Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053453.t1 | AES60398.2 | 85.8 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 1.20E-58 | 235.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053453.t1 | Q5PP38 | 32.2 | 121 | 72 | 3 | 3 | 115 | 12 | 130 | 1.1e-07 | 57.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053453.t1 | G7I6L4 | 85.8 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 8.8e-59 | 235.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene053453.t1 | TF | C2C2-GATA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053453.t1 | MTR_1g045040 | 85.821 | 134 | 19 | 0 | 1 | 134 | 1 | 134 | 7.82e-76 | 221 |
| MS.gene053453.t1 | MTR_1g089830 | 75.573 | 131 | 25 | 2 | 1 | 130 | 1 | 125 | 5.38e-67 | 199 |
| MS.gene053453.t1 | MTR_8g089130 | 35.762 | 151 | 71 | 3 | 6 | 130 | 7 | 157 | 3.32e-21 | 83.6 |
| MS.gene053453.t1 | MTR_8g089170 | 39.189 | 148 | 62 | 4 | 6 | 127 | 7 | 152 | 4.05e-21 | 83.6 |
| MS.gene053453.t1 | MTR_8g039350 | 73.171 | 41 | 11 | 0 | 4 | 44 | 15 | 55 | 5.74e-17 | 70.5 |
| MS.gene053453.t1 | MTR_4g028990 | 41.667 | 96 | 46 | 4 | 43 | 130 | 3 | 96 | 6.42e-13 | 60.8 |
| MS.gene053453.t1 | MTR_5g013370 | 51.852 | 54 | 25 | 1 | 3 | 56 | 12 | 64 | 2.78e-11 | 60.1 |
| MS.gene053453.t1 | MTR_5g013370 | 51.852 | 54 | 25 | 1 | 3 | 56 | 12 | 64 | 3.76e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAAAAGCTTCAAAGTCTA+TGG | 0.184389 | 1.4:+29364991 | MS.gene053453:CDS |
| GAGCTTTCAAACCAATTTCC+AGG | 0.378813 | 1.4:+29364674 | MS.gene053453:CDS |
| TGGAAATTGGTTTGAAAGCT+CGG | 0.417197 | 1.4:-29364672 | None:intergenic |
| CCACAAGCATTACATAGTAT+TGG | 0.433061 | 1.4:-29364545 | None:intergenic |
| GAAACTAAACTTTGGCGTAA+AGG | 0.456777 | 1.4:+29364509 | MS.gene053453:CDS |
| TGCTTGTGGACTACATTTCA+AGG | 0.461822 | 1.4:+29364559 | MS.gene053453:CDS |
| ATCAGACCTTGACGTTGAAA+AGG | 0.465983 | 1.4:+29364634 | MS.gene053453:CDS |
| GGACTACATTTCAAGGCTAA+AGG | 0.474721 | 1.4:+29364566 | MS.gene053453:CDS |
| TCTTCGGAAGATAGTTATCA+AGG | 0.478433 | 1.4:-29364592 | None:intergenic |
| TGTATACCAAGCAATGAGAT+AGG | 0.478624 | 1.4:+29365080 | MS.gene053453:CDS |
| GGAAATTGGTTTGAAAGCTC+GGG | 0.487200 | 1.4:-29364671 | None:intergenic |
| ATCGATTGTGAAGAATCAAA+TGG | 0.491491 | 1.4:+29364959 | MS.gene053453:CDS |
| CATAGTATTGGCTTCTCATA+CGG | 0.492997 | 1.4:-29364533 | None:intergenic |
| CCAAGCAATGAGATAGGCAT+TGG | 0.496933 | 1.4:+29365086 | MS.gene053453:CDS |
| ATTTGATTCTTCACAATCGA+TGG | 0.523804 | 1.4:-29364957 | None:intergenic |
| ACAAAAGCTTCAAAGTCTAT+GGG | 0.556384 | 1.4:+29364992 | MS.gene053453:CDS |
| CCAATGCCTATCTCATTGCT+TGG | 0.566560 | 1.4:-29365086 | None:intergenic |
| CCAATACTATGTAATGCTTG+TGG | 0.579274 | 1.4:+29364545 | MS.gene053453:CDS |
| CTTCGGAAGATAGTTATCAA+GGG | 0.629882 | 1.4:-29364591 | None:intergenic |
| GATTGTGAAGAATCAAATGG+AGG | 0.680011 | 1.4:+29364962 | MS.gene053453:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTTTAATTTTTTGTAGAA+AGG | + | chr1.4:29364931-29364950 | MS.gene053453:intron | 15.0% |
| !!! | CTTTATATTTGTTGAAAAAT+GGG | - | chr1.4:29364733-29364752 | None:intergenic | 15.0% |
| !!! | TTTATATTTGTTGAAAAATG+GGG | - | chr1.4:29364732-29364751 | None:intergenic | 15.0% |
| !! | TTATATGTATACATCATACA+CGG | - | chr1.4:29364911-29364930 | None:intergenic | 20.0% |
| !!! | ATTGTAAGAATTTTCTTAAG+AGG | + | chr1.4:29364787-29364806 | MS.gene053453:intron | 20.0% |
| !!! | ATTTTTTATTACCTGGAAAT+TGG | - | chr1.4:29364688-29364707 | None:intergenic | 20.0% |
| !!! | GCTTTATATTTGTTGAAAAA+TGG | - | chr1.4:29364734-29364753 | None:intergenic | 20.0% |
| !!! | TTATTGCATTTTTTATTACC+TGG | - | chr1.4:29364695-29364714 | None:intergenic | 20.0% |
| ! | TATCCATAAATGTATGAATG+TGG | - | chr1.4:29364820-29364839 | None:intergenic | 25.0% |
| !! | TTTTGACAGAAACTAAACTT+TGG | + | chr1.4:29364501-29364520 | MS.gene053453:CDS | 25.0% |
| AACAAAAGCTTCAAAGTCTA+TGG | + | chr1.4:29364991-29365010 | MS.gene053453:CDS | 30.0% | |
| ACAAAAGCTTCAAAGTCTAT+GGG | + | chr1.4:29364992-29365011 | MS.gene053453:CDS | 30.0% | |
| ACTCCACATTCATACATTTA+TGG | + | chr1.4:29364814-29364833 | MS.gene053453:intron | 30.0% | |
| ATCGATTGTGAAGAATCAAA+TGG | + | chr1.4:29364959-29364978 | MS.gene053453:CDS | 30.0% | |
| ATTTGATTCTTCACAATCGA+TGG | - | chr1.4:29364960-29364979 | None:intergenic | 30.0% | |
| !! | GTCTGATAACTGATTTTGTT+GGG | - | chr1.4:29364621-29364640 | None:intergenic | 30.0% |
| CCAATACTATGTAATGCTTG+TGG | + | chr1.4:29364545-29364564 | MS.gene053453:CDS | 35.0% | |
| CCACAAGCATTACATAGTAT+TGG | - | chr1.4:29364548-29364567 | None:intergenic | 35.0% | |
| CTTCGGAAGATAGTTATCAA+GGG | - | chr1.4:29364594-29364613 | None:intergenic | 35.0% | |
| GAAACTAAACTTTGGCGTAA+AGG | + | chr1.4:29364509-29364528 | MS.gene053453:CDS | 35.0% | |
| GATTGTGAAGAATCAAATGG+AGG | + | chr1.4:29364962-29364981 | MS.gene053453:CDS | 35.0% | |
| TCATACACGGTACATATCAA+TGG | - | chr1.4:29364898-29364917 | None:intergenic | 35.0% | |
| TCTTCGGAAGATAGTTATCA+AGG | - | chr1.4:29364595-29364614 | None:intergenic | 35.0% | |
| TGTATACCAAGCAATGAGAT+AGG | + | chr1.4:29365080-29365099 | MS.gene053453:CDS | 35.0% | |
| ! | TGATTTTGTTGGGATGTCTT+CGG | - | chr1.4:29364611-29364630 | None:intergenic | 35.0% |
| !! | CATAGTATTGGCTTCTCATA+CGG | - | chr1.4:29364536-29364555 | None:intergenic | 35.0% |
| !! | GGTCTGATAACTGATTTTGT+TGG | - | chr1.4:29364622-29364641 | None:intergenic | 35.0% |
| !! | TGGAAATTGGTTTGAAAGCT+CGG | - | chr1.4:29364675-29364694 | None:intergenic | 35.0% |
| ATCAGACCTTGACGTTGAAA+AGG | + | chr1.4:29364634-29364653 | MS.gene053453:CDS | 40.0% | |
| GAGCTTTCAAACCAATTTCC+AGG | + | chr1.4:29364674-29364693 | MS.gene053453:CDS | 40.0% | |
| GGACTACATTTCAAGGCTAA+AGG | + | chr1.4:29364566-29364585 | MS.gene053453:CDS | 40.0% | |
| ! | CAGATTCCTTTTCAACGTCA+AGG | - | chr1.4:29364643-29364662 | None:intergenic | 40.0% |
| ! | TGCTTGTGGACTACATTTCA+AGG | + | chr1.4:29364559-29364578 | MS.gene053453:CDS | 40.0% |
| !! | GGAAATTGGTTTGAAAGCTC+GGG | - | chr1.4:29364674-29364693 | None:intergenic | 40.0% |
| CCAAGCAATGAGATAGGCAT+TGG | + | chr1.4:29365086-29365105 | MS.gene053453:CDS | 45.0% | |
| CCAATGCCTATCTCATTGCT+TGG | - | chr1.4:29365089-29365108 | None:intergenic | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 29364497 | 29365154 | 29364497 | ID=MS.gene053453 |
| chr1.4 | mRNA | 29364497 | 29365154 | 29364497 | ID=MS.gene053453.t1;Parent=MS.gene053453 |
| chr1.4 | exon | 29364497 | 29364695 | 29364497 | ID=MS.gene053453.t1.exon1;Parent=MS.gene053453.t1 |
| chr1.4 | CDS | 29364497 | 29364695 | 29364497 | ID=cds.MS.gene053453.t1;Parent=MS.gene053453.t1 |
| chr1.4 | exon | 29364949 | 29365154 | 29364949 | ID=MS.gene053453.t1.exon2;Parent=MS.gene053453.t1 |
| chr1.4 | CDS | 29364949 | 29365154 | 29364949 | ID=cds.MS.gene053453.t1;Parent=MS.gene053453.t1 |
| Gene Sequence |
| Protein sequence |
