 | 155 | 2 | 154 | 5.19e-24 | 95.5 |
| MS.gene051281.t1 | AT2G28700 | 34.899 | 149 | 96 | 1 | 1 | 148 | 1 | 149 | 3.21e-21 | 88.2 |
| MS.gene051281.t1 | AT1G31630 | 29.677 | 155 | 107 | 2 | 3 | 156 | 2 | 155 | 5.00e-21 | 87.8 |
| MS.gene051281.t1 | AT5G27810 | 43.299 | 97 | 54 | 1 | 29 | 124 | 1 | 97 | 1.15e-20 | 82.0 |
| MS.gene051281.t1 | AT1G22590 | 28.395 | 162 | 100 | 3 | 1 | 155 | 1 | 153 | 2.69e-20 | 82.4 |
| MS.gene051281.t1 | AT1G31640 | 33.333 | 150 | 98 | 2 | 3 | 151 | 2 | 150 | 1.06e-19 | 85.1 |
| MS.gene051281.t1 | AT5G26580 | 33.133 | 166 | 88 | 3 | 1 | 156 | 1 | 153 | 2.61e-17 | 77.8 |
| MS.gene051281.t1 | AT5G58890 | 36.036 | 111 | 63 | 3 | 1 | 104 | 1 | 110 | 1.90e-14 | 69.3 |
| MS.gene051281.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.33e-13 | 67.4 |
| MS.gene051281.t1 | AT5G06500 | 32.468 | 154 | 89 | 4 | 1 | 148 | 1 | 145 | 2.30e-12 | 63.2 |
| MS.gene051281.t1 | AT5G55690 | 34.568 | 81 | 46 | 2 | 1 | 74 | 1 | 81 | 8.53e-11 | 58.9 |
| MS.gene051281.t1 | AT5G55690 | 34.568 | 81 | 46 | 2 | 1 | 74 | 1 | 81 | 8.53e-11 | 58.9 |
Find 30 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCAAAAGGAAACTTTCT+TGG | 0.253590 | 7.3:-50818267 | None:intergenic |
| AACAGAGGTGTGGCCAAATA+TGG | 0.297138 | 7.3:+50818177 | MS.gene051281:CDS |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 7.3:-50818041 | None:intergenic |
| CTTGTCTTTGCTTTCTCAGT+TGG | 0.347479 | 7.3:-50818309 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.353862 | 7.3:-50818050 | None:intergenic |
| CTCCAATGATTCAACAAGAA+AGG | 0.407412 | 7.3:+50818048 | MS.gene051281:CDS |
| TCAGAAAGAGTCATATCATT+TGG | 0.410486 | 7.3:-50818391 | None:intergenic |
| AAATATGGAGGAAGCAAAAC+AGG | 0.429725 | 7.3:+50818192 | MS.gene051281:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 7.3:-50818279 | None:intergenic |
| CAGAACTCATATATGAAAGA+TGG | 0.456034 | 7.3:+50818229 | MS.gene051281:CDS |
| TCTTTCTGATTTGAAAGACT+TGG | 0.461416 | 7.3:+50818405 | MS.gene051281:CDS |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7.3:-50818014 | None:intergenic |
| ACCTCTGTTTCAGAATCAAA+AGG | 0.516061 | 7.3:-50818163 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 7.3:+50818112 | MS.gene051281:CDS |
| TGCAACAGATTACTAAAGCT+AGG | 0.531119 | 7.3:+50818284 | MS.gene051281:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.532673 | 7.3:+50818285 | MS.gene051281:CDS |
| AGAACTCATATATGAAAGAT+GGG | 0.538835 | 7.3:+50818230 | MS.gene051281:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.550366 | 7.3:+50818450 | MS.gene051281:CDS |
| CGAAGCCATGACTAGGAAGA+AGG | 0.551367 | 7.3:+50818009 | None:intergenic |
| GAAGAAAAGTATCATCAAGA+AGG | 0.556586 | 7.3:+50818087 | MS.gene051281:CDS |
| AGCTGAACATTTGTATGAGT+TGG | 0.558893 | 7.3:+50818347 | MS.gene051281:CDS |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 7.3:-50818143 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 7.3:+50818065 | MS.gene051281:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 7.3:-50818139 | None:intergenic |
| TGTTTCAGAATCAAAAGGAT+CGG | 0.599035 | 7.3:-50818158 | None:intergenic |
| TACATTGCGAAGCCATGACT+AGG | 0.618148 | 7.3:+50818002 | None:intergenic |
| TTGATTCTGAAACAGAGGTG+TGG | 0.630350 | 7.3:+50818167 | MS.gene051281:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.635256 | 7.3:+50818203 | MS.gene051281:CDS |
| AGAGGTGTGGCCAAATATGG+AGG | 0.650352 | 7.3:+50818180 | MS.gene051281:CDS |
| TTGGTACCTGCAAAACAACA+TGG | 0.699539 | 7.3:+50818366 | MS.gene051281:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGAACTCATATATGAAAGAT+GGG | + | chr7.3:50818230-50818249 | MS.gene051281:CDS | 25.0% |
| !! | GTTAATTGCAAAAAGTATGA+AGG | + | chr7.3:50818432-50818451 | MS.gene051281:CDS | 25.0% |
| CAGAACTCATATATGAAAGA+TGG | + | chr7.3:50818229-50818248 | MS.gene051281:CDS | 30.0% | |
| CTTTAGTAATCTGTTGCAAA+AGG | - | chr7.3:50818282-50818301 | None:intergenic | 30.0% | |
| TCAGAAAGAGTCATATCATT+TGG | - | chr7.3:50818394-50818413 | None:intergenic | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | - | chr7.3:50818044-50818063 | None:intergenic | 30.0% | |
| TGTTTCAGAATCAAAAGGAT+CGG | - | chr7.3:50818161-50818180 | None:intergenic | 30.0% | |
| ! | TCTTTCTGATTTGAAAGACT+TGG | + | chr7.3:50818405-50818424 | MS.gene051281:CDS | 30.0% |
| !! | GAAGAAAAGTATCATCAAGA+AGG | + | chr7.3:50818087-50818106 | MS.gene051281:CDS | 30.0% |
| AAATATGGAGGAAGCAAAAC+AGG | + | chr7.3:50818192-50818211 | MS.gene051281:CDS | 35.0% | |
| ACCTCTGTTTCAGAATCAAA+AGG | - | chr7.3:50818166-50818185 | None:intergenic | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | chr7.3:50818112-50818131 | MS.gene051281:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | - | chr7.3:50818053-50818072 | None:intergenic | 35.0% | |
| AGCTGAACATTTGTATGAGT+TGG | + | chr7.3:50818347-50818366 | MS.gene051281:CDS | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | + | chr7.3:50818048-50818067 | MS.gene051281:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | + | chr7.3:50818285-50818304 | MS.gene051281:CDS | 35.0% | |
| GTTGCAAAAGGAAACTTTCT+TGG | - | chr7.3:50818270-50818289 | None:intergenic | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | + | chr7.3:50818284-50818303 | MS.gene051281:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | - | chr7.3:50818077-50818096 | None:intergenic | 35.0% |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr7.3:50818450-50818469 | MS.gene051281:CDS | 35.0% |
| ! | TGTTTTGCTTCCTCCATATT+TGG | - | chr7.3:50818193-50818212 | None:intergenic | 35.0% |
| !!! | TCCTTTTGATTCTGAAACAG+AGG | + | chr7.3:50818162-50818181 | MS.gene051281:CDS | 35.0% |
| AAGCAAAACAGGTGATTGAG+AGG | + | chr7.3:50818203-50818222 | MS.gene051281:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | - | chr7.3:50818146-50818165 | None:intergenic | 40.0% | |
| CTTGTCTTTGCTTTCTCAGT+TGG | - | chr7.3:50818312-50818331 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+TGG | + | chr7.3:50818366-50818385 | MS.gene051281:CDS | 40.0% | |
| !! | TTGATTCTGAAACAGAGGTG+TGG | + | chr7.3:50818167-50818186 | MS.gene051281:CDS | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | + | chr7.3:50818065-50818084 | MS.gene051281:CDS | 45.0% | |
| TCGGAAATTATAGCACAGGC+TGG | - | chr7.3:50818142-50818161 | None:intergenic | 45.0% | |
| ! | AACAGAGGTGTGGCCAAATA+TGG | + | chr7.3:50818177-50818196 | MS.gene051281:CDS | 45.0% |
| !!! | TTGGCACCATGTTGTTTTGC+AGG | - | chr7.3:50818375-50818394 | None:intergenic | 45.0% |
| ! | AGAGGTGTGGCCAAATATGG+AGG | + | chr7.3:50818180-50818199 | MS.gene051281:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 50818016 | 50818495 | 50818016 | ID=MS.gene051281 |
| chr7.3 | mRNA | 50818016 | 50818495 | 50818016 | ID=MS.gene051281.t1;Parent=MS.gene051281 |
| chr7.3 | exon | 50818016 | 50818495 | 50818016 | ID=MS.gene051281.t1.exon1;Parent=MS.gene051281.t1 |
| chr7.3 | CDS | 50818016 | 50818495 | 50818016 | ID=cds.MS.gene051281.t1;Parent=MS.gene051281.t1 |
| Gene Sequence |
| Protein sequence |
