Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051282.t1 | XP_013468829.1 | 89.9 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 9.70E-71 | 276.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051282.t1 | Q9FJK3 | 45.1 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 5.6e-27 | 122.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051282.t1 | A0A072VMQ1 | 89.9 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 7.0e-71 | 276.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051282.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051282.t1 | MTR_1g077320 | 89.937 | 159 | 16 | 0 | 1 | 159 | 1 | 159 | 8.28e-96 | 274 |
| MS.gene051282.t1 | MTR_7g011950 | 86.164 | 159 | 22 | 0 | 1 | 159 | 1 | 159 | 2.77e-91 | 262 |
| MS.gene051282.t1 | MTR_4g028720 | 84.277 | 159 | 25 | 0 | 1 | 159 | 1 | 159 | 2.83e-91 | 262 |
| MS.gene051282.t1 | MTR_1g077390 | 71.338 | 157 | 45 | 0 | 1 | 157 | 1 | 157 | 8.15e-78 | 228 |
| MS.gene051282.t1 | MTR_5g047580 | 68.421 | 152 | 48 | 0 | 1 | 152 | 1 | 152 | 9.95e-73 | 215 |
| MS.gene051282.t1 | MTR_1g077300 | 72.327 | 159 | 42 | 1 | 1 | 159 | 1 | 157 | 1.04e-72 | 215 |
| MS.gene051282.t1 | MTR_1g084950 | 73.026 | 152 | 41 | 0 | 1 | 152 | 1 | 152 | 1.59e-71 | 212 |
| MS.gene051282.t1 | MTR_1g090697 | 57.895 | 152 | 64 | 0 | 1 | 152 | 1 | 152 | 2.73e-58 | 179 |
| MS.gene051282.t1 | MTR_1g090710 | 55.556 | 153 | 67 | 1 | 1 | 152 | 1 | 153 | 7.12e-56 | 172 |
| MS.gene051282.t1 | MTR_1g090783 | 56.000 | 150 | 66 | 0 | 3 | 152 | 2 | 151 | 3.44e-55 | 171 |
| MS.gene051282.t1 | MTR_5g047560 | 57.237 | 152 | 49 | 1 | 1 | 152 | 1 | 136 | 1.77e-54 | 169 |
| MS.gene051282.t1 | MTR_3g065100 | 50.667 | 150 | 73 | 1 | 1 | 149 | 1 | 150 | 1.70e-38 | 131 |
| MS.gene051282.t1 | MTR_5g075380 | 39.610 | 154 | 92 | 1 | 1 | 153 | 1 | 154 | 5.24e-37 | 125 |
| MS.gene051282.t1 | MTR_4g032620 | 38.710 | 155 | 93 | 2 | 1 | 153 | 1 | 155 | 1.40e-36 | 126 |
| MS.gene051282.t1 | MTR_3g466980 | 43.137 | 153 | 86 | 1 | 1 | 152 | 1 | 153 | 1.42e-36 | 125 |
| MS.gene051282.t1 | MTR_4g063790 | 39.623 | 159 | 94 | 2 | 1 | 157 | 1 | 159 | 2.16e-34 | 119 |
| MS.gene051282.t1 | MTR_3g466830 | 43.333 | 150 | 84 | 1 | 3 | 151 | 2 | 151 | 2.00e-33 | 115 |
| MS.gene051282.t1 | MTR_4g031910 | 44.667 | 150 | 81 | 2 | 1 | 148 | 1 | 150 | 8.95e-33 | 116 |
| MS.gene051282.t1 | MTR_3g466890 | 42.384 | 151 | 86 | 1 | 3 | 152 | 2 | 152 | 2.32e-32 | 113 |
| MS.gene051282.t1 | MTR_3g031240 | 37.179 | 156 | 96 | 2 | 1 | 154 | 1 | 156 | 2.49e-30 | 108 |
| MS.gene051282.t1 | MTR_8g036130 | 38.608 | 158 | 93 | 2 | 1 | 154 | 1 | 158 | 4.06e-30 | 110 |
| MS.gene051282.t1 | MTR_4g032260 | 42.500 | 160 | 90 | 2 | 1 | 158 | 1 | 160 | 2.88e-29 | 105 |
| MS.gene051282.t1 | MTR_2g016210 | 38.365 | 159 | 96 | 2 | 1 | 157 | 1 | 159 | 1.63e-28 | 105 |
| MS.gene051282.t1 | MTR_4g032290 | 44.000 | 150 | 82 | 2 | 1 | 148 | 1 | 150 | 1.30e-27 | 103 |
| MS.gene051282.t1 | MTR_3g467080 | 35.065 | 154 | 98 | 2 | 1 | 152 | 1 | 154 | 9.48e-27 | 99.0 |
| MS.gene051282.t1 | MTR_3g031100 | 36.306 | 157 | 96 | 3 | 1 | 154 | 1 | 156 | 2.66e-26 | 100 |
| MS.gene051282.t1 | MTR_2g035610 | 42.735 | 117 | 66 | 1 | 1 | 116 | 1 | 117 | 2.43e-25 | 94.4 |
| MS.gene051282.t1 | MTR_2g035580 | 32.258 | 155 | 104 | 1 | 1 | 154 | 1 | 155 | 3.35e-21 | 84.7 |
| MS.gene051282.t1 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 4.36e-21 | 84.0 |
| MS.gene051282.t1 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 1 | 73 | 1 | 71 | 1.59e-19 | 77.8 |
| MS.gene051282.t1 | MTR_3g466900 | 38.525 | 122 | 74 | 1 | 32 | 152 | 2 | 123 | 1.67e-19 | 79.3 |
| MS.gene051282.t1 | MTR_3g466930 | 37.705 | 122 | 75 | 1 | 32 | 152 | 2 | 123 | 1.67e-19 | 79.3 |
| MS.gene051282.t1 | MTR_4g019670 | 35.000 | 120 | 74 | 2 | 1 | 116 | 1 | 120 | 3.31e-16 | 75.1 |
| MS.gene051282.t1 | MTR_7g106510 | 30.128 | 156 | 103 | 3 | 3 | 155 | 6 | 158 | 4.77e-16 | 74.7 |
| MS.gene051282.t1 | MTR_7g055800 | 33.333 | 114 | 49 | 2 | 1 | 114 | 1 | 87 | 2.87e-14 | 64.7 |
| MS.gene051282.t1 | MTR_6g005450 | 32.143 | 112 | 70 | 3 | 1 | 106 | 1 | 112 | 6.62e-13 | 65.5 |
| MS.gene051282.t1 | MTR_1g033370 | 42.857 | 91 | 51 | 1 | 64 | 153 | 28 | 118 | 8.37e-13 | 62.0 |
| MS.gene051282.t1 | MTR_1g033930 | 40.000 | 90 | 53 | 1 | 64 | 152 | 22 | 111 | 1.01e-12 | 61.6 |
| MS.gene051282.t1 | MTR_7g055940 | 34.286 | 105 | 42 | 3 | 1 | 105 | 1 | 78 | 3.59e-12 | 58.9 |
| MS.gene051282.t1 | MTR_1g033790 | 40.659 | 91 | 53 | 1 | 64 | 153 | 22 | 112 | 8.77e-12 | 59.3 |
| MS.gene051282.t1 | MTR_7g055790 | 35.780 | 109 | 53 | 2 | 1 | 109 | 59 | 150 | 1.28e-11 | 59.3 |
| MS.gene051282.t1 | MTR_3g093900 | 26.000 | 150 | 110 | 1 | 3 | 151 | 6 | 155 | 2.03e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051282.t1 | AT5G48670 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 1.16e-32 | 118 |
| MS.gene051282.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 4.49e-31 | 111 |
| MS.gene051282.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 1.83e-30 | 110 |
| MS.gene051282.t1 | AT3G05860 | 39.597 | 149 | 89 | 1 | 1 | 148 | 1 | 149 | 2.54e-30 | 110 |
| MS.gene051282.t1 | AT5G26630 | 39.216 | 153 | 92 | 1 | 1 | 152 | 1 | 153 | 2.97e-29 | 107 |
| MS.gene051282.t1 | AT1G65300 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 1.81e-27 | 103 |
| MS.gene051282.t1 | AT1G65330 | 36.735 | 147 | 92 | 1 | 3 | 148 | 2 | 148 | 4.94e-27 | 102 |
| MS.gene051282.t1 | AT5G26650 | 35.526 | 152 | 97 | 1 | 4 | 154 | 2 | 153 | 3.43e-24 | 96.7 |
| MS.gene051282.t1 | AT5G27960 | 43.925 | 107 | 59 | 1 | 4 | 109 | 2 | 108 | 1.54e-23 | 94.4 |
| MS.gene051282.t1 | AT2G28700 | 34.228 | 149 | 97 | 1 | 1 | 148 | 1 | 149 | 2.48e-20 | 85.9 |
| MS.gene051282.t1 | AT1G22590 | 33.043 | 115 | 76 | 1 | 1 | 114 | 1 | 115 | 3.59e-20 | 82.0 |
| MS.gene051282.t1 | AT1G31630 | 29.139 | 151 | 105 | 2 | 3 | 152 | 2 | 151 | 7.36e-20 | 84.7 |
| MS.gene051282.t1 | AT5G27810 | 41.667 | 96 | 55 | 1 | 29 | 123 | 1 | 96 | 4.92e-19 | 78.2 |
| MS.gene051282.t1 | AT1G31640 | 39.640 | 111 | 65 | 2 | 3 | 112 | 2 | 111 | 1.18e-18 | 82.0 |
| MS.gene051282.t1 | AT5G26580 | 38.532 | 109 | 54 | 1 | 1 | 109 | 1 | 96 | 2.59e-17 | 77.8 |
| MS.gene051282.t1 | AT5G58890 | 36.036 | 111 | 63 | 3 | 1 | 104 | 1 | 110 | 2.30e-14 | 68.9 |
| MS.gene051282.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 2.64e-13 | 66.6 |
| MS.gene051282.t1 | AT5G06500 | 32.468 | 154 | 89 | 4 | 1 | 148 | 1 | 145 | 2.62e-12 | 62.8 |
| MS.gene051282.t1 | AT5G55690 | 34.568 | 81 | 46 | 2 | 1 | 74 | 1 | 81 | 9.05e-11 | 58.9 |
| MS.gene051282.t1 | AT5G55690 | 34.568 | 81 | 46 | 2 | 1 | 74 | 1 | 81 | 9.05e-11 | 58.9 |
Find 30 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTGCAAAAGGAAACTTTCT+TGG | 0.253590 | 7.3:-50802659 | None:intergenic |
| AACAGAGGTGTGGCCAAATA+TGG | 0.297138 | 7.3:+50802569 | MS.gene051282:CDS |
| TGTTGAATCATTGGAGATAA+AGG | 0.341938 | 7.3:-50802433 | None:intergenic |
| CTTGTCTTTGCTTTCTCAGT+TGG | 0.347479 | 7.3:-50802701 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.353862 | 7.3:-50802442 | None:intergenic |
| CTCCAATGATTCAACAAGAA+AGG | 0.407412 | 7.3:+50802440 | MS.gene051282:CDS |
| TCAGATAGAGTCATATCATT+TGG | 0.419545 | 7.3:-50802783 | None:intergenic |
| AAATATGGAGGAAGCAAAAC+AGG | 0.429725 | 7.3:+50802584 | MS.gene051282:CDS |
| CAGAACTCATATATTAAAGA+TGG | 0.434080 | 7.3:+50802621 | MS.gene051282:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 7.3:-50802671 | None:intergenic |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7.3:-50802406 | None:intergenic |
| TCTATCTGATTTGAAAGACT+TGG | 0.515931 | 7.3:+50802797 | MS.gene051282:CDS |
| ACCTCTGTTTCAGAATCAAA+AGG | 0.516061 | 7.3:-50802555 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 7.3:+50802504 | MS.gene051282:CDS |
| TGCAACAGATTACTAAAGCT+AGG | 0.531119 | 7.3:+50802676 | MS.gene051282:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.532673 | 7.3:+50802677 | MS.gene051282:CDS |
| AGCTGAACATTCGTATGAGT+TGG | 0.547236 | 7.3:+50802739 | MS.gene051282:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.550366 | 7.3:+50802842 | MS.gene051282:CDS |
| CGAAGCCATGACTAGGAAGA+AGG | 0.551367 | 7.3:+50802401 | None:intergenic |
| GAAGAAAAGTATCATCAAGA+AGG | 0.556586 | 7.3:+50802479 | MS.gene051282:CDS |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 7.3:-50802535 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 7.3:+50802457 | MS.gene051282:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 7.3:-50802531 | None:intergenic |
| AGAACTCATATATTAAAGAT+GGG | 0.572258 | 7.3:+50802622 | MS.gene051282:CDS |
| TGTTTCAGAATCAAAAGGAT+CGG | 0.599035 | 7.3:-50802550 | None:intergenic |
| TACATTGCGAAGCCATGACT+AGG | 0.618148 | 7.3:+50802394 | None:intergenic |
| TTGATTCTGAAACAGAGGTG+TGG | 0.630350 | 7.3:+50802559 | MS.gene051282:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.635256 | 7.3:+50802595 | MS.gene051282:CDS |
| AGAGGTGTGGCCAAATATGG+AGG | 0.650352 | 7.3:+50802572 | MS.gene051282:CDS |
| TTGGTACCTGCAAAACAACA+CGG | 0.687144 | 7.3:+50802758 | MS.gene051282:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAACTCATATATTAAAGAT+GGG | + | chr7.3:50802622-50802641 | MS.gene051282:CDS | 20.0% |
| ! | CAGAACTCATATATTAAAGA+TGG | + | chr7.3:50802621-50802640 | MS.gene051282:CDS | 25.0% |
| !! | GTTAATTGCAAAAAGTATGA+AGG | + | chr7.3:50802824-50802843 | MS.gene051282:CDS | 25.0% |
| CTTTAGTAATCTGTTGCAAA+AGG | - | chr7.3:50802674-50802693 | None:intergenic | 30.0% | |
| TCAGATAGAGTCATATCATT+TGG | - | chr7.3:50802786-50802805 | None:intergenic | 30.0% | |
| TGTTGAATCATTGGAGATAA+AGG | - | chr7.3:50802436-50802455 | None:intergenic | 30.0% | |
| TGTTTCAGAATCAAAAGGAT+CGG | - | chr7.3:50802553-50802572 | None:intergenic | 30.0% | |
| ! | TCTATCTGATTTGAAAGACT+TGG | + | chr7.3:50802797-50802816 | MS.gene051282:CDS | 30.0% |
| !! | GAAGAAAAGTATCATCAAGA+AGG | + | chr7.3:50802479-50802498 | MS.gene051282:CDS | 30.0% |
| AAATATGGAGGAAGCAAAAC+AGG | + | chr7.3:50802584-50802603 | MS.gene051282:CDS | 35.0% | |
| ACCTCTGTTTCAGAATCAAA+AGG | - | chr7.3:50802558-50802577 | None:intergenic | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | chr7.3:50802504-50802523 | MS.gene051282:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | - | chr7.3:50802445-50802464 | None:intergenic | 35.0% | |
| CTCCAATGATTCAACAAGAA+AGG | + | chr7.3:50802440-50802459 | MS.gene051282:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | + | chr7.3:50802677-50802696 | MS.gene051282:CDS | 35.0% | |
| GTTGCAAAAGGAAACTTTCT+TGG | - | chr7.3:50802662-50802681 | None:intergenic | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | + | chr7.3:50802676-50802695 | MS.gene051282:CDS | 35.0% | |
| ! | ACTTTTCTTCCTCTTCTTGT+AGG | - | chr7.3:50802469-50802488 | None:intergenic | 35.0% |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr7.3:50802842-50802861 | MS.gene051282:CDS | 35.0% |
| ! | TGTTTTGCTTCCTCCATATT+TGG | - | chr7.3:50802585-50802604 | None:intergenic | 35.0% |
| !!! | TCCTTTTGATTCTGAAACAG+AGG | + | chr7.3:50802554-50802573 | MS.gene051282:CDS | 35.0% |
| AAGCAAAACAGGTGATTGAG+AGG | + | chr7.3:50802595-50802614 | MS.gene051282:CDS | 40.0% | |
| AGCTGAACATTCGTATGAGT+TGG | + | chr7.3:50802739-50802758 | MS.gene051282:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | - | chr7.3:50802538-50802557 | None:intergenic | 40.0% | |
| CTTGTCTTTGCTTTCTCAGT+TGG | - | chr7.3:50802704-50802723 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+CGG | + | chr7.3:50802758-50802777 | MS.gene051282:CDS | 40.0% | |
| !! | TTGATTCTGAAACAGAGGTG+TGG | + | chr7.3:50802559-50802578 | MS.gene051282:CDS | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | + | chr7.3:50802457-50802476 | MS.gene051282:CDS | 45.0% | |
| TCGGAAATTATAGCACAGGC+TGG | - | chr7.3:50802534-50802553 | None:intergenic | 45.0% | |
| ! | AACAGAGGTGTGGCCAAATA+TGG | + | chr7.3:50802569-50802588 | MS.gene051282:CDS | 45.0% |
| ! | AGAGGTGTGGCCAAATATGG+AGG | + | chr7.3:50802572-50802591 | MS.gene051282:CDS | 50.0% |
| !!! | TTGGCACCGTGTTGTTTTGC+AGG | - | chr7.3:50802767-50802786 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 50802408 | 50802887 | 50802408 | ID=MS.gene051282 |
| chr7.3 | mRNA | 50802408 | 50802887 | 50802408 | ID=MS.gene051282.t1;Parent=MS.gene051282 |
| chr7.3 | exon | 50802408 | 50802887 | 50802408 | ID=MS.gene051282.t1.exon1;Parent=MS.gene051282.t1 |
| chr7.3 | CDS | 50802408 | 50802887 | 50802408 | ID=cds.MS.gene051282.t1;Parent=MS.gene051282.t1 |
| Gene Sequence |
| Protein sequence |
