AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052196

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052196.t1 MTR_2g093960 35.393 178 99 5 104 272 37 207 1.37e-27 111
MS.gene052196.t1 MTR_8g017210 35.897 156 92 3 104 259 67 214 9.91e-27 110
MS.gene052196.t1 MTR_4g059870 35.897 156 93 4 104 259 43 191 4.10e-26 108
MS.gene052196.t1 MTR_2g099990 35.256 156 93 3 104 259 51 198 4.87e-25 105
MS.gene052196.t1 MTR_1g112270 34.228 149 90 3 111 259 56 196 1.24e-24 104
MS.gene052196.t1 MTR_1g112270 34.228 149 90 3 111 259 67 207 1.40e-24 104
MS.gene052196.t1 MTR_1g094115 34.899 149 89 3 111 259 59 199 2.81e-24 103
MS.gene052196.t1 MTR_1g016010 35.099 151 91 3 109 259 51 194 3.58e-23 99.8
MS.gene052196.t1 MTR_2g090745 35.570 149 88 4 111 259 19 159 2.64e-22 97.1
MS.gene052196.t1 MTR_2g099990 36.496 137 79 3 123 259 1 129 2.91e-22 96.7
MS.gene052196.t1 MTR_1g094115 35.878 131 76 3 129 259 60 182 6.22e-21 93.6
MS.gene052196.t1 MTR_1g093095 35.220 159 86 6 130 278 117 268 5.62e-20 88.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052196.t1 AT1G34370 58.788 165 62 2 113 271 19 183 1.05e-69 219
MS.gene052196.t1 AT1G34370 58.788 165 62 2 113 271 229 393 1.25e-68 223
MS.gene052196.t1 AT1G34370 58.788 165 62 2 113 271 229 393 1.25e-68 223
MS.gene052196.t1 AT5G22890 58.125 160 61 1 113 266 202 361 4.36e-66 212
MS.gene052196.t1 AT5G66730 37.821 156 89 3 104 259 37 184 1.77e-27 112
MS.gene052196.t1 AT5G44160 35.897 156 92 4 104 259 42 189 4.64e-27 110
MS.gene052196.t1 AT5G44160 36.424 151 88 4 109 259 8 150 7.30e-27 109
MS.gene052196.t1 AT1G03840 37.086 151 87 4 109 259 51 193 1.22e-26 110
MS.gene052196.t1 AT3G45260 35.762 151 90 3 109 259 49 192 4.06e-26 108
MS.gene052196.t1 AT3G45260 35.762 151 90 3 109 259 49 192 4.06e-26 108
MS.gene052196.t1 AT1G03840 36.913 149 86 4 111 259 51 191 4.33e-26 108
MS.gene052196.t1 AT5G60470 33.918 171 101 4 86 256 32 190 5.33e-26 107
MS.gene052196.t1 AT5G60470 33.918 171 101 4 86 256 36 194 5.57e-26 107
MS.gene052196.t1 AT5G03150 35.065 154 93 4 106 259 60 206 6.16e-26 108
MS.gene052196.t1 AT1G55110 37.086 151 88 4 109 259 73 216 1.66e-25 106
MS.gene052196.t1 AT1G55110 37.086 151 88 4 109 259 73 216 1.66e-25 106
MS.gene052196.t1 AT1G55110 37.086 151 88 4 109 259 73 216 1.66e-25 106
MS.gene052196.t1 AT5G60470 35.616 146 87 3 111 256 47 185 3.82e-25 105
MS.gene052196.t1 AT3G50700 35.897 156 92 3 104 259 39 186 1.35e-24 103
MS.gene052196.t1 AT2G02070 33.962 159 95 4 101 259 56 204 1.94e-24 104
MS.gene052196.t1 AT2G02070 33.962 159 95 4 101 259 56 204 1.94e-24 104
MS.gene052196.t1 AT3G13810 35.714 154 92 3 109 262 80 226 1.99e-24 103
MS.gene052196.t1 AT3G13810 35.714 154 92 3 109 262 80 226 1.99e-24 103
MS.gene052196.t1 AT1G14580 30.653 199 111 7 78 259 17 205 2.00e-24 103
MS.gene052196.t1 AT1G14580 30.653 199 111 7 78 259 17 205 2.00e-24 103
MS.gene052196.t1 AT3G13810 35.714 154 92 3 109 262 75 221 2.08e-24 103
MS.gene052196.t1 AT3G13810 35.714 154 92 3 109 262 61 207 2.09e-24 103
MS.gene052196.t1 AT1G14580 30.653 199 111 7 78 259 31 219 2.91e-24 103
MS.gene052196.t1 AT4G02670 36.424 151 89 3 109 259 65 208 5.85e-24 101
MS.gene052196.t1 AT4G02670 36.424 151 89 3 109 259 63 206 5.96e-24 101
MS.gene052196.t1 AT3G13810 35.526 152 91 3 111 262 69 213 1.31e-23 101
MS.gene052196.t1 AT3G13810 35.526 152 91 3 111 262 83 227 1.33e-23 101
MS.gene052196.t1 AT2G02080 33.557 149 91 3 111 259 66 206 1.51e-23 101
MS.gene052196.t1 AT2G02080 33.557 149 91 3 111 259 66 206 1.51e-23 101
MS.gene052196.t1 AT2G02080 33.557 149 91 3 111 259 66 206 1.51e-23 101
MS.gene052196.t1 AT2G02080 33.557 149 91 3 111 259 66 206 1.51e-23 101
MS.gene052196.t1 AT1G51220 39.161 143 79 7 130 268 180 318 1.80e-22 96.3
MS.gene052196.t1 AT3G20880 35.220 159 95 6 130 284 258 412 7.78e-22 95.5
MS.gene052196.t1 AT1G25250 36.913 149 87 4 109 256 20 162 1.62e-21 94.0
MS.gene052196.t1 AT1G25250 36.913 149 87 4 109 256 43 185 1.70e-21 94.4
MS.gene052196.t1 AT1G08290 35.000 160 93 6 130 282 182 337 3.46e-21 92.8
MS.gene052196.t1 AT1G34790 39.333 150 82 7 121 265 137 282 4.44e-21 92.0
MS.gene052196.t1 AT2G01940 36.842 152 82 6 109 256 54 195 6.61e-21 93.2
MS.gene052196.t1 AT1G68130 37.500 152 81 6 109 256 51 192 1.89e-20 91.7
MS.gene052196.t1 AT2G02080 32.847 137 84 3 123 259 1 129 3.64e-20 90.9
MS.gene052196.t1 AT2G02080 32.847 137 84 3 123 259 1 129 3.64e-20 90.9
MS.gene052196.t1 AT1G13290 32.308 195 123 6 81 270 55 245 8.45e-20 88.2
MS.gene052196.t1 AT3G57670 31.863 204 114 8 109 299 189 380 2.64e-19 88.2
MS.gene052196.t1 AT2G01940 37.143 140 74 6 109 244 54 183 3.18e-18 85.5

Find 98 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGAGTTCTTTCATGATTT+TGG 0.097161 3.2:-74396602 MS.gene052196:CDS
TGGGTAGTGTGATGGGAATC+TGG 0.233101 3.2:+74397487 None:intergenic
CTATTGCTTCGATGAAGTTT+TGG 0.237371 3.2:-74396567 MS.gene052196:CDS
GGACGACGACGCACGGTTAA+AGG 0.268870 3.2:+74397016 None:intergenic
TTTGCTGTGGATGAAGTATC+TGG 0.296757 3.2:+74397467 None:intergenic
TTGAGTAAAGATGATGAAAT+TGG 0.348705 3.2:-74396632 MS.gene052196:CDS
GAAGAATTATTCGATGCTTT+CGG 0.349953 3.2:-74396849 MS.gene052196:CDS
AACGCCGCCGCCCTCGTTCC+CGG 0.371943 3.2:-74397265 MS.gene052196:CDS
TCTAGGAAGGATAAGTTGTT+TGG 0.372634 3.2:-74396761 MS.gene052196:CDS
GAGGGCGGCGGCGTTGACTA+TGG 0.385405 3.2:+74397273 None:intergenic
GGTGGAGGACGGCAAAGATT+TGG 0.403975 3.2:+74397299 None:intergenic
GGACATGTTGCTTTGTTTGA+GGG 0.416752 3.2:-74396740 MS.gene052196:CDS
CGAAGCGGAACTTGAGGGTC+GGG 0.417024 3.2:+74397430 None:intergenic
AAAGGCTTCGCTAAAGCTTC+CGG 0.425013 3.2:+74397034 None:intergenic
CGGTTCTCGTGTCCGTTTGA+AGG 0.428660 3.2:-74396989 MS.gene052196:CDS
GGAATCTTCCACGGCGGTGA+GGG 0.432442 3.2:+74397195 None:intergenic
GAGCGTTGGTGGTGATGGAT+TGG 0.440346 3.2:+74397356 None:intergenic
TGCCGCATATCTCGCAGAAA+TGG 0.442862 3.2:+74397122 None:intergenic
TGGACATGTTGCTTTGTTTG+AGG 0.450086 3.2:-74396741 MS.gene052196:CDS
GAAATTCAAAGCATTGAAAT+CGG 0.454219 3.2:-74396942 MS.gene052196:CDS
TCCGCTTCGGAATCTATCCT+TGG 0.455820 3.2:-74397416 MS.gene052196:CDS
TCAAGCGCGACGCGAATCTC+CGG 0.460817 3.2:-74397093 MS.gene052196:CDS
CGGATTTGAAGAGTCATATG+AGG 0.460927 3.2:-74396829 MS.gene052196:CDS
GGCACCGGGAACGAGGGCGG+CGG 0.463287 3.2:+74397261 None:intergenic
GATTCCGAAGCGGAACTTGA+GGG 0.464978 3.2:+74397425 None:intergenic
AGGGAAGCAAGTGGTGGCTG+AGG 0.465601 3.2:-74396681 MS.gene052196:CDS
GGAGGAGAAGACGGAGGCAC+CGG 0.465619 3.2:+74397246 None:intergenic
AGCGTTGGTGGTGATGGATT+GGG 0.484580 3.2:+74397357 None:intergenic
CCTGCTGTTGTGTTGGATGA+TGG 0.487310 3.2:-74396710 MS.gene052196:CDS
GAATCTTCCACGGCGGTGAG+GGG 0.490348 3.2:+74397196 None:intergenic
GGGTAGTGTGATGGGAATCT+GGG 0.490735 3.2:+74397488 None:intergenic
AAGTATCTGGGTAGTGTGAT+GGG 0.491876 3.2:+74397480 None:intergenic
GAGCTCCACAATTTCGCAAT+CGG 0.498272 3.2:+74397174 None:intergenic
GACGGAGGCACCGGGAACGA+GGG 0.499392 3.2:+74397255 None:intergenic
CTAAGTGGAAGTGTTCTTGT+GGG 0.500097 3.2:-74396793 MS.gene052196:CDS
GACAGGAGCGTTGGTGGTGA+TGG 0.501841 3.2:+74397351 None:intergenic
CGTGTGCTCTCATGTGCATC+CGG 0.503672 3.2:+74397074 None:intergenic
TGGATTGGGACAGGAAGATT+CGG 0.505176 3.2:+74397371 None:intergenic
GTGTGATGGGAATCTGGGTT+CGG 0.507152 3.2:+74397493 None:intergenic
TGGTGGTGATGGATTGGGAC+AGG 0.519067 3.2:+74397362 None:intergenic
AATCTTCCACGGCGGTGAGG+GGG 0.520341 3.2:+74397197 None:intergenic
TTTCTGTTACATCCTTCAAA+CGG 0.521111 3.2:+74396977 None:intergenic
TTACTCAATGCCATCTCCAT+AGG 0.522738 3.2:+74396647 None:intergenic
AGATTCCGATTGCGAAATTG+TGG 0.528555 3.2:-74397179 MS.gene052196:CDS
TCATATGCCTGCTGTTGTGT+TGG 0.531256 3.2:-74396717 MS.gene052196:CDS
TGAGGAGAATGAAGATCCTA+TGG 0.534769 3.2:-74396663 MS.gene052196:CDS
TAAAGATGATGAAATTGGTA+TGG 0.536892 3.2:-74396627 MS.gene052196:CDS
AGATTACACGAATACATCTT+CGG 0.537959 3.2:+74396881 None:intergenic
TTCTGCGAGATATGCGGCAA+AGG 0.541688 3.2:-74397118 MS.gene052196:CDS
GTTAATTGATCATTGCTGAC+AGG 0.542229 3.2:+74397334 None:intergenic
GGAGGCACCGGGAACGAGGG+CGG 0.542744 3.2:+74397258 None:intergenic
GTTGTTGTTGACTGAATCAA+AGG 0.542842 3.2:+74397219 None:intergenic
GAGGAGAAGACGGAGGCACC+GGG 0.543680 3.2:+74397247 None:intergenic
CCGACCCTCAAGTTCCGCTT+CGG 0.543888 3.2:-74397429 MS.gene052196:CDS
CCATCATCCAACACAACAGC+AGG 0.544109 3.2:+74396710 None:intergenic
GAGGACGGCAAAGATTTGGT+TGG 0.549075 3.2:+74397303 None:intergenic
GTGTTGGATGATGGAGAGAA+AGG 0.549221 3.2:-74396701 MS.gene052196:CDS
AGACGGAGGCACCGGGAACG+AGG 0.549677 3.2:+74397254 None:intergenic
GTGTGAAGACTCATTTCAAG+AGG 0.550074 3.2:-74396913 MS.gene052196:CDS
GAAGCGGAACTTGAGGGTCG+GGG 0.550186 3.2:+74397431 None:intergenic
ATTGCTGACAGGAGCGTTGG+TGG 0.551289 3.2:+74397345 None:intergenic
CGGAATCTTCCACGGCGGTG+AGG 0.555882 3.2:+74397194 None:intergenic
AGATTCCGAAGCGGAACTTG+AGG 0.557075 3.2:+74397424 None:intergenic
GGCAAAGATTTGGTTGGAGA+CGG 0.557743 3.2:+74397309 None:intergenic
AAGCGGAACTTGAGGGTCGG+GGG 0.565688 3.2:+74397432 None:intergenic
GAAGTATCTGGGTAGTGTGA+TGG 0.565886 3.2:+74397479 None:intergenic
GAATGAAGATCCTATGGAGA+TGG 0.566144 3.2:-74396657 MS.gene052196:CDS
AATTGTGGAGCTCGACGCCA+TGG 0.568707 3.2:-74397164 MS.gene052196:CDS
ATCATTGCTGACAGGAGCGT+TGG 0.573157 3.2:+74397342 None:intergenic
AAAGGTCATGGAGGAGAAGA+CGG 0.577837 3.2:+74397237 None:intergenic
CGGATGCACATGAGAGCACA+CGG 0.577893 3.2:-74397073 MS.gene052196:CDS
TGTTGGATGATGGAGAGAAA+GGG 0.582040 3.2:-74396700 MS.gene052196:CDS
AACGGACACGAGAACCGTGT+TGG 0.584128 3.2:+74396995 None:intergenic
TTCGCAATCGGAATCTTCCA+CGG 0.585611 3.2:+74397186 None:intergenic
CGGCGGCGTTGACTATGGTA+TGG 0.588110 3.2:+74397278 None:intergenic
GGCAATGTGGTGAGTCTAAG+TGG 0.591829 3.2:-74396808 MS.gene052196:CDS
ATGTTCAGCTAGCAATTCCA+TGG 0.594022 3.2:+74397147 None:intergenic
TTGCTGTGGATGAAGTATCT+GGG 0.597399 3.2:+74397468 None:intergenic
CCGAAGCGGAACTTGAGGGT+CGG 0.601485 3.2:+74397429 None:intergenic
CCGTGCGTCGTCGTCCAACA+CGG 0.603358 3.2:-74397009 MS.gene052196:CDS
GACTATGGTATGGTGGAGGA+CGG 0.605416 3.2:+74397288 None:intergenic
CCGTGTTGGACGACGACGCA+CGG 0.605601 3.2:+74397009 None:intergenic
TCTAAGTGGAAGTGTTCTTG+TGG 0.613240 3.2:-74396794 MS.gene052196:CDS
CTCCATTTCTGCGAGATATG+CGG 0.627013 3.2:-74397124 MS.gene052196:CDS
AAAGATTTGGTTGGAGACGG+CGG 0.627067 3.2:+74397312 None:intergenic
GTTGACTGAATCAAAGGTCA+TGG 0.628225 3.2:+74397225 None:intergenic
CGGCGTTGACTATGGTATGG+TGG 0.628323 3.2:+74397281 None:intergenic
TGGAGAGAAAGGGAAGCAAG+TGG 0.632164 3.2:-74396690 MS.gene052196:CDS
CGTTGACTATGGTATGGTGG+AGG 0.634509 3.2:+74397284 None:intergenic
AGAGAAAGGGAAGCAAGTGG+TGG 0.675282 3.2:-74396687 MS.gene052196:CDS
ATCGATGCTAGTCTGAACCA+AGG 0.675325 3.2:+74397399 None:intergenic
CGGGAATCAGTTCAAGACGC+CGG 0.682583 3.2:-74397053 MS.gene052196:CDS
GACTGAATCAAAGGTCATGG+AGG 0.686847 3.2:+74397228 None:intergenic
GGATGCACATGAGAGCACAC+GGG 0.701290 3.2:-74397072 MS.gene052196:CDS
ACCAAGGATAGATTCCGAAG+CGG 0.716599 3.2:+74397415 None:intergenic
GCAATCGGAATCTTCCACGG+CGG 0.729614 3.2:+74397189 None:intergenic
AAGAGTCATATGAGGCAATG+TGG 0.740474 3.2:-74396821 MS.gene052196:CDS
GGTCATGGAGGAGAAGACGG+AGG 0.748482 3.2:+74397240 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TAAAGATGATGAAATTGGTA+TGG - chr3.2:74397425-74397444 MS.gene052196:CDS 25.0%
! TTGAGTAAAGATGATGAAAT+TGG - chr3.2:74397420-74397439 MS.gene052196:CDS 25.0%
!! AAGAGTTCTTTCATGATTTT+GGG - chr3.2:74397451-74397470 MS.gene052196:CDS 25.0%
!!! GAAATTCAAAGCATTGAAAT+CGG - chr3.2:74397110-74397129 MS.gene052196:CDS 25.0%
AGATTACACGAATACATCTT+CGG + chr3.2:74397174-74397193 None:intergenic 30.0%
GAAGAATTATTCGATGCTTT+CGG - chr3.2:74397203-74397222 MS.gene052196:CDS 30.0%
TTTCTGTTACATCCTTCAAA+CGG + chr3.2:74397078-74397097 None:intergenic 30.0%
! GAAGAGTTCTTTCATGATTT+TGG - chr3.2:74397450-74397469 MS.gene052196:CDS 30.0%
! TATTGCTTCGATGAAGTTTT+GGG - chr3.2:74397486-74397505 MS.gene052196:CDS 30.0%
GTTAATTGATCATTGCTGAC+AGG + chr3.2:74396721-74396740 None:intergenic 35.0%
TCTAGGAAGGATAAGTTGTT+TGG - chr3.2:74397291-74397310 MS.gene052196:CDS 35.0%
! CTATTGCTTCGATGAAGTTT+TGG - chr3.2:74397485-74397504 MS.gene052196:CDS 35.0%
! GTATTAGTTGGATTTTGCTG+TGG + chr3.2:74396601-74396620 None:intergenic 35.0%
! GTTGTTGTTGACTGAATCAA+AGG + chr3.2:74396836-74396855 None:intergenic 35.0%
AAGAGTCATATGAGGCAATG+TGG - chr3.2:74397231-74397250 MS.gene052196:CDS 40.0%
AAGTATCTGGGTAGTGTGAT+GGG + chr3.2:74396575-74396594 None:intergenic 40.0%
AGATTCCGATTGCGAAATTG+TGG - chr3.2:74396873-74396892 MS.gene052196:CDS 40.0%
ATGTTCAGCTAGCAATTCCA+TGG + chr3.2:74396908-74396927 None:intergenic 40.0%
CGGATTTGAAGAGTCATATG+AGG - chr3.2:74397223-74397242 MS.gene052196:CDS 40.0%
GAATGAAGATCCTATGGAGA+TGG - chr3.2:74397395-74397414 MS.gene052196:CDS 40.0%
GTGTGAAGACTCATTTCAAG+AGG - chr3.2:74397139-74397158 MS.gene052196:CDS 40.0%
GTTGACTGAATCAAAGGTCA+TGG + chr3.2:74396830-74396849 None:intergenic 40.0%
TGAGGAGAATGAAGATCCTA+TGG - chr3.2:74397389-74397408 MS.gene052196:CDS 40.0%
TTACTCAATGCCATCTCCAT+AGG + chr3.2:74397408-74397427 None:intergenic 40.0%
! TTGCTGTGGATGAAGTATCT+GGG + chr3.2:74396587-74396606 None:intergenic 40.0%
! TTTGCTGTGGATGAAGTATC+TGG + chr3.2:74396588-74396607 None:intergenic 40.0%
!! CTAAGTGGAAGTGTTCTTGT+GGG - chr3.2:74397259-74397278 MS.gene052196:CDS 40.0%
!! GGACATGTTGCTTTGTTTGA+GGG - chr3.2:74397312-74397331 MS.gene052196:CDS 40.0%
!! TCTAAGTGGAAGTGTTCTTG+TGG - chr3.2:74397258-74397277 MS.gene052196:CDS 40.0%
!! TGGACATGTTGCTTTGTTTG+AGG - chr3.2:74397311-74397330 MS.gene052196:CDS 40.0%
!! TGTTGGATGATGGAGAGAAA+GGG - chr3.2:74397352-74397371 MS.gene052196:CDS 40.0%
!!! CTTGTGGGAGTACTTTTTCT+AGG - chr3.2:74397274-74397293 MS.gene052196:CDS 40.0%
!!! TGGGAGTACTTTTTCTAGGA+AGG - chr3.2:74397278-74397297 MS.gene052196:CDS 40.0%
AAAGGCTTCGCTAAAGCTTC+CGG + chr3.2:74397021-74397040 None:intergenic 45.0%
AAAGGTCATGGAGGAGAAGA+CGG + chr3.2:74396818-74396837 None:intergenic 45.0%
ACCAAGGATAGATTCCGAAG+CGG + chr3.2:74396640-74396659 None:intergenic 45.0%
CTCCATTTCTGCGAGATATG+CGG - chr3.2:74396928-74396947 MS.gene052196:CDS 45.0%
GACTGAATCAAAGGTCATGG+AGG + chr3.2:74396827-74396846 None:intergenic 45.0%
GAGCTCCACAATTTCGCAAT+CGG + chr3.2:74396881-74396900 None:intergenic 45.0%
TCATATGCCTGCTGTTGTGT+TGG - chr3.2:74397335-74397354 MS.gene052196:CDS 45.0%
TTCGCAATCGGAATCTTCCA+CGG + chr3.2:74396869-74396888 None:intergenic 45.0%
! AAAGATTTGGTTGGAGACGG+CGG + chr3.2:74396743-74396762 None:intergenic 45.0%
! ATCGATGCTAGTCTGAACCA+AGG + chr3.2:74396656-74396675 None:intergenic 45.0%
! GAAGTATCTGGGTAGTGTGA+TGG + chr3.2:74396576-74396595 None:intergenic 45.0%
! GGCAAAGATTTGGTTGGAGA+CGG + chr3.2:74396746-74396765 None:intergenic 45.0%
! TGGATTGGGACAGGAAGATT+CGG + chr3.2:74396684-74396703 None:intergenic 45.0%
!! GTGTTGGATGATGGAGAGAA+AGG - chr3.2:74397351-74397370 MS.gene052196:CDS 45.0%
AGAGAAAGGGAAGCAAGTGG+TGG - chr3.2:74397365-74397384 MS.gene052196:CDS 50.0%
AGATTCCGAAGCGGAACTTG+AGG + chr3.2:74396631-74396650 None:intergenic 50.0%
CCATCATCCAACACAACAGC+AGG + chr3.2:74397345-74397364 None:intergenic 50.0%
CCTGCTGTTGTGTTGGATGA+TGG - chr3.2:74397342-74397361 MS.gene052196:CDS 50.0%
GATTCCGAAGCGGAACTTGA+GGG + chr3.2:74396630-74396649 None:intergenic 50.0%
GGCAATGTGGTGAGTCTAAG+TGG - chr3.2:74397244-74397263 MS.gene052196:CDS 50.0%
TCCGCTTCGGAATCTATCCT+TGG - chr3.2:74396636-74396655 MS.gene052196:CDS 50.0%
TGCCGCATATCTCGCAGAAA+TGG + chr3.2:74396933-74396952 None:intergenic 50.0%
TGGAGAGAAAGGGAAGCAAG+TGG - chr3.2:74397362-74397381 MS.gene052196:CDS 50.0%
TTCTGCGAGATATGCGGCAA+AGG - chr3.2:74396934-74396953 MS.gene052196:CDS 50.0%
! ATCATTGCTGACAGGAGCGT+TGG + chr3.2:74396713-74396732 None:intergenic 50.0%
! CGTTGACTATGGTATGGTGG+AGG + chr3.2:74396771-74396790 None:intergenic 50.0%
! GACTATGGTATGGTGGAGGA+CGG + chr3.2:74396767-74396786 None:intergenic 50.0%
! GAGGACGGCAAAGATTTGGT+TGG + chr3.2:74396752-74396771 None:intergenic 50.0%
! GGGTAGTGTGATGGGAATCT+GGG + chr3.2:74396567-74396586 None:intergenic 50.0%
! GTGTGATGGGAATCTGGGTT+CGG + chr3.2:74396562-74396581 None:intergenic 50.0%
! TGGGTAGTGTGATGGGAATC+TGG + chr3.2:74396568-74396587 None:intergenic 50.0%
!! AGCGTTGGTGGTGATGGATT+GGG + chr3.2:74396698-74396717 None:intergenic 50.0%
AACGGACACGAGAACCGTGT+TGG + chr3.2:74397060-74397079 None:intergenic 55.0%
AATTGTGGAGCTCGACGCCA+TGG - chr3.2:74396888-74396907 MS.gene052196:CDS 55.0%
CGGGAATCAGTTCAAGACGC+CGG - chr3.2:74396999-74397018 MS.gene052196:CDS 55.0%
CGGTTCTCGTGTCCGTTTGA+AGG - chr3.2:74397063-74397082 MS.gene052196:CDS 55.0%
CGTGTGCTCTCATGTGCATC+CGG + chr3.2:74396981-74397000 None:intergenic 55.0%
GCAATCGGAATCTTCCACGG+CGG + chr3.2:74396866-74396885 None:intergenic 55.0%
GGTGGAGGACGGCAAAGATT+TGG + chr3.2:74396756-74396775 None:intergenic 55.0%
! CGGATGCACATGAGAGCACA+CGG - chr3.2:74396979-74396998 MS.gene052196:CDS 55.0%
! GGATGCACATGAGAGCACAC+GGG - chr3.2:74396980-74396999 MS.gene052196:CDS 55.0%
!! ATTGCTGACAGGAGCGTTGG+TGG + chr3.2:74396710-74396729 None:intergenic 55.0%
!! CGGCGTTGACTATGGTATGG+TGG + chr3.2:74396774-74396793 None:intergenic 55.0%
!! GAGCGTTGGTGGTGATGGAT+TGG + chr3.2:74396699-74396718 None:intergenic 55.0%
!! TGAGGGTCGGGGGTATTAGT+TGG + chr3.2:74396613-74396632 None:intergenic 55.0%
!! TGGTGGTGATGGATTGGGAC+AGG + chr3.2:74396693-74396712 None:intergenic 55.0%
AAGCGGAACTTGAGGGTCGG+GGG + chr3.2:74396623-74396642 None:intergenic 60.0%
AATCTTCCACGGCGGTGAGG+GGG + chr3.2:74396858-74396877 None:intergenic 60.0%
AGGGAAGCAAGTGGTGGCTG+AGG - chr3.2:74397371-74397390 MS.gene052196:CDS 60.0%
CCGAAGCGGAACTTGAGGGT+CGG + chr3.2:74396626-74396645 None:intergenic 60.0%
CCGACCCTCAAGTTCCGCTT+CGG - chr3.2:74396623-74396642 MS.gene052196:CDS 60.0%
CGAAGCGGAACTTGAGGGTC+GGG + chr3.2:74396625-74396644 None:intergenic 60.0%
GAAGCGGAACTTGAGGGTCG+GGG + chr3.2:74396624-74396643 None:intergenic 60.0%
GAATCTTCCACGGCGGTGAG+GGG + chr3.2:74396859-74396878 None:intergenic 60.0%
GGAATCTTCCACGGCGGTGA+GGG + chr3.2:74396860-74396879 None:intergenic 60.0%
GGACGACGACGCACGGTTAA+AGG + chr3.2:74397039-74397058 None:intergenic 60.0%
GGTCATGGAGGAGAAGACGG+AGG + chr3.2:74396815-74396834 None:intergenic 60.0%
TCAAGCGCGACGCGAATCTC+CGG - chr3.2:74396959-74396978 MS.gene052196:CDS 60.0%
!! CGGCGGCGTTGACTATGGTA+TGG + chr3.2:74396777-74396796 None:intergenic 60.0%
!! GACAGGAGCGTTGGTGGTGA+TGG + chr3.2:74396704-74396723 None:intergenic 60.0%
CCGTGCGTCGTCGTCCAACA+CGG - chr3.2:74397043-74397062 MS.gene052196:CDS 65.0%
CCGTGTTGGACGACGACGCA+CGG + chr3.2:74397046-74397065 None:intergenic 65.0%
CGGAATCTTCCACGGCGGTG+AGG + chr3.2:74396861-74396880 None:intergenic 65.0%
! GGAGGAGAAGACGGAGGCAC+CGG + chr3.2:74396809-74396828 None:intergenic 65.0%
!! GAGGAGAAGACGGAGGCACC+GGG + chr3.2:74396808-74396827 None:intergenic 65.0%
CAACAACCCCCTCACCGCCG+TGG - chr3.2:74396849-74396868 MS.gene052196:CDS 70.0%
!! AGACGGAGGCACCGGGAACG+AGG + chr3.2:74396801-74396820 None:intergenic 70.0%
!! GACGGAGGCACCGGGAACGA+GGG + chr3.2:74396800-74396819 None:intergenic 70.0%
!! GAGGGCGGCGGCGTTGACTA+TGG + chr3.2:74396782-74396801 None:intergenic 70.0%
AACGCCGCCGCCCTCGTTCC+CGG - chr3.2:74396787-74396806 MS.gene052196:CDS 75.0%
!! GGAGGCACCGGGAACGAGGG+CGG + chr3.2:74396797-74396816 None:intergenic 75.0%
!! GGCACCGGGAACGAGGGCGG+CGG + chr3.2:74396794-74396813 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 74396550 74397524 74396550 ID=MS.gene052196
chr3.2 mRNA 74396550 74397524 74396550 ID=MS.gene052196.t1;Parent=MS.gene052196
chr3.2 exon 74396550 74397524 74396550 ID=MS.gene052196.t1.exon1;Parent=MS.gene052196.t1
chr3.2 CDS 74396550 74397524 74396550 ID=cds.MS.gene052196.t1;Parent=MS.gene052196.t1
Gene Sequence

>MS.gene052196

ATGTCTAATCCGAACCCAGATTCCCATCACACTACCCAGATACTTCATCCACAGCAAAATCCAACTAATACCCCCGACCCTCAAGTTCCGCTTCGGAATCTATCCTTGGTTCAGACTAGCATCGATTCTCTCCGAATCTTCCTGTCCCAATCCATCACCACCAACGCTCCTGTCAGCAATGATCAATTAACCGCCGTCTCCAACCAAATCTTTGCCGTCCTCCACCATACCATAGTCAACGCCGCCGCCCTCGTTCCCGGTGCCTCCGTCTTCTCCTCCATGACCTTTGATTCAGTCAACAACAACCCCCTCACCGCCGTGGAAGATTCCGATTGCGAAATTGTGGAGCTCGACGCCATGGAATTGCTAGCTGAACATCTCCATTTCTGCGAGATATGCGGCAAAGGCTTCAAGCGCGACGCGAATCTCCGGATGCACATGAGAGCACACGGGAATCAGTTCAAGACGCCGGAAGCTTTAGCGAAGCCTTTAACCGTGCGTCGTCGTCCAACACGGTTCTCGTGTCCGTTTGAAGGATGTAACAGAAACAAGAAGCATAAGAAATTCAAAGCATTGAAATCGGTGATTTGTGTGAAGACTCATTTCAAGAGGAGTCATTGTCCGAAGATGTATTCGTGTAATCTTTGTCGTAAGAAGAATTATTCGATGCTTTCGGATTTGAAGAGTCATATGAGGCAATGTGGTGAGTCTAAGTGGAAGTGTTCTTGTGGGAGTACTTTTTCTAGGAAGGATAAGTTGTTTGGACATGTTGCTTTGTTTGAGGGTCATATGCCTGCTGTTGTGTTGGATGATGGAGAGAAAGGGAAGCAAGTGGTGGCTGAGGAGAATGAAGATCCTATGGAGATGGCATTGAGTAAAGATGATGAAATTGGTATGGATGAAGAGTTCTTTCATGATTTTGGGTCTATTGATAACTATTGCTTCGATGAAGTTTTGGGATTTCGTCGTAGCTAG
Protein sequence

>MS.gene052196.t1

MSNPNPDSHHTTQILHPQQNPTNTPDPQVPLRNLSLVQTSIDSLRIFLSQSITTNAPVSNDQLTAVSNQIFAVLHHTIVNAAALVPGASVFSSMTFDSVNNNPLTAVEDSDCEIVELDAMELLAEHLHFCEICGKGFKRDANLRMHMRAHGNQFKTPEALAKPLTVRRRPTRFSCPFEGCNRNKKHKKFKALKSVICVKTHFKRSHCPKMYSCNLCRKKNYSMLSDLKSHMRQCGESKWKCSCGSTFSRKDKLFGHVALFEGHMPAVVLDDGEKGKQVVAEENEDPMEMALSKDDEIGMDEEFFHDFGSIDNYCFDEVLGFRRS