AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053186

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053186.t1 MTR_4g007490 95.526 380 10 1 1 380 1 373 0.0 737
MS.gene053186.t1 MTR_3g450500 67.717 381 116 5 1 380 1 375 1.54e-178 501
MS.gene053186.t1 MTR_2g019840 37.714 350 204 6 32 378 34 372 1.82e-69 223
MS.gene053186.t1 MTR_2g019810 37.158 366 218 5 14 378 6 360 1.80e-68 220
MS.gene053186.t1 MTR_4g119550 34.835 333 209 5 45 375 52 378 1.30e-58 195
MS.gene053186.t1 MTR_4g120380 32.194 351 224 5 27 374 30 369 4.76e-58 193
MS.gene053186.t1 MTR_4g120380 32.530 332 210 5 27 355 30 350 5.22e-55 187
MS.gene053186.t1 MTR_2g437260 29.851 335 226 3 43 374 35 363 2.59e-53 181
MS.gene053186.t1 MTR_2g437030 33.148 359 226 7 22 375 105 454 5.10e-53 182
MS.gene053186.t1 MTR_4g119580 32.877 365 225 6 22 379 23 374 8.62e-53 179
MS.gene053186.t1 MTR_2g437040 33.141 347 223 5 32 375 28 368 3.61e-52 178
MS.gene053186.t1 MTR_2g436460 33.526 346 210 6 36 374 41 373 4.37e-52 178
MS.gene053186.t1 MTR_2g436400 30.939 362 240 6 17 375 21 375 8.38e-50 172
MS.gene053186.t1 MTR_2g437130 31.746 378 244 8 1 375 3 369 1.47e-49 171
MS.gene053186.t1 MTR_2g437080 30.811 370 245 6 11 375 5 368 8.68e-49 169
MS.gene053186.t1 MTR_2g437160 31.339 351 229 8 29 375 51 393 5.14e-48 168
MS.gene053186.t1 MTR_2g437020 32.861 353 217 9 13 356 9 350 8.68e-48 167
MS.gene053186.t1 MTR_2g437150 31.405 363 237 7 22 375 29 388 8.83e-48 167
MS.gene053186.t1 MTR_2g436440 30.423 355 235 6 24 375 29 374 9.36e-47 164
MS.gene053186.t1 MTR_2g437060 31.282 390 231 9 10 375 7 383 1.43e-46 164
MS.gene053186.t1 MTR_2g437240 29.067 375 223 5 39 375 27 396 6.54e-46 162
MS.gene053186.t1 MTR_2g437960 29.970 337 228 6 46 378 55 387 6.23e-45 160
MS.gene053186.t1 MTR_2g437960 29.970 337 228 6 46 378 55 387 9.49e-45 160
MS.gene053186.t1 MTR_2g437120 29.805 359 241 6 22 375 29 381 2.18e-44 159
MS.gene053186.t1 MTR_2g437170 30.028 363 242 7 22 375 29 388 6.39e-44 157
MS.gene053186.t1 MTR_2g437100 29.288 379 252 8 15 380 23 398 7.24e-44 157
MS.gene053186.t1 MTR_2g437180 30.422 332 223 5 46 374 51 377 3.77e-43 155
MS.gene053186.t1 MTR_2g437200 28.947 380 225 8 35 375 23 396 4.14e-43 154
MS.gene053186.t1 MTR_2g076320 30.707 368 243 8 15 374 17 380 5.87e-42 152
MS.gene053186.t1 MTR_2g438010 29.620 368 247 8 15 374 37 400 6.66e-42 152
MS.gene053186.t1 MTR_2g437990 29.973 367 246 6 15 374 17 379 8.33e-42 152
MS.gene053186.t1 MTR_2g438020 27.596 366 226 3 46 375 73 435 3.86e-39 146
MS.gene053186.t1 MTR_8g081000 24.927 341 236 3 36 375 64 385 8.22e-31 121
MS.gene053186.t1 MTR_8g081000 24.927 341 236 3 36 375 64 385 8.96e-31 121
MS.gene053186.t1 MTR_8g081000 24.927 341 236 3 36 375 64 385 9.78e-31 121
MS.gene053186.t1 MTR_8g080990 27.485 342 238 5 44 380 41 377 3.05e-29 117
MS.gene053186.t1 MTR_2g437940 26.866 335 207 7 46 375 51 352 1.31e-28 115
MS.gene053186.t1 MTR_4g119570 28.205 234 129 3 138 371 31 225 1.06e-23 98.6
MS.gene053186.t1 MTR_2g437090 29.605 152 107 0 224 375 5 156 6.01e-21 89.4
MS.gene053186.t1 MTR_2g436370 37.143 175 103 5 27 198 30 200 8.36e-21 90.1
MS.gene053186.t1 MTR_2g437160 28.767 219 135 6 177 375 85 302 1.59e-20 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053186.t1 AT5G07900 41.176 340 197 3 37 374 61 399 2.58e-88 273
MS.gene053186.t1 AT1G21150 33.693 371 239 4 10 374 17 386 2.61e-61 202
MS.gene053186.t1 AT1G21150 33.693 371 239 4 10 374 81 450 1.17e-60 202
MS.gene053186.t1 AT1G21150 34.000 350 222 4 33 374 2 350 2.38e-59 196
MS.gene053186.t1 AT1G62120 30.179 391 226 7 14 360 18 405 3.68e-43 155
MS.gene053186.t1 AT1G61980 29.412 357 210 5 41 360 48 399 9.35e-40 146
MS.gene053186.t1 AT1G61980 29.412 357 210 5 41 360 48 399 9.35e-40 146
MS.gene053186.t1 AT1G61980 29.412 357 210 5 41 360 48 399 9.35e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61970 28.650 363 207 7 41 361 48 400 9.94e-40 146
MS.gene053186.t1 AT1G61990 28.052 385 226 6 27 371 32 405 1.27e-39 145
MS.gene053186.t1 AT1G61990 28.052 385 226 6 27 371 32 405 1.27e-39 145
MS.gene053186.t1 AT3G46950 32.226 301 188 6 14 309 18 307 8.86e-38 141
MS.gene053186.t1 AT5G23930 29.120 443 222 10 7 370 12 441 5.40e-36 137
MS.gene053186.t1 AT1G62010 29.282 362 192 9 42 361 45 384 6.05e-36 135
MS.gene053186.t1 AT1G62150 31.900 279 175 5 35 307 48 317 1.29e-33 130
MS.gene053186.t1 AT1G62085 32.248 307 185 8 14 307 18 314 1.74e-32 127
MS.gene053186.t1 AT1G62085 32.248 307 185 8 14 307 18 314 1.74e-32 127
MS.gene053186.t1 AT1G61960 33.577 274 173 6 27 296 32 300 1.44e-31 124
MS.gene053186.t1 AT1G62110 31.633 294 174 7 27 307 32 311 3.62e-31 123
MS.gene053186.t1 AT5G64950 29.221 308 207 5 77 375 71 376 1.14e-30 121
MS.gene053186.t1 AT1G56380 23.161 367 231 11 39 361 10 369 2.12e-18 86.3
MS.gene053186.t1 AT1G56380 23.161 367 231 11 39 361 21 380 2.69e-18 86.3
MS.gene053186.t1 AT1G62490 27.341 267 150 7 30 296 83 305 2.64e-15 76.6

Find 68 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACCTTGTTGAAAGTTTA+AGG 0.085452 4.2:+92254166 None:intergenic
GAAAGATTTGCCATTGTTAT+TGG 0.156741 4.2:-92253260 MS.gene053186:CDS
CTTGTCACCAATCAAGATTT+TGG 0.169173 4.2:+92253960 None:intergenic
TAAGTATGGACAAAGAAATT+TGG 0.197332 4.2:-92253612 MS.gene053186:CDS
TATGAAAACAATGGACTTCT+TGG 0.234883 4.2:-92253485 MS.gene053186:CDS
GAATGCTACACAAGGAAAAT+TGG 0.258902 4.2:+92253732 None:intergenic
CCTAGTTTGCGATGATAAAA+AGG 0.296431 4.2:-92253881 MS.gene053186:CDS
TTCCTCACTTGTCATAATAA+AGG 0.304109 4.2:+92253315 None:intergenic
AAAGAACAGTAGTTGAATAA+AGG 0.305931 4.2:+92254307 None:intergenic
CATTTCTTTGACTGAATTAA+CGG 0.309788 4.2:+92253684 None:intergenic
AATGGCAAATCTTTCTGAAA+TGG 0.311445 4.2:+92253268 None:intergenic
AAAAGCTGAATAACAGCATT+TGG 0.312391 4.2:+92254123 None:intergenic
ATAACAGCATTTGGGCCATT+AGG 0.321633 4.2:+92254132 None:intergenic
ACAGCAGAGAAAAGTGAAAA+AGG 0.329234 4.2:+92254270 None:intergenic
AAGTATGGACAAAGAAATTT+GGG 0.343578 4.2:-92253611 MS.gene053186:CDS
GATTTCCCCAAAATCTTGAT+TGG 0.348520 4.2:-92253967 MS.gene053186:CDS
TCAAAGTTCTATTTCCAGTT+TGG 0.350471 4.2:-92253758 MS.gene053186:CDS
TAACAGCATTTGGGCCATTA+GGG 0.351944 4.2:+92254133 None:intergenic
GGGCATGTAGCAATGTCTTC+TGG 0.354285 4.2:+92253442 None:intergenic
TCACATACTCGGGATACTTT+AGG 0.357510 4.2:+92253527 None:intergenic
ATGGCAAATCTTTCTGAAAT+GGG 0.369413 4.2:+92253269 None:intergenic
CAACCTTGTTGAAAGTTTAA+GGG 0.375048 4.2:+92254167 None:intergenic
GTTAATTCAGTCAAAGAAAT+GGG 0.384671 4.2:-92253682 MS.gene053186:CDS
ATCTTGATAACAGCACATTT+AGG 0.385800 4.2:+92253382 None:intergenic
AGGAAAATTGGATACCAAAC+TGG 0.392789 4.2:+92253744 None:intergenic
ACAACCCACATGAGTTTATG+AGG 0.423242 4.2:+92254202 None:intergenic
AAGTGTCTTCTCCAAGTTGC+GGG 0.424163 4.2:+92253408 None:intergenic
CTCTGAGATCATCACATACT+CGG 0.424785 4.2:+92253516 None:intergenic
CGTTAATTCAGTCAAAGAAA+TGG 0.425609 4.2:-92253683 MS.gene053186:CDS
AAGAACAGTAGTTGAATAAA+GGG 0.427774 4.2:+92254308 None:intergenic
TCCAACACAAATCAACATAA+AGG 0.430035 4.2:-92254246 MS.gene053186:CDS
TAGCTTTGAGGCGTGGATCA+TGG 0.435733 4.2:-92253852 MS.gene053186:CDS
GATTCGAGTAATTGTAAGTA+TGG 0.436929 4.2:-92253626 MS.gene053186:CDS
TTCCTCAAAACCTCAATGTT+CGG 0.445259 4.2:+92253793 None:intergenic
AAAAGGTAGTTCTAGCTTTG+AGG 0.445792 4.2:-92253864 MS.gene053186:CDS
ACCTTTATGTTGATTTGTGT+TGG 0.460913 4.2:+92254245 None:intergenic
AAGTTGCGGGTCAGAATCCC+AGG 0.461038 4.2:+92253421 None:intergenic
CTCAACAAATCTAGAATGCT+TGG 0.465273 4.2:+92253708 None:intergenic
AAAGCTGAATAACAGCATTT+GGG 0.472696 4.2:+92254124 None:intergenic
TCAAGAGGTTTAATAAAGAG+TGG 0.472917 4.2:-92253352 MS.gene053186:CDS
GAAGACATTGCTACATGCCC+TGG 0.473610 4.2:-92253439 MS.gene053186:CDS
CAAAAGGGTGTTTCTTAACA+AGG 0.481935 4.2:+92254064 None:intergenic
TTGCAACTCATAAACCCTAA+TGG 0.484587 4.2:-92254147 MS.gene053186:CDS
GGACTTCTTGGTGAAGGATG+TGG 0.492155 4.2:-92253473 MS.gene053186:CDS
TATGAGGTTTGACAATGTAA+AGG 0.499828 4.2:+92254218 None:intergenic
TGGGATTCTGACCCGCAACT+TGG 0.503137 4.2:-92253419 MS.gene053186:CDS
ATCAATGGTTGAAGCCCCAA+TGG 0.538102 4.2:+92253987 None:intergenic
GACTTCTTGGTGAAGGATGT+GGG 0.555487 4.2:-92253472 MS.gene053186:CDS
CAAGCATTCTAGATTTGTTG+AGG 0.558555 4.2:-92253707 MS.gene053186:CDS
GCAATGAGAAAATGATACAT+TGG 0.568531 4.2:+92254354 None:intergenic
AAAACAAAATACAACTTCGA+AGG 0.568698 4.2:+92253652 None:intergenic
TGATTCTGTTCCGAACATTG+AGG 0.570760 4.2:-92253803 MS.gene053186:CDS
TCAAACCTCATAAACTCATG+TGG 0.578097 4.2:-92254207 MS.gene053186:CDS
TCTGAGATCATCACATACTC+GGG 0.583638 4.2:+92253517 None:intergenic
GTAGTTCTAGCTTTGAGGCG+TGG 0.585169 4.2:-92253859 MS.gene053186:CDS
AACAATGGACTTCTTGGTGA+AGG 0.587900 4.2:-92253479 MS.gene053186:CDS
AAGACATTGCTACATGCCCT+GGG 0.601471 4.2:-92253438 MS.gene053186:CDS
CAAGTGTCTTCTCCAAGTTG+CGG 0.602943 4.2:+92253407 None:intergenic
AAACTGGAAATAGAACTTTG+AGG 0.609479 4.2:+92253760 None:intergenic
TACCTTTATTATGACAAGTG+AGG 0.612836 4.2:-92253317 MS.gene053186:CDS
TGGCAAATCTTTCTGAAATG+GGG 0.627523 4.2:+92253270 None:intergenic
TTATATGCATCCAATAACAA+TGG 0.629215 4.2:+92253250 None:intergenic
CGGAACAGAATCATTAACCA+TGG 0.638798 4.2:+92253813 None:intergenic
CAAACCTCATAAACTCATGT+GGG 0.639091 4.2:-92254206 MS.gene053186:CDS
AAGCCCTTAAACTTTCAACA+AGG 0.642886 4.2:-92254170 MS.gene053186:CDS
TTAATTCAGTCAAAGAAATG+GGG 0.653020 4.2:-92253681 MS.gene053186:CDS
TGCTTGGTGAATGCTACACA+AGG 0.683625 4.2:+92253724 None:intergenic
AGTTGCGGGTCAGAATCCCA+GGG 0.702455 4.2:+92253422 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAAAAATTATGAAAACAA+TGG - chr4.2:92254088-92254107 MS.gene053186:CDS 15.0%
!!! ATTTTGATAAAAGTACAAAA+GGG + chr4.2:92253536-92253555 None:intergenic 15.0%
!!! GATTTTGATAAAAGTACAAA+AGG + chr4.2:92253537-92253556 None:intergenic 20.0%
! AAAACAAAATACAACTTCGA+AGG + chr4.2:92253933-92253952 None:intergenic 25.0%
! AAAGAACAGTAGTTGAATAA+AGG + chr4.2:92253278-92253297 None:intergenic 25.0%
! AAGAACAGTAGTTGAATAAA+GGG + chr4.2:92253277-92253296 None:intergenic 25.0%
! AAGTATGGACAAAGAAATTT+GGG - chr4.2:92253971-92253990 MS.gene053186:CDS 25.0%
! CATTTCTTTGACTGAATTAA+CGG + chr4.2:92253901-92253920 None:intergenic 25.0%
! GTTAATTCAGTCAAAGAAAT+GGG - chr4.2:92253900-92253919 MS.gene053186:CDS 25.0%
! TAAGTATGGACAAAGAAATT+TGG - chr4.2:92253970-92253989 MS.gene053186:CDS 25.0%
! TTAATTCAGTCAAAGAAATG+GGG - chr4.2:92253901-92253920 MS.gene053186:CDS 25.0%
! TTATATGCATCCAATAACAA+TGG + chr4.2:92254335-92254354 None:intergenic 25.0%
!!! CAAAATTTAAGATTTTCGAG+AGG - chr4.2:92253997-92254016 MS.gene053186:CDS 25.0%
!!! TTCGAAGTTGTATTTTGTTT+TGG - chr4.2:92253932-92253951 MS.gene053186:CDS 25.0%
AAAAGCTGAATAACAGCATT+TGG + chr4.2:92253462-92253481 None:intergenic 30.0%
AAACTGGAAATAGAACTTTG+AGG + chr4.2:92253825-92253844 None:intergenic 30.0%
AAAGCTGAATAACAGCATTT+GGG + chr4.2:92253461-92253480 None:intergenic 30.0%
CAACCTTGTTGAAAGTTTAA+GGG + chr4.2:92253418-92253437 None:intergenic 30.0%
CGTTAATTCAGTCAAAGAAA+TGG - chr4.2:92253899-92253918 MS.gene053186:CDS 30.0%
GAAAGATTTGCCATTGTTAT+TGG - chr4.2:92254322-92254341 MS.gene053186:CDS 30.0%
GATTCGAGTAATTGTAAGTA+TGG - chr4.2:92253956-92253975 MS.gene053186:CDS 30.0%
TACCTTTATTATGACAAGTG+AGG - chr4.2:92254265-92254284 MS.gene053186:CDS 30.0%
TATGAGGTTTGACAATGTAA+AGG + chr4.2:92253367-92253386 None:intergenic 30.0%
TCAAAGTTCTATTTCCAGTT+TGG - chr4.2:92253824-92253843 MS.gene053186:CDS 30.0%
TCAAGAGGTTTAATAAAGAG+TGG - chr4.2:92254230-92254249 MS.gene053186:CDS 30.0%
TCCAACACAAATCAACATAA+AGG - chr4.2:92253336-92253355 MS.gene053186:CDS 30.0%
TTCCTCACTTGTCATAATAA+AGG + chr4.2:92254270-92254289 None:intergenic 30.0%
! AATGGCAAATCTTTCTGAAA+TGG + chr4.2:92254317-92254336 None:intergenic 30.0%
! ATCTTGATAACAGCACATTT+AGG + chr4.2:92254203-92254222 None:intergenic 30.0%
! GAACTTTTACAATGATTCCA+TGG - chr4.2:92253752-92253771 MS.gene053186:CDS 30.0%
! GATTGCAAAAACTTGAGTTT+TGG + chr4.2:92253576-92253595 None:intergenic 30.0%
! TATGAAAACAATGGACTTCT+TGG - chr4.2:92254097-92254116 MS.gene053186:CDS 30.0%
! TCAAGTTTTTGCAATCCATT+GGG - chr4.2:92253580-92253599 MS.gene053186:CDS 30.0%
! TTGTCACCAATCAAGATTTT+GGG + chr4.2:92253624-92253643 None:intergenic 30.0%
!! AAAACTTGAGTTTTGGCAAA+AGG + chr4.2:92253569-92253588 None:intergenic 30.0%
!! AAACTTGAGTTTTGGCAAAA+GGG + chr4.2:92253568-92253587 None:intergenic 30.0%
!! AATTTAAGATTTTCGAGAGG+TGG - chr4.2:92254000-92254019 MS.gene053186:CDS 30.0%
!! ACCTTTATGTTGATTTGTGT+TGG + chr4.2:92253340-92253359 None:intergenic 30.0%
!! ATGGCAAATCTTTCTGAAAT+GGG + chr4.2:92254316-92254335 None:intergenic 30.0%
!! ATTTAAGATTTTCGAGAGGT+GGG - chr4.2:92254001-92254020 MS.gene053186:CDS 30.0%
!! CTTTTAAGAATCTTGTAACG+AGG + chr4.2:92253681-92253700 None:intergenic 30.0%
!!! ATCAAGATTTTGAAGTCAAG+AGG - chr4.2:92254215-92254234 MS.gene053186:CDS 30.0%
AAGCCCTTAAACTTTCAACA+AGG - chr4.2:92253412-92253431 MS.gene053186:CDS 35.0%
ACAGCAGAGAAAAGTGAAAA+AGG + chr4.2:92253315-92253334 None:intergenic 35.0%
AGGAAAATTGGATACCAAAC+TGG + chr4.2:92253841-92253860 None:intergenic 35.0%
CAAACCTCATAAACTCATGT+GGG - chr4.2:92253376-92253395 MS.gene053186:CDS 35.0%
GAATGCTACACAAGGAAAAT+TGG + chr4.2:92253853-92253872 None:intergenic 35.0%
GATTTCCCCAAAATCTTGAT+TGG - chr4.2:92253615-92253634 MS.gene053186:CDS 35.0%
GCAACCTTGTTGAAAGTTTA+AGG + chr4.2:92253419-92253438 None:intergenic 35.0%
TCAAACCTCATAAACTCATG+TGG - chr4.2:92253375-92253394 MS.gene053186:CDS 35.0%
TTCCTCAAAACCTCAATGTT+CGG + chr4.2:92253792-92253811 None:intergenic 35.0%
TTGCAACTCATAAACCCTAA+TGG - chr4.2:92253435-92253454 MS.gene053186:CDS 35.0%
! ATTCAGCTTTTCAGAAGCTA+TGG - chr4.2:92253471-92253490 MS.gene053186:CDS 35.0%
! CAAGTTTTTGCAATCCATTG+GGG - chr4.2:92253581-92253600 MS.gene053186:CDS 35.0%
! CCTAGTTTGCGATGATAAAA+AGG - chr4.2:92253701-92253720 MS.gene053186:CDS 35.0%
! CCTTTTTATCATCGCAAACT+AGG + chr4.2:92253704-92253723 None:intergenic 35.0%
! CTCAACAAATCTAGAATGCT+TGG + chr4.2:92253877-92253896 None:intergenic 35.0%
! CTCAAGTTTTTGCAATCCAT+TGG - chr4.2:92253579-92253598 MS.gene053186:CDS 35.0%
! CTTGTCACCAATCAAGATTT+TGG + chr4.2:92253625-92253644 None:intergenic 35.0%
! TGTCACCAATCAAGATTTTG+GGG + chr4.2:92253623-92253642 None:intergenic 35.0%
!! AAAAGGTAGTTCTAGCTTTG+AGG - chr4.2:92253718-92253737 MS.gene053186:CDS 35.0%
!! CAAAAGGGTGTTTCTTAACA+AGG + chr4.2:92253521-92253540 None:intergenic 35.0%
!! CAAGATTTTGGGGAAATCAA+TGG + chr4.2:92253613-92253632 None:intergenic 35.0%
!! CAAGCATTCTAGATTTGTTG+AGG - chr4.2:92253875-92253894 MS.gene053186:CDS 35.0%
!! GAAAGTTTAAGGGCTTTTTC+AGG + chr4.2:92253408-92253427 None:intergenic 35.0%
!! TGGCAAATCTTTCTGAAATG+GGG + chr4.2:92254315-92254334 None:intergenic 35.0%
!! TTTAAGATTTTCGAGAGGTG+GGG - chr4.2:92254002-92254021 MS.gene053186:CDS 35.0%
ACAACCCACATGAGTTTATG+AGG + chr4.2:92253383-92253402 None:intergenic 40.0%
ATAACAGCATTTGGGCCATT+AGG + chr4.2:92253453-92253472 None:intergenic 40.0%
CGGAACAGAATCATTAACCA+TGG + chr4.2:92253772-92253791 None:intergenic 40.0%
TAACAGCATTTGGGCCATTA+GGG + chr4.2:92253452-92253471 None:intergenic 40.0%
TCACATACTCGGGATACTTT+AGG + chr4.2:92254058-92254077 None:intergenic 40.0%
TGATTCTGTTCCGAACATTG+AGG - chr4.2:92253779-92253798 MS.gene053186:CDS 40.0%
! AACAATGGACTTCTTGGTGA+AGG - chr4.2:92254103-92254122 MS.gene053186:CDS 40.0%
! CAAGTTTTTGACAGCAAGCT+TGG - chr4.2:92253641-92253660 MS.gene053186:CDS 40.0%
! TCTGAGATCATCACATACTC+GGG + chr4.2:92254068-92254087 None:intergenic 40.0%
! TTCCGAACATTGAGGTTTTG+AGG - chr4.2:92253787-92253806 MS.gene053186:CDS 40.0%
!! CTCTGAGATCATCACATACT+CGG + chr4.2:92254069-92254088 None:intergenic 40.0%
!!! ATTGAGGTTTTGAGGAAGCT+TGG - chr4.2:92253795-92253814 MS.gene053186:CDS 40.0%
AAGACATTGCTACATGCCCT+GGG - chr4.2:92254144-92254163 MS.gene053186:CDS 45.0%
AAGTGTCTTCTCCAAGTTGC+GGG + chr4.2:92254177-92254196 None:intergenic 45.0%
ATCAATGGTTGAAGCCCCAA+TGG + chr4.2:92253598-92253617 None:intergenic 45.0%
CAAGTGTCTTCTCCAAGTTG+CGG + chr4.2:92254178-92254197 None:intergenic 45.0%
GACTTCTTGGTGAAGGATGT+GGG - chr4.2:92254110-92254129 MS.gene053186:CDS 45.0%
! TGCTTGGTGAATGCTACACA+AGG + chr4.2:92253861-92253880 None:intergenic 45.0%
GAAGACATTGCTACATGCCC+TGG - chr4.2:92254143-92254162 MS.gene053186:CDS 50.0%
GGACTTCTTGGTGAAGGATG+TGG - chr4.2:92254109-92254128 MS.gene053186:CDS 50.0%
GGGCATGTAGCAATGTCTTC+TGG + chr4.2:92254143-92254162 None:intergenic 50.0%
!! GTAGTTCTAGCTTTGAGGCG+TGG - chr4.2:92253723-92253742 MS.gene053186:CDS 50.0%
!! TAGCTTTGAGGCGTGGATCA+TGG - chr4.2:92253730-92253749 MS.gene053186:CDS 50.0%
AAGTTGCGGGTCAGAATCCC+AGG + chr4.2:92254164-92254183 None:intergenic 55.0%
AGTTGCGGGTCAGAATCCCA+GGG + chr4.2:92254163-92254182 None:intergenic 55.0%
TGGGATTCTGACCCGCAACT+TGG - chr4.2:92254163-92254182 MS.gene053186:CDS 55.0%
!! AGATTTTCGAGAGGTGGGGC+TGG - chr4.2:92254006-92254025 MS.gene053186:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 92253231 92254373 92253231 ID=MS.gene053186
chr4.2 mRNA 92253231 92254373 92253231 ID=MS.gene053186.t1;Parent=MS.gene053186
chr4.2 exon 92253231 92254373 92253231 ID=MS.gene053186.t1.exon1;Parent=MS.gene053186.t1
chr4.2 CDS 92253231 92254373 92253231 ID=cds.MS.gene053186.t1;Parent=MS.gene053186.t1
Gene Sequence

>MS.gene053186

ATGTATCATTTTCTCATTGCTAGATTCACTCATACTCATAGAACCCTTTATTCAACTACTGTTCTTTATCACAGACACAATCCTTTTTCACTTTTCTCTGCTGTTTCCAACACAAATCAACATAAAGGTGACACCTTTACATTGTCAAACCTCATAAACTCATGTGGGTTGTCTCCTGAAAAAGCCCTTAAACTTTCAACAAGGTTGCAACTCATAAACCCTAATGGCCCAAATGCTGTTATTCAGCTTTTCAGAAGCTATGGTTTCTCTGATTCTCAGTTATCAAGCCTTGTTAAGAAACACCCTTTTGTACTTTTATCAAAATCTGATAAGACCCTTTTGCCAAAACTCAAGTTTTTGCAATCCATTGGGGCTTCAACCATTGATTTCCCCAAAATCTTGATTGGTGACAAGTTTTTGACAGCAAGCTTGGAGAAAACGATAATACCTCGTTACAAGATTCTTAAAAGCCTAGTTTGCGATGATAAAAAGGTAGTTCTAGCTTTGAGGCGTGGATCATGGAACTTTTACAATGATTCCATGGTTAATGATTCTGTTCCGAACATTGAGGTTTTGAGGAAGCTTGGTGTGCCTCAAAGTTCTATTTCCAGTTTGGTATCCAATTTTCCTTGTGTAGCATTCACCAAGCATTCTAGATTTGTTGAGGCCGTTAATTCAGTCAAAGAAATGGGGTTCAATCCTTCGAAGTTGTATTTTGTTTTGGCGATTCGAGTAATTGTAAGTATGGACAAAGAAATTTGGGAATCAAAATTTAAGATTTTCGAGAGGTGGGGCTGGTCGAGAGATATTTGTCGTTTCGCTTTCCTAAAGTATCCCGAGTATGTGATGATCTCAGAGAAAAAAATTATGAAAACAATGGACTTCTTGGTGAAGGATGTGGGTCTTACACCAGAAGACATTGCTACATGCCCTGGGATTCTGACCCGCAACTTGGAGAAGACACTTGTACCTAAATGTGCTGTTATCAAGATTTTGAAGTCAAGAGGTTTAATAAAGAGTGGTTTGCGTGTAAGTACCTTTATTATGACAAGTGAGGAAAAGTTTGTGCAGAAATATGTGACCCCATTTCAGAAAGATTTGCCATTGTTATTGGATGCATATAAAGTTCAGAAATCAGATTGA
Protein sequence

>MS.gene053186.t1

MYHFLIARFTHTHRTLYSTTVLYHRHNPFSLFSAVSNTNQHKGDTFTLSNLINSCGLSPEKALKLSTRLQLINPNGPNAVIQLFRSYGFSDSQLSSLVKKHPFVLLSKSDKTLLPKLKFLQSIGASTIDFPKILIGDKFLTASLEKTIIPRYKILKSLVCDDKKVVLALRRGSWNFYNDSMVNDSVPNIEVLRKLGVPQSSISSLVSNFPCVAFTKHSRFVEAVNSVKEMGFNPSKLYFVLAIRVIVSMDKEIWESKFKIFERWGWSRDICRFAFLKYPEYVMISEKKIMKTMDFLVKDVGLTPEDIATCPGILTRNLEKTLVPKCAVIKILKSRGLIKSGLRVSTFIMTSEEKFVQKYVTPFQKDLPLLLDAYKVQKSD