Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052749.t1 | GAU21539.1 | 48.3 | 302 | 59 | 4 | 25 | 229 | 45 | 346 | 6.70E-57 | 230.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052749.t1 | A0A2Z6MRF5 | 48.3 | 302 | 59 | 4 | 25 | 229 | 45 | 346 | 4.9e-57 | 230.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene052749.t1 | TR | ARID |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052749.t1 | MTR_5g095070 | 50.661 | 227 | 51 | 4 | 22 | 189 | 45 | 269 | 4.10e-61 | 192 |
| MS.gene052749.t1 | MTR_1g084150 | 57.862 | 159 | 30 | 2 | 108 | 229 | 64 | 222 | 1.29e-56 | 180 |
| MS.gene052749.t1 | MTR_5g080030 | 45.327 | 214 | 60 | 4 | 28 | 184 | 63 | 276 | 4.55e-47 | 160 |
| MS.gene052749.t1 | MTR_5g080030 | 52.482 | 141 | 51 | 6 | 99 | 229 | 267 | 401 | 7.74e-30 | 115 |
| MS.gene052749.t1 | MTR_4g015240 | 81.159 | 69 | 9 | 1 | 165 | 229 | 32 | 100 | 8.72e-31 | 109 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCCTTCTTCCCAGTTGATT+TGG | 0.267970 | 4.4:+85451651 | MS.gene052749:CDS |
| ACAAAAGAGTAATTTGTTAG+AGG | 0.335421 | 4.4:-85451574 | None:intergenic |
| TTTGAGAAATTGTACTTCTC+TGG | 0.365424 | 4.4:+85451045 | MS.gene052749:CDS |
| CAGTAAGTATCCAAATCAAC+TGG | 0.365811 | 4.4:-85451661 | None:intergenic |
| AACGTTACCATAGGTGAGTT+TGG | 0.366922 | 4.4:-85450284 | None:intergenic |
| GTAAAGCAGATACAAGTCTA+AGG | 0.401467 | 4.4:-85450741 | None:intergenic |
| AGGTTGCTCTACAAAACAAA+TGG | 0.410065 | 4.4:+85450932 | MS.gene052749:intron |
| GATGTCTGTCGAGGATAAAC+AGG | 0.416822 | 4.4:+85451353 | MS.gene052749:CDS |
| AAACGGCGAGGTGGTCCAAC+TGG | 0.434685 | 4.4:+85451219 | MS.gene052749:CDS |
| GCTCATGTTCCGGACATAGA+TGG | 0.437103 | 4.4:+85451291 | MS.gene052749:CDS |
| CATAAGAGGCCTCGTGTGTC+CGG | 0.439430 | 4.4:+85451538 | MS.gene052749:CDS |
| CTGGTGAAAACCTCCTCTTC+TGG | 0.440306 | 4.4:-85450774 | None:intergenic |
| GTCTCTGCACTGAAACTAGA+AGG | 0.443281 | 4.4:+85450964 | MS.gene052749:CDS |
| ATCCAAATCAACTGGGAAGA+AGG | 0.454997 | 4.4:-85451653 | None:intergenic |
| ATAATGACACTAAACATAAG+AGG | 0.467014 | 4.4:+85451524 | MS.gene052749:CDS |
| AGACTTGTATCTGCTTTACT+TGG | 0.470775 | 4.4:+85450745 | MS.gene052749:CDS |
| TCGATTAAAAGCTCATGTTC+CGG | 0.480755 | 4.4:+85451281 | MS.gene052749:CDS |
| ATGGTAACGTTGTGTCCAAT+GGG | 0.501758 | 4.4:+85450296 | MS.gene052749:CDS |
| TGTATAAAAGTATTGCATAG+AGG | 0.502760 | 4.4:-85451020 | None:intergenic |
| TATGGTAACGTTGTGTCCAA+TGG | 0.505318 | 4.4:+85450295 | MS.gene052749:CDS |
| ATCGAGCACATTCCTGCTTG+AGG | 0.522606 | 4.4:-85451263 | None:intergenic |
| TTCCTAGCTCAAACAATGGA+AGG | 0.526147 | 4.4:+85450223 | None:intergenic |
| TCCAAATCAACTGGGAAGAA+GGG | 0.526508 | 4.4:-85451652 | None:intergenic |
| TACGAATGGCTATTGACGCT+TGG | 0.530137 | 4.4:+85451325 | MS.gene052749:CDS |
| CATTTCAAAAGCCACGTTCG+CGG | 0.533360 | 4.4:-85451608 | None:intergenic |
| ACAAGTCTAAGGATGTTTCT+CGG | 0.546217 | 4.4:-85450730 | None:intergenic |
| TACTTGGAAGTCACCAGAAG+AGG | 0.547711 | 4.4:+85450761 | MS.gene052749:CDS |
| TCAGATGCCAAACTCACCTA+TGG | 0.549356 | 4.4:+85450277 | MS.gene052749:CDS |
| GGTTGCTCTACAAAACAAAT+GGG | 0.552001 | 4.4:+85450933 | MS.gene052749:intron |
| ATGGCAAAGAAAAGAAGAAA+CGG | 0.560257 | 4.4:+85451202 | MS.gene052749:CDS |
| TACTTCTCTGGCATCCCCAA+AGG | 0.580148 | 4.4:+85451057 | MS.gene052749:CDS |
| ATATCAAATATTCCTCAAGC+AGG | 0.584597 | 4.4:+85451251 | MS.gene052749:CDS |
| TTGGAAGTCACCAGAAGAGG+AGG | 0.590853 | 4.4:+85450764 | MS.gene052749:CDS |
| GTAATTTGTTAGAGGTTGCG+AGG | 0.601369 | 4.4:-85451566 | None:intergenic |
| TAGAGGTTGCGAGGTTACAC+CGG | 0.616084 | 4.4:-85451557 | None:intergenic |
| TTTCATGTAGCCATATGTGG+AGG | 0.619171 | 4.4:+85451459 | MS.gene052749:intron |
| ATTGGACACAACGTTACCAT+AGG | 0.622181 | 4.4:-85450293 | None:intergenic |
| CAACAACGCAGCCGCGAACG+TGG | 0.625266 | 4.4:+85451597 | MS.gene052749:CDS |
| AAGCGTCAATAGCCATTCGT+AGG | 0.626242 | 4.4:-85451323 | None:intergenic |
| AAAGATGAAGGAAACAAACA+AGG | 0.649227 | 4.4:+85451489 | MS.gene052749:CDS |
| GTTCTCTTAAGATACCTTTG+GGG | 0.655260 | 4.4:-85451071 | None:intergenic |
| GAAAAGAAGAAACGGCGAGG+TGG | 0.658094 | 4.4:+85451210 | MS.gene052749:CDS |
| AGTAAGTATCCAAATCAACT+GGG | 0.664960 | 4.4:-85451660 | None:intergenic |
| TTGGCAGAAGATGTCTGTCG+AGG | 0.670700 | 4.4:+85451344 | MS.gene052749:CDS |
| GAGGTTACACCGGACACACG+AGG | 0.696311 | 4.4:-85451547 | None:intergenic |
| AAAGAAAAGAAGAAACGGCG+AGG | 0.733773 | 4.4:+85451207 | MS.gene052749:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATAGTCTAATTTTT+TGG | + | chr4.4:85450517-85450536 | MS.gene052749:intron | 10.0% |
| !! | AAAAAGCAATTAAAAATGGA+AGG | - | chr4.4:85451442-85451461 | None:intergenic | 20.0% |
| !! | ACCAATTTAATCAAGAAATT+AGG | + | chr4.4:85450836-85450855 | MS.gene052749:intron | 20.0% |
| !! | CATGAAAAAGCAATTAAAAA+TGG | - | chr4.4:85451446-85451465 | None:intergenic | 20.0% |
| ! | ATAATGACACTAAACATAAG+AGG | + | chr4.4:85451524-85451543 | MS.gene052749:CDS | 25.0% |
| ! | GCCTAATTTCTTGATTAAAT+TGG | - | chr4.4:85450840-85450859 | None:intergenic | 25.0% |
| ! | TATCTTAAGAGAACAATTTG+TGG | + | chr4.4:85451080-85451099 | MS.gene052749:intron | 25.0% |
| ! | TTAATTTACAACTCATGATC+CGG | + | chr4.4:85451173-85451192 | MS.gene052749:intron | 25.0% |
| !!! | ACAAAAGAGTAATTTGTTAG+AGG | - | chr4.4:85451577-85451596 | None:intergenic | 25.0% |
| !!! | TGTATAAAAGTATTGCATAG+AGG | - | chr4.4:85451023-85451042 | None:intergenic | 25.0% |
| AAAGATGAAGGAAACAAACA+AGG | + | chr4.4:85451489-85451508 | MS.gene052749:CDS | 30.0% | |
| AGTAAGTATCCAAATCAACT+GGG | - | chr4.4:85451663-85451682 | None:intergenic | 30.0% | |
| ATATCAAATATTCCTCAAGC+AGG | + | chr4.4:85451251-85451270 | MS.gene052749:CDS | 30.0% | |
| ATGGCAAAGAAAAGAAGAAA+CGG | + | chr4.4:85451202-85451221 | MS.gene052749:CDS | 30.0% | |
| CAATTCTCTTGAATGTTTCA+AGG | + | chr4.4:85450912-85450931 | MS.gene052749:intron | 30.0% | |
| GCATGCATTAAAAATCATCA+AGG | + | chr4.4:85450542-85450561 | MS.gene052749:intron | 30.0% | |
| TGTTCTCTTAAGATACCTTT+GGG | - | chr4.4:85451075-85451094 | None:intergenic | 30.0% | |
| TTGCATGTTTGTTTATAGAG+AGG | + | chr4.4:85450421-85450440 | MS.gene052749:intron | 30.0% | |
| TTGTTCTCTTAAGATACCTT+TGG | - | chr4.4:85451076-85451095 | None:intergenic | 30.0% | |
| TTTGAGAAATTGTACTTCTC+TGG | + | chr4.4:85451045-85451064 | MS.gene052749:CDS | 30.0% | |
| ACAAGTCTAAGGATGTTTCT+CGG | - | chr4.4:85450733-85450752 | None:intergenic | 35.0% | |
| AGACTTGTATCTGCTTTACT+TGG | + | chr4.4:85450745-85450764 | MS.gene052749:CDS | 35.0% | |
| AGGTTGCTCTACAAAACAAA+TGG | + | chr4.4:85450932-85450951 | MS.gene052749:intron | 35.0% | |
| CAGTAAGTATCCAAATCAAC+TGG | - | chr4.4:85451664-85451683 | None:intergenic | 35.0% | |
| CCTAAAATCGTACAAATACC+TGG | - | chr4.4:85450796-85450815 | None:intergenic | 35.0% | |
| CTTGCATGCATTCAAAATCA+AGG | + | chr4.4:85450457-85450476 | MS.gene052749:intron | 35.0% | |
| GGTTGCTCTACAAAACAAAT+GGG | + | chr4.4:85450933-85450952 | MS.gene052749:intron | 35.0% | |
| GTAAAGCAGATACAAGTCTA+AGG | - | chr4.4:85450744-85450763 | None:intergenic | 35.0% | |
| GTTCTCTTAAGATACCTTTG+GGG | - | chr4.4:85451074-85451093 | None:intergenic | 35.0% | |
| GTTGTTGAGTTTAACATGCA+GGG | + | chr4.4:85450610-85450629 | MS.gene052749:intron | 35.0% | |
| TCGATTAAAAGCTCATGTTC+CGG | + | chr4.4:85451281-85451300 | MS.gene052749:CDS | 35.0% | |
| TGTTGTTGAGTTTAACATGC+AGG | + | chr4.4:85450609-85450628 | MS.gene052749:intron | 35.0% | |
| ! | ACATTTTTGCCATCTATGTC+CGG | - | chr4.4:85451303-85451322 | None:intergenic | 35.0% |
| ! | CCAGGTATTTGTACGATTTT+AGG | + | chr4.4:85450793-85450812 | MS.gene052749:intron | 35.0% |
| ! | TTTTCTTTGCCATCTATGTC+CGG | - | chr4.4:85451195-85451214 | None:intergenic | 35.0% |
| ! | TTTTTGCTTTCCTCCACATA+TGG | - | chr4.4:85451472-85451491 | None:intergenic | 35.0% |
| !! | CTTTTTCATGTAGCCATATG+TGG | + | chr4.4:85451456-85451475 | MS.gene052749:intron | 35.0% |
| !!! | TGATATCCACTTTTTCCAGT+TGG | - | chr4.4:85451237-85451256 | None:intergenic | 35.0% |
| AAAGAAAAGAAGAAACGGCG+AGG | + | chr4.4:85451207-85451226 | MS.gene052749:CDS | 40.0% | |
| AACGTTACCATAGGTGAGTT+TGG | - | chr4.4:85450287-85450306 | None:intergenic | 40.0% | |
| ATCCAAATCAACTGGGAAGA+AGG | - | chr4.4:85451656-85451675 | None:intergenic | 40.0% | |
| ATTGGACACAACGTTACCAT+AGG | - | chr4.4:85450296-85450315 | None:intergenic | 40.0% | |
| CTTGAGTGAGTTTGACATGA+AGG | - | chr4.4:85450329-85450348 | None:intergenic | 40.0% | |
| GGAGGAAAGCAAAAAGATGA+AGG | + | chr4.4:85451477-85451496 | MS.gene052749:CDS | 40.0% | |
| TCCAAATCAACTGGGAAGAA+GGG | - | chr4.4:85451655-85451674 | None:intergenic | 40.0% | |
| TGGCAAAAATGTCCTACGAA+TGG | + | chr4.4:85451311-85451330 | MS.gene052749:CDS | 40.0% | |
| TGTCAAACTCACTCAAGTGT+TGG | + | chr4.4:85450332-85450351 | MS.gene052749:intron | 40.0% | |
| TTTCATGTAGCCATATGTGG+AGG | + | chr4.4:85451459-85451478 | MS.gene052749:intron | 40.0% | |
| ! | ATGGTAACGTTGTGTCCAAT+GGG | + | chr4.4:85450296-85450315 | MS.gene052749:CDS | 40.0% |
| ! | TATGGTAACGTTGTGTCCAA+TGG | + | chr4.4:85450295-85450314 | MS.gene052749:CDS | 40.0% |
| !! | GTAATTTGTTAGAGGTTGCG+AGG | - | chr4.4:85451569-85451588 | None:intergenic | 40.0% |
| AAGCGTCAATAGCCATTCGT+AGG | - | chr4.4:85451326-85451345 | None:intergenic | 45.0% | |
| ACTCATGATCCGGACATAGA+TGG | + | chr4.4:85451183-85451202 | MS.gene052749:intron | 45.0% | |
| CATGAAGGTCTCTAACCCAT+TGG | - | chr4.4:85450314-85450333 | None:intergenic | 45.0% | |
| CATTTCAAAAGCCACGTTCG+CGG | - | chr4.4:85451611-85451630 | None:intergenic | 45.0% | |
| GATGTCTGTCGAGGATAAAC+AGG | + | chr4.4:85451353-85451372 | MS.gene052749:CDS | 45.0% | |
| GTCTCTGCACTGAAACTAGA+AGG | + | chr4.4:85450964-85450983 | MS.gene052749:CDS | 45.0% | |
| TACGAATGGCTATTGACGCT+TGG | + | chr4.4:85451325-85451344 | MS.gene052749:CDS | 45.0% | |
| TACTTGGAAGTCACCAGAAG+AGG | + | chr4.4:85450761-85450780 | MS.gene052749:CDS | 45.0% | |
| TCAGATGCCAAACTCACCTA+TGG | + | chr4.4:85450277-85450296 | MS.gene052749:CDS | 45.0% | |
| TCCCTTCTTCCCAGTTGATT+TGG | + | chr4.4:85451651-85451670 | MS.gene052749:CDS | 45.0% | |
| TGACATGAGATTGAACCCAG+TGG | - | chr4.4:85450361-85450380 | None:intergenic | 45.0% | |
| !! | ACTCAAGTGTTGGATTCCAC+TGG | + | chr4.4:85450342-85450361 | MS.gene052749:intron | 45.0% |
| !! | CTCAAGTGTTGGATTCCACT+GGG | + | chr4.4:85450343-85450362 | MS.gene052749:intron | 45.0% |
| !!! | TTTTTTATTTTGAAATTATT+TGG | + | chr4.4:85450660-85450679 | MS.gene052749:intron | 5.0% |
| ATCGAGCACATTCCTGCTTG+AGG | - | chr4.4:85451266-85451285 | None:intergenic | 50.0% | |
| CTGGTGAAAACCTCCTCTTC+TGG | - | chr4.4:85450777-85450796 | None:intergenic | 50.0% | |
| GAAAAGAAGAAACGGCGAGG+TGG | + | chr4.4:85451210-85451229 | MS.gene052749:CDS | 50.0% | |
| GCTCATGTTCCGGACATAGA+TGG | + | chr4.4:85451291-85451310 | MS.gene052749:CDS | 50.0% | |
| GGTGGTCCAACTGGAAAAAG+TGG | + | chr4.4:85451228-85451247 | MS.gene052749:CDS | 50.0% | |
| TACTTCTCTGGCATCCCCAA+AGG | + | chr4.4:85451057-85451076 | MS.gene052749:CDS | 50.0% | |
| TAGAGGTTGCGAGGTTACAC+CGG | - | chr4.4:85451560-85451579 | None:intergenic | 50.0% | |
| TTGGAAGTCACCAGAAGAGG+AGG | + | chr4.4:85450764-85450783 | MS.gene052749:CDS | 50.0% | |
| TTGGCAGAAGATGTCTGTCG+AGG | + | chr4.4:85451344-85451363 | MS.gene052749:CDS | 50.0% | |
| ! | CAGAAGAGGAGGTTTTCACC+AGG | + | chr4.4:85450775-85450794 | MS.gene052749:CDS | 50.0% |
| CATAAGAGGCCTCGTGTGTC+CGG | + | chr4.4:85451538-85451557 | MS.gene052749:CDS | 55.0% | |
| AAACGGCGAGGTGGTCCAAC+TGG | + | chr4.4:85451219-85451238 | MS.gene052749:CDS | 60.0% | |
| GAGGTTACACCGGACACACG+AGG | - | chr4.4:85451550-85451569 | None:intergenic | 60.0% | |
| CAACAACGCAGCCGCGAACG+TGG | + | chr4.4:85451597-85451616 | MS.gene052749:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 85450238 | 85451684 | 85450238 | ID=MS.gene052749 |
| chr4.4 | mRNA | 85450238 | 85451684 | 85450238 | ID=MS.gene052749.t1;Parent=MS.gene052749 |
| chr4.4 | exon | 85450238 | 85450317 | 85450238 | ID=MS.gene052749.t1.exon1;Parent=MS.gene052749.t1 |
| chr4.4 | CDS | 85450238 | 85450317 | 85450238 | ID=cds.MS.gene052749.t1;Parent=MS.gene052749.t1 |
| chr4.4 | exon | 85450721 | 85450796 | 85450721 | ID=MS.gene052749.t1.exon2;Parent=MS.gene052749.t1 |
| chr4.4 | CDS | 85450721 | 85450796 | 85450721 | ID=cds.MS.gene052749.t1;Parent=MS.gene052749.t1 |
| chr4.4 | exon | 85450934 | 85451078 | 85450934 | ID=MS.gene052749.t1.exon3;Parent=MS.gene052749.t1 |
| chr4.4 | CDS | 85450934 | 85451078 | 85450934 | ID=cds.MS.gene052749.t1;Parent=MS.gene052749.t1 |
| chr4.4 | exon | 85451202 | 85451374 | 85451202 | ID=MS.gene052749.t1.exon4;Parent=MS.gene052749.t1 |
| chr4.4 | CDS | 85451202 | 85451374 | 85451202 | ID=cds.MS.gene052749.t1;Parent=MS.gene052749.t1 |
| chr4.4 | exon | 85451469 | 85451684 | 85451469 | ID=MS.gene052749.t1.exon5;Parent=MS.gene052749.t1 |
| chr4.4 | CDS | 85451469 | 85451684 | 85451469 | ID=cds.MS.gene052749.t1;Parent=MS.gene052749.t1 |
| Gene Sequence |
| Protein sequence |
