AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene053314

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053314.t1 MTR_8g062240 96.479 284 3 2 1 283 1 278 0.0 552
MS.gene053314.t1 MTR_5g037250 70.833 216 43 6 75 283 37 239 1.99e-96 283
MS.gene053314.t1 MTR_7g053410 53.659 205 73 6 82 274 234 428 1.46e-52 179
MS.gene053314.t1 MTR_7g053410 53.659 205 73 6 82 274 234 428 1.86e-52 179
MS.gene053314.t1 MTR_8g012290 63.309 139 40 3 107 237 237 372 1.08e-50 174
MS.gene053314.t1 MTR_8g012290 63.309 139 40 3 107 237 237 372 1.09e-50 174
MS.gene053314.t1 MTR_5g048860 71.930 114 30 1 132 245 343 454 2.71e-47 166
MS.gene053314.t1 MTR_8g065740 55.152 165 64 4 82 245 239 394 1.26e-46 162
MS.gene053314.t1 MTR_1g017350 51.977 177 78 3 79 255 190 359 3.23e-46 160
MS.gene053314.t1 MTR_1g017350 51.977 177 78 3 79 255 190 359 4.27e-46 160
MS.gene053314.t1 MTR_1g017350 53.988 163 68 3 79 241 190 345 8.71e-46 159
MS.gene053314.t1 MTR_1g017350 53.988 163 68 3 79 241 190 345 1.04e-45 159
MS.gene053314.t1 MTR_4g070320 55.484 155 53 2 108 249 85 236 5.20e-45 155
MS.gene053314.t1 MTR_3g116770 60.417 144 44 4 121 252 150 292 9.56e-45 156
MS.gene053314.t1 MTR_3g116770 60.417 144 44 4 121 252 150 292 9.57e-45 156
MS.gene053314.t1 MTR_8g099880 88.000 75 9 0 153 227 142 216 4.23e-40 142
MS.gene053314.t1 MTR_3g498825 82.716 81 14 0 147 227 138 218 1.17e-39 141
MS.gene053314.t1 MTR_7g092510 72.093 86 24 0 150 235 167 252 1.86e-37 135
MS.gene053314.t1 MTR_1g059270 66.667 90 30 0 153 242 157 246 2.59e-37 133
MS.gene053314.t1 MTR_1g059270 69.412 85 26 0 153 237 157 241 5.27e-36 131
MS.gene053314.t1 MTR_6g084120 41.358 162 62 5 75 236 98 226 5.55e-26 103
MS.gene053314.t1 MTR_4g131160 59.756 82 32 1 140 221 180 260 3.77e-22 95.5
MS.gene053314.t1 MTR_4g131160 59.756 82 32 1 140 221 183 263 4.18e-22 95.1
MS.gene053314.t1 MTR_1g080890 58.974 78 26 2 144 221 186 257 8.40e-20 88.2
MS.gene053314.t1 MTR_4g107320 57.692 78 25 4 174 243 60 137 5.75e-17 76.3
MS.gene053314.t1 MTR_2g098310 38.356 146 73 5 86 220 257 396 5.56e-16 77.8
MS.gene053314.t1 MTR_4g065870 33.537 164 98 3 57 220 267 419 1.12e-15 77.0
MS.gene053314.t1 MTR_1g069155 47.561 82 41 2 158 239 319 398 3.59e-15 75.5
MS.gene053314.t1 MTR_2g098310 38.621 145 72 5 86 219 257 395 4.12e-15 75.1
MS.gene053314.t1 MTR_4g065870 32.919 161 97 3 57 217 267 416 4.72e-15 75.1
MS.gene053314.t1 MTR_1g084980 50.000 64 31 1 158 221 464 526 5.81e-15 75.1
MS.gene053314.t1 MTR_1g052470 48.101 79 36 1 144 222 291 364 1.84e-13 70.1
MS.gene053314.t1 MTR_7g099540 50.000 64 31 1 158 221 254 316 3.56e-13 69.7
MS.gene053314.t1 MTR_4g033295 49.231 65 33 0 156 220 118 182 4.72e-13 66.6
MS.gene053314.t1 MTR_1g070870 60.606 66 25 1 156 221 120 184 6.47e-13 67.8
MS.gene053314.t1 MTR_7g092540 44.595 74 41 0 156 229 243 316 6.93e-13 68.2
MS.gene053314.t1 MTR_1g070870 60.606 66 25 1 156 221 123 187 7.17e-13 67.8
MS.gene053314.t1 MTR_4g033295 47.761 67 34 1 156 221 118 184 8.35e-13 65.9
MS.gene053314.t1 MTR_7g092540 48.438 64 33 0 156 219 243 306 1.67e-12 67.0
MS.gene053314.t1 MTR_1g018090 49.231 65 32 1 156 220 274 337 2.57e-12 66.6
MS.gene053314.t1 MTR_1g019240 44.615 65 35 1 158 222 87 150 5.13e-12 65.1
MS.gene053314.t1 MTR_3g103030 35.659 129 69 3 105 220 165 292 5.96e-12 65.5
MS.gene053314.t1 MTR_8g036235 47.692 65 34 0 156 220 316 380 1.10e-11 65.1
MS.gene053314.t1 MTR_5g005110 50.000 64 31 1 157 220 225 287 1.17e-11 64.7
MS.gene053314.t1 MTR_7g099540 46.875 64 33 1 158 221 254 316 1.78e-11 64.3
MS.gene053314.t1 MTR_7g111320 48.438 64 32 1 158 221 434 496 2.39e-11 64.3
MS.gene053314.t1 MTR_3g449770 46.875 64 33 1 158 221 311 373 3.28e-11 63.9
MS.gene053314.t1 MTR_2g098310 32.584 178 94 8 86 245 257 426 4.93e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene053314.t1 AT1G59640 58.419 291 86 10 1 283 1 264 4.98e-97 286
MS.gene053314.t1 AT1G59640 57.258 248 72 9 1 241 1 221 2.26e-75 234
MS.gene053314.t1 AT5G62610 51.525 295 117 13 1 283 1 281 4.14e-70 218
MS.gene053314.t1 AT5G62610 51.525 295 117 13 1 283 1 281 4.14e-70 218
MS.gene053314.t1 AT3G07340 52.151 186 59 4 64 241 180 343 2.52e-48 166
MS.gene053314.t1 AT3G07340 52.151 186 59 4 64 241 180 343 5.79e-48 166
MS.gene053314.t1 AT1G26260 56.329 158 63 2 90 246 110 262 1.10e-46 159
MS.gene053314.t1 AT1G26260 56.329 158 63 2 90 246 139 291 2.22e-46 160
MS.gene053314.t1 AT1G26260 56.329 158 63 2 90 246 161 313 3.53e-46 159
MS.gene053314.t1 AT1G26260 56.329 158 63 2 90 246 161 313 3.53e-46 159
MS.gene053314.t1 AT1G26260 56.329 158 63 2 90 246 161 313 3.53e-46 159
MS.gene053314.t1 AT1G26260 56.329 158 63 2 90 246 161 313 3.53e-46 159
MS.gene053314.t1 AT5G48560 77.451 102 19 3 141 241 289 387 8.14e-46 160
MS.gene053314.t1 AT5G48560 77.451 102 19 3 141 241 289 387 1.10e-45 160
MS.gene053314.t1 AT1G68920 52.571 175 68 3 79 244 223 391 5.11e-45 159
MS.gene053314.t1 AT1G68920 52.571 175 68 3 79 244 223 391 5.11e-45 159
MS.gene053314.t1 AT1G68920 52.571 175 68 3 79 244 223 391 5.11e-45 159
MS.gene053314.t1 AT1G68920 75.758 99 24 0 146 244 292 390 5.63e-45 158
MS.gene053314.t1 AT3G23690 61.029 136 42 3 95 230 141 265 7.93e-44 153
MS.gene053314.t1 AT5G50915 56.115 139 49 3 91 227 79 207 2.92e-43 149
MS.gene053314.t1 AT5G50915 56.115 139 49 3 91 227 79 207 2.92e-43 149
MS.gene053314.t1 AT5G50915 56.522 138 48 3 91 226 79 206 6.32e-43 148
MS.gene053314.t1 AT5G50915 56.522 138 48 3 91 226 79 206 6.32e-43 148
MS.gene053314.t1 AT1G10120 70.833 96 28 0 149 244 199 294 2.58e-42 149
MS.gene053314.t1 AT1G10120 70.833 96 28 0 149 244 199 294 2.58e-42 149
MS.gene053314.t1 AT4G36540 45.550 191 86 3 54 242 53 227 4.33e-42 147
MS.gene053314.t1 AT4G36540 46.032 189 84 3 54 240 53 225 1.07e-41 145
MS.gene053314.t1 AT2G18300 70.103 97 29 0 146 242 175 271 6.76e-41 144
MS.gene053314.t1 AT4G34530 82.500 80 14 0 150 229 166 245 1.33e-40 144
MS.gene053314.t1 AT2G18300 70.103 97 29 0 146 242 175 271 1.40e-40 144
MS.gene053314.t1 AT2G18300 71.579 95 27 0 146 240 175 269 2.07e-40 143
MS.gene053314.t1 AT2G42300 69.318 88 27 0 150 237 179 266 2.48e-38 136
MS.gene053314.t1 AT3G57800 71.591 88 25 0 150 237 198 285 8.85e-38 137
MS.gene053314.t1 AT2G42300 65.957 94 32 0 150 243 179 272 1.25e-37 135
MS.gene053314.t1 AT1G25330 48.507 134 60 2 113 244 66 192 3.37e-34 124
MS.gene053314.t1 AT1G18400 74.667 75 19 0 154 228 143 217 7.64e-34 124
MS.gene053314.t1 AT1G18400 74.667 75 19 0 154 228 143 217 1.38e-33 123
MS.gene053314.t1 AT1G73830 69.512 82 25 0 155 236 146 227 2.55e-33 122
MS.gene053314.t1 AT1G73830 69.512 82 25 0 155 236 146 227 2.72e-33 122
MS.gene053314.t1 AT3G57800 45.390 141 30 1 150 243 198 338 6.04e-29 114
MS.gene053314.t1 AT2G24260 63.014 73 26 1 149 221 28 99 1.71e-22 94.0
MS.gene053314.t1 AT2G24260 63.014 73 26 1 149 221 131 202 1.19e-21 93.6
MS.gene053314.t1 AT4G30980 68.182 66 20 1 156 221 130 194 5.51e-21 90.9
MS.gene053314.t1 AT4G30980 68.182 66 20 1 156 221 178 242 7.75e-21 91.3
MS.gene053314.t1 AT5G58010 61.842 76 28 1 146 221 89 163 7.98e-21 90.1
MS.gene053314.t1 AT2G42300 78.723 47 10 0 150 196 179 225 2.79e-19 84.7
MS.gene053314.t1 AT2G42300 78.723 47 10 0 150 196 179 225 2.79e-19 84.7
MS.gene053314.t1 AT2G42300 80.435 46 9 0 150 195 179 224 3.44e-19 84.7
MS.gene053314.t1 AT4G02590 49.515 103 46 3 124 221 46 147 1.54e-15 74.7
MS.gene053314.t1 AT4G02590 49.515 103 46 3 124 221 109 210 2.22e-15 75.1
MS.gene053314.t1 AT4G02590 49.515 103 46 3 124 221 109 210 2.22e-15 75.1
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 209 271 5.74e-15 74.7
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 209 271 5.74e-15 74.7
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 209 271 5.74e-15 74.7
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 280 342 7.63e-15 74.7
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 280 342 7.63e-15 74.7
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 280 342 7.63e-15 74.7
MS.gene053314.t1 AT2G20180 51.562 64 30 1 158 221 280 342 7.63e-15 74.7
MS.gene053314.t1 AT1G09530 50.000 64 31 1 158 221 339 401 9.09e-15 74.3
MS.gene053314.t1 AT1G09530 50.000 64 31 1 158 221 339 401 9.09e-15 74.3
MS.gene053314.t1 AT1G09530 50.000 64 31 1 158 221 339 401 9.09e-15 74.3
MS.gene053314.t1 AT1G09530 50.000 64 31 1 158 221 339 401 9.09e-15 74.3
MS.gene053314.t1 AT1G09530 50.000 64 31 1 158 221 339 401 9.09e-15 74.3
MS.gene053314.t1 AT1G09530 50.000 64 31 1 158 221 339 401 9.09e-15 74.3
MS.gene053314.t1 AT1G03040 48.544 103 47 3 124 221 107 208 2.10e-14 72.4
MS.gene053314.t1 AT1G03040 48.544 103 47 3 124 221 107 208 2.38e-14 72.4
MS.gene053314.t1 AT4G28800 44.000 75 41 1 159 233 252 325 1.53e-13 70.9
MS.gene053314.t1 AT1G03040 54.217 83 35 2 141 221 122 203 2.37e-13 69.3
MS.gene053314.t1 AT2G20180 50.820 61 29 1 158 218 280 339 3.55e-13 69.3
MS.gene053314.t1 AT2G42280 50.000 64 32 0 156 219 279 342 4.01e-13 68.9
MS.gene053314.t1 AT1G51140 53.125 64 30 0 156 219 304 367 4.43e-13 68.9
MS.gene053314.t1 AT4G28815 51.724 58 27 1 159 216 71 127 4.93e-13 67.4
MS.gene053314.t1 AT4G09180 46.269 67 36 0 154 220 182 248 8.09e-13 67.4
MS.gene053314.t1 AT2G42280 51.515 66 30 1 156 219 279 344 8.46e-13 68.2
MS.gene053314.t1 AT4G28800 45.833 72 37 2 159 229 264 334 1.17e-12 68.2
MS.gene053314.t1 AT4G28790 35.200 125 61 5 158 278 273 381 1.47e-12 67.8
MS.gene053314.t1 AT4G28815 51.724 58 27 1 159 216 147 203 1.54e-12 67.0
MS.gene053314.t1 AT2G46970 43.836 73 35 2 154 221 216 287 2.40e-12 67.0
MS.gene053314.t1 AT2G46970 43.836 73 35 2 154 221 185 256 2.59e-12 67.0
MS.gene053314.t1 AT2G46970 43.836 73 35 2 154 221 194 265 2.92e-12 66.6
MS.gene053314.t1 AT4G33880 42.857 84 45 2 139 220 247 329 4.10e-12 66.2
MS.gene053314.t1 AT1G35460 46.154 65 35 0 156 220 181 245 5.41e-12 65.1
MS.gene053314.t1 AT4G00050 47.692 65 33 1 156 220 105 168 1.41e-11 64.3
MS.gene053314.t1 AT1G27740 49.231 65 32 1 156 220 167 230 2.04e-11 63.2
MS.gene053314.t1 AT2G14760 49.231 65 32 1 156 220 237 300 2.18e-11 63.9
MS.gene053314.t1 AT4G00050 47.692 65 33 1 156 220 207 270 2.36e-11 63.9
MS.gene053314.t1 AT4G00050 47.692 65 33 1 156 220 207 270 2.73e-11 63.9
MS.gene053314.t1 AT4G36930 47.541 61 31 1 162 222 197 256 8.35e-11 62.4

Find 94 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTCTTCAATTTAGGTTAT+TGG 0.179282 8.3:+39468589 MS.gene053314:intron
TGCTTAACACTTACATCTTT+TGG 0.201350 8.3:-39468844 None:intergenic
TTTCTTCAATTTAGGTTATT+GGG 0.256415 8.3:+39468590 MS.gene053314:intron
CAGTTTCCAGCGGCGATTAA+TGG 0.284846 8.3:+39466820 MS.gene053314:CDS
TTTAGTTCCTGGTTGTAATA+AGG 0.288451 8.3:+39468349 MS.gene053314:CDS
CCGCAGTTTGGGAACGGTTT+AGG 0.291510 8.3:+39466871 MS.gene053314:CDS
GGCATTGGCACCGCCATTAC+TGG 0.302053 8.3:-39467025 None:intergenic
GACTAAGAAGGCCGCAGTTT+GGG 0.309614 8.3:+39466860 MS.gene053314:CDS
ATTCTACAGGATTTAGTTCC+TGG 0.338943 8.3:+39468338 MS.gene053314:CDS
TTAGGACAGTTTGGTGAGTT+TGG 0.345423 8.3:+39466889 MS.gene053314:CDS
AATGGCGGCGGAGGTGGATT+AGG 0.359141 8.3:+39466838 MS.gene053314:CDS
CGTTTAGTTTAGCGGAGATC+TGG 0.375505 8.3:+39466797 MS.gene053314:CDS
CAACAAACGTTTGATCTTGC+CGG 0.377074 8.3:+39469406 MS.gene053314:CDS
ATTACCATCCCGGTTTAAAC+CGG 0.385440 8.3:-39466914 None:intergenic
ACCTGCATATGTAACCATTC+TGG 0.392490 8.3:-39469478 None:intergenic
CCCTCGTTCATCATCGCTCC+TGG 0.400423 8.3:-39466742 None:intergenic
ACATCCATGTTCGAGCTAGA+AGG 0.403285 8.3:+39467924 MS.gene053314:CDS
TGGACAATAAACAGGCTAGA+AGG 0.405648 8.3:+39468291 MS.gene053314:intron
CAGATCTCCGCTAAACTAAA+CGG 0.416576 8.3:-39466796 None:intergenic
GTTGAAGGAGAAGTCAGTTC+AGG 0.418689 8.3:+39467845 MS.gene053314:CDS
GCGATTAATGGCGGCGGAGG+TGG 0.426598 8.3:+39466832 MS.gene053314:CDS
AGTTTGGACCCGGTTTAAAC+CGG 0.426792 8.3:+39466905 MS.gene053314:CDS
GGACTAAGAAGGCCGCAGTT+TGG 0.428490 8.3:+39466859 MS.gene053314:CDS
CAGTTTGGTGAGTTTGGACC+CGG 0.446797 8.3:+39466895 MS.gene053314:CDS
GGAAAACGGACTAAAGCATC+AGG 0.450766 8.3:+39467794 MS.gene053314:CDS
CTTGTAGCTTGTGAAACAAA+TGG 0.451137 8.3:-39469433 None:intergenic
GGTTTAAACCGGGATGGTAA+TGG 0.452515 8.3:+39466916 MS.gene053314:CDS
AAGCAGTAAGCCACCTCCTT+CGG 0.454624 8.3:+39467883 MS.gene053314:CDS
TACGACGCCGTTTAGTTTAG+CGG 0.455033 8.3:+39466789 MS.gene053314:CDS
TGAAAGGATGAAAATTCTAC+AGG 0.461627 8.3:+39468325 MS.gene053314:CDS
TAGTGAACCGAAATGGATCC+AGG 0.464407 8.3:+39466724 None:intergenic
GTCGTATTTCCATTCCCATT+CGG 0.470342 8.3:-39466772 None:intergenic
CATCCATGTTCGAGCTAGAA+GGG 0.474849 8.3:+39467925 MS.gene053314:CDS
GGTTCATTTCCGAATGGGAA+TGG 0.479304 8.3:+39466763 MS.gene053314:CDS
GGGAACGGTTTAGGACAGTT+TGG 0.481836 8.3:+39466880 MS.gene053314:CDS
ACCAGAATGGTTACATATGC+AGG 0.483200 8.3:+39469477 MS.gene053314:CDS
ACATGGATGTAATCTTGCTT+AGG 0.502301 8.3:-39467911 None:intergenic
ACGAGGGTTCATTTCCGAAT+GGG 0.502721 8.3:+39466758 MS.gene053314:CDS
AACAAGAATGAGGGTGGTGA+TGG 0.502949 8.3:+39467818 MS.gene053314:CDS
CCGCCGCCATTAATCGCCGC+TGG 0.503828 8.3:-39466826 None:intergenic
GCGGCGATTAATGGCGGCGG+AGG 0.511591 8.3:+39466829 MS.gene053314:CDS
GCAGAGGTTCTTCACCAGAA+TGG 0.515410 8.3:+39469464 MS.gene053314:CDS
TGGATGTAATCTTGCTTAGG+AGG 0.520256 8.3:-39467908 None:intergenic
TGGAGGAAAGGCTTCAATGC+CGG 0.520377 8.3:-39468824 None:intergenic
ACTCCGGTCCATTACCATCC+CGG 0.528802 8.3:-39466924 None:intergenic
TTAGGTTATTGGGAAAGCAT+TGG 0.529281 8.3:+39468600 MS.gene053314:intron
GTTTGGACCCGGTTTAAACC+GGG 0.537559 8.3:+39466906 MS.gene053314:CDS
TTAGGAGGGTCCGAAGGAGG+TGG 0.538656 8.3:-39467893 None:intergenic
TCTGGTGAAGAACCTCTGCT+TGG 0.539168 8.3:-39469460 None:intergenic
GGACAATAAACAGGCTAGAA+GGG 0.544423 8.3:+39468292 MS.gene053314:intron
TTACCATCCCGGTTTAAACC+GGG 0.553757 8.3:-39466913 None:intergenic
CCAGCGGCGATTAATGGCGG+CGG 0.557594 8.3:+39466826 MS.gene053314:CDS
TTACATATGCAGGTAGGTGG+TGG 0.558363 8.3:+39469487 MS.gene053314:CDS
AGAAGGCCGCAGTTTGGGAA+CGG 0.559428 8.3:+39466865 MS.gene053314:CDS
AGCATCAGGGAACAAGAATG+AGG 0.561192 8.3:+39467808 MS.gene053314:CDS
AGAATGATAGTGATGGAAAA+CGG 0.563521 8.3:+39467780 MS.gene053314:intron
AACGAGGGTTCATTTCCGAA+TGG 0.564078 8.3:+39466757 MS.gene053314:CDS
TTGTGAAACAAATGGCATGC+CGG 0.566973 8.3:-39469425 None:intergenic
AAGTGTGTTTCAACCAGTAA+TGG 0.574093 8.3:+39467012 MS.gene053314:CDS
GACCCCTTCTAGCTCGAACA+TGG 0.574237 8.3:-39467928 None:intergenic
AAACCGGGATGGTAATGGAC+CGG 0.576710 8.3:+39466921 MS.gene053314:CDS
GAAGGGAAAAGATTAGTGAA+AGG 0.577890 8.3:+39468309 MS.gene053314:CDS
GGTGGTGATGGTAAAGTTGA+AGG 0.578873 8.3:+39467830 MS.gene053314:CDS
TTTCCAGCGGCGATTAATGG+CGG 0.585609 8.3:+39466823 MS.gene053314:CDS
TGGTAAGAAGAGGCGTGACT+CGG 0.586133 8.3:+39466975 MS.gene053314:CDS
GTTAATTCAAGACTCAATAC+CGG 0.586424 8.3:+39468805 MS.gene053314:CDS
ATCACTTCAGCGCCAAGTGG+AGG 0.597164 8.3:+39468651 MS.gene053314:CDS
TGCTTAGGAGGGTCCGAAGG+AGG 0.608784 8.3:-39467896 None:intergenic
TTCAAAACAACTCTTGACTC+CGG 0.616543 8.3:-39466940 None:intergenic
AACATTACCTTATTACAACC+AGG 0.621430 8.3:-39468356 None:intergenic
TCTTCTTACCACCACCAACA+TGG 0.628208 8.3:-39466963 None:intergenic
GCATCAGGGAACAAGAATGA+GGG 0.629320 8.3:+39467809 MS.gene053314:CDS
ATGTTGGTGGTGGTAAGAAG+AGG 0.629622 8.3:+39466965 MS.gene053314:CDS
GGATGTAATCTTGCTTAGGA+GGG 0.631066 8.3:-39467907 None:intergenic
TCCAGGAGCGATGATGAACG+AGG 0.631852 8.3:+39466741 MS.gene053314:CDS
GAGGTGGATTAGGACTAAGA+AGG 0.632789 8.3:+39466848 MS.gene053314:CDS
TAATGGCGGTGCCAATGCCG+TGG 0.636011 8.3:+39467029 MS.gene053314:CDS
TCTTGCTTAGGAGGGTCCGA+AGG 0.636318 8.3:-39467899 None:intergenic
ATCCATGTTCGAGCTAGAAG+GGG 0.638122 8.3:+39467926 MS.gene053314:CDS
GGACCCGGTTTAAACCGGGA+TGG 0.638557 8.3:+39466910 MS.gene053314:CDS
GCTACAAGAGAGCCAAGCAG+AGG 0.640109 8.3:+39469448 MS.gene053314:CDS
TGGTTACATATGCAGGTAGG+TGG 0.652770 8.3:+39469484 MS.gene053314:CDS
GGAGATCTGGCAGTTTCCAG+CGG 0.658016 8.3:+39466810 MS.gene053314:CDS
TCAATCACTTCAGCGCCAAG+TGG 0.659625 8.3:+39468648 MS.gene053314:CDS
ATAGAATAGATACCTCCACT+TGG 0.671733 8.3:-39468663 None:intergenic
TCAGGGAACAAGAATGAGGG+TGG 0.675624 8.3:+39467812 MS.gene053314:CDS
AGTCAGTTCAGGAAAGCATG+CGG 0.677155 8.3:+39467856 MS.gene053314:CDS
TGTGTTTCAACCAGTAATGG+CGG 0.680302 8.3:+39467015 MS.gene053314:CDS
GAATGGTTACATATGCAGGT+AGG 0.689550 8.3:+39469481 MS.gene053314:CDS
CCAGGAGCGATGATGAACGA+GGG 0.693487 8.3:+39466742 MS.gene053314:CDS
TTACGCGCTCTGCAAGACTG+TGG 0.695452 8.3:-39467964 None:intergenic
GAAAACGGACTAAAGCATCA+GGG 0.713041 8.3:+39467795 MS.gene053314:CDS
CCTAAACCGTTCCCAAACTG+CGG 0.713413 8.3:-39466871 None:intergenic
ATTGTGCAGAATGATAGTGA+TGG 0.726537 8.3:+39467773 MS.gene053314:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACTAAATTATTACAATAA+AGG - chr8.3:39467688-39467707 None:intergenic 10.0%
!! TTAATGGAATTATTATTATT+TGG + chr8.3:39467750-39467769 MS.gene053314:intron 10.0%
!! TTTAATGAATATACTTAAAA+CGG + chr8.3:39467215-39467234 MS.gene053314:intron 10.0%
!!! AGTTTTTTTTTAGTTATATT+TGG + chr8.3:39467076-39467095 MS.gene053314:intron 10.0%
!!! TATTAAATATATGTATTTTG+GGG + chr8.3:39467154-39467173 MS.gene053314:intron 10.0%
!!! TGTGTTTTTTATTTTATTAT+TGG + chr8.3:39467301-39467320 MS.gene053314:intron 10.0%
!! AGTTTCAATATAAAAATCAA+GGG - chr8.3:39468871-39468890 None:intergenic 15.0%
!! TTAAGGAAAATGTAAATTTA+TGG + chr8.3:39468220-39468239 MS.gene053314:intron 15.0%
!! TTTAGTAATTTACTTTCATT+TGG - chr8.3:39468460-39468479 None:intergenic 15.0%
!!! TTCACTTTTGATTTTAAAAA+TGG + chr8.3:39467478-39467497 MS.gene053314:intron 15.0%
!!! TTTTTTTAGTTATATTTGGA+AGG + chr8.3:39467080-39467099 MS.gene053314:intron 15.0%
!! ATATGTGTATTTGATGTATT+TGG + chr8.3:39467711-39467730 MS.gene053314:intron 20.0%
!! CTAAAAGATTTGTAAGTAAA+GGG - chr8.3:39467994-39468013 None:intergenic 20.0%
!! GAAAGTAAATTACTAAAAGT+AGG + chr8.3:39468463-39468482 MS.gene053314:intron 20.0%
!! GAGTTTCAATATAAAAATCA+AGG - chr8.3:39468872-39468891 None:intergenic 20.0%
!! TCTAAAAGATTTGTAAGTAA+AGG - chr8.3:39467995-39468014 None:intergenic 20.0%
!!! AACTGTTATTTTCTTTTCAT+GGG - chr8.3:39468507-39468526 None:intergenic 20.0%
!!! AATTTTTCTCCAGAATAATT+AGG - chr8.3:39467665-39467684 None:intergenic 20.0%
!!! AGGAAAATTTTCAAATAAAG+AGG - chr8.3:39468921-39468940 None:intergenic 20.0%
!!! ATGATGTTAAATCTTGTTTA+AGG + chr8.3:39468203-39468222 MS.gene053314:intron 20.0%
!!! GATTTTGCTTCATTTTTTTT+TGG + chr8.3:39467350-39467369 MS.gene053314:intron 20.0%
!!! GGAAAATTTTCAAATAAAGA+GGG - chr8.3:39468920-39468939 None:intergenic 20.0%
!!! TAACTGTTATTTTCTTTTCA+TGG - chr8.3:39468508-39468527 None:intergenic 20.0%
!!! TTTCTTCAATTTAGGTTATT+GGG + chr8.3:39468590-39468609 MS.gene053314:intron 20.0%
! ATTATAACTGACCAACAATT+AGG - chr8.3:39468538-39468557 None:intergenic 25.0%
! ATTGAGACTTTCTTCAATTT+AGG + chr8.3:39468582-39468601 MS.gene053314:intron 25.0%
! CATCATTGTTTCAAAAACAT+TGG + chr8.3:39469341-39469360 MS.gene053314:intron 25.0%
! GAAAGAGAGAAAAAAAAACT+AGG - chr8.3:39469377-39469396 None:intergenic 25.0%
! GAAGAAAAAAATGAATACCA+CGG - chr8.3:39467049-39467068 None:intergenic 25.0%
! GATAAATATACTTGATTGAG+AGG + chr8.3:39469268-39469287 MS.gene053314:intron 25.0%
! TCACAAAACGATAGAAAATT+GGG - chr8.3:39467602-39467621 None:intergenic 25.0%
! TTAACAATTAGACAGACAAA+AGG - chr8.3:39468043-39468062 None:intergenic 25.0%
!! CATTGTTCAACTTGTATTTT+TGG + chr8.3:39467257-39467276 MS.gene053314:intron 25.0%
!! CTTTCTTCAATTTAGGTTAT+TGG + chr8.3:39468589-39468608 MS.gene053314:intron 25.0%
!! TTGATGTATTTGGAATTAGT+CGG + chr8.3:39467721-39467740 MS.gene053314:intron 25.0%
!!! ACTCTTGTGTTATTGTTTTT+AGG + chr8.3:39468016-39468035 MS.gene053314:intron 25.0%
!!! ATCTATTCTATTTCTGACTT+TGG + chr8.3:39468675-39468694 MS.gene053314:intron 25.0%
!!! CTTCATTTTTTTTTGGTACA+AGG + chr8.3:39467357-39467376 MS.gene053314:intron 25.0%
!!! GTAAATTTATGGTTTTGTTG+CGG + chr8.3:39468231-39468250 MS.gene053314:intron 25.0%
!!! TCAAATTTTTGTTTCGAACT+TGG + chr8.3:39467635-39467654 MS.gene053314:intron 25.0%
AAATGAAGCAAAATCTTGAG+CGG - chr8.3:39467345-39467364 None:intergenic 30.0%
AACATTACCTTATTACAACC+AGG - chr8.3:39468359-39468378 None:intergenic 30.0%
AACATTCCAATTAGAAACAG+AGG - chr8.3:39468269-39468288 None:intergenic 30.0%
AATTAGTCGGTGTGATTTAA+TGG + chr8.3:39467734-39467753 MS.gene053314:intron 30.0%
ATTTCTCAACATCAAGAGTT+AGG + chr8.3:39468166-39468185 MS.gene053314:intron 30.0%
GTCACAAAACGATAGAAAAT+TGG - chr8.3:39467603-39467622 None:intergenic 30.0%
GTTAATTCAAGACTCAATAC+CGG + chr8.3:39468805-39468824 MS.gene053314:CDS 30.0%
GTTATGATCTTCCTAATTGT+TGG + chr8.3:39468524-39468543 MS.gene053314:intron 30.0%
TACAGCTAAAGATTGAACAT+TGG - chr8.3:39469245-39469264 None:intergenic 30.0%
TGAAAGGATGAAAATTCTAC+AGG + chr8.3:39468325-39468344 MS.gene053314:CDS 30.0%
TGAACATTGGATTAATCCAA+GGG - chr8.3:39469232-39469251 None:intergenic 30.0%
TGCTCTCATATTCACATAAA+AGG + chr8.3:39468401-39468420 MS.gene053314:intron 30.0%
TGTTGCAAGTATGTATAACA+AGG + chr8.3:39468996-39469015 MS.gene053314:intron 30.0%
TTGAACATTGGATTAATCCA+AGG - chr8.3:39469233-39469252 None:intergenic 30.0%
! AGAATGATAGTGATGGAAAA+CGG + chr8.3:39467780-39467799 MS.gene053314:intron 30.0%
! CAACATAAGGACCAAAATTT+TGG + chr8.3:39467556-39467575 MS.gene053314:intron 30.0%
! GAGGATACAGATTTAAAGTT+TGG + chr8.3:39469287-39469306 MS.gene053314:intron 30.0%
! TGCTTAACACTTACATCTTT+TGG - chr8.3:39468847-39468866 None:intergenic 30.0%
! TTAACACTTACATCTTTTGG+AGG - chr8.3:39468844-39468863 None:intergenic 30.0%
! TTCACAAACTAATTTGCCTA+TGG + chr8.3:39467424-39467443 MS.gene053314:intron 30.0%
! TTTAGTTCCTGGTTGTAATA+AGG + chr8.3:39468349-39468368 MS.gene053314:CDS 30.0%
!! TATTTTGGGGAGTGATTAAT+TGG + chr8.3:39467167-39467186 MS.gene053314:intron 30.0%
!! TCTGTTTCTAATTGGAATGT+TGG + chr8.3:39468268-39468287 MS.gene053314:intron 30.0%
!!! TTTTTTCTCTCTTTCCAGTA+TGG + chr8.3:39469382-39469401 MS.gene053314:intron 30.0%
AAAAATGAATACCACGGCAT+TGG - chr8.3:39467043-39467062 None:intergenic 35.0%
AAGTGTGTTTCAACCAGTAA+TGG + chr8.3:39467012-39467031 MS.gene053314:CDS 35.0%
AATGAAGCAAAATCTTGAGC+GGG - chr8.3:39467344-39467363 None:intergenic 35.0%
AATGGTGTGTGAACTTATTC+AGG + chr8.3:39467496-39467515 MS.gene053314:intron 35.0%
ACATGGATGTAATCTTGCTT+AGG - chr8.3:39467914-39467933 None:intergenic 35.0%
ACTCTTGATGTTGAGAAATG+CGG - chr8.3:39468165-39468184 None:intergenic 35.0%
ATAGAATAGATACCTCCACT+TGG - chr8.3:39468666-39468685 None:intergenic 35.0%
ATCTTGTGCGTGAATAATGT+CGG - chr8.3:39469037-39469056 None:intergenic 35.0%
CTTGGTTGTCCTAATTATTC+TGG + chr8.3:39467653-39467672 MS.gene053314:intron 35.0%
GAAGGGAAAAGATTAGTGAA+AGG + chr8.3:39468309-39468328 MS.gene053314:CDS 35.0%
GATGTCTTTGCTACAACATA+AGG + chr8.3:39467543-39467562 MS.gene053314:intron 35.0%
GTTTCTAATTGGAATGTTGG+TGG + chr8.3:39468271-39468290 MS.gene053314:intron 35.0%
TGAATAATGTCGGCAAAAGA+TGG - chr8.3:39469027-39469046 None:intergenic 35.0%
TGTTCAATCTTTAGCTGTAG+AGG + chr8.3:39469246-39469265 MS.gene053314:intron 35.0%
TGTTGAGATTACACTTCCAT+AGG - chr8.3:39467443-39467462 None:intergenic 35.0%
TTCAAAACAACTCTTGACTC+CGG - chr8.3:39466943-39466962 None:intergenic 35.0%
! AATGTTGGTGGACAATAAAC+AGG + chr8.3:39468283-39468302 MS.gene053314:intron 35.0%
! ATTCTACAGGATTTAGTTCC+TGG + chr8.3:39468338-39468357 MS.gene053314:CDS 35.0%
! ATTGTGCAGAATGATAGTGA+TGG + chr8.3:39467773-39467792 MS.gene053314:intron 35.0%
! ATTTTCCTTCTGACAAGACA+TGG + chr8.3:39468933-39468952 MS.gene053314:intron 35.0%
! CTTGTAGCTTGTGAAACAAA+TGG - chr8.3:39469436-39469455 None:intergenic 35.0%
! TGTGTGAACTTATTCAGGTT+TGG + chr8.3:39467501-39467520 MS.gene053314:intron 35.0%
! TTAGGTTATTGGGAAAGCAT+TGG + chr8.3:39468600-39468619 MS.gene053314:intron 35.0%
!! ACTTACATCTTTTGGAGGAA+AGG - chr8.3:39468839-39468858 None:intergenic 35.0%
!! TCTATGCCTCTGTTTCTAAT+TGG + chr8.3:39468260-39468279 MS.gene053314:intron 35.0%
!!! AGAGTTGTTTTGAACCATGT+TGG + chr8.3:39466949-39466968 MS.gene053314:CDS 35.0%
ACCAGAATGGTTACATATGC+AGG + chr8.3:39469477-39469496 MS.gene053314:CDS 40.0%
ACCTGCATATGTAACCATTC+TGG - chr8.3:39469481-39469500 None:intergenic 40.0%
ACTCACGAGTATAGTAGTTC+TGG + chr8.3:39468964-39468983 MS.gene053314:intron 40.0%
ATGAATGATAGCTTCTCGCT+AGG + chr8.3:39469128-39469147 MS.gene053314:intron 40.0%
ATTACCATCCCGGTTTAAAC+CGG - chr8.3:39466917-39466936 None:intergenic 40.0%
CAACAAACGTTTGATCTTGC+CGG + chr8.3:39469406-39469425 MS.gene053314:CDS 40.0%
CAGATCTCCGCTAAACTAAA+CGG - chr8.3:39466799-39466818 None:intergenic 40.0%
CTATGATGTCACAGCCAAAA+CGG - chr8.3:39468110-39468129 None:intergenic 40.0%
GAATGGTTACATATGCAGGT+AGG + chr8.3:39469481-39469500 MS.gene053314:CDS 40.0%
GGACAATAAACAGGCTAGAA+GGG + chr8.3:39468292-39468311 MS.gene053314:intron 40.0%
GGATGTAATCTTGCTTAGGA+GGG - chr8.3:39467910-39467929 None:intergenic 40.0%
GTCGTATTTCCATTCCCATT+CGG - chr8.3:39466775-39466794 None:intergenic 40.0%
TGGACAATAAACAGGCTAGA+AGG + chr8.3:39468291-39468310 MS.gene053314:intron 40.0%
TGGATGTAATCTTGCTTAGG+AGG - chr8.3:39467911-39467930 None:intergenic 40.0%
TGTGTTTCAACCAGTAATGG+CGG + chr8.3:39467015-39467034 MS.gene053314:CDS 40.0%
TTGTGAAACAAATGGCATGC+CGG - chr8.3:39469428-39469447 None:intergenic 40.0%
TTTAAGAAGCTTGCAACCTC+TGG + chr8.3:39469171-39469190 MS.gene053314:intron 40.0%
! AGTAGGAAAAACGTGCTACT+TGG + chr8.3:39468480-39468499 MS.gene053314:intron 40.0%
! CAAACGTTTGTTGACCATAC+TGG - chr8.3:39469399-39469418 None:intergenic 40.0%
! GAGCGAAGTAGCCAAAATTT+TGG - chr8.3:39467570-39467589 None:intergenic 40.0%
! TTAGGACAGTTTGGTGAGTT+TGG + chr8.3:39466889-39466908 MS.gene053314:CDS 40.0%
!! GAAAACGGACTAAAGCATCA+GGG + chr8.3:39467795-39467814 MS.gene053314:CDS 40.0%
!! GTAGGAAAAACGTGCTACTT+GGG + chr8.3:39468481-39468500 MS.gene053314:intron 40.0%
!!! GTTGTTTTGAACCATGTTGG+TGG + chr8.3:39466952-39466971 MS.gene053314:CDS 40.0%
AACAAGAATGAGGGTGGTGA+TGG + chr8.3:39467818-39467837 MS.gene053314:CDS 45.0%
AACCTCTGGTCTCTGCATAA+TGG + chr8.3:39469185-39469204 MS.gene053314:intron 45.0%
AACGAGGGTTCATTTCCGAA+TGG + chr8.3:39466757-39466776 MS.gene053314:CDS 45.0%
ACATCCATGTTCGAGCTAGA+AGG + chr8.3:39467924-39467943 MS.gene053314:CDS 45.0%
ACCTCTGGTCTCTGCATAAT+GGG + chr8.3:39469186-39469205 MS.gene053314:intron 45.0%
ACGAGGGTTCATTTCCGAAT+GGG + chr8.3:39466758-39466777 MS.gene053314:CDS 45.0%
AGCATCAGGGAACAAGAATG+AGG + chr8.3:39467808-39467827 MS.gene053314:CDS 45.0%
AGTTTGGACCCGGTTTAAAC+CGG + chr8.3:39466905-39466924 MS.gene053314:CDS 45.0%
ATCCATGTTCGAGCTAGAAG+GGG + chr8.3:39467926-39467945 MS.gene053314:CDS 45.0%
CATCCATGTTCGAGCTAGAA+GGG + chr8.3:39467925-39467944 MS.gene053314:CDS 45.0%
GCATCAGGGAACAAGAATGA+GGG + chr8.3:39467809-39467828 MS.gene053314:CDS 45.0%
GGTTCATTTCCGAATGGGAA+TGG + chr8.3:39466763-39466782 MS.gene053314:CDS 45.0%
GGTTTAAACCGGGATGGTAA+TGG + chr8.3:39466916-39466935 MS.gene053314:CDS 45.0%
GTTGAAGGAGAAGTCAGTTC+AGG + chr8.3:39467845-39467864 MS.gene053314:CDS 45.0%
TCTTCTTACCACCACCAACA+TGG - chr8.3:39466966-39466985 None:intergenic 45.0%
TGGTTACATATGCAGGTAGG+TGG + chr8.3:39469484-39469503 MS.gene053314:CDS 45.0%
TTACATATGCAGGTAGGTGG+TGG + chr8.3:39469487-39469506 MS.gene053314:CDS 45.0%
TTACCATCCCGGTTTAAACC+GGG - chr8.3:39466916-39466935 None:intergenic 45.0%
! AGTATTGAACTCTCAGCCCT+TGG + chr8.3:39469213-39469232 MS.gene053314:intron 45.0%
! AGTCAGTTCAGGAAAGCATG+CGG + chr8.3:39467856-39467875 MS.gene053314:CDS 45.0%
! CAGGTTTGGTCGCAATTTCA+AGG + chr8.3:39467515-39467534 MS.gene053314:intron 45.0%
! CGTTTAGTTTAGCGGAGATC+TGG + chr8.3:39466797-39466816 MS.gene053314:CDS 45.0%
! TACGACGCCGTTTAGTTTAG+CGG + chr8.3:39466789-39466808 MS.gene053314:CDS 45.0%
!! ATGTTGGTGGTGGTAAGAAG+AGG + chr8.3:39466965-39466984 MS.gene053314:CDS 45.0%
!! GAGGTGGATTAGGACTAAGA+AGG + chr8.3:39466848-39466867 MS.gene053314:CDS 45.0%
!! GGAAAACGGACTAAAGCATC+AGG + chr8.3:39467794-39467813 MS.gene053314:CDS 45.0%
!! GGTGGTGATGGTAAAGTTGA+AGG + chr8.3:39467830-39467849 MS.gene053314:CDS 45.0%
!! GTTTTGAACCATGTTGGTGG+TGG + chr8.3:39466955-39466974 MS.gene053314:CDS 45.0%
!!! CGTTTTGGCTGTGACATCAT+AGG + chr8.3:39468108-39468127 MS.gene053314:intron 45.0%
!!! AATATTAAATATATGTATTT+TGG + chr8.3:39467152-39467171 MS.gene053314:intron 5.0%
!!! ATATTAAATATATGTATTTT+GGG + chr8.3:39467153-39467172 MS.gene053314:intron 5.0%
AAGCAGTAAGCCACCTCCTT+CGG + chr8.3:39467883-39467902 MS.gene053314:CDS 50.0%
CAGTTTCCAGCGGCGATTAA+TGG + chr8.3:39466820-39466839 MS.gene053314:CDS 50.0%
CCTAAACCGTTCCCAAACTG+CGG - chr8.3:39466874-39466893 None:intergenic 50.0%
GACTAAGAAGGCCGCAGTTT+GGG + chr8.3:39466860-39466879 MS.gene053314:CDS 50.0%
GCAGAGGTTCTTCACCAGAA+TGG + chr8.3:39469464-39469483 MS.gene053314:CDS 50.0%
GTTTGGACCCGGTTTAAACC+GGG + chr8.3:39466906-39466925 MS.gene053314:CDS 50.0%
TCAATCACTTCAGCGCCAAG+TGG + chr8.3:39468648-39468667 MS.gene053314:CDS 50.0%
TCAGGGAACAAGAATGAGGG+TGG + chr8.3:39467812-39467831 MS.gene053314:CDS 50.0%
TCTGGTGAAGAACCTCTGCT+TGG - chr8.3:39469463-39469482 None:intergenic 50.0%
TGGAGGAAAGGCTTCAATGC+CGG - chr8.3:39468827-39468846 None:intergenic 50.0%
TTTCCAGCGGCGATTAATGG+CGG + chr8.3:39466823-39466842 MS.gene053314:CDS 50.0%
! CAGTTTGGTGAGTTTGGACC+CGG + chr8.3:39466895-39466914 MS.gene053314:CDS 50.0%
! GGGAACGGTTTAGGACAGTT+TGG + chr8.3:39466880-39466899 MS.gene053314:CDS 50.0%
! TGGTAAGAAGAGGCGTGACT+CGG + chr8.3:39466975-39466994 MS.gene053314:CDS 50.0%
!! AAACCGGGATGGTAATGGAC+CGG + chr8.3:39466921-39466940 MS.gene053314:CDS 50.0%
!!! TGAGTTGAGAAGCGCCGTTT+TGG + chr8.3:39468093-39468112 MS.gene053314:intron 50.0%
ACTCCGGTCCATTACCATCC+CGG - chr8.3:39466927-39466946 None:intergenic 55.0%
AGAAGGCCGCAGTTTGGGAA+CGG + chr8.3:39466865-39466884 MS.gene053314:CDS 55.0%
ATCACTTCAGCGCCAAGTGG+AGG + chr8.3:39468651-39468670 MS.gene053314:CDS 55.0%
CCAGGAGCGATGATGAACGA+GGG + chr8.3:39466742-39466761 MS.gene053314:CDS 55.0%
CCGCAGTTTGGGAACGGTTT+AGG + chr8.3:39466871-39466890 MS.gene053314:CDS 55.0%
GCCCATTATGCAGAGACCAG+AGG - chr8.3:39469190-39469209 None:intergenic 55.0%
GCGAGCCATGTCTTGTCAGA+AGG - chr8.3:39468941-39468960 None:intergenic 55.0%
GCTACAAGAGAGCCAAGCAG+AGG + chr8.3:39469448-39469467 MS.gene053314:CDS 55.0%
GGACTAAGAAGGCCGCAGTT+TGG + chr8.3:39466859-39466878 MS.gene053314:CDS 55.0%
GGAGATCTGGCAGTTTCCAG+CGG + chr8.3:39466810-39466829 MS.gene053314:CDS 55.0%
TCCAGGAGCGATGATGAACG+AGG + chr8.3:39466741-39466760 MS.gene053314:CDS 55.0%
TCTTGCTTAGGAGGGTCCGA+AGG - chr8.3:39467902-39467921 None:intergenic 55.0%
TTACGCGCTCTGCAAGACTG+TGG - chr8.3:39467967-39467986 None:intergenic 55.0%
!! GACCCCTTCTAGCTCGAACA+TGG - chr8.3:39467931-39467950 None:intergenic 55.0%
CCCTCGTTCATCATCGCTCC+TGG - chr8.3:39466745-39466764 None:intergenic 60.0%
GGACCCGGTTTAAACCGGGA+TGG + chr8.3:39466910-39466929 MS.gene053314:CDS 60.0%
TGCTTAGGAGGGTCCGAAGG+AGG - chr8.3:39467899-39467918 None:intergenic 60.0%
TTAGGAGGGTCCGAAGGAGG+TGG - chr8.3:39467896-39467915 None:intergenic 60.0%
! AATGGCGGCGGAGGTGGATT+AGG + chr8.3:39466838-39466857 MS.gene053314:CDS 60.0%
! TAATGGCGGTGCCAATGCCG+TGG + chr8.3:39467029-39467048 MS.gene053314:CDS 60.0%
!! GGCATTGGCACCGCCATTAC+TGG - chr8.3:39467028-39467047 None:intergenic 60.0%
CCAGCGGCGATTAATGGCGG+CGG + chr8.3:39466826-39466845 MS.gene053314:CDS 65.0%
GCGATTAATGGCGGCGGAGG+TGG + chr8.3:39466832-39466851 MS.gene053314:CDS 65.0%
CCGCCGCCATTAATCGCCGC+TGG - chr8.3:39466829-39466848 None:intergenic 70.0%
GCGGCGATTAATGGCGGCGG+AGG + chr8.3:39466829-39466848 MS.gene053314:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 39466736 39469528 39466736 ID=MS.gene053314
chr8.3 mRNA 39466736 39469528 39466736 ID=MS.gene053314.t1;Parent=MS.gene053314
chr8.3 exon 39466736 39467050 39466736 ID=MS.gene053314.t1.exon1;Parent=MS.gene053314.t1
chr8.3 CDS 39466736 39467050 39466736 ID=cds.MS.gene053314.t1;Parent=MS.gene053314.t1
chr8.3 exon 39467782 39467982 39467782 ID=MS.gene053314.t1.exon2;Parent=MS.gene053314.t1
chr8.3 CDS 39467782 39467982 39467782 ID=cds.MS.gene053314.t1;Parent=MS.gene053314.t1
chr8.3 exon 39468305 39468370 39468305 ID=MS.gene053314.t1.exon3;Parent=MS.gene053314.t1
chr8.3 CDS 39468305 39468370 39468305 ID=cds.MS.gene053314.t1;Parent=MS.gene053314.t1
chr8.3 exon 39468604 39468672 39468604 ID=MS.gene053314.t1.exon4;Parent=MS.gene053314.t1
chr8.3 CDS 39468604 39468672 39468604 ID=cds.MS.gene053314.t1;Parent=MS.gene053314.t1
chr8.3 exon 39468781 39468852 39468781 ID=MS.gene053314.t1.exon5;Parent=MS.gene053314.t1
chr8.3 CDS 39468781 39468852 39468781 ID=cds.MS.gene053314.t1;Parent=MS.gene053314.t1
chr8.3 exon 39469400 39469528 39469400 ID=MS.gene053314.t1.exon6;Parent=MS.gene053314.t1
chr8.3 CDS 39469400 39469528 39469400 ID=cds.MS.gene053314.t1;Parent=MS.gene053314.t1
Gene Sequence

>MS.gene053314

ATGGATCCAGGAGCGATGATGAACGAGGGTTCATTTCCGAATGGGAATGGAAATACGACGCCGTTTAGTTTAGCGGAGATCTGGCAGTTTCCAGCGGCGATTAATGGCGGCGGAGGTGGATTAGGACTAAGAAGGCCGCAGTTTGGGAACGGTTTAGGACAGTTTGGTGAGTTTGGACCCGGTTTAAACCGGGATGGTAATGGACCGGAGTCAAGAGTTGTTTTGAACCATGTTGGTGGTGGTAAGAAGAGGCGTGACTCGGAAGATGATTCTGCTAAGTGTGTTTCAACCAGTAATGGCGGTGCCAATGCCGTGGTATTCATTTTTTTCTTCTTTTTTTAGTTTTTTTTTAGTTATATTTGGAAGGAATTCATTGCAGTAACTGTTTGATTAAATAAAGTATAAAGTATAAATTAAATATTAAATATATGTATTTTGGGGAGTGATTAATTGGTTGATTAAGAAAGATGAGTTGTTGTTTTAATGAATATACTTAAAACGGATCTAATTATGTTGTTAATCATTGTTCAACTTGTATTTTTGGATTTTGTATATCTACTAAAAATGTGTTTTTTATTTTATTATTGGAATTTTTTTGCAATTTTCCCGCTCAAGATTTTGCTTCATTTTTTTTTGGTACAAGGAATAAAATAGTGTTGTTATAAATTCTAATTATGTTTTTAACAATTTCACAAACTAATTTGCCTATGGAAGTGTAATCTCAACACTTATTGATTACTATTTCACTTTTGATTTTAAAAATGGTGTGTGAACTTATTCAGGTTTGGTCGCAATTTCAAGGATTTTGATGTCTTTGCTACAACATAAGGACCAAAATTTTGGCTACTTCGCTCGTATTTAAACCCAATTTTCTATCGTTTTGTGACTATGATTGCAATTCAAATTTTTGTTTCGAACTTGGTTGTCCTAATTATTCTGGAGAAAAATTCCTTTATTGTAATAATTTAGTTATCTATATGTGTATTTGATGTATTTGGAATTAGTCGGTGTGATTTAATGGAATTATTATTATTTGGATTGTGCAGAATGATAGTGATGGAAAACGGACTAAAGCATCAGGGAACAAGAATGAGGGTGGTGATGGTAAAGTTGAAGGAGAAGTCAGTTCAGGAAAGCATGCGGAGCAAAGCAGTAAGCCACCTCCTTCGGACCCTCCTAAGCAAGATTACATCCATGTTCGAGCTAGAAGGGGTCAAGCTACTGATAGCCACAGTCTTGCAGAGCGCGTAATTTACCCTTTACTTACAAATCTTTTAGAAACTCTTGTGTTATTGTTTTTAGGTCCTTTTGTCTGTCTAATTGTTAAGATCAATTTCTTAAGCAAGATAACAGCAATTGAGTTGAGAAGCGCCGTTTTGGCTGTGACATCATAGGTTTTGATAATCAGATTATCAGACATATTTAACCGCATTTCTCAACATCAAGAGTTAGGACACAATCGTTGCTATGATGTTAAATCTTGTTTAAGGAAAATGTAAATTTATGGTTTTGTTGCGGTTAGAGTCTATGCCTCTGTTTCTAATTGGAATGTTGGTGGACAATAAACAGGCTAGAAGGGAAAAGATTAGTGAAAGGATGAAAATTCTACAGGATTTAGTTCCTGGTTGTAATAAGGTAATGTTTTTACTACTTGTTAATCTTTTCTGCTCTCATATTCACATAAAAGGAAATGAAATGAAAATAGTAATAATAATAATAATCCAAATGAAAGTAAATTACTAAAAGTAGGAAAAACGTGCTACTTGGGCCCATGAAAAGAAAATAACAGTTATGATCTTCCTAATTGTTGGTCAGTTATAATATCATATTTTCTATGAAATAATAAATTGAGACTTTCTTCAATTTAGGTTATTGGGAAAGCATTGGTGCTTGATGAGATAATTAATTATATTCAATCACTTCAGCGCCAAGTGGAGGTATCTATTCTATTTCTGACTTTGGTATTTACTGAATTATAATCTTTTCAAAATTTATTATGTTTTGAAATTGATTTTTGACTATTGTAATGTTAATTGATGTCACAGTTTTTATCGATGAAACTTGAAGCAGTTAATTCAAGACTCAATACCGGCATTGAAGCCTTTCCTCCAAAAGATGTAAGTGTTAAGCATCCCTTGATTTTTATATTGAAACTCTTATCAAGTTGAAAGCTTGTTTCTACCCTCTTTATTTGAAAATTTTCCTTCTGACAAGACATGGCTCGCATAACTCACGAGTATAGTAGTTCTGGTGATTGCTATGTTGCAAGTATGTATAACAAGGTTGAACCATCTTTTGCCGACATTATTCACGCACAAGATTTTTGTTTCATTTGCAAATTTTGTTGTTGAGTACTCTGCAATTTTTGTTGTTGTTGAGTACTTTGCTTCAAATGAATGATAGCTTCTCGCTAGGTGCTTTTCAGAACTGATTAGTTTAAGAAGCTTGCAACCTCTGGTCTCTGCATAATGGGCGAGAGTATTGAACTCTCAGCCCTTGGATTAATCCAATGTTCAATCTTTAGCTGTAGAGGATAAATATACTTGATTGAGAGGATACAGATTTAAAGTTTGGATTAAAATGATTGATTTAGTTTTTTATTATTCATCATTGTTTCAAAAACATTGGACTTACAAGACCTAGTTTTTTTTTCTCTCTTTCCAGTATGGTCAACAAACGTTTGATCTTGCCGGCATGCCATTTGTTTCACAAGCTACAAGAGAGCCAAGCAGAGGTTCTTCACCAGAATGGTTACATATGCAGGTAGGTGGTGGTTTCGAAAGAACGACGTAG
Protein sequence

>MS.gene053314.t1

MDPGAMMNEGSFPNGNGNTTPFSLAEIWQFPAAINGGGGGLGLRRPQFGNGLGQFGEFGPGLNRDGNGPESRVVLNHVGGGKKRRDSEDDSAKCVSTSNGGANAVNDSDGKRTKASGNKNEGGDGKVEGEVSSGKHAEQSSKPPPSDPPKQDYIHVRARRGQATDSHSLAERARREKISERMKILQDLVPGCNKVIGKALVLDEIINYIQSLQRQVEFLSMKLEAVNSRLNTGIEAFPPKDYGQQTFDLAGMPFVSQATREPSRGSSPEWLHMQVGGGFERTT