Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053465.t1 | XP_013461367.1 | 88.7 | 53 | 6 | 0 | 1 | 53 | 1 | 53 | 5.20E-17 | 97.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053465.t1 | Q6R0C4 | 67.5 | 40 | 13 | 0 | 14 | 53 | 3 | 42 | 3.4e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053465.t1 | A0A072V0T7 | 88.7 | 53 | 6 | 0 | 1 | 53 | 1 | 53 | 3.8e-17 | 97.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene053465.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053465.t1 | MTR_3g089045 | 83.929 | 56 | 9 | 0 | 1 | 56 | 1 | 56 | 6.78e-27 | 101 |
| MS.gene053465.t1 | MTR_6g074860 | 60.465 | 43 | 17 | 0 | 14 | 56 | 2 | 44 | 2.19e-12 | 62.4 |
| MS.gene053465.t1 | MTR_4g082040 | 60.870 | 46 | 18 | 0 | 11 | 56 | 100 | 145 | 3.49e-12 | 62.4 |
| MS.gene053465.t1 | MTR_7g086960 | 53.571 | 56 | 23 | 1 | 1 | 53 | 71 | 126 | 5.36e-12 | 62.0 |
| MS.gene053465.t1 | MTR_5g041570 | 58.696 | 46 | 19 | 0 | 11 | 56 | 129 | 174 | 1.23e-11 | 60.8 |
| MS.gene053465.t1 | MTR_4g117140 | 93.103 | 29 | 2 | 0 | 101 | 129 | 398 | 426 | 9.41e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053465.t1 | AT1G17950 | 62.791 | 43 | 16 | 0 | 14 | 56 | 3 | 45 | 4.29e-13 | 64.3 |
| MS.gene053465.t1 | AT1G73410 | 70.000 | 40 | 12 | 0 | 14 | 53 | 4 | 43 | 1.93e-12 | 62.0 |
| MS.gene053465.t1 | AT1G73410 | 65.116 | 43 | 15 | 0 | 14 | 56 | 4 | 46 | 2.31e-12 | 62.4 |
| MS.gene053465.t1 | AT4G33450 | 50.877 | 57 | 27 | 1 | 1 | 56 | 3 | 59 | 4.98e-12 | 61.2 |
| MS.gene053465.t1 | AT1G69560 | 63.636 | 44 | 16 | 0 | 13 | 56 | 120 | 163 | 3.19e-11 | 59.7 |
| MS.gene053465.t1 | AT1G69560 | 63.636 | 44 | 16 | 0 | 13 | 56 | 104 | 147 | 3.49e-11 | 59.7 |
Find 45 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGTATGGTGCTCAAAAT+TGG | 0.302805 | 1.4:-29506904 | MS.gene053465:CDS |
| GTATGCAAGGCCACCTCTTT+AGG | 0.329420 | 1.4:+29506182 | None:intergenic |
| TTACCACCCATTTGGTTCAT+TGG | 0.340765 | 1.4:+29506068 | None:intergenic |
| CATCAACTTGTTGAACAGTA+TGG | 0.349125 | 1.4:-29506917 | MS.gene053465:CDS |
| TAAGGATCTGGAGGAGGATA+AGG | 0.379837 | 1.4:-29506228 | MS.gene053465:CDS |
| CGGGAATGTTACCACCCATT+TGG | 0.397076 | 1.4:+29506060 | None:intergenic |
| CACTGAAAGCAGTAAGGATC+TGG | 0.421437 | 1.4:-29506240 | MS.gene053465:CDS |
| TGGTTCATTGGCATACTCAT+TGG | 0.433991 | 1.4:+29506080 | None:intergenic |
| AATGGCATACTCAGCGGGCC+TGG | 0.436759 | 1.4:+29506128 | None:intergenic |
| AGGCCCGCTGAGTATGCCAT+TGG | 0.442285 | 1.4:-29506126 | MS.gene053465:CDS |
| GGTAACATTCCCGCTGTTCA+AGG | 0.449548 | 1.4:-29506050 | MS.gene053465:CDS |
| AATGCATACCTGATATTCCT+TGG | 0.469302 | 1.4:+29506861 | None:intergenic |
| GGTCTACCGGTAGGCGGACG+AGG | 0.486166 | 1.4:-29506029 | MS.gene053465:CDS |
| AATTATCCTCGTCCGCCTAC+CGG | 0.487010 | 1.4:+29506023 | None:intergenic |
| AAGGATCTGGAGGAGGATAA+GGG | 0.496725 | 1.4:-29506227 | MS.gene053465:CDS |
| AGAGTTGTCCACGTGGTCAC+TGG | 0.498220 | 1.4:-29506964 | MS.gene053465:CDS |
| GGTGGCCTTGCATACATCAT+TGG | 0.501791 | 1.4:-29506174 | MS.gene053465:CDS |
| TCTGGAGGAGGATAAGGGTA+AGG | 0.506412 | 1.4:-29506222 | MS.gene053465:CDS |
| GAGTATGCCAATGAACCAAA+TGG | 0.517292 | 1.4:-29506075 | MS.gene053465:CDS |
| TACATGTAATTCACCTAAAG+AGG | 0.517912 | 1.4:-29506195 | MS.gene053465:CDS |
| CACCCAATGGCATACTCAGC+GGG | 0.518715 | 1.4:+29506123 | None:intergenic |
| GGCCCGCTGAGTATGCCATT+GGG | 0.519063 | 1.4:-29506125 | MS.gene053465:CDS |
| ACCGGTAGACCTTGAACAGC+GGG | 0.524759 | 1.4:+29506041 | None:intergenic |
| GTGACCACGTGGACAACTCT+TGG | 0.531230 | 1.4:+29506967 | None:intergenic |
| CTGAGTATGCCATTGGGTGG+TGG | 0.538925 | 1.4:-29506119 | MS.gene053465:CDS |
| TCCCGCTGTTCAAGGTCTAC+CGG | 0.542315 | 1.4:-29506042 | MS.gene053465:CDS |
| GGAGGAGGATAAGGGTAAGG+AGG | 0.547967 | 1.4:-29506219 | MS.gene053465:CDS |
| AGTATGCCATTGGGTGGTGG+AGG | 0.566357 | 1.4:-29506116 | MS.gene053465:CDS |
| TCTCATCACTGAAAGCAGTA+AGG | 0.568940 | 1.4:-29506246 | MS.gene053465:CDS |
| AGCAGCCAATGATGTATGCA+AGG | 0.586173 | 1.4:+29506169 | None:intergenic |
| AGTATGCCAATGAACCAAAT+GGG | 0.610690 | 1.4:-29506074 | MS.gene053465:CDS |
| AAGCAGTAAGGATCTGGAGG+AGG | 0.611376 | 1.4:-29506234 | MS.gene053465:CDS |
| GCTGTTCAAGGTCTACCGGT+AGG | 0.626077 | 1.4:-29506038 | MS.gene053465:CDS |
| TGAAAGCAGTAAGGATCTGG+AGG | 0.633875 | 1.4:-29506237 | MS.gene053465:CDS |
| ATGTAATTCACCTAAAGAGG+TGG | 0.643426 | 1.4:-29506192 | MS.gene053465:CDS |
| TTATTACCTCCACCACCCAA+TGG | 0.644634 | 1.4:+29506110 | None:intergenic |
| TACCGGTAGACCTTGAACAG+CGG | 0.649561 | 1.4:+29506040 | None:intergenic |
| GTTCAAGGTCTACCGGTAGG+CGG | 0.653590 | 1.4:-29506035 | MS.gene053465:CDS |
| CCGCTGAGTATGCCATTGGG+TGG | 0.668665 | 1.4:-29506122 | MS.gene053465:CDS |
| GGTGGTGGAGGTAATAATGT+GGG | 0.672000 | 1.4:-29506104 | MS.gene053465:CDS |
| CCACCCAATGGCATACTCAG+CGG | 0.676473 | 1.4:+29506122 | None:intergenic |
| GGGTGGTGGAGGTAATAATG+TGG | 0.682196 | 1.4:-29506105 | MS.gene053465:CDS |
| ATGCCAATGAACCAAATGGG+TGG | 0.690349 | 1.4:-29506071 | MS.gene053465:CDS |
| GCGGGTCGCCAGTGACCACG+TGG | 0.707521 | 1.4:+29506956 | None:intergenic |
| GATACCAAGAGTTGTCCACG+TGG | 0.762573 | 1.4:-29506971 | MS.gene053465:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGAATTTGAAAAATTAAATA+AGG | + | chr1.4:29506234-29506253 | None:intergenic | 10.0% |
| !!! | TATTTAATTTTTCAAATTCA+TGG | - | chr1.4:29506234-29506253 | MS.gene053465:CDS | 10.0% |
| !!! | TTTATATGTGTTTATATTTT+GGG | + | chr1.4:29506468-29506487 | None:intergenic | 10.0% |
| !!! | ACTATGTTTATTTTTTTCTT+TGG | + | chr1.4:29506518-29506537 | None:intergenic | 15.0% |
| !!! | GTTTATATGTGTTTATATTT+TGG | + | chr1.4:29506469-29506488 | None:intergenic | 15.0% |
| !! | AAAGTAATGTTATTGAGAAT+GGG | + | chr1.4:29506419-29506438 | None:intergenic | 20.0% |
| !! | ATATTGCATAAAATTAGAGA+GGG | - | chr1.4:29506668-29506687 | MS.gene053465:intron | 20.0% |
| !! | TAAAAAACTCATGTTTCATT+GGG | - | chr1.4:29506639-29506658 | MS.gene053465:intron | 20.0% |
| !! | TATATTGCATAAAATTAGAG+AGG | - | chr1.4:29506667-29506686 | MS.gene053465:intron | 20.0% |
| !! | TTGAAACTTTAATATTGCTA+TGG | - | chr1.4:29506381-29506400 | MS.gene053465:intron | 20.0% |
| ! | CTAAAAAACTCATGTTTCAT+TGG | - | chr1.4:29506638-29506657 | MS.gene053465:intron | 25.0% |
| ! | GAAAGTAATGTTATTGAGAA+TGG | + | chr1.4:29506420-29506439 | None:intergenic | 25.0% |
| !! | ATATAGTTGATTAGAAGTGT+TGG | - | chr1.4:29506265-29506284 | MS.gene053465:intron | 25.0% |
| !!! | GAAATCTTTGATAATTTTCC+AGG | - | chr1.4:29506877-29506896 | MS.gene053465:CDS | 25.0% |
| !!! | TATAGTTGATTAGAAGTGTT+GGG | - | chr1.4:29506266-29506285 | MS.gene053465:intron | 25.0% |
| TACATGTAATTCACCTAAAG+AGG | - | chr1.4:29506828-29506847 | MS.gene053465:intron | 30.0% | |
| TGATTCATCAAAACCAACAA+TGG | + | chr1.4:29506729-29506748 | None:intergenic | 30.0% | |
| TTCTATAAACTACTCCTCTA+TGG | - | chr1.4:29506555-29506574 | MS.gene053465:intron | 30.0% | |
| ! | ATGAAGTTTTTCATCTTCAG+CGG | + | chr1.4:29506089-29506108 | None:intergenic | 30.0% |
| AACAGTATGGTGCTCAAAAT+TGG | - | chr1.4:29506119-29506138 | MS.gene053465:CDS | 35.0% | |
| AATGCATACCTGATATTCCT+TGG | + | chr1.4:29506165-29506184 | None:intergenic | 35.0% | |
| ACATGACATAATATTGCAGC+TGG | - | chr1.4:29506748-29506767 | MS.gene053465:intron | 35.0% | |
| AGTATGCCAATGAACCAAAT+GGG | - | chr1.4:29506949-29506968 | MS.gene053465:CDS | 35.0% | |
| ATCAAAACCAACAATGGTCA+TGG | + | chr1.4:29506723-29506742 | None:intergenic | 35.0% | |
| ATGTAATTCACCTAAAGAGG+TGG | - | chr1.4:29506831-29506850 | MS.gene053465:intron | 35.0% | |
| GAAAAACTCCAAGGAATATC+AGG | - | chr1.4:29506154-29506173 | MS.gene053465:CDS | 35.0% | |
| ! | TGAAGTTTTTCATCTTCAGC+GGG | + | chr1.4:29506088-29506107 | None:intergenic | 35.0% |
| !! | CATCAACTTGTTGAACAGTA+TGG | - | chr1.4:29506106-29506125 | MS.gene053465:CDS | 35.0% |
| AAACCCCCTAAAGATGAAAC+GGG | - | chr1.4:29506585-29506604 | MS.gene053465:intron | 40.0% | |
| GAAACCCCCTAAAGATGAAA+CGG | - | chr1.4:29506584-29506603 | MS.gene053465:intron | 40.0% | |
| GAGTATGCCAATGAACCAAA+TGG | - | chr1.4:29506948-29506967 | MS.gene053465:CDS | 40.0% | |
| GGGGTTTCTAATTACCATAG+AGG | + | chr1.4:29506572-29506591 | None:intergenic | 40.0% | |
| TCAACTGCTGAAAAACTCCA+AGG | - | chr1.4:29506145-29506164 | MS.gene053465:CDS | 40.0% | |
| TCTCATCACTGAAAGCAGTA+AGG | - | chr1.4:29506777-29506796 | MS.gene053465:intron | 40.0% | |
| TGATCCCCGTTTCATCTTTA+GGG | + | chr1.4:29506593-29506612 | None:intergenic | 40.0% | |
| TGGTTCATTGGCATACTCAT+TGG | + | chr1.4:29506946-29506965 | None:intergenic | 40.0% | |
| TTGATCCCCGTTTCATCTTT+AGG | + | chr1.4:29506594-29506613 | None:intergenic | 40.0% | |
| ! | TTACCACCCATTTGGTTCAT+TGG | + | chr1.4:29506958-29506977 | None:intergenic | 40.0% |
| AACCCCCTAAAGATGAAACG+GGG | - | chr1.4:29506586-29506605 | MS.gene053465:intron | 45.0% | |
| AAGGATCTGGAGGAGGATAA+GGG | - | chr1.4:29506796-29506815 | MS.gene053465:intron | 45.0% | |
| ACCAACAATGGTCATGGTCA+TGG | + | chr1.4:29506717-29506736 | None:intergenic | 45.0% | |
| ACCATGACCATGACCATTGT+TGG | - | chr1.4:29506713-29506732 | MS.gene053465:intron | 45.0% | |
| AGCAGCCAATGATGTATGCA+AGG | + | chr1.4:29506857-29506876 | None:intergenic | 45.0% | |
| ATCCCCGTTTCATCTTTAGG+GGG | + | chr1.4:29506591-29506610 | None:intergenic | 45.0% | |
| ATGCCAATGAACCAAATGGG+TGG | - | chr1.4:29506952-29506971 | MS.gene053465:CDS | 45.0% | |
| CACTGAAAGCAGTAAGGATC+TGG | - | chr1.4:29506783-29506802 | MS.gene053465:intron | 45.0% | |
| GATCCCCGTTTCATCTTTAG+GGG | + | chr1.4:29506592-29506611 | None:intergenic | 45.0% | |
| TAAGGATCTGGAGGAGGATA+AGG | - | chr1.4:29506795-29506814 | MS.gene053465:intron | 45.0% | |
| TGAAAGCAGTAAGGATCTGG+AGG | - | chr1.4:29506786-29506805 | MS.gene053465:intron | 45.0% | |
| TTATTACCTCCACCACCCAA+TGG | + | chr1.4:29506916-29506935 | None:intergenic | 45.0% | |
| !! | GGTGGTGGAGGTAATAATGT+GGG | - | chr1.4:29506919-29506938 | MS.gene053465:CDS | 45.0% |
| AAGCAGTAAGGATCTGGAGG+AGG | - | chr1.4:29506789-29506808 | MS.gene053465:intron | 50.0% | |
| CGGGAATGTTACCACCCATT+TGG | + | chr1.4:29506966-29506985 | None:intergenic | 50.0% | |
| GATACCAAGAGTTGTCCACG+TGG | - | chr1.4:29506052-29506071 | MS.gene053465:CDS | 50.0% | |
| GGTAACATTCCCGCTGTTCA+AGG | - | chr1.4:29506973-29506992 | MS.gene053465:CDS | 50.0% | |
| GTATGCAAGGCCACCTCTTT+AGG | + | chr1.4:29506844-29506863 | None:intergenic | 50.0% | |
| TACCGGTAGACCTTGAACAG+CGG | + | chr1.4:29506986-29507005 | None:intergenic | 50.0% | |
| TCTGGAGGAGGATAAGGGTA+AGG | - | chr1.4:29506801-29506820 | MS.gene053465:intron | 50.0% | |
| ! | GGTGGCCTTGCATACATCAT+TGG | - | chr1.4:29506849-29506868 | MS.gene053465:intron | 50.0% |
| !! | GGGTGGTGGAGGTAATAATG+TGG | - | chr1.4:29506918-29506937 | MS.gene053465:CDS | 50.0% |
| ACCGGTAGACCTTGAACAGC+GGG | + | chr1.4:29506985-29507004 | None:intergenic | 55.0% | |
| AGAGTTGTCCACGTGGTCAC+TGG | - | chr1.4:29506059-29506078 | MS.gene053465:CDS | 55.0% | |
| CACCCAATGGCATACTCAGC+GGG | + | chr1.4:29506903-29506922 | None:intergenic | 55.0% | |
| CCACCCAATGGCATACTCAG+CGG | + | chr1.4:29506904-29506923 | None:intergenic | 55.0% | |
| CTGAGTATGCCATTGGGTGG+TGG | - | chr1.4:29506904-29506923 | MS.gene053465:CDS | 55.0% | |
| GCTGTTCAAGGTCTACCGGT+AGG | - | chr1.4:29506985-29507004 | MS.gene053465:CDS | 55.0% | |
| GGAGGAGGATAAGGGTAAGG+AGG | - | chr1.4:29506804-29506823 | MS.gene053465:intron | 55.0% | |
| GTGACCACGTGGACAACTCT+TGG | + | chr1.4:29506059-29506078 | None:intergenic | 55.0% | |
| GTTCAAGGTCTACCGGTAGG+CGG | - | chr1.4:29506988-29507007 | MS.gene053465:CDS | 55.0% | |
| TCCCGCTGTTCAAGGTCTAC+CGG | - | chr1.4:29506981-29507000 | MS.gene053465:CDS | 55.0% | |
| ! | AGTATGCCATTGGGTGGTGG+AGG | - | chr1.4:29506907-29506926 | MS.gene053465:CDS | 55.0% |
| AATGGCATACTCAGCGGGCC+TGG | + | chr1.4:29506898-29506917 | None:intergenic | 60.0% | |
| AGGCCCGCTGAGTATGCCAT+TGG | - | chr1.4:29506897-29506916 | MS.gene053465:CDS | 60.0% | |
| CCGCTGAGTATGCCATTGGG+TGG | - | chr1.4:29506901-29506920 | MS.gene053465:CDS | 60.0% | |
| GGCCCGCTGAGTATGCCATT+GGG | - | chr1.4:29506898-29506917 | MS.gene053465:CDS | 60.0% | |
| GGTCTACCGGTAGGCGGACG+AGG | - | chr1.4:29506994-29507013 | MS.gene053465:CDS | 70.0% | |
| GCGGGTCGCCAGTGACCACG+TGG | + | chr1.4:29506070-29506089 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 29506025 | 29507020 | 29506025 | ID=MS.gene053465 |
| chr1.4 | mRNA | 29506025 | 29507020 | 29506025 | ID=MS.gene053465.t1;Parent=MS.gene053465 |
| chr1.4 | exon | 29506870 | 29507020 | 29506870 | ID=MS.gene053465.t1.exon1;Parent=MS.gene053465.t1 |
| chr1.4 | CDS | 29506870 | 29507020 | 29506870 | ID=cds.MS.gene053465.t1;Parent=MS.gene053465.t1 |
| chr1.4 | exon | 29506025 | 29506278 | 29506025 | ID=MS.gene053465.t1.exon2;Parent=MS.gene053465.t1 |
| chr1.4 | CDS | 29506025 | 29506278 | 29506025 | ID=cds.MS.gene053465.t1;Parent=MS.gene053465.t1 |
| Gene Sequence |
| Protein sequence |
