AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052431

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052431.t1 MTR_5g078270 38.305 295 169 5 19 302 17 309 4.59e-61 196
MS.gene052431.t1 MTR_0010s0290 34.576 295 173 6 19 302 39 324 5.09e-52 174
MS.gene052431.t1 MTR_0010s0320 33.115 305 180 7 9 302 2 293 1.74e-48 164
MS.gene052431.t1 MTR_1g054480 33.916 286 176 5 22 303 19 295 1.77e-44 153
MS.gene052431.t1 MTR_0010s0310 34.191 272 126 6 22 291 20 240 1.21e-34 126
MS.gene052431.t1 MTR_1g050480 31.276 243 122 5 12 253 8 206 1.31e-23 99.0
MS.gene052431.t1 MTR_2g012700 30.189 106 73 1 196 300 14 119 6.15e-13 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 94 sgRNAs with CRISPR-Local

Find 127 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACGACGTACCATATTT+TGG 0.218433 6.1:+74976069 MS.gene052431:CDS
CATCTGATAATTGTAGAAAT+TGG 0.239747 6.1:-74976263 None:intergenic
CCGAGGTATGTTGATGTTAT+TGG 0.249280 6.1:+74976153 MS.gene052431:CDS
TTTAAGATCCTTACTTGTTC+AGG 0.287517 6.1:-74975700 None:intergenic
AGTCTTCAATCTTAGACTTT+TGG 0.299655 6.1:-74976660 None:intergenic
TATATGGTTGACTACAGATT+TGG 0.309041 6.1:-74976510 None:intergenic
TTCAATATGCGAATTGTTGA+TGG 0.313877 6.1:+74976042 MS.gene052431:CDS
TCCAATGGAGTTGACCCTTC+AGG 0.316164 6.1:+74975646 MS.gene052431:CDS
TTCCAATCGACGACAAATTC+AGG 0.317981 6.1:-74975838 None:intergenic
CCTTTGAGTTTGCAATGTTA+TGG 0.330298 6.1:+74976202 MS.gene052431:CDS
GGATAATGGAATCCAAAATA+TGG 0.331913 6.1:-74976081 None:intergenic
ATTTCCAGCCGGATCTTTGA+TGG 0.339598 6.1:-74975890 None:intergenic
ATATGGTTGACTACAGATTT+GGG 0.342902 6.1:-74976509 None:intergenic
CTCCAATGGAACTCAATGTT+AGG 0.348995 6.1:+74976714 MS.gene052431:CDS
CTCGGATTGACTTGAAGGTT+TGG 0.372952 6.1:-74976135 None:intergenic
GGCCGACCAGAACATAGTTT+TGG 0.383031 6.1:+74976541 MS.gene052431:CDS
CATACCTCGGATTGACTTGA+AGG 0.392440 6.1:-74976140 None:intergenic
GGTCGGCCACAAAAGGTATA+TGG 0.404072 6.1:-74976526 None:intergenic
GGGAGGAAAAGACCACCGTC+AGG 0.427903 6.1:-74976180 None:intergenic
CAACAATTCGCATATTGAAC+TGG 0.435687 6.1:-74976038 None:intergenic
TCACTTCGGCCCTTAGCAAC+TGG 0.436275 6.1:-74976360 None:intergenic
CTCCTGAATTTGTCGTCGAT+TGG 0.443810 6.1:+74975836 MS.gene052431:CDS
CATAACATTGCAAACTCAAA+GGG 0.444223 6.1:-74976201 None:intergenic
CCAATGGAGTTGACCCTTCA+GGG 0.452496 6.1:+74975647 MS.gene052431:CDS
CACAGTACCTTGCAATAATC+CGG 0.462888 6.1:-74975966 None:intergenic
TCCCTACTCCACTTTCCAAC+GGG 0.468155 6.1:+74975233 MS.gene052431:CDS
TGTAACCAGGCCAGTTGCTA+AGG 0.483856 6.1:+74976350 MS.gene052431:CDS
TGCTAAGGGCCGAAGTGACT+TGG 0.484950 6.1:+74976365 MS.gene052431:CDS
ACCCTAACATTGAGTTCCAT+TGG 0.485090 6.1:-74976716 None:intergenic
CATCAACCAAAACTATGTTC+TGG 0.486969 6.1:-74976547 None:intergenic
CAATTTACATAGGGAGTGGC+TGG 0.490021 6.1:+74976627 MS.gene052431:CDS
ATGTTCTGGTCGGCCACAAA+AGG 0.492846 6.1:-74976533 None:intergenic
ATGTTAGGGTTCTCCGTCGC+AGG 0.496923 6.1:+74976729 MS.gene052431:CDS
CCCTGAAGGGTCAACTCCAT+TGG 0.500515 6.1:-74975647 None:intergenic
TCCAATGGAACTCAATGTTA+GGG 0.501785 6.1:+74976715 MS.gene052431:CDS
TGTCGGATCCATCAAAGATC+CGG 0.506141 6.1:+74975882 MS.gene052431:CDS
ATAATTGTAGAAATTGGATA+AGG 0.509582 6.1:-74976257 None:intergenic
ATGCGAATTGTTGATGGAGA+AGG 0.509644 6.1:+74976048 MS.gene052431:CDS
TTGACTTACCCGTTGGAAAG+TGG 0.513614 6.1:-74975241 None:intergenic
ATTCAGGAGGGATTTCAATA+CGG 0.516187 6.1:-74975822 None:intergenic
TTATGGATGGAAGAACTTAA+CGG 0.519426 6.1:+74976219 MS.gene052431:CDS
AAAGTGGTTGGTGCCCCTGA+AGG 0.522492 6.1:-74975661 None:intergenic
CCATAACATTGCAAACTCAA+AGG 0.523655 6.1:-74976202 None:intergenic
TCGGCCCTTAGCAACTGGCC+TGG 0.525594 6.1:-74976355 None:intergenic
TCGGAAGAAGCAATTTACAT+AGG 0.532988 6.1:+74976617 MS.gene052431:CDS
TGAATTTGTCGTCGATTGGA+AGG 0.536815 6.1:+74975840 MS.gene052431:CDS
GAGGAAAAGACCACCGTCAG+GGG 0.542442 6.1:-74976178 None:intergenic
CGGAAGAAGCAATTTACATA+GGG 0.544470 6.1:+74976618 MS.gene052431:CDS
GTCCACTGAAAGTCAAAAGC+AGG 0.551689 6.1:-74976321 None:intergenic
GGAGGAAAAGACCACCGTCA+GGG 0.553757 6.1:-74976179 None:intergenic
GTATCCATCGATACTTTGCA+TGG 0.555610 6.1:-74976590 None:intergenic
CGGATTATTGCAAGGTACTG+TGG 0.558040 6.1:+74975967 MS.gene052431:CDS
TAGTCAAGAATTACCTTCTG+CGG 0.559691 6.1:-74976000 None:intergenic
TGCGAATTGTTGATGGAGAA+GGG 0.561259 6.1:+74976049 MS.gene052431:CDS
GTCCCTACTCCACTTTCCAA+CGG 0.564413 6.1:+74975232 MS.gene052431:CDS
GAAGGTAATTCTTGACTACA+AGG 0.567645 6.1:+74976005 MS.gene052431:CDS
AACTGGCCTGGTTACATCCA+AGG 0.574011 6.1:-74976343 None:intergenic
ATATACCATGGTATCAAAAG+TGG 0.579646 6.1:-74975677 None:intergenic
GGATCCATCAAAGATCCGGC+TGG 0.581763 6.1:+74975886 MS.gene052431:CDS
AAGTGGTTGGTGCCCCTGAA+GGG 0.581816 6.1:-74975660 None:intergenic
CTAACATTGAGTTCCATTGG+AGG 0.582212 6.1:-74976713 None:intergenic
AAAAGTCTAAGATTGAAGAC+TGG 0.584520 6.1:+74976662 MS.gene052431:CDS
ACATGTTCATCAGAGGCGGC+CGG 0.584533 6.1:+74975947 MS.gene052431:CDS
TTGTCTGACATGTTCATCAG+AGG 0.585532 6.1:+74975940 MS.gene052431:CDS
AGGAAAAGACCACCGTCAGG+GGG 0.587565 6.1:-74976177 None:intergenic
AGATGAACATAATGAGACTT+CGG 0.590070 6.1:+74976281 MS.gene052431:CDS
TGGACTGGACAATCCTCCAA+TGG 0.590635 6.1:+74976700 MS.gene052431:CDS
GAAGCAATTTACATAGGGAG+TGG 0.592040 6.1:+74976623 MS.gene052431:CDS
TCTGACATGTTCATCAGAGG+CGG 0.593190 6.1:+74975943 MS.gene052431:CDS
ACCCGTTGGAAAGTGGAGTA+GGG 0.595752 6.1:-74975234 None:intergenic
TGGTTTCAATGAACCGCAGA+AGG 0.596257 6.1:+74975987 MS.gene052431:CDS
AGTGGACCTTGGATGTAACC+AGG 0.598459 6.1:+74976337 MS.gene052431:CDS
ATTCCCATGCAAAGTATCGA+TGG 0.599282 6.1:+74976586 MS.gene052431:CDS
TGAGTTTGCAATGTTATGGA+TGG 0.599799 6.1:+74976206 MS.gene052431:CDS
AGCATCGCTGTACAAAATGT+CGG 0.608796 6.1:+74975865 MS.gene052431:CDS
TATCCATCGATACTTTGCAT+GGG 0.609143 6.1:-74976589 None:intergenic
ACCATGGTATCAAAAGTGGT+TGG 0.609782 6.1:-74975673 None:intergenic
TACCCGTTGGAAAGTGGAGT+AGG 0.614917 6.1:-74975235 None:intergenic
AACCAAAACTATGTTCTGGT+CGG 0.619760 6.1:-74976543 None:intergenic
GTGTACTAACCAAGTCACTT+CGG 0.625729 6.1:-74976374 None:intergenic
CCAATAACATCAACATACCT+CGG 0.630235 6.1:-74976153 None:intergenic
GTAACCAGGCCAGTTGCTAA+GGG 0.632798 6.1:+74976351 MS.gene052431:CDS
TGCTTCAGTGAGACCTGCGA+CGG 0.645760 6.1:-74976742 None:intergenic
GAGGCGGCCGGATTATTGCA+AGG 0.655116 6.1:+74975959 MS.gene052431:CDS
CAATCGACGACAAATTCAGG+AGG 0.655933 6.1:-74975835 None:intergenic
CAATGGAGTTGACCCTTCAG+GGG 0.660593 6.1:+74975648 MS.gene052431:CDS
CAAACCTTCAAGTCAATCCG+AGG 0.662887 6.1:+74976136 MS.gene052431:CDS
TATGGTTGACTACAGATTTG+GGG 0.664603 6.1:-74976508 None:intergenic
TACTTGTTCAGGATATACCA+TGG 0.667959 6.1:-74975689 None:intergenic
AATCGACGACAAATTCAGGA+GGG 0.676312 6.1:-74975834 None:intergenic
ACATTGCAAACTCAAAGGGG+AGG 0.676366 6.1:-74976197 None:intergenic
GGAAAGTGGAGTAGGGACCA+TGG 0.684071 6.1:-74975227 None:intergenic
AGTATCGATGGATACAACAT+CGG 0.685684 6.1:+74976598 MS.gene052431:CDS
ATAACATTGCAAACTCAAAG+GGG 0.714085 6.1:-74976200 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATATATTTTGATGTA+GGG - chr6.1:74975288-74975307 None:intergenic 10.0%
!! ATCATTAGAAAATTCAAAAA+AGG - chr6.1:74975344-74975363 None:intergenic 15.0%
!! TTCAAAAAAGGAAATTATAT+TGG - chr6.1:74975332-74975351 None:intergenic 15.0%
!!! CAAAAAATATATTTTGATGT+AGG - chr6.1:74975289-74975308 None:intergenic 15.0%
!!! CTACATCAAAATATATTTTT+TGG + chr6.1:74975287-74975306 MS.gene052431:intron 15.0%
!! ATAATTGTAGAAATTGGATA+AGG - chr6.1:74976260-74976279 None:intergenic 20.0%
!! ATATGTTTGTTTAAATATGC+AGG - chr6.1:74976471-74976490 None:intergenic 20.0%
!! TAGCAAAATAATGATTAGAT+GGG - chr6.1:74975445-74975464 None:intergenic 20.0%
!!! ATTTTTTTTAGACAATCCAA+TGG + chr6.1:74975631-74975650 MS.gene052431:intron 20.0%
!!! CATTTGTTTTCATATATACT+AGG + chr6.1:74975380-74975399 MS.gene052431:intron 20.0%
! CATCTGATAATTGTAGAAAT+TGG - chr6.1:74976266-74976285 None:intergenic 25.0%
! GAAAACAAATGTAAAGAATG+TGG - chr6.1:74975371-74975390 None:intergenic 25.0%
! GTAGCAAAATAATGATTAGA+TGG - chr6.1:74975446-74975465 None:intergenic 25.0%
!! ATCTTAGCATGAATATTTTC+AGG - chr6.1:74976105-74976124 None:intergenic 25.0%
!! TTGTTTTCATATATACTAGG+AGG + chr6.1:74975383-74975402 MS.gene052431:intron 25.0%
!!! CATGAATATTTTCAGGATAA+TGG - chr6.1:74976098-74976117 None:intergenic 25.0%
AGATGAACATAATGAGACTT+CGG + chr6.1:74976281-74976300 MS.gene052431:CDS 30.0%
AGTGATTTATGTTCCATATG+TGG + chr6.1:74975532-74975551 MS.gene052431:intron 30.0%
ATAACATTGCAAACTCAAAG+GGG - chr6.1:74976203-74976222 None:intergenic 30.0%
ATATACCATGGTATCAAAAG+TGG - chr6.1:74975680-74975699 None:intergenic 30.0%
ATATGGTTGACTACAGATTT+GGG - chr6.1:74976512-74976531 None:intergenic 30.0%
CATAACATTGCAAACTCAAA+GGG - chr6.1:74976204-74976223 None:intergenic 30.0%
GTGATTTATGTTCCATATGT+GGG + chr6.1:74975533-74975552 MS.gene052431:intron 30.0%
TATATGGTTGACTACAGATT+TGG - chr6.1:74976513-74976532 None:intergenic 30.0%
TTATGGATGGAAGAACTTAA+CGG + chr6.1:74976219-74976238 MS.gene052431:CDS 30.0%
TTCAATATGCGAATTGTTGA+TGG + chr6.1:74976042-74976061 MS.gene052431:CDS 30.0%
! AAAAGTCTAAGATTGAAGAC+TGG + chr6.1:74976662-74976681 MS.gene052431:CDS 30.0%
! AGTCTTCAATCTTAGACTTT+TGG - chr6.1:74976663-74976682 None:intergenic 30.0%
! CAACACCATATCTAATCTTT+TGG + chr6.1:74975261-74975280 MS.gene052431:intron 30.0%
! TAAGTAGTATAGTTGATGCT+TGG + chr6.1:74975558-74975577 MS.gene052431:intron 30.0%
! TTTAAGATCCTTACTTGTTC+AGG - chr6.1:74975703-74975722 None:intergenic 30.0%
!! GGATAATGGAATCCAAAATA+TGG - chr6.1:74976084-74976103 None:intergenic 30.0%
AACCAAAACTATGTTCTGGT+CGG - chr6.1:74976546-74976565 None:intergenic 35.0%
AGGGACCAAAAGATTAGATA+TGG - chr6.1:74975269-74975288 None:intergenic 35.0%
ATACTACTTACACCCACATA+TGG - chr6.1:74975548-74975567 None:intergenic 35.0%
ATCCCAAAAAAATTTCCAGC+CGG - chr6.1:74975904-74975923 None:intergenic 35.0%
ATTCAGGAGGGATTTCAATA+CGG - chr6.1:74975825-74975844 None:intergenic 35.0%
CAACAATTCGCATATTGAAC+TGG - chr6.1:74976041-74976060 None:intergenic 35.0%
CATCAACCAAAACTATGTTC+TGG - chr6.1:74976550-74976569 None:intergenic 35.0%
CCAATAACATCAACATACCT+CGG - chr6.1:74976156-74976175 None:intergenic 35.0%
CCATAACATTGCAAACTCAA+AGG - chr6.1:74976205-74976224 None:intergenic 35.0%
CGGAAGAAGCAATTTACATA+GGG + chr6.1:74976618-74976637 MS.gene052431:CDS 35.0%
GAAGGTAATTCTTGACTACA+AGG + chr6.1:74976005-74976024 MS.gene052431:CDS 35.0%
TACTTGTTCAGGATATACCA+TGG - chr6.1:74975692-74975711 None:intergenic 35.0%
TAGTCAAGAATTACCTTCTG+CGG - chr6.1:74976003-74976022 None:intergenic 35.0%
TATCCATCGATACTTTGCAT+GGG - chr6.1:74976592-74976611 None:intergenic 35.0%
TATGGTTGACTACAGATTTG+GGG - chr6.1:74976511-74976530 None:intergenic 35.0%
TCCAATGGAACTCAATGTTA+GGG + chr6.1:74976715-74976734 MS.gene052431:CDS 35.0%
TCGGAAGAAGCAATTTACAT+AGG + chr6.1:74976617-74976636 MS.gene052431:CDS 35.0%
TGAGTTTGCAATGTTATGGA+TGG + chr6.1:74976206-74976225 MS.gene052431:CDS 35.0%
TGGTATATCCTGAACAAGTA+AGG + chr6.1:74975692-74975711 MS.gene052431:intron 35.0%
! AGTCAACCATATACCTTTTG+TGG + chr6.1:74976520-74976539 MS.gene052431:CDS 35.0%
! CCTTTGAGTTTGCAATGTTA+TGG + chr6.1:74976202-74976221 MS.gene052431:CDS 35.0%
!! AGTATCGATGGATACAACAT+CGG + chr6.1:74976598-74976617 MS.gene052431:CDS 35.0%
!!! ATCCGGCTGGAAATTTTTTT+GGG + chr6.1:74975899-74975918 MS.gene052431:CDS 35.0%
AATCGACGACAAATTCAGGA+GGG - chr6.1:74975837-74975856 None:intergenic 40.0%
ACCATGGTATCAAAAGTGGT+TGG - chr6.1:74975676-74975695 None:intergenic 40.0%
ACCCTAACATTGAGTTCCAT+TGG - chr6.1:74976719-74976738 None:intergenic 40.0%
AGCATCGCTGTACAAAATGT+CGG + chr6.1:74975865-74975884 MS.gene052431:CDS 40.0%
CACAGTACCTTGCAATAATC+CGG - chr6.1:74975969-74975988 None:intergenic 40.0%
CCGAGGTATGTTGATGTTAT+TGG + chr6.1:74976153-74976172 MS.gene052431:CDS 40.0%
CTAACATTGAGTTCCATTGG+AGG - chr6.1:74976716-74976735 None:intergenic 40.0%
CTCCAATGGAACTCAATGTT+AGG + chr6.1:74976714-74976733 MS.gene052431:CDS 40.0%
GAAGCAATTTACATAGGGAG+TGG + chr6.1:74976623-74976642 MS.gene052431:CDS 40.0%
GTATCCATCGATACTTTGCA+TGG - chr6.1:74976593-74976612 None:intergenic 40.0%
GTGTACTAACCAAGTCACTT+CGG - chr6.1:74976377-74976396 None:intergenic 40.0%
TTCCAATCGACGACAAATTC+AGG - chr6.1:74975841-74975860 None:intergenic 40.0%
TTGTCTGACATGTTCATCAG+AGG + chr6.1:74975940-74975959 MS.gene052431:CDS 40.0%
! ACTGGTGACATGCTTTTCTT+TGG + chr6.1:74976680-74976699 MS.gene052431:CDS 40.0%
! ATGCGAATTGTTGATGGAGA+AGG + chr6.1:74976048-74976067 MS.gene052431:CDS 40.0%
! TGAATTTGTCGTCGATTGGA+AGG + chr6.1:74975840-74975859 MS.gene052431:CDS 40.0%
! TGACATGCTTTTCTTTGGAC+TGG + chr6.1:74976685-74976704 MS.gene052431:CDS 40.0%
! TGCGAATTGTTGATGGAGAA+GGG + chr6.1:74976049-74976068 MS.gene052431:CDS 40.0%
!! ACCAACCACTTTTGATACCA+TGG + chr6.1:74975672-74975691 MS.gene052431:CDS 40.0%
!! ATTCCCATGCAAAGTATCGA+TGG + chr6.1:74976586-74976605 MS.gene052431:CDS 40.0%
!!! GATCCGGCTGGAAATTTTTT+TGG + chr6.1:74975898-74975917 MS.gene052431:CDS 40.0%
!!! TTCCTGCTTTTGACTTTCAG+TGG + chr6.1:74976319-74976338 MS.gene052431:CDS 40.0%
!!! TTTTGACTTTCAGTGGACCT+TGG + chr6.1:74976326-74976345 MS.gene052431:CDS 40.0%
ACATTGCAAACTCAAAGGGG+AGG - chr6.1:74976200-74976219 None:intergenic 45.0%
ATTTCCAGCCGGATCTTTGA+TGG - chr6.1:74975893-74975912 None:intergenic 45.0%
CAAACCTTCAAGTCAATCCG+AGG + chr6.1:74976136-74976155 MS.gene052431:CDS 45.0%
CAATCGACGACAAATTCAGG+AGG - chr6.1:74975838-74975857 None:intergenic 45.0%
CAATTTACATAGGGAGTGGC+TGG + chr6.1:74976627-74976646 MS.gene052431:CDS 45.0%
CTCCTGAATTTGTCGTCGAT+TGG + chr6.1:74975836-74975855 MS.gene052431:CDS 45.0%
GTCCACTGAAAGTCAAAAGC+AGG - chr6.1:74976324-74976343 None:intergenic 45.0%
TCTGACATGTTCATCAGAGG+CGG + chr6.1:74975943-74975962 MS.gene052431:CDS 45.0%
TGGTTTCAATGAACCGCAGA+AGG + chr6.1:74975987-74976006 MS.gene052431:CDS 45.0%
TGTCGGATCCATCAAAGATC+CGG + chr6.1:74975882-74975901 MS.gene052431:CDS 45.0%
TTGACTTACCCGTTGGAAAG+TGG - chr6.1:74975244-74975263 None:intergenic 45.0%
! GGGAACGACGTACCATATTT+TGG + chr6.1:74976069-74976088 MS.gene052431:CDS 45.0%
!! CATACCTCGGATTGACTTGA+AGG - chr6.1:74976143-74976162 None:intergenic 45.0%
!! CGGATTATTGCAAGGTACTG+TGG + chr6.1:74975967-74975986 MS.gene052431:CDS 45.0%
!! CTCGGATTGACTTGAAGGTT+TGG - chr6.1:74976138-74976157 None:intergenic 45.0%
!! TATGGTGTTGACTTACCCGT+TGG - chr6.1:74975251-74975270 None:intergenic 45.0%
!! TCTTATATAAATTATAATAT+AGG + chr6.1:74975798-74975817 MS.gene052431:intron 5.0%
!!! ATATAAAAAATAGATTTTAA+TGG - chr6.1:74976401-74976420 None:intergenic 5.0%
ACCCGTTGGAAAGTGGAGTA+GGG - chr6.1:74975237-74975256 None:intergenic 50.0%
AGTGGACCTTGGATGTAACC+AGG + chr6.1:74976337-74976356 MS.gene052431:CDS 50.0%
ATGTTCTGGTCGGCCACAAA+AGG - chr6.1:74976536-74976555 None:intergenic 50.0%
CAATGGAGTTGACCCTTCAG+GGG + chr6.1:74975648-74975667 MS.gene052431:CDS 50.0%
CCAATGGAGTTGACCCTTCA+GGG + chr6.1:74975647-74975666 MS.gene052431:CDS 50.0%
GATGTTATTGGTCCCCCTGA+CGG + chr6.1:74976165-74976184 MS.gene052431:CDS 50.0%
GTAACCAGGCCAGTTGCTAA+GGG + chr6.1:74976351-74976370 MS.gene052431:CDS 50.0%
GTCCCTACTCCACTTTCCAA+CGG + chr6.1:74975232-74975251 MS.gene052431:CDS 50.0%
TACCCGTTGGAAAGTGGAGT+AGG - chr6.1:74975238-74975257 None:intergenic 50.0%
TCCAATGGAGTTGACCCTTC+AGG + chr6.1:74975646-74975665 MS.gene052431:CDS 50.0%
TCCCTACTCCACTTTCCAAC+GGG + chr6.1:74975233-74975252 MS.gene052431:CDS 50.0%
TGTAACCAGGCCAGTTGCTA+AGG + chr6.1:74976350-74976369 MS.gene052431:CDS 50.0%
! AACTGGCCTGGTTACATCCA+AGG - chr6.1:74976346-74976365 None:intergenic 50.0%
! GGCCGACCAGAACATAGTTT+TGG + chr6.1:74976541-74976560 MS.gene052431:CDS 50.0%
! GGTCGGCCACAAAAGGTATA+TGG - chr6.1:74976529-74976548 None:intergenic 50.0%
! TGGACTGGACAATCCTCCAA+TGG + chr6.1:74976700-74976719 MS.gene052431:CDS 50.0%
ACATGTTCATCAGAGGCGGC+CGG + chr6.1:74975947-74975966 MS.gene052431:CDS 55.0%
AGGAAAAGACCACCGTCAGG+GGG - chr6.1:74976180-74976199 None:intergenic 55.0%
ATGTTAGGGTTCTCCGTCGC+AGG + chr6.1:74976729-74976748 MS.gene052431:CDS 55.0%
CCCTGAAGGGTCAACTCCAT+TGG - chr6.1:74975650-74975669 None:intergenic 55.0%
GAGGAAAAGACCACCGTCAG+GGG - chr6.1:74976181-74976200 None:intergenic 55.0%
GGAGGAAAAGACCACCGTCA+GGG - chr6.1:74976182-74976201 None:intergenic 55.0%
GGATCCATCAAAGATCCGGC+TGG + chr6.1:74975886-74975905 MS.gene052431:CDS 55.0%
TCACTTCGGCCCTTAGCAAC+TGG - chr6.1:74976363-74976382 None:intergenic 55.0%
TGCTAAGGGCCGAAGTGACT+TGG + chr6.1:74976365-74976384 MS.gene052431:CDS 55.0%
! AAAGTGGTTGGTGCCCCTGA+AGG - chr6.1:74975664-74975683 None:intergenic 55.0%
! AAGTGGTTGGTGCCCCTGAA+GGG - chr6.1:74975663-74975682 None:intergenic 55.0%
GAGGCGGCCGGATTATTGCA+AGG + chr6.1:74975959-74975978 MS.gene052431:CDS 60.0%
GGGAGGAAAAGACCACCGTC+AGG - chr6.1:74976183-74976202 None:intergenic 60.0%
GTTATTGGTCCCCCTGACGG+TGG + chr6.1:74976168-74976187 MS.gene052431:CDS 60.0%
TCGGCCCTTAGCAACTGGCC+TGG - chr6.1:74976358-74976377 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.1 gene 74975229 74976760 74975229 ID=MS.gene052431
chr6.1 mRNA 74975229 74976760 74975229 ID=MS.gene052431.t1;Parent=MS.gene052431
chr6.1 exon 74975229 74975254 74975229 ID=MS.gene052431.t1.exon1;Parent=MS.gene052431.t1
chr6.1 CDS 74975229 74975254 74975229 ID=cds.MS.gene052431.t1;Parent=MS.gene052431.t1
chr6.1 exon 74975642 74975708 74975642 ID=MS.gene052431.t1.exon2;Parent=MS.gene052431.t1
chr6.1 CDS 74975642 74975708 74975642 ID=cds.MS.gene052431.t1;Parent=MS.gene052431.t1
chr6.1 exon 74975820 74976386 74975820 ID=MS.gene052431.t1.exon3;Parent=MS.gene052431.t1
chr6.1 CDS 74975820 74976386 74975820 ID=cds.MS.gene052431.t1;Parent=MS.gene052431.t1
chr6.1 exon 74976503 74976760 74976503 ID=MS.gene052431.t1.exon4;Parent=MS.gene052431.t1
chr6.1 CDS 74976503 74976760 74976503 ID=cds.MS.gene052431.t1;Parent=MS.gene052431.t1
Gene Sequence

>MS.gene052431

ATGGTCCCTACTCCACTTTCCAACGGGTAAGTCAACACCATATCTAATCTTTTGGTCCCTACATCAAAATATATTTTTTGGCTTCTTCTCAGACTAAAATCCAATATAATTTCCTTTTTTGAATTTTCTAATGATCATTCCACATTCTTTACATTTGTTTTCATATATACTAGGAGGTAGTTTGAAGTATCAGATATGTGCATGTAAAGCTTTCCCATCTAATCATTATTTTGCTACTATAACACAATGAAAACTTTTTTTTTATAAAACTCATTCATGCACGTATTATTTATATTTTGTCTTAGTGATTTATGTTCCATATGTGGGTGTAAGTAGTATAGTTGATGCTTGGTAAGATAGATACATTTTGATTTATTTAACGAAGCTAAATATTTTATTGTCATTTTTTTTAGACAATCCAATGGAGTTGACCCTTCAGGGGCACCAACCACTTTTGATACCATGGTATATCCTGAACAAGTAAGGATCTTAAATAATATCTATTAAAATTTCATCTTATTGTAAATGAAACTCACTTCGATTGTTGTTTGTTGATCTTTTTATTTGTATCTTATATAAATTATAATATAGGACCGTATTGAAATCCCTCCTGAATTTGTCGTCGATTGGAAGGATAGCATCGCTGTACAAAATGTCGGATCCATCAAAGATCCGGCTGGAAATTTTTTTGGGATTTATACTCGAACTATTTTGTCTGACATGTTCATCAGAGGCGGCCGGATTATTGCAAGGTACTGTGGTTTCAATGAACCGCAGAAGGTAATTCTTGACTACAAGGTTAATGATAACCAGTTCAATATGCGAATTGTTGATGGAGAAGGGAACGACGTACCATATTTTGGATTCCATTATCCTGAAAATATTCATGCTAAGATACTTGCTAATCCAAACCTTCAAGTCAATCCGAGGTATGTTGATGTTATTGGTCCCCCTGACGGTGGTCTTTTCCTCCCCTTTGAGTTTGCAATGTTATGGATGGAAGAACTTAACGGTGTCAATGACGACAGCCTTATCCAATTTCTACAATTATCAGATGAACATAATGAGACTTCGGAATCTGATGAAGATGTTCCTGCTTTTGACTTTCAGTGGACCTTGGATGTAACCAGGCCAGTTGCTAAGGGCCGAAGTGACTTGGTTAGTACACACCATTAAAATCTATTTTTTATATTTGAAGACTTATGTTGAATTATTTAAGTTTAATTTATATATAATTATCCTGCATATTTAAACAAACATATTTATTTTTGTAGTATTTCCCCAAATCTGTAGTCAACCATATACCTTTTGTGGCCGACCAGAACATAGTTTTGGTTGATGACAACACAAATGAGCGATTCCCATGCAAAGTATCGATGGATACAACATCGGAAGAAGCAATTTACATAGGGAGTGGCTGGTATGAATGTGCCAAAAGTCTAAGATTGAAGACTGGTGACATGCTTTTCTTTGGACTGGACAATCCTCCAATGGAACTCAATGTTAGGGTTCTCCGTCGCAGGTCTCACTGA
Protein sequence

>MS.gene052431.t1

MVPTPLSNGQSNGVDPSGAPTTFDTMVYPEQDRIEIPPEFVVDWKDSIAVQNVGSIKDPAGNFFGIYTRTILSDMFIRGGRIIARYCGFNEPQKVILDYKVNDNQFNMRIVDGEGNDVPYFGFHYPENIHAKILANPNLQVNPRYVDVIGPPDGGLFLPFEFAMLWMEELNGVNDDSLIQFLQLSDEHNETSESDEDVPAFDFQWTLDVTRPVAKGRSDLYFPKSVVNHIPFVADQNIVLVDDNTNERFPCKVSMDTTSEEAIYIGSGWYECAKSLRLKTGDMLFFGLDNPPMELNVRVLRRRSH