Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051548.t1 | XP_027333625.1 | 81.1 | 74 | 12 | 2 | 8 | 80 | 171 | 243 | 9.60E-27 | 129 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051548.t1 | Q5XEM9 | 82.8 | 58 | 9 | 1 | 24 | 80 | 203 | 260 | 1.1e-23 | 110.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051548.t1 | A0A068UYW4 | 78.7 | 75 | 14 | 2 | 7 | 80 | 178 | 251 | 1.5e-26 | 127.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene051548.t1 | TF | Alfin-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051548.t1 | MTR_1g015185 | 96.154 | 52 | 2 | 0 | 29 | 80 | 212 | 263 | 1.24e-31 | 110 |
| MS.gene051548.t1 | MTR_8g019520 | 83.333 | 60 | 8 | 2 | 23 | 80 | 197 | 256 | 6.64e-29 | 103 |
| MS.gene051548.t1 | MTR_8g019520 | 83.333 | 60 | 8 | 2 | 23 | 80 | 198 | 257 | 6.71e-29 | 103 |
| MS.gene051548.t1 | MTR_2g040990 | 83.333 | 54 | 7 | 1 | 29 | 80 | 200 | 253 | 2.40e-27 | 99.8 |
| MS.gene051548.t1 | MTR_2g099500 | 88.462 | 52 | 5 | 1 | 29 | 79 | 199 | 250 | 1.12e-26 | 97.8 |
| MS.gene051548.t1 | MTR_4g134510 | 73.684 | 57 | 15 | 0 | 23 | 79 | 198 | 254 | 5.45e-26 | 96.3 |
| MS.gene051548.t1 | MTR_4g015830 | 73.077 | 52 | 14 | 0 | 29 | 80 | 189 | 240 | 3.44e-23 | 88.6 |
| MS.gene051548.t1 | MTR_7g068590 | 71.154 | 52 | 15 | 0 | 29 | 80 | 191 | 242 | 5.88e-23 | 88.2 |
| MS.gene051548.t1 | MTR_8g066480 | 52.830 | 53 | 12 | 2 | 29 | 80 | 106 | 146 | 3.17e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051548.t1 | AT5G20510 | 83.051 | 59 | 9 | 1 | 23 | 80 | 161 | 219 | 9.09e-31 | 107 |
| MS.gene051548.t1 | AT5G20510 | 83.051 | 59 | 9 | 1 | 23 | 80 | 202 | 260 | 1.47e-30 | 108 |
| MS.gene051548.t1 | AT3G42790 | 86.207 | 58 | 7 | 1 | 23 | 79 | 192 | 249 | 5.38e-30 | 106 |
| MS.gene051548.t1 | AT5G26210 | 88.235 | 51 | 6 | 0 | 29 | 79 | 204 | 254 | 4.63e-28 | 101 |
| MS.gene051548.t1 | AT2G02470 | 75.862 | 58 | 14 | 0 | 23 | 80 | 199 | 256 | 1.29e-27 | 100 |
| MS.gene051548.t1 | AT2G02470 | 75.862 | 58 | 14 | 0 | 23 | 80 | 190 | 247 | 1.39e-27 | 100 |
| MS.gene051548.t1 | AT5G05610 | 65.517 | 58 | 19 | 1 | 23 | 79 | 183 | 240 | 8.89e-24 | 90.1 |
| MS.gene051548.t1 | AT5G05610 | 65.517 | 58 | 19 | 1 | 23 | 79 | 183 | 240 | 8.89e-24 | 90.1 |
| MS.gene051548.t1 | AT1G14510 | 68.519 | 54 | 17 | 0 | 24 | 77 | 195 | 248 | 1.29e-22 | 87.4 |
| MS.gene051548.t1 | AT3G11200 | 63.793 | 58 | 20 | 1 | 23 | 79 | 188 | 245 | 2.52e-21 | 84.0 |
| MS.gene051548.t1 | AT3G11200 | 63.793 | 58 | 20 | 1 | 23 | 79 | 175 | 232 | 3.28e-21 | 83.2 |
Find 21 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCAATGCAAATGAAGTTAA+AGG | 0.322830 | 1.3:+6999995 | MS.gene051548:CDS |
| GCTATGCAGCTGATGAGTTC+TGG | 0.348702 | 1.3:+7000090 | MS.gene051548:CDS |
| TTGATATGCTCAGCCCTTGC+CGG | 0.375496 | 1.3:-7000167 | None:intergenic |
| GCAGTGACATTTGCGAGAAA+TGG | 0.391588 | 1.3:+7000117 | MS.gene051548:CDS |
| ATTTGCGAGAAATGGTTTCA+TGG | 0.414893 | 1.3:+7000125 | MS.gene051548:CDS |
| GTTAAAGGACGAGGAGGTTG+AGG | 0.415516 | 1.3:+7000010 | MS.gene051548:CDS |
| GCTCTCTTGTTGCTGCAAGC+TGG | 0.477603 | 1.3:-7000203 | None:intergenic |
| AAGAACACGGAGAGACTTTG+TGG | 0.480234 | 1.3:+7000054 | MS.gene051548:CDS |
| CGTGAAGATTACACCGGCAA+GGG | 0.539345 | 1.3:+7000154 | MS.gene051548:CDS |
| CTTGCAGCAACAAGAGAGCT+CGG | 0.543274 | 1.3:+7000207 | MS.gene051548:CDS |
| AAGGACGAGGAGGTTGAGGA+TGG | 0.556879 | 1.3:+7000014 | MS.gene051548:CDS |
| GGTTGAGGATGGTGTAGGTG+TGG | 0.578977 | 1.3:+7000025 | MS.gene051548:CDS |
| GAGGAGGTTGAGGATGGTGT+AGG | 0.579309 | 1.3:+7000020 | MS.gene051548:CDS |
| GCGTGAAGATTACACCGGCA+AGG | 0.599177 | 1.3:+7000153 | MS.gene051548:CDS |
| GGAGAGACTTTGTGGGCATG+TGG | 0.602496 | 1.3:+7000062 | MS.gene051548:CDS |
| GCAAATGAAGTTAAAGGACG+AGG | 0.616669 | 1.3:+7000001 | MS.gene051548:CDS |
| AGAACACGGAGAGACTTTGT+GGG | 0.627223 | 1.3:+7000055 | MS.gene051548:CDS |
| GGATGGTGTAGGTGTGGACG+AGG | 0.628142 | 1.3:+7000031 | MS.gene051548:CDS |
| AATGAAGTTAAAGGACGAGG+AGG | 0.713570 | 1.3:+7000004 | MS.gene051548:CDS |
| GGTGTGGACGAGGAAGAACA+CGG | 0.729144 | 1.3:+7000041 | MS.gene051548:CDS |
| AAAATGCGTGAAGATTACAC+CGG | 0.768073 | 1.3:+7000148 | MS.gene051548:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGCGTGAAGATTACAC+CGG | + | chr1.3:7000148-7000167 | MS.gene051548:CDS | 35.0% | |
| ATTTGCGAGAAATGGTTTCA+TGG | + | chr1.3:7000125-7000144 | MS.gene051548:CDS | 35.0% | |
| GGCAATGCAAATGAAGTTAA+AGG | + | chr1.3:6999995-7000014 | MS.gene051548:CDS | 35.0% | |
| AATGAAGTTAAAGGACGAGG+AGG | + | chr1.3:7000004-7000023 | MS.gene051548:CDS | 40.0% | |
| GCAAATGAAGTTAAAGGACG+AGG | + | chr1.3:7000001-7000020 | MS.gene051548:CDS | 40.0% | |
| AAGAACACGGAGAGACTTTG+TGG | + | chr1.3:7000054-7000073 | MS.gene051548:CDS | 45.0% | |
| AGAACACGGAGAGACTTTGT+GGG | + | chr1.3:7000055-7000074 | MS.gene051548:CDS | 45.0% | |
| GCAGTGACATTTGCGAGAAA+TGG | + | chr1.3:7000117-7000136 | MS.gene051548:CDS | 45.0% | |
| CGTGAAGATTACACCGGCAA+GGG | + | chr1.3:7000154-7000173 | MS.gene051548:CDS | 50.0% | |
| CTTGCAGCAACAAGAGAGCT+CGG | + | chr1.3:7000207-7000226 | MS.gene051548:CDS | 50.0% | |
| GCTATGCAGCTGATGAGTTC+TGG | + | chr1.3:7000090-7000109 | MS.gene051548:CDS | 50.0% | |
| GTTAAAGGACGAGGAGGTTG+AGG | + | chr1.3:7000010-7000029 | MS.gene051548:CDS | 50.0% | |
| TTGATATGCTCAGCCCTTGC+CGG | - | chr1.3:7000170-7000189 | None:intergenic | 50.0% | |
| AAGGACGAGGAGGTTGAGGA+TGG | + | chr1.3:7000014-7000033 | MS.gene051548:CDS | 55.0% | |
| GAGGAGGTTGAGGATGGTGT+AGG | + | chr1.3:7000020-7000039 | MS.gene051548:CDS | 55.0% | |
| GCGTGAAGATTACACCGGCA+AGG | + | chr1.3:7000153-7000172 | MS.gene051548:CDS | 55.0% | |
| GCTCTCTTGTTGCTGCAAGC+TGG | - | chr1.3:7000206-7000225 | None:intergenic | 55.0% | |
| GGAGAGACTTTGTGGGCATG+TGG | + | chr1.3:7000062-7000081 | MS.gene051548:CDS | 55.0% | |
| GGTGTGGACGAGGAAGAACA+CGG | + | chr1.3:7000041-7000060 | MS.gene051548:CDS | 55.0% | |
| GGTTGAGGATGGTGTAGGTG+TGG | + | chr1.3:7000025-7000044 | MS.gene051548:CDS | 55.0% | |
| GGATGGTGTAGGTGTGGACG+AGG | + | chr1.3:7000031-7000050 | MS.gene051548:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 6999993 | 7000235 | 6999993 | ID=MS.gene051548 |
| chr1.3 | mRNA | 6999993 | 7000235 | 6999993 | ID=MS.gene051548.t1;Parent=MS.gene051548 |
| chr1.3 | exon | 6999993 | 7000235 | 6999993 | ID=MS.gene051548.t1.exon1;Parent=MS.gene051548.t1 |
| chr1.3 | CDS | 6999993 | 7000235 | 6999993 | ID=cds.MS.gene051548.t1;Parent=MS.gene051548.t1 |
| Gene Sequence |
| Protein sequence |
