AlfalfaGEDB | MS.gene053306

M. sativa cultivar XinJiangDaYe / MS.gene053306

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053306.t1	RHN41244.1	89.2	148	14	2	1	146	31	178	5.30E-66	260.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053306.t1	O48686	53.6	84	39	0	8	91	8	91	1.9e-19	97.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053306.t1	G7LEM6	89.2	148	14	2	1	146	1	148	3.8e-66	260.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene053306.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053306.t1	MTR_8g062350	87.417	151	17	2	1	149	1	151	6.27e-92	265
MS.gene053306.t1	MTR_5g036670	54.023	87	40	0	4	90	33	119	1.87e-27	107
MS.gene053306.t1	MTR_8g062700	54.118	85	39	0	6	90	34	118	1.25e-25	102
MS.gene053306.t1	MTR_8g062700	54.118	85	39	0	6	90	34	118	1.26e-25	102
MS.gene053306.t1	MTR_6g008970	49.515	103	48	2	2	103	54	153	1.21e-23	96.3
MS.gene053306.t1	MTR_6g008995	50.588	85	41	1	8	92	31	114	1.73e-21	89.7
MS.gene053306.t1	MTR_1g105955	51.389	72	35	0	7	78	9	80	2.56e-18	77.4
MS.gene053306.t1	MTR_1g105955	48.571	70	36	0	13	82	94	163	1.47e-12	62.4
MS.gene053306.t1	MTR_1g105960	46.667	75	40	0	15	89	2	76	2.61e-18	75.5
MS.gene053306.t1	MTR_3g006125	49.383	81	40	1	5	84	171	251	4.59e-17	75.5
MS.gene053306.t1	MTR_3g006125	42.056	107	55	2	8	107	83	189	4.01e-16	73.2
MS.gene053306.t1	MTR_3g006105	41.284	109	57	3	8	109	2	110	5.53e-17	73.9
MS.gene053306.t1	MTR_3g006105	44.578	83	44	2	5	85	90	172	8.55e-13	63.2
MS.gene053306.t1	MTR_3g006185	50.649	77	37	1	8	83	2	78	2.60e-16	72.0
MS.gene053306.t1	MTR_3g006185	45.556	90	41	2	7	95	91	173	3.76e-15	68.9
MS.gene053306.t1	MTR_3g006150	50.000	74	36	1	7	79	83	156	4.00e-16	71.2
MS.gene053306.t1	MTR_3g006150	39.773	88	48	2	8	93	2	86	9.11e-12	59.7
MS.gene053306.t1	MTR_1g104960	37.500	88	55	0	5	92	90	177	1.16e-15	70.5
MS.gene053306.t1	MTR_1g104960	42.254	71	41	0	13	83	12	82	1.23e-11	59.7
MS.gene053306.t1	MTR_1g104940	40.449	89	53	0	1	89	1	89	2.39e-15	68.2
MS.gene053306.t1	MTR_3g006065	48.649	74	37	1	7	79	83	156	2.63e-15	69.7
MS.gene053306.t1	MTR_4g133300	40.449	89	51	1	8	94	83	171	1.44e-14	68.9
MS.gene053306.t1	MTR_4g133300	44.156	77	41	1	7	83	167	241	7.81e-12	61.2
MS.gene053306.t1	MTR_4g133300	44.156	77	40	1	13	89	11	84	6.99e-11	58.9
MS.gene053306.t1	MTR_1g105010	35.965	114	62	2	6	113	8	116	5.20e-14	64.7
MS.gene053306.t1	MTR_7g013410	40.000	75	39	1	18	92	2	70	1.41e-13	64.3
MS.gene053306.t1	MTR_4g133470	38.202	89	53	1	8	94	83	171	5.62e-13	64.7
MS.gene053306.t1	MTR_4g133470	44.156	77	41	1	7	83	167	241	1.58e-11	60.5
MS.gene053306.t1	MTR_4g133470	44.156	77	40	1	13	89	11	84	4.87e-11	59.3
MS.gene053306.t1	MTR_6g008995	50.877	57	27	1	36	92	2	57	1.23e-12	64.3
MS.gene053306.t1	MTR_1g091047	44.776	67	36	1	13	78	8	74	8.33e-12	57.8
MS.gene053306.t1	MTR_7g013380	39.130	92	47	2	14	96	185	276	1.06e-11	61.6
MS.gene053306.t1	MTR_1g104937	38.462	78	46	1	14	89	12	89	6.71e-11	56.6
MS.gene053306.t1	MTR_2g097830	41.558	77	42	1	13	89	69	142	6.72e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053306.t1	AT1G24190	52.326	86	41	0	6	91	6	91	1.04e-24	99.4
MS.gene053306.t1	AT1G24190	52.326	86	41	0	6	91	6	91	1.14e-24	99.4
MS.gene053306.t1	AT1G24190	52.326	86	41	0	6	91	6	91	1.14e-24	99.4
MS.gene053306.t1	AT3G01320	48.421	95	49	0	7	101	50	144	4.17e-24	97.8
MS.gene053306.t1	AT3G01320	48.421	95	49	0	7	101	50	144	4.46e-24	97.4
MS.gene053306.t1	AT1G70060	47.778	90	47	0	1	90	1	90	6.43e-23	94.4
MS.gene053306.t1	AT1G70060	47.778	90	47	0	1	90	1	90	6.43e-23	94.4
MS.gene053306.t1	AT1G59890	50.000	82	41	0	14	95	45	126	1.88e-22	92.8
MS.gene053306.t1	AT1G59890	50.000	82	41	0	14	95	45	126	2.05e-22	92.8
MS.gene053306.t1	AT1G59890	50.000	82	41	0	14	95	45	126	2.07e-22	92.8
MS.gene053306.t1	AT1G59890	50.000	82	41	0	14	95	45	126	2.32e-22	92.8
MS.gene053306.t1	AT5G15020	46.875	96	50	1	8	103	46	140	9.94e-22	90.9
MS.gene053306.t1	AT5G15020	46.875	96	50	1	8	103	46	140	1.05e-21	90.9
MS.gene053306.t1	AT5G15025	50.633	79	39	0	10	88	43	121	7.04e-21	82.4
MS.gene053306.t1	AT1G10450	47.561	82	43	0	14	95	84	165	2.38e-19	84.0
MS.gene053306.t1	AT1G10450	47.561	82	43	0	14	95	33	114	2.67e-19	84.0
MS.gene053306.t1	AT1G10450	47.561	82	43	0	14	95	33	114	2.67e-19	84.0
MS.gene053306.t1	AT1G70030	44.706	85	47	0	3	87	11	95	3.68e-19	78.2
MS.gene053306.t1	AT1G70030	44.706	85	47	0	3	87	11	95	6.47e-19	78.2
MS.gene053306.t1	AT1G70030	45.122	82	45	0	6	87	3	84	2.61e-18	77.0
MS.gene053306.t1	AT5G15040	45.946	74	40	0	17	90	6	79	2.41e-17	72.4
MS.gene053306.t1	AT5G15040	45.946	74	40	0	17	90	6	79	2.41e-17	72.4
MS.gene053306.t1	AT5G15040	45.946	74	40	0	17	90	6	79	2.41e-17	72.4
MS.gene053306.t1	AT1G27220	45.570	79	42	1	5	82	4	82	6.51e-17	73.9
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.13e-16	76.3
MS.gene053306.t1	AT1G24200	44.565	92	48	1	1	92	1	89	1.18e-16	73.6
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	237	324	1.32e-16	76.3
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.33e-16	76.3
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.33e-16	76.3
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.44e-16	76.3
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.51e-16	75.9
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.55e-16	75.9
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.55e-16	75.9
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.56e-16	75.9
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.56e-16	75.9
MS.gene053306.t1	AT4G12020	43.820	89	49	1	10	98	304	391	1.59e-16	75.9
MS.gene053306.t1	AT1G27250	42.466	73	42	0	9	81	16	88	1.08e-15	69.7
MS.gene053306.t1	AT1G24210	38.462	78	43	1	10	87	10	82	4.00e-13	63.2
MS.gene053306.t1	AT1G27260	40.000	70	42	0	15	84	4	73	1.47e-12	63.2
MS.gene053306.t1	AT5G35610	38.144	97	59	1	6	102	2	97	1.18e-11	59.3
MS.gene053306.t1	AT1G23810	42.188	64	37	0	11	74	106	169	2.20e-11	60.1
MS.gene053306.t1	AT1G24250	42.188	64	37	0	11	74	117	180	5.92e-11	58.9

Find 0 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAGAAAAATGTAAATTAC+CGG	+	57420:449-468	MS.gene053306:intergenic	15.0%
!!	TGGTAAACTATAAATAATAT+AGG	+	57420:482-501	MS.gene053306:intergenic	15.0%
!!!	TTACTTTTTGAAAATAATGA+AGG	-	57420:251-270	MS.gene053306:CDS	15.0%
!!	TAATTAGAGACGAAAAAAAT+TGG	+	57420:423-442	MS.gene053306:intergenic	20.0%
!	TAAATCACTGACCAATATTT+CGG	-	57420:362-381	MS.gene053306:CDS	25.0%
!	TAATTACCATGATGTTGTAA+CGG	-	57420:571-590	MS.gene053306:intron	25.0%
!	TTTCGCAATAAATTAAGATC+CGG	+	57420:512-531	MS.gene053306:intergenic	25.0%
!!!	ATTTTTTTCGTCCGAAATAT+TGG	+	57420:376-395	MS.gene053306:intergenic	25.0%
	ACATTCATCAAGAATCTTAG+GGG	+	57420:699-718	MS.gene053306:intergenic	30.0%
	AGGACATAATGATCTGATTA+CGG	-	57420:613-632	MS.gene053306:intron	30.0%
	ATGTGAAATGACAATTCCAT+TGG	-	57420:715-734	MS.gene053306:intron	30.0%
	CACATTCATCAAGAATCTTA+GGG	+	57420:700-719	MS.gene053306:intergenic	30.0%
	GATTACAAAGATAACAGGTT+AGG	-	57420:273-292	MS.gene053306:CDS	30.0%
	TATTTATAGTTTACCACGAG+AGG	-	57420:486-505	MS.gene053306:intron	30.0%
	TCACATTCATCAAGAATCTT+AGG	+	57420:701-720	MS.gene053306:intergenic	30.0%
	TGAAGGATTACAAAGATAAC+AGG	-	57420:268-287	MS.gene053306:CDS	30.0%
!	TTTTCGTCTCTAATTAATGC+CGG	-	57420:427-446	MS.gene053306:CDS	30.0%
!!!	AGAGGTTTTAATTCTTCCAA+TGG	+	57420:734-753	MS.gene053306:intergenic	30.0%
	AATCCCAATTTCACAAGAAG+AGG	-	57420:667-686	MS.gene053306:intron	35.0%
	CATTCATCAAGAATCTTAGG+GGG	+	57420:698-717	MS.gene053306:intergenic	35.0%
	GGACATAATGATCTGATTAC+GGG	-	57420:614-633	MS.gene053306:intron	35.0%
	GGATGTCTTCAAAGAACAAA+TGG	-	57420:218-237	MS.gene053306:CDS	35.0%
	GTTCCTCTTCTTGTGAAATT+GGG	+	57420:673-692	MS.gene053306:intergenic	35.0%
	TGAGTTTGTCAGGAAAGTTA+AGG	-	57420:844-863	MS.gene053306:CDS	35.0%
	TGATGCCTTTGCATATCTTA+AGG	-	57420:188-207	MS.gene053306:CDS	35.0%
	TGTTCCTCTTCTTGTGAAAT+TGG	+	57420:674-693	MS.gene053306:intergenic	35.0%
!	ATTGGGATTTGATAACCACT+TGG	+	57420:656-675	MS.gene053306:intergenic	35.0%
!!	ATATGCAAAGGCATCATCTT+CGG	+	57420:184-203	MS.gene053306:intergenic	35.0%
	AACACGCTAATGAGTTTGTC+AGG	-	57420:834-853	MS.gene053306:CDS	40.0%
	ACAAACTCATTAGCGTGTTC+AGG	+	57420:833-852	MS.gene053306:intergenic	40.0%
	AGCATATCACAACTCCATCA+AGG	-	57420:789-808	MS.gene053306:CDS	40.0%
	CCAAATGGTTCATTCCTTGA+TGG	+	57420:806-825	MS.gene053306:intergenic	40.0%
	CCATCAAGGAATGAACCATT+TGG	-	57420:803-822	MS.gene053306:CDS	40.0%
	GACTGCCTTAAGATATGCAA+AGG	+	57420:196-215	MS.gene053306:intergenic	40.0%
	GAGGTGAAGGAGTTGTTTAA+AGG	-	57420:593-612	MS.gene053306:intron	40.0%
	GCTAAGACAATCAAGAGTGA+TGG	+	57420:327-346	MS.gene053306:intergenic	40.0%
	TCAAGGAATGAACCATTTGG+TGG	-	57420:806-825	MS.gene053306:CDS	40.0%
	TGCATATCTTAAGGCAGTCA+AGG	-	57420:197-216	MS.gene053306:CDS	40.0%
	TTACCATGATGTTGTAACGG+AGG	-	57420:574-593	MS.gene053306:intron	40.0%
	TTCAACATCTTTGCACCAAG+TGG	-	57420:638-657	MS.gene053306:intron	40.0%
!	CAAGAGTGATGGAAAGAATG+TGG	+	57420:316-335	MS.gene053306:intergenic	40.0%
	CAATCTCCTCAGATGATGCT+TGG	-	57420:761-780	MS.gene053306:CDS	45.0%
	TATAGTTTACCACGAGAGGC+CGG	-	57420:490-509	MS.gene053306:intron	45.0%
	TCACCTCCGTTACAACATCA+TGG	+	57420:580-599	MS.gene053306:intergenic	45.0%
	TGAGGAGATTGTGCATTGAG+AGG	+	57420:752-771	MS.gene053306:intergenic	45.0%
!	TGATGTTGTAACGGAGGTGA+AGG	-	57420:580-599	MS.gene053306:intron	45.0%
	AATTAAGATCCGGCCTCTCG+TGG	+	57420:502-521	MS.gene053306:intergenic	50.0%
	TCTCCACCAAGCATCATCTG+AGG	+	57420:770-789	MS.gene053306:intergenic	50.0%
	TCTCCTCAGATGATGCTTGG+TGG	-	57420:764-783	MS.gene053306:CDS	50.0%
	GCGTGTTCAGGTCCACCAAA+TGG	+	57420:821-840	MS.gene053306:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
57420	gene	150	886	150	ID=MS.gene053306
57420	mRNA	150	886	150	ID=MS.gene053306.t1;Parent=MS.gene053306
57420	exon	747	886	747	ID=MS.gene053306.t1.exon1;Parent=MS.gene053306.t1
57420	CDS	747	886	747	ID=cds.MS.gene053306.t1;Parent=MS.gene053306.t1
57420	exon	150	468	150	ID=MS.gene053306.t1.exon2;Parent=MS.gene053306.t1
57420	CDS	150	468	150	ID=cds.MS.gene053306.t1;Parent=MS.gene053306.t1

Gene Sequence

>MS.gene053306

ATGAGTACGAGTCAAGGTGGTAAGAAACTAACCGAAGATGATGCCTTTGCATATCTTAAGGCAGTCAAGGATGTCTTCAAAGAACAAATGGAAAACTATTCTTACTTTTTGAAAATAATGAAGGATTACAAAGATAACAGGTTAGGTCATTTGTTCATTTGTTCCACATTCTTTCCATCACTCTTGATTGTCTTAGCATAACTTCAAAATTTTAAATCACTGACCAATATTTCGGACGAAAAAAATAAGTAAAATAAATGTAAATTTAAACCAATTTTTTTCGTCTCTAATTAATGCCGGTAATTTACATTTTTCTATTTGTGCAATTTCCTATATTATTTATAGTTTACCACGAGAGGCCGGATCTTAATTTATTGCGAAAAAATGACTTCAATATATTTATTTTGTTGATTTCTAGAATTAATTACCATGATGTTGTAACGGAGGTGAAGGAGTTGTTTAAAGGACATAATGATCTGATTACGGGATTCAACATCTTTGCACCAAGTGGTTATCAAATCCCAATTTCACAAGAAGAGGAACAACCCCCTAAGATTCTTGATGAATGTGAAATGACAATTCCATTGGAAGAATTAAAACCTCTCAATGCACAATCTCCTCAGATGATGCTTGGTGGAGAGCATATCACAACTCCATCAAGGAATGAACCATTTGGTGGACCTGAACACGCTAATGAGTTTGTCAGGAAAGTTAAGGTAATTTTTATAAACAAATGA

Protein sequence

>MS.gene053306.t1

MSTSQGGKKLTEDDAFAYLKAVKDVFKEQMENYSYFLKIMKDYKDNRINYHDVVTEVKELFKGHNDLITGFNIFAPSGYQIPISQEEEQPPKILDECEMTIPLEELKPLNAQSPQMMLGGEHITTPSRNEPFGGPEHANEFVRKVKVIFINK