AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052017

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052017.t1 MTR_2g098880 96.032 252 10 0 1 252 1 252 0.0 503
MS.gene052017.t1 MTR_2g098880 96.032 252 10 0 1 252 13 264 0.0 502
MS.gene052017.t1 MTR_3g006370 36.269 193 103 5 55 243 44 220 3.21e-35 125
MS.gene052017.t1 MTR_2g098920 37.634 186 101 5 60 243 79 251 5.85e-34 123
MS.gene052017.t1 MTR_4g067290 37.222 180 103 4 60 239 75 244 1.28e-31 117
MS.gene052017.t1 MTR_4g067290 37.222 180 103 4 60 239 55 224 1.30e-31 116
MS.gene052017.t1 MTR_4g067290 37.222 180 103 4 60 239 115 284 4.72e-31 116
MS.gene052017.t1 MTR_4g067290 37.158 183 97 6 60 239 55 222 9.62e-31 114
MS.gene052017.t1 MTR_4g067290 37.158 183 97 6 60 239 73 240 1.22e-30 114
MS.gene052017.t1 MTR_4g067290 37.158 183 97 6 60 239 75 242 1.25e-30 114
MS.gene052017.t1 MTR_7g028855 33.775 151 90 5 24 170 84 228 1.22e-11 64.3
MS.gene052017.t1 MTR_1g057920 33.884 121 71 4 32 151 11 123 1.74e-11 60.5
MS.gene052017.t1 MTR_1g070355 33.333 102 66 2 65 165 78 178 2.40e-11 63.5
MS.gene052017.t1 MTR_2g070590 34.286 105 66 2 61 164 17 119 6.81e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052017.t1 AT2G43280 43.077 195 89 6 54 243 15 192 5.24e-42 142
MS.gene052017.t1 AT3G07500 39.894 188 106 3 56 243 28 208 1.56e-39 136
MS.gene052017.t1 AT2G43280 44.509 173 74 6 76 243 2 157 1.29e-37 130
MS.gene052017.t1 AT3G59470 35.586 222 124 7 31 244 32 242 2.92e-35 126
MS.gene052017.t1 AT3G59470 35.586 222 124 7 31 244 32 242 2.92e-35 126
MS.gene052017.t1 AT3G59470 35.586 222 124 7 31 244 44 254 3.71e-35 126
MS.gene052017.t1 AT4G12850 34.783 184 104 4 60 243 10 177 3.84e-33 119
MS.gene052017.t1 AT4G38180 44.262 122 67 1 50 170 62 183 1.11e-23 100
MS.gene052017.t1 AT4G12850 39.048 105 59 1 60 164 10 109 4.66e-22 89.0
MS.gene052017.t1 AT4G12850 39.048 105 59 1 60 164 10 109 4.98e-22 89.0
MS.gene052017.t1 AT5G18960 42.453 106 46 1 59 164 41 131 5.04e-21 92.4
MS.gene052017.t1 AT5G18960 35.238 105 53 1 60 164 210 299 2.54e-15 75.5
MS.gene052017.t1 AT5G18960 42.453 106 46 1 59 164 41 131 5.04e-21 92.4
MS.gene052017.t1 AT5G18960 35.238 105 53 1 60 164 210 299 2.54e-15 75.5
MS.gene052017.t1 AT3G06250 41.509 106 47 1 59 164 26 116 2.48e-20 90.5
MS.gene052017.t1 AT3G06250 36.066 122 59 2 43 164 176 278 9.89e-17 79.7
MS.gene052017.t1 AT3G06250 41.509 106 47 1 59 164 26 116 2.48e-20 90.5
MS.gene052017.t1 AT3G06250 36.066 122 59 2 43 164 176 278 9.89e-17 79.7
MS.gene052017.t1 AT3G06250 41.509 106 47 1 59 164 26 116 2.48e-20 90.5
MS.gene052017.t1 AT3G06250 36.066 122 59 2 43 164 176 278 9.89e-17 79.7
MS.gene052017.t1 AT2G27110 37.008 127 62 3 41 166 28 137 5.14e-15 74.7
MS.gene052017.t1 AT2G27110 37.008 127 62 3 41 166 28 137 5.14e-15 74.7
MS.gene052017.t1 AT3G22170 31.707 123 73 1 59 170 68 190 1.29e-14 73.6
MS.gene052017.t1 AT3G22170 31.707 123 73 1 59 170 68 190 1.29e-14 73.6
MS.gene052017.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.43e-13 70.5
MS.gene052017.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.45e-13 70.5
MS.gene052017.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.45e-13 70.5
MS.gene052017.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.45e-13 70.5
MS.gene052017.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.48e-13 70.5
MS.gene052017.t1 AT2G32250 34.653 101 61 1 64 164 41 136 1.48e-13 70.5
MS.gene052017.t1 AT4G19990 31.579 114 67 1 64 166 38 151 7.22e-13 68.2
MS.gene052017.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.03e-13 68.2
MS.gene052017.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.03e-13 68.2
MS.gene052017.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.33e-13 68.2
MS.gene052017.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.33e-13 68.2
MS.gene052017.t1 AT4G19990 31.579 114 67 1 64 166 24 137 8.57e-13 68.2
MS.gene052017.t1 AT2G32250 34.000 100 61 1 65 164 1 95 1.21e-12 67.8
MS.gene052017.t1 AT2G32250 34.000 100 61 1 65 164 1 95 1.22e-12 67.8
MS.gene052017.t1 AT1G76320 32.673 101 63 1 65 165 1 96 2.51e-12 66.6
MS.gene052017.t1 AT1G76320 30.159 126 82 2 65 190 1 120 2.66e-12 66.6
MS.gene052017.t1 AT1G76320 30.159 126 82 2 65 190 1 120 2.66e-12 66.6
MS.gene052017.t1 AT1G76320 30.159 126 82 2 65 190 1 120 2.99e-12 66.6
MS.gene052017.t1 AT1G80010 33.654 104 63 1 61 164 95 192 4.26e-12 65.9
MS.gene052017.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene052017.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene052017.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene052017.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene052017.t1 AT4G15090 31.776 107 71 1 59 165 49 153 4.84e-12 65.9
MS.gene052017.t1 AT1G52520 32.692 104 64 1 61 164 85 182 7.80e-12 65.1

Find 62 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATAAGGTTGGCTTTAC+GGG 0.249268 2.2:-3940373 MS.gene052017:CDS
TGTATGTTCCTTCACAAATT+TGG 0.258446 2.2:+3940329 None:intergenic
CCATGATAAGGTTGGCTTTA+CGG 0.274945 2.2:-3940374 MS.gene052017:CDS
TCTCGGGTGGCTGGTGGAGG+AGG 0.303392 2.2:+3940408 None:intergenic
CCTCAATCTCTCTGATATTT+CGG 0.319830 2.2:+3939291 None:intergenic
AAATACAATTCTGAACGATT+TGG 0.340360 2.2:-3939357 MS.gene052017:CDS
TTACTCGCGAACGGCGGTTA+TGG 0.347299 2.2:+3940517 None:intergenic
CGAGTAAATAACAAGCTAAT+TGG 0.351629 2.2:-3940501 MS.gene052017:CDS
TCATGGCTTGACAACCTTCT+CGG 0.378795 2.2:+3940391 None:intergenic
CCTTCTCGGGTGGCTGGTGG+AGG 0.389419 2.2:+3940405 None:intergenic
GAAGTTCCATGGCGAGGATC+CGG 0.395395 2.2:-3940285 MS.gene052017:CDS
CTCACTAACCAAGTGCTTCC+CGG 0.404686 2.2:+3940266 None:intergenic
CATGGCTTGACAACCTTCTC+GGG 0.412731 2.2:+3940392 None:intergenic
TGACAACCTTCTCGGGTGGC+TGG 0.424878 2.2:+3940399 None:intergenic
TCCTCGCCATGGAACTTCAC+TGG 0.443266 2.2:+3940290 None:intergenic
AGGATGATGGATCACCAATC+TGG 0.443741 2.2:-3940792 MS.gene052017:CDS
GACACATTAGAACCATTCAT+AGG 0.454194 2.2:-3940621 MS.gene052017:CDS
TGTCACAGGACGAGAAAGAT+AGG 0.454669 2.2:-3939460 MS.gene052017:intron
AATCAAATCCCTGGCCAGAT+TGG 0.455766 2.2:+3940778 None:intergenic
CGAACAACCTGTAGCAGATT+CGG 0.459103 2.2:-3940643 MS.gene052017:CDS
CCGAAATATCAGAGAGATTG+AGG 0.462386 2.2:-3939291 MS.gene052017:CDS
CTCGGGTGGCTGGTGGAGGA+GGG 0.466145 2.2:+3940409 None:intergenic
GATGGATCACCAATCTGGCC+AGG 0.492007 2.2:-3940787 MS.gene052017:CDS
CAACCTTCTCGGGTGGCTGG+TGG 0.497367 2.2:+3940402 None:intergenic
AGGTTGGCTTTACGGGATGA+AGG 0.498475 2.2:-3940366 MS.gene052017:CDS
AAGTTCCATGGCGAGGATCC+GGG 0.502060 2.2:-3940284 MS.gene052017:CDS
AAGGTTGTCAAGCCATGATA+AGG 0.504631 2.2:-3940386 MS.gene052017:CDS
ACGAAAGGCAAAAGTACAAA+AGG 0.509895 2.2:-3939406 MS.gene052017:CDS
CTTTACGGGATGAAGGGAAG+TGG 0.510166 2.2:-3940359 MS.gene052017:CDS
CCTCGCCATGGAACTTCACT+GGG 0.518653 2.2:+3940291 None:intergenic
AATGTGTCCGAATCTGCTAC+AGG 0.521451 2.2:+3940636 None:intergenic
AACAATTTAGAATTATGTAC+AGG 0.522470 2.2:-3940684 MS.gene052017:CDS
CGCTCGAGTTGCACAACGAA+AGG 0.523644 2.2:-3939421 MS.gene052017:CDS
CCGTAAAGCCAACCTTATCA+TGG 0.524530 2.2:+3940374 None:intergenic
AATGGCCATGGTAGGAGGAT+AGG 0.534923 2.2:-3940558 MS.gene052017:CDS
GCGAGGATCCGGGAAGCACT+TGG 0.541373 2.2:-3940274 MS.gene052017:CDS
CCTCCACCAGCCACCCGAGA+AGG 0.543028 2.2:-3940405 MS.gene052017:CDS
TGCTTCCCGGATCCTCGCCA+TGG 0.557183 2.2:+3940279 None:intergenic
TTTACGGGATGAAGGGAAGT+GGG 0.561477 2.2:-3940358 MS.gene052017:CDS
GGCCAGGGATTTGATTCAGA+TGG 0.568231 2.2:-3940771 MS.gene052017:CDS
GGTTGGCTTTACGGGATGAA+GGG 0.571058 2.2:-3940365 MS.gene052017:CDS
TAGGCATGCAGTTCAACTCA+AGG 0.572467 2.2:-3940602 MS.gene052017:CDS
TCTACAATGGCCATGGTAGG+AGG 0.572483 2.2:-3940563 MS.gene052017:CDS
TACCATCTGAATCAAATCCC+TGG 0.581654 2.2:+3940769 None:intergenic
GCTTGTTATTTACTCGCGAA+CGG 0.583871 2.2:+3940508 None:intergenic
GGAGGATAGGATTTACAGTA+CGG 0.583900 2.2:-3940545 MS.gene052017:CDS
AGAGAATTCTACAATGGCCA+TGG 0.595180 2.2:-3940570 MS.gene052017:CDS
TTGTCAAGCCATGATAAGGT+TGG 0.599156 2.2:-3940382 MS.gene052017:CDS
CCCAGTGAAGTTCCATGGCG+AGG 0.602502 2.2:-3940291 MS.gene052017:CDS
TAAATCCTATCCTCCTACCA+TGG 0.603101 2.2:+3940553 None:intergenic
TTGAACTGCATGCCTATGAA+TGG 0.604946 2.2:+3940609 None:intergenic
TGAGTCCCAGTGAAGTTCCA+TGG 0.607822 2.2:-3940296 MS.gene052017:CDS
ATGGATCACCAATCTGGCCA+GGG 0.613451 2.2:-3940786 MS.gene052017:CDS
AATTCTACAATGGCCATGGT+AGG 0.613869 2.2:-3940566 MS.gene052017:CDS
GGCTTGACAACCTTCTCGGG+TGG 0.617601 2.2:+3940395 None:intergenic
GGTTGTCACCAAATTTGTGA+AGG 0.624088 2.2:-3940337 MS.gene052017:CDS
CGGGAAGCACTTGGTTAGTG+AGG 0.627824 2.2:-3940265 MS.gene052017:intron
AGGCATGCAGTTCAACTCAA+GGG 0.634792 2.2:-3940601 MS.gene052017:CDS
TGTTATTTACTCGCGAACGG+CGG 0.635708 2.2:+3940511 None:intergenic
GCAACTCGAGCGATAGCTCA+CGG 0.642945 2.2:+3939432 None:intergenic
GAAGCAAGAGAATTCTACAA+TGG 0.657489 2.2:-3940576 MS.gene052017:CDS
CATGCAGTTCAACTCAAGGG+AGG 0.700261 2.2:-3940598 MS.gene052017:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GACTATATTATGTATATTCT+TGG + chr2.2:3940347-3940366 None:intergenic 20.0%
!! TATCAAAATACAGATTCTTA+TGG - chr2.2:3940084-3940103 MS.gene052017:intron 20.0%
!!! AACAATTTAGAATTATGTAC+AGG - chr2.2:3939367-3939386 MS.gene052017:CDS 20.0%
!!! TTTTAGAATATCTTATCTCT+GGG - chr2.2:3940110-3940129 MS.gene052017:intron 20.0%
! AAATACAATTCTGAACGATT+TGG - chr2.2:3940694-3940713 MS.gene052017:CDS 25.0%
! GAAGAAAAATCAGATTTAGA+TGG - chr2.2:3940782-3940801 MS.gene052017:CDS 25.0%
! TAAACTATAAAGTGCACTAT+TGG + chr2.2:3939821-3939840 None:intergenic 25.0%
!! AGGATTGTATTAACTCTTTT+AGG - chr2.2:3940423-3940442 MS.gene052017:CDS 25.0%
!! GGATTGTATTAACTCTTTTA+GGG - chr2.2:3940424-3940443 MS.gene052017:CDS 25.0%
!!! CCTGTTTGAGATTTTTTTTA+GGG + chr2.2:3940406-3940425 None:intergenic 25.0%
!!! CTGTTTGAGATTTTTTTTAG+GGG + chr2.2:3940405-3940424 None:intergenic 25.0%
!!! CTTTTAGAATATCTTATCTC+TGG - chr2.2:3940109-3940128 MS.gene052017:intron 25.0%
!!! TCCTGTTTGAGATTTTTTTT+AGG + chr2.2:3940407-3940426 None:intergenic 25.0%
!!! TGTTTGAGATTTTTTTTAGG+GGG + chr2.2:3940404-3940423 None:intergenic 25.0%
!!! TTCTAGAATACCTTATCTAT+GGG - chr2.2:3940013-3940032 MS.gene052017:intron 25.0%
AAAAAGGAACAAACAGAACA+TGG + chr2.2:3940543-3940562 None:intergenic 30.0%
AAAAATCAGATTTAGATGGC+AGG - chr2.2:3940786-3940805 MS.gene052017:CDS 30.0%
AAATCAACAAGACAAGTTTG+AGG + chr2.2:3940570-3940589 None:intergenic 30.0%
ATTAAAAGTCCCATCTCAAT+CGG - chr2.2:3940448-3940467 MS.gene052017:CDS 30.0%
CCCTAAAAAAAATCTCAAAC+AGG - chr2.2:3940403-3940422 MS.gene052017:CDS 30.0%
CGAGTAAATAACAAGCTAAT+TGG - chr2.2:3939550-3939569 MS.gene052017:intron 30.0%
GAAAATTGTGAGTGAAAGTT+TGG + chr2.2:3940138-3940157 None:intergenic 30.0%
GTATAGTTCAGTTCTTTGTT+AGG - chr2.2:3940508-3940527 MS.gene052017:CDS 30.0%
TAGCAAAATACAGATTCTCA+TGG - chr2.2:3939987-3940006 MS.gene052017:intron 30.0%
TATGTATACCTGATCAAACT+TGG - chr2.2:3939852-3939871 MS.gene052017:intron 30.0%
TGTATGTTCCTTCACAAATT+TGG + chr2.2:3939725-3939744 None:intergenic 30.0%
! ACAAGTTTGAGGTAGAAAAA+AGG + chr2.2:3940559-3940578 None:intergenic 30.0%
! ATTCTTGGTGTAATATCGAT+AGG + chr2.2:3940332-3940351 None:intergenic 30.0%
! TAGCAAGAGAATTTAAGACA+TGG + chr2.2:3940284-3940303 None:intergenic 30.0%
!! CTTCTAGAATACCTTATCTA+TGG - chr2.2:3940012-3940031 MS.gene052017:intron 30.0%
CCTCAATCTCTCTGATATTT+CGG + chr2.2:3940763-3940782 None:intergenic 35.0%
GAAAAATGTGAGTGAAGGTT+TGG + chr2.2:3940041-3940060 None:intergenic 35.0%
GAAGCAAGAGAATTCTACAA+TGG - chr2.2:3939475-3939494 MS.gene052017:intron 35.0%
GACACATTAGAACCATTCAT+AGG - chr2.2:3939430-3939449 MS.gene052017:CDS 35.0%
TTACATGCAGATGTTGGATT+AGG - chr2.2:3940471-3940490 MS.gene052017:CDS 35.0%
TTCCACCTAAGATAGAATGA+GGG - chr2.2:3940306-3940325 MS.gene052017:CDS 35.0%
TTTCCACCTAAGATAGAATG+AGG - chr2.2:3940305-3940324 MS.gene052017:CDS 35.0%
! ACGAAAGGCAAAAGTACAAA+AGG - chr2.2:3940645-3940664 MS.gene052017:CDS 35.0%
! GATTTTGTCTGCTCAAAAGA+AGG - chr2.2:3939577-3939596 MS.gene052017:intron 35.0%
!! GTCAGTCTCACTTTTAAATC+TGG + chr2.2:3939878-3939897 None:intergenic 35.0%
!!! TTGAGATTTTTTTTAGGGGG+TGG + chr2.2:3940401-3940420 None:intergenic 35.0%
AAATCTGGCCAAGTTTGATC+AGG + chr2.2:3939863-3939882 None:intergenic 40.0%
AAGGTTGTCAAGCCATGATA+AGG - chr2.2:3939665-3939684 MS.gene052017:intron 40.0%
AATCGGTTACATGCAGATGT+TGG - chr2.2:3940465-3940484 MS.gene052017:CDS 40.0%
AATTCTACAATGGCCATGGT+AGG - chr2.2:3939485-3939504 MS.gene052017:intron 40.0%
AGAGAATTCTACAATGGCCA+TGG - chr2.2:3939481-3939500 MS.gene052017:intron 40.0%
AGATTGACAACAGTATCAGC+AGG - chr2.2:3939933-3939952 MS.gene052017:intron 40.0%
ATAGGCCCTCATTCTATCTT+AGG + chr2.2:3940314-3940333 None:intergenic 40.0%
ATCAGTGAGGGACTTAGAAA+AGG + chr2.2:3940181-3940200 None:intergenic 40.0%
CCGAAATATCAGAGAGATTG+AGG - chr2.2:3940760-3940779 MS.gene052017:CDS 40.0%
GGAGGATAGGATTTACAGTA+CGG - chr2.2:3939506-3939525 MS.gene052017:intron 40.0%
GGTTGTCACCAAATTTGTGA+AGG - chr2.2:3939714-3939733 MS.gene052017:intron 40.0%
GTCAATCTAATATCAGCAGC+AGG + chr2.2:3939921-3939940 None:intergenic 40.0%
TAAATCCTATCCTCCTACCA+TGG + chr2.2:3939501-3939520 None:intergenic 40.0%
TACCATCTGAATCAAATCCC+TGG + chr2.2:3939285-3939304 None:intergenic 40.0%
TCAGTGAGGGACTTAGAAAA+GGG + chr2.2:3940180-3940199 None:intergenic 40.0%
TGATGCAAATGGAAGGGAAA+GGG + chr2.2:3940214-3940233 None:intergenic 40.0%
TGCATGTAACCGATTGAGAT+GGG + chr2.2:3940460-3940479 None:intergenic 40.0%
TTGAACTGCATGCCTATGAA+TGG + chr2.2:3939445-3939464 None:intergenic 40.0%
TTGTCAAGCCATGATAAGGT+TGG - chr2.2:3939669-3939688 MS.gene052017:intron 40.0%
! AGGTTTGGAACCCATAGATA+AGG + chr2.2:3940026-3940045 None:intergenic 40.0%
! GGATTTTGTTGGATCAGTGA+GGG + chr2.2:3940193-3940212 None:intergenic 40.0%
! TTCGAGTTCATCATGTAGAG+CGG + chr2.2:3940064-3940083 None:intergenic 40.0%
!! CATGATAAGGTTGGCTTTAC+GGG - chr2.2:3939678-3939697 MS.gene052017:intron 40.0%
!! CCATGATAAGGTTGGCTTTA+CGG - chr2.2:3939677-3939696 MS.gene052017:intron 40.0%
!! GCTTGTTATTTACTCGCGAA+CGG + chr2.2:3939546-3939565 None:intergenic 40.0%
!! GTCTTGTTGATTTGTGTCAC+AGG - chr2.2:3940577-3940596 MS.gene052017:CDS 40.0%
!!! AGATTTTTTTTAGGGGGTGG+AGG + chr2.2:3940398-3940417 None:intergenic 40.0%
AACGACGTGATGCAAATGGA+AGG + chr2.2:3940221-3940240 None:intergenic 45.0%
AATATCAGCAGCAGGAGAAG+AGG + chr2.2:3939913-3939932 None:intergenic 45.0%
AATCAAATCCCTGGCCAGAT+TGG + chr2.2:3939276-3939295 None:intergenic 45.0%
AATGTGTCCGAATCTGCTAC+AGG + chr2.2:3939418-3939437 None:intergenic 45.0%
ACGACGTGATGCAAATGGAA+GGG + chr2.2:3940220-3940239 None:intergenic 45.0%
AGCGGGAAAAATGTGAGTGA+AGG + chr2.2:3940046-3940065 None:intergenic 45.0%
AGGCATGCAGTTCAACTCAA+GGG - chr2.2:3939450-3939469 MS.gene052017:CDS 45.0%
ATGGCAGCATGAGACACATT+GGG + chr2.2:3940265-3940284 None:intergenic 45.0%
CCGTAAAGCCAACCTTATCA+TGG + chr2.2:3939680-3939699 None:intergenic 45.0%
CGAACAACCTGTAGCAGATT+CGG - chr2.2:3939408-3939427 MS.gene052017:CDS 45.0%
CTGCATGTAACCGATTGAGA+TGG + chr2.2:3940461-3940480 None:intergenic 45.0%
GTGATGCAAATGGAAGGGAA+AGG + chr2.2:3940215-3940234 None:intergenic 45.0%
TAGGCATGCAGTTCAACTCA+AGG - chr2.2:3939449-3939468 MS.gene052017:CDS 45.0%
TCATGGCTTGACAACCTTCT+CGG + chr2.2:3939663-3939682 None:intergenic 45.0%
TCCATTTGCATCACGTCGTT+AGG - chr2.2:3940221-3940240 MS.gene052017:intron 45.0%
TCCTAACGACGTGATGCAAA+TGG + chr2.2:3940225-3940244 None:intergenic 45.0%
TCGAGTTCATCATGTAGAGC+GGG + chr2.2:3940063-3940082 None:intergenic 45.0%
TGTCACAGGACGAGAAAGAT+AGG - chr2.2:3940591-3940610 MS.gene052017:CDS 45.0%
TTTACGGGATGAAGGGAAGT+GGG - chr2.2:3939693-3939712 MS.gene052017:intron 45.0%
! AAAAGGGTGTGTGGAAAGTG+AGG + chr2.2:3940164-3940183 None:intergenic 45.0%
! AGGACTAGAGGTAGCTTTTC+AGG + chr2.2:3940378-3940397 None:intergenic 45.0%
! GGAAGGGAAAGGGATTTTGT+TGG + chr2.2:3940204-3940223 None:intergenic 45.0%
! GGGATTTTGTTGGATCAGTG+AGG + chr2.2:3940194-3940213 None:intergenic 45.0%
! TGTTATTTACTCGCGAACGG+CGG + chr2.2:3939543-3939562 None:intergenic 45.0%
AATGGCCATGGTAGGAGGAT+AGG - chr2.2:3939493-3939512 MS.gene052017:intron 50.0%
ATGGATCACCAATCTGGCCA+GGG - chr2.2:3939265-3939284 MS.gene052017:CDS 50.0%
CATGCAGTTCAACTCAAGGG+AGG - chr2.2:3939453-3939472 MS.gene052017:CDS 50.0%
CATGGCAGCATGAGACACAT+TGG + chr2.2:3940266-3940285 None:intergenic 50.0%
CATGGCTTGACAACCTTCTC+GGG + chr2.2:3939662-3939681 None:intergenic 50.0%
CTCACTAACCAAGTGCTTCC+CGG + chr2.2:3939788-3939807 None:intergenic 50.0%
CTTTACGGGATGAAGGGAAG+TGG - chr2.2:3939692-3939711 MS.gene052017:intron 50.0%
GGCCAGGGATTTGATTCAGA+TGG - chr2.2:3939280-3939299 MS.gene052017:CDS 50.0%
GGCCCTCATTCTATCTTAGG+TGG + chr2.2:3940311-3940330 None:intergenic 50.0%
TCTACAATGGCCATGGTAGG+AGG - chr2.2:3939488-3939507 MS.gene052017:intron 50.0%
TGAGTCCCAGTGAAGTTCCA+TGG - chr2.2:3939755-3939774 MS.gene052017:intron 50.0%
! GGGACTTAGAAAAGGGTGTG+TGG + chr2.2:3940173-3940192 None:intergenic 50.0%
!! AGGTTGGCTTTACGGGATGA+AGG - chr2.2:3939685-3939704 MS.gene052017:intron 50.0%
!! GGTTGGCTTTACGGGATGAA+GGG - chr2.2:3939686-3939705 MS.gene052017:intron 50.0%
AAGTTCCATGGCGAGGATCC+GGG - chr2.2:3939767-3939786 MS.gene052017:intron 55.0%
CCTCGCCATGGAACTTCACT+GGG + chr2.2:3939763-3939782 None:intergenic 55.0%
GAAGTTCCATGGCGAGGATC+CGG - chr2.2:3939766-3939785 MS.gene052017:intron 55.0%
GATGGATCACCAATCTGGCC+AGG - chr2.2:3939264-3939283 MS.gene052017:CDS 55.0%
GCAACTCGAGCGATAGCTCA+CGG + chr2.2:3940622-3940641 None:intergenic 55.0%
TCCTCGCCATGGAACTTCAC+TGG + chr2.2:3939764-3939783 None:intergenic 55.0%
TTACTCGCGAACGGCGGTTA+TGG + chr2.2:3939537-3939556 None:intergenic 55.0%
TTTAGGGGGTGGAGGACTAG+AGG + chr2.2:3940390-3940409 None:intergenic 55.0%
! CGCTCGAGTTGCACAACGAA+AGG - chr2.2:3940630-3940649 MS.gene052017:CDS 55.0%
!! CGGGAAGCACTTGGTTAGTG+AGG - chr2.2:3939786-3939805 MS.gene052017:intron 55.0%
CCCAGTGAAGTTCCATGGCG+AGG - chr2.2:3939760-3939779 MS.gene052017:intron 60.0%
GGCTTGACAACCTTCTCGGG+TGG + chr2.2:3939659-3939678 None:intergenic 60.0%
GGGTGTGTGGAAAGTGAGGC+TGG + chr2.2:3940160-3940179 None:intergenic 60.0%
TGACAACCTTCTCGGGTGGC+TGG + chr2.2:3939655-3939674 None:intergenic 60.0%
CAACCTTCTCGGGTGGCTGG+TGG + chr2.2:3939652-3939671 None:intergenic 65.0%
TGCTTCCCGGATCCTCGCCA+TGG + chr2.2:3939775-3939794 None:intergenic 65.0%
!! GCGAGGATCCGGGAAGCACT+TGG - chr2.2:3939777-3939796 MS.gene052017:intron 65.0%
CCTCCACCAGCCACCCGAGA+AGG - chr2.2:3939646-3939665 MS.gene052017:intron 70.0%
CCTTCTCGGGTGGCTGGTGG+AGG + chr2.2:3939649-3939668 None:intergenic 70.0%
CTCGGGTGGCTGGTGGAGGA+GGG + chr2.2:3939645-3939664 None:intergenic 70.0%
TCTCGGGTGGCTGGTGGAGG+AGG + chr2.2:3939646-3939665 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 3939262 3940811 3939262 ID=MS.gene052017
chr2.2 mRNA 3939262 3940811 3939262 ID=MS.gene052017.t1;Parent=MS.gene052017
chr2.2 exon 3940266 3940811 3940266 ID=MS.gene052017.t1.exon1;Parent=MS.gene052017.t1
chr2.2 CDS 3940266 3940811 3940266 ID=cds.MS.gene052017.t1;Parent=MS.gene052017.t1
chr2.2 exon 3939262 3939474 3939262 ID=MS.gene052017.t1.exon2;Parent=MS.gene052017.t1
chr2.2 CDS 3939262 3939474 3939262 ID=cds.MS.gene052017.t1;Parent=MS.gene052017.t1
Gene Sequence

>MS.gene052017

ATGATGGATCACCAATCTGGCCAGGGATTTGATTCAGATGGTAGTGACTTTGATGTGCTAGCAGAAATCAGTGAGAAACACGAGAAAGTAGAAGATGAGTTTACAAACAATTTAGAATTATGTACAGGCGAAGATGAAAAGTTTGTCGAACAACCTGTAGCAGATTCGGACACATTAGAACCATTCATAGGCATGCAGTTCAACTCAAGGGAGGAAGCAAGAGAATTCTACAATGGCCATGGTAGGAGGATAGGATTTACAGTACGGATACACCATAACCGCCGTTCGCGAGTAAATAACAAGCTAATTGGTCAAGATTTTGTCTGCTCAAAAGAAGGTTTTCGTGCAAAAAAGTATGTGCACAGAAAAGATAGAGTGCTCCCTCCTCCACCAGCCACCCGAGAAGGTTGTCAAGCCATGATAAGGTTGGCTTTACGGGATGAAGGGAAGTGGGTTGTCACCAAATTTGTGAAGGAACATACACATAAACTGATGAGTCCCAGTGAAGTTCCATGGCGAGGATCCGGGAAGCACTTGGTTAGTGAGGTACGTTTCTCCAATAGTGCACTTTATAGTTTAAACTAACAGTTTATGTATACCTGATCAAACTTGGCCAGATTTAAAAGTGAGACTGACTAATGCATTATACCTCTTCTCCTGCTGCTGATATTAGATTGACAACAGTATCAGCAGGATTAAACTACACTGAACATGATGAACTCGAATAGCAAAATACAGATTCTCATGGAGCTTCTAGAATACCTTATCTATGGGTTCCAAACCTTCACTCACATTTTTCCCGCTCTACATGATGAACTCGAATATCAAAATACAGATTCTTATGGAGCTTTTAGAATATCTTATCTCTGGGTTCCAAACTTTCACTCACAATTTTCCAGCCTCACTTTCCACACACCCTTTTCTAAGTCCCTCACTGATCCAACAAAATCCCTTTCCCTTCCATTTGCATCACGTCGTTAGGAAGTTAGTTATCTGAAGTCCCAATGTGTCTCATGCTGCCATGTCTTAAATTCTCTTGCTATTTTCCACCTAAGATAGAATGAGGGCCTATCGATATTACACCAAGAATATACATAATATAGTCAATTATAGCCTGAAAAGCTACCTCTAGTCCTCCACCCCCTAAAAAAAATCTCAAACAGGATTGTATTAACTCTTTTAGGGCATTAAAAGTCCCATCTCAATCGGTTACATGCAGATGTTGGATTAGGATATTTTGATTTGTGTATAGTTCAGTTCTTTGTTAGGATTGAATGTCCATGTTCTGTTTGTTCCTTTTTTCTACCTCAAACTTGTCTTGTTGATTTGTGTCACAGGACGAGAAAGATAGGAGAATCCGTGAGCTATCGCTCGAGTTGCACAACGAAAGGCAAAAGTACAAAAGGCGTTGTGCAGCATATGAAGAACAGTTAAATACAATTCTGAACGATTTGGAAAAGCACACAGAACATATCTCTAAAAAAGTTGCTGATGTAGTCCGAAATATCAGAGAGATTGAGGAAGAAAAATCAGATTTAGATGGCAGGTAA
Protein sequence

>MS.gene052017.t1

MMDHQSGQGFDSDGSDFDVLAEISEKHEKVEDEFTNNLELCTGEDEKFVEQPVADSDTLEPFIGMQFNSREEAREFYNGHGRRIGFTVRIHHNRRSRVNNKLIGQDFVCSKEGFRAKKYVHRKDRVLPPPPATREGCQAMIRLALRDEGKWVVTKFVKEHTHKLMSPSEVPWRGSGKHLVSEDEKDRRIRELSLELHNERQKYKRRCAAYEEQLNTILNDLEKHTEHISKKVADVVRNIREIEEEKSDLDGR