AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052096

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052096.t1 MTR_2g097350 91.277 642 56 0 1 642 1 642 0.0 1161
MS.gene052096.t1 MTR_2g097390 83.717 651 89 4 1 642 1 643 0.0 1071
MS.gene052096.t1 MTR_2g097310 83.436 652 86 6 1 642 1 640 0.0 1069
MS.gene052096.t1 MTR_2g097380 76.364 495 66 5 155 642 113 563 0.0 765
MS.gene052096.t1 MTR_4g064160 60.360 444 163 6 209 642 246 686 0.0 554
MS.gene052096.t1 MTR_4g064120 42.345 614 324 13 46 642 28 628 1.83e-155 462
MS.gene052096.t1 MTR_2g097410 51.902 447 197 9 209 642 302 743 2.11e-154 463
MS.gene052096.t1 MTR_4g064180 41.329 617 326 12 46 642 28 628 3.46e-151 451
MS.gene052096.t1 MTR_4g064150 40.888 653 326 17 46 642 87 735 1.20e-150 453
MS.gene052096.t1 MTR_7g062120 51.938 387 180 3 260 642 358 742 1.38e-150 453
MS.gene052096.t1 MTR_2g097467 37.859 626 336 13 50 642 51 656 3.25e-143 431
MS.gene052096.t1 MTR_4g064200 46.204 461 221 7 202 642 199 652 5.58e-140 423
MS.gene052096.t1 MTR_2g097473 38.264 622 339 12 50 642 51 656 5.82e-139 421
MS.gene052096.t1 MTR_2g097463 38.042 623 332 14 54 642 55 657 8.10e-139 420
MS.gene052096.t1 MTR_5g097480 31.467 375 248 4 270 639 173 543 1.42e-61 214
MS.gene052096.t1 MTR_3g056110 28.597 549 332 16 123 639 21 541 1.66e-61 214
MS.gene052096.t1 MTR_2g082090 31.467 375 248 4 270 639 208 578 3.28e-59 209
MS.gene052096.t1 MTR_2g026250 31.332 383 252 5 265 639 216 595 1.93e-58 207
MS.gene052096.t1 MTR_3g089055 32.888 374 240 6 272 639 196 564 2.11e-58 206
MS.gene052096.t1 MTR_3g089055 32.888 374 240 6 272 639 201 569 2.47e-58 206
MS.gene052096.t1 MTR_4g133660 30.239 377 255 4 269 640 170 543 5.50e-58 204
MS.gene052096.t1 MTR_1g029420 31.452 372 248 3 272 639 223 591 4.53e-56 200
MS.gene052096.t1 MTR_5g094450 30.789 380 248 9 270 639 157 531 2.61e-54 194
MS.gene052096.t1 MTR_7g027190 30.612 392 237 7 269 639 295 672 3.70e-53 194
MS.gene052096.t1 MTR_8g442410 29.897 388 242 10 269 639 150 524 7.07e-49 179
MS.gene052096.t1 MTR_3g065980 31.122 392 240 7 264 639 162 539 1.03e-48 179
MS.gene052096.t1 MTR_4g104020 28.272 382 253 8 271 639 146 519 1.94e-48 177
MS.gene052096.t1 MTR_7g074650 32.055 365 234 8 278 641 442 793 9.48e-48 180
MS.gene052096.t1 MTR_5g009080 26.364 440 260 8 260 639 42 477 5.68e-41 156
MS.gene052096.t1 MTR_4g102790 26.110 383 265 8 270 641 288 663 5.91e-41 159
MS.gene052096.t1 MTR_4g076140 26.316 437 257 9 263 639 37 468 2.46e-39 151
MS.gene052096.t1 MTR_3g022830 26.506 415 250 7 275 639 26 435 2.56e-39 150
MS.gene052096.t1 MTR_4g097080 26.316 380 264 7 272 641 131 504 4.99e-38 148
MS.gene052096.t1 MTR_4g077760 28.684 380 244 10 271 639 191 554 1.24e-36 145
MS.gene052096.t1 MTR_2g089100 28.293 410 243 10 271 642 49 445 5.87e-36 141
MS.gene052096.t1 MTR_2g034260 24.615 390 270 8 266 639 202 583 3.18e-34 138
MS.gene052096.t1 MTR_5g015490 25.654 382 266 9 274 641 114 491 1.67e-32 132
MS.gene052096.t1 MTR_4g076020 28.455 369 235 10 278 642 88 431 2.17e-32 130
MS.gene052096.t1 MTR_1g069725 27.968 379 251 11 266 639 101 462 5.33e-32 130
MS.gene052096.t1 MTR_2g034250 25.641 390 267 8 266 639 202 584 1.11e-31 130
MS.gene052096.t1 MTR_2g034280 24.352 386 274 7 266 639 195 574 9.56e-30 124
MS.gene052096.t1 MTR_1g096030 28.715 397 234 17 274 639 80 458 2.17e-28 119
MS.gene052096.t1 MTR_7g057230 27.178 287 198 5 359 639 375 656 2.38e-28 120
MS.gene052096.t1 MTR_7g109580 25.654 382 261 10 269 639 184 553 2.74e-27 117
MS.gene052096.t1 MTR_6g047750 26.260 377 255 8 275 639 296 661 8.03e-27 116
MS.gene052096.t1 MTR_2g099110 27.436 390 250 11 274 641 74 452 1.37e-24 107
MS.gene052096.t1 MTR_5g058860 26.531 392 248 16 274 639 119 496 2.95e-23 104
MS.gene052096.t1 MTR_4g095500 22.170 424 269 10 271 642 56 470 7.73e-22 99.4
MS.gene052096.t1 MTR_3g072710 26.173 405 245 15 269 639 115 499 1.02e-21 99.4
MS.gene052096.t1 MTR_1g086970 23.469 392 264 13 269 639 101 477 3.94e-21 97.4
MS.gene052096.t1 MTR_8g093070 23.678 397 272 13 257 642 122 498 8.41e-18 87.4
MS.gene052096.t1 MTR_4g026485 23.200 375 257 12 275 639 270 623 1.36e-14 77.4
MS.gene052096.t1 MTR_2g097370 93.750 32 2 0 62 93 1 32 2.41e-14 68.9
MS.gene052096.t1 MTR_0092s0100 23.947 380 249 12 275 639 376 730 4.35e-14 76.3
MS.gene052096.t1 MTR_0092s0100 23.947 380 249 12 275 639 373 727 4.65e-14 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052096.t1 AT2G29060 43.226 620 304 11 50 641 57 656 6.71e-171 503
MS.gene052096.t1 AT2G29060 42.163 638 322 11 50 641 57 693 1.77e-168 498
MS.gene052096.t1 AT1G07530 51.183 465 199 7 199 642 310 767 1.04e-165 493
MS.gene052096.t1 AT1G07520 48.322 447 215 8 207 642 90 531 2.28e-145 432
MS.gene052096.t1 AT1G07520 48.322 447 215 8 207 642 90 531 2.28e-145 432
MS.gene052096.t1 AT2G29065 41.100 618 318 15 50 642 34 630 1.75e-144 434
MS.gene052096.t1 AT1G07520 48.322 447 215 8 207 642 254 695 4.24e-144 435
MS.gene052096.t1 AT1G07520 48.322 447 215 8 207 642 255 696 4.65e-144 435
MS.gene052096.t1 AT2G37650 49.479 384 186 4 262 641 323 702 7.03e-131 401
MS.gene052096.t1 AT2G37650 49.479 384 186 4 262 641 335 714 9.40e-131 401
MS.gene052096.t1 AT5G59450 37.274 609 302 14 49 642 57 600 1.32e-121 374
MS.gene052096.t1 AT3G46600 43.192 426 231 6 221 642 163 581 8.99e-118 363
MS.gene052096.t1 AT3G46600 43.192 426 231 6 221 642 131 549 1.01e-117 362
MS.gene052096.t1 AT3G46600 44.947 376 200 4 271 642 1 373 4.61e-113 344
MS.gene052096.t1 AT5G48150 32.653 392 256 5 253 639 101 489 1.77e-63 218
MS.gene052096.t1 AT5G48150 32.653 392 256 5 253 639 101 489 1.77e-63 218
MS.gene052096.t1 AT5G48150 32.653 392 256 5 253 639 101 489 1.77e-63 218
MS.gene052096.t1 AT5G48150 32.653 392 256 5 253 639 101 489 1.77e-63 218
MS.gene052096.t1 AT1G50600 33.422 374 242 4 270 639 155 525 2.77e-63 218
MS.gene052096.t1 AT1G50600 33.422 374 242 4 270 639 230 600 5.26e-63 219
MS.gene052096.t1 AT1G50600 33.422 374 242 4 270 639 226 596 5.39e-63 219
MS.gene052096.t1 AT2G04890 31.351 370 248 3 270 639 49 412 2.50e-62 213
MS.gene052096.t1 AT1G21450 28.253 538 332 14 130 639 81 592 9.16e-55 197
MS.gene052096.t1 AT4G17230 30.239 377 256 4 269 641 153 526 8.76e-52 187
MS.gene052096.t1 AT4G17230 30.239 377 256 4 269 641 153 526 8.76e-52 187
MS.gene052096.t1 AT3G54220 33.081 396 248 9 250 641 269 651 1.18e-51 189
MS.gene052096.t1 AT1G66350 31.316 380 238 8 264 639 145 505 5.62e-49 179
MS.gene052096.t1 AT3G03450 29.534 386 248 9 264 639 173 544 1.21e-48 179
MS.gene052096.t1 AT2G01570 29.948 384 244 9 264 639 214 580 8.78e-46 171
MS.gene052096.t1 AT5G17490 27.778 378 255 7 266 639 152 515 1.15e-44 167
MS.gene052096.t1 AT1G14920 28.534 382 252 7 264 639 162 528 3.17e-44 166
MS.gene052096.t1 AT5G66770 30.541 370 239 9 278 639 224 583 2.28e-38 150
MS.gene052096.t1 AT1G50420 23.490 447 278 8 253 639 36 478 4.36e-37 145
MS.gene052096.t1 AT1G63100 28.212 358 240 8 262 614 269 614 6.77e-34 137
MS.gene052096.t1 AT1G63100 28.212 358 240 8 262 614 244 589 7.10e-34 137
MS.gene052096.t1 AT4G37650 26.257 358 234 9 273 607 145 495 4.89e-33 134
MS.gene052096.t1 AT3G50650 27.147 361 234 9 294 639 195 541 3.17e-32 131
MS.gene052096.t1 AT5G41920 29.211 380 244 12 268 639 37 399 5.32e-32 129
MS.gene052096.t1 AT5G52510 25.065 387 258 9 272 639 266 639 2.17e-29 124
MS.gene052096.t1 AT1G55580 27.163 416 244 11 271 639 41 444 1.09e-28 120
MS.gene052096.t1 AT3G49950 24.490 392 266 9 271 641 27 409 2.98e-25 108
MS.gene052096.t1 AT4G08250 24.936 389 268 12 266 639 97 476 8.73e-23 102
MS.gene052096.t1 AT3G13840 24.021 383 253 13 274 639 148 509 4.18e-12 69.3

Find 113 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTCTCTCCAACAAATT+TGG 0.135021 2.3:+5609403 None:intergenic
AGCCTTCACATTATAGATTT+TGG 0.143420 2.3:-5609520 MS.gene052096:CDS
GGAGGAATTGGAAGAGTATT+TGG 0.153984 2.3:+5610147 None:intergenic
AATCTTCTTCCATGAGAAAT+TGG 0.203759 2.3:+5610478 None:intergenic
CTTGGTACTAAAGTAATAAA+AGG 0.213486 2.3:-5609958 MS.gene052096:CDS
CAAAGGTTGGCACATTTCTT+TGG 0.213920 2.3:-5609727 MS.gene052096:CDS
GAATTCTCTTCAACTGTTCT+TGG 0.222104 2.3:-5610606 MS.gene052096:CDS
TGGGTTTAAGTCTTTGAAAT+TGG 0.243482 2.3:-5608891 MS.gene052096:CDS
TATCCCTGTGATCCTCAATT+TGG 0.247519 2.3:+5609432 None:intergenic
GAATCCAAGGGGTGCTGTTT+TGG 0.248365 2.3:-5609197 MS.gene052096:CDS
TCTTAATCTGGTTTAATAAC+TGG 0.258792 2.3:+5609779 None:intergenic
GAAACAAAGTTTGGAGGAAT+TGG 0.270095 2.3:+5610135 None:intergenic
TTCTATTTGGTTCTTCTTGT+TGG 0.274191 2.3:-5608757 MS.gene052096:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2.3:+5609810 None:intergenic
TGTTTGAGGTGAATGAAAAT+TGG 0.282920 2.3:-5608805 MS.gene052096:CDS
ATTCACTCTCCCTTTGTTCA+TGG 0.291853 2.3:+5610625 None:intergenic
TAGTTGTATGAGTCTATAAT+TGG 0.294656 2.3:+5610192 None:intergenic
CAAACAGATTCTATGGTTAT+AGG 0.296898 2.3:-5609894 MS.gene052096:CDS
AGTAATTTATCAAATAATTC+TGG 0.301090 2.3:+5609985 None:intergenic
TTTGTTCATAGCATTGTTAA+TGG 0.316703 2.3:-5609145 MS.gene052096:CDS
GAGAAGAATGTGTCTTAATC+TGG 0.320149 2.3:+5609767 None:intergenic
GAGGAATTGGAAGAGTATTT+GGG 0.326156 2.3:+5610148 None:intergenic
TGGAAGGGTAGAGTTCTATT+TGG 0.329745 2.3:-5608770 MS.gene052096:CDS
TCGAATTGAGAAACAAAGTT+TGG 0.339338 2.3:+5610126 None:intergenic
GCAACTTATTGCGAACGTTT+CGG 0.343573 2.3:-5609361 MS.gene052096:CDS
GAATTATCAAACAGATTCTA+TGG 0.344805 2.3:-5609901 MS.gene052096:CDS
TGATGTTTGAGGAAGAGTTT+TGG 0.352833 2.3:-5609009 MS.gene052096:CDS
TGGAAGACTCTTCATGTTCA+TGG 0.366829 2.3:+5610520 None:intergenic
AGAGTATTTGGGAGTGGAAA+AGG 0.382981 2.3:+5610159 None:intergenic
ATCCCTGTGATCCTCAATTT+GGG 0.383992 2.3:+5609433 None:intergenic
GAAGAAATCTGCTGCAGATA+TGG 0.397365 2.3:-5609641 MS.gene052096:CDS
GAGTGAATGGAACAGGTCTT+AGG 0.405752 2.3:-5609387 MS.gene052096:CDS
ATGCTATGAACAAATATATT+CGG 0.406151 2.3:+5609154 None:intergenic
GTTGAAGAGCAAAGGAATCT+TGG 0.406232 2.3:+5610433 None:intergenic
GCAATACCATTGAACTCAAA+AGG 0.418002 2.3:+5609334 None:intergenic
GATAGATCTTCCAAATTTGT+TGG 0.422261 2.3:-5609413 MS.gene052096:CDS
AAACTATAATCACTGATGTC+AGG 0.427646 2.3:+5610249 None:intergenic
TTGAGAGGCCAGAGACATAT+AGG 0.428001 2.3:-5608940 MS.gene052096:CDS
TTTGATAAATTACTAGTCCT+TGG 0.440112 2.3:-5609976 MS.gene052096:CDS
ATGAGGAAGTTGTGGATTTG+AGG 0.441434 2.3:-5609858 MS.gene052096:CDS
TAGCTTGTGAAGGCTGTGAT+AGG 0.447026 2.3:-5608964 MS.gene052096:CDS
ACTCTTCATGTTCATGGTGT+TGG 0.448056 2.3:+5610526 None:intergenic
TTGGAGAGAGTGAATGGAAC+AGG 0.449006 2.3:-5609394 MS.gene052096:CDS
AAGACACATTCTTCTCCAAC+AGG 0.450645 2.3:-5609760 MS.gene052096:CDS
AAAGCACGTTTAGCAGGAAC+TGG 0.455780 2.3:-5609694 MS.gene052096:CDS
CTTTGAGTTCCATCACCTGT+TGG 0.463702 2.3:+5609745 None:intergenic
TGCCATTGCCTATATGTCTC+TGG 0.465075 2.3:+5608932 None:intergenic
CACTTGAAAACACAAGCTTT+CGG 0.465170 2.3:-5609933 MS.gene052096:CDS
AATTTGTTGGAGAGAGTGAA+TGG 0.471019 2.3:-5609400 MS.gene052096:CDS
TCCTAGTAGGTTGATGTTTG+AGG 0.474185 2.3:-5609020 MS.gene052096:CDS
GAAGAGTCTTCCAATGCTCT+TGG 0.479029 2.3:-5610510 MS.gene052096:CDS
GAGTATTTGGGAGTGGAAAA+GGG 0.483840 2.3:+5610160 None:intergenic
CATCGTTTATCGAAAAGATC+TGG 0.488523 2.3:-5609463 MS.gene052096:CDS
GCACTTAAAGCACGTTTAGC+AGG 0.497655 2.3:-5609700 MS.gene052096:CDS
CATGTTGTGAAGCTCTATGC+TGG 0.501576 2.3:+5608731 None:intergenic
TCCTCAAACATCAACCTACT+AGG 0.501588 2.3:+5609019 None:intergenic
ACTTGATGAGACAGTTGCTT+CGG 0.501704 2.3:-5609221 MS.gene052096:CDS
CCACCCAAATTGAGGATCAC+AGG 0.502169 2.3:-5609436 MS.gene052096:CDS
GTTAATATAACCAAGAGCAT+TGG 0.505462 2.3:+5610500 None:intergenic
CTCAGTAAGTTGAAGAGCAA+AGG 0.507074 2.3:+5610425 None:intergenic
TAGGAGACAAAGAAGTTATG+AGG 0.508963 2.3:-5609875 MS.gene052096:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2.3:-5608790 MS.gene052096:CDS
GCAGTAGCAGTAGGAGGAGG+AGG 0.511002 2.3:+5610360 None:intergenic
GAAGTGAAAGAAGGAGGAGG+AGG 0.513604 2.3:+5610330 None:intergenic
CAGTTGCTTCGGAGAATCCA+AGG 0.513959 2.3:-5609210 MS.gene052096:CDS
CATTCTTCTCCAACAGGTGA+TGG 0.515669 2.3:-5609754 MS.gene052096:CDS
ATTGGAAGAGTATTTGGGAG+TGG 0.520042 2.3:+5610153 None:intergenic
TTGGTACTAAAGTAATAAAA+GGG 0.520896 2.3:-5609957 MS.gene052096:CDS
AATTGAGAAACAAAGTTTGG+AGG 0.522706 2.3:+5610129 None:intergenic
TACCTCATGAGAAGGGAAAA+CGG 0.524121 2.3:+5610098 None:intergenic
TTATATTAACCAATTTCTCA+TGG 0.526067 2.3:-5610487 MS.gene052096:CDS
AAGAAGTTATGAGGAAGTTG+TGG 0.533441 2.3:-5609866 MS.gene052096:CDS
GCATGTTATAGATAAATTGA+AGG 0.536806 2.3:-5608864 MS.gene052096:CDS
CATGTTATAGATAAATTGAA+GGG 0.543479 2.3:-5608863 MS.gene052096:CDS
GTCTTAATTACCTCATGAGA+AGG 0.545201 2.3:+5610090 None:intergenic
AAATCCAAAACAGCACCCCT+TGG 0.545355 2.3:+5609193 None:intergenic
GGCATGCTGATGAATAAACT+TGG 0.547453 2.3:+5609608 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2.3:-5608786 MS.gene052096:CDS
TTGGGAAAGTATCAAAGTTG+AGG 0.550259 2.3:-5609305 MS.gene052096:CDS
GTAGCAGTAGGAGGAGGAGG+AGG 0.552690 2.3:+5610363 None:intergenic
GTTCATAGCATTGTTAATGG+TGG 0.554304 2.3:-5609142 MS.gene052096:CDS
AACTATAATCACTGATGTCA+GGG 0.555622 2.3:+5610250 None:intergenic
TGATTCACACCATGAACAAA+GGG 0.556404 2.3:-5610634 MS.gene052096:CDS
GCAGCAGTAGCAGTAGGAGG+AGG 0.561458 2.3:+5610357 None:intergenic
GGAGCAGCAGCAGTAGCAGT+AGG 0.562763 2.3:+5610351 None:intergenic
GACGACGAAGAAGTGAAAGA+AGG 0.569183 2.3:+5610321 None:intergenic
TATAATTGGATCAAGTTCAA+AGG 0.570239 2.3:+5610206 None:intergenic
CACCAAAATCTATAATGTGA+AGG 0.575769 2.3:+5609518 None:intergenic
TTGAACGTGAGGATCCTAGT+AGG 0.577844 2.3:-5609033 MS.gene052096:CDS
GAAGAAGTGAAAGAAGGAGG+AGG 0.579367 2.3:+5610327 None:intergenic
GGCCAGAGACATATAGGCAA+TGG 0.582352 2.3:-5608934 MS.gene052096:CDS
GAATGGAACAGGTCTTAGGC+TGG 0.584610 2.3:-5609383 MS.gene052096:CDS
CATGGAAGATGATGACATAG+AGG 0.588846 2.3:-5610565 MS.gene052096:CDS
CGTTTATCGAAAAGATCTGG+TGG 0.590533 2.3:-5609460 MS.gene052096:CDS
TCTTAATTACCTCATGAGAA+GGG 0.597606 2.3:+5610091 None:intergenic
TTGATTCACACCATGAACAA+AGG 0.599099 2.3:-5610635 MS.gene052096:CDS
CACCCAAATTGAGGATCACA+GGG 0.601284 2.3:-5609435 MS.gene052096:CDS
AGTTGCTTCGGAGAATCCAA+GGG 0.606761 2.3:-5609209 MS.gene052096:CDS
CTGGTGGGCCACCCAAATTG+AGG 0.614366 2.3:-5609444 MS.gene052096:CDS
GCAGCAGCAGTAGCAGTAGG+AGG 0.617587 2.3:+5610354 None:intergenic
CCTGTGATCCTCAATTTGGG+TGG 0.623258 2.3:+5609436 None:intergenic
TTGTTGTAGATGGACAAAAG+AGG 0.629088 2.3:-5610660 None:intergenic
AGGTGATGGAACTCAAAGGT+TGG 0.629359 2.3:-5609740 MS.gene052096:CDS
GACGAAGAAGTGAAAGAAGG+AGG 0.633102 2.3:+5610324 None:intergenic
CAACAGGTGATGGAACTCAA+AGG 0.635236 2.3:-5609744 MS.gene052096:CDS
AATGAAAATTGGATGCTACA+AGG 0.641663 2.3:-5608794 MS.gene052096:CDS
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2.3:-5608785 MS.gene052096:CDS
TAACAACAATGTTGAACGTG+AGG 0.650267 2.3:-5609044 MS.gene052096:CDS
GATAAACGATGTATAAAACC+AGG 0.661852 2.3:+5609475 None:intergenic
ATGAATGTTATAGCTTGTGA+AGG 0.662961 2.3:-5608974 MS.gene052096:CDS
GTTTATCGAAAAGATCTGGT+GGG 0.689733 2.3:-5609459 MS.gene052096:CDS
AAGGCTGTGATAGGATTGAG+AGG 0.704778 2.3:-5608955 MS.gene052096:CDS
GTTGCTTCGGAGAATCCAAG+GGG 0.756075 2.3:-5609208 MS.gene052096:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAATTTATCAAATAATTC+TGG + chr2.3:5609414-5609433 None:intergenic 15.0%
!!! TTTTATAAACAAATTTCTCA+TGG - chr2.3:5608813-5608832 MS.gene052096:CDS 15.0%
!! ATGCTATGAACAAATATATT+CGG + chr2.3:5610245-5610264 None:intergenic 20.0%
!! CATGTTATAGATAAATTGAA+GGG - chr2.3:5610533-5610552 MS.gene052096:CDS 20.0%
!! TGAAATATCATTACAAGAAA+TGG + chr2.3:5609589-5609608 None:intergenic 20.0%
!! TTATATTAACCAATTTCTCA+TGG - chr2.3:5608909-5608928 MS.gene052096:CDS 20.0%
!! TTGGTACTAAAGTAATAAAA+GGG - chr2.3:5609439-5609458 MS.gene052096:CDS 20.0%
! CTTGGTACTAAAGTAATAAA+AGG - chr2.3:5609438-5609457 MS.gene052096:CDS 25.0%
! GAATTATCAAACAGATTCTA+TGG - chr2.3:5609495-5609514 MS.gene052096:CDS 25.0%
! GCATGTTATAGATAAATTGA+AGG - chr2.3:5610532-5610551 MS.gene052096:CDS 25.0%
! TAGTTGTATGAGTCTATAAT+TGG + chr2.3:5609207-5609226 None:intergenic 25.0%
! TATAATTGGATCAAGTTCAA+AGG + chr2.3:5609193-5609212 None:intergenic 25.0%
! TTAAACCTTGTCACAAAAAA+AGG + chr2.3:5610290-5610309 None:intergenic 25.0%
! TTTGATAAATTACTAGTCCT+TGG - chr2.3:5609420-5609439 MS.gene052096:CDS 25.0%
! TTTGTTCATAGCATTGTTAA+TGG - chr2.3:5610251-5610270 MS.gene052096:CDS 25.0%
!! AAGTTGAGGATTTTATGATA+AGG - chr2.3:5610105-5610124 MS.gene052096:CDS 25.0%
!! ATAATTGCAAGCTTTTCAAA+AGG + chr2.3:5609813-5609832 None:intergenic 25.0%
!! CTTTTATTACTTTAGTACCA+AGG + chr2.3:5609440-5609459 None:intergenic 25.0%
!! TAATAGTGATTTTCTGTTTG+AGG - chr2.3:5610577-5610596 MS.gene052096:CDS 25.0%
!! TAATTGCAAGCTTTTCAAAA+GGG + chr2.3:5609812-5609831 None:intergenic 25.0%
!! TCTTAATCTGGTTTAATAAC+TGG + chr2.3:5609620-5609639 None:intergenic 25.0%
!! TTTGATACTTTCCCAATTTT+TGG + chr2.3:5610087-5610106 None:intergenic 25.0%
!!! TGTTTTGGATTTGATTAAGA+AGG - chr2.3:5610214-5610233 MS.gene052096:CDS 25.0%
!!! TTTTTTTGTGACAAGGTTTA+AGG - chr2.3:5610289-5610308 MS.gene052096:CDS 25.0%
AAACTATAATCACTGATGTC+AGG + chr2.3:5609150-5609169 None:intergenic 30.0%
AACTATAATCACTGATGTCA+GGG + chr2.3:5609149-5609168 None:intergenic 30.0%
AATCTTCTTCCATGAGAAAT+TGG + chr2.3:5608921-5608940 None:intergenic 30.0%
AATGAAAATTGGATGCTACA+AGG - chr2.3:5610602-5610621 MS.gene052096:CDS 30.0%
AATTGAGAAACAAAGTTTGG+AGG + chr2.3:5609270-5609289 None:intergenic 30.0%
CAAACAGATTCTATGGTTAT+AGG - chr2.3:5609502-5609521 MS.gene052096:CDS 30.0%
CAATGGTATTGCCAAAAATT+GGG - chr2.3:5610073-5610092 MS.gene052096:CDS 30.0%
CACCAAAATCTATAATGTGA+AGG + chr2.3:5609881-5609900 None:intergenic 30.0%
GATAAACGATGTATAAAACC+AGG + chr2.3:5609924-5609943 None:intergenic 30.0%
GATAGATCTTCCAAATTTGT+TGG - chr2.3:5609983-5610002 MS.gene052096:CDS 30.0%
TCAATGGTATTGCCAAAAAT+TGG - chr2.3:5610072-5610091 MS.gene052096:CDS 30.0%
TCGAATTGAGAAACAAAGTT+TGG + chr2.3:5609273-5609292 None:intergenic 30.0%
TCTTAATTACCTCATGAGAA+GGG + chr2.3:5609308-5609327 None:intergenic 30.0%
! AGCCTTCACATTATAGATTT+TGG - chr2.3:5609876-5609895 MS.gene052096:CDS 30.0%
! ATGAATGTTATAGCTTGTGA+AGG - chr2.3:5610422-5610441 MS.gene052096:CDS 30.0%
! GTTAATATAACCAAGAGCAT+TGG + chr2.3:5608899-5608918 None:intergenic 30.0%
! TGTTTGAGGTGAATGAAAAT+TGG - chr2.3:5610591-5610610 MS.gene052096:CDS 30.0%
!! CACATTATAGATTTTGGTGT+TGG - chr2.3:5609882-5609901 MS.gene052096:CDS 30.0%
!! TGGGTTTAAGTCTTTGAAAT+TGG - chr2.3:5610505-5610524 MS.gene052096:CDS 30.0%
!! TTCTATTTGGTTCTTCTTGT+TGG - chr2.3:5610639-5610658 MS.gene052096:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG - chr2.3:5609888-5609907 MS.gene052096:CDS 30.0%
!!! ATTTGTAGTTTTGTTGATGC+TGG + chr2.3:5609116-5609135 None:intergenic 30.0%
!!! GGAAGCTGTTTTTCATTATT+CGG - chr2.3:5610310-5610329 MS.gene052096:CDS 30.0%
AAAATTGGATGCTACAAGGT+TGG - chr2.3:5610606-5610625 MS.gene052096:CDS 35.0%
AAGAAGTTATGAGGAAGTTG+TGG - chr2.3:5609530-5609549 MS.gene052096:CDS 35.0%
CACTTGAAAACACAAGCTTT+CGG - chr2.3:5609463-5609482 MS.gene052096:CDS 35.0%
CATCGTTTATCGAAAAGATC+TGG - chr2.3:5609933-5609952 MS.gene052096:CDS 35.0%
GAAACAAAGTTTGGAGGAAT+TGG + chr2.3:5609264-5609283 None:intergenic 35.0%
GAATTCTCTTCAACTGTTCT+TGG - chr2.3:5608790-5608809 MS.gene052096:CDS 35.0%
GAGAAGAATGTGTCTTAATC+TGG + chr2.3:5609632-5609651 None:intergenic 35.0%
GCAATACCATTGAACTCAAA+AGG + chr2.3:5610065-5610084 None:intergenic 35.0%
GTCTTAATTACCTCATGAGA+AGG + chr2.3:5609309-5609328 None:intergenic 35.0%
GTTCATAGCATTGTTAATGG+TGG - chr2.3:5610254-5610273 MS.gene052096:CDS 35.0%
GTTTATCGAAAAGATCTGGT+GGG - chr2.3:5609937-5609956 MS.gene052096:CDS 35.0%
TAACAACAATGTTGAACGTG+AGG - chr2.3:5610352-5610371 MS.gene052096:CDS 35.0%
TAGGAGACAAAGAAGTTATG+AGG - chr2.3:5609521-5609540 MS.gene052096:CDS 35.0%
TGATTCACACCATGAACAAA+GGG - chr2.3:5608762-5608781 MS.gene052096:CDS 35.0%
TTCACTCTCTCCAACAAATT+TGG + chr2.3:5609996-5610015 None:intergenic 35.0%
TTGATTCACACCATGAACAA+AGG - chr2.3:5608761-5608780 MS.gene052096:CDS 35.0%
! ATGTTTGAGGAAGAGTTTTG+GGG - chr2.3:5610389-5610408 MS.gene052096:CDS 35.0%
! ATTTTAGGCACATGGGAAAT+GGG - chr2.3:5610486-5610505 MS.gene052096:CDS 35.0%
! CATATAGGCAATGGCATTTT+AGG - chr2.3:5610471-5610490 MS.gene052096:CDS 35.0%
! GATGTTTGAGGAAGAGTTTT+GGG - chr2.3:5610388-5610407 MS.gene052096:CDS 35.0%
! TGATGTTTGAGGAAGAGTTT+TGG - chr2.3:5610387-5610406 MS.gene052096:CDS 35.0%
!! AATTTGTTGGAGAGAGTGAA+TGG - chr2.3:5609996-5610015 MS.gene052096:CDS 35.0%
!! GAGGAATTGGAAGAGTATTT+GGG + chr2.3:5609251-5609270 None:intergenic 35.0%
!! TTGGGAAAGTATCAAAGTTG+AGG - chr2.3:5610091-5610110 MS.gene052096:CDS 35.0%
!!! ATGCACCTTTTTTTGTGACA+AGG - chr2.3:5610282-5610301 MS.gene052096:CDS 35.0%
!!! GTGTTGGTTATGGTTTTAAG+TGG - chr2.3:5609898-5609917 MS.gene052096:CDS 35.0%
AAGACACATTCTTCTCCAAC+AGG - chr2.3:5609636-5609655 MS.gene052096:CDS 40.0%
ACTCTTCATGTTCATGGTGT+TGG + chr2.3:5608873-5608892 None:intergenic 40.0%
ACTTGATGAGACAGTTGCTT+CGG - chr2.3:5610175-5610194 MS.gene052096:CDS 40.0%
ATCCCTGTGATCCTCAATTT+GGG + chr2.3:5609966-5609985 None:intergenic 40.0%
ATGAGGAAGTTGTGGATTTG+AGG - chr2.3:5609538-5609557 MS.gene052096:CDS 40.0%
ATTCACTCTCCCTTTGTTCA+TGG + chr2.3:5608774-5608793 None:intergenic 40.0%
CAAAGGTTGGCACATTTCTT+TGG - chr2.3:5609669-5609688 MS.gene052096:CDS 40.0%
CATGGAAGATGATGACATAG+AGG - chr2.3:5608831-5608850 MS.gene052096:CDS 40.0%
CGTTTATCGAAAAGATCTGG+TGG - chr2.3:5609936-5609955 MS.gene052096:CDS 40.0%
GAAGAAATCTGCTGCAGATA+TGG - chr2.3:5609755-5609774 MS.gene052096:CDS 40.0%
GAGTATTTGGGAGTGGAAAA+GGG + chr2.3:5609239-5609258 None:intergenic 40.0%
GCAACTTATTGCGAACGTTT+CGG - chr2.3:5610035-5610054 MS.gene052096:CDS 40.0%
GGCATGCTGATGAATAAACT+TGG + chr2.3:5609791-5609810 None:intergenic 40.0%
GTTGAAGAGCAAAGGAATCT+TGG + chr2.3:5608966-5608985 None:intergenic 40.0%
TACCTCATGAGAAGGGAAAA+CGG + chr2.3:5609301-5609320 None:intergenic 40.0%
TATCCCTGTGATCCTCAATT+TGG + chr2.3:5609967-5609986 None:intergenic 40.0%
TCCTCAAACATCAACCTACT+AGG + chr2.3:5610380-5610399 None:intergenic 40.0%
TGGAAGACTCTTCATGTTCA+TGG + chr2.3:5608879-5608898 None:intergenic 40.0%
TGGAAGGGTAGAGTTCTATT+TGG - chr2.3:5610626-5610645 MS.gene052096:CDS 40.0%
! AGAGTATTTGGGAGTGGAAA+AGG + chr2.3:5609240-5609259 None:intergenic 40.0%
! CAATGGCATTTTAGGCACAT+GGG - chr2.3:5610479-5610498 MS.gene052096:CDS 40.0%
! CATTTTAGGCACATGGGAAA+TGG - chr2.3:5610485-5610504 MS.gene052096:CDS 40.0%
! CTCAGTAAGTTGAAGAGCAA+AGG + chr2.3:5608974-5608993 None:intergenic 40.0%
!! ATTGGAAGAGTATTTGGGAG+TGG + chr2.3:5609246-5609265 None:intergenic 40.0%
!! CGAAAGCTTGTGTTTTCAAG+TGG + chr2.3:5609465-5609484 None:intergenic 40.0%
!! GGAGGAATTGGAAGAGTATT+TGG + chr2.3:5609252-5609271 None:intergenic 40.0%
!! TCCTAGTAGGTTGATGTTTG+AGG - chr2.3:5610376-5610395 MS.gene052096:CDS 40.0%
!! TGTTTGAGGAAGAGTTTTGG+GGG - chr2.3:5610390-5610409 MS.gene052096:CDS 40.0%
!!! GGTGTTCCTTTTGAGTTCAA+TGG - chr2.3:5610056-5610075 MS.gene052096:CDS 40.0%
!!! GTAGTTTTGTTGATGCTGGA+TGG + chr2.3:5609112-5609131 None:intergenic 40.0%
AAAGCACGTTTAGCAGGAAC+TGG - chr2.3:5609702-5609721 MS.gene052096:CDS 45.0%
AAATCCAAAACAGCACCCCT+TGG + chr2.3:5610206-5610225 None:intergenic 45.0%
AAGGCTGTGATAGGATTGAG+AGG - chr2.3:5610441-5610460 MS.gene052096:CDS 45.0%
AGTTGCTTCGGAGAATCCAA+GGG - chr2.3:5610187-5610206 MS.gene052096:CDS 45.0%
CACCCAAATTGAGGATCACA+GGG - chr2.3:5609961-5609980 MS.gene052096:CDS 45.0%
CATTCTTCTCCAACAGGTGA+TGG - chr2.3:5609642-5609661 MS.gene052096:CDS 45.0%
GAAGAAGTGAAAGAAGGAGG+AGG + chr2.3:5609072-5609091 None:intergenic 45.0%
GAAGAGTCTTCCAATGCTCT+TGG - chr2.3:5608886-5608905 MS.gene052096:CDS 45.0%
GACGAAGAAGTGAAAGAAGG+AGG + chr2.3:5609075-5609094 None:intergenic 45.0%
GACGACGAAGAAGTGAAAGA+AGG + chr2.3:5609078-5609097 None:intergenic 45.0%
GAGTGAATGGAACAGGTCTT+AGG - chr2.3:5610009-5610028 MS.gene052096:CDS 45.0%
GCACTTAAAGCACGTTTAGC+AGG - chr2.3:5609696-5609715 MS.gene052096:CDS 45.0%
TGCCATTGCCTATATGTCTC+TGG + chr2.3:5610467-5610486 None:intergenic 45.0%
TGGATGCTACAAGGTTGGAA+GGG - chr2.3:5610611-5610630 MS.gene052096:CDS 45.0%
TTGAACGTGAGGATCCTAGT+AGG - chr2.3:5610363-5610382 MS.gene052096:CDS 45.0%
TTGAGAGGCCAGAGACATAT+AGG - chr2.3:5610456-5610475 MS.gene052096:CDS 45.0%
TTGGAGAGAGTGAATGGAAC+AGG - chr2.3:5610002-5610021 MS.gene052096:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG - chr2.3:5610610-5610629 MS.gene052096:CDS 45.0%
! AACCGTTTTCCCTTCTCATG+AGG - chr2.3:5609296-5609315 MS.gene052096:CDS 45.0%
! CTTTGAGTTCCATCACCTGT+TGG + chr2.3:5609654-5609673 None:intergenic 45.0%
! GCAATGGCATTTTAGGCACA+TGG - chr2.3:5610478-5610497 MS.gene052096:CDS 45.0%
! TAGCTTGTGAAGGCTGTGAT+AGG - chr2.3:5610432-5610451 MS.gene052096:CDS 45.0%
!! AGGTGATGGAACTCAAAGGT+TGG - chr2.3:5609656-5609675 MS.gene052096:CDS 45.0%
!! CAACAGGTGATGGAACTCAA+AGG - chr2.3:5609652-5609671 MS.gene052096:CDS 45.0%
!!! GGTTATGGTTTTAAGTGGCC+TGG - chr2.3:5609903-5609922 MS.gene052096:CDS 45.0%
CAGTTGCTTCGGAGAATCCA+AGG - chr2.3:5610186-5610205 MS.gene052096:CDS 50.0%
CCACCCAAATTGAGGATCAC+AGG - chr2.3:5609960-5609979 MS.gene052096:CDS 50.0%
CCTGTGATCCTCAATTTGGG+TGG + chr2.3:5609963-5609982 None:intergenic 50.0%
GAAGTGAAAGAAGGAGGAGG+AGG + chr2.3:5609069-5609088 None:intergenic 50.0%
GAATGGAACAGGTCTTAGGC+TGG - chr2.3:5610013-5610032 MS.gene052096:CDS 50.0%
GGCCAGAGACATATAGGCAA+TGG - chr2.3:5610462-5610481 MS.gene052096:CDS 50.0%
GTTGCTTCGGAGAATCCAAG+GGG - chr2.3:5610188-5610207 MS.gene052096:CDS 50.0%
!!! GAATCCAAGGGGTGCTGTTT+TGG - chr2.3:5610199-5610218 MS.gene052096:CDS 50.0%
!!! TGAGGAAGAGTTTTGGGGGA+AGG - chr2.3:5610394-5610413 MS.gene052096:CDS 50.0%
GCAGCAGCAGTAGCAGTAGG+AGG + chr2.3:5609045-5609064 None:intergenic 60.0%
GCAGCAGTAGCAGTAGGAGG+AGG + chr2.3:5609042-5609061 None:intergenic 60.0%
GCAGTAGCAGTAGGAGGAGG+AGG + chr2.3:5609039-5609058 None:intergenic 60.0%
GGAGCAGCAGCAGTAGCAGT+AGG + chr2.3:5609048-5609067 None:intergenic 60.0%
GTAGCAGTAGGAGGAGGAGG+AGG + chr2.3:5609036-5609055 None:intergenic 60.0%
! CTGGTGGGCCACCCAAATTG+AGG - chr2.3:5609952-5609971 MS.gene052096:CDS 60.0%
GCAGTAGGAGGAGGAGGAGG+AGG + chr2.3:5609033-5609052 None:intergenic 65.0%
GTAGGAGGAGGAGGAGGAGG+AGG + chr2.3:5609030-5609049 None:intergenic 65.0%
GGAGGAGGAGGAGGAGGAGG+AGG + chr2.3:5609024-5609043 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5608745 5610673 5608745 ID=MS.gene052096
chr2.3 mRNA 5608745 5610673 5608745 ID=MS.gene052096.t1;Parent=MS.gene052096
chr2.3 exon 5608745 5610673 5608745 ID=MS.gene052096.t1.exon1;Parent=MS.gene052096.t1
chr2.3 CDS 5608745 5610673 5608745 ID=cds.MS.gene052096.t1;Parent=MS.gene052096.t1
Gene Sequence

>MS.gene052096

ATGGACAAAAGAGGTGTTGATTCACACCATGAACAAAGGGAGAGTGAATTCTCTTCAACTGTTCTTGGTTTTATAAACAAATTTCTCATGGAAGATGATGACATAGAGGAAGACTACAATATGTTCCAACACCATGAACATGAAGAGTCTTCCAATGCTCTTGGTTATATTAACCAATTTCTCATGGAAGAAGATTTTGAAAAAGAATACACTATGTTCCAAGATTCCTTTGCTCTTCAACTTACTGAGAAATCATTTCATGATGTTATTTCTCACACTCCTCCTCCTCCTCCTCCTCCTCCTACTGCTACTGCTGCTGCTCCTCCTCCTCCTTCTTTCACTTCTTCGTCGTCTTCTTCTTCTTCCATCCAGCATCAACAAAACTACAAATTTGTGCATAGCCCTGACATCAGTGATTATAGTTTCAGCAGTGTTAGCACTAGCTCCTTTGAACTTGATCCAATTATAGACTCATACAACTACAATTACAACCCTTTTCCACTCCCAAATACTCTTCCAATTCCTCCAAACTTTGTTTCTCAATTCGACTCAACCGTTTTCCCTTCTCATGAGGTAATTAAGACAGAAAACTTTGAAGAAGAGCATTTTCTCAATGTGTCACAAAATGAGTCAGAACAAGTATATGTAGATGATAACAGTGAGTTACCAGAATTATTTGATAAATTACTAGTCCTTGGTACTAAAGTAATAAAAGGGCCACTTGAAAACACAAGCTTTCGGCAGAATGAAGAATTATCAAACAGATTCTATGGTTATAGGAGACAAAGAAGTTATGAGGAAGTTGTGGATTTGAGGACACTATTGATGCTATGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAGTTATTAAACCAGATTAAGACACATTCTTCTCCAACAGGTGATGGAACTCAAAGGTTGGCACATTTCTTTGGAAATGCACTTAAAGCACGTTTAGCAGGAACTGGTTCACAAATCTATAGAGCTCTATCTTCGAAGAAGAAATCTGCTGCAGATATGGCAAGAGCTTACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAGCTTGCAATTATGTTCTCAAACAATGCAATTTTTAATGTAGCAAAAGAAACAGAAAGCCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTTAAGTGGCCTGGTTTTATACATCGTTTATCGAAAAGATCTGGTGGGCCACCCAAATTGAGGATCACAGGGATAGATCTTCCAAATTTGTTGGAGAGAGTGAATGGAACAGGTCTTAGGCTGGCAACTTATTGCGAACGTTTCGGTGTTCCTTTTGAGTTCAATGGTATTGCCAAAAATTGGGAAAGTATCAAAGTTGAGGATTTTATGATAAGGAAAAATGAGTTTGTTGCTGTGAATTGTTATTTTAAATTTGAAAATCTACTTGATGAGACAGTTGCTTCGGAGAATCCAAGGGGTGCTGTTTTGGATTTGATTAAGAAGGCAAATCCGAATATATTTGTTCATAGCATTGTTAATGGTGGTTATGATGCACCTTTTTTTGTGACAAGGTTTAAGGAAGCTGTTTTTCATTATTCGGCATTGTTTGATATGCTTGATAACAACAATGTTGAACGTGAGGATCCTAGTAGGTTGATGTTTGAGGAAGAGTTTTGGGGGAAGGATATAATGAATGTTATAGCTTGTGAAGGCTGTGATAGGATTGAGAGGCCAGAGACATATAGGCAATGGCATTTTAGGCACATGGGAAATGGGTTTAAGTCTTTGAAATTGGATAAGCATGTTATAGATAAATTGAAGGGTAAGTTGAGAGATGATGCATATAATAGTGATTTTCTGTTTGAGGTGAATGAAAATTGGATGCTACAAGGTTGGAAGGGTAGAGTTCTATTTGGTTCTTCTTGTTGGATTCCAGCATAG
Protein sequence

>MS.gene052096.t1

MDKRGVDSHHEQRESEFSSTVLGFINKFLMEDDDIEEDYNMFQHHEHEESSNALGYINQFLMEEDFEKEYTMFQDSFALQLTEKSFHDVISHTPPPPPPPPTATAAAPPPPSFTSSSSSSSSIQHQQNYKFVHSPDISDYSFSSVSTSSFELDPIIDSYNYNYNPFPLPNTLPIPPNFVSQFDSTVFPSHEVIKTENFEEEHFLNVSQNESEQVYVDDNSELPELFDKLLVLGTKVIKGPLENTSFRQNEELSNRFYGYRRQRSYEEVVDLRTLLMLCAQSISCNDISNANQLLNQIKTHSSPTGDGTQRLAHFFGNALKARLAGTGSQIYRALSSKKKSAADMARAYQVYSSACPFEKLAIMFSNNAIFNVAKETESLHIIDFGVGYGFKWPGFIHRLSKRSGGPPKLRITGIDLPNLLERVNGTGLRLATYCERFGVPFEFNGIAKNWESIKVEDFMIRKNEFVAVNCYFKFENLLDETVASENPRGAVLDLIKKANPNIFVHSIVNGGYDAPFFVTRFKEAVFHYSALFDMLDNNNVEREDPSRLMFEEEFWGKDIMNVIACEGCDRIERPETYRQWHFRHMGNGFKSLKLDKHVIDKLKGKLRDDAYNSDFLFEVNENWMLQGWKGRVLFGSSCWIPA