Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052343.t1 | AES63738.2 | 85.2 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 2.00E-55 | 224.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052343.t1 | G7INX6 | 85.2 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 1.4e-55 | 224.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene052343.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052343.t1 | MTR_2g012700 | 85.246 | 122 | 18 | 0 | 1 | 122 | 1 | 122 | 5.08e-75 | 218 |
| MS.gene052343.t1 | MTR_2g062330 | 81.667 | 120 | 21 | 1 | 3 | 122 | 23 | 141 | 3.51e-68 | 202 |
| MS.gene052343.t1 | MTR_7g117590 | 35.385 | 130 | 65 | 4 | 7 | 119 | 44 | 171 | 6.47e-21 | 82.8 |
| MS.gene052343.t1 | MTR_7g117585 | 36.634 | 101 | 62 | 1 | 18 | 116 | 42 | 142 | 4.90e-18 | 74.7 |
| MS.gene052343.t1 | MTR_0010s0310 | 41.758 | 91 | 51 | 1 | 22 | 110 | 150 | 240 | 1.28e-17 | 75.9 |
| MS.gene052343.t1 | MTR_3g008190 | 29.897 | 97 | 68 | 0 | 24 | 120 | 6 | 102 | 1.59e-16 | 69.7 |
| MS.gene052343.t1 | MTR_5g071410 | 34.653 | 101 | 64 | 1 | 18 | 116 | 165 | 265 | 3.58e-15 | 69.7 |
| MS.gene052343.t1 | MTR_6g477950 | 37.037 | 81 | 51 | 0 | 39 | 119 | 64 | 144 | 1.10e-13 | 65.9 |
| MS.gene052343.t1 | MTR_0010s0320 | 37.363 | 91 | 53 | 2 | 24 | 110 | 192 | 282 | 7.13e-13 | 63.5 |
| MS.gene052343.t1 | MTR_6g087720 | 33.721 | 86 | 56 | 1 | 24 | 108 | 214 | 299 | 1.10e-12 | 63.2 |
| MS.gene052343.t1 | MTR_0010s0290 | 37.363 | 91 | 53 | 2 | 24 | 110 | 223 | 313 | 1.37e-12 | 63.2 |
| MS.gene052343.t1 | MTR_5g078270 | 34.066 | 91 | 58 | 1 | 22 | 110 | 208 | 298 | 2.06e-12 | 62.4 |
| MS.gene052343.t1 | MTR_8g069780 | 25.843 | 89 | 66 | 0 | 31 | 119 | 67 | 155 | 8.62e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAATGTTACTGCTTCTATTT+TGG | 0.123735 | 4.4:-33576239 | MS.gene052343:CDS |
| AAAATTCCGATGTTCATTAT+TGG | 0.199581 | 4.4:-33576267 | MS.gene052343:CDS |
| GAAGTCAATGGTGCTGAATT+AGG | 0.200131 | 4.4:-33576298 | MS.gene052343:CDS |
| TACCAGTTTCTTCATCAAAA+AGG | 0.252196 | 4.4:+33576048 | None:intergenic |
| TGTATGCTACCGTCGTTAAC+TGG | 0.297739 | 4.4:-33575881 | MS.gene052343:CDS |
| CCTCGACGTGTTGCCGAGAA+TGG | 0.307864 | 4.4:-33576098 | MS.gene052343:CDS |
| CGGTCAATAATCCTCCTAAT+AGG | 0.355854 | 4.4:-33575905 | MS.gene052343:CDS |
| TTTGGATGGAAACAATGTTT+TGG | 0.356448 | 4.4:-33576221 | MS.gene052343:intron |
| AAGTCAATGGTGCTGAATTA+GGG | 0.416476 | 4.4:-33576297 | MS.gene052343:CDS |
| ACGGTAGCATACATCCTATT+AGG | 0.440754 | 4.4:+33575891 | None:intergenic |
| ATTGGTATACAAACCATTCT+CGG | 0.470714 | 4.4:+33576085 | None:intergenic |
| GTATGCTACCGTCGTTAACT+GGG | 0.522932 | 4.4:-33575880 | MS.gene052343:CDS |
| GCATGGATCAGAAATACATA+GGG | 0.559318 | 4.4:-33575989 | MS.gene052343:CDS |
| AGCATGGATCAGAAATACAT+AGG | 0.564126 | 4.4:-33575990 | MS.gene052343:CDS |
| CTTGAAAAGTGCCTAAAAGT+TGG | 0.570138 | 4.4:-33575945 | MS.gene052343:CDS |
| AGAAATACATAGGGAGAGGA+TGG | 0.573439 | 4.4:-33575980 | MS.gene052343:CDS |
| GATCAGAAATACATAGGGAG+AGG | 0.587262 | 4.4:-33575984 | MS.gene052343:CDS |
| TCACACTGCGTCAAGAAGCA+TGG | 0.587686 | 4.4:-33576006 | MS.gene052343:CDS |
| CCATTCTCGGCAACACGTCG+AGG | 0.599354 | 4.4:+33576098 | None:intergenic |
| GAGATTGGAGTTGAAGTCAA+TGG | 0.619642 | 4.4:-33576310 | MS.gene052343:CDS |
| GTAGCATACATCCTATTAGG+AGG | 0.633251 | 4.4:+33575894 | None:intergenic |
| ATACTTCCAATAATGAACAT+CGG | 0.646998 | 4.4:+33576261 | None:intergenic |
| TAATCAGTCCCAGTTAACGA+CGG | 0.733495 | 4.4:+33575872 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCCGATGTTCATTAT+TGG | - | chr4.4:33575924-33575943 | MS.gene052343:CDS | 25.0% |
| ! | ATACTTCCAATAATGAACAT+CGG | + | chr4.4:33575933-33575952 | None:intergenic | 25.0% |
| !! | AAGGAGAATTTTAGATTGAT+TGG | + | chr4.4:33576127-33576146 | None:intergenic | 25.0% |
| !! | TAATGTTACTGCTTCTATTT+TGG | - | chr4.4:33575952-33575971 | MS.gene052343:CDS | 25.0% |
| ATTGGTATACAAACCATTCT+CGG | + | chr4.4:33576109-33576128 | None:intergenic | 30.0% | |
| TACCAGTTTCTTCATCAAAA+AGG | + | chr4.4:33576146-33576165 | None:intergenic | 30.0% | |
| ! | AAACAACATCACCAACTTTT+AGG | + | chr4.4:33576260-33576279 | None:intergenic | 30.0% |
| ! | TTTGGATGGAAACAATGTTT+TGG | - | chr4.4:33575970-33575989 | MS.gene052343:CDS | 30.0% |
| AGCATGGATCAGAAATACAT+AGG | - | chr4.4:33576201-33576220 | MS.gene052343:intron | 35.0% | |
| GCATGGATCAGAAATACATA+GGG | - | chr4.4:33576202-33576221 | MS.gene052343:intron | 35.0% | |
| ! | AAGTCAATGGTGCTGAATTA+GGG | - | chr4.4:33575894-33575913 | MS.gene052343:CDS | 35.0% |
| ! | CTCCTTTTTGATGAAGAAAC+TGG | - | chr4.4:33576141-33576160 | MS.gene052343:intron | 35.0% |
| !! | CTTGAAAAGTGCCTAAAAGT+TGG | - | chr4.4:33576246-33576265 | MS.gene052343:CDS | 35.0% |
| !! | GTTACTGCTTCTATTTTGGA+TGG | - | chr4.4:33575956-33575975 | MS.gene052343:CDS | 35.0% |
| !!! | GGAAACAATGTTTTGGTTAG+TGG | - | chr4.4:33575977-33575996 | MS.gene052343:CDS | 35.0% |
| !!! | TGGTGATGTTGTTTTGTTCA+CGG | - | chr4.4:33576266-33576285 | MS.gene052343:CDS | 35.0% |
| ACGGTAGCATACATCCTATT+AGG | + | chr4.4:33576303-33576322 | None:intergenic | 40.0% | |
| AGAAATACATAGGGAGAGGA+TGG | - | chr4.4:33576211-33576230 | MS.gene052343:intron | 40.0% | |
| CGGTCAATAATCCTCCTAAT+AGG | - | chr4.4:33576286-33576305 | MS.gene052343:CDS | 40.0% | |
| GAGATTGGAGTTGAAGTCAA+TGG | - | chr4.4:33575881-33575900 | MS.gene052343:CDS | 40.0% | |
| GATCAGAAATACATAGGGAG+AGG | - | chr4.4:33576207-33576226 | MS.gene052343:intron | 40.0% | |
| GTAGCATACATCCTATTAGG+AGG | + | chr4.4:33576300-33576319 | None:intergenic | 40.0% | |
| ! | GAAGTCAATGGTGCTGAATT+AGG | - | chr4.4:33575893-33575912 | MS.gene052343:CDS | 40.0% |
| GTATGCTACCGTCGTTAACT+GGG | - | chr4.4:33576311-33576330 | MS.gene052343:CDS | 45.0% | |
| TGTATGCTACCGTCGTTAAC+TGG | - | chr4.4:33576310-33576329 | MS.gene052343:CDS | 45.0% | |
| TCACACTGCGTCAAGAAGCA+TGG | - | chr4.4:33576185-33576204 | MS.gene052343:intron | 50.0% | |
| CCATTCTCGGCAACACGTCG+AGG | + | chr4.4:33576096-33576115 | None:intergenic | 60.0% | |
| CCTCGACGTGTTGCCGAGAA+TGG | - | chr4.4:33576093-33576112 | MS.gene052343:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 33575875 | 33576338 | 33575875 | ID=MS.gene052343 |
| chr4.4 | mRNA | 33575875 | 33576338 | 33575875 | ID=MS.gene052343.t1;Parent=MS.gene052343 |
| chr4.4 | exon | 33576222 | 33576338 | 33576222 | ID=MS.gene052343.t1.exon1;Parent=MS.gene052343.t1 |
| chr4.4 | CDS | 33576222 | 33576338 | 33576222 | ID=cds.MS.gene052343.t1;Parent=MS.gene052343.t1 |
| chr4.4 | exon | 33575875 | 33576126 | 33575875 | ID=MS.gene052343.t1.exon2;Parent=MS.gene052343.t1 |
| chr4.4 | CDS | 33575875 | 33576126 | 33575875 | ID=cds.MS.gene052343.t1;Parent=MS.gene052343.t1 |
| Gene Sequence |
| Protein sequence |
