Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051443.t1 | RHN79737.1 | 61.7 | 243 | 34 | 3 | 5 | 245 | 9 | 194 | 1.10E-53 | 220.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051443.t1 | A0A396JYN9 | 61.7 | 243 | 34 | 3 | 5 | 245 | 9 | 194 | 7.8e-54 | 220.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051443.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051443.t1 | MTR_1g063150 | 62.712 | 236 | 29 | 4 | 38 | 271 | 21 | 199 | 4.10e-89 | 263 |
| MS.gene051443.t1 | MTR_1g085590 | 52.448 | 286 | 83 | 12 | 6 | 269 | 15 | 269 | 5.80e-68 | 213 |
| MS.gene051443.t1 | MTR_1g063140 | 59.777 | 179 | 27 | 5 | 91 | 265 | 1 | 138 | 1.35e-54 | 174 |
| MS.gene051443.t1 | MTR_1g046810 | 44.255 | 235 | 68 | 10 | 53 | 242 | 94 | 310 | 7.28e-41 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAGAGAAAGAAGGGTATT+TGG | 0.170547 | 1.1:+47770414 | None:intergenic |
| AATGAAGGTGAAACAGAATT+AGG | 0.205798 | 1.1:-47769821 | MS.gene051443:CDS |
| CTTAGCACTTGCCGTTTCTT+AGG | 0.240976 | 1.1:+47770139 | None:intergenic |
| CTCTTCAAAACTTTGATTAG+AGG | 0.289539 | 1.1:+47769853 | None:intergenic |
| TGAATCACCGTGGTGCTAAA+AGG | 0.300323 | 1.1:-47770018 | MS.gene051443:CDS |
| AAAGAGAAAGAAGGGTATTT+GGG | 0.359789 | 1.1:+47770415 | None:intergenic |
| ACTTCTAACAAAGCTTTGAA+TGG | 0.400226 | 1.1:-47770232 | MS.gene051443:CDS |
| ACGGTGATTCAAATTGATAT+TGG | 0.408428 | 1.1:+47770030 | None:intergenic |
| TATATCTCGTGGCGGTTTCG+AGG | 0.421737 | 1.1:-47770062 | MS.gene051443:CDS |
| ATGAAGGTGAAACAGAATTA+GGG | 0.423059 | 1.1:-47769820 | MS.gene051443:CDS |
| CTGGAAAACATAAGATCATA+TGG | 0.423840 | 1.1:-47770330 | MS.gene051443:CDS |
| TATTCAGTTCCTAATGATGC+TGG | 0.435072 | 1.1:-47770298 | MS.gene051443:CDS |
| GAAGAGAAGAAGATTAATGA+AGG | 0.440260 | 1.1:-47769836 | MS.gene051443:CDS |
| TCTTCAACAACTAATGATGC+TGG | 0.461941 | 1.1:-47770349 | MS.gene051443:CDS |
| AAGGGTATTTGGGTTGTTGA+AGG | 0.465907 | 1.1:+47770425 | None:intergenic |
| AAACTTTGATTAGAGGCAGC+AGG | 0.484548 | 1.1:+47769860 | None:intergenic |
| AAATTCATCACTTCATCACT+TGG | 0.491144 | 1.1:+47769761 | None:intergenic |
| AGGGTATTTGGGTTGTTGAA+GGG | 0.505337 | 1.1:+47770426 | None:intergenic |
| TGGGTTGTTGAAGGGAGCTA+TGG | 0.513930 | 1.1:+47770434 | None:intergenic |
| AATCAATGAAAGAGAAAGAA+GGG | 0.516168 | 1.1:+47770407 | None:intergenic |
| ATATCTCGTGGCGGTTTCGA+GGG | 0.521594 | 1.1:-47770061 | MS.gene051443:CDS |
| ATCTTCCTCTGCAAGCTATG+AGG | 0.522500 | 1.1:-47770176 | MS.gene051443:CDS |
| TATGATGGATGCTAATATGA+TGG | 0.534594 | 1.1:-47769978 | MS.gene051443:CDS |
| TGATATTGAGAAGACGAAGA+AGG | 0.543732 | 1.1:-47769954 | MS.gene051443:CDS |
| TAATCAATGAAAGAGAAAGA+AGG | 0.544898 | 1.1:+47770406 | None:intergenic |
| TCATAGCTTGCAGAGGAAGA+TGG | 0.548781 | 1.1:+47770178 | None:intergenic |
| ACTTTCTACCACCTAAGAAA+CGG | 0.555851 | 1.1:-47770150 | MS.gene051443:CDS |
| TCATCTTCAAAATCATCACT+TGG | 0.561254 | 1.1:+47769692 | None:intergenic |
| AGCACTTGCCGTTTCTTAGG+TGG | 0.568114 | 1.1:+47770142 | None:intergenic |
| TTCATGTGATGTTTGCAACA+AGG | 0.571040 | 1.1:-47770260 | MS.gene051443:CDS |
| AAATTCTTCACATCACCACT+TGG | 0.573268 | 1.1:+47769731 | None:intergenic |
| TATTGCAGCTGAAACTCTGT+TGG | 0.580874 | 1.1:-47770086 | MS.gene051443:CDS |
| GAGGAAGATGGTGCAACAGT+TGG | 0.627306 | 1.1:+47770190 | None:intergenic |
| ACGAAGAAGGAGAAAGAACA+AGG | 0.647791 | 1.1:-47769941 | MS.gene051443:CDS |
| CTGTTGGATATATCTCGTGG+CGG | 0.650335 | 1.1:-47770070 | MS.gene051443:CDS |
| GTGATCCTCATAGCTTGCAG+AGG | 0.672732 | 1.1:+47770171 | None:intergenic |
| ACTCTGTTGGATATATCTCG+TGG | 0.678764 | 1.1:-47770073 | MS.gene051443:CDS |
| AATATCAATTTGAATCACCG+TGG | 0.727003 | 1.1:-47770028 | MS.gene051443:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTGGTTTTGATATGATTA+GGG | + | chr1.1:47769783-47769802 | None:intergenic | 20.0% |
| !!! | TTTTTGGTTTTGATATGATT+AGG | + | chr1.1:47769784-47769803 | None:intergenic | 20.0% |
| ! | AATCAATGAAAGAGAAAGAA+GGG | + | chr1.1:47769753-47769772 | None:intergenic | 25.0% |
| ! | TAATCAATGAAAGAGAAAGA+AGG | + | chr1.1:47769754-47769773 | None:intergenic | 25.0% |
| !! | AAAACTCTTTTGCAATATGA+TGG | - | chr1.1:47770164-47770183 | MS.gene051443:CDS | 25.0% |
| !! | AGTTGTTGAAGAAAAGTTTT+TGG | + | chr1.1:47769800-47769819 | None:intergenic | 25.0% |
| AAATTCATCACTTCATCACT+TGG | + | chr1.1:47770399-47770418 | None:intergenic | 30.0% | |
| AATATCAATTTGAATCACCG+TGG | - | chr1.1:47770129-47770148 | MS.gene051443:CDS | 30.0% | |
| AATGAAGGTGAAACAGAATT+AGG | - | chr1.1:47770336-47770355 | MS.gene051443:CDS | 30.0% | |
| ACGGTGATTCAAATTGATAT+TGG | + | chr1.1:47770130-47770149 | None:intergenic | 30.0% | |
| ATGAAGGTGAAACAGAATTA+GGG | - | chr1.1:47770337-47770356 | MS.gene051443:CDS | 30.0% | |
| CTCTTCAAAACTTTGATTAG+AGG | + | chr1.1:47770307-47770326 | None:intergenic | 30.0% | |
| CTGGAAAACATAAGATCATA+TGG | - | chr1.1:47769827-47769846 | MS.gene051443:CDS | 30.0% | |
| GAAGAGAAGAAGATTAATGA+AGG | - | chr1.1:47770321-47770340 | MS.gene051443:CDS | 30.0% | |
| TCATCTTCAAAATCATCACT+TGG | + | chr1.1:47770468-47770487 | None:intergenic | 30.0% | |
| !! | AAAGAGAAAGAAGGGTATTT+GGG | + | chr1.1:47769745-47769764 | None:intergenic | 30.0% |
| !! | ACTTCTAACAAAGCTTTGAA+TGG | - | chr1.1:47769925-47769944 | MS.gene051443:CDS | 30.0% |
| !! | TATGATGGATGCTAATATGA+TGG | - | chr1.1:47770179-47770198 | MS.gene051443:CDS | 30.0% |
| !!! | TGGTCAACATGATTAGTTTT+TGG | + | chr1.1:47769706-47769725 | None:intergenic | 30.0% |
| !!! | TTTTGTTTTCCAGCATCATT+AGG | + | chr1.1:47769871-47769890 | None:intergenic | 30.0% |
| AAATTCTTCACATCACCACT+TGG | + | chr1.1:47770429-47770448 | None:intergenic | 35.0% | |
| ACTTTCTACCACCTAAGAAA+CGG | - | chr1.1:47770007-47770026 | MS.gene051443:CDS | 35.0% | |
| TATTCAGTTCCTAATGATGC+TGG | - | chr1.1:47769859-47769878 | MS.gene051443:CDS | 35.0% | |
| TCTTCAACAACTAATGATGC+TGG | - | chr1.1:47769808-47769827 | MS.gene051443:CDS | 35.0% | |
| TGATATTGAGAAGACGAAGA+AGG | - | chr1.1:47770203-47770222 | MS.gene051443:CDS | 35.0% | |
| TTCATGTGATGTTTGCAACA+AGG | - | chr1.1:47769897-47769916 | MS.gene051443:CDS | 35.0% | |
| ! | ATGAATTTTGAGCTTCCAAG+TGG | - | chr1.1:47770411-47770430 | MS.gene051443:CDS | 35.0% |
| !! | GAAAGAGAAAGAAGGGTATT+TGG | + | chr1.1:47769746-47769765 | None:intergenic | 35.0% |
| AAACTTTGATTAGAGGCAGC+AGG | + | chr1.1:47770300-47770319 | None:intergenic | 40.0% | |
| ACGAAGAAGGAGAAAGAACA+AGG | - | chr1.1:47770216-47770235 | MS.gene051443:CDS | 40.0% | |
| ACTCTGTTGGATATATCTCG+TGG | - | chr1.1:47770084-47770103 | MS.gene051443:CDS | 40.0% | |
| TATTGCAGCTGAAACTCTGT+TGG | - | chr1.1:47770071-47770090 | MS.gene051443:CDS | 40.0% | |
| ! | GTTTTTGCCTTTTAGCACCA+CGG | + | chr1.1:47770149-47770168 | None:intergenic | 40.0% |
| !! | AAGGGTATTTGGGTTGTTGA+AGG | + | chr1.1:47769735-47769754 | None:intergenic | 40.0% |
| !! | AGGGTATTTGGGTTGTTGAA+GGG | + | chr1.1:47769734-47769753 | None:intergenic | 40.0% |
| ATCTTCCTCTGCAAGCTATG+AGG | - | chr1.1:47769981-47770000 | MS.gene051443:CDS | 45.0% | |
| CTGTTGGATATATCTCGTGG+CGG | - | chr1.1:47770087-47770106 | MS.gene051443:CDS | 45.0% | |
| ! | CTTAGCACTTGCCGTTTCTT+AGG | + | chr1.1:47770021-47770040 | None:intergenic | 45.0% |
| ! | TCATAGCTTGCAGAGGAAGA+TGG | + | chr1.1:47769982-47770001 | None:intergenic | 45.0% |
| ! | TGAATCACCGTGGTGCTAAA+AGG | - | chr1.1:47770139-47770158 | MS.gene051443:CDS | 45.0% |
| ATATCTCGTGGCGGTTTCGA+GGG | - | chr1.1:47770096-47770115 | MS.gene051443:CDS | 50.0% | |
| GAGGAAGATGGTGCAACAGT+TGG | + | chr1.1:47769970-47769989 | None:intergenic | 50.0% | |
| TATATCTCGTGGCGGTTTCG+AGG | - | chr1.1:47770095-47770114 | MS.gene051443:CDS | 50.0% | |
| TGGGTTGTTGAAGGGAGCTA+TGG | + | chr1.1:47769726-47769745 | None:intergenic | 50.0% | |
| ! | AGCACTTGCCGTTTCTTAGG+TGG | + | chr1.1:47770018-47770037 | None:intergenic | 50.0% |
| ! | GTGATCCTCATAGCTTGCAG+AGG | + | chr1.1:47769989-47770008 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 47769682 | 47770497 | 47769682 | ID=MS.gene051443 |
| chr1.1 | mRNA | 47769682 | 47770497 | 47769682 | ID=MS.gene051443.t1;Parent=MS.gene051443 |
| chr1.1 | exon | 47769682 | 47770497 | 47769682 | ID=MS.gene051443.t1.exon1;Parent=MS.gene051443.t1 |
| chr1.1 | CDS | 47769682 | 47770497 | 47769682 | ID=cds.MS.gene051443.t1;Parent=MS.gene051443.t1 |
| Gene Sequence |
| Protein sequence |
