AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052619


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052619.t1 MTR_3g075270 91.845 233 19 0 10 242 1 233 3.53e-162 451
MS.gene052619.t1 MTR_4g104140 55.159 252 103 3 1 242 36 287 5.50e-99 293
MS.gene052619.t1 MTR_4g104140 59.420 207 81 2 39 242 11 217 2.90e-88 263
MS.gene052619.t1 MTR_5g015680 40.081 247 141 2 3 242 89 335 2.22e-61 197
MS.gene052619.t1 MTR_5g015680 40.081 247 141 2 3 242 128 374 6.18e-61 197
MS.gene052619.t1 MTR_5g015680 40.000 240 141 2 6 242 1 240 1.89e-60 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052619.t1 AT3G48360 48.276 261 115 3 2 242 55 315 2.56e-77 237
MS.gene052619.t1 AT3G48360 48.092 262 116 3 1 242 54 315 3.23e-77 237
MS.gene052619.t1 AT5G63160 48.837 258 115 4 2 242 46 303 2.28e-76 233
MS.gene052619.t1 AT5G63160 48.837 258 115 4 2 242 46 303 7.22e-76 234
MS.gene052619.t1 AT3G48360 52.153 209 90 2 44 242 1 209 3.63e-67 208
MS.gene052619.t1 AT5G63160 52.133 211 93 3 40 242 8 218 1.56e-66 207
MS.gene052619.t1 AT1G05690 40.800 250 138 5 1 242 74 321 2.16e-57 186
MS.gene052619.t1 AT4G37610 39.759 249 139 6 3 242 77 323 2.59e-55 181
MS.gene052619.t1 AT5G67480 38.400 250 144 5 2 242 81 329 1.69e-52 174
MS.gene052619.t1 AT5G67480 38.400 250 144 5 2 242 81 329 1.69e-52 174
MS.gene052619.t1 AT5G67480 38.400 250 144 5 2 242 92 340 1.91e-52 174

Find 61 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACCGCTTCAAAATTAT+TGG 0.124222 3.1:+62085201 None:intergenic
CTCCACATGTCAAGCTTTGC+AGG 0.259755 3.1:-62084858 MS.gene052619:CDS
AATGCACCTTTGCTTTAGTT+TGG 0.301923 3.1:+62085330 None:intergenic
CATGCAAGGTTCCTCTTTGC+AGG 0.308788 3.1:-62084721 None:intergenic
TACATTTCATCTACACTTCC+AGG 0.332926 3.1:-62085629 MS.gene052619:intron
ACACACTTTAAGTAGAGATT+TGG 0.333717 3.1:+62085235 None:intergenic
CATTCCCCGTGGTCATGAAT+TGG 0.355331 3.1:+62085296 None:intergenic
ATCATATGATGTGGAGATTA+CGG 0.355897 3.1:-62084900 MS.gene052619:CDS
AAGTGAAGCTGTACAATGTT+TGG 0.366740 3.1:-62084957 MS.gene052619:CDS
ATGCACCTTTGCTTTAGTTT+GGG 0.372763 3.1:+62085331 None:intergenic
AACACTTTACCACATGTAAT+AGG 0.401202 3.1:-62084826 MS.gene052619:CDS
TCATATGATGTGGAGATTAC+GGG 0.403872 3.1:-62084899 MS.gene052619:CDS
TGGGACTATGCAGGTGCAGC+AGG 0.411993 3.1:-62085411 MS.gene052619:intron
GTCCCCAGTTCTAAAAGAAA+TGG 0.455888 3.1:-62085712 MS.gene052619:CDS
GAGGAACCTTGCATGCATCT+TGG 0.458524 3.1:+62084729 None:intergenic
GAAGAGAGGAAATAAACATA+GGG 0.473780 3.1:-62085005 MS.gene052619:CDS
AGAAGAGAGGAAATAAACAT+AGG 0.475467 3.1:-62085006 MS.gene052619:CDS
AACGGTAACGTTAAAATATG+TGG 0.514946 3.1:-62085681 MS.gene052619:CDS
AAGGCGATGGCTGCATTGGT+TGG 0.517717 3.1:+62085654 None:intergenic
TCCACATGTCAAGCTTTGCA+GGG 0.524594 3.1:-62084857 MS.gene052619:CDS
TTGATCCAATTCATGACCAC+GGG 0.542240 3.1:-62085301 MS.gene052619:CDS
TATTGCATAAACATGACCCT+TGG 0.542900 3.1:-62085159 MS.gene052619:CDS
TACAAAGGCGATGGCTGCAT+TGG 0.548314 3.1:+62085650 None:intergenic
TCTCAACAAAGAACGAGAAT+CGG 0.559033 3.1:-62085116 MS.gene052619:intron
ATTCTCGTTCTTTGTTGAGA+CGG 0.562147 3.1:+62085119 None:intergenic
CCACATGTCAAGCTTTGCAG+GGG 0.564441 3.1:-62084856 MS.gene052619:CDS
ATGATGTCAACTTCGAGCCA+AGG 0.576653 3.1:+62085142 None:intergenic
ATTCATGACCACGGGGAATG+TGG 0.583872 3.1:-62085293 MS.gene052619:CDS
TTGGAGCATATATGTACAGA+AGG 0.585859 3.1:-62084938 MS.gene052619:CDS
TGTAATAGGAGACTCGAGAG+AGG 0.587137 3.1:-62084812 MS.gene052619:CDS
TGATGTCAACTTCGAGCCAA+GGG 0.587603 3.1:+62085143 None:intergenic
AAAGAAATGGTAACGTCCAA+CGG 0.587929 3.1:-62085699 MS.gene052619:CDS
TGTGGTGGACGTGTTGCACT+TGG 0.589909 3.1:-62085275 MS.gene052619:CDS
CCCCTGCAAAGCTTGACATG+TGG 0.598401 3.1:+62084856 None:intergenic
TGTTTCGCATACCTGCAAAG+AGG 0.600432 3.1:+62084710 None:intergenic
CAATGCCCAAACTAAAGCAA+AGG 0.601233 3.1:-62085336 MS.gene052619:CDS
GAAGCGGTGAAGGAAACAGA+GGG 0.603513 3.1:-62085190 MS.gene052619:CDS
CAACGTGGCATCATATGATG+TGG 0.607548 3.1:-62084909 MS.gene052619:CDS
CTCGAGTCTCCTATTACATG+TGG 0.612075 3.1:+62084817 None:intergenic
GGAGACTCGAGAGAGGGTGT+AGG 0.617740 3.1:-62084805 MS.gene052619:CDS
GAGAGGAAATAAACATAGGG+AGG 0.621519 3.1:-62085002 MS.gene052619:CDS
CTTGATCCAATTCATGACCA+CGG 0.630679 3.1:-62085302 MS.gene052619:CDS
CATATGATGTGGAGATTACG+GGG 0.632166 3.1:-62084898 MS.gene052619:CDS
CATGACCACGGGGAATGTGG+TGG 0.632280 3.1:-62085290 MS.gene052619:CDS
GTAGGTGTTGTAAGTCTATG+TGG 0.636359 3.1:-62084787 MS.gene052619:CDS
GTGAAACAGAGGAAGAAGAG+AGG 0.641073 3.1:-62085019 MS.gene052619:intron
CGGTGAAGGAAACAGAGGGG+TGG 0.647649 3.1:-62085186 MS.gene052619:CDS
AGTGTAGATGAAATGTACAA+AGG 0.647924 3.1:+62085635 None:intergenic
GGGACTATGCAGGTGCAGCA+GGG 0.659269 3.1:-62085410 MS.gene052619:intron
CATGTGGAGAATTTAGTACA+CGG 0.664071 3.1:+62084872 None:intergenic
CACATCATATGATGCCACGT+TGG 0.666948 3.1:+62084910 None:intergenic
GATGAAATGTACAAAGGCGA+TGG 0.667885 3.1:+62085641 None:intergenic
GTAATAGGAGACTCGAGAGA+GGG 0.668774 3.1:-62084811 MS.gene052619:CDS
TGAAGCGGTGAAGGAAACAG+AGG 0.678822 3.1:-62085191 MS.gene052619:CDS
TACAGAAGGTTGCACCAACG+TGG 0.692512 3.1:-62084924 MS.gene052619:CDS
CACGTCCACCACATTCCCCG+TGG 0.707824 3.1:+62085285 None:intergenic
AGACGTGCGAGAGTACGAGA+AGG 0.721408 3.1:+62085362 None:intergenic
ACTATGCAGGTGCAGCAGGG+AGG 0.723689 3.1:-62085407 MS.gene052619:intron
ATGATGTGGAGATTACGGGG+AGG 0.765492 3.1:-62084895 MS.gene052619:CDS
AAGCGGTGAAGGAAACAGAG+GGG 0.769201 3.1:-62085189 MS.gene052619:CDS
TGATCCAATTCATGACCACG+GGG 0.774666 3.1:-62085300 MS.gene052619:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATTTCTTTAATAAGT+TGG - chr3.1:62085393-62085412 MS.gene052619:CDS 10.0%
!!! AATTGAATTAACAAATTTTT+TGG - chr3.1:62084884-62084903 MS.gene052619:CDS 10.0%
!! GGTCTAAATACATCAATTTT+CGG - chr3.1:62084905-62084924 MS.gene052619:CDS 25.0%
!!! AGTTACCAATAATTTTGAAG+CGG - chr3.1:62085236-62085255 MS.gene052619:CDS 25.0%
!!! TTACCATTTCTTTTAGAACT+GGG + chr3.1:62084736-62084755 None:intergenic 25.0%
!!! TTCTTTAATAAGTTGGTGAT+CGG - chr3.1:62085400-62085419 MS.gene052619:CDS 25.0%
AACACTTTACCACATGTAAT+AGG - chr3.1:62085616-62085635 MS.gene052619:intron 30.0%
AACGGTAACGTTAAAATATG+TGG - chr3.1:62084761-62084780 MS.gene052619:CDS 30.0%
ACACACTTTAAGTAGAGATT+TGG + chr3.1:62085210-62085229 None:intergenic 30.0%
AGAAGAGAGGAAATAAACAT+AGG - chr3.1:62085436-62085455 MS.gene052619:intron 30.0%
AGGGAGGATATGAAAAATTA+TGG - chr3.1:62085051-62085070 MS.gene052619:intron 30.0%
AGTGTAGATGAAATGTACAA+AGG + chr3.1:62084810-62084829 None:intergenic 30.0%
ATAGTCCCAATCAATTCTTA+GGG + chr3.1:62085020-62085039 None:intergenic 30.0%
ATCATATGATGTGGAGATTA+CGG - chr3.1:62085542-62085561 MS.gene052619:intron 30.0%
CACTTGACAAAAAAAGACAA+TGG - chr3.1:62084991-62085010 MS.gene052619:CDS 30.0%
GAAGAGAGGAAATAAACATA+GGG - chr3.1:62085437-62085456 MS.gene052619:intron 30.0%
TAATTTCACTATTACTGACC+TGG + chr3.1:62084834-62084853 None:intergenic 30.0%
!! TCTTTAATAAGTTGGTGATC+GGG - chr3.1:62085401-62085420 MS.gene052619:CDS 30.0%
!!! ACATATTTTAACGTTACCGT+TGG + chr3.1:62084762-62084781 None:intergenic 30.0%
!!! CATTGTCTTTTTTTGTCAAG+TGG + chr3.1:62084993-62085012 None:intergenic 30.0%
!!! GTTACCATTTCTTTTAGAAC+TGG + chr3.1:62084737-62084756 None:intergenic 30.0%
!!! TACCATTTCTTTTAGAACTG+GGG + chr3.1:62084735-62084754 None:intergenic 30.0%
AAGTGAAGCTGTACAATGTT+TGG - chr3.1:62085485-62085504 MS.gene052619:intron 35.0%
AATGCACCTTTGCTTTAGTT+TGG + chr3.1:62085115-62085134 None:intergenic 35.0%
ATTCTCGTTCTTTGTTGAGA+CGG + chr3.1:62085326-62085345 None:intergenic 35.0%
CATAGTCCCAATCAATTCTT+AGG + chr3.1:62085021-62085040 None:intergenic 35.0%
CATGTGGAGAATTTAGTACA+CGG + chr3.1:62085573-62085592 None:intergenic 35.0%
CTTCACCGCTTCAAAATTAT+TGG + chr3.1:62085244-62085263 None:intergenic 35.0%
TACATTTCATCTACACTTCC+AGG - chr3.1:62084813-62084832 MS.gene052619:CDS 35.0%
TATTGCATAAACATGACCCT+TGG - chr3.1:62085283-62085302 MS.gene052619:CDS 35.0%
TCATATGATGTGGAGATTAC+GGG - chr3.1:62085543-62085562 MS.gene052619:intron 35.0%
TCTCAACAAAGAACGAGAAT+CGG - chr3.1:62085326-62085345 MS.gene052619:CDS 35.0%
TTGGAGCATATATGTACAGA+AGG - chr3.1:62085504-62085523 MS.gene052619:intron 35.0%
! AAAGAAATGGTAACGTCCAA+CGG - chr3.1:62084743-62084762 MS.gene052619:CDS 35.0%
! ATGCACCTTTGCTTTAGTTT+GGG + chr3.1:62085114-62085133 None:intergenic 35.0%
! TTTTAACCAAGATGCATGCA+AGG - chr3.1:62085707-62085726 MS.gene052619:CDS 35.0%
! TTTTTGTCAAGTGGATGAGT+GGG + chr3.1:62084984-62085003 None:intergenic 35.0%
!!! CAATAATTTTGAAGCGGTGA+AGG - chr3.1:62085242-62085261 MS.gene052619:CDS 35.0%
!!! TTTTTTGTCAAGTGGATGAG+TGG + chr3.1:62084985-62085004 None:intergenic 35.0%
AGAATTGATTGGGACTATGC+AGG - chr3.1:62085022-62085041 MS.gene052619:intron 40.0%
CAATGCCCAAACTAAAGCAA+AGG - chr3.1:62085106-62085125 MS.gene052619:intron 40.0%
CATATGATGTGGAGATTACG+GGG - chr3.1:62085544-62085563 MS.gene052619:intron 40.0%
CTTGATCCAATTCATGACCA+CGG - chr3.1:62085140-62085159 MS.gene052619:CDS 40.0%
GAGAGGAAATAAACATAGGG+AGG - chr3.1:62085440-62085459 MS.gene052619:intron 40.0%
GATGAAATGTACAAAGGCGA+TGG + chr3.1:62084804-62084823 None:intergenic 40.0%
GGTGACCCTAAGAATTGATT+GGG - chr3.1:62085012-62085031 MS.gene052619:CDS 40.0%
TGGTGACCCTAAGAATTGAT+TGG - chr3.1:62085011-62085030 MS.gene052619:CDS 40.0%
TTGATCCAATTCATGACCAC+GGG - chr3.1:62085141-62085160 MS.gene052619:CDS 40.0%
! GTAGGTGTTGTAAGTCTATG+TGG - chr3.1:62085655-62085674 MS.gene052619:CDS 40.0%
!! GTCCCCAGTTCTAAAAGAAA+TGG - chr3.1:62084730-62084749 MS.gene052619:CDS 40.0%
ATGATGTCAACTTCGAGCCA+AGG + chr3.1:62085303-62085322 None:intergenic 45.0%
CAACGTGGCATCATATGATG+TGG - chr3.1:62085533-62085552 MS.gene052619:intron 45.0%
CACATCATATGATGCCACGT+TGG + chr3.1:62085535-62085554 None:intergenic 45.0%
CTCGAGTCTCCTATTACATG+TGG + chr3.1:62085628-62085647 None:intergenic 45.0%
GTAATAGGAGACTCGAGAGA+GGG - chr3.1:62085631-62085650 MS.gene052619:CDS 45.0%
GTGAAACAGAGGAAGAAGAG+AGG - chr3.1:62085423-62085442 MS.gene052619:intron 45.0%
TCCACATGTCAAGCTTTGCA+GGG - chr3.1:62085585-62085604 MS.gene052619:intron 45.0%
TGATCCAATTCATGACCACG+GGG - chr3.1:62085142-62085161 MS.gene052619:CDS 45.0%
TGATGTCAACTTCGAGCCAA+GGG + chr3.1:62085302-62085321 None:intergenic 45.0%
TGTAATAGGAGACTCGAGAG+AGG - chr3.1:62085630-62085649 MS.gene052619:CDS 45.0%
AAGCGGTGAAGGAAACAGAG+GGG - chr3.1:62085253-62085272 MS.gene052619:CDS 50.0%
ATGATGTGGAGATTACGGGG+AGG - chr3.1:62085547-62085566 MS.gene052619:intron 50.0%
ATTCATGACCACGGGGAATG+TGG - chr3.1:62085149-62085168 MS.gene052619:CDS 50.0%
CATGCAAGGTTCCTCTTTGC+AGG - chr3.1:62085721-62085740 MS.gene052619:CDS 50.0%
CATTCCCCGTGGTCATGAAT+TGG + chr3.1:62085149-62085168 None:intergenic 50.0%
CCACATGTCAAGCTTTGCAG+GGG - chr3.1:62085586-62085605 MS.gene052619:intron 50.0%
CTCCACATGTCAAGCTTTGC+AGG - chr3.1:62085584-62085603 MS.gene052619:intron 50.0%
GAAGCGGTGAAGGAAACAGA+GGG - chr3.1:62085252-62085271 MS.gene052619:CDS 50.0%
GAGGAACCTTGCATGCATCT+TGG + chr3.1:62085716-62085735 None:intergenic 50.0%
TACAAAGGCGATGGCTGCAT+TGG + chr3.1:62084795-62084814 None:intergenic 50.0%
TACAGAAGGTTGCACCAACG+TGG - chr3.1:62085518-62085537 MS.gene052619:intron 50.0%
TGAAGCGGTGAAGGAAACAG+AGG - chr3.1:62085251-62085270 MS.gene052619:CDS 50.0%
! TTGGTGATCGGGTGAAACAG+AGG - chr3.1:62085412-62085431 MS.gene052619:intron 50.0%
CCCCTGCAAAGCTTGACATG+TGG + chr3.1:62085589-62085608 None:intergenic 55.0%
! AAGGCGATGGCTGCATTGGT+TGG + chr3.1:62084791-62084810 None:intergenic 55.0%
! AGACGTGCGAGAGTACGAGA+AGG + chr3.1:62085083-62085102 None:intergenic 55.0%
!! TGTGGTGGACGTGTTGCACT+TGG - chr3.1:62085167-62085186 MS.gene052619:CDS 55.0%
ACTATGCAGGTGCAGCAGGG+AGG - chr3.1:62085035-62085054 MS.gene052619:intron 60.0%
CATGACCACGGGGAATGTGG+TGG - chr3.1:62085152-62085171 MS.gene052619:CDS 60.0%
CGGTGAAGGAAACAGAGGGG+TGG - chr3.1:62085256-62085275 MS.gene052619:CDS 60.0%
GGGACTATGCAGGTGCAGCA+GGG - chr3.1:62085032-62085051 MS.gene052619:intron 60.0%
TGGGACTATGCAGGTGCAGC+AGG - chr3.1:62085031-62085050 MS.gene052619:intron 60.0%
! GGAGACTCGAGAGAGGGTGT+AGG - chr3.1:62085637-62085656 MS.gene052619:CDS 60.0%
CACGTCCACCACATTCCCCG+TGG + chr3.1:62085160-62085179 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 62084722 62085742 62084722 ID=MS.gene052619
chr3.1 mRNA 62084722 62085742 62084722 ID=MS.gene052619.t1;Parent=MS.gene052619
chr3.1 exon 62085630 62085742 62085630 ID=MS.gene052619.t1.exon1;Parent=MS.gene052619.t1
chr3.1 CDS 62085630 62085742 62085630 ID=cds.MS.gene052619.t1;Parent=MS.gene052619.t1
chr3.1 exon 62085117 62085420 62085117 ID=MS.gene052619.t1.exon2;Parent=MS.gene052619.t1
chr3.1 CDS 62085117 62085420 62085117 ID=cds.MS.gene052619.t1;Parent=MS.gene052619.t1
chr3.1 exon 62084722 62085032 62084722 ID=MS.gene052619.t1.exon3;Parent=MS.gene052619.t1
chr3.1 CDS 62084722 62085032 62084722 ID=cds.MS.gene052619.t1;Parent=MS.gene052619.t1
Gene Sequence

>MS.gene052619

GTTTCAGCGTCCCCAGTTCTAAAAGAAATGGTAACGTCCAACGGTAACGTTAAAATATGTGGTGTTCCAACCAATGCAGCCATCGCCTTTGTACATTTCATCTACACTTCCAGGTCAGTAATAGTGAAATTAAATAACTACTCATTTTTACGTAGAAAAGAAAATTGAATTAACAAATTTTTTGGTCTAAATACATCAATTTTCGGTTACTCAATTTTCTGTCATATTTAGAACAAAGAGAGTAGTATTATACTTTTTTCCCACTCATCCACTTGACAAAAAAAGACAATGGTGACCCTAAGAATTGATTGGGACTATGCAGGTGCAGCAGGGAGGATATGAAAAATTATGGAATACACCTTCTCGTACTCTCGCACGTCTTTGCAATGCCCAAACTAAAGCAAAGGTGCATTGTTGACTTGATCCAATTCATGACCACGGGGAATGTGGTGGACGTGTTGCACTTGGCTAAGCTTTGTGATGCACCAAATCTCTACTTAAAGTGTGTCAAGTTAGTTACCAATAATTTTGAAGCGGTGAAGGAAACAGAGGGGTGGAAATTATTGCATAAACATGACCCTTGGCTCGAAGTTGACATCATCCGTCTCAACAAAGAACGAGAATCGGTAAATAATAATCATTTTATTAAAATTCAGTAATTATATTTTATTTATATATTTCTTTAATAAGTTGGTGATCGGGTGAAACAGAGGAAGAAGAGAGGAAATAAACATAGGGAGGAACAAAAGTTGTTTGTGCAATTAAGTGAAGCTGTACAATGTTTGGAGCATATATGTACAGAAGGTTGCACCAACGTGGCATCATATGATGTGGAGATTACGGGGAGGCCGTGTACTAAATTCTCCACATGTCAAGCTTTGCAGGGGTTGATCAAACACTTTACCACATGTAATAGGAGACTCGAGAGAGGGTGTAGGTGTTGTAAGTCTATGTGGCAACTACTTAGACTGCACTCTTGCATTTGTTTTAACCAAGATGCATGCAAGGTTCCTCTTTGCAG

Protein sequence

>MS.gene052619.t1

VSASPVLKEMVTSNGNVKICGVPTNAAIAFVHFIYTSRCSREDMKNYGIHLLVLSHVFAMPKLKQRCIVDLIQFMTTGNVVDVLHLAKLCDAPNLYLKCVKLVTNNFEAVKETEGWKLLHKHDPWLEVDIIRLNKERESRKKRGNKHREEQKLFVQLSEAVQCLEHICTEGCTNVASYDVEITGRPCTKFSTCQALQGLIKHFTTCNRRLERGCRCCKSMWQLLRLHSCICFNQDACKVPLC