AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052097

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052097.t1 MTR_2g097310 87.786 655 62 3 1 652 1 640 0.0 1140
MS.gene052097.t1 MTR_2g097390 81.392 661 96 5 1 652 1 643 0.0 1073
MS.gene052097.t1 MTR_2g097350 80.365 657 109 5 1 652 1 642 0.0 1051
MS.gene052097.t1 MTR_2g097380 79.192 495 54 5 163 652 113 563 0.0 793
MS.gene052097.t1 MTR_4g064160 47.429 700 283 14 21 652 4 686 0.0 580
MS.gene052097.t1 MTR_2g097410 40.607 692 317 18 46 652 61 743 2.80e-158 473
MS.gene052097.t1 MTR_4g064120 42.122 622 324 14 46 652 28 628 4.21e-157 466
MS.gene052097.t1 MTR_4g064180 41.413 623 327 14 46 652 28 628 1.23e-153 457
MS.gene052097.t1 MTR_4g064150 49.778 450 203 9 222 652 290 735 3.83e-150 452
MS.gene052097.t1 MTR_7g062120 50.508 394 189 3 263 652 351 742 1.14e-147 446
MS.gene052097.t1 MTR_2g097467 38.474 642 320 15 50 652 51 656 1.75e-143 432
MS.gene052097.t1 MTR_4g064200 48.090 445 206 6 228 652 213 652 4.05e-143 431
MS.gene052097.t1 MTR_2g097463 38.548 620 343 15 54 652 55 657 2.41e-141 427
MS.gene052097.t1 MTR_2g097473 38.750 640 321 16 50 652 51 656 2.90e-140 424
MS.gene052097.t1 MTR_5g097480 30.133 375 253 4 280 649 173 543 9.71e-59 207
MS.gene052097.t1 MTR_3g056110 29.087 526 327 16 149 649 37 541 1.64e-56 201
MS.gene052097.t1 MTR_2g026250 29.765 383 258 5 275 649 216 595 2.89e-56 201
MS.gene052097.t1 MTR_4g133660 30.159 378 254 6 279 650 170 543 1.10e-55 198
MS.gene052097.t1 MTR_2g082090 30.481 374 253 3 280 649 208 578 1.22e-55 199
MS.gene052097.t1 MTR_7g027190 30.179 391 240 7 279 649 295 672 1.50e-54 198
MS.gene052097.t1 MTR_3g089055 29.459 370 254 3 284 649 198 564 3.50e-52 189
MS.gene052097.t1 MTR_3g089055 29.459 370 254 3 284 649 203 569 3.66e-52 189
MS.gene052097.t1 MTR_5g094450 29.921 381 250 8 280 649 157 531 8.68e-52 187
MS.gene052097.t1 MTR_1g029420 29.189 370 255 3 284 649 225 591 4.47e-51 187
MS.gene052097.t1 MTR_3g065980 29.847 392 245 8 274 649 162 539 3.76e-47 175
MS.gene052097.t1 MTR_8g442410 27.206 408 266 9 261 649 129 524 1.93e-46 172
MS.gene052097.t1 MTR_7g074650 30.978 368 234 9 288 651 442 793 1.51e-45 174
MS.gene052097.t1 MTR_4g104020 26.702 382 259 8 281 649 146 519 5.99e-44 165
MS.gene052097.t1 MTR_4g102790 25.521 384 266 8 280 651 288 663 2.98e-42 163
MS.gene052097.t1 MTR_5g009080 25.624 441 262 9 270 649 42 477 1.95e-40 154
MS.gene052097.t1 MTR_3g022830 25.776 419 248 8 285 649 26 435 4.20e-38 147
MS.gene052097.t1 MTR_4g097080 25.773 388 252 10 284 651 133 504 9.45e-36 141
MS.gene052097.t1 MTR_2g089100 27.404 416 239 11 281 652 49 445 2.84e-34 136
MS.gene052097.t1 MTR_2g034260 23.969 388 275 6 276 649 202 583 4.89e-34 137
MS.gene052097.t1 MTR_1g069725 28.421 380 250 11 276 650 101 463 8.15e-34 135
MS.gene052097.t1 MTR_2g034250 25.258 388 271 7 276 649 202 584 1.42e-33 136
MS.gene052097.t1 MTR_4g077760 26.882 372 246 9 289 649 198 554 7.41e-33 134
MS.gene052097.t1 MTR_2g034280 24.093 386 275 7 276 649 195 574 2.27e-30 126
MS.gene052097.t1 MTR_1g096030 27.204 397 240 14 284 649 80 458 5.76e-30 124
MS.gene052097.t1 MTR_7g109580 25.330 379 266 9 279 649 184 553 3.41e-29 122
MS.gene052097.t1 MTR_5g015490 24.611 386 265 11 284 651 114 491 4.47e-29 121
MS.gene052097.t1 MTR_4g076020 27.005 374 234 12 288 652 88 431 1.91e-28 119
MS.gene052097.t1 MTR_4g076140 27.099 262 176 5 269 523 33 286 2.80e-27 116
MS.gene052097.t1 MTR_7g057230 26.389 288 201 5 369 650 375 657 3.98e-26 114
MS.gene052097.t1 MTR_6g047750 25.199 377 259 8 285 649 296 661 2.31e-24 108
MS.gene052097.t1 MTR_1g086970 25.000 392 258 15 279 649 101 477 4.61e-24 106
MS.gene052097.t1 MTR_4g095500 23.278 421 268 11 281 652 56 470 1.23e-22 102
MS.gene052097.t1 MTR_2g099110 26.276 392 256 11 282 651 72 452 1.43e-22 101
MS.gene052097.t1 MTR_3g072710 23.940 401 261 13 279 650 115 500 2.12e-22 101
MS.gene052097.t1 MTR_5g058860 24.408 422 280 13 249 650 95 497 3.05e-22 101
MS.gene052097.t1 MTR_5g015950 21.362 426 272 10 281 652 32 448 8.19e-22 99.4
MS.gene052097.t1 MTR_3g053270 21.362 426 272 10 281 652 32 448 8.19e-22 99.4
MS.gene052097.t1 MTR_8g093070 24.010 404 264 16 270 652 117 498 3.46e-19 91.7
MS.gene052097.t1 MTR_4g026485 23.720 371 252 12 289 649 274 623 3.62e-16 82.8
MS.gene052097.t1 MTR_0092s0100 23.885 381 248 12 285 649 373 727 4.35e-16 82.4
MS.gene052097.t1 MTR_0092s0100 23.885 381 248 12 285 649 376 730 4.89e-16 82.4
MS.gene052097.t1 MTR_2g097370 90.323 31 3 0 62 92 1 31 5.17e-13 65.1
MS.gene052097.t1 MTR_8g020840 23.529 408 239 16 284 649 171 547 3.48e-11 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052097.t1 AT2G29060 41.961 622 319 10 50 651 57 656 4.84e-164 485
MS.gene052097.t1 AT2G29060 41.406 640 334 10 50 651 57 693 9.39e-164 486
MS.gene052097.t1 AT1G07530 51.282 468 204 9 204 652 305 767 1.65e-161 483
MS.gene052097.t1 AT2G29065 42.130 629 306 19 50 652 34 630 1.41e-142 429
MS.gene052097.t1 AT1G07520 48.989 445 206 10 222 652 94 531 2.23e-142 425
MS.gene052097.t1 AT1G07520 48.989 445 206 10 222 652 94 531 2.23e-142 425
MS.gene052097.t1 AT1G07520 48.989 445 206 10 222 652 259 696 6.47e-141 427
MS.gene052097.t1 AT1G07520 48.989 445 206 10 222 652 258 695 6.64e-141 427
MS.gene052097.t1 AT2G37650 47.368 380 192 4 276 651 327 702 1.00e-123 383
MS.gene052097.t1 AT2G37650 47.368 380 192 4 276 651 339 714 1.17e-123 383
MS.gene052097.t1 AT3G46600 41.972 436 241 6 222 652 121 549 2.84e-114 353
MS.gene052097.t1 AT3G46600 41.972 436 241 6 222 652 153 581 5.28e-114 354
MS.gene052097.t1 AT5G59450 40.090 444 254 5 220 652 158 600 1.67e-113 353
MS.gene052097.t1 AT3G46600 44.681 376 201 4 281 652 1 373 5.79e-112 342
MS.gene052097.t1 AT5G48150 31.633 392 260 5 263 649 101 489 1.17e-60 211
MS.gene052097.t1 AT5G48150 31.633 392 260 5 263 649 101 489 1.17e-60 211
MS.gene052097.t1 AT5G48150 31.633 392 260 5 263 649 101 489 1.17e-60 211
MS.gene052097.t1 AT5G48150 31.633 392 260 5 263 649 101 489 1.17e-60 211
MS.gene052097.t1 AT2G04890 30.811 370 250 3 280 649 49 412 4.23e-60 207
MS.gene052097.t1 AT1G50600 30.941 404 255 6 264 649 128 525 1.02e-58 206
MS.gene052097.t1 AT1G50600 30.941 404 255 6 264 649 199 596 1.53e-58 207
MS.gene052097.t1 AT1G50600 30.941 404 255 6 264 649 203 600 2.10e-58 207
MS.gene052097.t1 AT1G21450 29.199 387 257 8 274 649 212 592 1.09e-49 183
MS.gene052097.t1 AT3G54220 31.328 399 251 10 260 651 269 651 1.06e-47 178
MS.gene052097.t1 AT2G01570 29.534 386 243 10 274 649 214 580 2.68e-47 176
MS.gene052097.t1 AT1G66350 30.570 386 233 11 274 649 145 505 2.68e-47 174
MS.gene052097.t1 AT3G03450 27.792 385 256 8 274 649 173 544 2.37e-46 172
MS.gene052097.t1 AT1G14920 28.272 382 253 7 274 649 162 528 5.41e-46 171
MS.gene052097.t1 AT4G17230 28.117 377 264 3 279 651 153 526 2.59e-45 169
MS.gene052097.t1 AT4G17230 28.117 377 264 3 279 651 153 526 2.59e-45 169
MS.gene052097.t1 AT5G17490 25.995 377 263 7 276 649 152 515 1.69e-43 164
MS.gene052097.t1 AT5G66770 29.679 374 237 11 288 649 224 583 2.71e-36 144
MS.gene052097.t1 AT1G50420 22.718 471 291 11 246 649 14 478 1.51e-35 140
MS.gene052097.t1 AT1G63100 28.531 354 232 9 280 624 248 589 7.95e-35 140
MS.gene052097.t1 AT1G63100 28.531 354 232 9 280 624 273 614 8.40e-35 140
MS.gene052097.t1 AT5G41920 29.716 387 231 15 279 649 38 399 2.05e-34 135
MS.gene052097.t1 AT4G37650 25.556 360 234 9 283 617 145 495 9.58e-33 133
MS.gene052097.t1 AT3G50650 27.147 361 234 9 304 649 195 541 1.71e-31 129
MS.gene052097.t1 AT3G49950 25.765 392 261 9 281 651 27 409 3.70e-28 117
MS.gene052097.t1 AT4G08250 25.762 361 242 12 303 649 128 476 5.41e-26 112
MS.gene052097.t1 AT1G55580 24.760 416 254 12 281 649 41 444 4.64e-25 108
MS.gene052097.t1 AT5G52510 23.834 386 258 9 285 649 269 639 4.00e-24 107
MS.gene052097.t1 AT3G13840 23.499 383 255 11 284 649 148 509 5.17e-13 72.4

Find 109 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAATCTGATTTAATATT+TGG 0.081879 2.3:+5614283 None:intergenic
TTCTATTTGGTTCTTCTTGC+TGG 0.149493 2.3:-5613261 MS.gene052097:CDS
TTCACTCTCTCCATTGAATT+TGG 0.151096 2.3:+5613907 None:intergenic
AGTCTTCACATTATAGATTT+TGG 0.160984 2.3:-5614024 MS.gene052097:CDS
AATCTTCTTCCATGAGAAAT+TGG 0.197500 2.3:+5615012 None:intergenic
TATCCCTGTGATCTTCAATT+TGG 0.207790 2.3:+5613936 None:intergenic
GAATTCTCTTCAACTGTTCT+TGG 0.222104 2.3:-5615140 MS.gene052097:CDS
TGGGTTTAAGTCTTTGAAAT+TGG 0.243482 2.3:-5613395 MS.gene052097:CDS
TTTATTCATAGCATTGTTAA+TGG 0.246687 2.3:-5613649 MS.gene052097:CDS
CAAAGATTGGCACATTTCTA+TGG 0.249724 2.3:-5614231 MS.gene052097:CDS
GAATCCGAAGGGTGCTGTTT+TGG 0.259645 2.3:-5613701 MS.gene052097:CDS
TGAAATATCATTACAAGAAA+TGG 0.276552 2.3:+5614314 None:intergenic
TTATTTGATAAATTACTTCT+TGG 0.276677 2.3:-5614480 MS.gene052097:CDS
CATATAGGCATTGGCATTCT+AGG 0.286084 2.3:-5613429 MS.gene052097:CDS
CCAAATATTAAATCAGATTA+AGG 0.286177 2.3:-5614283 MS.gene052097:CDS
TAGTTGTATGAGTCTATAAT+TGG 0.294656 2.3:+5614702 None:intergenic
ATTCACTCTTACTTTGTTCA+TGG 0.301028 2.3:+5615159 None:intergenic
TGATGTTTGAGGAAGTGTTT+TGG 0.302555 2.3:-5613513 MS.gene052097:CDS
TGTTTGAGGTGAAGGAAAAT+TGG 0.305166 2.3:-5613309 MS.gene052097:CDS
TGGAAGGGTAGAATTCTATT+TGG 0.313548 2.3:-5613274 MS.gene052097:CDS
GGAGGGAATGGAAGAGTAGT+TGG 0.316336 2.3:+5614657 None:intergenic
CATTCTAGGCACGTGGGAAA+TGG 0.320991 2.3:-5613415 MS.gene052097:CDS
ATCCCTGTGATCTTCAATTT+GGG 0.345121 2.3:+5613937 None:intergenic
AGAGTAGTTGGGAGTAGAAA+AGG 0.358921 2.3:+5614669 None:intergenic
TCCTCAAACATCAACCTATT+AGG 0.361937 2.3:+5613523 None:intergenic
GAGTGAATGAAGCAGGTCTT+AGG 0.373688 2.3:-5613891 MS.gene052097:CDS
TCTATTTGGTTCTTCTTGCT+GGG 0.374895 2.3:-5613260 MS.gene052097:CDS
CATTATTGAAAGAAGGGAAA+AGG 0.380669 2.3:+5614604 None:intergenic
ATGCTATGAATAAATATATT+CGG 0.399119 2.3:+5613658 None:intergenic
GCAATACCATTGTACTCAAA+AGG 0.401991 2.3:+5613838 None:intergenic
GTTGAAGAGCATAGGAATCT+TGG 0.409119 2.3:+5614967 None:intergenic
TTGAGAGGCCAGAGACATAT+AGG 0.413255 2.3:-5613444 MS.gene052097:CDS
AGAAGGGAAAAGGGTTGGAT+TGG 0.418599 2.3:+5614614 None:intergenic
TATAATTGGATCAAGTTCAT+AGG 0.422425 2.3:+5614716 None:intergenic
GAAAGAGCATTATTGAAAGA+AGG 0.427798 2.3:+5614597 None:intergenic
GTGAACGTGAGGATCCTAAT+AGG 0.434511 2.3:-5613537 MS.gene052097:CDS
ACTCTTCATGTTCATTGTGT+TGG 0.439658 2.3:+5615060 None:intergenic
AAGGAACATTCTTCTCCAAC+AGG 0.441908 2.3:-5614264 MS.gene052097:CDS
AAAGAGCATTATTGAAAGAA+GGG 0.443472 2.3:+5614598 None:intergenic
ACTCGATGAGACAGTAGTTT+CGG 0.444774 2.3:-5613725 MS.gene052097:CDS
AGACGAAGAAGAGGAAGAAG+AGG 0.446816 2.3:+5614857 None:intergenic
TTGAAAGAAGGGAAAAGGGT+TGG 0.454471 2.3:+5614609 None:intergenic
CTTTGAGTTCCATCACCTGT+TGG 0.456885 2.3:+5614249 None:intergenic
GATAGATCTTCCAAATTCAA+TGG 0.460382 2.3:-5613917 MS.gene052097:CDS
CTTGGTATTAAAGTCGCAAA+AGG 0.460997 2.3:-5614462 MS.gene052097:CDS
TAGGAGAAAGAGAAGTTATG+AGG 0.463600 2.3:-5614379 MS.gene052097:CDS
ATTCTAGGCACGTGGGAAAT+GGG 0.464304 2.3:-5613414 MS.gene052097:CDS
AGAATAAGAAGACGAAGAAG+AGG 0.467187 2.3:+5614848 None:intergenic
GAGTAGTTGGGAGTAGAAAA+GGG 0.473309 2.3:+5614670 None:intergenic
GAAGCACGTTTAGCAGGAAC+TGG 0.473702 2.3:-5614198 MS.gene052097:CDS
TCCTAATAGGTTGATGTTTG+AGG 0.474185 2.3:-5613524 MS.gene052097:CDS
GAGGGAATGGAAGAGTAGTT+GGG 0.474502 2.3:+5614658 None:intergenic
ATTATTGAAAGAAGGGAAAA+GGG 0.478667 2.3:+5614605 None:intergenic
GAAGAGTCTTCCAATGCTCT+TGG 0.479029 2.3:-5615044 MS.gene052097:CDS
GAGAAGTTATGAGGAAGTTG+TGG 0.483254 2.3:-5614370 MS.gene052097:CDS
TGCCAATGCCTATATGTCTC+TGG 0.483967 2.3:+5613436 None:intergenic
TAGCCTCTGAAGGTTGTGAC+AGG 0.486539 2.3:-5613468 MS.gene052097:CDS
GCAGTAGCAGCAGTAGGAGG+AGG 0.489711 2.3:+5614909 None:intergenic
GCAGATTATAGATAAATTGA+AGG 0.495433 2.3:-5613368 MS.gene052097:CDS
GACGAAGAAGAGGAAGAAGA+GGG 0.495930 2.3:+5614858 None:intergenic
GCAGCAGCAGTAGCAGCAGT+AGG 0.498059 2.3:+5614903 None:intergenic
GAAGAATTATCAAACAGATT+CGG 0.502364 2.3:-5614408 MS.gene052097:CDS
AAATTATAATCACTGAAGCT+AGG 0.502389 2.3:+5614759 None:intergenic
CCACCCAAATTGAAGATCAC+AGG 0.507118 2.3:-5613940 MS.gene052097:CDS
AAAATTGGATGCTACAAGGT+TGG 0.509330 2.3:-5613294 MS.gene052097:CDS
AAAATGTGATTAGGAGGGAA+TGG 0.510292 2.3:+5614645 None:intergenic
CTTCAATTTGGGTGGCCCAC+CGG 0.513811 2.3:+5613948 None:intergenic
CATATTGTGAAGGTCTATGC+TGG 0.514011 2.3:+5613235 None:intergenic
GTTGATATAACCAAGAGCAT+TGG 0.514520 2.3:+5615034 None:intergenic
CATTCTTCTCCAACAGGTGA+TGG 0.515669 2.3:-5614258 MS.gene052097:CDS
GCACTCGAAGCACGTTTAGC+AGG 0.522176 2.3:-5614204 MS.gene052097:CDS
GGCCAGAGACATATAGGCAT+TGG 0.526736 2.3:-5613438 MS.gene052097:CDS
GGAAGAAGAGGGAAGAGTAG+TGG 0.529525 2.3:+5614869 None:intergenic
TTATATCAACCAATTTCTCA+TGG 0.530130 2.3:-5615021 MS.gene052097:CDS
AAATCCAAAACAGCACCCTT+CGG 0.534191 2.3:+5613697 None:intergenic
CACCGTCTATCGAAAAGATC+CGG 0.539937 2.3:-5613967 MS.gene052097:CDS
CAATAATGCTCTTTCTCACG+AGG 0.541791 2.3:-5614589 MS.gene052097:CDS
GGCATGCTGATGAATAAACT+TGG 0.547453 2.3:+5614112 None:intergenic
TTGGATGCTACAAGGTTGGA+AGG 0.548278 2.3:-5613290 MS.gene052097:CDS
TTGGGAAAGTATCAAAGTTG+AGG 0.550259 2.3:-5613809 MS.gene052097:CDS
CAGATTATAGATAAATTGAA+GGG 0.550818 2.3:-5613367 MS.gene052097:CDS
ATGGAGAGAGTGAATGAAGC+AGG 0.553640 2.3:-5613898 MS.gene052097:CDS
TTATAGATAAATTGAAGGGT+AGG 0.554979 2.3:-5613363 MS.gene052097:CDS
AGGTGATGGAACTCAAAGAT+TGG 0.559955 2.3:-5614244 MS.gene052097:CDS
AATTATAATCACTGAAGCTA+GGG 0.560160 2.3:+5614760 None:intergenic
AACCCTGTCACAACCTTCAG+AGG 0.560545 2.3:+5613465 None:intergenic
CCTGTGATCTTCAATTTGGG+TGG 0.565831 2.3:+5613940 None:intergenic
GATAGACGGTGTATAAAAGC+AGG 0.571505 2.3:+5613979 None:intergenic
GCAGCAGTAGCAGCAGTAGG+AGG 0.573244 2.3:+5614906 None:intergenic
AGTGATTATAATTTCAGCAG+TGG 0.578633 2.3:-5614750 MS.gene052097:CDS
GAATTATCAAACAGATTCGG+TGG 0.598197 2.3:-5614405 MS.gene052097:CDS
CACCCAAATTGAAGATCACA+GGG 0.598988 2.3:-5613939 MS.gene052097:CDS
CTCAGTAAGTTGAAGAGCAT+AGG 0.600415 2.3:+5614959 None:intergenic
AAGGTTGTGACAGGGTTGAG+AGG 0.603768 2.3:-5613459 MS.gene052097:CDS
ATGAATGTTATAGCCTCTGA+AGG 0.605131 2.3:-5613478 MS.gene052097:CDS
CAGTAGCAGCAGTAGGAGGA+GGG 0.605945 2.3:+5614910 None:intergenic
AAGGAAAATTGGATGCTACA+AGG 0.606455 2.3:-5613298 MS.gene052097:CDS
GTTATCAAGCGTATCAAACA+AGG 0.610647 2.3:+5613567 None:intergenic
GTGTTGGTTATGGTTTCAAG+TGG 0.612035 2.3:-5614002 MS.gene052097:CDS
AGTAGTTTCGGAGAATCCGA+AGG 0.616125 2.3:-5613713 MS.gene052097:CDS
ATTCATAGCATTGTTAATGG+CGG 0.619293 2.3:-5613646 MS.gene052097:CDS
CGTCTATCGAAAAGATCCGG+TGG 0.631429 2.3:-5613964 MS.gene052097:CDS
GTCTATCGAAAAGATCCGGT+GGG 0.632717 2.3:-5613963 MS.gene052097:CDS
AGCCTCTGAAGGTTGTGACA+GGG 0.632967 2.3:-5613467 MS.gene052097:CDS
TGGATGCTACAAGGTTGGAA+GGG 0.641743 2.3:-5613289 MS.gene052097:CDS
GCATTGGCATTCTAGGCACG+TGG 0.643333 2.3:-5613422 MS.gene052097:CDS
TAACAACAATAGTGAACGTG+AGG 0.667397 2.3:-5613548 MS.gene052097:CDS
CATTGGCATTCTAGGCACGT+GGG 0.682240 2.3:-5613421 MS.gene052097:CDS
GTAGTTTCGGAGAATCCGAA+GGG 0.706195 2.3:-5613712 MS.gene052097:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCTATGAATAAATATATT+CGG + chr2.3:5614779-5614798 None:intergenic 15.0%
!! TTATTTGATAAATTACTTCT+TGG - chr2.3:5613954-5613973 MS.gene052097:CDS 15.0%
!!! TTTTATAAACAAATTTCTCA+TGG - chr2.3:5613317-5613336 MS.gene052097:CDS 15.0%
!! CAGATTATAGATAAATTGAA+GGG - chr2.3:5615067-5615086 MS.gene052097:CDS 20.0%
!! CCAAATATTAAATCAGATTA+AGG - chr2.3:5614151-5614170 MS.gene052097:CDS 20.0%
!! CCTTAATCTGATTTAATATT+TGG + chr2.3:5614154-5614173 None:intergenic 20.0%
!! TGAAATATCATTACAAGAAA+TGG + chr2.3:5614123-5614142 None:intergenic 20.0%
!! TTTATTCATAGCATTGTTAA+TGG - chr2.3:5614785-5614804 MS.gene052097:CDS 20.0%
! AAATTATAATCACTGAAGCT+AGG + chr2.3:5613678-5613697 None:intergenic 25.0%
! AATTATAATCACTGAAGCTA+GGG + chr2.3:5613677-5613696 None:intergenic 25.0%
! ACAATGGTATTGCAAAAAAT+TGG - chr2.3:5614606-5614625 MS.gene052097:CDS 25.0%
! ATTATTGAAAGAAGGGAAAA+GGG + chr2.3:5613832-5613851 None:intergenic 25.0%
! CAATGGTATTGCAAAAAATT+GGG - chr2.3:5614607-5614626 MS.gene052097:CDS 25.0%
! GAAGAATTATCAAACAGATT+CGG - chr2.3:5614026-5614045 MS.gene052097:CDS 25.0%
! GATTGAGAAAAAATGTGATT+AGG + chr2.3:5613801-5613820 None:intergenic 25.0%
! GCAGATTATAGATAAATTGA+AGG - chr2.3:5615066-5615085 MS.gene052097:CDS 25.0%
! TAGTTGTATGAGTCTATAAT+TGG + chr2.3:5613735-5613754 None:intergenic 25.0%
! TATAATTGGATCAAGTTCAT+AGG + chr2.3:5613721-5613740 None:intergenic 25.0%
! TTAAACCTTGTCACAAAAAA+AGG + chr2.3:5614824-5614843 None:intergenic 25.0%
! TTATAGATAAATTGAAGGGT+AGG - chr2.3:5615071-5615090 MS.gene052097:CDS 25.0%
! TTATATCAACCAATTTCTCA+TGG - chr2.3:5613413-5613432 MS.gene052097:CDS 25.0%
!! AGTCTTCACATTATAGATTT+TGG - chr2.3:5614410-5614429 MS.gene052097:CDS 25.0%
!! ATAATTGCAAGCTTTTCAAA+AGG + chr2.3:5614347-5614366 None:intergenic 25.0%
!! TAATAGTGATTTTCTGTTTG+AGG - chr2.3:5615111-5615130 MS.gene052097:CDS 25.0%
!! TAATTGCAAGCTTTTCAAAA+GGG + chr2.3:5614346-5614365 None:intergenic 25.0%
!!! AAAGAGCATTATTGAAAGAA+GGG + chr2.3:5613839-5613858 None:intergenic 25.0%
!!! AAGTTGAGGATTTTAAGATA+AGG - chr2.3:5614639-5614658 MS.gene052097:CDS 25.0%
!!! TTTTTTTGTGACAAGGTTTA+AGG - chr2.3:5614823-5614842 MS.gene052097:CDS 25.0%
AATCTTCTTCCATGAGAAAT+TGG + chr2.3:5613425-5613444 None:intergenic 30.0%
AGTGATTATAATTTCAGCAG+TGG - chr2.3:5613684-5613703 MS.gene052097:CDS 30.0%
ATTCACTCTTACTTTGTTCA+TGG + chr2.3:5613278-5613297 None:intergenic 30.0%
ATTCATAGCATTGTTAATGG+CGG - chr2.3:5614788-5614807 MS.gene052097:CDS 30.0%
CATTATTGAAAGAAGGGAAA+AGG + chr2.3:5613833-5613852 None:intergenic 30.0%
GAGAAAAAATGTGATTAGGA+GGG + chr2.3:5613797-5613816 None:intergenic 30.0%
GATAGATCTTCCAAATTCAA+TGG - chr2.3:5614517-5614536 MS.gene052097:CDS 30.0%
TGAGAAAAAATGTGATTAGG+AGG + chr2.3:5613798-5613817 None:intergenic 30.0%
!! AATGTTCCTTTTGAGTACAA+TGG - chr2.3:5614590-5614609 MS.gene052097:CDS 30.0%
!! CACATTATAGATTTTGGTGT+TGG - chr2.3:5614416-5614435 MS.gene052097:CDS 30.0%
!! GAAAGAGCATTATTGAAAGA+AGG + chr2.3:5613840-5613859 None:intergenic 30.0%
!! TGGGTTTAAGTCTTTGAAAT+TGG - chr2.3:5615039-5615058 MS.gene052097:CDS 30.0%
!!! ATAGATTTTGGTGTTGGTTA+TGG - chr2.3:5614422-5614441 MS.gene052097:CDS 30.0%
AAAATGTGATTAGGAGGGAA+TGG + chr2.3:5613792-5613811 None:intergenic 35.0%
AAAATTGGATGCTACAAGGT+TGG - chr2.3:5615140-5615159 MS.gene052097:CDS 35.0%
AAGGAAAATTGGATGCTACA+AGG - chr2.3:5615136-5615155 MS.gene052097:CDS 35.0%
ACTCTTCATGTTCATTGTGT+TGG + chr2.3:5613377-5613396 None:intergenic 35.0%
AGAATAAGAAGACGAAGAAG+AGG + chr2.3:5613589-5613608 None:intergenic 35.0%
ATCCCTGTGATCTTCAATTT+GGG + chr2.3:5614500-5614519 None:intergenic 35.0%
ATGAATGTTATAGCCTCTGA+AGG - chr2.3:5614956-5614975 MS.gene052097:CDS 35.0%
CAAAGATTGGCACATTTCTA+TGG - chr2.3:5614203-5614222 MS.gene052097:CDS 35.0%
CTTGGTATTAAAGTCGCAAA+AGG - chr2.3:5613972-5613991 MS.gene052097:CDS 35.0%
GAAAAAATCTGCTGCAGATA+TGG - chr2.3:5614289-5614308 MS.gene052097:CDS 35.0%
GAATTATCAAACAGATTCGG+TGG - chr2.3:5614029-5614048 MS.gene052097:CDS 35.0%
GAATTCTCTTCAACTGTTCT+TGG - chr2.3:5613294-5613313 MS.gene052097:CDS 35.0%
GCAATACCATTGTACTCAAA+AGG + chr2.3:5614599-5614618 None:intergenic 35.0%
GTTATCAAGCGTATCAAACA+AGG + chr2.3:5614870-5614889 None:intergenic 35.0%
TAACAACAATAGTGAACGTG+AGG - chr2.3:5614886-5614905 MS.gene052097:CDS 35.0%
TAGGAGAAAGAGAAGTTATG+AGG - chr2.3:5614055-5614074 MS.gene052097:CDS 35.0%
TATCCCTGTGATCTTCAATT+TGG + chr2.3:5614501-5614520 None:intergenic 35.0%
TCCTCAAACATCAACCTATT+AGG + chr2.3:5614914-5614933 None:intergenic 35.0%
TGGAAGGGTAGAATTCTATT+TGG - chr2.3:5615160-5615179 MS.gene052097:CDS 35.0%
TTCACTCTCTCCATTGAATT+TGG + chr2.3:5614530-5614549 None:intergenic 35.0%
! GTTGATATAACCAAGAGCAT+TGG + chr2.3:5613403-5613422 None:intergenic 35.0%
! TGTTTGAGGTGAAGGAAAAT+TGG - chr2.3:5615125-5615144 MS.gene052097:CDS 35.0%
!! TCCTAATAGGTTGATGTTTG+AGG - chr2.3:5614910-5614929 MS.gene052097:CDS 35.0%
!! TCTATTTGGTTCTTCTTGCT+GGG - chr2.3:5615174-5615193 MS.gene052097:CDS 35.0%
!! TGATTTTCTGTTTGAGGTGA+AGG - chr2.3:5615117-5615136 MS.gene052097:CDS 35.0%
!! TTCTATTTGGTTCTTCTTGC+TGG - chr2.3:5615173-5615192 MS.gene052097:CDS 35.0%
!! TTGGGAAAGTATCAAAGTTG+AGG - chr2.3:5614625-5614644 MS.gene052097:CDS 35.0%
!!! ATCTGCAGCAGATTTTTTCT+TGG + chr2.3:5614288-5614307 None:intergenic 35.0%
!!! ATGCACCTTTTTTTGTGACA+AGG - chr2.3:5614816-5614835 MS.gene052097:CDS 35.0%
!!! ATGTTTGAGGAAGTGTTTTG+GGG - chr2.3:5614923-5614942 MS.gene052097:CDS 35.0%
!!! GATGTTTGAGGAAGTGTTTT+GGG - chr2.3:5614922-5614941 MS.gene052097:CDS 35.0%
!!! TGAAAGCTTGTGTTTTGAAG+TGG + chr2.3:5613999-5614018 None:intergenic 35.0%
!!! TGATGTTTGAGGAAGTGTTT+TGG - chr2.3:5614921-5614940 MS.gene052097:CDS 35.0%
AAATCCAAAACAGCACCCTT+CGG + chr2.3:5614740-5614759 None:intergenic 40.0%
AAGGAACATTCTTCTCCAAC+AGG - chr2.3:5614170-5614189 MS.gene052097:CDS 40.0%
ACTCGATGAGACAGTAGTTT+CGG - chr2.3:5614709-5614728 MS.gene052097:CDS 40.0%
AGAGTAGTTGGGAGTAGAAA+AGG + chr2.3:5613768-5613787 None:intergenic 40.0%
CAATAATGCTCTTTCTCACG+AGG - chr2.3:5613845-5613864 MS.gene052097:CDS 40.0%
CACCCAAATTGAAGATCACA+GGG - chr2.3:5614495-5614514 MS.gene052097:CDS 40.0%
GAGAAGTTATGAGGAAGTTG+TGG - chr2.3:5614064-5614083 MS.gene052097:CDS 40.0%
GAGTAGTTGGGAGTAGAAAA+GGG + chr2.3:5613767-5613786 None:intergenic 40.0%
GATAGACGGTGTATAAAAGC+AGG + chr2.3:5614458-5614477 None:intergenic 40.0%
GGCATGCTGATGAATAAACT+TGG + chr2.3:5614325-5614344 None:intergenic 40.0%
TTGAAAGAAGGGAAAAGGGT+TGG + chr2.3:5613828-5613847 None:intergenic 40.0%
! CTCAGTAAGTTGAAGAGCAT+AGG + chr2.3:5613478-5613497 None:intergenic 40.0%
! GTTGAAGAGCATAGGAATCT+TGG + chr2.3:5613470-5613489 None:intergenic 40.0%
!! AGGTGATGGAACTCAAAGAT+TGG - chr2.3:5614190-5614209 MS.gene052097:CDS 40.0%
!! AGTTGTAGTTTTGCTGATGC+TGG + chr2.3:5613644-5613663 None:intergenic 40.0%
!! CATATAGGCATTGGCATTCT+AGG - chr2.3:5615005-5615024 MS.gene052097:CDS 40.0%
!! GTGTTGGTTATGGTTTCAAG+TGG - chr2.3:5614432-5614451 MS.gene052097:CDS 40.0%
!!! CAAACAGATTCGGTGGTTTT+AGG - chr2.3:5614036-5614055 MS.gene052097:CDS 40.0%
!!! TGTTTGAGGAAGTGTTTTGG+GGG - chr2.3:5614924-5614943 MS.gene052097:CDS 40.0%
AGAAGGGAAAAGGGTTGGAT+TGG + chr2.3:5613823-5613842 None:intergenic 45.0%
AGACGAAGAAGAGGAAGAAG+AGG + chr2.3:5613580-5613599 None:intergenic 45.0%
AGTAGTTTCGGAGAATCCGA+AGG - chr2.3:5614721-5614740 MS.gene052097:CDS 45.0%
ATGGAGAGAGTGAATGAAGC+AGG - chr2.3:5614536-5614555 MS.gene052097:CDS 45.0%
ATTCTAGGCACGTGGGAAAT+GGG - chr2.3:5615020-5615039 MS.gene052097:CDS 45.0%
CACCGTCTATCGAAAAGATC+CGG - chr2.3:5614467-5614486 MS.gene052097:CDS 45.0%
CATTCTTCTCCAACAGGTGA+TGG - chr2.3:5614176-5614195 MS.gene052097:CDS 45.0%
CCACCCAAATTGAAGATCAC+AGG - chr2.3:5614494-5614513 MS.gene052097:CDS 45.0%
CCTGTGATCTTCAATTTGGG+TGG + chr2.3:5614497-5614516 None:intergenic 45.0%
GAAGAGTCTTCCAATGCTCT+TGG - chr2.3:5613390-5613409 MS.gene052097:CDS 45.0%
GACGAAGAAGAGGAAGAAGA+GGG + chr2.3:5613579-5613598 None:intergenic 45.0%
GAGGGAATGGAAGAGTAGTT+GGG + chr2.3:5613779-5613798 None:intergenic 45.0%
GAGTGAATGAAGCAGGTCTT+AGG - chr2.3:5614543-5614562 MS.gene052097:CDS 45.0%
GTAGTTTCGGAGAATCCGAA+GGG - chr2.3:5614722-5614741 MS.gene052097:CDS 45.0%
GTCTATCGAAAAGATCCGGT+GGG - chr2.3:5614471-5614490 MS.gene052097:CDS 45.0%
GTGAACGTGAGGATCCTAAT+AGG - chr2.3:5614897-5614916 MS.gene052097:CDS 45.0%
TGCCAATGCCTATATGTCTC+TGG + chr2.3:5615001-5615020 None:intergenic 45.0%
TGGATGCTACAAGGTTGGAA+GGG - chr2.3:5615145-5615164 MS.gene052097:CDS 45.0%
TTGAGAGGCCAGAGACATAT+AGG - chr2.3:5614990-5615009 MS.gene052097:CDS 45.0%
TTGGATGCTACAAGGTTGGA+AGG - chr2.3:5615144-5615163 MS.gene052097:CDS 45.0%
! CACCGGATCTTTTCGATAGA+CGG + chr2.3:5614472-5614491 None:intergenic 45.0%
! CTTTGAGTTCCATCACCTGT+TGG + chr2.3:5614188-5614207 None:intergenic 45.0%
!! GTAGTTTTGCTGATGCTGGA+TGG + chr2.3:5613640-5613659 None:intergenic 45.0%
AACCCTGTCACAACCTTCAG+AGG + chr2.3:5614972-5614991 None:intergenic 50.0%
AAGGTTGTGACAGGGTTGAG+AGG - chr2.3:5614975-5614994 MS.gene052097:CDS 50.0%
CATTCTAGGCACGTGGGAAA+TGG - chr2.3:5615019-5615038 MS.gene052097:CDS 50.0%
CATTGGCATTCTAGGCACGT+GGG - chr2.3:5615013-5615032 MS.gene052097:CDS 50.0%
CGTCTATCGAAAAGATCCGG+TGG - chr2.3:5614470-5614489 MS.gene052097:CDS 50.0%
GAAGCACGTTTAGCAGGAAC+TGG - chr2.3:5614236-5614255 MS.gene052097:CDS 50.0%
GGAAGAAGAGGGAAGAGTAG+TGG + chr2.3:5613568-5613587 None:intergenic 50.0%
GGAGGGAATGGAAGAGTAGT+TGG + chr2.3:5613780-5613799 None:intergenic 50.0%
GGCCAGAGACATATAGGCAT+TGG - chr2.3:5614996-5615015 MS.gene052097:CDS 50.0%
! AGCCTCTGAAGGTTGTGACA+GGG - chr2.3:5614967-5614986 MS.gene052097:CDS 50.0%
! TAGCCTCTGAAGGTTGTGAC+AGG - chr2.3:5614966-5614985 MS.gene052097:CDS 50.0%
!!! GAATCCGAAGGGTGCTGTTT+TGG - chr2.3:5614733-5614752 MS.gene052097:CDS 50.0%
CAGTAGCAGCAGTAGGAGGA+GGG + chr2.3:5613527-5613546 None:intergenic 55.0%
CTTCAATTTGGGTGGCCCAC+CGG + chr2.3:5614489-5614508 None:intergenic 55.0%
GCACTCGAAGCACGTTTAGC+AGG - chr2.3:5614230-5614249 MS.gene052097:CDS 55.0%
! GCATTGGCATTCTAGGCACG+TGG - chr2.3:5615012-5615031 MS.gene052097:CDS 55.0%
GCAGCAGCAGTAGCAGCAGT+AGG + chr2.3:5613534-5613553 None:intergenic 60.0%
GCAGCAGTAGCAGCAGTAGG+AGG + chr2.3:5613531-5613550 None:intergenic 60.0%
GCAGTAGCAGCAGTAGGAGG+AGG + chr2.3:5613528-5613547 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 5613249 5615207 5613249 ID=MS.gene052097
chr2.3 mRNA 5613249 5615207 5613249 ID=MS.gene052097.t1;Parent=MS.gene052097
chr2.3 exon 5613249 5615207 5613249 ID=MS.gene052097.t1.exon1;Parent=MS.gene052097.t1
chr2.3 CDS 5613249 5615207 5613249 ID=cds.MS.gene052097.t1;Parent=MS.gene052097.t1
Gene Sequence

>MS.gene052097

ATGGACAAAAGAGTTGTTGATTCACACCATGAACAAAGTAAGAGTGAATTCTCTTCAACTGTTCTTGGTTTTATAAACAAATTTCTCATGGAAGATGATGACATAGAAGAAGACTACAATATGTTCCAACACAATGAACATGAAGAGTCTTCCAATGCTCTTGGTTATATCAACCAATTTCTCATGGAAGAAGATTTTGAAAAAGAATACACTATGTTCCAAGATTCCTATGCTCTTCAACTTACTGAGAAATCATTTCATGATGTTATTTCTCACCCTCCTCCTACTGCTGCTACTGCTGCTGCTGCTGCTGCTGCCACTACTCTTCCCTCTTCTTCCTCTTCTTCGTCTTCTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCATCCAGCATCAGCAAAACTACAACTATGTGCATAGCCCTAGCTTCAGTGATTATAATTTCAGCAGTGGTAGCACTAGCTCCTATGAACTTGATCCAATTATAGACTCATACAACTATAATAACAACCCTTTTCTACTCCCAACTACTCTTCCATTCCCTCCTAATCACATTTTTTCTCAATCCAATCCAACCCTTTTCCCTTCTTTCAATAATGCTCTTTCTCACGAGGTAATTAAGACAGAAAACTTTGAACAAGAGCATTTTCTTAATGTGTCAAAACAAGTGAATGTACATGATAACAGTGAGTTATCAGAATTATTTGATAAATTACTTCTTGGTATTAAAGTCGCAAAAGGTCCACTTCAAAACACAAGCTTTCAGCAGAATGAAGAATTATCAAACAGATTCGGTGGTTTTAGGAGAAAGAGAAGTTATGAGGAAGTTGTGGATTTGAGCACACTATTGATGCTATGTGCACAATCCATTTCTTGTAATGATATTTCAAATGCTAACCAAATATTAAATCAGATTAAGGAACATTCTTCTCCAACAGGTGATGGAACTCAAAGATTGGCACATTTCTATGGAAATGCACTCGAAGCACGTTTAGCAGGAACTGGTTCACTTATTTATAGAGCTCTATCTTCCAAGAAAAAATCTGCTGCAGATATGGCAAGAGCTTACCAAGTTTATTCATCAGCATGCCCTTTTGAAAAGCTTGCAATTATGTTCTCAAACAATGCAATTTTCAATGTAGCAAAAGAAACAGAAAGTCTTCACATTATAGATTTTGGTGTTGGTTATGGTTTCAAGTGGCCTGCTTTTATACACCGTCTATCGAAAAGATCCGGTGGGCCACCCAAATTGAAGATCACAGGGATAGATCTTCCAAATTCAATGGAGAGAGTGAATGAAGCAGGTCTTAGGCTTGCAAGTTATTGCAAACGTTTCAATGTTCCTTTTGAGTACAATGGTATTGCAAAAAATTGGGAAAGTATCAAAGTTGAGGATTTTAAGATAAGGAAAAATGAGTTTGTTGCTGTGAATTGTTTGTTTAAGTTTGAGAATCTACTCGATGAGACAGTAGTTTCGGAGAATCCGAAGGGTGCTGTTTTGGATTTGATTAGAAAGACAAATCCGAATATATTTATTCATAGCATTGTTAATGGCGGTTATGATGCACCTTTTTTTGTGACAAGGTTTAAGGAAGCTGTTTTTCATTATTCAGCCTTGTTTGATACGCTTGATAACAACAATAGTGAACGTGAGGATCCTAATAGGTTGATGTTTGAGGAAGTGTTTTGGGGGAAAGATATAATGAATGTTATAGCCTCTGAAGGTTGTGACAGGGTTGAGAGGCCAGAGACATATAGGCATTGGCATTCTAGGCACGTGGGAAATGGGTTTAAGTCTTTGAAATTGGATAAGCAGATTATAGATAAATTGAAGGGTAGGTTGAGAGATGATGCATATAATAGTGATTTTCTGTTTGAGGTGAAGGAAAATTGGATGCTACAAGGTTGGAAGGGTAGAATTCTATTTGGTTCTTCTTGCTGGGTTCCAGCATAG
Protein sequence

>MS.gene052097.t1

MDKRVVDSHHEQSKSEFSSTVLGFINKFLMEDDDIEEDYNMFQHNEHEESSNALGYINQFLMEEDFEKEYTMFQDSYALQLTEKSFHDVISHPPPTAATAAAAAAATTLPSSSSSSSSYSSSSSSSSSSSIQHQQNYNYVHSPSFSDYNFSSGSTSSYELDPIIDSYNYNNNPFLLPTTLPFPPNHIFSQSNPTLFPSFNNALSHEVIKTENFEQEHFLNVSKQVNVHDNSELSELFDKLLLGIKVAKGPLQNTSFQQNEELSNRFGGFRRKRSYEEVVDLSTLLMLCAQSISCNDISNANQILNQIKEHSSPTGDGTQRLAHFYGNALEARLAGTGSLIYRALSSKKKSAADMARAYQVYSSACPFEKLAIMFSNNAIFNVAKETESLHIIDFGVGYGFKWPAFIHRLSKRSGGPPKLKITGIDLPNSMERVNEAGLRLASYCKRFNVPFEYNGIAKNWESIKVEDFKIRKNEFVAVNCLFKFENLLDETVVSENPKGAVLDLIRKTNPNIFIHSIVNGGYDAPFFVTRFKEAVFHYSALFDTLDNNNSEREDPNRLMFEEVFWGKDIMNVIASEGCDRVERPETYRHWHSRHVGNGFKSLKLDKQIIDKLKGRLRDDAYNSDFLFEVKENWMLQGWKGRILFGSSCWVPA