AlfalfaGEDB | MS.gene053390

M. sativa cultivar XinJiangDaYe / MS.gene053390

KEGG: K07760
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053390.t1	CAA65982.1	95	80	4	0	1	80	1	80	2.60E-32	147.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053390.t1	Q8LF80	83.1	71	12	0	10	80	6	76	6.3e-27	120.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053390.t1	G7I4T8	95.0	80	4	0	1	80	1	80	1.9e-32	147.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053390.t1	MTR_1g075610	95.000	80	4	0	1	80	1	80	3.07e-46	149
MS.gene053390.t1	MTR_4g007750	67.692	65	21	0	16	80	2	66	1.31e-23	90.9
MS.gene053390.t1	MTR_4g094430	54.237	59	27	0	16	74	2	60	2.74e-18	76.6
MS.gene053390.t1	MTR_2g032060	52.542	59	28	0	16	74	2	60	2.59e-17	73.9
MS.gene053390.t1	MTR_3g040520	55.000	60	27	0	16	75	23	82	1.44e-15	69.7
MS.gene053390.t1	MTR_3g040810	53.333	60	28	0	16	75	21	80	1.88e-15	69.7
MS.gene053390.t1	MTR_4g103810	49.123	57	29	0	18	74	108	164	1.36e-12	61.2
MS.gene053390.t1	MTR_4g109090	47.692	65	33	1	16	80	103	166	2.76e-12	60.5
MS.gene053390.t1	MTR_4g103810	49.123	57	29	0	18	74	218	274	3.18e-12	60.5
MS.gene053390.t1	MTR_2g020210	45.455	77	40	2	5	80	88	163	6.69e-12	59.3
MS.gene053390.t1	MTR_1g098300	42.308	78	43	2	4	80	111	187	2.11e-11	58.2
MS.gene053390.t1	MTR_7g011800	46.753	77	39	2	5	80	106	181	3.11e-11	57.4
MS.gene053390.t1	MTR_8g092290	40.845	71	41	1	10	80	133	202	4.02e-11	57.4
MS.gene053390.t1	MTR_8g099770	47.619	63	32	1	18	80	90	151	4.81e-11	57.0
MS.gene053390.t1	MTR_4g118780	46.154	65	34	1	16	80	102	165	5.26e-11	57.0
MS.gene053390.t1	MTR_6g013030	44.615	65	35	1	16	80	108	171	7.29e-11	56.6
MS.gene053390.t1	MTR_1g081870	44.615	65	35	1	16	80	120	183	8.10e-11	56.2
MS.gene053390.t1	MTR_3g098760	46.154	65	34	1	16	80	95	158	8.24e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053390.t1	AT1G76540	83.099	71	12	0	10	80	6	76	1.03e-35	122
MS.gene053390.t1	AT1G20930	84.058	69	11	0	12	80	10	78	2.01e-34	119
MS.gene053390.t1	AT2G38620	66.154	65	22	0	16	80	2	66	9.20e-24	90.5
MS.gene053390.t1	AT2G38620	66.154	65	22	0	16	80	2	66	9.20e-24	90.5
MS.gene053390.t1	AT2G38620	66.154	65	22	0	16	80	2	66	2.10e-23	90.5
MS.gene053390.t1	AT3G54180	67.692	65	21	0	16	80	2	66	3.34e-23	90.1
MS.gene053390.t1	AT3G48750	52.542	59	28	0	16	74	2	60	4.54e-17	73.2
MS.gene053390.t1	AT5G10270	53.333	60	28	0	16	75	24	83	7.76e-16	70.9
MS.gene053390.t1	AT5G64960	53.333	60	28	0	16	75	24	83	8.32e-16	70.5
MS.gene053390.t1	AT1G67580	50.000	64	31	1	16	79	404	466	1.07e-13	64.7
MS.gene053390.t1	AT1G67580	50.000	64	31	1	16	79	404	466	1.07e-13	64.7
MS.gene053390.t1	AT2G38620	73.171	41	11	0	16	56	2	42	7.46e-13	61.6
MS.gene053390.t1	AT4G10010	49.231	65	32	1	16	80	154	217	9.11e-13	62.0
MS.gene053390.t1	AT1G18670	49.231	65	32	1	16	80	129	192	1.00e-12	62.0
MS.gene053390.t1	AT1G18670	49.231	65	32	1	16	80	129	192	1.33e-12	61.6
MS.gene053390.t1	AT1G18670	49.231	65	32	1	16	80	129	192	1.35e-12	61.6
MS.gene053390.t1	AT1G71530	46.753	77	39	2	5	80	133	208	1.40e-12	61.2
MS.gene053390.t1	AT1G71530	46.753	77	39	2	5	80	133	208	1.40e-12	61.2
MS.gene053390.t1	AT1G71530	46.753	77	39	2	5	80	133	208	1.99e-12	60.8
MS.gene053390.t1	AT1G66750	43.038	79	39	2	1	79	1	73	2.19e-12	60.8
MS.gene053390.t1	AT1G33770	47.692	65	33	1	16	80	139	202	3.56e-12	60.5
MS.gene053390.t1	AT1G71530	49.231	65	32	1	16	80	145	208	5.17e-12	59.7
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	163	227	8.17e-12	59.3
MS.gene053390.t1	AT5G39420	49.231	65	32	1	16	80	103	166	8.59e-12	59.3
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	163	227	9.22e-12	58.9
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	163	227	9.22e-12	58.9
MS.gene053390.t1	AT3G05050	41.558	77	43	2	5	80	124	199	9.81e-12	58.9
MS.gene053390.t1	AT3G05050	41.558	77	43	2	5	80	124	199	9.81e-12	58.9
MS.gene053390.t1	AT3G05050	41.558	77	43	2	5	80	124	199	9.81e-12	58.9
MS.gene053390.t1	AT1G74330	47.692	65	33	1	16	80	119	182	2.59e-11	57.8
MS.gene053390.t1	AT1G74330	47.692	65	33	1	16	80	119	182	2.60e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.70e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.70e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.71e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.71e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.71e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.71e-11	57.8
MS.gene053390.t1	AT5G63370	48.485	66	29	2	18	79	297	361	2.71e-11	57.8
MS.gene053390.t1	AT1G74330	47.692	65	33	1	16	80	119	182	2.87e-11	57.8
MS.gene053390.t1	AT1G57700	46.269	67	35	1	14	80	142	207	4.90e-11	57.0
MS.gene053390.t1	AT1G03740	47.619	63	32	1	18	80	213	274	8.31e-11	56.2
MS.gene053390.t1	AT1G73690	39.241	79	40	2	1	79	1	71	8.57e-11	56.2

Find 18 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTACTGCGATTGTTTCAC+CGG	0.398610	1.4:+27161354	None:intergenic
AACAATCGCAGTAGTGTTAT+CGG	0.409220	1.4:-27161347	MS.gene053390:CDS
CTCATGATCCACATGTTGTT+AGG	0.436119	1.4:-27161147	MS.gene053390:CDS
AGAGCAAGAGAGAAAGCTAC+TGG	0.475204	1.4:-27161265	MS.gene053390:CDS
ACCTCGCGAAGCGTAGTATG+AGG	0.477444	1.4:+27161190	None:intergenic
TTCAAGAAGCTCGAGAAAGT+TGG	0.493220	1.4:-27161313	MS.gene053390:CDS
AAAGTTGGAGGAGGAACGTA+CGG	0.523551	1.4:-27161298	MS.gene053390:CDS
CGCAGTAGTGTTATCGGCGA+AGG	0.529473	1.4:-27161341	MS.gene053390:CDS
TCCTCATACTACGCTTCGCG+AGG	0.556697	1.4:-27161191	MS.gene053390:CDS
AAGAAGCTCGAGAAAGTTGG+AGG	0.564847	1.4:-27161310	MS.gene053390:CDS
CGTCTTCATGAAGATGATGA+AGG	0.573237	1.4:-27161217	MS.gene053390:CDS
TTAACTTACCTAACAACATG+TGG	0.608876	1.4:+27161139	None:intergenic
AAGCTCGAGAAAGTTGGAGG+AGG	0.618189	1.4:-27161307	MS.gene053390:CDS
GAGCAAGAGAGAAAGCTACT+GGG	0.629277	1.4:-27161264	MS.gene053390:CDS
AAGTTGGAGGAGGAACGTAC+GGG	0.630808	1.4:-27161297	MS.gene053390:CDS
ACTACTGCGATTGTTTCACC+GGG	0.640436	1.4:+27161355	None:intergenic
TGGAGGAGGAACGTACGGGA+AGG	0.646655	1.4:-27161293	MS.gene053390:CDS
GATTCAATCATGGAGAAACC+CGG	0.652544	1.4:-27161373	None:intergenic

CRISPR-GE

sgRNA_sequence	Strand	Position	Region	GC_content
AACAATCGCAGTAGTGTTAT+CGG	-	chr1.4:27161161-27161180	MS.gene053390:CDS	35.0%
CGTCTTCATGAAGATGATGA+AGG	-	chr1.4:27161291-27161310	MS.gene053390:CDS	40.0%
CTCATGATCCACATGTTGTT+AGG	-	chr1.4:27161361-27161380	MS.gene053390:CDS	40.0%
TTCAAGAAGCTCGAGAAAGT+TGG	-	chr1.4:27161195-27161214	MS.gene053390:CDS	40.0%
AAAGTTGGAGGAGGAACGTA+CGG	-	chr1.4:27161210-27161229	MS.gene053390:CDS	45.0%
AAGAAGCTCGAGAAAGTTGG+AGG	-	chr1.4:27161198-27161217	MS.gene053390:CDS	45.0%
ACTACTGCGATTGTTTCACC+GGG	+	chr1.4:27161156-27161175	None:intergenic	45.0%
AGAGCAAGAGAGAAAGCTAC+TGG	-	chr1.4:27161243-27161262	MS.gene053390:CDS	45.0%
CACTACTGCGATTGTTTCAC+CGG	+	chr1.4:27161157-27161176	None:intergenic	45.0%
GAGCAAGAGAGAAAGCTACT+GGG	-	chr1.4:27161244-27161263	MS.gene053390:CDS	45.0%
AAGCTCGAGAAAGTTGGAGG+AGG	-	chr1.4:27161201-27161220	MS.gene053390:CDS	50.0%
AAGTTGGAGGAGGAACGTAC+GGG	-	chr1.4:27161211-27161230	MS.gene053390:CDS	50.0%
ACCTCGCGAAGCGTAGTATG+AGG	+	chr1.4:27161321-27161340	None:intergenic	55.0%
CGCAGTAGTGTTATCGGCGA+AGG	-	chr1.4:27161167-27161186	MS.gene053390:CDS	55.0%
TCCTCATACTACGCTTCGCG+AGG	-	chr1.4:27161317-27161336	MS.gene053390:CDS	55.0%
TGGAGGAGGAACGTACGGGA+AGG	-	chr1.4:27161215-27161234	MS.gene053390:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	27161144	27161386	27161144	ID=MS.gene053390
chr1.4	mRNA	27161144	27161386	27161144	ID=MS.gene053390.t1;Parent=MS.gene053390
chr1.4	exon	27161144	27161386	27161144	ID=MS.gene053390.t1.exon1;Parent=MS.gene053390.t1
chr1.4	CDS	27161144	27161386	27161144	ID=cds.MS.gene053390.t1;Parent=MS.gene053390.t1

Gene Sequence	>MS.gene053390 ATGGAGAAACCCGGTGAAACAATCGCAGTAGTGTTATCGGCGAAGGAAGCATTCAAGAAGCTCGAGAAAGTTGGAGGAGGAACGTACGGGAAGGTTTACAGAGCAAGAGAGAAAGCTACTGGGAAGATTGTTGCGTTGAAGAAGACTCGTCTTCATGAAGATGATGAAGGTGTTCCTCATACTACGCTTCGCGAGGTTTCGATTTTGCGTATGCTCTCTCATGATCCACATGTTGTTAGGTAA
Protein sequence	>MS.gene053390.t1 MEKPGETIAVVLSAKEAFKKLEKVGGGTYGKVYRAREKATGKIVALKKTRLHEDDEGVPHTTLREVSILRMLSHDPHVVR