AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052571

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052571.t1 MTR_4g091490 74.194 279 41 6 17 269 85 358 3.31e-125 360
MS.gene052571.t1 MTR_2g011660 40.809 272 131 11 17 269 85 345 6.78e-31 117
MS.gene052571.t1 MTR_2g089620 36.201 279 116 14 17 269 84 326 3.66e-29 112
MS.gene052571.t1 MTR_4g478180 41.304 138 69 4 4 140 75 201 4.40e-22 94.4
MS.gene052571.t1 MTR_5g007370 42.537 134 54 5 4 118 74 203 6.51e-21 90.1
MS.gene052571.t1 MTR_5g029840 56.098 82 30 2 4 82 74 152 1.50e-20 89.7
MS.gene052571.t1 MTR_3g074520 57.143 63 24 1 4 66 74 133 9.52e-19 84.7
MS.gene052571.t1 MTR_7g011170 64.151 53 19 0 14 66 81 133 1.47e-18 84.0
MS.gene052571.t1 MTR_4g073420 46.535 101 52 2 17 117 84 182 2.90e-18 82.8
MS.gene052571.t1 MTR_4g102380 78.049 41 9 0 21 61 88 128 4.13e-18 82.0
MS.gene052571.t1 MTR_4g082290 64.706 51 18 0 14 64 81 131 6.67e-18 82.8
MS.gene052571.t1 MTR_4g082230 64.706 51 18 0 14 64 81 131 7.63e-18 82.8
MS.gene052571.t1 MTR_6g009430 56.061 66 27 1 21 86 88 151 1.06e-17 81.6
MS.gene052571.t1 MTR_6g012690 56.338 71 31 0 14 84 81 151 1.62e-17 81.6
MS.gene052571.t1 MTR_7g076740 50.000 72 33 1 16 87 84 152 1.57e-16 78.2
MS.gene052571.t1 MTR_5g078140 75.610 41 10 0 21 61 88 128 2.35e-16 77.8
MS.gene052571.t1 MTR_8g095390 65.306 49 17 0 13 61 80 128 3.13e-16 77.4
MS.gene052571.t1 MTR_8g031360 68.889 45 14 0 17 61 86 130 4.19e-16 76.6
MS.gene052571.t1 MTR_5g042030 60.377 53 21 0 10 62 72 124 5.98e-16 76.3
MS.gene052571.t1 MTR_3g065440 77.500 40 9 0 21 60 88 127 1.07e-15 75.9
MS.gene052571.t1 MTR_3g097450 49.383 81 38 1 4 84 74 151 1.55e-15 75.1
MS.gene052571.t1 MTR_5g007310 65.909 44 15 0 27 70 2 45 2.76e-15 73.6
MS.gene052571.t1 MTR_5g079670 65.116 43 15 0 17 59 83 125 3.44e-15 72.8
MS.gene052571.t1 MTR_3g083540 66.667 45 15 0 17 61 113 157 4.28e-15 73.9
MS.gene052571.t1 MTR_4g485530 59.574 47 19 0 13 59 80 126 1.11e-14 71.2
MS.gene052571.t1 MTR_1g112760 63.415 41 15 0 21 61 89 129 1.25e-14 71.6
MS.gene052571.t1 MTR_3g461490 55.172 58 23 1 4 61 74 128 2.11e-14 72.4
MS.gene052571.t1 MTR_4g105130 63.415 41 15 0 21 61 88 128 4.07e-14 71.6
MS.gene052571.t1 MTR_3g077110 60.976 41 16 0 21 61 88 128 4.90e-14 71.2
MS.gene052571.t1 MTR_1g085640 62.222 45 17 0 17 61 84 128 8.99e-14 70.9
MS.gene052571.t1 MTR_3g045430 61.364 44 17 0 18 61 86 129 9.92e-14 70.1
MS.gene052571.t1 MTR_7g110830 60.000 45 18 0 17 61 84 128 3.00e-13 69.3
MS.gene052571.t1 MTR_1g085040 60.000 45 18 0 17 61 84 128 2.22e-12 66.6
MS.gene052571.t1 MTR_2g064160 54.545 44 20 0 17 60 83 126 7.28e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052571.t1 AT4G21440 42.545 275 119 10 21 269 88 349 1.21e-44 154
MS.gene052571.t1 AT5G54230 48.750 160 73 6 17 171 84 239 1.56e-31 119
MS.gene052571.t1 AT4G05100 37.091 275 113 8 17 269 85 321 7.04e-30 114
MS.gene052571.t1 AT5G16770 57.895 76 29 1 4 79 74 146 1.81e-21 92.0
MS.gene052571.t1 AT5G16770 57.895 76 29 1 4 79 74 146 1.81e-21 92.0
MS.gene052571.t1 AT5G16770 57.895 76 29 1 4 79 74 146 1.96e-21 92.4
MS.gene052571.t1 AT5G16770 57.895 76 29 1 4 79 74 146 1.96e-21 92.4
MS.gene052571.t1 AT3G02940 66.154 65 19 1 4 68 74 135 2.56e-21 91.7
MS.gene052571.t1 AT4G28110 83.333 42 7 0 21 62 4 45 3.26e-21 89.0
MS.gene052571.t1 AT3G02940 66.154 65 19 1 4 68 74 135 3.28e-21 91.3
MS.gene052571.t1 AT3G02940 66.154 65 19 1 4 68 74 135 3.28e-21 91.3
MS.gene052571.t1 AT4G17785 68.333 60 17 1 16 75 84 141 1.36e-20 90.1
MS.gene052571.t1 AT4G17785 68.333 60 17 1 16 75 84 141 1.62e-20 90.1
MS.gene052571.t1 AT4G28110 78.261 46 10 0 17 62 84 129 4.83e-20 87.4
MS.gene052571.t1 AT5G15310 69.388 49 14 1 14 62 3 50 1.22e-19 85.9
MS.gene052571.t1 AT5G10280 58.730 63 23 1 4 66 74 133 3.50e-19 85.9
MS.gene052571.t1 AT4G22680 82.927 41 7 0 21 61 88 128 2.28e-18 82.8
MS.gene052571.t1 AT4G12350 41.667 96 53 1 17 112 84 176 2.87e-18 82.8
MS.gene052571.t1 AT5G65230 60.656 61 21 1 4 64 74 131 3.60e-18 82.8
MS.gene052571.t1 AT1G34670 58.730 63 23 1 4 66 74 133 3.87e-18 83.2
MS.gene052571.t1 AT4G34990 73.333 45 12 0 17 61 84 128 4.58e-18 82.0
MS.gene052571.t1 AT5G15310 65.306 49 17 0 14 62 81 129 7.65e-18 82.0
MS.gene052571.t1 AT3G13540 73.333 45 12 0 17 61 95 139 8.20e-18 80.9
MS.gene052571.t1 AT5G15310 65.306 49 17 0 14 62 81 129 8.31e-18 80.9
MS.gene052571.t1 AT5G15310 65.306 49 17 0 14 62 81 129 8.83e-18 81.6
MS.gene052571.t1 AT3G01140 54.930 71 30 1 14 84 81 149 2.70e-17 80.9
MS.gene052571.t1 AT3G01140 54.930 71 30 1 14 84 124 192 5.47e-17 80.1
MS.gene052571.t1 AT4G38620 71.111 45 13 0 17 61 84 128 7.13e-17 79.0
MS.gene052571.t1 AT1G18570 72.340 47 13 0 13 59 81 127 8.41e-17 79.3
MS.gene052571.t1 AT5G61420 75.610 41 10 0 21 61 9 49 1.85e-16 77.8
MS.gene052571.t1 AT1G66230 75.610 41 10 0 21 61 88 128 2.04e-16 77.4
MS.gene052571.t1 AT5G16600 75.610 41 10 0 21 61 88 128 2.15e-16 78.2
MS.gene052571.t1 AT2G16720 72.727 44 12 0 17 60 84 127 3.41e-16 76.6
MS.gene052571.t1 AT1G35515 61.404 57 19 1 4 60 74 127 5.70e-16 75.1
MS.gene052571.t1 AT4G09460 64.286 56 17 1 4 59 74 126 7.22e-16 75.1
MS.gene052571.t1 AT1G22640 50.000 74 27 1 17 90 27 90 7.69e-16 74.3
MS.gene052571.t1 AT1G22640 44.706 85 36 2 6 90 2 75 8.05e-16 73.9
MS.gene052571.t1 AT3G61250 63.043 46 17 0 16 61 83 128 1.10e-15 75.9
MS.gene052571.t1 AT5G61420 75.610 41 10 0 21 61 88 128 1.11e-15 76.3
MS.gene052571.t1 AT1G57560 47.887 71 36 1 17 86 84 154 1.55e-15 75.1
MS.gene052571.t1 AT1G22640 50.000 74 27 1 17 90 84 147 3.04e-15 73.9
MS.gene052571.t1 AT1G57560 62.222 45 17 0 17 61 84 128 5.55e-15 73.9
MS.gene052571.t1 AT5G07700 73.171 41 11 0 21 61 88 128 6.23e-15 73.9
MS.gene052571.t1 AT5G07700 73.171 41 11 0 21 61 88 128 6.23e-15 73.9
MS.gene052571.t1 AT5G62320 72.500 40 11 0 21 60 96 135 6.58e-15 72.8
MS.gene052571.t1 AT5G26660 68.293 41 13 0 21 61 57 97 9.73e-15 73.2
MS.gene052571.t1 AT5G26660 68.293 41 13 0 21 61 88 128 1.82e-14 72.8
MS.gene052571.t1 AT5G07690 73.171 41 11 0 21 61 88 128 2.11e-14 72.4
MS.gene052571.t1 AT5G14340 37.000 100 62 1 16 115 5 103 2.58e-14 70.1
MS.gene052571.t1 AT5G56110 68.293 41 13 0 21 61 88 128 4.70e-14 71.2
MS.gene052571.t1 AT5G60890 45.070 71 37 1 16 84 83 153 5.39e-14 70.9
MS.gene052571.t1 AT1G74080 62.000 50 19 0 21 70 88 137 6.72e-14 70.9
MS.gene052571.t1 AT1G09540 63.043 46 17 0 16 61 83 128 1.69e-13 70.1
MS.gene052571.t1 AT1G74430 45.763 59 32 0 3 61 1 59 1.76e-12 65.1
MS.gene052571.t1 AT3G12720 57.447 47 20 0 14 60 91 137 2.48e-12 66.2
MS.gene052571.t1 AT5G14340 37.895 95 58 1 21 115 100 193 5.78e-12 64.7
MS.gene052571.t1 AT1G71030 47.917 48 25 0 13 60 34 81 6.17e-12 63.2
MS.gene052571.t1 AT1G71030 47.917 48 25 0 13 60 48 95 7.63e-12 63.2
MS.gene052571.t1 AT1G74430 52.174 46 22 0 16 61 83 128 8.12e-12 64.3
MS.gene052571.t1 AT4G01680 65.000 40 14 0 21 60 88 127 1.54e-11 63.2
MS.gene052571.t1 AT4G01680 56.522 46 20 0 14 59 81 126 1.66e-11 63.9
MS.gene052571.t1 AT4G01680 66.667 39 13 0 21 59 100 138 1.81e-11 63.9
MS.gene052571.t1 AT3G28470 65.217 46 16 0 21 66 88 133 2.38e-11 63.5

Find 39 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATCCACGCCTTGATCTTT+TGG 0.233178 4.3:-22629717 MS.gene052571:CDS
TCATGCAAATGATTTAATTA+TGG 0.238692 4.3:-22629276 MS.gene052571:CDS
TATTCGTCAAATTCGAATTC+TGG 0.271160 4.3:+22629207 None:intergenic
GGTGGTCTTCAATTGCTTCT+AGG 0.280586 4.3:-22629832 MS.gene052571:CDS
ATTTCATTGTCTGTTCTTCC+TGG 0.287218 4.3:+22629806 None:intergenic
ATGAACATCCAAAGGCTAAT+TGG 0.305595 4.3:-22629644 MS.gene052571:CDS
ACAATGAAATCAAGAATTAT+TGG 0.309694 4.3:-22629793 MS.gene052571:CDS
AATTGTTGGAAGCCAGATTC+AGG 0.314520 4.3:+22629392 None:intergenic
CTTCAAATTTAATGCTATCA+TGG 0.348246 4.3:+22630837 None:intergenic
ATTATTGGAACACTCACATT+AGG 0.361810 4.3:-22629778 MS.gene052571:CDS
AATGCAAATCATTTAATTGT+TGG 0.407951 4.3:+22629378 None:intergenic
TCGAATTTGACGAATATCAA+TGG 0.430482 4.3:-22629200 MS.gene052571:CDS
TGACGAGTATAGATTCTCAT+TGG 0.444633 4.3:+22629421 None:intergenic
GAACACAGAGATTCTAAAGT+TGG 0.445522 4.3:-22629606 MS.gene052571:CDS
TTTCCTATCTCATATAGCTT+AGG 0.452549 4.3:-22629125 MS.gene052571:CDS
TAGGAAAAGACTGTTAAGAA+TGG 0.463534 4.3:-22629759 MS.gene052571:CDS
ACACCTAAGCTATATGAGAT+AGG 0.463774 4.3:+22629122 None:intergenic
ATTGTGTACCAATTAGCCTT+TGG 0.464975 4.3:+22629636 None:intergenic
ATGAATTTATGATAGAAGAT+AGG 0.469498 4.3:+22629693 None:intergenic
TTAAATGATTTGCATTGCAA+TGG 0.472550 4.3:-22629371 MS.gene052571:CDS
TTTGAAGAGTGTGTTCTGTT+TGG 0.481306 4.3:-22630821 MS.gene052571:CDS
CCAAGTGTGAGCTAATTTGT+TGG 0.490696 4.3:+22629528 None:intergenic
AGGAAAAGACTGTTAAGAAT+GGG 0.497473 4.3:-22629758 MS.gene052571:CDS
TCAATTGCTTCTAGGTTACC+AGG 0.516154 4.3:-22629824 MS.gene052571:CDS
CCAACAAATTAGCTCACACT+TGG 0.521089 4.3:-22629528 MS.gene052571:CDS
GTTCATTTGTGATGAGGTAG+AGG 0.528903 4.3:+22629661 None:intergenic
AAGATAGGTCCAAAAGATCA+AGG 0.542013 4.3:+22629708 None:intergenic
GTTGTTGTTATTGCAATGAA+AGG 0.546533 4.3:+22629229 None:intergenic
ACTGAGTGTGTTTGAGTGAA+AGG 0.550456 4.3:+22629449 None:intergenic
AGGCGTGGATTATGTGTCAC+AGG 0.568066 4.3:+22629728 None:intergenic
TTGGATGTTCATTTGTGATG+AGG 0.578710 4.3:+22629655 None:intergenic
GAACTTGAGTAGTTATAGCA+TGG 0.608371 4.3:+22629305 None:intergenic
CATCACAAATGAACATCCAA+AGG 0.625838 4.3:-22629652 MS.gene052571:CDS
GTTGAAGTTTCTGATATGTG+AGG 0.628864 4.3:+22629251 None:intergenic
TGCTTCAAGTAATGTAACAG+AGG 0.642911 4.3:-22629345 MS.gene052571:CDS
AGGTCCAAAAGATCAAGGCG+TGG 0.692260 4.3:+22629713 None:intergenic
GAATTATTATGACCACAAGG+TGG 0.694409 4.3:+22629476 None:intergenic
ATTGAATTATTATGACCACA+AGG 0.710509 4.3:+22629473 None:intergenic
ATGTTGTTGAATCCACCTTG+TGG 0.743965 4.3:-22629488 MS.gene052571:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATAATGTTGATTATTT+TGG + chr4.3:22629746-22629765 None:intergenic 10.0%
!! ATATATTGAAGTAAAATGTT+TGG - chr4.3:22629760-22629779 MS.gene052571:CDS 15.0%
!! AATGCAAATCATTTAATTGT+TGG + chr4.3:22630581-22630600 None:intergenic 20.0%
!! ACAATGAAATCAAGAATTAT+TGG - chr4.3:22630163-22630182 MS.gene052571:intron 20.0%
!! ATAGCTTATATTCAAAAACA+TGG - chr4.3:22629622-22629641 MS.gene052571:CDS 20.0%
!! ATGAATTTATGATAGAAGAT+AGG + chr4.3:22630266-22630285 None:intergenic 20.0%
!! GCTAATATTAAACTAATAGA+TGG + chr4.3:22629470-22629489 None:intergenic 20.0%
!! GTGGTATAAATAGAAATTAA+GGG + chr4.3:22629342-22629361 None:intergenic 20.0%
!! TATATTCAAAAACATGGTTA+TGG - chr4.3:22629628-22629647 MS.gene052571:CDS 20.0%
!! TCATGCAAATGATTTAATTA+TGG - chr4.3:22630680-22630699 MS.gene052571:intron 20.0%
!! TGTGGTATAAATAGAAATTA+AGG + chr4.3:22629343-22629362 None:intergenic 20.0%
!! TTTATCACAAGTGATTAAAA+TGG - chr4.3:22629158-22629177 MS.gene052571:CDS 20.0%
!!! ATTTTTGTGACTAAACTATA+TGG - chr4.3:22630087-22630106 MS.gene052571:intron 20.0%
!!! CTTTTGTTTTACATGAAATA+TGG + chr4.3:22629198-22629217 None:intergenic 20.0%
!!! GAATATAAGCTATAAGTTTT+TGG + chr4.3:22629615-22629634 None:intergenic 20.0%
! ATATGATATGTCTGAAAAGA+GGG + chr4.3:22629427-22629446 None:intergenic 25.0%
! ATTGAATTATTATGACCACA+AGG + chr4.3:22630486-22630505 None:intergenic 25.0%
! CTTCAAATTTAATGCTATCA+TGG + chr4.3:22629122-22629141 None:intergenic 25.0%
!! TTAAATGATTTGCATTGCAA+TGG - chr4.3:22630585-22630604 MS.gene052571:intron 25.0%
!!! TATATGGTTTTGAATTGTGT+AGG - chr4.3:22630103-22630122 MS.gene052571:intron 25.0%
AACTATAGCACATACTACTA+TGG - chr4.3:22629525-22629544 MS.gene052571:CDS 30.0%
ACTATAGCACATACTACTAT+GGG - chr4.3:22629526-22629545 MS.gene052571:CDS 30.0%
AGGAAAAGACTGTTAAGAAT+GGG - chr4.3:22630198-22630217 MS.gene052571:intron 30.0%
ATTATTGGAACACTCACATT+AGG - chr4.3:22630178-22630197 MS.gene052571:intron 30.0%
GATATGATATGTCTGAAAAG+AGG + chr4.3:22629428-22629447 None:intergenic 30.0%
GTGATGATTTGATAAGAAGA+GGG - chr4.3:22629254-22629273 MS.gene052571:CDS 30.0%
TAGGAAAAGACTGTTAAGAA+TGG - chr4.3:22630197-22630216 MS.gene052571:intron 30.0%
TATTCGTCAAATTCGAATTC+TGG + chr4.3:22630752-22630771 None:intergenic 30.0%
TCGAATTTGACGAATATCAA+TGG - chr4.3:22630756-22630775 MS.gene052571:intron 30.0%
TTTCCTATCTCATATAGCTT+AGG - chr4.3:22630831-22630850 MS.gene052571:CDS 30.0%
! CAACGTAGAGAAATTTTACT+AGG - chr4.3:22629232-22629251 MS.gene052571:CDS 30.0%
! CCATTATTCTTTTCACAACA+AGG + chr4.3:22629559-22629578 None:intergenic 30.0%
! CCTTGTTGTGAAAAGAATAA+TGG - chr4.3:22629556-22629575 MS.gene052571:CDS 30.0%
! GTTGTTGTTATTGCAATGAA+AGG + chr4.3:22630730-22630749 None:intergenic 30.0%
! TAGTATGTGCTATAGTTCTA+AGG + chr4.3:22629522-22629541 None:intergenic 30.0%
!! TATAGTTCTAAGGTAAGTGA+AGG + chr4.3:22629512-22629531 None:intergenic 30.0%
!!! GTTATTTTTGTTTGAGTTGC+AGG - chr4.3:22629782-22629801 MS.gene052571:CDS 30.0%
AAAAACATGGTTATGGCAAC+TGG - chr4.3:22629635-22629654 MS.gene052571:CDS 35.0%
AAGAATAATGGCCTCAAAAG+AGG - chr4.3:22629568-22629587 MS.gene052571:CDS 35.0%
AAGACACAGATGAAGTCAAA+TGG + chr4.3:22629318-22629337 None:intergenic 35.0%
AAGATAGGTCCAAAAGATCA+AGG + chr4.3:22630251-22630270 None:intergenic 35.0%
AATCGTCCTCTCTTTATATC+CGG + chr4.3:22629860-22629879 None:intergenic 35.0%
ACACCTAAGCTATATGAGAT+AGG + chr4.3:22630837-22630856 None:intergenic 35.0%
ATGAACATCCAAAGGCTAAT+TGG - chr4.3:22630312-22630331 MS.gene052571:intron 35.0%
ATGTATGTACCAGCATTCTT+TGG + chr4.3:22629670-22629689 None:intergenic 35.0%
ATTCATCTGCATAGCATTCT+TGG - chr4.3:22629905-22629924 MS.gene052571:intron 35.0%
ATTTCATTGTCTGTTCTTCC+TGG + chr4.3:22630153-22630172 None:intergenic 35.0%
CATCACAAATGAACATCCAA+AGG - chr4.3:22630304-22630323 MS.gene052571:intron 35.0%
CTCAATGGAACTTATTGTTG+TGG + chr4.3:22629361-22629380 None:intergenic 35.0%
GAACTTGAGTAGTTATAGCA+TGG + chr4.3:22630654-22630673 None:intergenic 35.0%
GAATTATTATGACCACAAGG+TGG + chr4.3:22630483-22630502 None:intergenic 35.0%
GATACTAAGACTCAACTCAA+TGG + chr4.3:22629376-22629395 None:intergenic 35.0%
GGTGATGATTTGATAAGAAG+AGG - chr4.3:22629253-22629272 MS.gene052571:CDS 35.0%
GTTGAAGTTTCTGATATGTG+AGG + chr4.3:22630708-22630727 None:intergenic 35.0%
TGACGAGTATAGATTCTCAT+TGG + chr4.3:22630538-22630557 None:intergenic 35.0%
TGCTTCAAGTAATGTAACAG+AGG - chr4.3:22630611-22630630 MS.gene052571:intron 35.0%
TGTATGTACCAGCATTCTTT+GGG + chr4.3:22629669-22629688 None:intergenic 35.0%
TTGGATGTTCATTTGTGATG+AGG + chr4.3:22630304-22630323 None:intergenic 35.0%
! CAGATGAAGTCAAATGGATT+TGG + chr4.3:22629312-22629331 None:intergenic 35.0%
! TTTGAAGAGTGTGTTCTGTT+TGG - chr4.3:22629135-22629154 MS.gene052571:CDS 35.0%
!! ATTGTGTACCAATTAGCCTT+TGG + chr4.3:22630323-22630342 None:intergenic 35.0%
!! GAACACAGAGATTCTAAAGT+TGG - chr4.3:22630350-22630369 MS.gene052571:intron 35.0%
!!! ATGGTTTTGAATTGTGTAGG+TGG - chr4.3:22630106-22630125 MS.gene052571:intron 35.0%
!!! TCGTCATTTTTACCTGAATC+TGG - chr4.3:22630552-22630571 MS.gene052571:intron 35.0%
AATTGTTGGAAGCCAGATTC+AGG + chr4.3:22630567-22630586 None:intergenic 40.0%
ACTGAGTGTGTTTGAGTGAA+AGG + chr4.3:22630510-22630529 None:intergenic 40.0%
AGATTACTCCCAAAGAATGC+TGG - chr4.3:22629658-22629677 MS.gene052571:CDS 40.0%
ATGTTGTTGAATCCACCTTG+TGG - chr4.3:22630468-22630487 MS.gene052571:intron 40.0%
CCAACAAATTAGCTCACACT+TGG - chr4.3:22630428-22630447 MS.gene052571:intron 40.0%
CGTAGCTAAAGAGACAACAA+AGG - chr4.3:22629280-22629299 MS.gene052571:CDS 40.0%
GCTAAAGAGACAACAAAGGA+CGG - chr4.3:22629284-22629303 MS.gene052571:CDS 40.0%
GTTCATTTGTGATGAGGTAG+AGG + chr4.3:22630298-22630317 None:intergenic 40.0%
GTTGCAGGATTACAAAGATG+TGG - chr4.3:22629797-22629816 MS.gene052571:CDS 40.0%
TCAATTGCTTCTAGGTTACC+AGG - chr4.3:22630132-22630151 MS.gene052571:intron 40.0%
TCTCTACGTTGTTTGTGTAC+AGG + chr4.3:22629223-22629242 None:intergenic 40.0%
! CCAAGTGTGAGCTAATTTGT+TGG + chr4.3:22630431-22630450 None:intergenic 40.0%
! TAATCCACGCCTTGATCTTT+TGG - chr4.3:22630239-22630258 MS.gene052571:intron 40.0%
GGTGGTCTTCAATTGCTTCT+AGG - chr4.3:22630124-22630143 MS.gene052571:intron 45.0%
GTCCTCTCTTTATATCCGGT+CGG + chr4.3:22629856-22629875 None:intergenic 45.0%
TGGTCAATGAGTTTGCGTGA+AGG + chr4.3:22629450-22629469 None:intergenic 45.0%
TTGGACAAACTATCTCCGAC+CGG - chr4.3:22629838-22629857 MS.gene052571:intron 45.0%
! GTCGGAGATAGTTTGTCCAA+CGG + chr4.3:22629838-22629857 None:intergenic 45.0%
AGGTCCAAAAGATCAAGGCG+TGG + chr4.3:22630246-22630265 None:intergenic 50.0%
ATGGCCTCAAAAGAGGACCA+TGG - chr4.3:22629575-22629594 MS.gene052571:CDS 50.0%
CTCCGACCGGATATAAAGAG+AGG - chr4.3:22629851-22629870 MS.gene052571:intron 50.0%
GAAAGAGTTGTCGTCTCCGT+TGG - chr4.3:22629819-22629838 MS.gene052571:CDS 50.0%
! AGGCGTGGATTATGTGTCAC+AGG + chr4.3:22630231-22630250 None:intergenic 50.0%
AAGAGGACCATGGACGCAAG+AGG - chr4.3:22629585-22629604 MS.gene052571:CDS 55.0%
TGGTCTTCCTCTTGCGTCCA+TGG + chr4.3:22629595-22629614 None:intergenic 55.0%
! GCGTCCATGGTCCTCTTTTG+AGG + chr4.3:22629582-22629601 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 22629106 22630872 22629106 ID=MS.gene052571
chr4.3 mRNA 22629106 22630872 22629106 ID=MS.gene052571.t1;Parent=MS.gene052571
chr4.3 exon 22630811 22630872 22630811 ID=MS.gene052571.t1.exon1;Parent=MS.gene052571.t1
chr4.3 CDS 22630811 22630872 22630811 ID=cds.MS.gene052571.t1;Parent=MS.gene052571.t1
chr4.3 exon 22629106 22629853 22629106 ID=MS.gene052571.t1.exon2;Parent=MS.gene052571.t1
chr4.3 CDS 22629106 22629853 22629106 ID=cds.MS.gene052571.t1;Parent=MS.gene052571.t1
Gene Sequence

>MS.gene052571

ATGATGCTTGAATCCATGATAGCATTAAATTTGAAGAGTGTGTTCTGTTTGGTTTATCACAAGTGATTAAAATGGATGATAAAATTTAACCATATTTCATGTAAAACAAAAGAACCTGTACACAAACAACGTAGAGAAATTTTACTAGGTGATGATTTGATAAGAAGAGGGACACGTAGCTAAAGAGACAACAAAGGACGGCACCAAATCCATTTGACTTCATCTGTGTCTTACCCTTAATTTCTATTTATACCACAACAATAAGTTCCATTGAGTTGAGTCTTAGTATCTCTATTTTCTCACGTCTTAAAAACTTCTCCCTCTTTTCAGACATATCATATCCTTCACGCAAACTCATTGACCATCTATTAGTTTAATATTAGCTCAACAATTAACTTCAAAGCCTTCACTTACCTTAGAACTATAGCACATACTACTATGGGAAGAGCACCTTGTTGTGAAAAGAATAATGGCCTCAAAAGAGGACCATGGACGCAAGAGGAAGACCAAAAACTTATAGCTTATATTCAAAAACATGGTTATGGCAACTGGAGATTACTCCCAAAGAATGCTGGTACATACATATATACATAAATACATACACATGTGTTATGAATCTTCAAATTAATTATTTCTACCAAAATAATCAACATTATATATTGAAGTAAAATGTTTGGTTATTTTTGTTTGAGTTGCAGGATTACAAAGATGTGGAAAGAGTTGTCGTCTCCGTTGGACAAACTATCTCCGACCGGATATAAAGAGAGGACGATTCTCTTTTGAAGAAGAAGAAACAATAATTCATCTGCATAGCATTCTTGGCAACAAGTAATAATTAAGTATCTTAGTATATAATTTTTTTAATCAATTTAACTACACACATGTGTAACAAATTATATCTATGAGTCAAATACTAGTACATAATAATTTTCATTATGATTAAGATAGATACTATATACTATATATAGAATAATAGATATGATTTTTGTGACTAAACTATATGGTTTTGAATTGTGTAGGTGGTCTTCAATTGCTTCTAGGTTACCAGGAAGAACAGACAATGAAATCAAGAATTATTGGAACACTCACATTAGGAAAAGACTGTTAAGAATGGGAATTGATCCTGTGACACATAATCCACGCCTTGATCTTTTGGACCTATCTTCTATCATAAATTCATCTCTCTATGCCTCTACCTCATCACAAATGAACATCCAAAGGCTAATTGGTACACAATCAATAGTGAACACAGAGATTCTAAAGTTGGCTTCATCACTCTTCTCTTCTCAAAATGTACAAGAGAATTATCAAATTGATCAAAACCAACAAATTAGCTCACACTTGGATCAAGAAGCTTGCAACATGTTGTTGAATCCACCTTGTGGTCATAATAATTCAATGCCTTTCACTCAAACACACTCAGTTGAATCCAATGAGAATCTATACTCGTCATTTTTACCTGAATCTGGCTTCCAACAATTAAATGATTTGCATTGCAATGGAATTGCTTCAAGTAATGTAACAGAGGATTTTGTTCATCAATTACCATGCTATAACTACTCAAGTTCTGATTATCATGCAAATGATTTAATTATGGAGCCTCACATATCAGAAACTTCAACCTTTCATTGCAATAACAACAACCAGAATTCGAATTTGACGAATATCAATGGAAGCAGTAATGAAGATGAGACAGAGAGCTATGTTAGCAGCAACATATTTGAATTTCCTATCTCATATAGCTTAGGTGTGAATGAGTTCATGTAA
Protein sequence

>MS.gene052571.t1

MMLESMIALNLKSVFCLVYHKWSSIASRLPGRTDNEIKNYWNTHIRKRLLRMGIDPVTHNPRLDLLDLSSIINSSLYASTSSQMNIQRLIGTQSIVNTEILKLASSLFSSQNVQENYQIDQNQQISSHLDQEACNMLLNPPCGHNNSMPFTQTHSVESNENLYSSFLPESGFQQLNDLHCNGIASSNVTEDFVHQLPCYNYSSSDYHANDLIMEPHISETSTFHCNNNNQNSNLTNINGSSNEDETESYVSSNIFEFPISYSLGVNEFM