Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052835.t1 | RHN78227.1 | 75.6 | 308 | 31 | 3 | 1 | 269 | 1 | 303 | 5.80E-116 | 427.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052835.t1 | A0A396JU70 | 75.6 | 308 | 31 | 3 | 1 | 269 | 1 | 303 | 4.2e-116 | 427.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene052835.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene052835.t1 | MTR_4g119990 | 54.434 | 327 | 86 | 7 | 1 | 268 | 1 | 323 | 4.73e-105 | 310 |
| MS.gene052835.t1 | MTR_6g043890 | 51.645 | 304 | 84 | 6 | 1 | 248 | 1 | 297 | 8.43e-90 | 270 |
| MS.gene052835.t1 | MTR_1g035440 | 44.444 | 243 | 58 | 4 | 1 | 166 | 1 | 243 | 7.47e-40 | 139 |
| MS.gene052835.t1 | MTR_7g050560 | 25.279 | 269 | 159 | 6 | 13 | 242 | 11 | 276 | 1.16e-16 | 78.6 |
| MS.gene052835.t1 | MTR_1g035480 | 45.902 | 61 | 31 | 1 | 1 | 59 | 1 | 61 | 1.18e-12 | 65.1 |
| MS.gene052835.t1 | MTR_1g033740 | 41.791 | 67 | 32 | 1 | 1 | 60 | 1 | 67 | 6.51e-12 | 63.5 |
| MS.gene052835.t1 | MTR_3g098790 | 23.630 | 292 | 171 | 10 | 13 | 256 | 11 | 298 | 1.59e-11 | 63.9 |
| MS.gene052835.t1 | MTR_7g050580 | 24.535 | 269 | 161 | 7 | 13 | 242 | 11 | 276 | 1.60e-11 | 63.9 |
| MS.gene052835.t1 | MTR_1g108780 | 22.709 | 251 | 163 | 8 | 35 | 261 | 51 | 294 | 1.98e-11 | 64.3 |
| MS.gene052835.t1 | MTR_1g035460 | 26.638 | 229 | 127 | 8 | 68 | 265 | 77 | 295 | 4.46e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTATCTTTGTCAACAAAA+AGG | 0.196489 | 1.4:-20496823 | None:intergenic |
| GAAGAGAATAATTTGAAATT+TGG | 0.198679 | 1.4:+20496908 | MS.gene052835:CDS |
| CTCTATCAATGTATGACTTT+TGG | 0.220204 | 1.4:-20496432 | None:intergenic |
| CTTCATCATCACGGGTTAAA+AGG | 0.256061 | 1.4:+20496120 | MS.gene052835:CDS |
| TTTGGAAACCTTAGAGTAAC+TGG | 0.256715 | 1.4:-20495850 | None:intergenic |
| AAGTAGTTGTGATGATATTA+AGG | 0.299219 | 1.4:+20496336 | MS.gene052835:CDS |
| TTCTTACAAACTTCCATAGA+TGG | 0.339692 | 1.4:-20496158 | None:intergenic |
| ATATAATTACCTTCAAAATA+AGG | 0.343429 | 1.4:-20497019 | None:intergenic |
| TAACAAACCCAGTTACTCTA+AGG | 0.363012 | 1.4:+20495842 | MS.gene052835:CDS |
| ATTTCAAATTATTCTCTTCA+CGG | 0.368199 | 1.4:-20496904 | None:intergenic |
| GAGAAAATAACAACTTGAAA+TGG | 0.395512 | 1.4:-20496974 | None:intergenic |
| TTTCTCTTTGGAAAAGGACT+TGG | 0.399841 | 1.4:+20496991 | MS.gene052835:CDS |
| TGTTTCTTGACAAATTCATT+TGG | 0.414955 | 1.4:-20496767 | None:intergenic |
| CTTCCCTAGTTTCTCTTGAC+GGG | 0.422598 | 1.4:-20496055 | None:intergenic |
| TCTTCCCTAGTTTCTCTTGA+CGG | 0.433773 | 1.4:-20496056 | None:intergenic |
| TGAATTTGTCAAGAAACATA+TGG | 0.468376 | 1.4:+20496772 | MS.gene052835:CDS |
| TCTAAGGTTTCCAAACATGA+TGG | 0.492653 | 1.4:+20495858 | MS.gene052835:CDS |
| TGAGAACCCTTCATCATCAC+GGG | 0.503349 | 1.4:+20496112 | MS.gene052835:CDS |
| CAAGAAACATATGGACATGA+TGG | 0.508763 | 1.4:+20496781 | MS.gene052835:CDS |
| AATACCCGTCAAGAGAAACT+AGG | 0.533719 | 1.4:+20496051 | MS.gene052835:CDS |
| ATACATTGATAGAGATCGCT+TGG | 0.539899 | 1.4:+20496441 | MS.gene052835:CDS |
| TTTGTGAAAAGGTATTGGAA+AGG | 0.553882 | 1.4:+20495817 | MS.gene052835:CDS |
| AATCTGCACTAACTTGTGGT+TGG | 0.573287 | 1.4:+20496873 | MS.gene052835:CDS |
| TTGAGAACCCTTCATCATCA+CGG | 0.575621 | 1.4:+20496111 | MS.gene052835:CDS |
| ATACCCGTCAAGAGAAACTA+GGG | 0.582374 | 1.4:+20496052 | MS.gene052835:CDS |
| AAACAATCTGCACTAACTTG+TGG | 0.589005 | 1.4:+20496869 | MS.gene052835:CDS |
| GAACATGGTCATCAGAGTAA+TGG | 0.594006 | 1.4:+20495325 | MS.gene052835:CDS |
| AAACATATGGACATGATGGA+AGG | 0.599445 | 1.4:+20496785 | MS.gene052835:CDS |
| ACCATGTTCTTGAGAAGACA+TGG | 0.602247 | 1.4:-20495311 | None:intergenic |
| TTGGAAACCTTAGAGTAACT+GGG | 0.606122 | 1.4:-20495849 | None:intergenic |
| ACCATGTCTTCTCAAGAACA+TGG | 0.657917 | 1.4:+20495310 | None:intergenic |
| AAGCGTGAGCATGTGAACAA+TGG | 0.705898 | 1.4:+20496215 | MS.gene052835:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAATTACCTTCAAAATA+AGG | - | chr1.4:20497022-20497041 | None:intergenic | 15.0% |
| !!! | TTTTTGCTACTAATTTTTTT+TGG | + | chr1.4:20496290-20496309 | MS.gene052835:intron | 15.0% |
| !! | AAAATGTGATCTTATGATAT+TGG | + | chr1.4:20495594-20495613 | MS.gene052835:intron | 20.0% |
| !! | AACTATATGTGTGTATTTAT+AGG | + | chr1.4:20496739-20496758 | MS.gene052835:intron | 20.0% |
| !! | AATAGTGATTGTTCAAATAT+AGG | + | chr1.4:20495638-20495657 | MS.gene052835:intron | 20.0% |
| !! | AGAAAAACAATTTCTTCATT+AGG | - | chr1.4:20495547-20495566 | None:intergenic | 20.0% |
| !! | ATTTATCTTTGTCAACAAAA+AGG | - | chr1.4:20496826-20496845 | None:intergenic | 20.0% |
| !! | ATTTCAAATTATTCTCTTCA+CGG | - | chr1.4:20496907-20496926 | None:intergenic | 20.0% |
| !! | GAAAAACAATTTCTTCATTA+GGG | - | chr1.4:20495546-20495565 | None:intergenic | 20.0% |
| !! | TTTATCTTTGTCAACAAAAA+GGG | - | chr1.4:20496825-20496844 | None:intergenic | 20.0% |
| !!! | AATGAAGTTATATTTTGCAA+TGG | + | chr1.4:20495912-20495931 | MS.gene052835:intron | 20.0% |
| !!! | GAAGAGAATAATTTGAAATT+TGG | + | chr1.4:20496908-20496927 | MS.gene052835:CDS | 20.0% |
| !!! | TGAATTCTTATTTTTTTAGC+AGG | + | chr1.4:20496313-20496332 | MS.gene052835:intron | 20.0% |
| !!! | TTTGAATGAATCACAATTTT+TGG | + | chr1.4:20495962-20495981 | MS.gene052835:intron | 20.0% |
| !!! | TTTTTGTTGACAAAGATAAA+TGG | + | chr1.4:20496825-20496844 | MS.gene052835:CDS | 20.0% |
| ! | AAGTAGTTGTGATGATATTA+AGG | + | chr1.4:20496336-20496355 | MS.gene052835:CDS | 25.0% |
| ! | AATATAGCAAAGTCCAATTA+TGG | - | chr1.4:20496582-20496601 | None:intergenic | 25.0% |
| ! | CAAATATGCATTGCAAAATT+TGG | + | chr1.4:20495443-20495462 | MS.gene052835:intron | 25.0% |
| ! | CCAAATTAGTATACCATAAT+TGG | + | chr1.4:20496566-20496585 | MS.gene052835:intron | 25.0% |
| ! | CCAATTATGGTATACTAATT+TGG | - | chr1.4:20496569-20496588 | None:intergenic | 25.0% |
| ! | GAGAAAATAACAACTTGAAA+TGG | - | chr1.4:20496977-20496996 | None:intergenic | 25.0% |
| ! | GTCTTTAATGTGATTGTATT+CGG | + | chr1.4:20496008-20496027 | MS.gene052835:intron | 25.0% |
| ! | TGAATTTGTCAAGAAACATA+TGG | + | chr1.4:20496772-20496791 | MS.gene052835:CDS | 25.0% |
| ! | TGCATATTGTGAAAAATCAA+GGG | - | chr1.4:20495425-20495444 | None:intergenic | 25.0% |
| ! | TGTTTCTTGACAAATTCATT+TGG | - | chr1.4:20496770-20496789 | None:intergenic | 25.0% |
| ! | TTGCATATTGTGAAAAATCA+AGG | - | chr1.4:20495426-20495445 | None:intergenic | 25.0% |
| !! | AAGTTATATTTTGCAATGGT+TGG | + | chr1.4:20495916-20495935 | MS.gene052835:intron | 25.0% |
| !! | ATACCTTTTCACAAAAGTTA+TGG | - | chr1.4:20495812-20495831 | None:intergenic | 25.0% |
| !! | TACCTTTTCACAAAAGTTAT+GGG | - | chr1.4:20495811-20495830 | None:intergenic | 25.0% |
| !! | TGATGGAAAAGAAAGTTTTT+GGG | + | chr1.4:20495875-20495894 | MS.gene052835:CDS | 25.0% |
| !!! | AAGCATTTGTTATATGAAAG+TGG | - | chr1.4:20495702-20495721 | None:intergenic | 25.0% |
| !!! | AGCATTTGTTATATGAAAGT+GGG | - | chr1.4:20495701-20495720 | None:intergenic | 25.0% |
| !!! | ATGATGGAAAAGAAAGTTTT+TGG | + | chr1.4:20495874-20495893 | MS.gene052835:CDS | 25.0% |
| !!! | CTTTAAAAGTACCTACATAA+TGG | + | chr1.4:20496615-20496634 | MS.gene052835:intron | 25.0% |
| !!! | TAACTTTTGTGAAAAGGTAT+TGG | + | chr1.4:20495812-20495831 | MS.gene052835:CDS | 25.0% |
| !!! | TCAAGTTGTTATTTTCTCTT+TGG | + | chr1.4:20496979-20496998 | MS.gene052835:CDS | 25.0% |
| !!! | TGTTATTTTCTCTTTGGAAA+AGG | + | chr1.4:20496985-20497004 | MS.gene052835:CDS | 25.0% |
| AAGAAACACAACATGATGAT+AGG | - | chr1.4:20496251-20496270 | None:intergenic | 30.0% | |
| AGTGATTGTTCAAATATAGG+TGG | + | chr1.4:20495641-20495660 | MS.gene052835:intron | 30.0% | |
| GAAAAAATTCGACTCTGATA+AGG | + | chr1.4:20496390-20496409 | MS.gene052835:CDS | 30.0% | |
| GACGAATAACCTTAAAAAAG+TGG | - | chr1.4:20495354-20495373 | None:intergenic | 30.0% | |
| TTCTTACAAACTTCCATAGA+TGG | - | chr1.4:20496161-20496180 | None:intergenic | 30.0% | |
| ! | AAAATTTTCAGCACCATCTA+TGG | + | chr1.4:20496145-20496164 | MS.gene052835:CDS | 30.0% |
| ! | ACTTTCTTTTCCATCATGTT+TGG | - | chr1.4:20495871-20495890 | None:intergenic | 30.0% |
| ! | CTCTATCAATGTATGACTTT+TGG | - | chr1.4:20496435-20496454 | None:intergenic | 30.0% |
| ! | TACCAGTTTTTCTTCTTGAA+GGG | - | chr1.4:20495383-20495402 | None:intergenic | 30.0% |
| ! | TTACCAGTTTTTCTTCTTGA+AGG | - | chr1.4:20495384-20495403 | None:intergenic | 30.0% |
| !! | TGTGTTTTTGTGCTAAACAA+AGG | + | chr1.4:20496938-20496957 | MS.gene052835:CDS | 30.0% |
| !! | TTTGTGAAAAGGTATTGGAA+AGG | + | chr1.4:20495817-20495836 | MS.gene052835:CDS | 30.0% |
| !!! | CGAATTTTTTCTTCACTTTC+TGG | - | chr1.4:20496381-20496400 | None:intergenic | 30.0% |
| !!! | TAATGGTGTCCACTTTTTTA+AGG | + | chr1.4:20495342-20495361 | MS.gene052835:CDS | 30.0% |
| !!! | TTGGAAACTCCTTATTTTGA+AGG | + | chr1.4:20497010-20497029 | MS.gene052835:CDS | 30.0% |
| AAACAATCTGCACTAACTTG+TGG | + | chr1.4:20496869-20496888 | MS.gene052835:CDS | 35.0% | |
| ATACATTGATAGAGATCGCT+TGG | + | chr1.4:20496441-20496460 | MS.gene052835:CDS | 35.0% | |
| CAAGAAACATATGGACATGA+TGG | + | chr1.4:20496781-20496800 | MS.gene052835:CDS | 35.0% | |
| CCAATCACAATCCATTATGT+AGG | - | chr1.4:20496629-20496648 | None:intergenic | 35.0% | |
| TAACAAACCCAGTTACTCTA+AGG | + | chr1.4:20495842-20495861 | MS.gene052835:CDS | 35.0% | |
| TACCCTTCAAGAAGAAAAAC+TGG | + | chr1.4:20495378-20495397 | MS.gene052835:CDS | 35.0% | |
| TTGGAAACCTTAGAGTAACT+GGG | - | chr1.4:20495852-20495871 | None:intergenic | 35.0% | |
| TTTGGAAACCTTAGAGTAAC+TGG | - | chr1.4:20495853-20495872 | None:intergenic | 35.0% | |
| ! | AAACATATGGACATGATGGA+AGG | + | chr1.4:20496785-20496804 | MS.gene052835:CDS | 35.0% |
| ! | TCTAAGGTTTCCAAACATGA+TGG | + | chr1.4:20495858-20495877 | MS.gene052835:CDS | 35.0% |
| ! | TGCCCATAACTTTTGTGAAA+AGG | + | chr1.4:20495806-20495825 | MS.gene052835:CDS | 35.0% |
| ! | TTTCTCTTTGGAAAAGGACT+TGG | + | chr1.4:20496991-20497010 | MS.gene052835:CDS | 35.0% |
| !! | CCTACATAATGGATTGTGAT+TGG | + | chr1.4:20496626-20496645 | MS.gene052835:intron | 35.0% |
| !! | TTTTAACCCGTGATGATGAA+GGG | - | chr1.4:20496121-20496140 | None:intergenic | 35.0% |
| AATACCCGTCAAGAGAAACT+AGG | + | chr1.4:20496051-20496070 | MS.gene052835:CDS | 40.0% | |
| ATACCCGTCAAGAGAAACTA+GGG | + | chr1.4:20496052-20496071 | MS.gene052835:CDS | 40.0% | |
| GAACATGGTCATCAGAGTAA+TGG | + | chr1.4:20495325-20495344 | MS.gene052835:CDS | 40.0% | |
| GTGATTGTATTCGGTAGATG+CGG | + | chr1.4:20496017-20496036 | MS.gene052835:intron | 40.0% | |
| TCTTCCCTAGTTTCTCTTGA+CGG | - | chr1.4:20496059-20496078 | None:intergenic | 40.0% | |
| TTGAGAACCCTTCATCATCA+CGG | + | chr1.4:20496111-20496130 | MS.gene052835:CDS | 40.0% | |
| ! | AATCTGCACTAACTTGTGGT+TGG | + | chr1.4:20496873-20496892 | MS.gene052835:CDS | 40.0% |
| ! | CTTCATCATCACGGGTTAAA+AGG | + | chr1.4:20496120-20496139 | MS.gene052835:CDS | 40.0% |
| !! | CTTTTAACCCGTGATGATGA+AGG | - | chr1.4:20496122-20496141 | None:intergenic | 40.0% |
| AAGCGTGAGCATGTGAACAA+TGG | + | chr1.4:20496215-20496234 | MS.gene052835:CDS | 45.0% | |
| ATTAGGGCTGCATCCAACAA+AGG | - | chr1.4:20495530-20495549 | None:intergenic | 45.0% | |
| CTTCCCTAGTTTCTCTTGAC+GGG | - | chr1.4:20496058-20496077 | None:intergenic | 45.0% | |
| GTGAGCATGTGAACAATGGT+TGG | + | chr1.4:20496219-20496238 | MS.gene052835:intron | 45.0% | |
| TGAGAACCCTTCATCATCAC+GGG | + | chr1.4:20496112-20496131 | MS.gene052835:CDS | 45.0% | |
| ! | CATCCAACAAAGGCTAGCTA+GGG | - | chr1.4:20495520-20495539 | None:intergenic | 45.0% |
| ! | GCATCCAACAAAGGCTAGCT+AGG | - | chr1.4:20495521-20495540 | None:intergenic | 50.0% |
| !! | CATCCCTAGCTAGCCTTTGT+TGG | + | chr1.4:20495514-20495533 | MS.gene052835:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 20495313 | 20497045 | 20495313 | ID=MS.gene052835 |
| chr1.4 | mRNA | 20495313 | 20497045 | 20495313 | ID=MS.gene052835.t1;Parent=MS.gene052835 |
| chr1.4 | exon | 20495313 | 20495399 | 20495313 | ID=MS.gene052835.t1.exon1;Parent=MS.gene052835.t1 |
| chr1.4 | CDS | 20495313 | 20495399 | 20495313 | ID=cds.MS.gene052835.t1;Parent=MS.gene052835.t1 |
| chr1.4 | exon | 20495802 | 20495917 | 20495802 | ID=MS.gene052835.t1.exon2;Parent=MS.gene052835.t1 |
| chr1.4 | CDS | 20495802 | 20495917 | 20495802 | ID=cds.MS.gene052835.t1;Parent=MS.gene052835.t1 |
| chr1.4 | exon | 20496034 | 20496236 | 20496034 | ID=MS.gene052835.t1.exon3;Parent=MS.gene052835.t1 |
| chr1.4 | CDS | 20496034 | 20496236 | 20496034 | ID=cds.MS.gene052835.t1;Parent=MS.gene052835.t1 |
| chr1.4 | exon | 20496335 | 20496462 | 20496335 | ID=MS.gene052835.t1.exon4;Parent=MS.gene052835.t1 |
| chr1.4 | CDS | 20496335 | 20496462 | 20496335 | ID=cds.MS.gene052835.t1;Parent=MS.gene052835.t1 |
| chr1.4 | exon | 20496761 | 20497045 | 20496761 | ID=MS.gene052835.t1.exon5;Parent=MS.gene052835.t1 |
| chr1.4 | CDS | 20496761 | 20497045 | 20496761 | ID=cds.MS.gene052835.t1;Parent=MS.gene052835.t1 |
| Gene Sequence |
| Protein sequence |
